LOCUS       NZ_JAMXJO010000001     10744 bp    DNA     linear   CON 29-AUG-2024
DEFINITION  Nocardiopsis dassonvillei strain RACA_4 assembly, whole genome
            shotgun sequence.
ACCESSION   NZ_JAMXJO010000001
VERSION     NZ_JAMXJO010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nocardiopsis dassonvillei
  ORGANISM  Nocardiopsis dassonvillei
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsaceae; Nocardiopsis.
REFERENCE   1  (bases 1 to 6726007)
  AUTHORS   Elfeky,H.H., Hanora,A. and Solyman,S.M.
  TITLE     Bioactivity of bacteria associated with Red Sea nudibranchs and
            whole genome sequence of Nocardiopsis dassonvillei RACA-4
  JOURNAL   Mar Genomics 67, 101004 (2023)
   PUBMED   36521348
REFERENCE   2  (bases 1 to 6726007)
  AUTHORS   Elfeky,H., Hanora,A.S. and Solyman,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2022) Microbiology, Suez Canal University, 4 Km
            Circular Road, Ismailia 41511, Egypt
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Assembly Name                     :: SCU_Nocdas_4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: GCA_900638215.1
            Genome Coverage                   :: 7.6x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024181525.1-RS_2024_08_29
            Annotation Date                   :: 08/29/2024 06:43:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,838
            CDSs (total)                      :: 5,775
            Genes (coding)                    :: 5,721
            CDSs (with protein)               :: 5,721
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 7 of 54
            Pseudo Genes (incomplete)         :: 51 of 54
            Pseudo Genes (internal stop)      :: 0 of 54
            Pseudo Genes (multiple problems)  :: 4 of 54
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:23:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6571701
            Orig. end                         :: 6582445
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAMXJO010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10744
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="22"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10744
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10744
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5744](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5744)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            215..985
                     /locus_tag="NGM33_RS27760"
                     /old_locus_tag="NGM33_27760"
     CDS             215..985
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1171:
                     transcriptional regulator, MerR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019611516.1"
                     /locus_tag="NGM33_RS27760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_27760"
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_253797347.1"
                     /transl_table=11
                     /translation="MAWSTRQLAELAGTTLRTVRHYHEVGLLDEPERGANGYKRYKVEH
                     LVRVLRVKRLSDLGFSLVQIAEMGEADAYPEQELRALDDELAQAIERLRRMREELALAL
                     RGSGPTELPPDMALATAGIAMTEADRSLAAVIARLFTPQTQRALTELARAYGSGASDEE
                     FNNLPTDADERTRHELAERLWRASRDVLSDRPELRISQATTAIDRHKAAQAFTTALNDL
                     YNPAQLDVLNRLSRAATAELEAEQQADRQTGQRS"
     misc_feature    260..262
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(1094..2986)
                     /locus_tag="NGM33_RS27765"
                     /old_locus_tag="NGM33_27765"
     CDS             complement(1094..2986)
                     /EC_number="3.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013152312.1"
                     /locus_tag="NGM33_RS27765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_27765"
                     /product="S9 family peptidase"
                     /protein_id="WP_253797348.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 9.7e-07, bitscore:
                     28.0, seeds: 455, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S9 (E-value: 2.2e-47, bitscore:
                     159.6, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALPDLIPVEDFFSPPERADATISPDGARIAYLAPWKDRLNVWVQ
                     DLDPSGGLDGEPRCVTADETRSVQSYQWTDDPRWLLYLQDSGGDENWHLFRVDLDAPGS
                     AAVDLTPFPGARVVSFEPSRGRPGRMTVSLNARDAAEFDLHELDVSTGELTMLAQSPGA
                     ARIWTRGREGELLSTTLNENSDFEVSRHDSATGTTSGVLVYEGADYPLGVFPAQVTPDG
                     TGMWIGSSRGTDRTRLVRADLSTGEEIEVDSHPDFDIDTRAQVSTLPPPLILDHRGELL
                     GVRYLGERQVVHALDPHFAEVLANLEKLSEGDVAGLSSDTGGRRWVVSFTHDRDPGATW
                     FYDHSTGESRLLFRPHPHLDPDALAPMTAVTIPARDGLELPSYLTLPVGVEPENLPMVL
                     MVHGGPWARDAWGFDPTVQLLANRGYAVLQVNFRGSVGFGKAHMKAAIGEFAGKMHDDL
                     IDAVDWAVARGYADPDRVAIFGGSYGGYAALVGVTFTPDRFAAAIDYVGISDLANFMRN
                     QPAFVRPALANNWYRYVGDPAVPEQEADMLARSPITRVDRITTPLLVAQGANDARVVKA
                     ESDNIVAALRERGVDVEYLLKEDEGHGFVNPENQLDLHRAAERFLARHLGGRRN"
     misc_feature    2958..2971
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     gene            complement(3156..4991)
                     /locus_tag="NGM33_RS27770"
                     /old_locus_tag="NGM33_27770"
     CDS             complement(3156..4991)
                     /GO_component="GO:0005777 - peroxisome [Evidence IEA]"
                     /GO_function="GO:0003997 - acyl-CoA oxidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF046956.1"
                     /locus_tag="NGM33_RS27770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_27770"
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="WP_253797349.1"
                     /transl_table=11
                     /translation="MPPTTTGERDRLRALLHGAPRGGAEIAALFDHPVFAPADFSSAEE
                     FHRLTYERLRVLNGRVGGGRQALRSPERLLDVLGHAAVVDPSLFLRAMVHYEVCLNAAL
                     TLGDDNPGLHEAVDRLDDLSSVGSVLITEVGRGGSHVSVRTRAEYDPSLPGFRITTPEP
                     EDAKFMGTVGTPGIAQTGLVYAQLYTGGGQHGVFPFLVDISDEHGPRPGVRVTRLPHSG
                     VSPLDHCLVSFRDAPVPFGRWLRDSAGISPQGLFEEPPSHRGGRLTRSLAVSSAASAAG
                     ATALAAVTRATAGISYRYSRHRVTVSAHAGEVPVIGYGIQQESVYGALAAAYATTFLVR
                     GARARGGPPRVGPEGGGRDGGAAAWTPWASVSRELSLAKTAATEAAERACAESRRRCGA
                     QGALDANRISSYEALTHVYNSAGGENRLILLDVGRALVLEEDGERPRPEPPPGRSPGDP
                     RMWSYLFAEDERRLLAHARSVLGGSAGAVAPPREWDRHLWLLLALASAHSRRVAFESFE
                     EAAAPLAGSPAGELLSPLFGLWSLDAIGDRAAEHLAHGSLTREEYLVMLDLRRSLLGEV
                     DAEALVDVLDLPFEQIGAPIAFPDYSRRLGADGRP"
     gene            complement(5001..5744)
                     /locus_tag="NGM33_RS27775"
                     /old_locus_tag="NGM33_27775"
     CDS             complement(5001..5744)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF015700.5"
                     /locus_tag="NGM33_RS27775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_27775"
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_130397336.1"
                     /sec_met_domain="AfsA (E-value: 2.1e-06, bitscore: 26.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDERFWVVGDGFPTPRLHPEIRTYREVLHALRTGELREGYLLPAL
                     GVGPRDWEYLDQAVAEAGRSRTVFLDAPPPAPLPRAEVLKTIQENVVIARPRVSGHTVG
                     CDLVVADGNEIMHDHTAERQHLPGMLLIEAGIQATTWATRALYPSGEGRLPPYPVMHEC
                     AFEFERFLFWLPVEVRVTLVPQGPASGDRRPLRAEVSYLQQGRVAATGRFSFHAFDPPD
                     IHRIEQSQSRRVIESARDESRTASG"
     gene            complement(5737..6573)
                     /locus_tag="NGM33_RS27780"
                     /old_locus_tag="NGM33_27780"
     CDS             complement(5737..6573)
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (14-181). E-value: 9.1e-10.
                     Score: 30.7. Matches aSDomain:
                     nrpspksdomains_NGM33_RS27780_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019611520.1"
                     /locus_tag="NGM33_RS27780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_27780"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_253797350.1"
                     /sec_met_domain="adh_short (E-value: 5.9e-31, bitscore:
                     106.2, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 2.2e-36, bitscore:
                     124.4, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVMQEFRLDGARALVTGASQGIGRATALALAEAGAHVGLCARNG
                     PALEEVAREISGLGREAVVVPGDLREPSTAADAVEATAAALGGLDLLVHNAGGPHTDAD
                     GSMVLRPVTETSDEEWRGVVDLNLLAAVRLCRAAHPHLRRSERACVLLMSSAAALVAVP
                     GMESYGAVKSALLSYTRSLAVAWSGDGVRVNALCPGWVRTQLTRPVHGTEAMAGAALAN
                     VPMRRWGGVEDVTGPAVFLCSPAAAFVTGQYLAVDGGLSAFPAMPQPAPPQEAPHG"
     aSDomain        complement(6031..6531)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NGM33_RS27780_PKS_KR.1"
                     /evalue="9.10E-10"
                     /label="NGM33_RS27780_PKS_KR.1"
                     /locus_tag="NGM33_RS27780"
                     /protein_end="181"
                     /protein_start="14"
                     /score="30.7"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="LVTGASQGIGRATALALAEAGAHVGLCARNGPALEEVAREISGLG
                     REAVVVPGDLREPSTAADAVEATAAALGGLDLLVHNAGGPHTDADGSMVLRPVTETSDE
                     EWRGVVDLNLLAAVRLCRAAHPHLRRSERACVLLMSSAAALVAVPGMESYGAVKSALLS
                     YTRS"
     CDS_motif       complement(6466..6531)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NGM33_RS27780_0001"
                     /evalue="7.20E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="NGM33_RS27780"
                     /protein_end="36"
                     /protein_start="14"
                     /score="15.5"
                     /tool="antismash"
                     /translation="LVTGASQGIGRATALALAEAGA"
     gene            complement(6570..7601)
                     /locus_tag="NGM33_RS27785"
                     /old_locus_tag="NGM33_27785"
     CDS             complement(6570..7601)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) fabH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633272.1"
                     /locus_tag="NGM33_RS27785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_27785"
                     /product="3-oxoacyl-ACP synthase III family protein"
                     /protein_id="WP_253797351.1"
                     /sec_met_domain="fabH (E-value: 1.7e-86, bitscore: 288.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPVRISGVGEYLPAEEVSNERISEHAGVSAEWISQRTGVLARRRA
                     APDEATSDLAARACERALASAGLRPDQVGLIVSATSLPDELGPSVACRIQSRIGARDAA
                     AMDVGAACSGFLYALEVARCWLHARPGSPPALVVGAEVYSRFVDFADRATASLFADGAG
                     AVVLTSGDGGEGLSPLRLGADGWGADLVGVFSGGTRRPASPETVASGGHTIRMNGRAIS
                     DFTLRVLPRMVQECLEEHALALDDIDLVVSHQPNPLLLARACAQAGVPPERVLITGDLV
                     GNIGAGSVPFGLARAEATGRLREGDRVLLMAFGAGMTWGCGLLTWTGTERGRTRGEDGG
                     DLT"
     misc_feature    complement(7929..7940)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.51"
                     /tool="antismash"
     gene            complement(8276..8791)
                     /locus_tag="NGM33_RS27790"
                     /old_locus_tag="NGM33_27790"
     CDS             complement(8276..8791)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019611522.1"
                     /locus_tag="NGM33_RS27790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_27790"
                     /product="ATP-binding protein"
                     /protein_id="WP_130397339.1"
                     /transl_table=11
                     /translation="MTVLPRPRRPLDLDRRWKRRSYSANLAAIRRARADLRADLSRISG
                     ADGELVAAVVLCASEMLTSAMGRVLPGGSGAEVVRTLVLRHDPGGRRVLRLSVADRWIP
                     GALASVPGPEADQDWRELALGQGLLLIGHFSARWGTRRAGAPGEDGGRVIWAEFDLAPR
                     WSPRPVAA"
     gene            complement(8961..10106)
                     /locus_tag="NGM33_RS27795"
                     /old_locus_tag="NGM33_27795"
     CDS             complement(8961..10106)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PCMT"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019611523.1"
                     /locus_tag="NGM33_RS27795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_27795"
                     /product="methyltransferase domain-containing protein"
                     /protein_id="WP_130397340.1"
                     /sec_met_domain="PCMT (E-value: 2.2e-30, bitscore: 104.3,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIPPHERMVERLVEEGALAAEWRGAFAAVPRHLFLPDEIADPDTG
                     VPVDRYRGELRWLEAAYADVPVVTQVDDGARYGPGEPTSSCPAPSSVAETVQRLRLAPG
                     MRVLEVGTGSGYTAALLARFLGDDAVTSVEIDFELAEQARVRLMSAGLTPLVVSGDGMR
                     GWAGRAPYDRVVSAVTVQRVPYAWVAQCQPGGRVLTPWGTAFHHGVTADLVVGSHGTAH
                     GRFTGHTDRMWARADRTPRHLLETYVRPEDDEYTTTCSRLHPGEVVGDSDGAFAVGLQM
                     PDVHRIVRLGGGPEDPRFTVYLLDSSTWSWASWHIDPGHRDRGYEVRQHGPRRLFDELE
                     AAHLLWVEAGRPAHTRFGLTVSAEHQLVWLDDEATIFAAMR"
     misc_feature    complement(10066..10081)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.48"
                     /tool="antismash"
ORIGIN
        1 gccgccgcgg cccccgccgt ccggcctagg agtactccgt gcaccggtcg ccggtgcgcg
       61 ggagccgtgt gcccggccgt gcaactcccg ggcgggcacg cggtcctcgt cccacgacga
      121 ccgcacacgc ggtacgggtc gtggagcgcg gggtctgcac tagcgttccc ggtggagcga
      181 agaccgccgg accgtgagcg cggaggggac cgtcatggcg tggagcaccc gtcaactggc
      241 cgaactggcc ggaacgacct tacggacggt gcggcactac cacgaggtcg gtctgctgga
      301 cgagcccgag cgcggcgcca acggctacaa gcgctacaag gtcgagcacc tggtgcgggt
      361 cctgcgcgtc aaacgcctgt cggacctggg gttctccctg gtgcagatcg cggagatggg
      421 ggaggccgac gcctaccccg agcaggagct gcgggccctg gacgacgaac tcgcccaggc
      481 catcgaacgc ctgcggcgga tgcgcgagga gctggccctg gcgctgcgcg ggtccgggcc
      541 gaccgaacta ccacccgaca tggccctggc caccgccggg atcgccatga ccgaggccga
      601 ccgttcgctg gccgcggtca tagcgcgcct gttcacgccg cagacgcagc gggccctcac
      661 ggagttggct cgggcctacg gctccggtgc ctccgacgag gagttcaaca acctgcccac
      721 cgacgccgac gagcggaccc ggcacgagct cgccgaacgg ctgtggcgcg cctcccgcga
      781 cgtcctgtcc gaccgtccgg agctgcgcat ctcccaggcc acgacggcca tcgaccggca
      841 caaggccgcg caggcgttca ccacggcgct gaacgacctg tacaaccccg cccagctcga
      901 cgtcctcaac cgtctctccc gcgcggcgac ggcggagctt gaggccgaac agcaggccga
      961 ccggcagacc ggccagcggt cctgaccggt cgggcggccc gcggtgcccg cgggccgccg
     1021 gtcctgcccg gccgggggcg cggtggaggg ggtgggcccc ggccgccccc gtgcggccag
     1081 ggcccggccg ggctcagttc cggcgtccgc cgagatggcg ggcgaggaag cgctcggccg
     1141 cgcggtggag gtcgagctgg ttctccgggt tgacgaaccc gtgtccctcg tcctccttga
     1201 gcaggtactc cacgtccacg ccgcgctcgc gcagcgcggc gacgatgttg tcggactcgg
     1261 ccttgaccac gcgggcgtcg ttggccccct gcgccaccag cagcggtgtg gtgatccggt
     1321 ccaccctggt gatcggtgag cgggccagca tgtcggcctc ctgctcgggg acggccgggt
     1381 cgcccacgta gcggtaccag ttgttggcca gcgcgggccg cacgaacgcc ggctggttgc
     1441 gcatgaagtt ggccaggtcg gagatgccga cgtagtcgat ggcggcggcg aagcggtcgg
     1501 gggtgaaggt gacccccacg agcgcggcgt agcccccgta ggagccgccg aagatcgcga
     1561 cccggtccgg gtcggcgtaa ccccgcgcga cggcccagtc cacggcgtcg atgaggtcgt
     1621 cgtgcatctt cccggcgaac tcgccgatgg cggccttcat gtgggccttg ccgaacccga
     1681 cggagccgcg gaagttgacc tggagtacgg cgtagccccg gttggccaac agctgcacgg
     1741 tcgggtcgaa accccaggcg tcccgcgccc acgggcctcc gtgcaccatc agcaccatcg
     1801 gcaggttctc gggctccacg cccaccggca gggtcaggta cgagggcagt tccagcccgt
     1861 cccgggcggg gatcgtgacg gccgtcatcg gcgccaacgc gtcggggtcc aggtgcgggt
     1921 gggggcggaa cagcagacgg gactcgcccg tggagtggtc gtagaaccag gtcgcgcccg
     1981 ggtcgcggtc gtgggtgaag ctcacgaccc acctccgccc gccggtgtcg gacgacaggc
     2041 ccgcgacatc cccctcggac agcttctcca ggttggccag cacctcggcg aagtgcgggt
     2101 ccagggcgtg gacgacctgg cgttcgccca ggtagcgcac gcccagcagc tccccccggt
     2161 ggtcgaggat cagcggcggg ggaagggtgg agacctgtgc gcgcgtgtcg atgtcgaagt
     2221 ccgggtggct gtccacctcg atctcctcac ccgtggacag gtcggcccgt accaggcggg
     2281 tgcggtcggt gccccggctg gagccgatcc acatgccggt cccgtccggt gtgacctgag
     2341 cggggaagac ccccagcggg tagtcggcgc cctcgtacac caggacgccg ctcgtggtgc
     2401 ccgtcgcgga gtcgtgacgg gagacctcga agtcgctgtt ctcgttgaga gtggtggaga
     2461 gcagttcgcc ctcccgcccc cgcgtccaga tcctcgcggc gccggggctc tgggcgagca
     2521 tggtgagctc gccggtggac acgtccaact cgtgcaggtc gaactcggcg gcgtcgcggg
     2581 cgttgagcga cacggtcatc cttccgggcc gtccccggga gggctcgaag ctcaccacgc
     2641 gggcgccggg gaagggggtc aggtccaccg ccgccgaacc cggcgcgtcc agatcgaccc
     2701 ggaacaggtg ccagttctcg tcgcctccgc tgtcctggag gtagagcagc cagcgggggt
     2761 cgtcggtcca ctggtagctc tgcacgctgc gggtctcgtc ggcggtcacg cagcgcggtt
     2821 cgccgtccaa gcccccggac gggtccaggt cctggaccca gacgttgagc cggtccttcc
     2881 acggcgccag gtaggcgatc cgcgcgccgt ccggcgagat ggtcgcgtca gcgcgctccg
     2941 gcgggctgaa gaagtcctcg accgggatca ggtcgggcag tgccatgtcg ttcctctcgt
     3001 tcgtgctcgg tgaggcaggg gagccgaaga tcaggagaaa ccatgccgac gcggcacact
     3061 caacaacccg cgcgtgtgac gggagacacc tttccggatc gggggaggac gtccggcgtt
     3121 caccggaggg cgcgcggctc cgggagaggc ggccgtcacg ggcggccgtc cgcgcccagc
     3181 cgacgggagt agtcgggaaa cgcgatcggc gccccgatct gttcgaacgg caggtcgagt
     3241 acgtccacga gcgcctccgc gtcgacctcg ccgagcaggg accggcgcag gtcgagcatg
     3301 accaggtact cctcgcgggt gaggctgccg tgggccaggt gctcagccgc ccggtcgccg
     3361 atggcgtcga gcgaccacag cccgaacagg ggcgagagga gttccccggc gggtgacccg
     3421 gcgagcgggg ccgcggcctc ctcgaaggac tcgaaggcca cccgccgcga gtgcgcggag
     3481 gcgagcgcga gcagcagcca caggtgcctg tcccactctc gcgggggcgc gaccgccccg
     3541 gcggagccgc cgaggacgga gcgggcgtgc gcgagcaggc gccgctcgtc ctcggcgaac
     3601 aggtacgacc acatccgcgg gtcccccgga gagcggcccg ggggaggctc cgggcggggc
     3661 cgctccccgt cctcctccag caccagtgcg cgccccacgt ccaggaggat gagccggttc
     3721 tccccgcccg cgctgttgta gacgtgggtg agggcctcgt aggaggagat cctgttggcg
     3781 tccagggcgc cctgggcgcc gcagcgccgc cgggactcgg cgcaggccct ctccgccgcc
     3841 tcggtggccg cggtcttggc cagggacagc tcccggctca ccgaggccca gggggtccag
     3901 gccgccgcgc cgccgtctcg tccgccgccc tccggtccga cccggggcgg accgccgcgg
     3961 gcgcgtgcgc cgcggaccag gaaggtggtg gcgtaggcgg cggcgagcgc accgtacacg
     4021 gactcctgct ggatcccgta gccgatgacg ggcacctccc cggcgtgcgc gctcaccgtg
     4081 acccggtgcc gcgagtaccg gtaggagatg ccggcggtcg cccgggtcac ggccgccagg
     4141 gccgtggcgc cggccgccga ggccgcggag gagaccgcca gcgaacgcgt gagccgtccg
     4201 ccccggtggg agggcggttc ctcgaagagc ccctgcgggg agatcccggc gctgtcccgc
     4261 agccagcggc cgaacggtac gggcgcgtcc cggaaggaga ccaggcagtg gtccagcggg
     4321 gagacaccgc tgtggggcag gcgcgtcacc cgtacgccgg ggcggggtcc gtgctcgtcg
     4381 ctgatgtcga ccaggaaggg gaagaccccg tgctggccgc cgccggtgta cagctgtgcg
     4441 tacaccaggc ccgtctgcgc gatcccgggg gtcccgaccg tgcccatgaa cttggcgtcc
     4501 tccggttcgg gggtcgtgat cctgaagccg ggcaggctcg ggtcgtactc ggcccgggtg
     4561 cgcacggaga cgtggctgcc gccgcgccct acctcggtga tcaggacgct gccgaccgag
     4621 gagaggtcgt cgagccggtc cacggcctcg tgcagaccgg ggttgtcgtc gccgagggtg
     4681 agggcggcgt tgaggcacac ctcgtagtgc accatcgcgc ggaggaagag ggaggggtcg
     4741 accaccgcgg cgtgcccgag cacgtcgagg agtcgttcgg gcgagcgcag ggcctgcctg
     4801 ccccctccga cgcgcccgtt gaggacgcgc aggcgttcgt aggtgaggcg gtggaactcc
     4861 tcggcggagg agaagtcggc cggcgcgaag accgggtggt cgaacagggc ggcgatctcc
     4921 gccccaccgc ggggcgcgcc gtgcaggagc gcgcggagcc ggtcgcgctc cccggtggtc
     4981 gtcggtggca tctgtcctcc tcagccggac gcggtccggc tctcgtcgcg ggcgctttcg
     5041 atcaccctcc gtgactgact ctgttctatg cggtggatgt cgggagggtc gaaggcgtgg
     5101 aaggagaaac gtcccgtggc ggcgacacgc ccctgttgca ggtaggagac ctccgcgcgc
     5161 agtggccgtc tgtcgccgga ggccggcccc tggggaacga gggtgacgcg gacctcgacc
     5221 ggcagccaga acaggaaccg ctcgaactcg aacgcgcact cgtgcatgac cgggtacggg
     5281 gggagccgcc cctccccgga cgggtacagt gcccgggtgg cccaggtcgt ggcctggata
     5341 cccgcctcga tgagcagcat gcccggcagg tgctgccgct cggcggtgtg gtcgtgcatg
     5401 atctcgttgc cgtcggcgac caccaggtcg cacccgaccg tgtggccgct cacccgcggc
     5461 cgtgcgatga ccacgttctc ctggatggtc ttgagcacct cggcgcgcgg gagcggcgcg
     5521 ggaggagggg cgtcgaggaa cacggtccgc gaccgccccg cctccgcgac ggcctggtcc
     5581 aggtactccc agtcccgggg gccgacgccg agggcgggca gcaggtaccc ctcgcgcagc
     5641 tcgccggtgc ggagcgcgtg gaggacctcc cggtaggttc tgatctcggg gtggagccgg
     5701 ggcgtgggga acccgtcgcc gacgacccag aaccgttcat ccatgcgggg cctcctgagg
     5761 gggcgcgggc tggggcatgg cggggaaggc cgacaggccc ccgtccaccg ccaggtactg
     5821 gccggtgacg aaggccgcgg ccggggagca gaggaacacg gcggggccgg tgacgtcctc
     5881 gacccctccc caccggcgca tcggcacgtt ggcgagggcg gccccggcca tggcctcggt
     5941 cccgtggacg ggcctggtga gctgggtgcg cacccagccg gggcacagcg cgttgacgcg
     6001 taccccgtcc ccggaccacg ccacggccag ggaacgcgtg taggagagca gggcgctctt
     6061 gaccgccccg taggactcca tgcccggaac ggcgacgagc gccgccgcgg aggacatgag
     6121 caggacgcag gcgcgttcgg agcgccgcag gtgggggtgg gccgcgcggc acagccgcac
     6181 cgcggccagc aggttgaggt ccacgacccc ccgccactcc tcgtccgagg tctcggtgac
     6241 ggggcgcagg accatgctcc cgtcggcgtc ggtgtggggg ccgcccgcgt tgtgcacgag
     6301 caggtccagc ccgcccaggg ccgcggcggt cgcctccacg gcgtcggcgg ccgtcgacgg
     6361 ctccctcagg tccccgggca cgaccacggc ctcgcgcccg aggccggaga tctcccgcgc
     6421 cacctcctcc agggccggtc cgttgcgcgc gcacagcccc acgtgcgccc ccgcctcggc
     6481 cagcgcgagg gcggtggccc ggccgatgcc ctgggaggca ccggtgacca gtgccctggc
     6541 accgtcgagg cggaactcct gcatgacgct catgtgagat ctcctccgtc ttcgccgcgg
     6601 gtgcggccgc gttcggtccc ggtccaggtc agcaggccgc agccccaggt catgccggcg
     6661 ccgaaggcca tgagcaggac gcggtccccc tcgcgcaggc gtccggtggc ctcggcccgg
     6721 gcaaggccga acggcaccga tccggcgccg atgttgccca cgaggtcacc ggtgatgagg
     6781 acccgttcgg ggggcactcc cgcctgcgcg caggccctgg ccagcagcag cgggttcggc
     6841 tggtgcgata ccaccaggtc gatgtcatcc agggcgaggg cgtgctcctc caggcactcc
     6901 tggaccatgc gcggcagcac acggagggtg aagtcgctga tggccctgcc gttcatgcgg
     6961 atggtgtggc cgccggaggc gacggtttcc ggggaggccg ggcgccgggt gcctccggag
     7021 aagaccccga ccaggtcggc cccccacccg tccgctccca gccggagggg ggacagcccc
     7081 tccccgccgt cccccgaggt cagcaccacg gctccggccc cgtccgcgaa gagggaggcc
     7141 gtggcccggt cggcgaagtc gacgaaacgc gagtacacct cggctccgac caccagggcg
     7201 ggcggggagc ccggcctggc gtgcagccag caccgcgcga cctcgagcgc gtacaggaat
     7261 ccgctgcacg cggctcccac gtccatggcg gccgcgtcgc gggcgccgat gcggctctgg
     7321 atccgacagg ccaccgaggg gccgagctcg tcgggcaggg acgtggcgct gacgatcagc
     7381 ccgacctggt ccggccgcag tccggcggag gccagcgcac gctcgcaggc ccgcgcggcc
     7441 aggtccgagg tcgcctcgtc cggggccgcg cgcctgcgcg ccagcacccc cgtgcgctgg
     7501 cttatccatt ccgcggagac tcccgcgtgc tctgatatcc gctcgttgga aacctcttct
     7561 gcgggcaggt attcgcctac tcctgatatt cgaacgggca agggggctcc agggcgctgg
     7621 gcggggaaaa ggggagagca aaaaggatca ttcccgtcgg atgccgcgga aaaactcctt
     7681 tactcctggt aggcgaagag ggagggcgtt tcctccgggg gtgctgtgga tccaggagtc
     7741 gggaggtcag gtgggctgtg gtgaccatgc ggattccggg cgtgcggaag ggttttcgag
     7801 ggtggttctc cgttgtttcg gtgggcgcgg gcgggttcgg gggcccggtt cgttctggga
     7861 attcgggggg ttcgggcccc ggcctgttcc tgtacgggaa agggctgtat ccggttccgg
     7921 gtcgcgggga gaacattttc tccggggagt tccgtcctcg gaagaaccgc ccggacacgg
     7981 caggtgcgta gctggtggat atgtgggcga gatgacatgg gaacggtctg ccgccggcgc
     8041 ttcgcgggtg ccgggcgcac ctggacagga ctccgccgcc cggctcccgc tcacgtgccg
     8101 ggccccatgg cggaggagca cgtcgcgact ggacggggaa cctgcgccgc aggggaggag
     8161 gaccggcgcg gcggccgcct gcggcggcgc ggggaacgga ccgggaggag gagtcaccgc
     8221 tgaacggctc tgggcccgcg gccccgggcc cagctccaac ccggggcccg cacccctacg
     8281 ccgccaccgg gcgcgggctc caccgcggcg cgaggtcgaa ctcggcccag atcacgcggc
     8341 ccccgtcctc tcccggcgca ccggcccgac gggttcccca gcgggcggag aagtggccga
     8401 tgagcagcag cccctgcccc agcgcgagct cccgccagtc ctggtccgcc tccggacccg
     8461 gcacgctcgc cagggcgccc ggaatccacc ggtccgccac cgacagccgc aggacgcggc
     8521 gaccaccggg gtcatggcgc agcacgagcg tgcgcaccac ctccgccccg cttccgccgg
     8581 gcaggacccg ccccatggcg ctggtcagca tctcgctggc gcacagcacc acggccgcga
     8641 ccagttcccc gtcggcgccc gagatccgcg acaggtcggc gcgcaggtcg gcccgggctc
     8701 tgcggatggc cgccaggttg gcggagtagc tgcggcgctt ccagcgtctg tccaggtcga
     8761 gcggtcgtcg tgggcgtggc aggacggtca tctgtcttct cctcggttcg tgcgacggcc
     8821 gcacgggcgg ttcccacggg aacggcctcc acggtccgtc gggtcctggt gttctctcgt
     8881 gtccgtgtgc cttcgtgccg tggcgtcccc ggaaggcccc gggagcacgc ggtcccggtt
     8941 ccggcgctcc acccgccccg tcacctcatg gcggcgaaga tcgtggcctc gtcgtccagc
     9001 cacaccagct ggtgttcggc cgacaccgtc agcccgaacc gggtgtgggc gggacgcccc
     9061 gcctccaccc acagcaggtg ggccgcctcc aactcgtcga acagcctgcg ggggccgtgc
     9121 tggcgcacct cgtagccccg gtcccggtgc ccggggtcga tgtgccagga ggcccacgac
     9181 caggtcgagg agtccagcag gtacaccgtg aaccgcgggt cctcggggcc gcccccgagc
     9241 cggacgatcc ggtgcacgtc cggcatctgc aaccccaccg cgaaggcccc gtcggagtcg
     9301 ccgaccacct ctcccggatg cagccgggaa cacgtcgtgg tgtactcgtc gtcctccggc
     9361 cggacgtagg tctccagcag gtggcgcggg gtgcggtccg cgcgcgccca catgcggtcg
     9421 gtgtgcccgg tgaacctgcc gtgcgccgtc ccgtgcgagc cgaccacgag atcggccgtc
     9481 accccgtggt ggaaggcggt gccccacggc gtcaggacgc gtccgccggg ctggcactgc
     9541 gccacccagg cgtaggggac gcgctgcacg gtcaccgcgc tgaccacccg gtcgtagggc
     9601 gcgcgcccgg cccagccgcg catgccgtcc ccgctgacca ccaaaggtgt gaggcccgcg
     9661 ctcatcagcc gtacccgcgc ctgttcggcc agttcgaagt cgatctccac cgaggtgacg
     9721 gcgtcgtcgc ccaggaaccg cgcgagcagg gcggcggtgt acccgctgcc ggtgcccacc
     9781 tccagcaccc gcatccccgg cgccagacgc agccgctgca ccgtctcggc caccgaggac
     9841 ggcgccgggc acgaggacgt gggctctccg gggccgtagc gggcgccgtc gtcgacctgg
     9901 gtgacgacgg gaacgtcggc gtaggccgcc tccagccacc gcagttcgcc gcggtagcgg
     9961 tcgacgggca cgcccgtgtc ggggtcggcg atctcgtcgg gcaggaacag gtgccgcggc
    10021 accgccgcga aggccccgcg ccactccgcg gccagagcgc cctcctcgac gaggcgttcg
    10081 accatcctct cgtgcggggg aatcatctgc cgcacctccc gcaccgtctg acgcactgtg
    10141 cgctgacgcc ccgggcacgc cgggcgtgcc cgacgcgtcc gccgtacctg gtgcgctcgc
    10201 cgtatccgac aggtccggag tgtccggcgg acggagcgcg ctcggcgtac ccggggtgct
    10261 cgacgcttcc atcgcgtccg gggagtccgg cggacggagc gtgtccggcg tatccggggt
    10321 gtccgcagcg cccgctccgg tcgtgccttc cgcgccgccg gaagcgcgcg tcactcgtgt
    10381 gaacagcacc gacaccggct ccggacccgt ggcccgcgcc gccgccgtgc cctgcctgtg
    10441 gggtggaggt cacgctcttc tcctcggggg gtgcccccgg catggacgaa ccagggtctt
    10501 gcgtccatta cggggacggg agtttccggg taaggattac tacggaagat gtaacgccgt
    10561 tcgtccacga ggacggaatt ccgtgccgat atacaccggc ggtatgcatg acgaaatccc
    10621 aggtcggtcc acgtctttcc ccgtcacggg ctttctgggc ccgtgccgac gtgctgcgtt
    10681 cgctggccga gcacgacgcc ggggccctgt tcgggctcgt ccgacagcac accggtgcca
    10741 gcca
//
