LOCUS       NZ_JAMXJO010000001     10378 bp    DNA     linear   CON 29-AUG-2024
DEFINITION  Nocardiopsis dassonvillei strain RACA_4 assembly, whole genome
            shotgun sequence.
ACCESSION   NZ_JAMXJO010000001
VERSION     NZ_JAMXJO010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nocardiopsis dassonvillei
  ORGANISM  Nocardiopsis dassonvillei
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsaceae; Nocardiopsis.
REFERENCE   1  (bases 1 to 6726007)
  AUTHORS   Elfeky,H.H., Hanora,A. and Solyman,S.M.
  TITLE     Bioactivity of bacteria associated with Red Sea nudibranchs and
            whole genome sequence of Nocardiopsis dassonvillei RACA-4
  JOURNAL   Mar Genomics 67, 101004 (2023)
   PUBMED   36521348
REFERENCE   2  (bases 1 to 6726007)
  AUTHORS   Elfeky,H., Hanora,A.S. and Solyman,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2022) Microbiology, Suez Canal University, 4 Km
            Circular Road, Ismailia 41511, Egypt
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Assembly Name                     :: SCU_Nocdas_4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: GCA_900638215.1
            Genome Coverage                   :: 7.6x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024181525.1-RS_2024_08_29
            Annotation Date                   :: 08/29/2024 06:43:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,838
            CDSs (total)                      :: 5,775
            Genes (coding)                    :: 5,721
            CDSs (with protein)               :: 5,721
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 7 of 54
            Pseudo Genes (incomplete)         :: 51 of 54
            Pseudo Genes (internal stop)      :: 0 of 54
            Pseudo Genes (multiple problems)  :: 4 of 54
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:23:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 630022
            Orig. end                         :: 640400
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAMXJO010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10378
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="3"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10378
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10378
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5378](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5378)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(80..1477)
                     /locus_tag="NGM33_RS02685"
                     /old_locus_tag="NGM33_02685"
     CDS             complement(80..1477)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014909513.1"
                     /locus_tag="NGM33_RS02685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_02685"
                     /product="MFS transporter"
                     /protein_id="WP_130397859.1"
                     /transl_table=11
                     /translation="MNTVLRSTGSPQAIGALAVLCLAQFLIALDYSIIYVAMPSVGVDL
                     GLSSSVLQWIVSAYALLFAGLLLVGGRVCDRVGAKRVFLVSVVVFGAASALGGFAQEGV
                     ALLVSRGVQGLSAAFLQPATIGLISATFAAGPARSRALSVWGAIGASGLAVGAILGGVL
                     TEVSWRWTFLINIPLTLLAALGGLLWLAASSQRERGARVPLSASMMGTGALLALVVGLT
                     LVADTEASGLYVGGACLVAVVLGLAFIANEMKSDNVMVDRSLRRTRSLGAGVVATYLYM
                     ASVGSEFYLVTLLLQELEGFTPLQAGLGFLPLAVMVTLGNMAAGRAVERVGAGTTMTAG
                     FLISFIGLALLAAMLGSGTYATHLVPGLLLSGFGHGVIYTSMFVIGTRDVPDRHQSTAG
                     ALMTTSQYLSAAVTVALLTIILGTDPTATEFRWAFVLTAVFAVAGMLISPLLLRRPEGK
                     RAAEARR"
     gene            complement(1474..2163)
                     /locus_tag="NGM33_RS02690"
                     /old_locus_tag="NGM33_02690"
     CDS             complement(1474..2163)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014909460.1"
                     /locus_tag="NGM33_RS02690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_02690"
                     /product="HAD family hydrolase"
                     /protein_id="WP_253793228.1"
                     /sec_met_domain="HAD_2 (E-value: 3e-33, bitscore: 113.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRVRGVLLDLDGTLADSPSVILSAFHDACAPYGAVPLPADARAL
                     IGKPLDAILPRLIPGGDEAVWEEAKALFRESFARRSRTAASSLVFPGIRDLLAELSAEG
                     VGIAVVTSKITRSAHELLAATGLDGYPDVVVGHDQTPRGKPAPDPALLAAEALGLAPRE
                     CVVVGDSPDDVAMAVGAGMAVVGVGWGVSDAEALLSAGADSVATTVTELAGRLHGPVME
                     TARKVTR"
     gene            complement(2160..3116)
                     /locus_tag="NGM33_RS02695"
                     /old_locus_tag="NGM33_02695"
     CDS             complement(2160..3116)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014912831.1"
                     /locus_tag="NGM33_RS02695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_02695"
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="WP_253793231.1"
                     /transl_table=11
                     /translation="MNGTGQSGVVVSDEVRTAIEDGGPVVAMETSVVAGGSYPNNLETA
                     LAVDRCAREEGAVPARVAVIDGVLRVGVSDTELERLAKAPDDQKISTRDLAVALHGGRT
                     GGTTVSSSLIGTVAAGIRVFAVAGIGGVHRGAEVSFDVSADLTEFARSPVAVVCAGAKS
                     LLDPALTLEYLETAGVPVIGYRSDDFPGYYTRSTGQPVPCRIDDLGHLAAVMHRHWGTV
                     SDSSVLVTHPIEEEWSIDADLLNTMVEDALDEARTAGATGPALTPFVLSAITRATDGRS
                     SLVNRAVLESTTRLAAGLAVAEAATRSAEPAGPAVRS"
     gene            complement(3128..3808)
                     /locus_tag="NGM33_RS02700"
                     /old_locus_tag="NGM33_02700"
     CDS             complement(3128..3808)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908965.1"
                     /locus_tag="NGM33_RS02700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_02700"
                     /product="transglutaminase family protein"
                     /protein_id="WP_130397862.1"
                     /transl_table=11
                     /translation="MTETHTLTEPTYFLDHQSPEVREFVDRALPDRDASPREMAVRLHD
                     AVRDGVRYEVYDADLTREGVRASSVLRRGQGFCVHKSIVYAAAARSVGIPTRLVYGDVR
                     NHLASARLLDLMGDDLFTFHALVSVRIDGSWIRATPVFNTLLCRLYGIRPLEFNGRDDS
                     YYHPFDTRGRRHMEFVRWRGEYDDFPYDTVVGGIAANHPKLVGPDLVTAEGSLTDDARR
                     ERGE"
     gene            complement(3822..4985)
                     /locus_tag="NGM33_RS02705"
                     /old_locus_tag="NGM33_02705"
     CDS             complement(3822..4985)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014910981.1"
                     /locus_tag="NGM33_RS02705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_02705"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_130397863.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 6.3e-11, bitscore:
                     41.3, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 1.1e-17, bitscore:
                     62.4, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 8.1e-32, bitscore:
                     108.9, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVITTEAPPQGGDTAGARIRERVERAAPAVNRGHLERDRGAVFSR
                     EGWKELAGTGLFGSCLPEDVGGHACSLTDLMRTVEVLGYTVRDSGLNFSAATHLASTGF
                     ALSAFASPELRLRHLPSVAAGTSVGCHAITEPETGSDVLAMSATGRVDGDHVELDGLKT
                     YVTNGPVADLAVVYVRTSDTPGPLGLSAVLVPRGTPGADFGPPLDKTTLRTSPFGRLRL
                     TGCRVPRANVIGGVGTGFLILDRVMTWEILIAFVINVGEMRHRLDRVVERVGTRRQFGR
                     PLADFQGVRNTVVEMHIAVQSARQALYSAGAAVSAGHRATAETAVAKILTSEANIRTAQ
                     AAVELFGGEGVLTDTGLEIGVRDALSGPIYSGSNAVQRQKIAKALGL"
     gene            complement(5001..5378)
                     /locus_tag="NGM33_RS02710"
                     /old_locus_tag="NGM33_02710"
     CDS             complement(5001..5378)
                     /GO_function="GO:0033990 - ectoine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014911885.1"
                     /locus_tag="NGM33_RS02710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_02710"
                     /product="ectoine synthase"
                     /protein_id="WP_130397864.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.9e-29, bitscore:
                     100.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKRSREKTETVDWGNGTSDRLLVEDDNLGFTVAHTVVRAGTVSR
                     LQYANHLEACYCIAGSGLVESADGGVRIDLSPGVLYALDQHDPHLLIASEHEDLELVSV
                     FNPPLRGDERHDLDALEFSSY"
     gene            complement(5423..5683)
                     /locus_tag="NGM33_RS02715"
                     /old_locus_tag="NGM33_02715"
     CDS             complement(5423..5683)
                     /NRPS_PKS="Domain: PP-binding (30-78). E-value: 3.7e-07.
                     Score: 22.4. Matches aSDomain:
                     nrpspksdomains_NGM33_RS02715_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1147: putative acyl carrier protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017536028.1"
                     /locus_tag="NGM33_RS02715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_02715"
                     /product="phosphopantetheine-binding protein"
                     /protein_id="WP_130397865.1"
                     /sec_met_domain="PP-binding_2 (E-value: 0.029, bitscore:
                     13.0, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIDIRDRIQDRIVTAHAPDLAGEGIPGDYDLLANGVVDSLSLLE
                     LVEWIQSSFGIPVTDIEISPSDFRTIDAIADFVGSHTSQRV"
     aSDomain        complement(5450..5593)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NGM33_RS02715_PP-binding.1"
                     /evalue="3.70E-07"
                     /label="NGM33_RS02715_PP-binding.1"
                     /locus_tag="NGM33_RS02715"
                     /protein_end="78"
                     /protein_start="30"
                     /score="22.4"
                     /tool="antismash"
                     /translation="DLLANGVVDSLSLLELVEWIQSSFGIPVTDIEISPSDFRTIDAIA
                     DFV"
     gene            complement(5680..6966)
                     /locus_tag="NGM33_RS02720"
                     /old_locus_tag="NGM33_02720"
     CDS             complement(5680..6966)
                     /NRPS_PKS="Domain: AMP-binding (90-328). E-value: 1.1e-45.
                     Score: 147.9. Matches aSDomain:
                     nrpspksdomains_NGM33_RS02720_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017536029.1"
                     /locus_tag="NGM33_RS02720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_02720"
                     /product="AMP-binding protein"
                     /protein_id="WP_130397866.1"
                     /sec_met_domain="AMP-binding (E-value: 4.3e-51, bitscore:
                     172.1, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALRFADGSTTYEELVASVNAAVPGLSPDDVHVVRAGRDGRAVVE
                     ALAAQTLPRPALLVPHDAGAEYTARIGQDLRAFADGPLRSRDAHGLWTTTSGSTGRPKI
                     VAHDPGGIDRFVRWARSRFALGADTVSLSLSPINFDIATLDVWAVLAAGGQVVFAPQED
                     LTNGAALAAAARRHGVSLLQAVPVVLTLIARAGVPAPGVRTVVSTGDFYPLETLSDLES
                     AFPGAELWSVYGSTETNDSFMVDLRRATAGELGEPLDGVRYDIDGDGTGELVVSTPFQA
                     LGYVNAPNDAWRTVEGRREFRTGDLVTTDADGTLRLTGRSDRQVKVRGVRLSLDAVEAV
                     LRHQNGVADAVVTARGKGSGDRELLAAVLVEPGAAVTNLALRSAVSSALSPTAVPSRTF
                     LTTEPFPVTGTGKTDRNSTIRRLLEGQPT"
     CDS_motif       complement(5953..6015)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NGM33_RS02720_0002"
                     /evalue="6.20E-07"
                     /label="NRPS-A_a8"
                     /locus_tag="NGM33_RS02720"
                     /protein_end="338"
                     /protein_start="317"
                     /score="21.9"
                     /tool="antismash"
                     /translation="TGRSDRQVKVRGVRLSLDAVE"
     aSDomain        complement(5983..6696)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NGM33_RS02720_AMP-binding.1"
                     /evalue="1.10E-45"
                     /label="NGM33_RS02720_AMP-binding.1"
                     /locus_tag="NGM33_RS02720"
                     /protein_end="328"
                     /protein_start="90"
                     /score="147.9"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="LWTTTSGSTGRPKIVAHDPGGIDRFVRWARSRFALGADTVSLSLS
                     PINFDIATLDVWAVLAAGGQVVFAPQEDLTNGAALAAAARRHGVSLLQAVPVVLTLIAR
                     AGVPAPGVRTVVSTGDFYPLETLSDLESAFPGAELWSVYGSTETNDSFMVDLRRATAGE
                     LGEPLDGVRYDIDGDGTGELVVSTPFQALGYVNAPNDAWRTVEGRREFRTGDLVTTDAD
                     GTLRLTGRSDRQVKVR"
     CDS_motif       complement(6649..6684)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NGM33_RS02720_0001"
                     /evalue="4.20E-04"
                     /label="NRPS-A_a3"
                     /locus_tag="NGM33_RS02720"
                     /protein_end="106"
                     /protein_start="94"
                     /score="13.3"
                     /tool="antismash"
                     /translation="TSGSTGRPKIVA"
     misc_feature    complement(7546..7556)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     gene            7738..7887
                     /locus_tag="NGM33_RS02725"
                     /old_locus_tag="NGM33_02725"
     CDS             7738..7887
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NGM33_RS02725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="NGM33_02725"
                     /product="hypothetical protein"
                     /protein_id="WP_156095250.1"
                     /transl_table=11
                     /translation="MDRTTRFLWAVGVAIVIALAGGGSALAQDTADPVDGPGGASSGNG
                     AGWQ"
     gene            7967..8791
                     /locus_tag="NGM33_RS02730"
                     /old_locus_tag="NGM33_02730"
     CDS             7967..8791
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014911965.1"
                     /locus_tag="NGM33_RS02730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_02730"
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="WP_253793235.1"
                     /transl_table=11
                     /translation="MADFTVLGGLEILREDRACTPKAPKVLQVLALLLVRANRPLPMGA
                     LIRELWRDHPPKSAVTTVQTYIYQLRKVIERAQPPAHEPEVLLTRSSGYLLRVDPERLD
                     AMVFQRLLGEGRRLLEEGRPDEASKRLGEALDLWSGPCLSHVPRGPLIGPYAVRLEEQR
                     LHAQELRVRADVLLGRDRESIGELRSLVMEYPLNEWFHSQLIRALSRAGRRDEALTAYR
                     WLRLALAEELGLEPSHELQSLHQEVLGASPAPPARRRRPHARRDAPVPLAVK"
     misc_feature    8252..8254
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(8817..9794)
                     /locus_tag="NGM33_RS02735"
                     /old_locus_tag="NGM33_02735"
     CDS             complement(8817..9794)
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017536031.1"
                     /locus_tag="NGM33_RS02735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGM33_02735"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_253793238.1"
                     /transl_table=11
                     /translation="MFAEPRAGVSDLGRLLDWPVERVRSALDELARLSLVRRSWEQSEK
                     LVLVRPEFGLQSLLWQQEADLLERQQQLAATRSAITQLVSEFEESHRIRAATDIEQLHG
                     IDAIRLRIEEFAHECRHSFWTFADGGAQTRDNLEASLPLDRMLLERGVELRDVYLDSVM
                     NDPRTVRHISQLSELGARIRTVPTLPTRMILFDRTTALIPADPDDSSASALVLRGSGAV
                     TALLALYEYVWDAASPLGSSAPRGRHGLNTQEQAVVNLLAEGNTDETIARRLGVSVRTA
                     RRITADLSRRLGARSRFQAGVLATALGWVDTSSLDGAAEGEKVG"
     misc_feature    9942..9956
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.4"
                     /tool="antismash"
     misc_feature    complement(9996..10009)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.56"
                     /tool="antismash"
     misc_feature    complement(10067..10081)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.31"
                     /tool="antismash"
     misc_feature    10071..10086
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.55"
                     /tool="antismash"
     misc_feature    complement(10160..10176)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 19.14"
                     /tool="antismash"
     gene            10189..10362
                     /locus_tag="NGM33_RS02740"
                     /old_locus_tag="NGM33_02740"
     CDS             10189..10362
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NGM33_RS02740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="NGM33_02740"
                     /product="hypothetical protein"
                     /protein_id="WP_253793241.1"
                     /transl_table=11
                     /translation="MRADQAELVFFLHEDIGDRATETVLRLWKDAVRGIPSLEAWTVLE
                     TGPVHPFDPGLS"
ORIGIN
        1 gtgtcgggct cggccgaacg ccagatctcc gactcgggaa cggggtcgtt gtggatgacg
       61 agtatgccga gcctcgtcct catcggcggg cctccgctgc tcgtttcccc tccggacggc
      121 gcaggagcag cggggagatg agcatgcccg cgacggcgaa gacggcggtg aggacgaacg
      181 cccaccggaa ctccgtggcc gtcgggtcgg tgcccaggat gatcgtcagc agggccaccg
      241 tgaccgccgc cgacaggtac tgggacgtgg tcatgagcgc tccggcggtg ctctggtgtc
      301 ggtcgggcac gtcacgggtg ccgatgacga acatggaggt gtagatgacg ccgtggccga
      361 acccgctcag cagcagaccg ggcaccaggt gggtggcgta cgtgcccgat cccagcatgg
      421 cggccagcag cgccaggccg atgaagctga tcaggaagcc cgccgtcatc gtcgttcccg
      481 cgccgacgcg ctccaccgcg cggcccgcgg ccatgttccc caaggtcacc atgacggcca
      541 gcggcaggaa gcccagcccc gcctgtagcg gcgtgaagcc ctccagctcc tggaggagca
      601 gcgtgaccag gtagaactcc gatcccacac tggccatgta cagataggtg gccaccacgc
      661 cggctccgag ggaccgggta cggcgcagcg accggtccac catcacgttg tcggacttca
      721 tctcgttggc gatgaaggcc aggcccagga ccaccgccac caggcacgcg cccccgacgt
      781 acaggcccga ggcctccgtg tccgccacca gggtcaggcc gacgaccagg gccaggagcg
      841 cgccggtgcc catcatcgag gcggagaggg gaacccgggc gcccctctcc ctctgggagg
      901 acgcggccag ccacagcagc ccgcccagcg cggccagcag ggtcaggggg atgttgatga
      961 ggaacgtcca gcgccaggag acctcggtga ggaccccgcc caggatggcg ccgacggcga
     1021 ggcccgaggc gccgatcgcg ccccagaccg acagggcccg ggaccgggcc ggaccggccg
     1081 cgaaggtcgc gctgatgagg ccgatggtcg ccggttgcag gaaggcggct gacagcccct
     1141 gcacacctcg gctgaccagg agcgcgacgc cctcctgggc gaacccgccc agggcgctgg
     1201 ccgcgccgaa gacgacgacc gagacgagga agacgcgctt ggccccgaca cggtcgcaga
     1261 cacgtcctcc caccagcagg aggccggcga acaggagggc gtaggcgctg acgatccact
     1321 ggaggacgga tgacgacagg cccaggtcga cgccgacact gggcatggcg acgtagatga
     1381 tcgagtagtc cagcgcgatg aggaactgcg ccaggcagag gacggcgagc gcgccgatcg
     1441 cctggggact ccccgtggat ctgagaaccg tgttcatcga gtcactttcc tggcagtttc
     1501 catgaccgga ccgtgcaggc gcccggcgag ttcggtgacg gtggtggcga cggagtcggc
     1561 acccgccgag agtagggctt cggcgtcgga gaccccccag ccgaccccga ccaccgccat
     1621 gccggcgccg accgccatgg cgacgtcgtc gggggagtcg cccacgacca cacactcccg
     1681 gggcgccagc ccgagggcct ccgcggccaa cagagccggg tccggggcgg gcttccccct
     1741 gggggtctga tcgtgcccga ccaccacgtc cgggtagccg tccagcccgg tggccgccag
     1801 cagctcgtgg gcgctccggg tgatcttgct ggtgaccacc gcgatcccga ccccctccgc
     1861 ggacagctcc gccaacaggt cccggatccc cgggaacacc agggacgagg cggcggtccg
     1921 gctgcgacgg gcgaaggact cccggaagag cgccttggcc tcctcccaca ccgcctcgtc
     1981 tccccccggg atgagccggg gcaggatcgc gtcgagcggt ttgccgatca gcgcgcgggc
     2041 gtcggcgggc agcggcaccg cgccgtaggg ggcgcacgcg tcgtggaagg cggacaggat
     2101 caccgagggc gagtccgcca gggtgccgtc caggtccagg agcacaccgc gtacgcggct
     2161 catgaccgca ccgccggacc cgcgggttcg gcggagcggg tggcggcctc cgcgaccgcg
     2221 agcccggcgg ccagacgggt cgtggactcc aggaccgcgc ggttgaccag ggagctgcgt
     2281 ccgtcggtcg ccctggtgat ggccgacagg acgaacgggg tgagcgcggg acccgtcgct
     2341 ccggccgtgc gggcctcgtc cagcgcgtcc tccaccatcg tgttgagcag gtcggcgtcg
     2401 atgctccact cctcctcgat ggggtgggtg accagtacgg aggagtcgga gaccgtcccc
     2461 cagtgacggt gcatgaccgc ggccaggtgc cccaggtcgt cgatccggca ggggacgggc
     2521 tgtcccgtgc tgcgcgtgta gtagccgggg aagtcgtcgg accggtaccc gatgacggga
     2581 acacccgcgg tttccaggta ctccagggtc agggcggggt cgagcaggga cttcgccccc
     2641 gcgcagacga ccgccacggg cgagcgcgcg aactcggtga ggtcggcgga gacgtcgaag
     2701 gagacctcgg cgccccggtg tacgcctccg atccccgcga ccgcgaacac cctgatcccc
     2761 gcggcgaccg tgccgatgag gctggaggag accgtggtgc cgccggtgcg gccgccgtgc
     2821 agggccacgg ccaggtcgcg ggtactgatc ttctggtcgt ccggggcctt ggccaggcgc
     2881 tccagctccg tgtccgagac cccgacgcgc agaacgccgt cgatcaccgc gacacgggcg
     2941 gggacggccc cctcctcacg cgcgcaccgg tccaccgcca gcgcggtctc caggttgttc
     3001 gggtaggagc cgccggcgac cacggaggtc tccatggcga cgaccggtcc gccgtcctcg
     3061 atcgcggtac gcacctcgtc ggagacgacg acgcccgact gtccggttcc gttcacgtcc
     3121 actcctctca ctctccgcgc tcacggcggg cgtcgtcggt cagcgaccct tcggcggtca
     3181 ccaggtccgg tccgacgagc ttgggatggt tggcggcgat accgccgacc acggtgtcat
     3241 aggggaagtc gtcgtactcg ccgcgccatc ggacgaactc catgtggcgt cggccccggg
     3301 tgtcgaaggg gtggtagtag ctgtcgtcgc ggccgttgaa ctccagcggc cggatgccgt
     3361 agaggcggca cagcagggtg ttgaagacgg gggtcgcacg gatccagctg ccgtcgatgc
     3421 gcaccgacac cagggcgtgg aaggtgaaga ggtcgtcgcc catgaggtcc aggaggcggg
     3481 cggaggccag gtggttgcgc acgtccccgt agaccagacg cgtggggatg cccaccgagc
     3541 gggcggcggc cgcgtagacg atggacttgt ggacgcagaa cccctgcccg cgtctgagca
     3601 cgctggacgc acggacgccc tcccgggtga ggtcggcgtc gtagacctcg tagcggacac
     3661 cgtcgcgtac ggcgtcgtgc aggcgtacgg ccatctcccg tggggaggcg tcacggtccg
     3721 gcagcgcccg gtcgacgaac tcccgcacct cggggctctg gtggtcgagg aagtaggtcg
     3781 gttcggtcag tgtgtgggtc tcggtcacgg cggtcctcct gtcacaggcc cagggccttg
     3841 gcgatcttct gccgttgcac ggcgttggag ccggagtaga tggggccgct gagggcgtcg
     3901 cggaccccga tctccaatcc ggtgtcggtg agcacgccct cgccgccgaa cagttcgacc
     3961 gccgcctggg cggtacggat gttggcctcg ctcgtgagga tcttcgccac cgccgtctcc
     4021 gcggtcgccc tgtgtccggc ggagaccgcc gcgcccgcgg agtacagggc ctggcgggcg
     4081 ctctggacgg ctatgtgcat ctccacgacg gtgttgcgca ccccctggaa gtcggccagc
     4141 ggccgaccga actggcgccg ggtgccgacc cgttccacga cccggtccag acggtggcgc
     4201 atctcgccca cgttgatgac gaacgcgatg aggatctccc aggtcatcac gcggtccagg
     4261 atcaggaagc cggtacccac gccaccgatg acgttggccc tggggacacg gcaccccgtc
     4321 aggcgcagtc gtccgaaggg ggacgtccgc agggtcgtct tgtccagcgg cggaccgaag
     4381 tccgcgccgg gcgtccccct cgggacgagt acggcgctca ggccgagcgg accgggggtg
     4441 tcggaggtgc ggacgtagac gaccgccagg tcggcgaccg ggccgttggt gacgtaggtc
     4501 ttcagaccgt ccagttcgac gtggtcgccg tcgacgcgac cggtggcgga catggccagc
     4561 acgtccgacc cggtctcggg ctcggtgatg gcgtggcagc cgacggaggt cccggcggcg
     4621 accgaaggca ggtggcgaag gcgcagttcg ggactggcga aggcgctgag cgcgaacccg
     4681 gtactggcga ggtgggtggc cgcgctgaag ttcagtccgg agtcgcggac ggtgtaaccg
     4741 aggacctcga ccgtgcgcat gaggtcggtg agggagcagg cgtgtccgcc gacgtcctcg
     4801 ggcaggcacg aaccgaacag gcccgtgccc gccagctcct tccacccctc ccgggagaag
     4861 accgcgccgc ggtcccgttc gaggtggccc cggttgacgg cgggcgcggc gcgttcgacg
     4921 cgttcgcgta tgcgggcgcc cgcggtgtcc ccgccctgcg gcggtgcctc tgtggtgatc
     4981 accatgctgt ccacatccgg tcagtaggag gagaactcga gtgcgtcgag gtcgtgccgc
     5041 tcatcgccgc ggagcggggg gttgaacacg ctgacgagtt ccaggtcctc gtgttcgctg
     5101 gcgatcagca ggtgcgggtc gtgctggtcc agtgcgtaca ggacccccgg ggagaggtcg
     5161 atgcgcacac cgccgtccgc gctctcgacc agcccgctgc ccgcgatgca gtagcaggcc
     5221 tccaggtggt tcgcgtactg gagtctggag acggtccccg cccgcaccac ggtgtgcgcg
     5281 acggtgaacc ccaggttgtc gtcctccacc agcagccggt cgctggttcc gttgccccag
     5341 tcgacggtct cggtcttctc acgggagcgc ttgagcatgt cgtttcctct ttcctggtct
     5401 gctggaaggc ccgcggtgcg tgtcagaccc gctggctggt gtgggacccg acgaagtccg
     5461 cgatcgcgtc gatggtgcgg aagtccgagg gggagatctc gatgtcggtg acggggatgc
     5521 cgaaggagga ctggatccac tccacgagtt cgaggaggga gaggctgtcg acgacaccgt
     5581 tggcgaggag gtcgtagtcg ccggggatgc cctctcccgc caggtccggg gcgtgggcgg
     5641 tcacgatgcg gtcctggatc cggtcccgga tgtcgatggt catgtcggct gcccttcgag
     5701 gagtcttcgg atggtgctgt tccggtcggt ctttcccgtg ccggtgacgg ggaagggttc
     5761 cgtggtgagg aacgtccggc tgggtaccgc tgtcgggctc agggccgagg agacggccga
     5821 gcgcagggcg aggttggtca cggcggcccc cggctcgacg agcacggccg cgaggagttc
     5881 cctgtccccg gagcccttgc cccgtgccgt gaccacggcg tcggccacac cgttctggtg
     5941 gcgcagcacg gcttcgaccg cgtccaggga caggcgcaca ccgcgcacct tgacctggcg
     6001 gtcgctccgt ccggtcagac gcagtgtgcc gtcggcgtcc gtggtgacca ggtcgcccgt
     6061 gcggaactcg cgccgcccct cgacggtgcg ccaggcgtcg ttcggggcgt tgacgtaccc
     6121 cagcgcctgg aagggcgtgg acacgaccag ttcaccggtg ccgtccccgt cgatgtcgta
     6181 gcggactccg tcgaggggct cgcccagctc cccggccgtc gcccggcgca ggtccaccat
     6241 gaagctgtcg ttggtctcgg tgctgccgta gacgctccac agttcggcgc cggggaaggc
     6301 cgactccagg tcggacaggg tctccagggg atagaagtca ccggtggaga ccaccgtgcg
     6361 gacccccggc gcggggacgc cggcacgggc gatgagggtg aggacgaccg gcacggcctg
     6421 gaggaggctg acaccgtgcc ttcgggccgc cgcggccagg gccgcgccgt tggtgaggtc
     6481 ctcctggggg gcgaagacga cctgcccgcc cgcggccagg accgcccaca cgtccagggt
     6541 ggcgatgtcg aagttgatcg gcgagaggct gagggagacc gtgtcggccc ccagggcgaa
     6601 gcgggagcgc gcccagcgga cgaagcggtc gatgccgccg gggtcgtggg cgacgatctt
     6661 gggcctgccc gtcgagccgg aggtggtggt ccacagtccg tgggcgtcgc gcgaccgcag
     6721 ggggccgtcg gcgaaggcgc gcaggtcctg gccgatccgg gcggtgtact cggcacccgc
     6781 gtcgtgcggg accaggagcg cgggacgcgg gagggtctgg gcggccaggg cctcgaccac
     6841 ggcccgtccg tcacggccgg cccgcacgac gtggacgtcg tcgggggaga gcccgggcac
     6901 ggccgcgttc accgacgcga ccagttcctc gtaggtcgtg ctgccatcgg cgaacctcaa
     6961 cgccacggat tcgggccgat ggcgggcgtg gtcttggatt gatgcccata cggcaggagt
     7021 agccatggtt ttcggctcct caattgcggg tccaggggat gtgcgtccac aaatgcacag
     7081 gacgcgtgga acggcctgtt caggccggtc cggggtgggg gttctgcctg attcgggtgg
     7141 ctgacacgaa gatttctcgg tcgtccagag cagaacatag gcaacgccgc cgaggcttgg
     7201 caatgctctt cagagaaaat ccccggcgcc cggaccagcg cacggataac cgcggacccc
     7261 ggacaggggg tttctcattt cccgggaccg ttgttcgacc gtgcgtggac gggggctcga
     7321 gcggtcctcg aacgcggttc cggctcccgc cccgcacccg gaggcagtcg cggcgctgct
     7381 cctggcgcgg cgggccgggg cgccggggcc cccgccgcga ccgctccgcg cggcgcggcc
     7441 cgggacgggg cggtcccgcg gccgggagcg gcgccttcca gcgccgggcg cgtagccccg
     7501 gaagcgggcg ggggccacgg gcgcaagcgc ggctctggca gctttgtgcc attgcaggag
     7561 catgccagta gatcgtgctg acattccccg gccgagtgcc gatcatggaa ctcgggcagg
     7621 cgggacggga acgctgattt cccggtccgc ctggtcgcgg agcgaattcc ataagtcata
     7681 cagatcactg tcgttgctgt ggaaacaccc ggcacagaga aaagggaagt gaccttcatg
     7741 gacaggacca cgcgtttcct ctgggcggtc ggcgtcgcga tcgtcatcgc actcgccggc
     7801 ggcggttccg ccctcgccca ggacaccgcg gatcccgtgg acggccccgg gggcgcctcc
     7861 agcgggaacg gcgccggctg gcagtgaacc cgggcggacg agggggcgcc atcgcggggg
     7921 gaccccggct cccgttgtcg gaacatcggt cgtagggaga gatgacatgg cggacttcac
     7981 ggttctcggc gggctggaga tcctgaggga ggaccgggcg tgcacgccca aggcgccgaa
     8041 ggtgctgcag gtcctggcgc tgctgctggt gcgggcgaac cgtccgctgc ccatgggcgc
     8101 cctgatcagg gaactgtggc gggaccaccc gcccaagagc gcggtgacga ccgtgcagac
     8161 gtacatctac cagctccgca aggtcatcga gcgggcgcag ccccccgctc acgaacccga
     8221 ggtcctcctc acccgctcct ccgggtacct gttacgagtc gaccccgagc ggttggacgc
     8281 gatggtgttc cagcggctgc tcggcgaggg gcgccgcctc ctggaggagg gccgacccga
     8341 cgaggcctcc aagcgcctgg gcgaggcact ggacctgtgg tccgggccgt gcctgtccca
     8401 cgtcccccgc ggaccgctga tcggccccta cgcggtccgg ttggaggagc agcggctgca
     8461 cgcgcaggaa ctgcgcgtgc gggccgacgt cctgctgggc cgtgaccggg agtcgatcgg
     8521 tgaactgcgg tccctggtga tggagtatcc gctgaacgag tggttccaca gccagctcat
     8581 ccgcgccttg agccgggccg gacgccgcga cgaggcgctg acggcctacc gctggctgcg
     8641 gctggccctc gccgaggaac tcggcctgga gccctcccac gaactgcaga gcctgcacca
     8701 ggaggtgctc ggcgcgagtc cggcgccccc ggcccgtcgg cggcggccgc acgcccggcg
     8761 ggacgcgccc gttcccctcg cggtgaagtg aagcccgccg ccgggcgggc cgctgctcag
     8821 cccaccttct ccccttccgc ggctccgtcg agggaggagg tgtcgaccca gcccagcgcg
     8881 gtcgccagga caccggcctg gaaacggctc cgggcgccca gccgtcggct gaggtccgcg
     8941 gtgatccggc gtgccgtgcg caccgacacc ccgagcctgc gggcgatggt ctcgtcggtg
     9001 ttgccctccg ccagcaggtt caccaccgcc tgctcctggg tgttcagacc gtgacggccg
     9061 cgcggggcgc tggagcccag ggggctggcc gcgtcccata cgtactcgta cagggccagc
     9121 agggccgtga cggcgcccga accgcgcagg accagggcac tggcggagga gtcgtcggga
     9181 tcggcgggga tcagcgcggt ggtgcggtcg aagaggatca tcctggtggg cagggtgggt
     9241 accgtgcgta tccgggcgcc gagctcggac agctgggaga tgtgccggac cgtcctggga
     9301 tcgttcatca cgctgtcgag gtagacgtcc cggagttcga ccccccgttc gagcagcatc
     9361 cggtccaggg gcaggctcgc ctccaggttg tcccgcgtct gcgcgccccc gtccgcgaac
     9421 gtccagaacg agtggcggca ttcgtgcgcg aactcctcga tccgcagccg gatcgcgtcg
     9481 atcccgtgga gctgctcgat gtccgtggcc gcgcggatgc ggtggctctc ctcgaactcg
     9541 gagaccagct gggtgatggc cgagcgcgtg gcggccagct gctgctggcg ttccagcagg
     9601 tcggcctcct gctgccacag cagcgattgc aggccgaact cgggccgtac caggaccagt
     9661 ttctcggact gctcccacga gcgccggacc agggacagcc gggccagttc gtcgagcgcc
     9721 gaccgcacgc gttccaccgg ccagtccagg aggcgcccga ggtcgctcac tccggcccgg
     9781 ggttcggcga acatggcgcg atagacactc accgcgtctt gaccgattcc gatcgacctg
     9841 aacacgaccc tcctccagcc gttacccgtc tgccgggctc ctggaccaca cggcacgacg
     9901 agcacaccga cgtatcggag cgaagacgcg gtcctcacgg gacttccgtg aaaattatgg
     9961 agcccgtggg gcgtgcccgc aagagtactt cagggtcgtt tcgaaaaagg gtcgaaccct
    10021 tgcgcgttcc gggtgtgcta acggtgcccc aatgaacagg cggcgaataa catgagaatc
    10081 atgtgcctgc ggttggctgt ccgggatccg tttccactcc gggggatcca ggaggcggtg
    10141 tacgcctgct ccgggcccga tgacatgttg gaacacctct atgtcgaaat gcgtgccgac
    10201 caggcggaac tcgtcttttt cctgcacgag gacatcgggg accgcgcgac ggagaccgtc
    10261 ctgcggctgt ggaaggacgc cgtgcgcggt atcccctcgc tggaggcgtg gaccgtgctg
    10321 gagaccggcc ctgtccaccc cttcgacccc ggtctgtcct gacccgttcc cgccggga
//
