LOCUS       NZ_CP050861            12181 bp    DNA     circular CON 16-JUL-2024
DEFINITION  Tenacibaculum mesophilum strain bac2 chromosome, complete genome.
ACCESSION   NZ_CP050861
VERSION     NZ_CP050861.1
KEYWORDS    RefSeq.
SOURCE      Tenacibaculum mesophilum
  ORGANISM  Tenacibaculum mesophilum
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Tenacibaculum.
REFERENCE   1  (bases 1 to 3360630)
  AUTHORS   Li,M.
  TITLE     Tenacibaculum mesophilum bac2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3360630)
  AUTHORS   Li,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2020) College of Ocean and Earth Science of Xiamen
            University, Xiamen University, Xiangan South Rd, Xiamen, Fujian
            361102, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. 0.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 500.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024181065.1-RS_2024_07_16
            Annotation Date                   :: 07/16/2024 03:22:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,062
            CDSs (total)                      :: 2,988
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 74
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:51:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2063147
            Orig. end                         :: 2075328
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050861.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12181
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12181
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12181
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7181](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7181
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(105..2315)
                     /locus_tag="HER15_RS09305"
                     /old_locus_tag="HER15_09315"
     CDS             complement(105..2315)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lant_dehydr_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155: Lantibiotic dehydratase domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF016615.4"
                     /locus_tag="HER15_RS09305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HER15_09315"
                     /product="lantibiotic dehydratase family protein"
                     /protein_id="WP_253679206.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 3.2e-157,
                     bitscore: 521.9, seeds: 73, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQKKKIPYQIFDSYCLRTPLFSLSDYKSLIEREGVLETDVRNLL
                     QNKIFREALFLASPELVVQIQKWENQEILDKKKKENLELTILKYYTRITTRCTPFGLFA
                     SCSQGSFSDETSIQLAAIEDYKRITRFDTTFLAELSLELIKKDEIRKQLLFFPNSSLYK
                     VGEHYRYVEYVIQNKKRTYSIEGIKFFTYLKDVLEAAKEGNTIAALAKVLVSDDIELIE
                     AKGFIEELIEHQILVSELEVTVTGSDYFSNLIKKVSEISENEELKSKLMELSNHLNKID
                     DSFGNSASRYKKIEELSKQVTPKKGVKYLFQTDTFSKTTQNKLNRKHKSNLYKALRIFN
                     KMTLRSTSGRLEDFKRAYTKRYESEWLPLSLVLDVETGIGYGKKEENNTPFLDKIVNTP
                     KKKRYKHIVWRDVDSILQEKLFEVLTEGKTTIKLTEQDFESIEERWDDLPDTFSSIIEV
                     FKSSGEEKLFIKGTGGSSAINLLGRFGHGSEGLSQHINNIAETEKNIEKDKVIAEIVHL
                     PEARTGNILQRPNFRDYEIPYLGKSNVAKEREIPLSDILVTVKNDEIKLFSKKLNKEVI
                     PRLGNAHNYSNSTLPIYHFLCELQTQNKRASIGFSWNATFLNLPFLPRVVYKDIIFSKT
                     RWNLETDKLGEILKSKDFFKQMVNWKKELQLPNYVELVEGDNRLLIYLENKTAVQMLYN
                     AVKRKKNFVLEEFLFVDDELVKREGTSFCNQFVVSFYNNKTN"
     gene            2602..3765
                     /locus_tag="HER15_RS09310"
                     /old_locus_tag="HER15_09320"
     CDS             2602..3765
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029: RND family
                     efflux transporter MFP subunit"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /locus_tag="HER15_RS09310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HER15_09320"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_253679207.1"
                     /transl_table=11
                     /translation="MSKQIFPKEIIENSTEVHQFKHTNKSKIIYSVFLVALIITIASLP
                     FIKITIYNTSQGLIRPDKERISLQNSTNGKVIFHQLKNNQEIHEGDTLLLVNNLAITQK
                     IENIESQAHEIKTFIEDLELLSKNKKRGIQLQSLKYQQEYNFYQQKLNELRTRFKKLKD
                     DYKRNQQLFKKGVIAKVELNNSQLEYDLALNAIYQYKKQQASSWQSQLVDYQNQLKELE
                     NNHTQLQESTNLSIIKAPVSGTLLNVKGIEKGSFIPSGTQLAEISPKSDLIVECFISPS
                     DIGLLKKENKVNFQVSAFNYNQWGLATGNILEVGNDIQMVNDTPMFRVACSLNQRFLTL
                     KNGFKGKLKKGMLVNARFELTQRSLFDLLYDKMDDWLNPATQIAENK"
     gene            3879..4187
                     /locus_tag="HER15_RS09315"
                     /old_locus_tag="HER15_09325"
     CDS             3879..4187
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669623.1"
                     /locus_tag="HER15_RS09315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HER15_09325"
                     /product="DNA-binding protein"
                     /protein_id="WP_167712557.1"
                     /transl_table=11
                     /translation="MKNLKTLERLQQLHNLITNENTGTPKELANLLQISQRSVHLLVEQ
                     LKDYNANICYSRSRKTYYYCDDFDLQVSISVNVLTNNEVTQIFGGSYFLQNNLHIAS"
     misc_feature    complement(4348..4362)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 24.8"
                     /tool="antismash"
     gene            4354..4524
                     /locus_tag="HER15_RS09320"
                     /old_locus_tag="HER15_09330"
     CDS             4354..4524
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HER15_RS09320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HER15_09330"
                     /product="hypothetical protein"
                     /protein_id="WP_253679208.1"
                     /transl_table=11
                     /translation="MKNLENFGVQELNAGEVKKVQGGGWLAYALGFLTGAMIKAGEGLE
                     RSGFNSAGANK"
     misc_feature    4383..4394
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.28"
                     /tool="antismash"
     gene            4565..4714
                     /locus_tag="HER15_RS09325"
                     /old_locus_tag="HER15_09335"
     CDS             4565..4714
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HER15_RS09325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HER15_09335"
                     /product="hypothetical protein"
                     /protein_id="WP_253679209.1"
                     /transl_table=11
                     /translation="MNVIELNSNEFKQVNGGNWYRWGITLLGAMINDAQNNPDDFEAGM
                     AFWE"
     misc_feature    complement(4884..4897)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.39"
                     /tool="antismash"
     gene            5001..7181
                     /locus_tag="HER15_RS09330"
                     /old_locus_tag="HER15_09340"
     CDS             5001..7181
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073761.1"
                     /locus_tag="HER15_RS09330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HER15_09340"
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="WP_173360487.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2.9e-37, bitscore:
                     124.9, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 7.3e-163, bitscore:
                     541.1, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKITIKQHDITDCGAACLASISAHYKLQLPIARIRQYAGTDKKG
                     TNVLGLIEAAEKLGFEAKGVRGELDSLFKIPKPAIAHVIVREQLQHYVVIYEVTKEYIK
                     IMDPGDGKIHKKTHEEFLKEWTGILVLLLPKEEFSTGNEKVSVLKRFWFLLKPHKFVLL
                     QALIGAVIYTLLGFSTSIYIQKITDYVLVGGNTKLLNLLSIIMLSLLALQIIIGVYKDV
                     FLIKTGQQIDARLILGYYKHLLKLPQQFFDTMRVGEIISRINDAVKIRTFINGVSLSLT
                     VNILILFFSFGLMFMYHWKLALIMLLVIPFYLTVYFIINKLNKKTERKIMERSADLESQ
                     LVESLNSVGTIKRFGLESFANIKTETRFISLLHIGYKSALNSVFSGTSTTFIAQLFTIV
                     LLWSGSYFVIERQITAGELMSFYAIIGYFMNPVASLIGANKQIQNALIAADRLFEIMDL
                     EREENTEKVVLTKEKIGDIEFKNVAFRYGTRVQVFTDFNLKIKKGNITAIVGESGSGKS
                     TLISLLQNLYPIQKGHIALGDLDLKYIQHESLRELVSVVPQKIDLFAGNVIDNIAVGDF
                     APNMELIIEICKSIGILEFIESLPNGFDTYLGENGATLSGGQKQRIAIARALYKQPEIL
                     VLDEATSSLDSTSENYIQKAVERLRQENKTIIIIAHRLSTVINADEIVVLNKGNVLEQG
                     NHKELYVAEGKYYNLWQQQMPQIELNKKAHQI"
     tRNA            complement(7193..7263)
                     /anticodon=(pos:complement(2070376..2070378),aa:Cys,
                     seq:gca)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="HER15_RS09335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="HER15_09345"
                     /product="tRNA-Cys"
     gene            complement(7193..7263)
                     /locus_tag="HER15_RS09335"
                     /old_locus_tag="HER15_09345"
     gene            complement(7319..7681)
                     /gene="folB"
                     /locus_tag="HER15_RS09340"
                     /old_locus_tag="HER15_09350"
     CDS             complement(7319..7681)
                     /EC_number="4.1.2.25"
                     /GO_function="GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /gene="folB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015362036.1"
                     /locus_tag="HER15_RS09340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HER15_09350"
                     /product="dihydroneopterin aldolase"
                     /protein_id="WP_159610666.1"
                     /transl_table=11
                     /translation="MGIIRVNDIRVFTNHGCLDEEARIGSEYRVDIEVKADLQTSAKTD
                     NLVDTVDYVHLNKIVKEEMAIRSKLLEHVAQRILDRIFKEIQLVDEAEVSVAKINPPIG
                     GNVAEVAIVLSEVRKK"
     gene            complement(7778..10603)
                     /locus_tag="HER15_RS09345"
                     /old_locus_tag="HER15_09355"
     CDS             complement(7778..10603)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080086.1"
                     /locus_tag="HER15_RS09345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HER15_09355"
                     /product="glycosyl hydrolase"
                     /protein_id="WP_167712562.1"
                     /transl_table=11
                     /translation="MRTLAKALIALFPFTVAAQQPTSAEKIQQALQQKEALTLTSTVKN
                     IPFKNIGPTVMSGRVVDIDVNPANTTEFYVGYASGGVWHTDNNGTTFTPILDNSPTQNV
                     GDIAVDWKNGTIWVGTGENNSSRSSYAGIGILKSTDKGKTWTNVGLLDSHHIGRILINP
                     KNPDEVIVGSIGHLYSSNNERGIFKTTDGGKTWIKTLFIDENTGIIDIQPVPNNFNILY
                     AAAWERERKAWNFDGDGKNSAIYKSTDAGSTWTKISDNNGFPNGDGVGRIGLAVYDQNT
                     VYAFHDSQFRRKKDPAKKEKGAGALTKEELASISADELLNMKDKKLNSYIKMNGLQEKY
                     RAENIKQIIRSSPGEMKPSDLIGYLKDANAALFDTPVIGAEVFKTIDGGKTWKKTHEGY
                     LDDLYYSYGYYFGEIRVDPQDKNGIYVLGVPILKSKDGGKTFTSISKENVHADHQALWV
                     NPKKQGHLIDGNDGGLNISYDDGESWIKLNQPAVGQFYTVYADNQEVYKVYGGLQDNGV
                     WVGNHNAKIDKSWHQTGQNPYESLMGGDGMQVAVDDRNPNIVYTGYQFGNYYRIDRATG
                     KQTYIQPKPKKDKPAYRFNWQTPIQLSKHNQDILYLGGNKLHRSLNKGDDWEEISGDLT
                     KGEKEGNVAYGTLTTISESPFQFGLIYVGTDDGLVQVTKNGGGNWEKISNSLPQNLWVS
                     RVIASKYKKERVYVTLNGYRFDDFTPYVYMSDDYGKTWKNISSNIPASAVNVIKEDPKK
                     ENVLYLGTDNGLYVSFNQGSTWEAFSNGLPNVAVHDLVIQPKAKHLLVGTHGRSLYKAN
                     ITSLQEFDNKEAIFSMKDIKKRKSWGSSWSKWLEPNTPKITIPFYVNADKEVVLEIYSG
                     KVLVNTIKAKATKGFNEVVYDVSFSEKGRKKYLKKNKEAKVKKAKNNKYYLPKGKYKVT
                     VGELSQTFEVL"
     gene            10746..11633
                     /locus_tag="HER15_RS09350"
                     /old_locus_tag="HER15_09360"
     CDS             10746..11633
                     /GO_function="GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095356.1"
                     /locus_tag="HER15_RS09350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HER15_09360"
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="WP_073182409.1"
                     /sec_met_domain="RimK (E-value: 2.5e-60, bitscore: 200.8,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 1.1e-10, bitscore:
                     39.2, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIFILSVSDQIYSTQRIYKEASRRGHKVEIINHLKCFIKLGDGK
                     PAILYNGKNIIDIPDVIIPRIGVSAMKHGTTVVKEFEMNGVYSTASYLGIVDAQNKART
                     LQIMSRNGLPIPQTVFSVDAENIGEQIDLLGGSPVIIKLQEGTHGSGVILAESKKSAMS
                     IIDTMHGTNTSILLQEFIQESNSEDIRAFIVNGKVVSSMKRKGLEGDFRSNIHKGGSGH
                     KVTLTPEEEEIAIKAAQHLELPIAGVDMIRSKRGPLLIEVNSTPGLQGIESYTKDNIAK
                     VIIKFLEENVRTKF"
     misc_feature    complement(11671..11689)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.12"
                     /tool="antismash"
ORIGIN
        1 ttgtaacaac acattctccg ctacttcttt tccacaatat actttgtagt acacccactt
       61 ttctcctgga aaaaaatttc tttttattac aacttccatt ttcatcaatt agttttattg
      121 ttataaaaag atactacaaa ttgattgcaa aaagacgtcc cttctctctt tacaagctca
      181 tcatcaacaa ataagaactc ttctaacaca aagttctttt ttcttttaac tgcattgtat
      241 aacatttgaa ccgctgtctt attttctaaa taaataagta gtctgttgtc tccttcaact
      301 aactcaacat aattgggtaa ttgcagttcc tttttccaat tgaccatttg tttaaaaaag
      361 tctttgcttt ttaatatttc tcctaattta tcggtttcaa ggttccatct agttttagaa
      421 aaaataatat ctttatatac tactcttggt aaaaaaggta ggttcaaaaa agttgcattc
      481 caactaaatc ctatactagc tcttttattt tgagtttgta attcgcacaa aaaatgataa
      541 atcggcaatg tactattgct ataattatga gcattcccta atcttggaat tacttcttta
      601 tttaattttt tagaaaaaag tttaatctca tcgtttttaa cagtaactaa aatatcactt
      661 agtggaattt ctctttcttt agctacattg gattttccta aataaggaat ttcatagtct
      721 ctaaaattag gacgctgtaa gatatttcct gttctagcct ctggcaaatg gactatttca
      781 gctatgactt tatccttttc aatatttttt tcggtttcag ctatattatt tatgtgttgt
      841 gacaagcctt cgctaccatg accaaacctg cctaataaat ttatagcact gctacctcca
      901 gttcctttta taaacagttt ttcttcacct gaactcttaa acacttctat aattgacgaa
      961 aacgtgtcgg gcaaatcatc ccatctttct tctatacttt caaaatcttg ctccgttaat
     1021 ttaattgtag tttttccttc agtcagtacc tcaaataact tctcttgtaa aatactatct
     1081 acatctctcc aaactatatg tttatatctt ttctttttgg gagtgtttac aattttatct
     1141 aaaaaaggag tgttgttttc ttcttttttt ccataaccaa tacctgtttc aacatctaac
     1201 actaaactta acggcaacca ttcactttcg taacgttttg tataagctct tttaaaatct
     1261 tctaaccttc cacttgtaga acgcaatgtc attttattaa aaatccttaa cgctttgtat
     1321 agattgcttt tatgttttct gtttagttta ttttgagttg tttttgaaaa agtatccgtt
     1381 tggaataaat acttgacacc cttttttggg gtaacctgtt tgcttagttc ctcaattttt
     1441 ttatatctag atgctgagtt tccaaaagaa tcatctatct tatttaaatg atttgataat
     1501 tccataagtt tgcttttaag ttcttcattc tcagaaatct cagaaacttt ttttattaaa
     1561 ttagaaaagt aatcgcttcc tgtaacagta acttcaagtt cagacaccaa aatctgatgc
     1621 tcaattaact cttctataaa cccttttgct tctattaatt caatatcatc gctaacaagc
     1681 acttttgcta acgctgcaat tgtgtttcct tcttttgcag cttcaagcac atctttcaaa
     1741 taggtaaaaa acttaattcc ttcgattgaa tacgttcttt ttttgttttg tattacatac
     1801 tcaacatatc tgtaatgttc tcctacttta tataaacttg aattcggaaa aaacagtaat
     1861 tgttttctaa tttcatcttt ctttatcaac tctaaagata actctgctaa aaaagtggta
     1921 tcaaaacgag ttattctttt atagtcctct attgctgcta attgaattga tgtttcatca
     1981 ctaaaactcc cttgagaaca agaagcaaat aaaccaaaag gagtacaacg agtagttatt
     2041 cttgtataat attttaagat tgttagttct aagttttctt ttttcttctt gtcaagaatt
     2101 tcttgatttt cccatttttg aatttgcact accaattcag gagaggctaa aaataatgcc
     2161 tccctaaaaa ttttgttttg caacaaattt ctaacgtccg tttctaaaac cccttctctt
     2221 tctattaaac ttttataatc agaaagagaa aataacggag ttcttaaaca ataagaatca
     2281 aaaatttgat atggtatttt cttcttttga gccaattttt agtgtcttta tccttgcaaa
     2341 tgtagttatt attaagacaa gctcataaaa cagttaaacc cttaaaaaac agatacaaac
     2401 taacaaaatt ataactcgaa atttataaat aacgtccatt tttttataaa tctcgctata
     2461 tatggatttt ttactgcatt tattttgcgt tggtttgtag tgtattcgta gacagggaaa
     2521 gttatacgtg aaaaagaaaa accacccctt tatttttact ttttgttctc ataaataaag
     2581 gggttttatt ttaaaaaagt tatgagcaaa caaatatttc ctaaagaaat tattgaaaac
     2641 agtaccgaag tgcatcagtt taagcacacc aacaaaagta aaattattta tagtgtcttt
     2701 ttagtagctt taataattac tatagcttct ttacctttta ttaaaataac tatttacaat
     2761 acttcacaag ggttgatacg ccccgataaa gaacgaattt ctttacaaaa cagcactaat
     2821 ggtaaagtta ttttccatca acttaaaaat aatcaagaaa tacatgaagg agacacctta
     2881 ttattggtaa acaatttagc tattactcaa aaaattgaaa acattgagag tcaagcgcat
     2941 gaaattaaaa ctttcattga agatttagaa ctcttatcta aaaataaaaa acgtggtatt
     3001 caattacaat cgttaaaata tcaacaagaa tataattttt accaacaaaa attaaatgag
     3061 ttacgtactc gctttaaaaa actgaaagat gattataaac gaaatcaaca attatttaaa
     3121 aaaggagtta ttgctaaagt tgaattaaac aatagtcaat tagaatatga tttggcgctg
     3181 aatgctattt atcaatataa aaaacaacaa gctagcagct ggcaatcaca attggtagac
     3241 tatcaaaatc aattaaaaga gttagaaaac aatcatacgc aactacaaga gagtactaat
     3301 ttatcaatta taaaagcccc tgtttcgggt actttattaa atgtgaaagg aattgaaaaa
     3361 ggaagtttta ttccttctgg tacacaactc gctgaaatat caccaaaatc agatcttatt
     3421 gttgagtgtt ttataagccc aagtgatatt ggattgctaa aaaaagaaaa caaggtaaat
     3481 tttcaagtaa gtgcttttaa ttataaccag tgggggttgg caacaggaaa catactagaa
     3541 gtaggaaacg acatacaaat ggtaaacgat acgcccatgt ttagggtagc atgttcttta
     3601 aaccaacgtt ttttaacatt aaaaaatggc tttaaaggaa agctaaaaaa agggatgtta
     3661 gttaatgctc gttttgaact cactcaacgt tctttatttg acttactcta cgacaaaatg
     3721 gatgattggt taaatcctgc aactcaaatt gcagaaaata aataatctgt ctttgttaga
     3781 aataaagaag tcttcttact taaaaaataa aattacacgt cattgcgagg aacgaagcaa
     3841 tcctttcttt tagtaacgaa aaactataaa aaaagaaaat gaaaaattta aaaacactag
     3901 aacgcttaca gcaactacat aatttaatta caaacgagaa tacaggaaca cctaaagaat
     3961 tggctaactt attacaaatt agccaacgtt cggttcattt attagtagaa caattaaaag
     4021 attacaatgc gaatatttgt tatagtagaa gcagaaaaac ctactactat tgtgatgatt
     4081 ttgatttaca agtatctatc tcagtaaatg tattaacgaa caatgaggta acccaaatat
     4141 ttgggggtag ttacttttta caaaataatt tacacattgc aagttagttg cagcgaacga
     4201 gactatactt gtaacatcaa aacaaacaaa taggtgcaca tatttaggtt ggttttgata
     4261 tggattgtga ttttttatgt tatccctatt taggtgattg agcgtaatcg aaatcacagt
     4321 caaaaacaga tattaattaa aaaattaaat tttatgaaaa atttagaaaa ttttggggtt
     4381 caggaattga acgctggaga agttaaaaaa gttcaaggtg gcggttggtt agcttatgct
     4441 ttaggttttt taactggtgc aatgataaaa gcaggagaag gattagaaag atctggtttt
     4501 aatagtgctg gagcaaataa ataagaatag taatttaaaa ataactaaaa tgaatattaa
     4561 aaaaatgaat gttattgaat taaattccaa tgaatttaaa caagtaaatg gaggaaattg
     4621 gtatagatgg ggaataactt tattaggagc aatgattaat gatgctcaaa ataatcctga
     4681 tgactttgag gctggcatgg ctttttggga ataaatcaat cttttacatt aacaaagaac
     4741 gtgtaatttt tgtacgttct ttgttatgac acaccctatg tcataattat atctttttca
     4801 gttttttttg ggagggaggg ggaaatagca tatttttttg taaaaaaggt agttctccat
     4861 ttataacaat agtgatttta cactttttat ataaaattac tgtttatttc tttgaaaaaa
     4921 catatttcaa tattttaatt cttcaccgca atcgaattgc actaaacact cttttctttg
     4981 ctattctaaa ttgaaaaaga atggcaaaaa ttaccataaa acaacacgat attactgact
     5041 gtggtgctgc ttgtttagca tctatctcag cacattataa attacaactt cccattgcgc
     5101 gtatacgcca atatgcaggt accgataaaa aaggaaccaa tgtattagga ctcatagaag
     5161 ccgctgaaaa attaggtttt gaagccaaag gagtacgtgg tgaactcgat agtttgttta
     5221 aaatacctaa accagccata gcccatgtaa ttgttagaga acagttacag cattatgtgg
     5281 ttatttatga agtaactaaa gagtacatta aaattatgga tcctggtgat gggaaaatcc
     5341 ataaaaaaac acatgaagaa tttttaaaag aatggacagg aatactggta ttattattac
     5401 caaaagaaga attttcaaca ggtaatgaaa aagtgtctgt gctcaaacgt ttctggtttt
     5461 tgctaaaacc acacaagttt gttttattgc aagctttaat aggcgcagtt atttatactc
     5521 ttttaggctt ttcaacatcc atatatatac aaaaaattac agattatgta ttggttggcg
     5581 gtaatactaa gttgttgaac ttactcagta ttataatgct ttcgctacta gctttacaaa
     5641 ttattattgg agtatataaa gatgtgtttt taataaaaac tggtcagcaa atcgatgctc
     5701 gattaatttt aggctactac aaacatttat taaaactacc tcaacagttt tttgatacca
     5761 tgcgagtagg agaaattatt tctcgtatta atgatgctgt aaaaattaga acttttatca
     5821 acggagtgtc gctaagttta acagtaaaca ttcttatttt gtttttctct tttgggttga
     5881 tgttcatgta tcactggaaa ttagcgttaa ttatgttgtt ggtaattcct ttctatttaa
     5941 cagtatattt catcattaat aaactaaaca agaaaacaga acgtaaaata atggaacgtt
     6001 ctgctgattt agaaagtcag ttagtagagt ctttaaactc agtaggcacc ataaagcgtt
     6061 ttggtttaga aagttttgcc aatataaaaa cagaaacacg ttttataagt ttattacaca
     6121 taggttacaa atcggcttta aactcagttt tttctggaac ttcaactact tttatcgcac
     6181 agttgtttac tatagtttta ttatggagtg ggtcttattt tgtaattgaa aggcaaataa
     6241 ctgctggtga gttgatgtct ttttatgcta ttataggcta ctttatgaat cctgttgcta
     6301 gtttgatagg tgctaataaa caaatacaaa atgctttaat tgctgcagat cgtttgtttg
     6361 aaattatgga tttagagcgt gaagaaaaca cagaaaaagt agttcttaca aaagaaaaaa
     6421 taggagatat tgagtttaaa aatgtagctt ttcgttacgg aacaagagta caagttttta
     6481 cagattttaa tttaaaaatt aaaaaaggga atattaccgc cattgttggt gaaagtggct
     6541 caggaaaatc aaccttaatt tcactgttgc aaaacttata tcctatccaa aaaggacata
     6601 ttgcactggg agatttagac ttaaaataca ttcaacatga aagtttacgg gagttagtaa
     6661 gtgttgtacc tcaaaaaata gatttatttg caggaaatgt aattgacaat attgctgttg
     6721 gtgattttgc tcccaacatg gaattaatta ttgaaatttg taagtctata ggtattttag
     6781 agtttattga aagtttacct aatggttttg acacctattt aggagaaaac ggagctactt
     6841 tatctggcgg gcagaaacaa cgaatagcta ttgctagagc tttgtacaaa caacctgaaa
     6901 tactcgtttt agatgaagca acctcatctt tagattctac ttcagaaaac tatattcaaa
     6961 aagctgtaga aagattacgc caagaaaaca aaaccattat cattattgct catagattaa
     7021 gcactgtaat taatgctgat gaaattgtag ttctaaataa aggaaacgtc ttagagcaag
     7081 gaaaccataa agaactatat gttgctgaag gaaaatatta caatttatgg caacaacaaa
     7141 tgcctcaaat agaattaaac aaaaaagctc atcaaatttg atgagctttt tgaggtgtcg
     7201 agcggattcg aaccgctgta gatggtgttg cagaccactg cctagccact cggccacgac
     7261 accttatatt gataaggact gcaaatttac agcttttatc tttttttgca agcttttttt
     7321 actttttacg cacttctgat aaaacaattg caacctcagc aacatttccg ccaattggtg
     7381 gattaatttt tgctacgcta acctccgctt catcaaccaa ttgtatttct ttaaagattc
     7441 tatctaaaat tcgttgagca acatgctcta gtaatttaga acgaattgcc atctcttctt
     7501 ttacaatttt atttaaatgc acataatcta ccgtatcaac caaattatct gtttttgcag
     7561 atgtttgtaa atcagctttt acttcaatat caactctata ttccgaaccg attcttgctt
     7621 cttcatctaa gcatccgtga tttgtaaaaa cacgaatgtc gtttacacgt attattccca
     7681 taccgcaaaa atagtaatgt ttctttatta agttgtgtaa attttatttc aaaaaaagag
     7741 aaaaacagtt ttgtttttct cttttataat ctctttgtta aagaacttca aatgtttgac
     7801 ttaactcacc cactgttact ttatatttcc ctttaggtaa ataatacttg ttattctttg
     7861 ctttttttac cttagcttct ttgtttttct ttaagtactt ttttcttcct ttttcagaaa
     7921 aagaaacatc gtacactact tcattaaatc cttttgttgc tttagccttt atagtattta
     7981 ccaaaacttt acccgaatat atttctaaca caacttcttt atctgcattt acataaaaag
     8041 gaattgtgat ttttggtgta tttggttcta accatttact ccaagaacta ccccaagatt
     8101 ttcttttctt aatatccttc atactgaaaa tagcttcttt gttatcaaat tcttgtaaag
     8161 aagtaatatt cgctttgtac aaactacgcc catgcgttcc tactaataaa tgttttgctt
     8221 taggttgaat tactaaatcg tgtactgcaa catttggcaa tccgttgcta aaagcttccc
     8281 atgttgaacc ttgattgaaa gaaacataca atccgttgtc agttcctaag tataggacat
     8341 tctctttttt aggatcttcc ttaatcacat ttaccgcaga agctggaata ttactactta
     8401 tatttttcca tgtttttcca tagtcatcag acatgtatac atacggtgta aaatcatcaa
     8461 aacgatatcc gtttaacgta acatatacac gctctttttt atattttgaa gctattacac
     8521 gacttaccca taaattttgg ggtaaagaat tagagatttt ctcccaattt cctcctccgt
     8581 ttttagttac ttgtaccaat ccatcatcag tacctacgta gattaatcca aattgaaatg
     8641 gactttcaga aatagtagtt aatgttccgt aagctacatt tccttccttt tctcctttgg
     8701 ttaaatctcc agaaatttct tcccaatcat cacctttatt taaagaacgg tgtaacttat
     8761 ttcctcctaa gtataaaata tcttgattgt gttttgatag ctgaatagga gtttgccaat
     8821 tgaatcgata tgctggttta tctttttttg gtttcggctg aatataggtt tgctttcctg
     8881 ttgctctatc aattcgataa tagtttccaa attgataccc tgtatacaca atattcggat
     8941 ttctgtcatc aacagcaacc tgcattccgt cacctcccat taaactttcg taaggatttt
     9001 gtcctgtttg atgccagctt ttgtcaattt ttgcattgtg gtttcctacc caaactccgt
     9061 tgtcttgtaa tcctccatat accttgtaca cttcttgatt atccgcatat acggtataaa
     9121 actgtccaac tgctggttgg tttaatttaa tccagctctc accatcatcg tatgaaatat
     9181 ttaaccctcc gtcgtttcca tcaattaagt gtccttgttt tttagggttt acccatagcg
     9241 cctgatggtc tgcatgtaca ttttctttac taatagatgt aaatgttttt cctccatctt
     9301 tcgactttaa aattggtact cccaatacat agattccatt tttgtcttgc ggatcaacac
     9361 gaatttctcc aaaataatac ccgtacgaat agtataaatc atctaaatat ccttcatgtg
     9421 tttttttcca cgtttttcct ccatcaatag tcttaaaaac ctccgctcct attactggag
     9481 tatcaaataa cgctgcattt gcatctttta aataaccaat taaatcactt ggcttcattt
     9541 ctcccggact tgagcgaatg atttgcttaa tattttccgc acgatatttt tcttgaagac
     9601 cattcatttt gatatatgaa ttcaattttt tgtctttcat attcaaaagt tcatctgccg
     9661 aaattgatgc aagctcctct tttgttaagg ctcctgctcc cttctctttc ttagcaggat
     9721 ctttttttct tcggaattga ctatcgtgaa aagcatatac cgtgttttga tcatatactg
     9781 ctaaaccaat tcttcctact ccatctccat tagggaaacc attattatca gaaattttag
     9841 tccatgtact cccagcatct gtcgatttat aaattgcaga attttttccg tctccatcaa
     9901 aattccatgc tttacgttca cgctcccaag cagcagcata taaaatattg aagttatttg
     9961 gtacaggttg aatatctata attccagtat tttcatcaat aaacaaggtt tttatccagg
    10021 tttttcctcc atctgtagtt ttaaaaattc ctctttcatt gtttgatgaa tataaatgcc
    10081 ctattgaacc tactattact tcatcaggat tttttggatt gattaaaatt cgtccaatat
    10141 gatgtgaatc gagtaaccca acgtttgtcc aagttttacc tttatcggta gattttaaaa
    10201 ttccgattcc tgcatacgat gatcgagaag aattattttc tcctgtacca acccaaattg
    10261 ttccattttt ccaatctaca gcaatatcgc ctacgttttg tgtaggagag ttgtctagaa
    10321 ttggagtaaa agttgttccg ttattatcag tatgccatac tcctcctgaa gcataaccta
    10381 cataaaactc tgtagtattt gctgggttta catcaatatc cactacacgc ccactcatca
    10441 ctgtaggtcc tatattttta aagggaatat tttttactgt agaagttaaa gttaatgctt
    10501 ctttttgttg taaagcttgt tgtattttct ctgcagatgt aggttgctgt gcagctactg
    10561 taaacggaaa taaagctatt aaagctttgg ctaaggttct cattataatt tggtttagaa
    10621 gaagtgaaaa tactgtaagc aaaaactatt tcaaaaaaat taacagaata ttgagagact
    10681 tctttttgaa gttcttgtca aacaaaaaaa ttagtagctt tgtttaacct caaaattttt
    10741 aggttatgac tatttttatt ctttctgtta gtgaccagat ttattcaaca caaagaatat
    10801 ataaagaggc aagtcgtcgt ggacataaag ttgaaattat aaaccattta aaatgtttta
    10861 taaaacttgg cgatggtaaa cctgctattc tatacaacgg taaaaatatt attgatattc
    10921 ctgatgttat tattcctcga ataggtgttt cagccatgaa acatggaaca acagttgtaa
    10981 aagagtttga aatgaacgga gtatatagta ctgctagtta tttaggtatt gttgatgcac
    11041 agaacaaagc tcgtacttta caaataatga gtagaaatgg acttcctatt ccgcagactg
    11101 ttttttctgt tgacgctgaa aatattggtg agcaaataga tttattaggt ggttctcctg
    11161 taattattaa gctacaagaa ggaacacatg gctctggagt aattttagct gaaagcaaaa
    11221 aatctgcgat gtctattatt gatactatgc acggaacaaa taccagtatt ttactacaag
    11281 aatttattca ggaaagtaac agtgaagata ttcgtgcttt tattgtaaat ggcaaagtgg
    11341 tatcttctat gaaacgtaaa gggctcgagg gggactttcg ttctaacatt cataaaggag
    11401 gtagcggaca caaggttaca ttaactcccg aagaagaaga aatagctatt aaagcagctc
    11461 aacacttaga acttcctatt gctggcgttg atatgattcg atcaaaaaga ggacctctat
    11521 taattgaagt caactcaact cctggcttac aaggaattga atcctacacc aaagacaata
    11581 ttgctaaagt tattattaaa tttttagaag aaaatgttcg aacaaaattt taaaaacgaa
    11641 atcatagaaa ttcgtggaca aaaaattggt ttaggcgaat ctaaattaat taagattcca
    11701 attgataggt tacctacagg tactctaata gaaattcctg tgtatatttt taatggtaat
    11761 caattaggtc ctactattct actacagggt ggtctacacg gagatgaagt aaatagtatt
    11821 gaattagtca gaagaatgtt aattgataaa tcgtacaaaa ttcaccgagg ttgtgttatc
    11881 gtggttcctt tacttaatgt gtttggcttt ttaaatatgt cgagagacat gcatggtaaa
    11941 gatgtaaatc gtagtttccc tggttcaaaa acgggttctt tagctagtag aatggcgtat
    12001 tatttaatga aagaaattac caataatgta gattttgcta ttgattttca tacgggaggt
    12061 gaacaacgaa ataacttccc acaaatacga tacactcctg aagataaaaa aggagttcaa
    12121 ctagccaata ttttcaatgc tccatttatg tttggctcta aacttattcc gaagtctttt
    12181 a
//
