LOCUS       NZ_JAMTCR010000002     10378 bp    DNA     linear   CON 29-AUG-2024
DEFINITION  Actinokineospora globicatena strain DSM 44256
            LV79DRAFT_scaffold00001.1, whole genome shotgun sequence.
ACCESSION   NZ_JAMTCR010000002
VERSION     NZ_JAMTCR010000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinokineospora globicatena
  ORGANISM  Actinokineospora globicatena
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Actinokineospora.
REFERENCE   1  (bases 1 to 3058629)
  AUTHORS   Goeker,M.
  TITLE     Genomic Encyclopedia of Archaeal and Bacterial Type Strains, Phase
            II (KMG-II): from individual species to whole genera
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3058629)
  AUTHORS   Goeker,M.
  TITLE     Genomic Encyclopedia of Archaeal and Bacterial Type Strains, Phase
            II (KMG-II): from individual species to whole genera
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3058629)
  AUTHORS   Goeker,M., Huntemann,M., Clum,A., Wang,J., Palaniappan,K.,
            Ritter,S., Chen,I.-M., Stamatis,D., Reddy,T., O'Malley,R., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2022) Prokaryote Super Program, DOE Joint Genome
            Institute, 1 Cyclotron Road, Mail Stop: 91R0183, Berkeley, CA 94720,
            USA
REFERENCE   4  (bases 1 to 3058629)
  AUTHORS   Goeker,M., Huntemann,M., Clum,A., Wang,J., Palaniappan,K.,
            Ritter,S., Chen,I.-M., Stamatis,D., Reddy,T., O'Malley,R., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2022) Prokaryote Super Program, DOE Joint Genome
            Institute, 1 Cyclotron Road, Mail Stop: 91R0183, Berkeley, CA 94720,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: AllPaths v. r46652
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 261.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024171945.1-RS_2024_08_29
            Annotation Date                   :: 08/29/2024 06:43:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,731
            CDSs (total)                      :: 6,666
            Genes (coding)                    :: 6,609
            CDSs (with protein)               :: 6,609
            Genes (RNA)                       :: 65
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 8 of 57
            Pseudo Genes (incomplete)         :: 49 of 57
            Pseudo Genes (internal stop)      :: 2 of 57
            Pseudo Genes (multiple problems)  :: 1 of 57
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:43:01
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2966988
            Orig. end                         :: 2977366
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAMTCR010000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10378
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="12"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10378
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10378
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5378](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5378
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    222..242
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.12"
                     /tool="antismash"
     misc_feature    complement(838..853)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 22.0"
                     /tool="antismash"
     misc_feature    complement(1092..1107)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 22.73"
                     /tool="antismash"
     misc_feature    1752..1767
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.91"
                     /tool="antismash"
     misc_feature    complement(2884..2899)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.69"
                     /tool="antismash"
     gene            3234..4706
                     /locus_tag="LV79_RS13160"
                     /old_locus_tag="LV79_002572"
     CDS             3234..4706
                     /NRPS_PKS="Domain: AMP-binding (11-390). E-value: 6.9e-73.
                     Score: 237.5. Matches aSDomain:
                     nrpspksdomains_LV79_RS13160_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012711.5"
                     /locus_tag="LV79_RS13160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LV79_002572"
                     /product="AMP-binding protein"
                     /protein_id="WP_253835463.1"
                     /sec_met_domain="AMP-binding (E-value: 6e-74, bitscore:
                     246.3, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWSSDLLRAVRAHAGGERTAVYDRGRAWSYRQLWETARGVAGELA
                     AVGVVPGRPVLVNAKKSAGAVALVLGCGIAGVPAVLVSVDLGADAVELLVERTGSTHVL
                     GVDVEPEPGAVVGLRARATGVAVAEAMPGDPPLVLTTSGSTGAPKVVPLSRVGVERFTD
                     WAATTFGIGAGTVVLNYAPLNFDLTLLDIWATLVAGGSVVLVDPDRAADGRHLAELVAA
                     HQVEVVQAVPLCLRLLAAQEQRFESVRQVITTGDVLPSAVLATFPTVFPNAAVHNLYGC
                     TETNDSFLHTVTDFDTTAPLPVGRPIDGVRALVLDPDGTEILGAGRGELLVSTPFQADG
                     YLDPDLTAARFVTGVAGHDGVFYRTGDIVRRDADGLLHLDGRDDFHVKVRGVRTNLQEV
                     EHVLDQHPDVSEVAVVAIPDLEAGLRLHAAVRVDSDINSLALRAHCAKSLPRTAIPSSF
                     EIQQSALPRTSTGKVDRNAISRSHQEARKAHV"
     aSDomain        3267..4403
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LV79_RS13160_AMP-binding.1"
                     /evalue="6.90E-73"
                     /label="LV79_RS13160_AMP-binding.1"
                     /locus_tag="LV79_RS13160"
                     /protein_end="390"
                     /protein_start="11"
                     /score="237.5"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="AHAGGERTAVYDRGRAWSYRQLWETARGVAGELAAVGVVPGRPVL
                     VNAKKSAGAVALVLGCGIAGVPAVLVSVDLGADAVELLVERTGSTHVLGVDVEPEPGAV
                     VGLRARATGVAVAEAMPGDPPLVLTTSGSTGAPKVVPLSRVGVERFTDWAATTFGIGAG
                     TVVLNYAPLNFDLTLLDIWATLVAGGSVVLVDPDRAADGRHLAELVAAHQVEVVQAVPL
                     CLRLLAAQEQRFESVRQVITTGDVLPSAVLATFPTVFPNAAVHNLYGCTETNDSFLHTV
                     TDFDTTAPLPVGRPIDGVRALVLDPDGTEILGAGRGELLVSTPFQADGYLDPDLTAARF
                     VTGVAGHDGVFYRTGDIVRRDADGLLHLDGRDDFHVKVR"
     CDS_motif       3645..3695
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LV79_RS13160_0001"
                     /evalue="2.70E-04"
                     /label="NRPS-A_a3"
                     /locus_tag="LV79_RS13160"
                     /protein_end="154"
                     /protein_start="137"
                     /score="13.9"
                     /tool="antismash"
                     /translation="VLTTSGSTGAPKVVPLS"
     CDS_motif       4215..4286
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LV79_RS13160_0002"
                     /evalue="1.40E-06"
                     /label="NRPS-A_a6"
                     /locus_tag="LV79_RS13160"
                     /protein_end="351"
                     /protein_start="327"
                     /score="20.7"
                     /tool="antismash"
                     /translation="GELLVSTPFQADGYLDPDLTAARF"
     CDS_motif       4374..4433
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LV79_RS13160_0003"
                     /evalue="5.20E-05"
                     /label="NRPS-A_a8"
                     /locus_tag="LV79_RS13160"
                     /protein_end="400"
                     /protein_start="380"
                     /score="15.9"
                     /tool="antismash"
                     /translation="GRDDFHVKVRGVRTNLQEVE"
     gene            4699..4962
                     /locus_tag="LV79_RS13165"
                     /old_locus_tag="LV79_002573"
     CDS             4699..4962
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1147: putative acyl carrier protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680900.1"
                     /locus_tag="LV79_RS13165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LV79_002573"
                     /product="acyl carrier protein"
                     /protein_id="WP_253835465.1"
                     /transl_table=11
                     /translation="MSETHAIKVYITTSFAPDVSPDELGDDYDLLANGVVESLHLLRLI
                     AWLGERFEVNLEDADVAPEDFRTVATIHRTIAELRGVATAAR"
     gene            5001..5378
                     /locus_tag="LV79_RS13170"
                     /old_locus_tag="LV79_002574"
     CDS             5001..5378
                     /GO_function="GO:0033990 - ectoine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645375.1"
                     /locus_tag="LV79_RS13170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LV79_002574"
                     /product="ectoine synthase"
                     /protein_id="WP_253835467.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.8e-28, bitscore:
                     96.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLIRHKDTVNTVDWGNGLSHRLLVESDGMGFAMAHTVVAAGTSSR
                     LQYRQHLEACYCVSGKGEVHSADGSVRQIIEPGVLYALDQHDAHTLIAASGDDMHLISV
                     FNPPIRGDERHRLDPDGYSQY"
     misc_feature    5064..5066
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5451..6593
                     /locus_tag="LV79_RS34440"
                     /old_locus_tag="LV79_002575"
     CDS             5451..6593
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646413.1"
                     /locus_tag="LV79_RS34440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LV79_002575"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_253835469.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 1.1e-16, bitscore:
                     58.8, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 3.7e-27, bitscore:
                     91.7, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 2.1e-31, bitscore:
                     106.5, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDWNEDQKLFRKTIEELGPVLSDGHVEADRDGEFSRAKWATLAA
                     SGLFGLPFDEAFGGLGQDVPTTMYVLEGLGHANRDGGLSFSASTHLASTGTPLNRFGKP
                     ALKERLLPAVCAGEIIGAHAITEPAHGSDAMGMQTTAVPDGDHYILNGSKAFVSNGPVA
                     DVVVVYARTGNPGTASGISAFLVERGTPGLSFGNPIEKMGLRSSPLCELFLDNVRVPAE
                     NLLGRPGNGFLVLDHVMKREILYGFAINLGEMQYRLEQATSYARERTQFGSPIGSFQAV
                     QHRVVDMLIGTETARHWLYSTGRKLAQGKDVTTDVAITKLVVSEAALASALNTVSVFGG
                     YGYMAEYGFEKDVRNAVAGTIYSGTTEIQKSRVAAMLGLG"
     gene            6604..7278
                     /locus_tag="LV79_RS13180"
                     /old_locus_tag="LV79_002576"
     CDS             6604..7278
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646412.1"
                     /locus_tag="LV79_RS13180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LV79_002576"
                     /product="transglutaminase family protein"
                     /protein_id="WP_253835471.1"
                     /transl_table=11
                     /translation="MTTASALLAATDLLDHESDEVRRFVAAAVPDPGVSDREKAVALFY
                     AVRDGVFYEVYGADMSHEGLRASSVATAKTGFCLHKSILYAAATRAVGIPSRVLAGEVR
                     NHLASDKLKDLVGGEVFLHWLNEVWLDGRWLKTTPVFNRMLCKLYGIAPLEFDGTADAV
                     YHPYDEQGRQHMEFVGAVASFNDVEHAAVIDLMRGRHPNMFASATAVRSGGSLVDEAPT
                     RG"
     misc_feature    6796..6798
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(7294..7307)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.26"
                     /tool="antismash"
     misc_feature    7298..7311
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: strong, score: 19.16"
                     /tool="antismash"
     gene            7348..7956
                     /locus_tag="LV79_RS13185"
                     /old_locus_tag="LV79_002577"
     CDS             7348..7956
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013488.5"
                     /locus_tag="LV79_RS13185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LV79_002577"
                     /product="DsbA family protein"
                     /protein_id="WP_253835474.1"
                     /transl_table=11
                     /translation="MSPPEITVYFDYVCPYCLLAEDTIADAAAETGAEVSWRAFELRPH
                     PNPTLRPEDDYLPRVWRQSVYPMAERLGVPISLPTISPQPYSHLAFEGAHFAKAHGSDG
                     AYHAAVLRAFFQQDLDIGRPEVLAGIAGDIGLDTAEFTLALKDRRYAEDHRTELAAAYR
                     AEVHAVPTIDVGSRRFTGVPDPRELRAAIAALSADQQAQ"
     gene            7977..8294
                     /locus_tag="LV79_RS13190"
                     /old_locus_tag="LV79_002578"
     CDS             7977..8294
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073952.1"
                     /locus_tag="LV79_RS13190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LV79_002578"
                     /product="multidrug efflux SMR transporter"
                     /protein_id="WP_253835476.1"
                     /transl_table=11
                     /translation="MQWVYLGIATVFEIAFALAANASKGFTRLWPTILTLVIGALGTFF
                     LSLALRTLDIGVGYAIWTGLGSVGIVLLGSLVFKERIDWRKALGIAVVIVGVVGLELSG
                     V"
     gene            8324..8722
                     /locus_tag="LV79_RS13195"
                     /old_locus_tag="LV79_002579"
     CDS             8324..8722
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419417.1"
                     /locus_tag="LV79_RS13195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LV79_002579"
                     /product="multidrug efflux SMR transporter"
                     /protein_id="WP_253835478.1"
                     /transl_table=11
                     /translation="MTASSTELAQQGSDTAQPTARGWLVLLVAGIFEIGYAVSVGGSKG
                     FTDLNWSLSAGVFFFLTVFTLTVALKYLDVGIGYAVWTGIGASGAAVASALIFDQPLTT
                     TRVLWLAVIIAGVVWIKLASSPKHAEQA"
     gene            8719..9192
                     /locus_tag="LV79_RS13200"
                     /old_locus_tag="LV79_002580"
     CDS             8719..9192
                     /EC_number="3.5.4.33"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_function="GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1240: riboflavin biosynthesis protein RibD"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF025793.5"
                     /locus_tag="LV79_RS13200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LV79_002580"
                     /product="nucleoside deaminase"
                     /protein_id="WP_253835480.1"
                     /transl_table=11
                     /translation="MTVLDDAQITEYLREAVALSQEEVDRGGIPFAGLLARPGAGVLGT
                     GVNRVLQRRDPTAHAEIVALRDAAANHDGAAIAESVLFASGEPCGMCYQAAIDAGITSV
                     VYAVGADDAARYGFDYRASYRHVDRTDGGRVTCRSWPVDQALTPFTTWADRNL"
     gene            9221..10024
                     /locus_tag="LV79_RS13205"
                     /old_locus_tag="LV79_002581"
     CDS             9221..10024
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942695.1"
                     /locus_tag="LV79_RS13205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LV79_002581"
                     /product="maleate cis-trans isomerase"
                     /protein_id="WP_253835482.1"
                     /transl_table=11
                     /translation="MTTQISPSPGRWVDDGWNTTVRMGVVVPHADVGPENELRAMAPDH
                     LSVHAGRLHFSAMRAGGEMDPTIPHDPVRAFTEPPHLDNCVEQLAQAPLDVIGCAFTSS
                     AYKHGPRGEHALAARLAEHARGMPVTTTCLAAEQAIRALGVDRLALVNPPWFDEELSAA
                     GAAYFTALGVDVVHHGACGLRSGQPHITPPALYDWIRSVAGGASAVFVAGNGMRAVGII
                     SALEDRLGIPVLTANQVLLWHVMELTGTTVSRPGYGKLMNMYPST"
ORIGIN
        1 aaaggtcgcg ccgctgttcg gcgtcccctt cgagcgcaac ccgttccgcc cgctgacctg
       61 ggtctgcgac ttcaccgcga tcaccgtctc ggagatcgcg cgcggcgcgc cgctgccgac
      121 cagggccgac gcggcccggt tgcgcggcac cgagcacgag ggcgagtggt tcgtgcacga
      181 ccgcgcggtg ctgccgggcg atcggggcgc gctgcccaac gagatcgtcg cggcgacggt
      241 caaccggttc ggccccgaca ccaaggccgc cgtcgtgctc accgcgacca acgtgctgct
      301 cgccccggcc accgaggcca tccggaccgc gctggggatg ctgcgcgcgc tcgacggcgg
      361 tgacctcccg caggtgcagt ggatcgccgc gtgggcggcg accgcgatcg aggtgtaccg
      421 gtcgcagccc gcgctggtgt ccgcggcgct caccgcccgg gtgatccagc gcgcgtcgct
      481 cgccgcgccg aggttcccgc tcgcgccggg gatccacggg cgccccaggg cgcggtgcga
      541 gatcggcgcc acgatcccgg agtccactgt ggacccggtg acccggccga gggacctggg
      601 gttcctcgac gggatcgcgg ccgcccggct caacgccgcg gggacgctgc ccgaggtcgg
      661 caccgaggaa ccgaccggta tcggcgcggg cgtcggcgac cggctggtcg agcagttgat
      721 cgggttgctg gtggacatgg gcacccccga cggggtggcg cacatctggg tcagcgagcg
      781 ggtcgcgggc caggaacagg tgtgggccat ggtcccgtcc tccggcctgg tccgcgaact
      841 ggtcgagacc tgggcgcacg ggcgcgggtc gttgccgggg gcgtccgggg gcgccgacgc
      901 gctggccacc gcgatggcgg tgccgttccg gctgccgctg cccaccgagc tcgcctcggt
      961 cgacctgctc gccaggcgcg ccttcgtgct ggcggtgatg gggatcatcc ggcagatggg
     1021 cctgctggcg cagtcgacct ggttggcggg cgaggagttc accgacctgg tacgcgggtt
     1081 ggaccgcctg ctcgacgagg cattcgacca ggacgacccg gtcgtggtcg acaccaggct
     1141 gcggttggcg gtgcagcgcg caggggccga gcggcacgcc gggcgcagca cacccgaggc
     1201 cgccgcggcg gtcatcgcgg cggcggacgc ctgcgtcgcg gcgggcgcgg ccgaccggat
     1261 cgaccccggg ctgctcgccg acctgttggc ggtggcctgc atcgagctca actcgctgcg
     1321 ctccaccggc ctgcgccgga tgggcgtcgg cgccgcgctg cgccggtact gggccgcgtt
     1381 cgcggccacg gtcgaggtcg acctgttcga gccgggggcc gatcacggcg gcctgagctt
     1441 ccagttgcac aactacgcgg ccttcctggg ggccaacaag gacaacgccg aggacctgcg
     1501 cgccgccctg cacctgttca cgtcttcggt catccctggg cggtcccggc tctacaaccg
     1561 ggaccgcgac atccgcccgc tggcccgcag ccgctacctc gccgcggacg ccgccgccgg
     1621 actcgcctca ctgctgacgg acagcggcga gcacgccgcg gcccagcagt gggcgcgcca
     1681 agcccacacc tgggtgcacc aggtgctggc cgacccgctc taccagccgc accacctgcg
     1741 acccgagctc gacgactgcc tgttcgcgct gcgcgtcgcg ccggtccttg tgctggcgat
     1801 cgaactcggt gtcgccgacg agcccgagtc ggacgcgacc acggccgacg cgttggcgcg
     1861 cctagtcgag cggtggttgc gggaggcgac caacggcgac atcgagcgat ccgaccacct
     1921 gcccgccgtc gtcgacctgc gcacgcggct caccgcactc ggtttcccca gctgatccca
     1981 cccgcgcccc tctcctggcg ttcggctcca ccgcacccgc tgacgaccac cacgcccgcg
     2041 ccacccccaa ccggaccgtc aatgtccggt cggaggtgat cagccatgcg aaccggtggc
     2101 ggaaacggcg acgacagcaa gtgacaacgg agaacccgaa acagcaccac atttccacgc
     2161 ggtgatcggg atggggcggt ggcatcaaca ccacccatcc cgaccacccg cgcgtttcga
     2221 cttgtggaca cccgccccac ggcgagcacc gcgatcacca catcggtccg aacccgcgaa
     2281 gtgctacatc gggtttcgcg ctgccagtgg tcagtggcaa gccgccacct gggttcgact
     2341 gcccactgtg gccggtcgtg ggcgatcggg ttgacagaaa caggacagcg cggcccaatc
     2401 cccggttgac cgcattgggt gggcccagtc gatatgaccc gtgctcgtgt tcgcgccgag
     2461 ttcggattat cgatcaagtg gtgacgagcg cattgtccgg ttcgtgggcg gaccgcctgg
     2521 ggcgcggcca cgcctattcg cccatggccg tcgcggccga agcggaaccg gattcgcctc
     2581 gcgacgaatg tcgctgaaat cgaactgggg aacgcctgcg cggccgctgg tatgggtcag
     2641 ccgccaatcg ttcgacgcct gggtcgcgtt aggtctgatt ggccaaaatt cctgtggggt
     2701 ccatcacgcc ctctttgggg tggcgtgcct cggcgcgtcc acaaaggatg accgctggcc
     2761 ggacggtggt gttcgcgctg gaaaggtgag gtggttccgg tttggggggt gtgcggtttg
     2821 tgtggttcgg ggtgggtagt tcgctattag ggaaactaac gaataagggt gagtggtggg
     2881 ataggtggtc tacccctagg tcgggtgggg ttgcttgaca tgggcgtgga aaccatttga
     2941 cgttgagttc agtgggtgtt tacgaattct gccggttcgg cacgctgcga actcgggatg
     3001 gtcgcacgtg gtgatactgg ctgctcaggg ttggtatttg taggaaccgt cagtggttgg
     3061 tgggtttccc actttgatcc gttcagggta ttgcgcgaag tacttatatg cgcaaacatg
     3121 gaccgtgcct ggggcgttgc tggtgtgggt cgcgatgccc ggggcggggg tcccatctca
     3181 tcgcagtctc tttggctctc agtcggcctg tgtgtggaaa ccgagggaga actatgtggt
     3241 cgtccgacct gttgcgtgct gtgcgtgcgc acgccggggg tgagcggacc gcggtctacg
     3301 accgtggacg tgcgtggtcc taccggcagc tgtgggaaac ggcgcgtggc gtcgcggggg
     3361 aattggccgc ggtcggggtc gtgccgggac gtccggtgct ggtcaacgcg aagaagtccg
     3421 ccggtgcggt cgcgctggtg ctcgggtgcg ggatcgccgg ggtgccggcg gtgttggtct
     3481 cggtcgatct cggggccgac gcggtggagt tgctggtgga gcgcacgggg agtacccacg
     3541 tgctcggcgt cgacgttgag ccggaacccg gtgccgtcgt gggacttcgc gcgcgggcca
     3601 ccggagttgc ggtggcggag gccatgcccg gtgatccgcc gctggtgctg acgacgtccg
     3661 ggtcaaccgg ggcgccgaaa gtggtgccgc tgagccgggt cggtgtggaa cggttcaccg
     3721 actgggcagc cacgaccttc ggcatcggcg cgggtacggt ggtgctcaac tacgcgccgc
     3781 tgaacttcga cctgacgttg ctcgacatct gggcgacact ggtcgcgggc ggttccgtgg
     3841 ttctcgtcga cccggaccgt gccgccgacg gtcgacacct ggcggaactc gttgccgcgc
     3901 accaggtcga ggtcgtgcaa gcggtaccgt tgtgcctgcg cctgctcgcc gcgcaggagc
     3961 agcggttcga gtccgtgcgg caggtgatca ccaccggcga cgtcctgccc tcggccgtgc
     4021 tcgccacctt ccccaccgtc ttcccgaacg cggccgtcca caacctctac ggctgcaccg
     4081 agaccaatga cagcttcctg cacaccgtca ccgacttcga caccaccgcg ccgctgccgg
     4141 tcggccgtcc gatcgacggg gtccgcgcgc tggtcctcga tcccgacggc accgagatcc
     4201 tcggcgcggg tcgcggcgaa ctgctggtgt ccacaccgtt ccaagcggac ggttacctcg
     4261 accccgatct gaccgccgcg cggttcgtca ccggcgtcgc cgggcacgac ggtgtcttct
     4321 accgcaccgg tgacatcgtc cgccgcgacg ccgacggcct gctgcacctc gacggccgcg
     4381 acgacttcca cgtcaaggtg cgcggcgtgc gaaccaacct gcaagaggtc gagcacgtgc
     4441 tcgaccagca cccggatgtc tccgaggtcg ccgtggtcgc gatcccggac ctcgaggcgg
     4501 gactgcggtt gcacgccgcg gtgcgcgtcg acagtgacat caacagcctc gcgctgcgcg
     4561 cgcactgcgc caagtcgttg ccgcgcaccg ccatccccag ctcgttcgag atccagcagt
     4621 ccgcgttgcc ccggacctca accgggaagg tcgaccgcaa cgcgatcagc cggtcacacc
     4681 aggaggcaag gaaagcccat gtctgagacc cacgcgatca aggtctacat caccaccagc
     4741 ttcgccccgg acgtctcgcc cgacgagctc ggcgacgact acgacctgct ggccaacggc
     4801 gtggtggaga gcctgcacct gctgcggctc atcgcctggc tgggcgagcg cttcgaggtc
     4861 aacctggagg acgccgacgt cgcgccagag gacttccgca ccgtggccac catccaccgc
     4921 accatcgccg aactgcgcgg cgtcgcgacc gcggcccgct agaacccctg ccgcacaaca
     4981 actctcagtg gaggttaccg atgctcatcc gccacaagga cacggtcaac accgtcgact
     5041 ggggcaacgg cctcagccac cgattactgg tcgagtccga cggcatgggg ttcgccatgg
     5101 cgcacaccgt ggtcgccgcg ggcacctcgt ctcggctgca gtaccgccag cacctcgagg
     5161 cctgctactg cgtgtccggc aagggcgagg tgcactcggc cgacggctcg gtccgccaga
     5221 tcatcgagcc cggcgtgctc tacgcgctcg accagcacga cgcgcacacg ctgatcgccg
     5281 cctcgggtga cgacatgcac ctgatcagcg tgttcaaccc gcccatccgg ggcgacgagc
     5341 ggcaccggct cgaccccgac gggtactcgc agtactgagc ccgccgccac cgaggacgcg
     5401 ctcctggtgt gcggcccggc cgcacaccag gacgacggag aggtccgcac gtgactgact
     5461 ggaacgaaga ccagaagctg ttccgcaaga cgatcgagga gttgggcccg gtcctgtccg
     5521 acgggcacgt cgaggccgat cgcgacggcg agttctcccg ggcgaagtgg gccacgctcg
     5581 ccgcgtccgg gctgttcggc ctgcccttcg acgaggcctt cggcggcctc ggccaggacg
     5641 tgcccaccac gatgtacgtc ctggagggcc tgggccacgc caaccgcgac ggtgggctga
     5701 gcttctccgc ctccacccac ctggccagca ccggcacacc gctcaaccgg ttcggcaagc
     5761 ccgcgctcaa ggaacgcctg ctgcccgcgg tgtgcgcggg ggagatcatc ggcgcgcacg
     5821 ccatcaccga gcccgcccac gggtccgacg ccatgggcat gcagaccacc gccgtacctg
     5881 acggcgacca ctacatcctc aacggcagca aggctttcgt cagcaacggc ccggtcgcgg
     5941 atgtggtcgt cgtctacgcg cgcactggga atcccggtac cgcgtctggc atcagcgcgt
     6001 tcctcgtgga gcgcggtaca ccggggctga gcttcggcaa ccccatcgag aagatgggtc
     6061 tacgctcgtc gccgctgtgc gagttgttcc tggacaacgt gcgcgtgccc gccgagaacc
     6121 tgctcggcag gccgggcaac ggtttcctgg tgctcgacca cgtgatgaag cgcgagatcc
     6181 tctacgggtt cgccatcaac ctcggcgaga tgcagtaccg gctcgagcag gcgacgtcct
     6241 acgcccgcga gcgcacccag ttcggctcgc cgatcggttc gttccaggcc gtgcagcacc
     6301 gcgtggtcga catgctcatc ggcaccgaga ccgcgcgcca ctggctctac tccaccggcc
     6361 ggaagttggc gcagggcaag gacgtcacga ccgatgtcgc catcaccaag ctggtggtca
     6421 gcgaggccgc cctggccagc gcgctgaaca cggtgagcgt gttcggcggc tacggctaca
     6481 tggccgagta cggcttcgag aaggacgtcc gcaacgccgt cgccgggacc atctactccg
     6541 gcaccaccga aatccagaag agccgcgtcg cggcgatgct cgggctcggc tgagggagaa
     6601 gacatgacca ccgccagcgc gttgttggcc gccaccgacc tgctcgacca cgagtccgac
     6661 gaggtccgcc gcttcgtcgc cgccgcggtc cccgaccccg gcgtctccga ccgggagaaa
     6721 gccgtcgcgc tgttctacgc cgtgcgcgac ggcgtgttct acgaggtata cggcgcagac
     6781 atgtcccacg agggcttacg cgccagctcc gtcgccactg ccaagaccgg gttctgcctg
     6841 cacaaatcca tcctctacgc ggcggcgacc cgcgcggtgg gcatccccag ccgggtgctg
     6901 gccggtgagg tgcgcaacca cctcgcctcg gacaagctca aggacctggt gggcggtgag
     6961 gtgttcctgc actggctcaa cgaggtctgg ctcgacggtc gctggctcaa gaccaccccg
     7021 gtgttcaacc ggatgctctg caagctctac ggcatcgcgc cgctggagtt cgacggcacc
     7081 gccgacgccg tctaccaccc ctacgacgag cagggccgcc agcacatgga gttcgtcggc
     7141 gcggtggcct cgttcaacga cgtggagcac gcggcggtca tcgacctcat gcgcggcagg
     7201 cacccgaaca tgttcgcctc cgccacggcc gtgcgctccg gtgggtccct tgtggacgaa
     7261 gcgcccactc gcgggtgatc agtacttgac aagagcatgt atatgcatgc aagtattgtc
     7321 ccggccgagc cggacagggg agtgagcatg agcccgcccg agatcaccgt gtacttcgac
     7381 tacgtgtgcc cgtactgcct gttggccgag gacaccatcg ccgacgcggc ggccgagacc
     7441 ggcgccgagg tcagctggcg cgccttcgag ctgcgcccgc acccgaaccc gacgctgcgc
     7501 ccggaggacg actacctgcc ccgggtctgg cgccagtcgg tgtacccgat ggccgagcgg
     7561 ctcggcgtgc cgatctcgct gccgaccatc tcgccgcagc cctactccca cctggccttc
     7621 gagggcgcgc acttcgccaa ggcgcacggc agcgacggcg cctaccacgc cgcggtgctg
     7681 cgcgcgttct tccagcaaga cctcgacatc ggccgacccg aggtgctcgc gggcatcgcc
     7741 ggggacatcg gtctcgacac cgcggagttc accctcgcgc tcaaggaccg ccgctacgcc
     7801 gaggaccacc gcacggagct cgcggccgcc taccgggccg aggtgcacgc cgtgcccacc
     7861 atcgacgtcg gctcccggcg gttcaccggc gtgccggacc cgcgggagct gcgcgccgcc
     7921 atcgccgccc tgtctgccga ccagcaggcc cagtaagaac acgagaggac aacgtcatgc
     7981 agtgggtcta cctcggcatc gccacggtgt ttgagatcgc cttcgccctg gccgccaacg
     8041 cctccaaggg cttcacccgg ctctggccga ccatcctcac cctggtcatc ggcgcgctcg
     8101 gcaccttctt cctgagcctg gcgctgcgca ccctcgacat cggcgtcggc tacgcgatct
     8161 ggaccggcct cggctcggtc ggcatcgtgc tgctcggctc gctggtgttc aaggagcgca
     8221 tcgactggcg caaggccctc ggcatcgccg tggtcatcgt cggcgtcgtc ggcctcgagc
     8281 tcagcggcgt ctgagccccc cactcggact taaggagttc accatgaccg cttcctcgac
     8341 cgaactggcg cagcagggct ccgacacggc ccagcccacc gcccgcggct ggctcgtgct
     8401 gctggtggct gggatcttcg agatcggcta cgccgtgagc gtgggcggca gcaagggctt
     8461 caccgacctc aactggtcgc tgtcggccgg tgtcttcttc ttcctcaccg tgttcacgct
     8521 gaccgtggcg ctcaagtacc tcgacgtggg catcgggtac gcggtgtgga ccggcatcgg
     8581 cgcctcgggc gccgccgtgg ccagcgccct gatcttcgac cagccgctga ccacgacccg
     8641 cgtgctgtgg ctggccgtca tcatcgccgg tgtcgtctgg atcaagctcg cctccagccc
     8701 gaagcacgcc gagcaggcat gaccgtgctc gacgacgcgc agatcaccga gtacctgcgg
     8761 gaagcggtcg cgctctcgca ggaggaggtc gaccgcggcg ggatcccgtt cgccgggctg
     8821 ctcgcccgac ccggtgccgg ggtcctcggc accggggtga accgggtgct ccagcggcgc
     8881 gacccgaccg cccacgccga gatcgtcgcc ctgcgcgacg ccgcggccaa ccacgacggg
     8941 gcggccatcg ccgagtctgt gctcttcgcc tccggcgagc cgtgcggcat gtgctaccag
     9001 gccgccatcg acgcgggcat cacctccgtc gtctacgccg tgggcgccga cgacgcggcc
     9061 cgctacgggt tcgactaccg ggcctcctac cggcacgtcg accgcaccga cggcggccgg
     9121 gtcacctgcc ggtcctggcc cgtggaccag gcgctgaccc ccttcaccac ctgggccgac
     9181 cgcaacctgt gaccacccga cccacccggg agcgcacgcg atgacgactc agatctcccc
     9241 gtcccccggc cgctgggtcg acgacggctg gaacaccacg gtccggatgg gcgtggtcgt
     9301 gccgcacgcc gacgtcggcc cggagaacga gctgcgggcg atggcccccg accacctgtc
     9361 cgtgcacgcc gggcggctgc acttctcggc gatgcgcgcg ggcggcgaga tggaccccac
     9421 catcccgcac gacccggtgc gcgcgttcac cgagccgccg cacctggaca actgcgtcga
     9481 gcagctcgcc caggcgccgc tcgacgtcat cgggtgcgcc ttcaccagct cggcctacaa
     9541 gcacgggccc cgcggcgagc acgcgctcgc cgcccggctg gccgagcacg cccgcggcat
     9601 gccggtcacc acgacctgcc tggccgccga gcaggccatc cgcgccctcg gcgtggaccg
     9661 gctcgccctg gtcaacccgc cgtggttcga cgaggagctc tcggcggctg gcgcggccta
     9721 cttcaccgcg ctcggcgtcg acgtggtcca ccacggggcg tgcggtctgc gcagcggcca
     9781 accgcacatc accccgcccg cgctctacga ctggatccgc tcggtcgcgg gcggcgcgtc
     9841 ggcggtgttc gtcgcgggca acggcatgcg cgcggtgggc atcatctcgg cgctggagga
     9901 ccggctcggc atcccggtgc tgaccgccaa ccaggtcctg ctctggcacg tcatggagct
     9961 caccggcacc acggtgtcgc ggcccgggta cggcaagctg atgaacatgt atccctctac
    10021 atagaccccg catcgggtat gctggcgggg acggagggct ctggtcgacg ctgaatcgtg
    10081 cctggttgcc cgtggtgccc gatgccactt gcgtggtcac cgggccgagg cggaggaggc
    10141 gcgacatcac cgccaagaag acagccccac cggccgcccc cgacctgtcc agcgtcgccg
    10201 agagctggac ccggctggtc gccctgcaca gccaggtcga ctcggtggtg ggccgggcgc
    10261 tgcaacgcca cttcagcatc ggcctctcgg agttcctcgc cctcatcgcc tgctccgccg
    10321 cccccgacgg cgagatgcgg atgcaggacc tcaccgacgc ggtcaacctc aaccagag
//
