LOCUS       NZ_JAMTCR010000002     10396 bp    DNA     linear   CON 29-AUG-2024
DEFINITION  Actinokineospora globicatena strain DSM 44256
            LV79DRAFT_scaffold00001.1, whole genome shotgun sequence.
ACCESSION   NZ_JAMTCR010000002
VERSION     NZ_JAMTCR010000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinokineospora globicatena
  ORGANISM  Actinokineospora globicatena
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Actinokineospora.
REFERENCE   1  (bases 1 to 3058629)
  AUTHORS   Goeker,M.
  TITLE     Genomic Encyclopedia of Archaeal and Bacterial Type Strains, Phase
            II (KMG-II): from individual species to whole genera
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3058629)
  AUTHORS   Goeker,M.
  TITLE     Genomic Encyclopedia of Archaeal and Bacterial Type Strains, Phase
            II (KMG-II): from individual species to whole genera
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3058629)
  AUTHORS   Goeker,M., Huntemann,M., Clum,A., Wang,J., Palaniappan,K.,
            Ritter,S., Chen,I.-M., Stamatis,D., Reddy,T., O'Malley,R., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2022) Prokaryote Super Program, DOE Joint Genome
            Institute, 1 Cyclotron Road, Mail Stop: 91R0183, Berkeley, CA 94720,
            USA
REFERENCE   4  (bases 1 to 3058629)
  AUTHORS   Goeker,M., Huntemann,M., Clum,A., Wang,J., Palaniappan,K.,
            Ritter,S., Chen,I.-M., Stamatis,D., Reddy,T., O'Malley,R., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2022) Prokaryote Super Program, DOE Joint Genome
            Institute, 1 Cyclotron Road, Mail Stop: 91R0183, Berkeley, CA 94720,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: AllPaths v. r46652
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 261.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024171945.1-RS_2024_08_29
            Annotation Date                   :: 08/29/2024 06:43:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,731
            CDSs (total)                      :: 6,666
            Genes (coding)                    :: 6,609
            CDSs (with protein)               :: 6,609
            Genes (RNA)                       :: 65
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 8 of 57
            Pseudo Genes (incomplete)         :: 49 of 57
            Pseudo Genes (internal stop)      :: 2 of 57
            Pseudo Genes (multiple problems)  :: 1 of 57
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:43:01
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 568832
            Orig. end                         :: 579228
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAMTCR010000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10396
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="3"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10396
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10396
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5396](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5396)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(885..3608)
                     /locus_tag="LV79_RS02685"
                     /old_locus_tag="LV79_000525"
     CDS             complement(885..3608)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF015649.5"
                     /locus_tag="LV79_RS02685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LV79_000525"
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_253831661.1"
                     /transl_table=11
                     /translation="MGENTLVPGGPGLRGLLAMLLLEPNQVVSVEHVMDVLWDHEPPAT
                     ARTIVQGYVSRLRRWLSTVDPTRETRIQTAGPGYRLVVDEARIDVALARELLGQSRDRP
                     ASERAELLGRAQALWRGPELSGIGGRVRAPELAELRLTVIEARIDADLELGRHDDVIGE
                     LAALVDTHPFREHLVGQLVLALYRCGRRAAALEIYQRFAHRASRELGLDPGPGLRELHS
                     RILRDDTTLLEPRPTPVITPRVGVLTPAQLPAAPPGFTGRDAELGWLDGIRDTADGTAP
                     AIAVLVGSAGVGKSALAVVWGTRSAAEFPDGRLYASLRGFDPRHEPTEPAEVLARFLLA
                     LGVAAEHIPDDLAERTALYHSLLAERRVLVLLDDARDSDQVRPLLPGAPGSMVLVTSRR
                     KLDGLVTHGAALRVLGTLPPEEAEAVIEHAAGPPADHERALRTRLARLCDHLPLALRIA
                     GARLVVSPQWSLRELVGELADERTRLGALRVHDVDTGVRAALDLTVGGLDPALAATLRL
                     LGAVPGPTFGPYLVATMAETSPDTARERLRGLAALFLVTESEPDVYGMHDLVRLYAREL
                     GADDRLPSVLRHYLVAADLCRRQLRAAADDIDYADTEPDVPRPRVRGREDALAWFEREW
                     PNLVAAAEAGAKAGLHTQVWQLARMASEYRRVRSRRDDWEHVLRLGLDAAKAAGDRRGE
                     VLMRLSRCVLLTRFDQEHETVADAERAVAIATELRDSKLMAMGFNTVASALYGQRRYNE
                     ALAGYRKALELSGRAGYRLGEANLLNNIAQVHRNLGAPAEAVTPQAQAVAIFREVGDLG
                     FVGLALANLAELEHELNLPEAAEVHAKEAISLAEDNGLDLTEAFAREVFARVLRDRGEI
                     AAARVELTLALELYRLVQSPLADGVQAGLAALPTDV"
     gene            complement(4057..4971)
                     /gene="thpD"
                     /locus_tag="LV79_RS02690"
                     /old_locus_tag="LV79_000527"
     CDS             complement(4057..4971)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806918.1"
                     /locus_tag="LV79_RS02690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LV79_000527"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_253831663.1"
                     /sec_met_domain="PhyH (E-value: 2.5e-33, bitscore: 113.4,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLTDTHLNTVSRADRYPTRTTEPLAPFARTEPTVWGGLTGPIDP
                     ATLAAHDSRGYSRYDGLLSPAEVAAFRAELDRLATDEAVRADERTIVERESAEVRSIFE
                     VHRSSELIAELVRDPRVLDRARQVLGSEVYVHQSRVNYMPGFTGKGFYWHSDFETWHAE
                     DGMPLPRAVSMSIALTDNHPFNGGLMVMPGSQRTFVPCVGETPADHYKASLTEQEIGVP
                     SQRAISLLAAEHGIDQFTGPAGSALLFDSNLMHGSGNNITPLPRSNIFVVFNSVENALV
                     DPYAAAAPRPGFIAARTVEPLVR"
     gene            complement(5001..5396)
                     /locus_tag="LV79_RS02695"
                     /old_locus_tag="LV79_000528"
     CDS             complement(5001..5396)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007025600.1"
                     /locus_tag="LV79_RS02695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LV79_000528"
                     /product="ectoine synthase"
                     /protein_id="WP_253831665.1"
                     /sec_met_domain="ectoine_synt (E-value: 4.8e-51, bitscore:
                     169.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRTLAEITDTDRDVTTEHWRSKRIVLAGDGVGFSVHETVLEPG
                     AVNDFWYANHIEAVFIVEGDGELLDKETGEVHQLAPGSIYVLDGNERHQLRPRTRMRTL
                     CVFNPPVTGREVHDENGVYPLVVEDKS"
     gene            complement(5433..6689)
                     /gene="ectB"
                     /locus_tag="LV79_RS02700"
                     /old_locus_tag="LV79_000529"
     CDS             complement(5433..6689)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (22-357). E-value: 3.1e-70.
                     Score: 228.8. Matches aSDomain:
                     nrpspksdomains_LV79_RS02700_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230319.1"
                     /locus_tag="LV79_RS02700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LV79_000529"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_253831667.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4.5e-73, bitscore:
                     243.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVFETLESEVRSYSRGWPVVFDRAVGSHLYDESGKSYVDFFAGA
                     GALNYGHNNPVLKQAVVDYIMRDGVTHALDMNTVAKRDFLESFRDVVLGPRELDYKVMF
                     PGPAGANAVEAALKLARKVTGRQSVVNFTNAFHGMTLGALSVTGNSMKRGGAGIPLVHA
                     TPMPFDNYLDGQVPDFLWFERLLADSGSGLNEPAAVIVETVQGEGGINAARVEWLRALA
                     DLCKRHGILLIVDDVQMGCGRTGPFFSFEVAGITPDIVCLSKSIGAFGLPLALTLIRPE
                     LDVWEPGEHNGTFRGISPAFVAATEALRTYWTDDTLEKGTLAKGEQVERVMHGIAEAHP
                     TAELKVKGRGLARGLRFADGETAGAVCRAAFDRGLLVETSGPDGEVAKVMPPLTITDDE
                     LDAGLGILTEAVAATLTRR"
     aSDomain        complement(5619..6623)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LV79_RS02700_Aminotran_3.1"
                     /evalue="3.10E-70"
                     /label="LV79_RS02700_Aminotran_3.1"
                     /locus_tag="LV79_RS02700"
                     /protein_end="357"
                     /protein_start="22"
                     /score="228.8"
                     /tool="antismash"
                     /translation="DRAVGSHLYDESGKSYVDFFAGAGALNYGHNNPVLKQAVVDYIMR
                     DGVTHALDMNTVAKRDFLESFRDVVLGPRELDYKVMFPGPAGANAVEAALKLARKVTGR
                     QSVVNFTNAFHGMTLGALSVTGNSMKRGGAGIPLVHATPMPFDNYLDGQVPDFLWFERL
                     LADSGSGLNEPAAVIVETVQGEGGINAARVEWLRALADLCKRHGILLIVDDVQMGCGRT
                     GPFFSFEVAGITPDIVCLSKSIGAFGLPLALTLIRPELDVWEPGEHNGTFRGISPAFVA
                     ATEALRTYWTDDTLEKGTLAKGEQVERVMHGIAEAHPTAELKVKGRGLARGLRF"
     gene            complement(6746..7264)
                     /gene="ectA"
                     /locus_tag="LV79_RS02705"
                     /old_locus_tag="LV79_000530"
     CDS             complement(6746..7264)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806913.1"
                     /locus_tag="LV79_RS02705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LV79_000530"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_253831669.1"
                     /transl_table=11
                     /translation="MSGETHDVVIAEPSTEDGSELWRIARDSRTLDLNSSYAYLLWCRD
                     FADTSAVATVDGRPVGFVIGFVRPSEPDTVVVWQIAVDSGQRGKGLAGRLLDHLLDRLA
                     PRGVRHLETTITADNAASIALFGALARRRGTRMRQADLFAADHFPDDHAAEDLYRIGPF
                     APAAVAVGV"
     gene            complement(7405..8448)
                     /gene="mgrA"
                     /locus_tag="LV79_RS02710"
                     /old_locus_tag="LV79_000531"
     CDS             complement(7405..8448)
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="mgrA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155093.1"
                     /locus_tag="LV79_RS02710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LV79_000531"
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="WP_253831671.1"
                     /transl_table=11
                     /translation="MPFPAAPYRAEESRYDRADYRRSGRSGLRLPPVSLGLWHNFGGDR
                     PLDTGRAILRRAFDLGVTHFDLANNYGPPYGSAEENFGRVLATDFKPYRDEIVVSTKAG
                     YDMWPGPYGEWGSRKYVLSSLDQSLTRLGLDYVDIFYSHRFDPDTPLEETIGALVTAVQ
                     QGKALYVGVSSYSPAATRQAAQILRAANVPLLIHQPSYSLLNRWIEPELLDTLDELGAG
                     CIAFSPLGQGLLTDRYLDGIPEGSRASRDSSLSPELISEENLARVRGLNQVARERGQTL
                     AQLSIAWVLRDRRVTSALIGASSVAQLEANLAAVDAPPFTDTELAEIDRYAVDSGIDLW
                     AQSREAG"
     gene            complement(8491..9102)
                     /locus_tag="LV79_RS02715"
                     /old_locus_tag="LV79_000532"
     CDS             complement(8491..9102)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LV79_RS02715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LV79_000532"
                     /product="hypothetical protein"
                     /protein_id="WP_253831673.1"
                     /transl_table=11
                     /translation="MSAAFDDMVRRASAGQPGALAVLRAELMAQPRAWLVDQLLATVAP
                     SSGYRPTQVELDEQRLVEHTARFGAWDTSACLVGPPPPAGQLIDRDQRTAHGEALLVEA
                     KDLLHTLLTGPTGLSPIRRARLTLTLPDQVVAVFGFLRGTITRTPVGGGQTALLVDYPE
                     VAADLVGDAVVAALRVVNGLELNEAVLYARRADVDASTLI"
     gene            complement(9163..9978)
                     /locus_tag="LV79_RS02720"
                     /old_locus_tag="LV79_000533"
     CDS             complement(9163..9978)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PIG-L"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983982.1"
                     /locus_tag="LV79_RS02720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LV79_000533"
                     /product="PIG-L family deacetylase"
                     /protein_id="WP_253831675.1"
                     /sec_met_domain="PIG-L (E-value: 5e-29, bitscore: 98.9,
                     seeds: 137, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATLVTFHAHPDDECINCGGVMRKAADEGHRVVLVVATRGELGEV
                     EDGFLAPGEDLTTRRVAETHAAAEILGVSRVEFLGYTDSGMMGEPSNDTPGTFWTASVD
                     EAAERLAKILVEESADVLTTYDDNGGYGHPDHIQVHRVGRRAAELAGTARVYQATANRD
                     DVRKHMGAAAAADAEAAAAQGIEAPESFGPPEEWEIGKPLEELTARVDVRAYVAAKRAA
                     MRAHASQISEESFFLSMPDPVFAEAFGVEWFIREGAGPGITETDIMAGL"
     misc_feature    9973..9983
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     misc_feature    10019..10034
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.78"
                     /tool="antismash"
     misc_feature    10328..10339
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
ORIGIN
        1 gacgacggtg ggtacgtggg gtgggccgac cggctcgccg agatgctggc cgcgcggcgg
       61 cccggtttgc ggtacgcgaa cctggcgctg cgcgggaaga tgctgcagga gatcatcgac
      121 gagcaggtgc cggtggcgct aaccgcgcgc cctgacctcg tcacgctctg cggtggtggc
      181 aacgacatca tcacgccggg caccgatgtc gacgacgtgg ccgcgctgtt cgagaccgcg
      241 gtggtcgcgc tgcgcgaggc gggctgcgac gtcgtggtgt tcaccggtcc ggatccgaag
      301 gtgcagccgc tgatccgccg gttccgcggc aaggtggcca tctacaacgg acacctgcac
      361 gcgatcgccg agcggcacgg cgcgcggttg gtcgacctgt ggtcgatggg ggtgctgcac
      421 gaccggcggt cctggtgcga cgaccggttg cacttctcca gcgaggggca ccgccggatc
      481 gtgttgcgca ccgcggaggt gctcgacttc gaggtgtcgc aggactggcg cgaaccgctg
      541 cccgcggagg cgcgcaagcc gtggctgcgg ctgcggcagg acgacctcgc gtggacgacg
      601 acgcacctga tcccgtgggt gcgcaggcag atcaccgggg agtccctcgg cgacggcctg
      661 caccccaagc gcccggagtt ggcgccggtc aagggggacg accgcacggc gggggtctga
      721 gtccacagcg ggcggacgac gaccggccgg ctctccctcc cccgagagcg ccggccggtg
      781 cgtcgggccg cacccccgtg gttcccgccc gcggccctgg gtgaccaccg gtcaccctgc
      841 gccgagaggg tgccggccca cccgcggcga cggctaagcg ttcgctaaac gtcggtgggc
      901 agcgccgcca gtccagcttg gacgccgtcg gcgagcggtg actgcacgag ccggtacagc
      961 tcgagtgcga gggtcagctc cactctggcc gccgcgatct cgccgcggtc gcgcagcacc
     1021 ctggcgaaca cctcgcgggc gaaggcctcg gtcaggtcga gcccgttgtc ctcggcgagc
     1081 gagatcgcct ccttggcgtg cacctcggcc gcctcgggca agttgagctc gtgctccagt
     1141 tcggccaggt tggccagggc cagcccgacg aacccgaggt cgccgacctc gcggaagatg
     1201 gcgaccgcct gcgcctgcgg ggtgaccgcc tcggcggggg cgccgaggtt gcggtgcacc
     1261 tgcgcgatgt tgttgagcag gttggcttcc ccgagccggt acccggcccg accggacagc
     1321 tccagtgcct tgcggtaacc ggccagtgcc tcgttgtagc ggcgctggcc gtagagagcg
     1381 ctcgcgaccg tgttgaagcc catcgccatc agcttggagt cgcgcagctc ggtcgcgatg
     1441 gccacggcgc gctcggcgtc ggcgaccgtc tcgtgctcct ggtcgaaccg ggtcagcagc
     1501 acgcaccggc tcagccgcat caggacctcg cccctgcggt cgcccgccgc cttcgccgcg
     1561 tccaacccga ggcgcagcac gtgttcccag tcatcgcggc gggagcggac cctgcggtac
     1621 tcgcttgcca tcctggccag ttgccagacc tgggtgtgca gccccgcttt ggcgcccgcc
     1681 tcggcggcgg cgaccaggtt gggccactcc cgctcgaacc aggcgagcgc gtcctcgcgg
     1741 ccgcgcaccc ggggccgggg gacgtccggt tcggtgtcgg cgtagtcgat gtcgtcggcg
     1801 gcggcgcgca actgcctgcg gcacaggtcg gcggcgacca ggtagtggcg cagcaccgac
     1861 ggcaaccggt cgtcggcgcc cagttccctg gcgtagagcc gaaccaggtc gtgcatgccg
     1921 tagacgtccg gttcggactc ggtgaccagg aacagcgcgg ccagcccgcg caaccgttcg
     1981 cgcgcggtgt ccggtgaggt ctcggccatg gtggccacca ggtacgggcc gaacgtcggt
     2041 ccggggaccg cgccgagcaa ccgcagcgtc gcggccagcg cggggtccag accaccgacg
     2101 gtcaggtcca gtgcggcgcg caccccggtg tcgacgtcgt gcacgcgcag cgcgccgagc
     2161 ctggtgcgct cgtcggccaa ctcaccgacc agttcccgaa gtgaccactg tggactgacg
     2221 accagccggg cacccgcgat ccgcagcgcg agcggcaagt ggtcgcacaa ccgggcgagc
     2281 ctcgtgcgca acgcccgctc gtggtcggct ggcggaccgg cggcgtgctc gatgaccgct
     2341 tccgcttcct ccggcgggag cgtgccgagc acccgcagcg cggcgccgtg cgtcaccaac
     2401 ccgtcgagct tgcgcctgct ggtgaccagc accatcgacc ctggcgcgcc gggcagcagc
     2461 gggcgcacct ggtcggagtc gcgggcgtcg tcgagcagca cgagcacccg gcgctcggcc
     2521 agcagcgagt ggtagagcgc ggtccgctcg gcgaggtcgt cggggatgtg ctccgcggcg
     2581 acgccgagcg cgagcaggaa ccgggccagc acctcggcgg gctcggtggg ctcgtgcctg
     2641 gggtcgaagc cgcgcaagga cgcgtacagc cgcccatcgg ggaactccgc ggcggagcgg
     2701 gttccccaca ccacggcgag cgcgctcttg ccgacaccgg ccgagccgac cagtacggcg
     2761 atcgcgggtg cggtgccgtc cgcggtgtcg cggatgccgt ccagccagcc gagttcggcg
     2821 tcccgaccgg tgaaccccgg tggggcggct gggagttgcg cgggggtcag cacgccgacg
     2881 cgcggtgtga tcaccggggt cgggcgtggt tcgagcaacg tggtgtcgtc gcgcaggatc
     2941 cggctgtgca gctcgcgcag accgggaccc gggtccagtc ccagttcccg ggacgcgcgg
     3001 tgggcgaagc gctggtagat ctccagcgcg gccgcccgtc taccgcagcg gtacagcgcg
     3061 aggacgagct gcccgaccag gtgttcgcgg aacgggtgcg tgtcgaccag cgcggccagt
     3121 tcgccgatga cgtcgtcgtg gcggcccagt tcgaggtcgg cgtcgatgcg cgcctcgatc
     3181 acggtcagcc gcagctcggc cagttccggc gcgcggacgc ggccgccgat gccggacagc
     3241 tcaggtccgc gccacagcgc ctgggccctg cccagcagtt cggcgcgctc gctcgccggt
     3301 cggtcccgcg actggccgag caactccctg gccagtgcga catcgatgcg ggcctcgtcg
     3361 accaccagcc ggtagccggg ccccgccgtc tggatcctgg tctcccgcgt gggatccaca
     3421 gtggacagcc aacggcgcag cctggacacg tagccctgca cgatggtgcg ggcggtcgca
     3481 ggcggctcgt gatcccacag cacatccatc acgtgctcga ccgagacgac ctggttgggc
     3541 tccagcagca gcatcgccag cagtccacgc agccccggcc ccccgggtac cagcgtgttc
     3601 tcccccacca gcgcgcacac cgggcccagt acgcaaaacc gcggtccgcc ttgctccgga
     3661 cgcgcgagcg cgacatgcgc cactcggtac caccccccga tcggaccgac tcccctaaag
     3721 ccggttccca gcgctcccgg cacaaaccta gggcgaaaca gcaggcgcgg gcgcggttgt
     3781 ggcactagtc aaccggatgg cctaaccgcg tgtgggggtg aagtgcgccg gtagcaccaa
     3841 aaacgggcgg gaaaggacca gcgaccgggt gcgggcatag gtcggccccg ccgccacacc
     3901 gatcccgaca tcggtgggca cggggccgta ctccgccgca gttggtccac agcacgtcgg
     3961 gttcaccccg agtgggaacc acgaccagct cttgatcaac cggtggcgga gcgtgcgctg
     4021 atggtaacag atggattacg catgatcacc ggcgggtcac cgcaccagcg gctcgaccgt
     4081 gcgcgcggcg atgaaaccgg gccgtggcgc ggccgccgcg tacgggtcga ccagcgcgtt
     4141 ctccaccgag ttgaacacga cgaagatgtt cgaccgcggc aacggcgtga tgttgttccc
     4201 cgagccgtgc atcaggttcg agtcgaacag cagcgccgac ccggccgggc cggtgaactg
     4261 gtcgatgccg tgctcggccg cgagcaggga gatcgcgcgc tggctcggga cgccgatctc
     4321 ctgctcggtc agcgaggcct tgtagtggtc ggccggggtc tcgccgacgc acggcacgaa
     4381 ggtgcgctgg gaaccgggca tcaccatcag cccgccgttg aacgggtggt tgtcggtgag
     4441 cgcgatggac atgctcaccg cgcgcggcag cggcatcccg tcctcggcgt gccaggtctc
     4501 gaagtcggag tgccagtaga agcccttgcc cgtgaacccg ggcatgtagt tgacccggct
     4561 ctggtgcacg tacacctcgg agccgagcac ctgccgcgcc cggtcgagca ccctcgggtc
     4621 gcgcacgagc tcggcgatca gctcgctgct gcggtgcacc tcgaagatcg agcgcacctc
     4681 ggcggactcg cgctcgacga tggtgcgctc gtcggcgcgc accgcctcgt cggtggccag
     4741 ccggtcgagc tcggcgcgga aggcggcgac ctcggccggg ctgagcaggc cgtcgtagcg
     4801 ggagtagccg cgggagtcgt gcgcggccaa cgtcgccggg tcgatcgggc cggtcagccc
     4861 gccccagacg gtcggctcgg tgcgggcgaa cggcgccagc ggctcggtgg tgcgggtcgg
     4921 gtagcggtcg gcgcgggaga cggtgttgag gtgggtgtcg gtgagtgtca tcagccctcc
     4981 tcggctggcc tgctcggggt tcacgacttg tcttcgacga cgagcgggta gaccccgttc
     5041 tcgtcgtgca cctcccggcc ggtgaccggg gggttgaaca cgcacagggt gcgcatgcgg
     5101 gtgcgcgggc gcagctggtg gcgctcgttg ccgtcgagca cgtagatgga ccccggcgcg
     5161 agctggtgga cctcgccggt ctccttgtcc agcagttcgc cgtcgccctc gacgatgaag
     5221 acggcctcga tgtggttggc gtaccagaag tcgttgaccg cgcccggctc cagcacggtc
     5281 tcgtggacgg agaatccgac gccgtcaccg gcgagcacga tgcgcttgct gcgccagtgc
     5341 tcggtcgtga cgtcgcggtc ggtgtcggtg atctcggcaa gcgtgcggac gatcatgggt
     5401 cctcctcgcg ctctggtggt cagcggcagt ggtcagcggc gggtcagggt ggcggcgacg
     5461 gcctcggtca ggatgcccag gcccgcgtcc agctcgtcgt cggtgatggt cagcggcggc
     5521 atgaccttgg cgacctcgcc gtcggggccg gaggtctcca ccagcaggcc gcggtcgaag
     5581 gcggcgcggc acaccgcacc ggcggtctcg ccgtcggcga agcgcaggcc cctggccaga
     5641 ccacgaccct tgaccttgag ctcggcggtc gggtgcgcct cggcgatgcc gtgcatgacc
     5701 cgctccacct gctccccctt ggccagcgtg cccttctcca gggtgtcgtc ggtccagtag
     5761 gtgcgcagcg cctcggtagc cgcgacgaac gccgggctga tgccgcggaa ggtgccgttg
     5821 tgctcgccgg gctcccagac gtcgagctcc gggcggatca gggtgagcgc gagcggcagg
     5881 ccgaaggcgc cgatggactt ggacaggcag acgatgtccg gggtgatgcc cgcgacctcg
     5941 aagctgaaga acggcccggt gcggccgcag cccatctgca cgtcgtcgac gatgagcagg
     6001 atgccgtgcc gcttgcacag gtcggcgagc gcccgcagcc actccacccg cgcggcgttg
     6061 atcccgccct cgccctgcac ggtctcgacg atcaccgcgg cgggctcgtt gaggccgctg
     6121 ccgctgtcgg cgagcagccg ctcgaaccac aggaagtccg gcacctggcc gtcgaggtag
     6181 ttgtcgaacg gcatcggggt ggcgtgcacc agcgggatgc ccgcgccgcc gcgcttcatc
     6241 gagttgccgg tgaccgacag cgcgccgagg gtcatgccgt ggaaggcgtt ggtgaagttg
     6301 accaccgact ggcggccggt gaccttgcgg gccagcttca gcgccgcctc gaccgcgttc
     6361 gcgcccgcgg ggccggggaa catcaccttg tagtccagct cgcgcggacc gagcacgacg
     6421 tcgcggaagg actccaggaa gtcgcgcttg gccacggtgt tcatgtccag cgcgtgggtc
     6481 acgccgtcgc gcatgatgta gtcgacgacg gcctgtttga gcaccgggtt gttgtggccg
     6541 tagttcaacg cgcccgcgcc cgcaaagaag tccacatagg acttgccaga ctcgtcgtag
     6601 aggtggctgc cgaccgcgcg gtcgaacacg accggccacc cgcggctgta gctgcggacc
     6661 tcggactcga gggtttcgaa cacactcatc gtgaaatctc cctccccagc gggaaagtag
     6721 ggcgaacggg ccagtgggac gcgggtcaga ccccgacggc gacggcagcc ggggcgaacg
     6781 gaccgatgcg gtacaggtcc tccgcggcgt ggtcgtcggg gaagtggtcg gcggcgaaca
     6841 ggtcggcctg gcgcatccgc gtcccccgcc gccgggccag tgcgccgaac aacgcgatgg
     6901 acgcggcgtt gtcagcggtg atggtggtct ccaggtgccg cacaccgcgc ggcgcgagcc
     6961 ggtcgagcag gtggtcgagc agcctgcccg cgaggccctt gccgcgctgg ccgctgtcga
     7021 cggcgatctg ccacaccaca acggtgtccg gctcactcgg ccggacgaaa ccgatcacga
     7081 agccgaccgg tcggccgtcg acggtggcga cggcggaggt gtcggcgaag tcgcggcacc
     7141 agagcaggta ggcgtaggag gagttcaggt cgagcgtgcg cgagtcgcgg gcgatccgcc
     7201 acagctcgga gccgtcctct gtggacggct cggcgatcac cacgtcgtgc gtttccccgg
     7261 acatacccag ggaacgtaac agcgatgttt cgacggttgt ggacggggag aaccagcacc
     7321 ccgcccgtca ccttgggttt cggaggatca ccggtgagga ggacaacacc ggcgttgtcc
     7381 tccgcaccgc gaccgtgtta aggatcagcc cgcctcgcgc gactgcgccc acaggtcgat
     7441 cccggaatcc accgcgtacc tgtcgatctc ggccagttcg gtgtcggtga acgggggcgc
     7501 gtcgacggcg gccaggttgg cctccagctg ggcgaccgag ctggcgccga tgagcgccga
     7561 ggtgacccgc cggtcgcgca gcacccacgc gatcgagagc tgggccaacg tctggccgcg
     7621 ctcgcgggcg acctggttga gcccgcgcac cctggccagg ttctcctcgg agatcagctc
     7681 gggcgacagc gacgagtcgc ggctggcccg ggacccctcg gggatgccgt cgaggtaccg
     7741 gtcggtgagc aggccctgcc cgagcgggga gaacgcgatg cagcccgcgc ccagctcgtc
     7801 gagcgtgtcg agcagctcgg gctcgatcca ccggttgagc agcgagtacg acggctggtg
     7861 gatcagcagc gggacgttgg cggcgcgcag gatctgcgcg gcctgcctgg tcgccgccgg
     7921 ggagtacgac gacaccccga cgtagagcgc cttgccctgc tgcaccgccg tgaccagcgc
     7981 gccgatggtc tcctccagcg gcgtgtccgg gtcgaagcgg tgcgagtaga agatgtcgac
     8041 gtagtcgagg ccgagcctgg tcagcgactg gtccaaagag gacagcacat acttgcggga
     8101 accccactcc ccgtacggac ccggccacat gtcgtagccc gccttggtgg agaccacgat
     8161 ctcgtcgcgg tagggcttga agtcggtggc cagcacgcgg ccgaagttct cctccgcgct
     8221 gccgtagggc gggccgtagt tgttggccag gtcgaagtgg gtgacaccga ggtcgaacgc
     8281 gcggcgcagg atggcgcggc cggtgtcgag cggtcggtcg ccgccgaagt tgtgccacag
     8341 gccgagcgag accggcggca gccgcaggcc actgcgcccg gacctgcggt agtcggcgcg
     8401 gtcgtagcgg ctctcctcgg cgcggtaggg cgcggctggg aacggcatgg gactcctcgg
     8461 gtcgcggggg cgctaccggg tgattctcgc tcagatcagc gtgctggcgt ccacgtcggc
     8521 gcgccgcgcg tagagcacgg cctcgttgag ctccagcccg ttgaccaccc gcagcgcggc
     8581 caccacggcg tccccgacca ggtcggcggc cacttcgggg tagtcgacga gcagcgcggt
     8641 ctggccgccg ccgaccgggg tcctggtgat cgtgccgcgc aagaagccga acacggcgac
     8701 gacctggtcg ggcagcgtca gggtgagccg ggcgcggcgg atcggggaca gcccggtcgg
     8761 cccggtcagc agcgtgtgca gcaggtcctt ggcctcgacg agcagcgcct cgccgtgcgc
     8821 ggtgcgctgg tcgcggtcga tcagctgccc ggccggtggc ggcggcccga ccaggcaggc
     8881 gctggtgtcc caggcgccga accgggcggt gtgctcgacc agccgctgct cgtcgagctc
     8941 gacctgggtg ggccggtacc cgctggacgg ggccacggtg gccagcagct ggtcgaccag
     9001 ccaggcccgc ggctgggcca tcagctcggc gcgcagcacc gcgagcgcac ccggctgccc
     9061 cgccgaggcc cggcggacca tgtcgtcgaa ggctgcggac acgctcggca aggtaagccg
     9121 ccccggcggg ccagtaccag cctcgtcacc cgatggtggg cctcagagcc cggccatgat
     9181 gtcggtctcg gtgatccccg ggcctgcccc ctcgcggatg aaccactcca cgccgaacgc
     9241 ctcggcgaag accgggtccg gcatggacag gaagaagctc tcctcggaga tctggctggc
     9301 gtgcgcgcgc atcgcggccc gcttggccgc cacatacgcc cgcacgtcca cgcgggcggt
     9361 cagctcctcc agcggcttgc cgatctccca ctcctccggc gggccgaacg actcgggcgc
     9421 ctcgatgccc tgcgcggcgg ctgcctcggc gtcagcggcc gcggcggcgc ccatgtgctt
     9481 gcggacgtcg tcgcggttcg cggtcgcctg gtacacccgg gcggtcccgg ccagctcggc
     9541 ggcgcggcgg ccgacccggt gcacctggat gtggtcgggg tggccgtagc cgccgttgtc
     9601 gtcgtaggtg gtcagcacgt cggccgactc ctcgacgagg atcttggcca ggcgctcggc
     9661 cgcctcgtcg accgacgcgg tccagaaggt gcccggggtg tcgttggacg gctcgcccat
     9721 catcccggag tcggtgtagc cgaggaactc cacacgggac acaccgagga tctcggcggc
     9781 cgcgtgcgtc tcggcgaccc ggcgggtggt caggtcctcg ccgggggcca ggaacccgtc
     9841 ctcgacctcg ccgagctcgc ccctggtcgc caccacgagg acgacgcggt ggccctcgtc
     9901 ggcggctttg cgcatgacgc caccgcagtt gatgcactcg tcgtcggggt gggcgtggaa
     9961 ggtcaccagt gttgccatgg caccaaagta cccaccccgg ccgacagacc ggcggtcgtg
    10021 gaccgccggg tcagctgacc gcgacgcgct ccggcacggc gacctgctcg accggggtca
    10081 cctcggggct gccgttgacg atcgagtcca ggatctcgcc gacccggacg gccgtggtcg
    10141 acaggagtgt cgcggtgaga ccgtggctgt gctcggcgcc ctggaggtaa acaccagccg
    10201 tcacgttggc agcggtgctg acccggtagt cccgcccgac gatcggtagg cctgcctcgt
    10261 cgcgctcgca cacagcggca gcgcggccga ggaggtcgaa caggtcaccc tgacggaagc
    10321 cggtggcgta aacgaccgcg tcgcactcaa gaacctcggt ctcgccggtc ggtaagaact
    10381 ccacggtcgc gtagac
//
