LOCUS       NZ_JAMYDW010000007     20747 bp    DNA     linear   CON 24-SEP-2024
DEFINITION  Bacillus subtilis strain X0416 contig7, whole genome shotgun
            sequence.
ACCESSION   NZ_JAMYDW010000007
VERSION     NZ_JAMYDW010000007.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 246298)
  AUTHORS   Xue,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Food Academy, Sichuan Agricultural
            University, Xinkang Road No. 48, Ya'an, Sichuan 625000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.0.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024128815.1-RS_2024_09_24
            Annotation Date                   :: 09/24/2024 05:23:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,267
            CDSs (total)                      :: 4,141
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 126
            rRNAs                             :: 12, 14, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 9 (5S)
            partial rRNAs                     :: 3, 14, 10 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 75 of 141
            Pseudo Genes (incomplete)         :: 74 of 141
            Pseudo Genes (internal stop)      :: 17 of 141
            Pseudo Genes (multiple problems)  :: 21 of 141
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:26:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 48780
            Orig. end                         :: 69527
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAMYDW010000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20747
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="CDPS"
                     /region_number="1"
                     /rules="CDPS"
                     /tool="antismash"
     cand_cluster    1..20747
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="CDPS"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20747
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="False"
                     /core_location="[10000:10747](+)"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10747
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     gene            1023..3896
                     /gene="uvrA"
                     /locus_tag="NHM07_RS15460"
                     /old_locus_tag="NHM07_15460"
     CDS             1023..3896
                     /EC_number="3.1.25.-"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_process="GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="uvrA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391396.1"
                     /locus_tag="NHM07_RS15460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NHM07_15460"
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="WP_253137213.1"
                     /transl_table=11
                     /translation="MAMDRIEVKGARAHNLKNIDVTIPRDQLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMDKPDVDAIEGLSPAISIDQKTTSRNPRSTVGTVT
                     EIYDYLRLLYARVGKPHCPEHGIEITSQTIEQMVDRILEYPERTKLQVLAPIVSGRKGA
                     HVKVLEQIRKQGYVRVRIDGEMAELSDDIELEKNKKHSIEVVIDRIVVKEGVAARLSDS
                     LETALRLGEGRVMIDVIGEEELMFSEHHACPHCGFSIGELEPRLFSFNSPFGACPTCDG
                     LGMKLEVDADLVIPNQDLSLKENAVVPWTPISSQYYPQLLEAVCTHYGIDMDVPVKDLP
                     KHQLDKVLYGSGDDLIYFRYENDFGQIREGEIQFEGVLRNIERRYKETGSDFIREQMEQ
                     YMSQKSCPTCKGYRLKKEALAVLIDGRHIGKITELSVADALAFFKDLTLSEKDMQIANL
                     ILREIVERLSFLDKVGLDYLTLSRAAGTLSGGEAQRIRLATQIGSRLSGVLYILDEPSI
                     GLHQRDNDRLISALKNMRDLGNTLIVVEHDEDTMMAADYLIDIGPGAGIHGGQVISAGT
                     PEEVMEDPNSLTGSYLSGKKFIPLPPERRKPDGRYIEIKGASENNLKKVNAKFPLGTFT
                     AVTGVSGSGKSTLVNEILHKALAQKLHKAKAKPGSHKEIKGLDHLDKVIDIDQAPIGRT
                     PRSNPATYTGVFDDIRDVFAQTNEAKVRGYKKGRFSFNVKGGRCEACRGDGIIKIEMHF
                     LPDVYVPCEVCHGKRYNRETLEVTYKGKSISDVLDMTVEDALSFFENIPKIKRKLQTLY
                     DVGLGYITLGQPATTLSGGEAQRVKLASELHKRSTGRTLYILDEPTTGLHVDDIARLLV
                     VLQRLVDNGDTVLVIEHNLDIIKTADYIVDLGPEGGAGGGTIVASGTPEEITEVEESYT
                     GRYLKPVIERDKTRMKSLLKAKETATS"
     gene            4234..5148
                     /gene="hag"
                     /locus_tag="NHM07_RS15465"
                     /old_locus_tag="NHM07_15465"
     CDS             4234..5148
                     /codon_start=1
                     /gene="hag"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391416.1"
                     /locus_tag="NHM07_RS15465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NHM07_15465"
                     /product="flagellin Hag"
                     /protein_id="WP_046160896.1"
                     /transl_table=11
                     /translation="MRINHNIAALNTLNRLSSNNSASSKNMEKLSSGLRINRAGDDAAG
                     LAISEKMRGQIRGLEMASKNSQDGISLIQTAEGALTETHSILQRVRELVVQAGNTGTQD
                     GTDLQAIQDEISSLVEEIGGGTNANGISDRTEFNGKKLLNGAFSEDSGKSLTFQIGANG
                     SQQLSVNIESMAATAIGALDDDGNLVADQTVAKIDVTKFADNAADTATVGFDAQLKTVD
                     DAINTVSKQRSKLGAVQNRLEHTINNLSASGENLTAAESRIRDVDMAKEMSEFTKNNIL
                     SQASQAMLAQANQQPQNVLQLLR"
     gene            complement(5347..5577)
                     /locus_tag="NHM07_RS15470"
                     /old_locus_tag="NHM07_15470"
     CDS             complement(5347..5577)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391394.1"
                     /locus_tag="NHM07_RS15470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NHM07_15470"
                     /product="hypothetical protein"
                     /protein_id="WP_101501509.1"
                     /transl_table=11
                     /translation="MEKDPSDYTVTQESVLKLIHEQKRINREMIAELEQIHGPFPISHD
                     IQYIKVLLDSSNTHIVQDLMSVSKQLYKKTL"
     misc_feature    5546..5561
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.68"
                     /tool="antismash"
     gene            5795..6121
                     /gene="yvlA"
                     /locus_tag="NHM07_RS15475"
                     /old_locus_tag="NHM07_15475"
     CDS             5795..6121
                     /codon_start=1
                     /gene="yvlA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391393.1"
                     /locus_tag="NHM07_RS15475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NHM07_15475"
                     /product="protein YvlA"
                     /protein_id="WP_032726939.1"
                     /transl_table=11
                     /translation="MNRNQAIIASLCYFSVFIAPIIVPIVAYFVVNEKETKRHAIRSLI
                     SHIVPFVGWLFLFIALLGGAVAIDGDSLLPVFVIIGGAVIYFLVVIGIIIWNVIQGIKV
                     LREA"
     gene            6146..7243
                     /gene="yvlB"
                     /locus_tag="NHM07_RS15480"
                     /old_locus_tag="NHM07_15480"
     CDS             6146..7243
                     /codon_start=1
                     /gene="yvlB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391392.1"
                     /locus_tag="NHM07_RS15480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NHM07_15480"
                     /product="DUF4097 domain-containing protein"
                     /protein_id="WP_014478112.1"
                     /transl_table=11
                     /translation="MKQEKERILKLVEEGKLTAQEALTLIEKLDSDYKEKEEKITALSV
                     HVHDEEEPFTTAKKESGKPSLGAKLFDWIDSAVKKVKEVDLDLNFGHAYDVQHIFQFKD
                     TDFSSVELQIANGSVNIVPWEDDDIRAECQAKVYRADSQDAARHAFLQHIECEIKGNKF
                     FIRTEKKTMKTNVTLYIPQKEYDKIRVKLFNGPVRGEHLHVKEFSAKTTNGVLSFSHLT
                     AEKAIAETANGQIKLASHSCGTIEAETINGLIDLRGKSESIDVQSFNGNIAINVTESDC
                     RSIYAKTTTGSVELAIPDDLAVKAELKSNLGSLSHELMDVEMLKEKNDTIQKEMMFTSN
                     QAHDQNITVFSESLTGAIKLKYSQR"
     gene            7248..7445
                     /gene="yvlC"
                     /locus_tag="NHM07_RS15485"
                     /old_locus_tag="NHM07_15485"
     CDS             7248..7445
                     /codon_start=1
                     /gene="yvlC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391391.1"
                     /locus_tag="NHM07_RS15485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NHM07_15485"
                     /product="PspC domain-containing protein"
                     /protein_id="WP_003228065.1"
                     /transl_table=11
                     /translation="MNKLYRSEKNKKIAGVIGGLAEYFNWDASLLRVITVILAIMTSVL
                     PVLLIYIIWIFIVPSERDMK"
     gene            7447..7806
                     /gene="yvlD"
                     /locus_tag="NHM07_RS15490"
                     /old_locus_tag="NHM07_15490"
     CDS             7447..7806
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene="yvlD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391390.1"
                     /locus_tag="NHM07_RS15490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NHM07_15490"
                     /product="phage holin family protein"
                     /protein_id="WP_003228066.1"
                     /transl_table=11
                     /translation="MVKWAVSILVNALLLIVIDGYIDSIHISSIGAAIIASLILSILNV
                     LIKPLLIIFTLPVTMVTLGLFLFVINAITLMMTASIMGDSFQIDGFGTAIWASVILSVF
                     HLLIQKGILEPLRKK"
     gene            complement(7834..9045)
                     /gene="pchE"
                     /locus_tag="NHM07_RS15495"
                     /old_locus_tag="NHM07_15495"
     CDS             complement(7834..9045)
                     /codon_start=1
                     /gene="pchE"
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391389.1"
                     /locus_tag="NHM07_RS15495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NHM07_15495"
                     /product="MFS transporter"
                     /protein_id="WP_003228068.1"
                     /transl_table=11
                     /translation="MAHTKSKAVLILYTVCFSAFFASLSQNIYSPILPIIKESFHVSTA
                     MVNLSVSVFMIVTAIMQIILGAIIDFKGARIVLITGILATAAASIGCAVTTDFTLFLIF
                     RMIQAAGSAALPLIAATTIGQLFTGNERGSAMGTYQMLLSVAPAIAPVLGGFIGGAAGY
                     EGIFWILAAISIVLLVTNSITFPKDSPTESMQQAKGNVFAHYKSIFTNRTGNVILTLSF
                     VLFFIYFAVIVYLPILLTEHYHIDVGIAGLLYLPLALSTIAGTFLFKRIQAKIGLHTLF
                     IGSNVIAACSIILFAVTHSVSLVLMALTLALFGISMGVIPPLYSTMITNEFEHNRGSAI
                     GMFNFIRYTGMAAGPMVSAYLLTMMPSAMSFSLLGLGFAALSFCLLPPMFSPQKRTKQK
                     KHHM"
     gene            complement(9066..9575)
                     /gene="pchR"
                     /locus_tag="NHM07_RS15500"
                     /old_locus_tag="NHM07_15500"
     CDS             complement(9066..9575)
                     /codon_start=1
                     /gene="pchR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391388.1"
                     /locus_tag="NHM07_RS15500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NHM07_15500"
                     /product="pulcherriminic acid biosynthesis transcriptional
                     regulator PchR"
                     /protein_id="WP_003228073.1"
                     /transl_table=11
                     /translation="MSDLTKQMIYDIYVRLLHLNEQKANTSLQQFFKEAAEEDVAEIPK
                     NMTSIHVIDCIGQHEPINNAGIARKMNLSKANVTKISTKLIKEEFINSYQLTDNKKEVY
                     FKLTRKGRRIFDLHEKLHKKKELAFYQFLDSFSQEEQKAVLKFLEQLTSTLEAEQTDGT
                     PDKPVK"
     misc_feature    9857..9872
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 22.77"
                     /tool="antismash"
     misc_feature    complement(9859..9873)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.98"
                     /tool="antismash"
     misc_feature    complement(9860..9875)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 21.5"
                     /tool="antismash"
     gene            10001..10747
                     /gene="pchC"
                     /locus_tag="NHM07_RS15505"
                     /old_locus_tag="NHM07_15505"
     CDS             10001..10747
                     /EC_number="2.3.2.22"
                     /codon_start=1
                     /gene="pchC"
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391387.1"
                     /locus_tag="NHM07_RS15505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NHM07_15505"
                     /product="cyclo(L-leucyl-L-leucyl) synthase"
                     /protein_id="WP_253137216.1"
                     /sec_met_domain="CDPS (E-value: 5e-84, bitscore: 275.2,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGMVTERRSVHFIAEPLTENCREIFERRRHVLVGISPFNSWFSE
                     DYIYRLIGWAKAQFKSVSVLLAGHEAANLLEALGTPRGKAERKVRKEVSRNRRFAERAL
                     VAHGGDPKAIHTFSDFMDNKAYQLLRQEVEHAFFEQPHFRHACLDMSREAIIGRARGVS
                     LMMEEVSEDMLNLAVEYVIAELPFFIGAPDILEVEETLLAYHRPWKLGEKISNHEFSIC
                     MRPNQGYLIVQEMAQMLSEKRITCEG"
     gene            10763..11980
                     /gene="cypX"
                     /locus_tag="NHM07_RS15510"
                     /old_locus_tag="NHM07_15510"
     CDS             10763..11980
                     /EC_number="1.14.15.13"
                     /codon_start=1
                     /gene="cypX"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391386.1"
                     /locus_tag="NHM07_RS15510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NHM07_15510"
                     /product="pulcherriminic acid synthase"
                     /protein_id="WP_021480698.1"
                     /sec_met_domain="p450 (E-value: 1.7e-37, bitscore: 123.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQSIKLFSVLSDQFQNNPYAYFSQLREEDPVHYEESIDSYFISR
                     YHDVRYILQHPDIFTTKSLVERAEPVMRGPVLAQMHGKEHSAKRRIVVRSFIGDALDHL
                     SPLIKQNAENLLAPYLERGKIDLVNDFGKTFAVCVTMDMLGLDKRDHEKISEWHSGVAD
                     FITSISQSPEARAHSLWCSEQLSQYLMPVIEERRVNPGSDLISILCTSEYEGMAMSDKD
                     ILALILNVLLAATEPADKTLALMIYHLLNNPEQMNDVLADRSLVPRAIAETLRYKPPVQ
                     LIPRQLSQDTVVGGMEIKKDTIVFCMIGAANRDPEAFEQPDVFNIHREDLGIKSAFSGA
                     ARHLAFGSGIHNCVGAAFAKTEIEIVANIVLDKMRNIRLEEDFCYAESGLYTRGPVSLH
                     IVFDRA"
     gene            complement(12019..12479)
                     /gene="pchA"
                     /locus_tag="NHM07_RS15515"
                     /old_locus_tag="NHM07_15515"
                     /pseudo=""
     CDS             complement(12019..12479)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="pchA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391385.1"
                     /locus_tag="NHM07_RS15515"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="NHM07_15515"
                     /product="MarR family transcriptional regulator"
                     /pseudo=""
                     /transl_table=11
                     /translation="MNNSIHDELFQAIQQFALKRDKRVWQKVQIPSSQHLDHLKKTGH"
     gene            12644..16513
                     /gene="yvnB"
                     /locus_tag="NHM07_RS15520"
                     /old_locus_tag="NHM07_15520"
     CDS             12644..16513
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="yvnB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391384.1"
                     /locus_tag="NHM07_RS15520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NHM07_15520"
                     /product="metallophosphoesterase"
                     /protein_id="WP_253137217.1"
                     /transl_table=11
                     /translation="MRKYTVIVSILLSFLSVLSGGHHESKAFPVVQQELFQTPHYIPLL
                     ENPPQIDVKAEMRNNLMIEAQVRDNSYQAFSAFLFYKQSNELGYKMVPMDPTPGAVRKF
                     LAQIPKRLIWNSELEYYIVMSNGKQRVESETKKLKLEGYQADLAHIPELLITELAVDTK
                     NIGRADGYEFIEIFNTTDRTIDFKDYHIRYRYPKEGPDSDLIWRPDERILIPSGETFVV
                     WLKPAGHPELTSEDFNRYYQTQLKEGKNLAVIDETEGMANTRPRAVVISTNTGKDISVA
                     HYRKHALRRLSSVLYKYPLNGTAELLNISIGEKNPSPGTVLQAQVPEQKRKIKLDKEKP
                     VIEDLTDRKPVRPAESIELRADIRDRSLVKTAAFYYRTDENKPFKRILAEKDRNDNLYH
                     YIVYSPELIGKDQLEYYVAAGDGINEARTPVKMIDIKQTSKSHGLRMNIENRDTLSGTQ
                     FLKATTEGRADRIKLWIDGKKQVTEPAMEKEVYFAFDTRKTNLYFKNAVTMEGKVLKVF
                     DDTTNKYRTYSVPLPETLLRKGKQLQRITIRSGSKVSPFDTAENRDDFLVKNARLVLSD
                     GTVIRDQRVSPEKELFIGDNQRSNKSWQFQFNLPDGLFTSQLLEWDTSKLSEGAHHIQA
                     SDGKENVSLVVRVDNSGPHIEPNITEGQTYKGNLILQADMYDKWSKIEEAEASLDGESI
                     TLPYHTSSSDLLPGKHSLKVTATDLAGNKTVIERIFKTEREHPDQPEVIDSEADTHKAK
                     LSVRVKDPTNDAMDVGFYRGFQYTARNHVKIFKHASLTEPPKSFVPESETPFTNKELER
                     VSAADGKTVSTENKELFPYHRFEVTVDPSIDENDLAEAVWKGSSLPGRKVTMYAWNYRT
                     NEWQPVDSFVAKDDKPFTLKASVIAADFVRESKMNVIVQDEIPPAKDMYTFVWMSDTQY
                     YAESYPHIFDKQTEWIKDNQKQLNIKYVFHTGDIVDDSADIRQWKNADRSMSVLDKSGI
                     PYGVLAGNHDVGHKDGSYRAFGKYFGSDRFDKKLHYGGSYKNNRGHYDLISSNGNDYIM
                     LYMGWGITDEDIAWMNQVLKKHPDRMAILAFHEYLLVSGNRSPIGEKIFKEIVKPNPNV
                     VMVLSGHYHSAMRKTDELDDDGDGKPDRLVHQMLADYQGGPEGGQGYLRLLQFDQANDM
                     VHVSTYSPYVKDKNYYDTDTYGNKDEFSLSLDLKPRIKKVETDYFECNVYTNEELGKRE
                     QVKSGDTAEFRWDHLEPQSVYYWYIIVEDSFNGKTKSPIWKFKTKKETYRPAPDQFDFR
                     HVSNP"
     gene            complement(16533..17264)
                     /gene="nagR"
                     /gene_synonym="yvoA"
                     /locus_tag="NHM07_RS15525"
                     /old_locus_tag="NHM07_15525"
     CDS             complement(16533..17264)
                     /codon_start=1
                     /gene="nagR"
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /gene_synonym="yvoA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391383.1"
                     /locus_tag="NHM07_RS15525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NHM07_15525"
                     /product="N-acetylglucosamine utilization transcriptional
                     regulator NagR"
                     /protein_id="WP_253137220.1"
                     /transl_table=11
                     /translation="MNINKQSPIPIYYQIMEQLKTQIKSGELQPDMPLPSEREYAEQFG
                     ISRMTVRQALSNLVNEGLLYRLKGRGTFVSKPKMEQALQGLTSFTEDMKSRGMTPGSRL
                     IDYQLIDSTEELAAILGCGHPSSIHKITRVRLANDIPMAIESSHIPFELAGELNESHFQ
                     SSIYDHIERYNSIPISRAKQELEPSAATTEEANILGIQKGAPVLLIKRTTYLQNGTAFE
                     HAKSVYSGDRYTFVHYMDRLS"
     gene            complement(17282..18010)
                     /gene="nagB"
                     /locus_tag="NHM07_RS15530"
                     /old_locus_tag="NHM07_15530"
                     /pseudo=""
     CDS             complement(17282..18010)
                     /GO_function="GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA]"
                     /GO_process="GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="nagB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391382.1"
                     /locus_tag="NHM07_RS15530"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="NHM07_15530"
                     /product="glucosamine-6-phosphate deaminase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKVMECQTYEELSQKAARITADTIKEKPDAVLGLATGGTP"
     gene            complement(18007..19197)
                     /gene="nagA"
                     /locus_tag="NHM07_RS15535"
                     /old_locus_tag="NHM07_15535"
     CDS             complement(18007..19197)
                     /EC_number="3.5.1.25"
                     /GO_function="GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA]"
                     /GO_process="GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="nagA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391381.1"
                     /locus_tag="NHM07_RS15535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NHM07_15535"
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="WP_021480694.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 2.5e-43, bitscore:
                     142.4, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAESLLIKDIAIVTENEVIKNGYVGINDGKISTVSTERPKESYSK
                     EIQAPANSVLLPGMIDIHIHGGYGADTMDASFSALDIMSSRLPEEGTTSFLATTITQEH
                     GNISQALVNAREWKAAEESSLLGAELLGIHLEGPFVSPKRAGAQPKEWIRPSDVALFKK
                     WQQEAGGLIKIVTLAPEEDQHFELIRHLKEESIIASMGHTDADSAMLSDAAKAGASHMT
                     HLYNAMSPFHHREPGVIGTALAHDGFVTELIADGIHSHPLAAKLAFLAKGSSKLILITD
                     SMRAKGLKDGVYEFGGQSVTVRGRTALLSDGTLAGSILKMNEGARHMREFTNCSWTDIA
                     NITSANAAKQLGIFDRKGSVTVGKDADLVIVSSDCEVILTICRGNIAFISKEADQI"
     misc_feature    complement(19252..19267)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.26"
                     /tool="antismash"
     gene            19379..20311
                     /gene="hprK"
                     /locus_tag="NHM07_RS15540"
                     /old_locus_tag="NHM07_15540"
     CDS             19379..20311
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hprK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391380.1"
                     /locus_tag="NHM07_RS15540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NHM07_15540"
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="WP_003228097.1"
                     /transl_table=11
                     /translation="MAKVRTKDVMEQFNLELISGEEGINRPITMSDLSRPGIEIAGYFT
                     YYPRERVQLLGKTELSFFEQLPEEEKKQRMDSLCTDVTPAIILSRDMPIPQELIDASEK
                     NGVPVLRSPLKTTRLSSRLTNFLESRLAPTTAIHGVLVDIYGVGVLITGKSGVGKSETA
                     LELVKRGHRLVADDCVEIRQEDQDTLVGNAPELIEHLLEIRGLGIINVMTLFGAGAVRS
                     NKRITIVMNLELWEQGKQYDRLGLEEETMKIIDTEITKLTIPVRPGRNLAVIIEVAAMN
                     FRLKRMGLNAAEQFTNKLADVIEDGEQEE"
ORIGIN
        1 gcttcttgat tatttcccag acgattttat gatcgtggtg gatgagtcgc atgtgacaat
       61 tccccaggtg cgcggcatgt ttaacggaga ccaggcgcgg aaacaggtgc tcgtggatca
      121 cggcttccgg cttccatcag cgcttgataa ccgtccgctt cgttttgagg aatttgaaaa
      181 gcatatgcac aatatcgtgt atgtatccgc aacaccaggg ccgtacgaga ttgaacatac
      241 agatgaaatg gtcgaacaga tcattcgtcc gacggggctt cttgacccgc ttattgacgt
      301 ccgtccaatc gaaggtcaga ttgacgactt gatcggcgaa attcaagcta gaatagagcg
      361 gaatgaacgg gtgcttgtga caactttgac gaagaagatg tcagaggatc tgaccgatta
      421 tttaaaggaa atcggcatta aagtaaacta tctgcattct gaaatcaaga cgcttgaacg
      481 gattgaaatt atccgcgacc tgcgccttgg caagtacgat gtgcttgtcg gcatcaacct
      541 gctgagggaa ggtttggaca ttcccgaagt atcccttgtt gcgattttgg atgcggataa
      601 ggaaggtttc cttcgttcgg agcggtcgct gatccagacc atcggacggg cagcgagaaa
      661 tgcggaaggc cgcgtcatta tgtacgccga taaaataacg aagtctatgg aaattgcgat
      721 taatgaaaca aaacgccgcc gcgagcagca ggagcggttt aatgaagagc acggcattac
      781 gccgaaaacg ataaataaag aaattcgcga tgtcattcgt gccacagtag cagctgagga
      841 taaagcggaa tacaaaacaa aagccgcgcc gaagctgtcg aaaatgacga agaaagaacg
      901 ccagaaagtc gttgaacaga tggagcatga aatgaaggaa gccgccaagg cgcttgactt
      961 tgaaagagcc gcggagcttc gcgatttact tttagagcta aaagcggaag gatgatgaac
     1021 aaatggctat ggatcggata gaggtgaagg gagccagggc gcataacctg aaaaatatag
     1081 atgtaacgat tccgagagat cagcttgtcg ttgtcacggg tttgtccgga tcaggtaaat
     1141 cctcccttgc ctttgacacg atatatgctg aaggacagag acggtatgtc gagtcgctgt
     1201 ctgcttatgc ccgccagttt ttagggcaaa tggataagcc tgatgtggat gcaattgagg
     1261 ggctctctcc cgccatcagc attgatcaga aaacaacgag ccgcaatccg aggtctactg
     1321 tcggtacggt aactgagatt tatgattatc tgcgtctttt atatgcgaga gtagggaagc
     1381 ctcattgtcc ggaacacgga attgagatta catcccagac catcgagcaa atggtggaca
     1441 gaattctgga atacccggaa cggacgaagc ttcaggtgct ggcgccgatt gtctcgggcc
     1501 gaaaaggcgc tcatgtcaaa gtgcttgaac agattagaaa acaaggctat gtcagagtca
     1561 gaattgacgg cgagatggct gagctttccg acgatatcga attagaaaag aacaagaagc
     1621 attccattga ggtagtcatt gaccggattg tcgtgaaaga aggcgtggca gcccggctgt
     1681 cagattcatt ggaaacggcg ctccgtttag gtgaaggacg ggttatgatc gatgtcatcg
     1741 gtgaggaaga gctgatgttc agcgagcatc atgcctgtcc gcactgcgga ttttcaattg
     1801 gtgagcttga gccgcgtctg ttttcgttta acagtccgtt cggggcgtgt ccgacgtgtg
     1861 acggtctcgg aatgaagctt gaagtggatg ccgatcttgt catccccaat caagatttgt
     1921 cattgaagga gaatgcggtc gtcccttgga caccgatcag ctcacaatat tatcctcagc
     1981 tgcttgaggc agtctgcacc cactacggga ttgatatgga tgtgccggtc aaagatttgc
     2041 cgaagcatca actggataaa gtgctgtacg gcagcggaga tgacctgatt tatttccgat
     2101 atgaaaatga ttttggacaa atccgcgaag gtgaaattca atttgaaggc gtattgcgca
     2161 acattgaaag acgctataag gagacaggct ccgatttcat acgtgagcaa atggagcagt
     2221 atatgtctca gaagtcttgt ccgacgtgca aaggctatcg gttaaagaaa gaggcgcttg
     2281 ccgtactgat tgacggccgc cacattggaa aaattaccga gctgtctgtc gcagacgcac
     2341 ttgccttctt taaagacctt accctttctg agaaggatat gcagatcgcc aatttgattt
     2401 tgcgcgaaat tgtggagcgc ttaagctttc tggacaaagt cggtctcgat tacctgacat
     2461 tgagcagggc agcgggtaca ttgtccgggg gagaggcgca gcgcatcagg ctggcgactc
     2521 aaattggctc gcgtttatcc ggtgtgcttt atattttaga tgagccgtct atcggtctgc
     2581 atcagcgtga taacgaccgc ttgatcagcg ctctgaaaaa tatgagagac ctcgggaaca
     2641 cgctgattgt tgtcgaacat gatgaggaca cgatgatggc agcagattat ttaatagaca
     2701 ttggaccggg agccggcatt cacggcggac aggtgatatc tgcgggtacg ccggaagaag
     2761 tgatggaaga tccaaactca ttaacgggca gctatttatc agggaaaaag tttatcccat
     2821 tgcctcctga aagaagaaag ccggacggac gttacattga aattaaaggt gcttcagaaa
     2881 acaacctgaa aaaagtgaat gccaagttcc cgcttgggac gtttacagca gttacaggtg
     2941 tttccggttc aggaaagagt acactcgtta atgaaatttt gcataaggcg ctggcgcaaa
     3001 agcttcataa agcgaaagcg aagcccggca gccataaaga gattaaaggt ttggatcatt
     3061 tagacaaagt cattgacatt gaccaggcgc caatcggaag aacgccgaga tccaaccctg
     3121 cgacatacac cggtgtattt gatgacattc gtgatgtatt cgcgcagaca aatgaagcga
     3181 aggtccgcgg ctataaaaaa ggccgtttca gcttcaacgt gaagggcgga cgatgtgaag
     3241 cctgccgcgg agacgggatt attaaaattg aaatgcactt ccttcctgac gtatacgttc
     3301 catgcgaggt gtgtcacggc aaacgctata atcgtgaaac gcttgaagtg acgtacaaag
     3361 gaaaaagcat ctctgatgtg cttgatatga ctgttgaaga tgctctttct ttctttgaaa
     3421 atatcccgaa aatcaaacgc aagctccaaa ccctttatga tgttggttta ggttatatta
     3481 cgctcggcca gccggcgacg accttgtcag gcggagaagc gcagcgcgtg aagctcgcat
     3541 cagagctgca caaacgctcg accggacgca cgctctacat tttagatgag ccgacgacag
     3601 gtttgcatgt cgacgatatc gccaggcttc ttgtcgtgct gcaacggttg gtagacaacg
     3661 gagacactgt actggttatt gagcacaatc ttgatatcat taagacggcc gattacattg
     3721 tggatttggg cccggaaggc ggagccgggg gcggaaccat tgtcgcgtca ggaacgcctg
     3781 aggaaatcac tgaagtggaa gaatcgtata caggccgtta tttgaagcct gttatcgaac
     3841 gtgacaaaac acgcatgaaa tcgctcttaa aagcgaaaga aacagctaca tcttaaaaac
     3901 tttattacga gagaacagct tgtcagcaag tccatccttg ggcttagcag gcaagctttt
     3961 tctttacggc ataataggtt tagaatcgtc tttgggtaaa atttctttgg gaaaacgcct
     4021 aaacatttac aagttgctgt cgatatatga attgaaaggc ggatgatgat ttccaaatta
     4081 agcagataaa ccctattaag gggcttaaag gcatccttaa gcaataactg aatctacata
     4141 ggttagaagc tttattggag tctgatacgc cagaaatcaa agaaaaggaa gtctttgatt
     4201 tcaaaaacat atacacatgg aggtattttt cacatgagaa tcaaccacaa tattgcagcg
     4261 cttaacacac taaaccgttt gtcttcaaac aacagtgcga gctcaaaaaa catggagaaa
     4321 ctttcttctg gtcttcgcat caaccgtgcg ggagatgacg cagcgggtct agcgatctct
     4381 gaaaaaatga gaggacaaat cagaggtctt gaaatggctt ctaaaaactc tcaagacgga
     4441 atttctctta tccaaacagc tgagggtgca ttgactgaaa ctcactcaat tcttcaacgt
     4501 gtccgtgagc tagttgttca agcaggaaac acaggtactc aagatggtac tgaccttcaa
     4561 gccatccaag acgagatatc ttcacttgtt gaagaaatcg gtggcggaac aaacgctaac
     4621 ggtatctctg accgtactga attcaacggt aagaaactcc ttaacggtgc tttctcagaa
     4681 gatagtggta aatcactaac attccaaatt ggtgctaacg gtagccagca gctttctgtc
     4741 aacattgaaa gtatggccgc aacagcaatc ggtgctctag atgatgatgg taatcttgtt
     4801 gctgatcaaa cagtagctaa aatcgatgta accaaatttg ctgataatgc agctgacact
     4861 gcaactgtag gattcgatgc tcaacttaaa acagttgatg acgctatcaa cactgtttct
     4921 aaacaacgtt caaaattagg tgcggtacaa aaccgtctag agcacacaat caacaactta
     4981 agcgcttctg gtgaaaacct gactgctgct gagtctcgta tccgtgacgt tgacatggcg
     5041 aaagaaatga gcgagttcac aaagaacaac attctttctc aagcttctca agcgatgctt
     5101 gctcaagcaa accaacagcc gcaaaacgtg cttcaattgt tacgttaatt ttgaacggct
     5161 atctctttct gatagttaca gcttgccgac agagtccatt aactggatgt gccggcaggc
     5221 tttttttgtt acaatcattt tgtgacagcc agggaatcac ttgttttttc aaaacaaaaa
     5281 aggcaagata ttgtcatcta acctttaact ttgtttcagt tataatgaat gatatgtttt
     5341 ccgcaattac aaagtcttct tatacagctg cttacttaca ctcattaaat cttgaacaat
     5401 atgcgtgttg ctgctgtcga gcagcacttt tatatattgg atatcgtgag agatcgggaa
     5461 gggtccgtga atctgctcaa gctcggcgat catttcacga tttattcttt tttgttcatg
     5521 aatcagcttt aacactgatt cttgtgttac tgtatagtca ctgggatctt tctccattgc
     5581 ttgtccagcc tccttggttg ccgtaagctt tttacatatt gtaatcggta tagccggaag
     5641 attctacttc taaaactttt taaaacccaa tttccgtaaa tcggagcatg tctaaagaat
     5701 gaatttgaaa cctgaagaga ttttaaacgt ataaataagt aaaacgtccc tgatcttttg
     5761 tcatggacaa ttcacaatag gagtgtggaa tgaattgaac cgtaatcaag ctattattgc
     5821 atcactttgt tatttcagtg tgtttattgc gccaatcatt gtgccgattg tcgcttattt
     5881 tgttgtaaat gagaaagaaa caaaacgcca tgcgatacgc tctcttattt ctcacatagt
     5941 tccgtttgtc ggctggctgt tcctttttat tgctttactt ggcggagccg ttgcgattga
     6001 tggagacagc ttgcttcctg tatttgtaat catcggggga gcggttattt atttcttagt
     6061 tgtcattggc attatcattt ggaatgtgat tcagggcatt aaggtcctgc gtgaagcata
     6121 agtagacaat ggggaggaga tcgtaatgaa gcaagaaaag gaacgaatcc ttaagcttgt
     6181 tgaggaagga aagcttacag cacaagaagc tttgacactg attgaaaagc ttgacagcga
     6241 ctacaaggaa aaagaagaga aaatcacagc gctctcagtt cacgttcatg acgaggaaga
     6301 gccatttaca acggcaaaaa aagaaagcgg caagccgtct ttaggagcca agctgtttga
     6361 ctggattgat tctgctgtaa aaaaggtcaa ggaagttgac cttgatttaa actttggcca
     6421 tgcgtatgat gttcagcaca tttttcaatt taaagatact gacttttcca gtgttgagct
     6481 acaaattgcc aatggcagcg tgaatatcgt tccttgggaa gatgacgata tcagagcgga
     6541 atgccaggcg aaggtatatc gcgctgatag ccaggatgcg gcgagacacg catttcttca
     6601 gcatattgaa tgtgaaatca aaggaaataa gttttttatc cgtactgaga aaaagacgat
     6661 gaaaacgaat gtcactttgt atattcctca gaaggagtat gataagattc gagtgaagct
     6721 gtttaacggt ccggtcagag gggaacattt acatgtaaaa gagttctcag cgaaaacgac
     6781 aaacggcgtg ctgtcgttct cgcacttaac agccgaaaaa gccatagctg aaacggccaa
     6841 cgggcaaatt aagctcgcca gccacagctg cggaacgata gaagcggaaa ccattaacgg
     6901 attgatcgat ctcagaggga aaagcgaatc cattgacgtt caaagcttta acggaaatat
     6961 tgcgattaac gtaacagaat cagactgccg ctccatttat gcaaaaacaa cgactggaag
     7021 tgtggagctt gcgattccgg acgatctcgc agtgaaggca gagctgaaaa gcaatcttgg
     7081 ttcgctgtcc catgaattaa tggatgttga gatgctgaag gaaaaaaatg ataccattca
     7141 aaaagaaatg atgttcactt caaatcaggc acatgatcaa aacatcaccg tcttctcaga
     7201 atcactaaca ggcgccatca aactaaagta ctcacagagg tgatcgaatg aataagcttt
     7261 atcgctcaga aaaaaataag aaaatcgccg gtgttatcgg aggtctcgcg gaatatttca
     7321 attgggacgc atcactcctc agagtcataa ctgtcatctt agccattatg acaagtgtct
     7381 tgccggtttt gctcatttat atcatttgga tttttatcgt cccgtcagaa agggatatga
     7441 aatgacatgg taaaatgggc agtcagcatt ttagtcaatg cattgttatt aatcgtcatt
     7501 gatggatata ttgactccat ccatatcagc agcattgggg cagcgattat cgccagcctc
     7561 attttatcga tcttaaacgt gctgattaaa ccgcttttaa ttatctttac actgccggtc
     7621 accatggtga cgcttggact cttcttgttt gtgattaacg ccatcacgct tatgatgacc
     7681 gcgtccatta tgggtgacag ctttcaaatc gacggcttcg gcacagcgat atgggcatcc
     7741 gttatcctgt cggtcttcca tttgctgatt caaaaaggca ttttagagcc gcttagaaaa
     7801 aaataaaaaa agctgcccgc aaaggcagct tttttacatg tggtgctttt tttgtttcgt
     7861 gcgcttctgc ggcgaaaaca ttggcggaag aaggcaaaag ctcaatgcgg caaatccaag
     7921 gcctaggagg ctaaaggaca tggcagacgg catcattgtg agcaagtagg cagataccat
     7981 cggacctgct gccatgcctg tatatcggat aaagttaaac attccgattg cactccctct
     8041 gttgtgctca aattcattag taatcattgt agagtacaag ggaggaataa cccccatcga
     8101 gatgccaaac agtgccagcg tcagagccat gagaacgaga gaaacggaat gtgtaacagc
     8161 aaataaaatg atgctgcagg cggcaatcac attgcttccg ataaacaagg tgtgcagccc
     8221 gatttttgcc tgtattcttt taaacagaaa cgtacctgca atcgtgctca gcgccagcgg
     8281 caaatataac agtcctgcta tacccacatc tatatggtaa tgctctgtca gcaatattgg
     8341 gaggtagaca attactgcaa aataaatgaa aaagagaaca aaacttaaag tcaaaatgac
     8401 gttccctgtt cgatttgtaa atattgattt ataatgagcg aacacattgc ctttggcttg
     8461 ctgcatagat tcagttggag aatctttagg aaaggtgatg ctgtttgtca ccagcaaaac
     8521 gatcgagatg gccgcaagta tccaaaaaat cccttcgtat ccggctgctc cgcctatgaa
     8581 tcctcctaga actggggcaa tagccggtgc gacagacagg agcatttgat acgttcccat
     8641 tgcactcccg cgttcattgc ctgtaaacag ctgtccgatc gttgtggcag caataagagg
     8701 cagtgctgcg gaaccggctg cctgtatcat tctgaatatc agaaacaaag taaagtcagt
     8761 agtcaccgca cagccgatgc tggctgctgc cgttgccaga ataccggtaa tcaagacgat
     8821 ccgagcgcct ttaaaatcaa tgatcgctcc taatataatt tgcattattg ctgtcacaat
     8881 cataaaaact gagactgaca ggttcaccat agctgtggaa acatggaatg attctttaat
     8941 gatcggaaga ataggtgaat aaatgttctg gcttaaagat gcaaaaaatg cactgaagca
     9001 aacagtgtat aagatcaata ctgcctttga ttttgtatga gccaaagtgt acaccgcctt
     9061 tttgtttact ttacaggttt gtccggagtc ccatcggttt gttctgcttc aagtgttgac
     9121 gtcaactgct ctagaaactt caatacagcc ttttgttctt cttgtgaaaa tgaatcgagg
     9181 aattggtaaa aagccagctc ctttttttta tgcagtttct catgtaagtc gaaaatccgt
     9241 ctgcctttac gggttaattt aaaataaact tcttttttgt tatctgtcag ctgatagctg
     9301 ttaatgaatt cttccttgat cagttttgtg ctgattttcg ttacattcgc tttcgataag
     9361 ttcatttttc tggcaatccc cgcattgtta atgggttcat gctggccgat gcagtcaatg
     9421 acgtgaatgc ttgtcatatt tttgggaatt tcagctacat cctcttctgc ggcctcctta
     9481 aaaaattgct gaagtgaagt gttcgctttt tgttcattaa ggtgcagcag tctcacgtat
     9541 atgtcgtata tcatctgttt tgtcaaatca gacataggct accttctttc ttaggaataa
     9601 tagtttacga tgaaacaata aaacaaatcg tttactggtg aacaatttta ttttatcttt
     9661 tcttgttttc gttgtcaatt gttggtgttt agactatttg ttgatatgtc atgctgaata
     9721 tgcgtgtaaa aagtgtgtgg aaaatgtgat gtttacattt attttcaaat atttgggcac
     9781 agatcatatt gacgtttgga aggaaaaggg ataaaatgac taatgtttac tagtaaacat
     9841 ttatcatgat atgggtattg aaaatgatta ttaaaatctt aaaaaacatt tggaaacttt
     9901 aagtgatgcc tgccattcgt cttgttcagt tgttctagct attccatctt gtatttgagg
     9961 gtgatgactt aaagcgggat aagttttagg ggtgaatgag atgaccggaa tggtaacgga
    10021 aagaaggtct gtgcatttta ttgctgagcc attaacggaa aactgcagag aaatatttga
    10081 acgccgcagg catgttttgg tggggatcag cccatttaac agctggtttt cagaggatta
    10141 tatttacaga ttaattggat gggcgaaagc tcaatttaaa agcgtttctg ttttacttgc
    10201 ggggcatgag gccgctaatc ttctagaagc gcttggaact ccgagaggaa aggctgaacg
    10261 aaaagtaagg aaagaggtat cacgaaacag gagatttgca gaaagagctc ttgtggctca
    10321 tggcggggat ccgaaggcga ttcatacatt ttctgatttt atggataaca aagcctacca
    10381 gctgttgaga caagaagttg aacatgcttt ttttgagcag cctcattttc gacatgcttg
    10441 tttggacatg tctcgtgaag cgataatcgg gcgtgcacgg ggcgtcagtt tgatgatgga
    10501 agaagtcagt gaggatatgc tgaatttggc tgtggaatat gtcatagctg agctgccgtt
    10561 ttttatcgga gctccggata ttttagaggt ggaagagacg ctccttgctt atcatcgtcc
    10621 gtggaagctc ggtgagaaga tcagtaacca tgaattttct atttgtatgc ggccgaatca
    10681 agggtatctc attgtacagg aaatggcgca gatgctttct gagaaacgga tcacatgtga
    10741 aggatgatag ggggagtaaa acatgagcca atcgattaaa ttgtttagtg tgctttctga
    10801 tcaatttcaa aacaatccat atgcttattt ttcacaactg cgggaggaag atccggttca
    10861 ttatgaagag tcgatagaca gttattttat cagccgctat catgatgtcc gctatatcct
    10921 tcagcatccg gatattttca cgacgaaatc acttgttgag cgtgccgaac cagtaatgcg
    10981 aggccctgtg ctggcccaaa tgcatggaaa agaacactct gccaaaagaa gaattgtagt
    11041 gagaagcttc atcggtgacg cactggatca tctgtctcca ttgattaaac aaaatgcaga
    11101 aaacttgtta gcgccttatc ttgaaagagg gaaaattgat ctcgtcaatg attttggaaa
    11161 gacgtttgcg gtgtgcgtca cgatggacat gctcgggctg gataaaagag accatgaaaa
    11221 aatctctgag tggcacagcg gagttgccga ttttatcacg agtatctctc aatctcctga
    11281 agcgcgggca cattcgttat ggtgcagcga acagctttcc caatacttga tgccggtcat
    11341 tgaagaacgt cgcgtcaatc cgggatcaga tttaatttcc atcctatgta cttctgaata
    11401 tgaaggcatg gcgatgtcgg acaaggatat actcgcactg attcttaatg tgctgttagc
    11461 cgcaacggaa ccggctgata agacgctggc actgatgatc taccatttgc tcaacaatcc
    11521 tgagcagatg aatgatgttt tggctgaccg ttcgttagtt ccgagagcca ttgcggagac
    11581 attgcgttat aaaccgccgg ttcagctgat tccgcggcag ctgtcccaag atacagtggt
    11641 cggcggtatg gaaatcaaaa aagatacgat tgttttttgt atgatcggtg cggctaaccg
    11701 ggaccctgaa gcatttgaac agcctgacgt gtttaatatt catcgggaag atcttggcat
    11761 caagagcgct tttagcggcg ccgcccggca tctcgctttc ggatccggca ttcataactg
    11821 tgtaggagca gcttttgcca aaaccgaaat cgaaattgta gctaatattg tgctggataa
    11881 gatgcggaat atcagattag aggaagattt ttgttatgct gagtccggtc tgtatacacg
    11941 cggacccgtg tcacttcata ttgtatttga cagggcttaa taatatggaa aggtctctcc
    12001 cgtgcgggtg aggctttttt acatatgctt gatccacaag ttaataaacg cagtcactgt
    12061 tttcaactca tcaattgaaa attcattgaa gagctgtttg tattgctcct ttgctttttg
    12121 atgaagccgt tcatgtaacc ccgccagttc tttgccgctt tcagttaacg tataaaaaat
    12181 ttcttttttg ttgcccggct tttttgtcac tgtaatgatg ttatgtttca acaaagcttg
    12241 aattgctttt gaaacagctg cttttgagat atgtaagcgg gatgctaaga agctgttatt
    12301 cacatttttg ctggtatgta aacaggaaat aatgtgaagc tgggtcaatg tccagtcttt
    12361 ttgaggtggt cgagatgttg tgaggacgga atctgcacct tttgccagac gcgtttatct
    12421 ctttttaatg cgaattgttg tatagcttga aataattcat catgaatgct gttgttcatc
    12481 aatttgcctc tctgatttaa ttgttaactg gttaactatc cttagtttac tggggaatag
    12541 tggaattttc aagaggcaga cgtttttttg ttctttatgc acaaaaatga actcataagc
    12601 ttccctgttc agacgcaaaa tacatgaaaa gggaggtgct ttgatgagga aatacacggt
    12661 tattgtttct atattactgt cttttctttc cgttttgtct ggcggccatc atgaatcgaa
    12721 agcttttcct gttgtacagc aggaactatt tcaaacgcct cattacatac ccttgcttga
    12781 aaatcctcct caaattgatg ttaaagctga aatgagaaac aatctaatga ttgaggctca
    12841 agttcgcgat aattcgtatc aagcattttc cgcctttctt ttttataaac aaagtaatga
    12901 gctgggctac aaaatggtac cgatggaccc gacccccgga gcggtccgca agtttttggc
    12961 acaaattccg aaacgtttga tttggaacag cgagcttgag tattacattg tgatgtcgaa
    13021 tgggaagcag cgggtggagt cggagacgaa gaaactgaag cttgaaggat accaggctga
    13081 ccttgcccat attccggagc ttctgatcac agagctggcg gttgatacga agaatatagg
    13141 acgtgcagac ggttatgaat ttattgagat ttttaatacg actgacagaa cgattgattt
    13201 taaagactac catatccgtt accgctaccc aaaagaaggg cctgactccg atctgatttg
    13261 gagacctgat gaaaggatct tgataccatc cggcgaaacg tttgtcgtct ggctgaagcc
    13321 tgccgggcat ccagaattga cgtcagagga tttcaatcgt tattatcaga cacaacttaa
    13381 agaaggaaaa aatctcgcgg tgatagacga gacagagggt atggccaata cgaggccgcg
    13441 ggccgttgtg atatcaacga atacaggaaa ggacatttcg gttgcgcatt atcggaaaca
    13501 tgcgctgcgc cggctgtcat cagtgcttta taagtatccg ttgaacggaa ccgcagaact
    13561 tctgaacatc tcaatcgggg aaaagaatcc gtctcccgga accgtattac aggcacaggt
    13621 gcccgagcag aaacgaaaaa tcaagctcga taaagaaaag ccggtcattg aggatttgac
    13681 tgaccgcaag cctgtccgcc ctgctgaaag cattgagctg cgggctgaca tacgggaccg
    13741 aagtctagtg aaaacagcgg ccttttacta ccggacggat gagaacaaac cgtttaagcg
    13801 aattcttgct gaaaaagacc gcaacgataa tctctaccat tatattgtgt attcgcctga
    13861 attgatcggg aaggatcagc tagagtatta tgtggcggcc ggggatggga tcaatgaagc
    13921 gagaacgccg gtcaaaatga ttgatattaa acaaacaagc aagtcgcacg gtctgcggat
    13981 gaatattgaa aacagagaca cgctgtcggg aactcaattt ctcaaagcca cgacagaagg
    14041 gcgagctgac cgcattaagc tatggattga cgggaaaaaa caagtgacag agcccgcgat
    14101 ggaaaaagaa gtatattttg cgttcgatac gaggaaaacg aacctttatt ttaaaaacgc
    14161 ggtaacgatg gaaggcaaag tgctgaaggt gttcgatgat acaacaaata aataccgcac
    14221 ctattctgtt ccattgccag agacactgct tcggaaaggg aagcagctgc agcgtattac
    14281 aatacgatcg ggttcgaagg tgtctccgtt tgatacggct gaaaaccgtg atgacttctt
    14341 agtcaaaaac gcccgattgg tgttatcgga tggaacggtg atcagggatc agagggtgtc
    14401 tcctgagaag gaactcttta ttggcgataa tcagcgctcg aataaaagct ggcaatttca
    14461 gttcaatctg cctgacggac tgtttacatc acagctgctg gagtgggaca catcaaagct
    14521 ttctgaaggc gctcaccata ttcaagcaag cgacggcaag gaaaatgtca gcctggttgt
    14581 acgggtagat aattcaggac ctcacattga gccgaatatc accgaggggc aaacgtataa
    14641 aggaaacctt atcctgcagg cggatatgta tgataaatgg agcaaaattg aggaagcgga
    14701 agcgtcttta gatggagaaa gcattacact cccatatcat acgtcctctt ctgatctttt
    14761 gccgggaaaa cattctctaa aagtgacagc aacggatctc gcgggcaata aaacagtgat
    14821 tgagaggata tttaaaaccg aacgagagca tccggaccag ccggaggtta tagacagcga
    14881 agccgacacg cataaggcca agctttctgt acgggtgaaa gatccgacga atgatgccat
    14941 ggatgttggt ttttataggg gatttcaata tacagcgcga aatcatgtga aaatatttaa
    15001 gcacgcgtca ctgactgaac cgccgaaaag ctttgtgccg gaaagtgaga ccccatttac
    15061 gaataaagaa ttggagcggg tatctgctgc tgatggaaaa acagtatcga cagaaaacaa
    15121 agagcttttc ccttatcatc gctttgaagt gacggttgat ccatcaattg atgagaatga
    15181 tctggcagaa gcggtctgga agggcagttc tttgccgggg agaaaggtga cgatgtatgc
    15241 ttggaattat cggacaaacg aatggcagcc tgttgacagc ttcgtcgcga aggatgacaa
    15301 accgtttacg ctgaaagcgt ctgtgattgc cgccgatttt gtacgggagt cgaaaatgaa
    15361 tgtgattgta caggatgaaa ttcctcctgc gaaggatatg tacacatttg tctggatgtc
    15421 tgatacacag tattacgccg aaagctatcc gcatattttt gataaacaga ccgaatggat
    15481 aaaagataat caaaagcagc tcaatatcaa atatgtgttc cacacgggtg atattgtaga
    15541 tgattccgct gatatcagac agtggaaaaa cgctgatcgg tcaatgtccg ttctggataa
    15601 gagcggaatt ccttacggcg tgctggccgg caaccacgat gtggggcata aggacggctc
    15661 ctacagggcg tttggcaaat attttggcag cgatcgtttt gataaaaaat tgcattatgg
    15721 cggttcctat aaaaataacc gcggacatta tgacctgatc tcgagcaacg gcaatgatta
    15781 tatcatgctt tacatgggat ggggcattac ggatgaagac atcgcgtgga tgaatcaggt
    15841 gttgaagaaa caccctgacc ggatggccat tttagctttt catgagtatt tgctggtcag
    15901 cggaaacaga agcccgatcg gagagaaaat attcaaagaa atcgtaaagc cgaacccgaa
    15961 tgtcgtgatg gtattgagcg ggcattatca tagtgcgatg agaaaaacag atgagctgga
    16021 tgatgatggt gatgggaagc cggatcgtct tgtgcatcaa atgctggccg attatcaggg
    16081 cgggccagaa ggcggtcaag ggtatttgcg tttgcttcaa tttgaccagg ccaatgacat
    16141 ggtgcacgta tcgacgtatt ctccgtatgt aaaggataag aattactatg atacggatac
    16201 ctatggcaac aaggatgaat tttccctttc gcttgatttg aagccaagaa tcaaaaaggt
    16261 ggaaaccgat tattttgaat gtaacgtata taccaatgaa gagctcggaa aacgggagca
    16321 agtgaagagc ggggatacag ctgaattccg ctgggatcat cttgaaccgc agtctgttta
    16381 ttattggtac attatagtgg aagacagttt taacggcaaa acgaaatcac cgatctggaa
    16441 gttcaagacg aaaaaagaaa catatcgtcc ggcaccagac cagtttgatt ttcgccatgt
    16501 ctccaatcct taaaaagggt tggaggcttt ttttatgaaa gacgatccat atagtggaca
    16561 aatgtataac ggtcgccact gtatacggat tttgcatgct caaaagcagt tccgttctgc
    16621 agatatgttg ttcgtttaat taataggact ggcgctccct tttgaatacc aagaatgttc
    16681 gcttcttccg tggtggcagc gcttggctca agctcctgtt ttgcacggga aatcggtatg
    16741 ctgttatacc tttcaatatg atcatagatc gacgactgaa aatgcgattc gttcaattca
    16801 cccgcaagct caaacggaat atgtgaggac tcaatcgcca tcggaatatc atttgccagc
    16861 cgcacccgag tgattttatg gatagaggag gggtgcccgc agcctaatat agccgcgagc
    16921 tcctcagttg aatcaataag ctgataatca atgagcctgc tgcccggtgt catcccgcgg
    16981 cttttcatat cctcggtaaa gcttgtcagc ccttgaagtg cttgttccat ttttggcttg
    17041 ctgacaaagg tgccccgccc cttcaggcga tagagcaagc cttcattaac taaattagaa
    17101 agcgcctggc gaactgtcat ccggctgatc ccgaattgtt cggcatattc gcgctcagaa
    17161 ggaagaggca tatccggctg cagttctccg ctcttaattt gggtttttaa ttgctccata
    17221 atctgataat aaatcggaat aggcgattgt ttattgatat tcataagtca gcatgttcct
    17281 ttcaaggtct taatgacgcg gcttcacgat caatcaaaac cgtgacatcg gaatgcaggt
    17341 gcaaagcaga tgcgggaaaa tcttcgctta tgtttccttc taacagctta cgaacagctt
    17401 ctgctttgct tttcccagaa ataagcagca gaatgcgttt gcttgagaga atcgttcgaa
    17461 ttcccattgt cagtgctttt ttcggcacgc tgtctatgga aggaaaatat ctggcattcg
    17521 cttggcgggt ttgctcattt aatgtcacaa catgagttcg cgacttaaaa gaggttcccg
    17581 gttcgttaaa gccgatgtgt ccgtttcggc cgatgccgag aagctgaatg tcagtgccgc
    17641 cgagggactc gactagctgt tcatagcgtc tgcattcggc ttccaaatcg tctgcatttc
    17701 cattcggaat aaaatgccgg ctaggcttgc tgtcgatatg ctgaaataaa cggtcattca
    17761 tatagaagtg atagctgttc gggtcattgc ttgaaagtct ggcgtactca tccaaattaa
    17821 ctgtagtgac gttttgaaat gagagattct cagtttggtg caggcggatc agctcgcgat
    17881 aagtgcctta cggtgtgccg cccgtcgcta acccgagaac ggcatcaggt ttttctttga
    17941 ttgtatctgc cgttattctg gctgcttttt ggcttagctc ttcatacgtt tgacattcca
    18001 ttactttcat atctggtcag cctccttgga tataaatgca atgtttccgc ggcaaatggt
    18061 gagaatcacc tcgcaatcac tgctgacaat caccaaatct gcatcttttc ccacagtcac
    18121 actgcctttt cggtcaaaga tgcccagctg tttggccgcg tttgcagaag ttatattagc
    18181 tatatccgtc caagagcaat ttgtaaactc acgcatatgc cgtgcacctt cgttcatttt
    18241 gaggatcgaa ccggcaaggg ttccgtctga aagaagagct gttcgtcctc ttaccgtcac
    18301 actttggccg ccgaactcat aaacgccgtc tttcagtcct tttgccctca tggaatcggt
    18361 aattaaaatg agcttgctgc tgcctttcgc caaaaaagca agcttagccg ctaaaggatg
    18421 ggagtggatg ccatcggcta ttagctctgt cacaaaccca tcatgggcca gcgccgttcc
    18481 gatcactccg ggttctcggt gatgaaacgg gctcatcgcg ttgtacagat gggtcatatg
    18541 gctcgcccct gcttttgcgg catccgacag cattgcggaa tcggcatccg tgtgccccat
    18601 ggacgcgata atcgattctt ctttcaaatg gcggatcagt tcaaaatgct gatcctcctc
    18661 cggcgcaagt gtaacaattt tgatcagtcc gcccgcctcc tgctgccact ttttgaaaag
    18721 cgcaacatcc gaaggcctaa tccactcttt tggttgcgct ccggctcttt taggtgaaac
    18781 aaaggggccc tctaaatgaa tgccgagcag ttctgcacct aataaagagg actcttcagc
    18841 cgctttccac tctcttgcgt tcaccaaagc ttgagaaatg ttcccatgtt cctgggtaat
    18901 cgttgtggct aaaaagctcg tcgtgccttc ttcgggaagt cttgacgaca tgatgtcgag
    18961 cgcagaaaag cttgcatcca tcgtatctgc gccatagccg ccgtggatat gaatgtcaat
    19021 catgccggga agcaaaacgg aattcgcagg tgcttgaatt tcttttgaat atgactcctt
    19081 gggccgttct gttgagactg tgctgatttt tccgtcattg attccgacat agccgttttt
    19141 gattacttca ttttctgtca cgatcgcaat gtctttgata agaagactct ctgccataga
    19201 tgatccgcct ttctggaatt tgaatacctt tattttactc tttttcagac tagtgatcta
    19261 gaccagctga ggtgttttcc cgccttttat aaaatctgtt ttaggttcaa gcctgaaagt
    19321 gataaaatga aagtattgtg agcaagctcg gggcagcccg gaacaggagg aacatatcgt
    19381 ggcaaaggtt cgcacaaaag acgtaatgga acagttcaat ttggaattaa tcagcggaga
    19441 agaaggaatt aaccgcccga ttacaatgag tgacctatca agaccaggca ttgaaattgc
    19501 cggatatttt acatattacc cgagagagcg cgtgcagctt ctcggaaaaa cagagctttc
    19561 tttctttgaa cagcttcctg aagaggagaa aaaacagcga atggattctc tgtgcactga
    19621 tgtaacacca gctattattc tttctagaga tatgcccatc ccgcaggagc tgattgacgc
    19681 ctccgagaaa aatggggtgc ctgtcctcag atcgccgctg aaaacgacaa gactgtcaag
    19741 ccgtttaacc aacttccttg agagcaggct ggcgcctacc acagcaattc atggcgttct
    19801 tgtcgatata tatggtgtcg gcgtgctgat cacaggaaag agcggcgtcg gaaaaagcga
    19861 aacagcgctg gagcttgtga aaagagggca tcgccttgtc gccgatgatt gcgttgaaat
    19921 tcggcaggag gatcaggata ctcttgtcgg aaacgctccg gaattaattg agcatctcct
    19981 tgaaatcagg gggcttggca ttatcaacgt gatgacgcta tttggcgctg gcgcggtcag
    20041 aagcaataaa cgaatcacga ttgtcatgaa tcttgaactt tgggagcagg gcaagcaata
    20101 cgaccggctc ggacttgagg aagagaccat gaaaatcatt gatacagaaa tcacaaaact
    20161 gacgattccc gtccgtccag gccgaaatct tgcagtcatc atcgaagtgg cagcaatgaa
    20221 cttcagattg aagcggatgg ggctgaatgc cgctgaacaa ttcaccaaca agctggcgga
    20281 cgtcattgaa gacggtgaac aagaagaata ggagttggcg ttaaatgaat gaagcgatag
    20341 aaccactcaa tccaatagca tttcagctgg ggccgttagc cgtccattgg tacggaatca
    20401 ttatcggtct cggcgctttg ctcgggctgt ggattgcgat gagggaaagt gaaaaacggg
    20461 ggctgcagaa agatacgttt attgatcttg tcctgtttgc cattccgatt gcgattattt
    20521 gcgcgcgaat ttattacgtt gcctttgaat gggactatta tgcggcacat ccgggagaaa
    20581 tcattaagat ttggaagggc ggcattgcca ttcatggcgg attaatcggc gccattttaa
    20641 cgggttatgt gttttcgaga gtgaaaaaac tttcgttctg gaagcttgcg gacattgccg
    20701 cgcccagcat tttgctcggc caggcgatcg gccgctgggg gaacttc
//
