LOCUS       NZ_JAMXJO010000001     10873 bp    DNA     linear   CON 29-AUG-2024
DEFINITION  Nocardiopsis dassonvillei strain RACA_4 assembly, whole genome
            shotgun sequence.
ACCESSION   NZ_JAMXJO010000001
VERSION     NZ_JAMXJO010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nocardiopsis dassonvillei
  ORGANISM  Nocardiopsis dassonvillei
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsaceae; Nocardiopsis.
REFERENCE   1  (bases 1 to 6726007)
  AUTHORS   Elfeky,H.H., Hanora,A. and Solyman,S.M.
  TITLE     Bioactivity of bacteria associated with Red Sea nudibranchs and
            whole genome sequence of Nocardiopsis dassonvillei RACA-4
  JOURNAL   Mar Genomics 67, 101004 (2023)
   PUBMED   36521348
REFERENCE   2  (bases 1 to 6726007)
  AUTHORS   Elfeky,H., Hanora,A.S. and Solyman,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2022) Microbiology, Suez Canal University, 4 Km
            Circular Road, Ismailia 41511, Egypt
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Assembly Name                     :: SCU_Nocdas_4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: GCA_900638215.1
            Genome Coverage                   :: 7.6x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024181525.1-RS_2024_08_29
            Annotation Date                   :: 08/29/2024 06:43:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,838
            CDSs (total)                      :: 5,775
            Genes (coding)                    :: 5,721
            CDSs (with protein)               :: 5,721
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 7 of 54
            Pseudo Genes (incomplete)         :: 51 of 54
            Pseudo Genes (internal stop)      :: 0 of 54
            Pseudo Genes (multiple problems)  :: 4 of 54
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:23:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2630916
            Orig. end                         :: 2641789
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAMXJO010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10873
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="phosphonate"
                     /region_number="11"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     cand_cluster    1..10873
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10873
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5873](-)"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5873)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     misc_feature    175..184
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            <197..>596
                     /locus_tag="NGM33_RS11255"
                     /old_locus_tag="NGM33_11255"
                     /pseudo=""
     CDS             197..>596
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017571116.1"
                     /locus_tag="NGM33_RS11255"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="NGM33_11255"
                     /product="zinc ribbon domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTNHQISKRIVAEAERTGRGISPEDLTRIRIRVRQRRPQRVTLHT
                     WSFHHLGQFIVCKARRTGVPVVFVDAAYTSQTCAGCGHVDHRNRVDQATFLCRGCGVVA
                     HADRNASADIAARGRVVRNAGRKSHVPPD"
     assembly_gap    597..696
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="clone contig"
     gene            896..1531
                     /locus_tag="NGM33_RS11260"
                     /old_locus_tag="NGM33_11260"
     CDS             896..1531
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019611198.1"
                     /locus_tag="NGM33_RS11260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_11260"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_130394854.1"
                     /transl_table=11
                     /translation="MTERRREIVRTEIARAAVRLFTENGVSGTTGDDIAHALGISTRTL
                     WRYFPSKESCVRPLLTTGLEHMAERLRACPPGVPLLEYLERERFFDGEPQVVEGPVADL
                     IRMTRTEPALMAVWLEVHHSAESVFAGIIADRTGEPADSLPVRVQAATLNSALRLAAEE
                     RARLGNDGRGLPPEGVGPLLRTALTTAARGLPAFGHGGGDERGEAPPD"
     gene            complement(1723..3057)
                     /locus_tag="NGM33_RS11265"
                     /old_locus_tag="NGM33_11265"
     CDS             complement(1723..3057)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1074: major
                     facilitator family transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019611199.1"
                     /locus_tag="NGM33_RS11265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_11265"
                     /product="MFS transporter"
                     /protein_id="WP_253794725.1"
                     /transl_table=11
                     /translation="MSSPDSAGSSPAPTPVGAPVRDRRTALRAAVAGGVGTLIEYYAFS
                     VYGFLTVVIAPLFFPSSNPATSVLATLAVFGSGYLMRPLGGIFFGRLGDRTGRKVALVS
                     TVVSMGVCCALMGMLPTYPQAGIIAPILLVVVRMAEGFSAGGEVGGSATYIAESSPAGM
                     RGFFGAFTPTGSTLGFAVAAAVTGAVVGLLAPEQMTAWGWRIPFLLCVPLTLLCLWARL
                     KLEDTPDFREMETKAEVARSPFTDVLRRNPGAVLKVVGLAIATNGTGYVGLTYMNVYLI
                     NDLGHDSQDVYWLSAAVIAAACACMPFLGLLADRFGRLRVLTAGCVGYVLLAYPILLVM
                     SQASGLLVIGLMYLVFMVLNATLQVPAFPLFTELFPRAVRYTGVALGFNIGTIVAGGTA
                     PYIAAQLVASTGDPVSPAYWVMGVAVVGLVTAFTVRSTRDGELPA"
     gene            complement(3200..3733)
                     /locus_tag="NGM33_RS11270"
                     /old_locus_tag="NGM33_11270"
     CDS             complement(3200..3733)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019611200.1"
                     /locus_tag="NGM33_RS11270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_11270"
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="WP_130394855.1"
                     /transl_table=11
                     /translation="MSPLPPPDVIRGRVAWIFGDDFDVDLIIGVENIKYYDPDVLRSVC
                     MRHHDPAFVDEVRPGDVVVGGRNFGYGHPHYPPLVALRNLGVTAVIAESFSPGFWRGET
                     YNGMPLLTVPGITRAVRRWDRVEVDWRRPSVRLPDRDAELTGNPLTERVVKVIEAGGSL
                     NLLLSEHRSTHTAG"
     gene            complement(3730..4998)
                     /locus_tag="NGM33_RS11275"
                     /old_locus_tag="NGM33_11275"
     CDS             complement(3730..4998)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019611201.1"
                     /locus_tag="NGM33_RS11275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_11275"
                     /product="aconitase family protein"
                     /protein_id="WP_253797500.1"
                     /transl_table=11
                     /translation="MGLTITEKILGRAAGGATVRARDNRAFRPDHMIAYDFPGYTDVMF
                     RQMHDDFGITELADPERYLVFIDHMLTRGSAKEAEVHQVTRDWCDFYGITLHEAEGIGH
                     QVAAELGYALPGRFLIHFDGHISGLGAFGALGWGVRRDLLEAWVTGQIFLDVPESVRFE
                     LTGAFPEGVDNRDLIHAIIARLGADGCAHQVMEFTGPGARSMSIDRRQGLCGMAMFTGA
                     VSAIFNPDELSLEYARTVSDTVPEPLYSDPDAVYAGVHEIDLSELEPQVVLPGSARGQN
                     TRGVSELEGTPVTKAFIGSCASGRLEDIRAAAGVLRGRKVADGVQLNVVPTSAAIHRQA
                     EAEGLLDVLRDAGAAVAESSCDFCFGYQKPLEAGENCVSTGVLNISGRMGSTDANIYMG
                     SAYTVAASAVTGRICDVREVTGP"
     gene            complement(5001..5873)
                     /locus_tag="NGM33_RS11280"
                     /old_locus_tag="NGM33_11280"
     CDS             complement(5001..5873)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231: carboxyvinyl-carboxyphosphonate"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019611202.1"
                     /locus_tag="NGM33_RS11280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_11280"
                     /product="oxaloacetate decarboxylase"
                     /protein_id="WP_130394856.1"
                     /sec_met_domain="phosphonates (E-value: 8e-45, bitscore:
                     151.4, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 1.5e-48, bitscore:
                     163.7, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MESQRLRELMDAGVVYAPGVWDGLTARLAEQAGFPALCASGFAVS
                     ASLGYPDAELYTMTENLDATRRIREASGLPLIADIDTGYGNAVNAARAGRRFRDAGVAA
                     VFMEDQESPKRCPLCVGDPVPLVPTQEGAGKVRAVKDAVGDDVLVIARTDAAGDDALRR
                     AEAYVGAGADMIMPVTRTFSTVDEWARCREAAGVPLVATLTASTWTEREFTPRVLEEVG
                     VALALLPTQVLMAATGAMRRALGRLAGGEPPAQVSADALQHHEFVDLIGFAEVEAAQRD
                     YLPAAQGGE"
     misc_feature    complement(5861..5879)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 18.92"
                     /tool="antismash"
     misc_feature    complement(6006..6021)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.19"
                     /tool="antismash"
     gene            6101..7711
                     /locus_tag="NGM33_RS11285"
                     /old_locus_tag="NGM33_11285"
     CDS             6101..7711
                     /EC_number="6.2.1.3"
                     /NRPS_PKS="Domain: AMP-binding (25-438). E-value: 6.2e-75.
                     Score: 244.2. Matches aSDomain:
                     nrpspksdomains_NGM33_RS11285_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /locus_tag="NGM33_RS11285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_11285"
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="WP_253794727.1"
                     /sec_met_domain="AMP-binding (E-value: 2.8e-82, bitscore:
                     274.9, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQTEPLGLHQVFDRAETTFADRGITGREADGVRTLDYAGLFAGAR
                     GLARILTGLGLRPGDRVATYAWNTLDHLRAYVAIPGSGMVMHTVNVRLSADEIAYTLGH
                     AGDRAVLVDGSLWEAWSAVDLPDTVEHVLVMGEAPELPSSQGGARVRRLELPAAPDQRS
                     WWRPRDENEAAGVCYTSGTTGRPKAVVYSHRSVYLHALTLCTTDAFAIGHHDVVLPVVP
                     MFHANSWNLPYAALVSGAGLALPGRHLDPLSLAGFVRDAGATFAAGVPTVWSSLVRAVE
                     EGRVPADALATLDRVVIGGSSASENLLDALTGLGVRPVHAWGMTECSPVGLVAVEPCVP
                     DEAARAAARRTQGRPLPGLAVRSTAAGGEVPRDGSSVGELEISGPFVVDRYHAPEQDDG
                     ASFDSSPDGRRWLRTGDMVTVDARGYVTLVDRAKDMVKSGGEWISSVDLENRLAAHPRV
                     REAAVIAVPDPTWGERPVAVLSSTDGGEIAADELRGFLGEGFARWQLPDRFVMVEEVPK
                     TSVGKFDKKRMRAVYGSES"
     aSDomain        6176..7414
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NGM33_RS11285_AMP-binding.1"
                     /evalue="6.20E-75"
                     /label="NGM33_RS11285_AMP-binding.1"
                     /locus_tag="NGM33_RS11285"
                     /protein_end="438"
                     /protein_start="25"
                     /score="244.2"
                     /specificity="substrate consensus: Asp"
                     /tool="antismash"
                     /translation="GREADGVRTLDYAGLFAGARGLARILTGLGLRPGDRVATYAWNTL
                     DHLRAYVAIPGSGMVMHTVNVRLSADEIAYTLGHAGDRAVLVDGSLWEAWSAVDLPDTV
                     EHVLVMGEAPELPSSQGGARVRRLELPAAPDQRSWWRPRDENEAAGVCYTSGTTGRPKA
                     VVYSHRSVYLHALTLCTTDAFAIGHHDVVLPVVPMFHANSWNLPYAALVSGAGLALPGR
                     HLDPLSLAGFVRDAGATFAAGVPTVWSSLVRAVEEGRVPADALATLDRVVIGGSSASEN
                     LLDALTGLGVRPVHAWGMTECSPVGLVAVEPCVPDEAARAAARRTQGRPLPGLAVRSTA
                     AGGEVPRDGSSVGELEISGPFVVDRYHAPEQDDGASFDSSPDGRRWLRTGDMVTVDARG
                     YVTLVDRAKDMVKS"
     CDS_motif       6626..6679
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NGM33_RS11285_0001"
                     /evalue="6.50E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="NGM33_RS11285"
                     /protein_end="193"
                     /protein_start="175"
                     /score="21.8"
                     /tool="antismash"
                     /translation="VCYTSGTTGRPKAVVYSH"
     gene            7822..8646
                     /locus_tag="NGM33_RS11290"
                     /old_locus_tag="NGM33_11290"
     CDS             7822..8646
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1195: IclR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019611204.1"
                     /locus_tag="NGM33_RS11290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_11290"
                     /product="IclR family transcriptional regulator"
                     /protein_id="WP_253794729.1"
                     /transl_table=11
                     /translation="MATIDGEPAVGPKGVVNSVLRACRLLECFTHERHALTLGELAEET
                     GLNKTTVHRLLATLVQVGWVSQRADGAYKVNMRLFAVGSTALAELNLRDEAAPLLRGLA
                     ARTGHTAYLMVPSEDGAVCIDLVEGSGPLTIKKIAVGSVLPFHAAAGPMTMLAFSADLR
                     ERWLARPLRSFTAHTLTDGHSLRTHLERIASEGYTVSDEDFLDDVGAVAAPVFDSSGGL
                     AATVSLGGPATHFRGSGLVGLIAAVREAAAELSVQLGARPRSGLPEAGPPVR"
     misc_feature    9152..9166
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.34"
                     /tool="antismash"
ORIGIN
        1 gttagcaaca ggggacacat cggaccgcct tggctgcgtt ctgtcaccgg ccgggtgcgc
       61 gatggtgggc gtgtcgaaga tcgtcgcgtg ggtcaagccc acgccgtcgc ccgagcaggc
      121 ggcggtgctg gcctcgaccc tgggcgcact caacgaccac gccgcctggg tggcgaaggc
      181 ggcccacgag cagggcaaca ccaatcacca gatctcgaag cgcatcgtgg ccgaggccga
      241 acgcaccggg cgcggtatct cccccgagga tttgacgcgg atacgcatcc gggtacggca
      301 acgcaggccc caacgggtca cgctgcacac ctggtccttc caccacctgg gtcagttcat
      361 cgtctgcaag gcgcgccgca cgggggtgcc ggtggtcttc gtcgatgcgg cgtacacctc
      421 acagacgtgc gctgggtgtg gccacgtgga ccacaggaac cgggtcgatc aggccacctt
      481 cctctgccgg ggctgcggtg tcgttgcaca cgcggaccgc aatgcttccg ccgacatcgc
      541 cgctcgcggt cgtgtggtgc ggaacgcggg gcggaagtca cacgtcccgc ccgaccnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcgg agtggtcagg tcagacgcgg
      721 tgggctcctc cgcatccagc ggggtggtcc gaccacacgc tcgatccctt cgggaccgag
      781 aagctgacta gagtggatcc gcccaggcag cgcgcacacg ggagggggga cggtcgtgcc
      841 gggaccaccg tccgcgaaga cgccccgcgc accctcgggg cggggccgcc cgcccatgac
      901 cgagcggcgc agggagatcg tgcgcacgga gatcgcgcgc gcggccgtgc ggctgttcac
      961 cgagaacggg gtgtcgggca ccacgggcga cgacatcgcg cacgcgctcg gcatctccac
     1021 caggacgctg tggcgctact tccccagcaa ggagagctgc gtccggccgc tgctgacgac
     1081 gggcctggag cacatggccg aacgcctgcg cgcctgccct cccggggtcc ccctcctgga
     1141 gtacctggag cgggagcggt tcttcgacgg cgagccgcag gtcgtggagg ggcccgtagc
     1201 agacctcatc cggatgaccc gtaccgaacc ggcgctcatg gcggtgtggc tggaggtcca
     1261 ccactccgcc gagtccgtct tcgccgggat catcgccgac cgcaccggcg agcccgccga
     1321 cagcctgccc gtccgcgtcc aggcggccac cctcaactcg gcgctgcgcc tggccgccga
     1381 ggagagggcg cgcctgggga acgacgggcg cggccttccg ccggagggcg tggggccgct
     1441 gctgcgcacc gcgctgacca ccgcggcccg cggcctgccc gccttcgggc acggcggcgg
     1501 ggacgagcgc ggggaggcgc ccccggactg agtcccctcc ggccgccccg tgcgcggggc
     1561 ccgcgcacgg gggagcgctg gaccgcgaag gcggccccat acgcgggggc ccgcgggtgc
     1621 cgcggaaccg tctcgtcctc cggccgccgt gcgcgggggc ccgcgaccgg acggggcggc
     1681 gctgggggct ccggcccggc gggcgcaggg ccggagccgg gatcaggccg ggagctcccc
     1741 gtcgcgggtg gagcggaccg tgaacgccgt gaccaggccg accaccgcca cccccatcac
     1801 ccagtacgcc ggggacaccg ggtcgcccgt cgaggcgacc agctgggccg cgatgtaggg
     1861 ggcggtcccg ccggcgacga tcgtgccgat gttgaagccc agggcgaccc cggtgtagcg
     1921 cacggccctc gggaagagtt cggtgaacag cgggaacgcc gggacctgga gcgtcgcgtt
     1981 gaggaccatg aacaccaggt acatcagccc gatgaccagc agccccgagg cctgcgacat
     2041 gacgagcagg atggggtagg ccaggaggac gtacccgacg caccccgcgg tgagcaccct
     2101 caggcgtccg aagcggtcgg cgagcaggcc caggaacggc atgcacgcgc aggccgcggc
     2161 gatcacggcg gccgagagcc agtacacgtc ctgggagtcg tggcccaggt cgttgatcag
     2221 gtacacgttc atgtaggtca gcccgacgta ccccgtgccg ttggtggcga tggccaggcc
     2281 gacgaccttg aggacggcgc ccgggttgcg ccgcagcacg tcggtgaagg ggctgcgggc
     2341 cacctccgcc ttggtctcca tctccctgaa gtcgggggtg tcctccagct tcagccgcgc
     2401 ccacaggcac agcagggtca gcgggacgca cagcaggaac gggattcgcc acccccaggc
     2461 cgtcatctgc tcgggggcga ggagtccgac gacggcgccg gtcacggcgg ccgcgaccgc
     2521 gaagccgagc gtggagccgg tcggggtgaa cgcgccgaag aagccgcgca tgccggccgg
     2581 ggaggactcc gcgatgtagg tggccgagcc gcccacctcg cctccggcgg agaagccctc
     2641 ggccatgcgc acgaccacca gcaggatcgg ggcgatgatc ccggcctgcg ggtaggtggg
     2701 cagcatgccc atcagggcgc agcacacgcc catgctgacg accgtggaga ccagcgcgac
     2761 cttgcgtccg gtccggtcgc cgaggcggcc gaagaagatc ccgccgagcg gccgcatgag
     2821 gtagccggac ccgaacacgg cgagggtggc cagcaccgag gtggcggggt tggaactggg
     2881 gaagaacagc ggggcgatga cgacggtcag gaaaccgtag acgctgaacg cgtagtactc
     2941 gatcagggtc cccaccccgc cggcgaccgc ggctcgcaag gcggtgcgcc ggtcgcgcac
     3001 gggtgcgccg acgggggtcg gtgccgggga gctcccggcg ctgtctggag aggacaaggc
     3061 gatgcctcct ggtgaggacg ggacgtgggc acccgtcagg ggtccacggg gaagggcgcc
     3121 tccccgtggg ccggggcggc gggacgggac gccggtccgg cgctcccgcg tgtcggtgcg
     3181 gcggtcctga cctggccggt cagcccgcgg tgtgtgtcga gcggtgctcg gacaggagca
     3241 ggttcaggct ccctcccgcc tcgatgacct tgaccacgcg ctcggtgagc gggtttccgg
     3301 tcagctccgc gtcccggtcg ggcaggcgta cggacgggcg ccgccagtcg acctcgacgc
     3361 ggtcccaccg ccggacggcg cgggtgatgc ccgggacggt cagcagcggc atgccgttgt
     3421 aggtctcccc gcgccagaag ccgggtgaga aggactcggc gatcacggcc gtcaccccca
     3481 ggttgcgcag cgcgacgagc ggggggtagt gcgggtgccc gtagccgaag ttgcggcccc
     3541 cgacgacgac gtcgccgggg cgcacctcgt cgacgaacgc ggggtcgtgg tgccgcatgc
     3601 acacgctccg cagcacgtcg gggtcgtagt acttgatgtt ctccacgccg atgatgaggt
     3661 cgacgtcgaa gtcgtccccg aagatccagg cgacgcgtcc cctgatgacg tcgggcggcg
     3721 gaagcggact catggcccgg tcacctcccg cacgtcgcag atccgcccgg tcacggcgct
     3781 ggcggcgacg gtgtaggcgg agcccatgta gatgttggcg tcggtgctgc ccatgcgccc
     3841 ggagatgttg agcacgcccg tggacacgca gttctccccg gcctccaggg gtttctggta
     3901 accgaagcag aagtcgcagc tggactcggc cacggccgct ccggcgtcgc gcagcacgtc
     3961 cagcagtccc tccgcctcgg cctggcggtg gatcgccgcc gaggtcggga cgacgttgag
     4021 ctgcacgccg tcggccactt tacggccccg cagcaccccg gcggcggccc ggatgtcctc
     4081 cagcctcccg ctggcgcagg agccgatgaa cgccttggtg acgggggtgc cctccagctc
     4141 cgagaccccc cgcgtgttct ggcccctggc cgacccgggc agcacgacct gcggctccag
     4201 ctccgagagg tcgatctcgt gcacaccggc gtagaccgcg tcggggtcgc tgtacagcgg
     4261 ctcgggaacg gtgtcggaca ccgtgcgggc gtactccagc gagagttcgt ccgggttgaa
     4321 gatcgccgac accgcgccgg tgaacatcgc catcccgcac aggccctgcc tgcggtcgat
     4381 gctcatcgag cgcgctccgg ggccggtgaa ctccatgacc tggtgggcgc agccgtcggc
     4441 gccgagccgg gcgatgatgg cgtggatgag gtcgcggttg tccacgccct cggggaaggc
     4501 cccggtgagc tcgaagcgca ccgactccgg cacgtccagg aagatctgac cggtgaccca
     4561 cgcctccagg aggtcgcgcc gcacccccca cccgagcgcc ccgaaggccc ccaggccgct
     4621 gatgtggccg tcgaagtgga tgaggaaccg gcccgggagg gcgtagccga gttcggcggc
     4681 gacctggtgg ccgatcccct cggcctcgtg cagggtgatc ccgtagaagt cgcaccagtc
     4741 gcgggtgacc tggtggacct ccgcctcctt ggcactgccc cgcgtgagca tgtggtcgat
     4801 gaagaccagg tagcgctcgg ggtcggccag ttccgtgatg ccgaagtcgt cgtgcatctg
     4861 gcggaacatc acgtcggtgt agccggggaa gtcgtaggcg atcatgtggt cgggccggaa
     4921 cgcgcggttg tcgcgggcgc ggaccgtggc tcccccggcg gcccggccga ggatcttctc
     4981 ggtgatggtc aggcccatgc tcactcgccc ccctgcgcgg cgggcaggta gtcccgctgc
     5041 gcggcctcga cctcggcgaa cccgatcagg tcgacgaact cgtggtgctg gagcgcgtcg
     5101 gcgctcacct gggcgggggg ctctcccccg gccagcctgc ccagggcccg gcgcatggcc
     5161 cccgtcgccg ccatcagcac ctgggtgggc aggagcgcga gcgctacccc gacctcctcc
     5221 agtacccggg gggtgaactc gcgctcggtc caggtggacg cggtgagcgt ggcgacgagg
     5281 gggacccccg ccgcctcccg gcacctcgcc cactcgtcga cggtggagaa ggtcctggtg
     5341 accggcatga tcatgtcggc cccggcgccg acgtaggcct cggcgcggcg cagcgcgtcg
     5401 tcgcccgcgg cgtcggtgcg cgcgatgacg aggacgtcgt cgccgacggc gtccttgacc
     5461 gcgcgtacct tccccgcccc ctcctgcgtc gggaccaggg gcaccgggtc gccgacgcag
     5521 aggggacagc gcttgggcga ctcctggtcc tccatgaaga ccgcggcgac cccggcgtcg
     5581 cggaaacggc ggcccgcccg cgcggcgttg acggcgttgc cgtacccggt gtcgatgtcg
     5641 gcgatcagcg gaaggccgct cgcctcgcgg atccggcggg tcgcgtcgag gttctccgtc
     5701 atcgtgtaca gctcggcgtc cgggtagccg agggaggcgg agacggcgaa gcccgaggcg
     5761 cacagggccg ggaacccggc ctgttcagcc aggcgcgcgg tgagcccgtc ccagaccccc
     5821 ggcgcgtaga ccactcccgc gtccatcagc tcccgtagtc gctgggattc cataccgcct
     5881 gcctttcgta tagcggaacg cgtttcaaaa ggaggctaag gaggcgtatg atcctgtgtc
     5941 aaggatcact tcgttacgta actcagccga cactcgacgg gtctacatgc tggaacgcca
     6001 acataccgac caggcggtcc gtgcacgggt ggccccgtgt ccacaccaca cggaacacgt
     6061 ttcgcacaga acgggagagt gaaccgcgat gatctccgtg atgcagaccg aaccgctggg
     6121 actgcaccag gtcttcgacc gggccgagac gaccttcgcc gaccgcggga tcaccggccg
     6181 ggaggcggac ggcgtccgca cgctcgacta cgccgggctg ttcgccgggg cccgcggact
     6241 cgcgcggatc ctgaccggcc tcgggctccg gcccggcgac cgggtggcca cctacgcctg
     6301 gaacaccctg gaccacctgc gcgcctacgt cgcgatcccc ggctcgggca tggtcatgca
     6361 caccgtgaac gtccgcctca gcgccgacga gatcgcctac acgctcgggc acgccgggga
     6421 ccgcgcggtg ctcgtggacg ggtccctgtg ggaggcctgg tccgccgtcg acctccccga
     6481 caccgtcgag cacgtcctcg tcatgggcga ggcccccgaa ctcccctcct cccagggcgg
     6541 agcgcgcgtg cgccgactgg aactccccgc ggcccccgac cagcggagct ggtggcgccc
     6601 ccgcgacgag aacgaggcgg cgggcgtgtg ttacacgtcg ggcaccaccg gacgccccaa
     6661 ggcggtggtc tacagccacc ggtcggtcta cctgcacgcg ctcaccctgt gcacgaccga
     6721 cgcgttcgcc atcggccacc acgacgtggt cctgccggtc gtgccgatgt tccacgcgaa
     6781 ctcgtggaac ctgccctacg cggccctggt gtcgggcgcc ggtctggccc tgcccggccg
     6841 ccacctggac ccgctcagcc tcgcgggctt cgtgcgcgac gccggggcga cgttcgccgc
     6901 cggggtgccc accgtgtggt cctccctggt ccgggcggtg gaggagggcc gggtgcccgc
     6961 cgacgcgctg gcgaccctgg accgggtcgt catcggcggc tcctccgcct ccgagaacct
     7021 gctcgacgcg ctcaccggcc tcggggtccg ccccgtccac gcctggggca tgaccgagtg
     7081 ctcaccggtc ggtctggtcg ccgtggaacc gtgcgtcccc gacgaggcgg cgcgcgcggc
     7141 ggcccgccgc acgcagggcc gccccctgcc cggcctggcg gtccggtcga cggccgccgg
     7201 aggggaggtc cccagggacg gctcctccgt gggggagctg gagatcagcg gccccttcgt
     7261 cgtggaccgc taccacgccc ccgagcagga cgacggcgcc tccttcgact cctcccccga
     7321 cgggcgccgc tggctgcgca ccggggacat ggtcaccgtc gacgcgcggg ggtacgtgac
     7381 cctggtcgac cgcgccaagg acatggtcaa gtccggcggg gagtggatct cctcggtgga
     7441 cctggagaac aggctggctg cccacccgcg ggtgagggag gcggccgtca tcgcggtccc
     7501 cgacccgacc tggggcgagc ggcccgtcgc ggtcctgtcc agcacggacg gcggagagat
     7561 cgccgcggac gagctgcgcg gcttcctggg ggaggggttc gcgcgctggc agctccccga
     7621 ccggttcgtg atggtggagg aggtccccaa gaccagcgtg ggcaagttcg acaagaagcg
     7681 gatgcgcgcg gtgtacggca gtgagagctg aaccggacgg cggcgccgcg cccgccggag
     7741 ccgggtccac ggcgtcggga ggcacgcggt ccggccgcta ccctgacacc gacggccgaa
     7801 cgtgcgagga ggtccccttc ggtggcgacg atcgacggtg aaccggccgt gggacccaag
     7861 ggcgtggtca actcggtgct ccgcgcctgt cggctcctgg agtgcttcac ccacgagcgg
     7921 cacgcgctca ccctgggcga gctggccgag gagaccggcc tgaacaagac cacggtgcac
     7981 cgcctgctgg ccacgctggt ccaggtcggc tgggtcagcc agcgcgcgga cggcgcctac
     8041 aaggtcaaca tgcggctgtt cgccgtcgga tcgaccgcgc tcgccgaact caaccttcgc
     8101 gacgaggccg ctcccctgct gcgcggcctg gccgcgcgca cgggccacac ggcctacctg
     8161 atggtgccct cggaggacgg cgcggtctgc atcgacctcg tcgagggctc ggggccgctg
     8221 acgatcaaga agatcgccgt cggcagcgtg ctgcccttcc acgccgcggc ggggccgatg
     8281 acgatgctgg ccttctccgc cgacctgcgg gaacggtggc tggcccggcc gctgcggagc
     8341 ttcaccgcgc acaccctgac cgacgggcac tccctgcgca cgcacctgga acggatcgcg
     8401 tccgagggct acaccgtcag cgacgaggac ttcctggacg acgtgggcgc cgtggccgcc
     8461 cccgtcttcg actcctccgg ggggctcgcc gccacggtga gcctgggcgg ccccgcgacg
     8521 cacttccggg ggtcgggcct ggtcggcctc atcgcggccg tccgcgaggc ggcggccgaa
     8581 ctgtcggtcc agctgggtgc ccggccccgg agcggcctgc cggaggccgg gccccccgtc
     8641 cggtaggagg cggcggtctg cccgccgcct cccgccgatc cgcccgcccc ggcttgcgcc
     8701 ggggcgggtc ctcccgccgg ggccgcccgg tcggcgtctc ctcccgcgcc cgtgcccccg
     8761 cccccctact ccgggaagac ggcttcgggg acctccaccc gcgcccagtc ggtgtccgcg
     8821 cccaggtgga aggacgtgta cgcgggctgg ttgtagccgg tctgctgccg ggccacgtcc
     8881 acccggtact gcgggtcgtg catgagggtg tgcagccggt gtccggtcac ctcggtgctg
     8941 gtgaggatgc gcagcgcgcc gctgtcctcc gtgcgcagga ccagctcctc gcgccagtcc
     9001 ccgagcacgt ccgcgaccag ggcggggttg cccttggtcc cgttgttggt gagcgtgccc
     9061 tgggcggtga gcagcgtgcc gcgccgccag tcctcgatcc tcgggtcctc ggtcccctcg
     9121 acgatctgcg tggtcatgtc ggcggaccac ctgatgctca tgttcgtgcc gggggtgcgg
     9181 tcgcccagga actcgccccc ggcgctccac aggcccgcct cgcccccgcc gccgggcggc
     9241 atcgaggacc acgcctccag gcccgggacg tccgggtcga cgtcgccgac catgccccgg
     9301 ccggtgtcgg tgcccgtgta cccgccgaac agcacctcgc cggtggcggc gtcgcgcatc
     9361 gcgaagccgt acggcgcgct cgcgccgccc tcgtgcacgg tccagacctc caggccctcg
     9421 cgctccgggt cgaggtcgcc cacgtgcatc gcgtcgccgt ggccgagctt ggtcgtctgg
     9481 ccctcgacgg cgctcccggg cgggccctcg gcgtgcgagg agtacagcag cgacccgtcg
     9541 tcgtccaggg tcgcggagcc gtagacgatc tcctggcgtc catcggcgtc cacgtcggcc
     9601 acgccgaagg agtggaagcc ctggcccgcg agcgttccca tgtcggggtc ggagccccgg
     9661 ccgccgtgcg gggaggcgtc gaacgggttg tccatgggca cgtgcccgct gtccgcgatc
     9721 cagcgcaggc tgatgtcccg cccgtcgaag tcgtaggcgg ccacggccga gcgcgtgtag
     9781 tagccccggg cgaacaccgc cgacgggcgg gtgccgtcca ggtaggccac gcccgacagc
     9841 agccggtcca cccggttggc gggttcgatg cgggccatcg cgtagtcgcc ccacatcagg
     9901 ccgtcgtcgc cccggcccgg ctcgtagcgg acggtgtcca gctccgcgcc ggtctccccg
     9961 tcgaacaccg tcaggtactc ggggccgtgc acgacgaagc cctcgaactc gcgcagctcg
    10021 ttgcgcgcgc tgcgcgaggg cgcgtactcg tcgaggaagt ggtccgcgag ggccccggcg
    10081 tccgcctcgc tcagggggta gtcgtagcgg ggttccccgc cgaaggcctc ctccagggtg
    10141 gcgggccagt tcccggccgc cacctcgggg tgctcgtgcc agccccggaa catctgcacg
    10201 acgtgctcgc ggtagtcctc ggagctcatc cggtagtcgt cgccgtgatc gaccccggcc
    10261 tcgacgtcct cctcgggcag ggtgatgtgg tccccggccg ctccgccgtc ctcgtcgaag
    10321 gtcagcgtcc gggtgcccgg ggcggtcttg agcatgatct ccgagcggcc gtcgccgtcg
    10381 aggtcgtaga ccacgaactg cgtgtagtgc gcgcccgacc ggatgttgac gcccaggtcg
    10441 atgcggtgca gcagctcgcc gtccagcgtg taggtgtcca cgtacgtcgg gccggtgtag
    10501 ccccgctggg agacgtcctt ggagttggtg gggtcccact tgaccacgaa ctcgtactgt
    10561 ccgtcgccgt ccacgtcgcc gacgctgacg tcgttggcgg tgtagccgta ctcctccccc
    10621 gccggggtgg tgccaccctc gggcctctgg agcggcaggt cgtggtggcc ctcggcccac
    10681 acggcggcct cgtcctcggg gccggtgccg ggcctccccc gggggacggg gaccacccgg
    10741 tactcggagt cggcgccgcc ctcctcgtcc aggaagttgg tgctgtcgcg cacgatcgcg
    10801 atgacgtcgc cgtcccggta cacccggaac tgcgggcccc ggagcccggc gtcggtgtgc
    10861 ccgtcgacct cgg
//
