LOCUS       NZ_JAMXJO010000001     10396 bp    DNA     linear   CON 29-AUG-2024
DEFINITION  Nocardiopsis dassonvillei strain RACA_4 assembly, whole genome
            shotgun sequence.
ACCESSION   NZ_JAMXJO010000001
VERSION     NZ_JAMXJO010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nocardiopsis dassonvillei
  ORGANISM  Nocardiopsis dassonvillei
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsaceae; Nocardiopsis.
REFERENCE   1  (bases 1 to 6726007)
  AUTHORS   Elfeky,H.H., Hanora,A. and Solyman,S.M.
  TITLE     Bioactivity of bacteria associated with Red Sea nudibranchs and
            whole genome sequence of Nocardiopsis dassonvillei RACA-4
  JOURNAL   Mar Genomics 67, 101004 (2023)
   PUBMED   36521348
REFERENCE   2  (bases 1 to 6726007)
  AUTHORS   Elfeky,H., Hanora,A.S. and Solyman,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2022) Microbiology, Suez Canal University, 4 Km
            Circular Road, Ismailia 41511, Egypt
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Assembly Name                     :: SCU_Nocdas_4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: GCA_900638215.1
            Genome Coverage                   :: 7.6x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024181525.1-RS_2024_08_29
            Annotation Date                   :: 08/29/2024 06:43:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,838
            CDSs (total)                      :: 5,775
            Genes (coding)                    :: 5,721
            CDSs (with protein)               :: 5,721
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 7 of 54
            Pseudo Genes (incomplete)         :: 51 of 54
            Pseudo Genes (internal stop)      :: 0 of 54
            Pseudo Genes (multiple problems)  :: 4 of 54
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:23:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5025450
            Orig. end                         :: 5035846
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAMXJO010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10396
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="17"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10396
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10396
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5396](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5396
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(57..71)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.0"
                     /tool="antismash"
     gene            complement(444..1124)
                     /locus_tag="NGM33_RS21580"
                     /old_locus_tag="NGM33_21580"
     CDS             complement(444..1124)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017598649.1"
                     /locus_tag="NGM33_RS21580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_21580"
                     /product="copper resistance CopC family protein"
                     /protein_id="WP_253796186.1"
                     /transl_table=11
                     /translation="MTTTRTPDTAAPARALPRAAAAVLAPLAAAALALAPSPALAHDVL
                     TGSNPEDGATLDTVPEEVVLSFNNSPMEGGSGSAVVVTGPDEETTYEEGDLTFDGTDVS
                     VGLAPLDQAGEYTIGFRVVSSDGHPIQDTLTFSVTEEAVAAAAPEPEESEPAQEPAGET
                     AQEQAQEPSDEANADEAAAEEDSGGVSPVALAVVAVVAVAGIAAVVLVAVRMRRRPGGD
                     AGQK"
     gene            complement(1121..1747)
                     /locus_tag="NGM33_RS21585"
                     /old_locus_tag="NGM33_21585"
     CDS             complement(1121..1747)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017616170.1"
                     /locus_tag="NGM33_RS21585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_21585"
                     /product="copper resistance protein"
                     /protein_id="WP_165435989.1"
                     /transl_table=11
                     /translation="MRPAHGTLRLLRTALLAAACTGLAWGGHNLWAAAPASLAGLAAAT
                     ALLFPLLWYFTRTMRGFGDIFAVMACVQILLHLVFQGSGEPVPGVLDTGADHTGHGLLT
                     HTLGLAPGMLFAHLWAALLASALLAHGEAALWFLTALLTRALPPLRVPGIAFGARVPVA
                     CAASRTAPPPVALSAHGPRGPPLPRASFHDAPARTPERTENRGKT"
     gene            complement(1937..2206)
                     /locus_tag="NGM33_RS21590"
                     /old_locus_tag="NGM33_21590"
     CDS             complement(1937..2206)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156198.1"
                     /locus_tag="NGM33_RS21590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_21590"
                     /product="WhiB family transcriptional regulator"
                     /protein_id="WP_013156198.1"
                     /transl_table=11
                     /translation="MNRLYHESEDWVLQGNCRSQDPEIFFPVSSTGIGRADTEQAIAVC
                     RSCTVRQECLRWALRAGEAHGVWGGTTPEERRYMRRELVPAGRH"
     misc_feature    complement(2617..2634)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.81"
                     /tool="antismash"
     misc_feature    complement(2830..2839)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.85"
                     /tool="antismash"
     misc_feature    2844..2859
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 21.77"
                     /tool="antismash"
     misc_feature    2844..2860
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.26"
                     /tool="antismash"
     misc_feature    2896..2911
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.92"
                     /tool="antismash"
     misc_feature    complement(2896..2914)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.59"
                     /tool="antismash"
     gene            3019..3516
                     /gene="ectA"
                     /locus_tag="NGM33_RS21595"
                     /old_locus_tag="NGM33_21595"
     CDS             3019..3516
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610152.1"
                     /locus_tag="NGM33_RS21595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_21595"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_130396392.1"
                     /transl_table=11
                     /translation="MRLSHPSPEDGPHMWRLARENGMDANSPYAYALWCRDFATTSVVA
                     RDDEGRVAAYVTGYVRPDDPDTYFLWQVAVDSAYRGQGLARRMLDFVGDRITDRGLRYL
                     EATVTADNTASRALFASFARDRDAEAVWSPLFTGEHFPQEDTPHDPEDLVRIGPLGAGP
                     GA"
     gene            3606..4871
                     /gene="ectB"
                     /locus_tag="NGM33_RS21600"
                     /old_locus_tag="NGM33_21600"
     CDS             3606..4871
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (22-357). E-value: 7.8e-73.
                     Score: 237.3. Matches aSDomain:
                     nrpspksdomains_NGM33_RS21600_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610153.1"
                     /locus_tag="NGM33_RS21600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_21600"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_253796188.1"
                     /sec_met_domain="Aminotran_3 (E-value: 7e-74, bitscore:
                     247.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFQRLESEVRGYCRNWPVVFDRAVGSHVYSEDGKPYLDFFAGA
                     GSLNYGHNNPELKTSLIEYLTDDKIVHSLDAYSVAKREFLKTFEEIILKPRGLDYKVQF
                     PGPAGNNAVEAALKLARKYTGRETIVNFTNGFHGMTLGALAVTGNSMKRGGAGVPLGHV
                     ATMPFDNYLDGKTPDFLWLRSLLDDSGSGLDKPAAVIVETVQGEGGINAASAQWLRELS
                     DLCREYDILMIVDDIQMGCGRTGDFFSFEEAGIIPDIVTLSKSISGYGLPMALTLFKRE
                     LDVWEPGEHNGTFRGFNPAMVTAVEALRRYWSDSAFADSVKAKGDMVAARLAEMAAEHA
                     EFGAHVRGRGLARGLAFERTDIAKKVAAESFDRGLLLETSGPEDEVAKLLPPLTASEEE
                     LTVGLDIMADAARAAVKAAQPA"
     aSDomain        3672..4676
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NGM33_RS21600_Aminotran_3.1"
                     /evalue="7.80E-73"
                     /label="NGM33_RS21600_Aminotran_3.1"
                     /locus_tag="NGM33_RS21600"
                     /protein_end="357"
                     /protein_start="22"
                     /score="237.3"
                     /tool="antismash"
                     /translation="DRAVGSHVYSEDGKPYLDFFAGAGSLNYGHNNPELKTSLIEYLTD
                     DKIVHSLDAYSVAKREFLKTFEEIILKPRGLDYKVQFPGPAGNNAVEAALKLARKYTGR
                     ETIVNFTNGFHGMTLGALAVTGNSMKRGGAGVPLGHVATMPFDNYLDGKTPDFLWLRSL
                     LDDSGSGLDKPAAVIVETVQGEGGINAASAQWLRELSDLCREYDILMIVDDIQMGCGRT
                     GDFFSFEEAGIIPDIVTLSKSISGYGLPMALTLFKRELDVWEPGEHNGTFRGFNPAMVT
                     AVEALRRYWSDSAFADSVKAKGDMVAARLAEMAAEHAEFGAHVRGRGLARGLAF"
     gene            5001..5396
                     /locus_tag="NGM33_RS21605"
                     /old_locus_tag="NGM33_21605"
     CDS             5001..5396
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017567884.1"
                     /locus_tag="NGM33_RS21605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_21605"
                     /product="ectoine synthase"
                     /protein_id="WP_130396394.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.3e-54, bitscore:
                     181.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSMDEVNDTDADIKTENWRSRRIVLAKDKVGFSFHETVLYAG
                     TESTFWYANHVELVHCIEGEAELTNEETGEKHLITPGTLYLLDGHERHTVRPKTDFRVL
                     CVFNPPVTGREVHDENGVYPLVVEEDA"
     misc_feature    complement(5430..5444)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: strong, score: 28.31"
                     /tool="antismash"
     misc_feature    complement(5597..5612)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.68"
                     /tool="antismash"
     gene            5887..6735
                     /gene="ehuB"
                     /locus_tag="NGM33_RS21610"
                     /old_locus_tag="NGM33_21610"
     CDS             5887..6735
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033294 - ectoine binding [Evidence IEA]"
                     /GO_function="GO:0033295 - hydroxyectoine binding [Evidence
                     IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610155.1"
                     /locus_tag="NGM33_RS21610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_21610"
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="WP_130398922.1"
                     /transl_table=11
                     /translation="MGLATLGLAACTRVDGGGEGEEGASTLDRLREQGFVAVGLANEIP
                     FGFVDDSGNPAGQSPAVAKAVFEELGVPEIQASSVNWDGLIPGLNANRFDVIAAGMFIT
                     PERCGQVAFSEPTATSPEAFMVAEGNPENIQHFTDFADNPDLVLAVLNGSVEQTYAEYF
                     EVPEDQMELIPNQTAGYELLQAGRVDAMSMLSVSHTYLLQDRGGPFEVTEPFFPEIDGR
                     EEKGWGGLCLRQQDTQLLEEVNRVIAEFKESGRLLELGEEWGFTEADLPDDTTTAELCE
                     G"
     gene            6743..7471
                     /gene="ehuC"
                     /locus_tag="NGM33_RS21615"
                     /old_locus_tag="NGM33_21615"
     CDS             6743..7471
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuC"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156203.1"
                     /locus_tag="NGM33_RS21615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_21615"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="WP_253796196.1"
                     /transl_table=11
                     /translation="MDFLIQRLPLYAEGAWVTIQLTLGGIVLAFVLAMVFGIMGTIHHW
                     LPRAVATVYVEVFRGIAALVLMFWMAFALPQLTGYQLADNFAAILAVGLNLGAYGAEVV
                     RSSINAVPKPQVEATVALNLSWFQRTRLVVLPQAWAQMLPTFGNLNIELMKATAVSYLI
                     GVADLTAVAQNMRQATGETVIAFIGAFVLYYLIAQVLILGMRLLERRANRKLGRAAPGG
                     GGLASLLRPPAVDAKAGVAK"
     gene            7471..8109
                     /gene="ehuD"
                     /locus_tag="NGM33_RS21620"
                     /old_locus_tag="NGM33_21620"
     CDS             7471..8109
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuD"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156204.1"
                     /locus_tag="NGM33_RS21620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_21620"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="WP_130396396.1"
                     /transl_table=11
                     /translation="MFWDFEWTFEVMPDLLGGLWVTVQVTVLGYLIALVLGLAIAIARR
                     TPFFGPVLHAVMEFIRTTPLIIQLVFVFLLTPAGVSAMTVGVVVIGVHYAAYTAEVYRS
                     GIEGVAKGQWEAARALSLPASRTWGAIVLPQAIRRTIPALGNYLIAMFKDTPLLLAISV
                     PELLTEAQQVGSADFRIVEAYTLVGILFLLVSIPSAILIRRLERRYAAV"
     gene            8096..8932
                     /gene="ehuA"
                     /locus_tag="NGM33_RS21625"
                     /old_locus_tag="NGM33_21625"
     CDS             8096..8932
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610157.1"
                     /locus_tag="NGM33_RS21625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_21625"
                     /product="ectoine/hydroxyectoine ABC transporter
                     ATP-binding protein EhuA"
                     /protein_id="WP_253796205.1"
                     /transl_table=11
                     /translation="MPPSDTPVNEEPQGVGSGPRNQSLIVFDDVVKRFGDLTVLDHLNF
                     SVAPGERVTLIGPSGSGKTTILRLLMTLEKVTEGVIWVGGEPFSHMRKGQGLTRANESY
                     LRARRKRVGMVFQQFNLFPNMTVRQNVTEAPVHVLGTPKGEANAKAEELLELVGLGDKI
                     DAHPTQLSGGQQQRVAIARALAMQPEILLLDEVTSALDPELVAGVLDVLREVAETTDIT
                     MLCVTHEMGFARDVSHRVLMFDQGQIAEEGTPEQIFGDPKEERTKSFLRSVLDNGF"
     gene            9097..10029
                     /locus_tag="NGM33_RS21630"
                     /old_locus_tag="NGM33_21630"
     CDS             9097..10029
                     /EC_number="3.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156206.1"
                     /locus_tag="NGM33_RS21630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_21630"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_197044257.1"
                     /sec_met_domain="Abi (E-value: 1.7e-10, bitscore: 39.5,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVRRWGLVWGVLAALVFVTVPMLVHPVLRSDVLDDSLVLFLISWS
                     PMLIGGILAWAMVRGFGARDQLDRRVVQAMDGHPIGRELGWLGLFLAGFVVSMALLSMV
                     FATYEAVFGADLAMSEALVSRLLFLFTLPLLVMDRSGITLDGKGTAMPAVALKVSEPWR
                     WLGMVPIAIALVLIGYLLIPRLGLPGPSLQLLGYVLAFTVMAVCEEIFFRGMVQTRLEI
                     LMGRWGGIVTTSVVFALTYALIQPYDAVSQLPGADLVYNTGLALLTYATASLLYGYLWT
                     CFRNTWIQVVMRVGMFLMLMPPDLQTGVM"
     misc_feature    complement(10109..10124)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.37"
                     /tool="antismash"
     misc_feature    10145..10160
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.87"
                     /tool="antismash"
ORIGIN
        1 cgtgggccgg gacgacgcgg ccgaccccag cgcctggctc atgtgcagcg acggcctgtc
       61 cgacctggtc ctgcccgagg agatggagcg gatgatcgcc gaggcgggca gcgacgaggc
      121 cgccgtgtac gccctgtggc aggccgccat ggaggcgggc ggcaaggaca acatcagcat
      181 cctgctggca cgccgcgtct gacccgtccc gaccgccggc cccgacggtc ggccggcgtt
      241 cccgaccgag ccggtcccgc cccccgaccc ccctccaccc accagcctcc cccgacgcac
      301 ggcccccgcg cgagcggacg ccgccgacgc ccccgtgtcg gccctccccg tcgcagggcg
      361 ggcggaccgg gcaccgaaca cgacgagggg cccgcacgcc ctccccgcgg ggaggcgcgt
      421 acgggcccct cggccttccg gtactacttc tgcccggcgt ccccgcccgg gcgcctgcgc
      481 atgcgcacgg ccacgagcac gaccgcggcg atgccggcga ccgcgacgac ggccacgacc
      541 gccagcgcca ccggtgacac gccgcccgaa tcctcctcgg ccgcggcctc gtccgcgttc
      601 gcctcgtcgg agggctcctg cgcctgctcc tgcgccgtct ccccggcggg ctcctgcgcg
      661 ggctcgctct cctcgggctc cggcgccgcc gcggcgacgg cctcctcggt caccgagaac
      721 gtcagggtgt cctggatcgg gtggccgtcg gaggagacca cgcggaaccc gatggtgtac
      781 tcaccggcct ggtccagcgg ggccagaccc accgacacgt ccgtgccgtc gaaggtcagg
      841 tccccctcct cgtaggtggt ctcctcgtcc ggccccgtga cgacgacggc gctgccgctg
      901 ccgccctcca tcggcgagtt gttgaagctc agcacgacct cctcgggcac ggtgtccagg
      961 gtggccccgt cctccgggtt ggacccggtc aggacgtcgt gcgccagcgc gggcgagggg
     1021 gccagggcca gggcggccgc ggcgaggggg gcgagtacgg cggcggccgc gcgcgggagc
     1081 gcgcgtgcgg gggccgcggt gtcgggggtg cgggtggtgg tcatgtcttc cctcggttct
     1141 cggtacgttc cggcgtgcgg gcgggggcgt cgtggaagga cgcccgcggg aggggaggtc
     1201 cgcgcgggcc gtgcgccgag agcgcgacgg gcgggggagc ggtgcgggag gcggcgcacg
     1261 cgaccggtac gcgggcgccg aacgcgatgc cgggcacgcg cagcggcggc agggcccggg
     1321 tgagcagggc ggtcaggaac cacagggccg cctcgccgtg ggcgagcagc gccgacgcca
     1381 gcagggccgc ccacaggtgc gcgaacagca tgccgggcgc gaggccgaga gtgtgggtga
     1441 gcaggccgtg cccggtgtgg tcggcgccgg tgtcgaggac gccggggacg ggttcgcccg
     1501 aaccctggaa caccaggtgc aggaggatct gcacgcaggc catgacggcg aagatgtcgc
     1561 cgaacccgcg catcgtgcgg gtgaagtacc acagcagggg gaacaggagc gcggtcgccg
     1621 cggccagccc tgcgagggag gcgggcgccg cggcccacag gttgtgcccg ccccaggcaa
     1681 gccccgtgca cgcagcggcc aggagggccg tgcgcaggag gcggagtgtg ccgtgagcgg
     1741 gtcgcacgag aaagaaccta ggccgtgccc cggagggtcg caagatcccc tggggacggc
     1801 ggacgcgcgg tccgggcgca ccggcgcggg gcgcggccgc ccgggccgcg gacggggcgg
     1861 ggccggaccg gggagggatc gagggggctg gaccgccggg agggccgacg ccctccggcg
     1921 gtccccgtcg ccctcgtcag tgacgaccgg ccgggacgag ttcgcggcgc atgtagcggc
     1981 gctcctcggg ggtggtccca ccccagactc cgtgagcctc tcccgcccgc agggcccacc
     2041 gcaggcactc ttggcgaaca gtacagctgc ggcagacggc gatggcctgc tcggtgtcgg
     2101 cgcgcccgat cccggtgctg ctcaccggaa agaagatctc cggatcctgc gagcggcagt
     2161 tgccctgcag gacccagtcc tcgctctcgt ggtacaggcg gttcacaggc gcctccgatc
     2221 cgtggctggt ggtccctggc cccgtgccgc gtcaccacgg ccgggtcagg gggtttctcc
     2281 gccccaccgc ccacgggtga tcgacccggg aactgcggcg cggcgatgcg accaattgta
     2341 gtacgactca acgtagtact acacggagtc tgcgcgaaaa gaccggaaat ctcaagggcg
     2401 cctccggggt gcgggccgcc ccgcccgcgg gcgcgccccg ggtgggggcc ggggcccgac
     2461 tcaccccctg ccctcgcgcg gccgcgccaa aggccgcgtc ctcgcgcgtg tcggcgttgc
     2521 gggtgtgccg agagcccctt ccggcgtacc gtgtggcgcg ggcgcggagc cctcaagtgg
     2581 cgttatgtcg cgagctttcc accgaattga tgtgacttaa cgcacgctta atattgctcc
     2641 gcgaggatct atgtttgacc ctgacggttg tcggcccccc gagcgccggc gaccgccctc
     2701 cccggcggag ccccgagccc gctccccacg gccgcggcgc cgaaccccct gttgggcggg
     2761 gcgtgagcgc gtgtcccgtc cgcttccgga cgtcccccga aagtccaggt cagagctttg
     2821 gggcgccgct aaataaacag gccataccca taggcggtct gcccaaacat ccgcgagcgg
     2881 cctccacgaa atgtcattga cattcgttcg ctaaattcga tcgacgtgcc acatctacga
     2941 acgaacctcc aagcacgcga ccggactgac accggggcca cggccccgga gaatccaggc
     3001 gccgcgggcc gtggaggcgt gcgcctgtcg cacccgtccc cggaggacgg accgcacatg
     3061 tggcggctgg cgcgggagaa cggcatggac gccaactccc cgtacgccta cgcgctctgg
     3121 tgccgggact tcgccacgac cagcgtcgtc gcccgcgacg acgagggccg tgtcgcggcc
     3181 tacgtcaccg gctacgtgcg gcccgacgac cccgacacgt acttcctctg gcaggtcgcc
     3241 gtcgactccg cgtaccgcgg acaggggctg gcccgccgga tgctcgactt cgtcggcgac
     3301 cggatcaccg accgcggcct gcgctacctc gaggcgaccg tcaccgccga caacaccgcc
     3361 tcccgcgccc tgttcgcctc gttcgcccgg gaccgggacg ccgaggccgt gtggagtccg
     3421 ctcttcaccg gggaacactt ccctcaggag gacacccccc acgaccccga ggacctcgta
     3481 cgcatcggac ctctcggagc cgggcccgga gcatgaccga ccgtccgcac cggacccgtt
     3541 cgacccaccc cgtcgcccaa gccatgagcc cacgaacctg ggataaagga accgaggaac
     3601 acacgatgga gaccttccag cgcctggagt ccgaagtccg cggatactgc cggaactggc
     3661 cggtcgtgtt cgaccgggcc gtcggtagcc acgtctactc cgaggacggc aagccctacc
     3721 tcgacttctt cgcgggagcg gggtcgctca actacgggca caacaacccg gagctgaaga
     3781 cctcgctcat cgagtacctg accgacgaca agatcgtgca cagcctcgac gcctacagcg
     3841 tggccaaacg cgagttcctg aagaccttcg aggagatcat cctcaaaccc cggggcctcg
     3901 actacaaggt ccagttcccc ggccccgcgg gcaacaacgc ggtcgaggcc gcgctcaagc
     3961 tggcccgcaa gtacaccggt cgcgagacca tcgtcaactt caccaacggc ttccacggca
     4021 tgaccctggg agccctggcc gtcaccggca actcgatgaa gcgcggcggc gcgggcgtgc
     4081 cgctgggcca cgtcgccacg atgccgttcg acaactacct ggacggcaag acgccggact
     4141 tcctgtggct gcgcagcctg ctggacgaca gcggcagcgg cctggacaag cccgccgccg
     4201 tcatcgtcga gacggtccag ggcgagggcg gcatcaacgc cgccagcgcc cagtggctgc
     4261 gcgagctctc ggacctgtgc cgcgagtacg acatcctcat gatcgtcgac gacatccaga
     4321 tgggctgcgg ccgcaccggc gacttcttca gcttcgagga ggccgggatc atcccggaca
     4381 tcgtcacgct gtccaagtcc atcagcggct acggcctgcc catggccctc accctgttca
     4441 agcgcgagct ggacgtgtgg gagccgggcg agcacaacgg caccttccgc gggttcaacc
     4501 cggccatggt gaccgccgtc gaggccctgc gccgctactg gagcgactcg gccttcgccg
     4561 actccgtcaa ggccaagggc gacatggtcg ccgcccgcct ggccgagatg gccgccgagc
     4621 acgccgagtt cggcgcgcac gtgcgcggcc gcggcctggc ccggggcctg gccttcgagc
     4681 ggaccgacat cgccaagaag gtcgccgccg agtccttcga ccggggcctg ctcctggaga
     4741 cctccggccc cgaggacgag gtggccaaac tcctgccgcc gctcacggcg agcgaggagg
     4801 aactcacggt cggccttgac atcatggctg acgcggcccg cgccgcggtc aaggcggccc
     4861 agcccgccta gggactccca cagggggatt tccacaccac acggtgcggg gccgggcggc
     4921 gaccctcggg acgtcgtccg gcccctcccc gtgtgccgta ccaccacagg agcgctgaga
     4981 gcgcgtagag ggggagcaca gtgatcgttc gcagcatgga cgaggtcaac gacaccgacg
     5041 ccgacatcaa gacggagaac tggcgcagcc gtcggatcgt cctggccaag gacaaggtcg
     5101 gcttctcctt ccacgagacc gtcctgtacg cgggcaccga gtcgaccttc tggtacgcca
     5161 accacgtcga gctcgtgcac tgcatcgagg gcgaggccga gctgaccaac gaggagaccg
     5221 gggagaagca cctcatcacc ccgggcaccc tctacctgct cgacggccac gagaggcaca
     5281 ccgtccgccc caagacggac ttccgcgtgc tgtgcgtgtt caacccgccc gtcaccggtc
     5341 gtgaggtgca cgacgagaac ggcgtctacc ccctcgtcgt cgaggaagac gcctgaggcc
     5401 tgatcccagg actgtcagac cacgcgggcg gggccggtcg gccccgcccg ttcgcgttcg
     5461 cggaaggcgg cgtccgggcg tcctcggagt gtcgtgcggg tggcgcccca gggtgacgga
     5521 gaatcggacc caaagtcacg agcaccaggt gacccgccct gagcaggtca aaggccgggt
     5581 caccccgaga ctgcacgtga ataaccagtg cataacggac taatcgggtc ctgttactgc
     5641 cggtcctgcc cggggaggga gtcccccgta cttgtgccgc cgcgccctgt ccccgggcgg
     5701 cggccgcagc cgcggcccgg cgccgcgagc cgcccacgcc agtacctatc cgaatgcgcg
     5761 gcgctgtcgt ccagccaggc gagggcgccg ctcccgcgag tctttcgagg agcagtcgtg
     5821 aagaagccgg tacccgcagt acgcagagga acctggggcc gccgtgacgc actccgcctg
     5881 ggaggcgtgg ggctggccac cctcggactg gccgcctgca cccgcgtcga cggcggggga
     5941 gagggggagg agggcgcctc gaccctggac cggctgcgcg aacagggctt cgtggccgtc
     6001 ggcctcgcca acgagatccc cttcggcttc gtcgacgact ccggcaaccc ggccgggcag
     6061 tcccccgccg tggccaaggc ggtcttcgag gagctgggcg tccccgagat ccaggcctcc
     6121 tcggtgaact gggacggact gatccccggc ctgaacgcga accgcttcga cgtcatcgcc
     6181 gcgggcatgt tcatcacccc cgagcgctgc ggacaggtcg ccttcagcga gcccaccgcc
     6241 acctcccccg aggccttcat ggtggcggag ggcaacccgg agaacatcca gcacttcacg
     6301 gacttcgcgg acaaccccga cctcgtgctc gcggtcctca acggctcggt cgagcagacc
     6361 tacgccgagt acttcgaggt ccccgaggac cagatggagc tcatccccaa ccagaccgcg
     6421 ggctacgaac tgctgcaggc cgggcgcgtg gacgcgatgt ccatgctgag cgtgtcccac
     6481 acctacctcc tccaggaccg gggcggcccc ttcgaggtca ccgaaccctt cttccccgag
     6541 atcgacggcc gggaggagaa gggctggggc gggctgtgcc tgcgccagca ggacacccag
     6601 ctcctggagg aggtcaaccg ggtgatcgcc gagttcaagg agagcggacg cctcctggaa
     6661 ctgggcgagg agtggggctt caccgaggcc gacctgcccg acgacacgac caccgccgaa
     6721 ctctgcgagg gatgaacccg cagtggactt cctgatccag cggctaccgc tctacgccga
     6781 gggcgcgtgg gtcaccatcc agctcaccct cgggggcatc gtgctggcct tcgtcctcgc
     6841 catggtcttc gggatcatgg ggacgatcca ccactggctc ccccgggccg tcgccacggt
     6901 ctacgtggag gtgttccggg gcatcgccgc cctggtgctg atgttctgga tggcgttcgc
     6961 cctgccccag ctgaccggtt accagctggc cgacaacttc gccgcgatcc tcgccgtggg
     7021 gctcaacctg ggcgcctacg gggccgaggt cgtgcgctcg tccatcaacg ccgtgcccaa
     7081 gccgcaggtc gaggccaccg tcgcgttgaa cctctcctgg ttccagcgga cgcgcctggt
     7141 cgtgctgccc caggcctggg cccagatgct gcccacgttc ggcaacctca acatcgagtt
     7201 gatgaaggcc accgcggtgt cctacctcat cggtgtggcc gacctgacgg ccgtggcgca
     7261 gaacatgcgc caggccacgg gtgagacggt gatcgccttc atcggcgcct tcgtcctcta
     7321 ctacctgatc gcgcaggtgc tcatcctcgg catgcgactg ctcgaacgcc gcgccaaccg
     7381 caagctgggc cgcgccgcgc cgggcggcgg aggcctggcg tccctcctgc ggcccccggc
     7441 cgtcgacgcg aaggcggggg tggccaagtg atgttctggg acttcgagtg gaccttcgag
     7501 gtcatgcccg acctgctggg cggactgtgg gtgaccgtcc aggtcaccgt gctcggctac
     7561 ctgatcgcgc tggtcctggg cctggcgatc gccatcgctc gccggacgcc gttcttcggc
     7621 cccgtcctgc acgcggtcat ggagttcatc cggacgaccc cgctgatcat ccagctggtg
     7681 ttcgtcttcc tcctgacccc ggccggcgtc tccgccatga ccgtgggcgt cgtcgtgatc
     7741 ggcgtgcact acgcggccta caccgccgag gtctaccgct cgggtatcga gggcgtggcc
     7801 aaggggcagt gggaggcggc ccgggcgctg agcctgcccg ccagccgcac ctggggcgcg
     7861 atcgtgctgc cgcaggcgat ccgcaggacc atcccggcgc tgggcaacta cctcatcgcc
     7921 atgttcaagg acacgccgct gctgctggcg atcagcgtgc ccgagctgct caccgaggcc
     7981 cagcaggtcg gcagcgcgga cttccgcatc gtggaggcct acaccctggt cgggatcctg
     8041 ttcctgctcg tgagcatccc gtccgccatc ctcatccgac gactggagcg acgctatgcc
     8101 gccgtctgac acccccgtga acgaagaacc ccagggtgtc ggcagcggcc cccggaacca
     8161 gtccctgatc gtcttcgacg acgtggtgaa gcgcttcggc gacctcacgg tgctcgacca
     8221 cctgaacttc tccgtcgccc ccggcgagcg ggtcacgctc atcggcccca gcggttcggg
     8281 caagaccacc atcctgcgcc tgctgatgac cctggagaag gtcaccgagg gcgtcatctg
     8341 ggtgggcggc gagcccttca gccacatgcg caaggggcag ggcctgaccc gggcgaacga
     8401 gtcctacctg cgcgcgcgcc gcaagcgcgt cggcatggtg ttccagcagt tcaacctgtt
     8461 cccgaacatg accgtccggc agaacgtcac cgaggcgccc gtgcacgtgc tgggcactcc
     8521 caagggcgag gccaacgcca aggccgagga actgctggag ctggtcgggc tcggcgacaa
     8581 gatcgacgcc cacccgaccc agctctcggg cggccagcag cagcgcgtgg cgatcgcccg
     8641 cgcgctggcc atgcagccgg agatcctgct cctggacgag gtcacctcgg ccctggaccc
     8701 ggagctggtg gccggggtcc tggacgtgct gcgcgaggtc gccgagacca cggacatcac
     8761 gatgctgtgc gtgacgcacg agatgggctt cgcgcgcgac gtgtcgcacc gggtgctcat
     8821 gttcgaccag ggccagatcg ccgaggaggg cacgccggag cagatcttcg gcgaccccaa
     8881 ggaggagcgc accaagtcct tcctccgctc ggtgctcgac aacggtttct gaagggcccc
     8941 cgaacccgcg taccgccggg gccgctccct ggggagcggc ccctcggcgt gacgggacat
     9001 gtcgaggcga gtggcgacat accgtagtca ttaagtagct tttagtttcc ccttgggcag
     9061 aatgagagtt ctgagacagg ggggactctt gtgaccatgg tgcgaagatg ggggctcgtc
     9121 tggggggtgc tggccgccct ggtgttcgtc acggtgccga tgctcgtcca ccccgtactc
     9181 cgctccgacg tcctcgacga ctccctggtc ctcttcctga tctcctggtc cccgatgctg
     9241 atcggcggca tcctggcctg ggccatggtc aggggcttcg gcgcccgcga ccagctggac
     9301 cgccgcgtcg tccaggcgat ggacggccac cccatcggcc gggaactggg ctggctggga
     9361 ctgttcctcg ccggcttcgt ggtgtccatg gcgctgctgt cgatggtctt cgccacctac
     9421 gaggccgtgt tcggggccga cctggccatg tccgaggccc tggtctcgcg cctgctgttc
     9481 ctgttcaccc tgccgctgct ggtcatggac cgctccggca tcaccctgga cggcaagggc
     9541 accgccatgc ccgcggtcgc gctgaaggtg tccgagccct ggcgctggct cgggatggtc
     9601 ccgatcgcca tcgcgctggt cctgatcggc tacctgctca tcccgcgcct ggggctgccg
     9661 gggccctccc tccaactgct gggctacgtg ctcgcgttca ccgtcatggc ggtgtgcgag
     9721 gagatcttct tccgggggat ggtgcagacc cgcctggaga tcctcatggg ccgctggggc
     9781 ggcatcgtca ccacctcggt ggtcttcgcc ctcacctacg ccctcatcca gccctacgac
     9841 gcggtgtccc agctgccggg ggccgacctg gtgtacaaca cgggcctggc cctgctcacc
     9901 tacgccacgg ccagcctcct ctacggctac ctgtggacgt gcttccgcaa cacgtggatc
     9961 caggtggtga tgcgcgtcgg catgttcctg atgctcatgc cccccgatct ccagaccggc
    10021 gtcatgtgac cggacccgac caccggacga acggtaaaac ccgcggtcgg aaggggaaaa
    10081 ccgcgtcggg aacgggcata gggctacaga gggtccacac cagtcgtgtc gcggtcattt
    10141 tcccctcacg gggttgtcac cttgcggtca tggaaaaggg ggattctgcc cactgtgagc
    10201 acggaaacag cgccaacacc cgaggggccc gccgccggta cgcccgagct tcccgccgac
    10261 aggttcatgg acagggagga ggggtggctg cgcttcaacc agcgcgtgct cgaactcgcc
    10321 gaggacgagg acgtccccct cctggaacgc gcgcggttcc tggcgatctt ctccagcaac
    10381 ctggacgagt tcttca
//
