LOCUS       NZ_CP099738            10819 bp    DNA     circular CON 27-AUG-2024
DEFINITION  Lentzea sp. HUAS12 chromosome.
ACCESSION   NZ_CP099738
VERSION     NZ_CP099738.1
KEYWORDS    RefSeq.
SOURCE      Lentzea sp. HUAS12
  ORGANISM  Lentzea sp. HUAS12
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Lentzea.
REFERENCE   1  (bases 1 to 9676923)
  AUTHORS   Ping,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2022) College of Life and Environment Science,
            Hunan University of Arts and Science, No. 3150 Dongting Avenue,
            Changde, Hunan 415000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024125735.1-RS_2024_08_27
            Annotation Date                   :: 08/27/2024 06:34:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,161
            CDSs (total)                      :: 9,080
            Genes (coding)                    :: 8,988
            CDSs (with protein)               :: 8,988
            Genes (RNA)                       :: 81
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 21 of 92
            Pseudo Genes (incomplete)         :: 77 of 92
            Pseudo Genes (internal stop)      :: 3 of 92
            Pseudo Genes (multiple problems)  :: 8 of 92
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:47:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4847166
            Orig. end                         :: 4857985
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP099738.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Ping Mo.
FEATURES             Location/Qualifiers
     region          1..10819
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="24"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10819
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10819
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5819](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5819
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            314..604
                     /locus_tag="ND450_RS21385"
                     /old_locus_tag="ND450_21385"
     CDS             314..604
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800404.1"
                     /locus_tag="ND450_RS21385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ND450_21385"
                     /product="hypothetical protein"
                     /protein_id="WP_253320571.1"
                     /transl_table=11
                     /translation="MGDSKDESEKFQEELIGLDPHDPETKAFADHLDRMQRQRPGYTVE
                     GYLQGVQDFADSANRTTGHQRLVAVIVVGLILLGVILTIWFALGDIIAIIF"
     misc_feature    complement(356..369)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.84"
                     /tool="antismash"
     misc_feature    953..969
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.87"
                     /tool="antismash"
     gene            968..1390
                     /gene="msrB"
                     /locus_tag="ND450_RS21390"
                     /old_locus_tag="ND450_21390"
     CDS             968..1390
                     /EC_number="1.8.4.12"
                     /GO_function="GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422371.1"
                     /locus_tag="ND450_RS21390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ND450_21390"
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="WP_089923159.1"
                     /transl_table=11
                     /translation="MEPVVGATPKVVRSEEEWRAQLNPAEYAVLRQAGTERPWTGEYTE
                     TKTEGLYECRACGAELFRSDTKFDSHCGWPSFFSPLAGDSVILREDRTLGMKRVEVLCA
                     TCHSHLGHVFEGEGYPTPTDQRYCINSVSLKLVPSE"
     gene            1645..2538
                     /locus_tag="ND450_RS21395"
                     /old_locus_tag="ND450_21395"
     CDS             1645..2538
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="ND450_RS21395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ND450_21395"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_253320572.1"
                     /transl_table=11
                     /translation="MEPWGLSGPEFLQLYWVGLALAVLVSIVVRVRVRAGHSTQPVRSL
                     DMDELAYLAGGPRRVVETSIARLLTAGELRTSRRGTVQVTSSTQSLNAVDRAVITDSQR
                     YTNRTVNLMIPAVAKEGVVTGIGRRLEDMGLLVNPDVVKSAVRKGSIALWVLLAIGIAR
                     WVNGIAIGAAVGWLTLQLVLTGLLILLLTRRGKNVMRTGKGNDVLAAARTSNSSKARAG
                     TLDEVMYTGAAGAVLFGGLVVYPDLAVRSSLLASSGSGTYSSGTYSSCSSSSSSCSSGS
                     SCGGGGGCGGGCGGGS"
     gene            2628..3425
                     /locus_tag="ND450_RS21400"
                     /old_locus_tag="ND450_21400"
     CDS             2628..3425
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="ND450_RS21400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ND450_21400"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_253320573.1"
                     /transl_table=11
                     /translation="MSQSSAPHRATWSAEEIGFLSGGPGRAAEAVLARLLDGGLVRISR
                     EGLVTAVHQNGYGASSPLEAYVLAGLRGAARPINQVVQVAMASHEMGALHESLVQRGMV
                     QRQWGRPRGGKIALRVLLFLLAFVSVFVAFAIDTRLFLVTALLVFLAILLRKPGRLTRN
                     GKTMWLHALRNPRSSADSVALFGLRRGKQRMNTATSTSYIASSSCSSYDWSHHNCGGSS
                     SCSSSSCNSSSSCGSSSSCSSSSSSCSSSSCGSSSSSCGSSSS"
     gene            3437..4243
                     /locus_tag="ND450_RS21405"
                     /old_locus_tag="ND450_21405"
     CDS             3437..4243
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="ND450_RS21405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ND450_21405"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_253320574.1"
                     /transl_table=11
                     /translation="MGTPAAARGPWLSAEEAGYLAAGPGRAAETVLARLIDTGLVRVSR
                     DGLVSAVHQDGQDATTSIEARMLNHARTPVRFGAVILSATHSAEMTQLHRHLLDRNLMQ
                     RGRRRNEGWWFSLAVAGLLVLAGIPAPAFLFGVPIALGLFVWQYGRSPITQAGADALKH
                     VTAHDRVHAVALYGFSGTVGGQRVGALFDLPESVARMVPPSDERRSSPAVGRRPSPKGR
                     RTDWAGDAGGCGNTSSSCGSAGGCSGSSSSSCGGGSGCGGGGGGGD"
     gene            4243..5001
                     /locus_tag="ND450_RS21410"
                     /old_locus_tag="ND450_21410"
     CDS             4243..5001
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="ND450_RS21410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ND450_21410"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_253320575.1"
                     /transl_table=11
                     /translation="MATPATERGTRVPPERIGYLADGPGRAAETALARLLDAGLVRVSR
                     DGLVSAVHQGSYDTTTPVEVCILTGLSRPVRFDAVVRNAAKSKEMRDLHDRLRAEGLTR
                     RARPRLGVWWIFLVIGGVLALASLTEPWLLIGTVGFLGSAWWTYGAKPLTRVGRAALED
                     ASTSDRVIAVALEGFRGKVSGRPVGDLFDLPQSVVKTLPRKRKRRGTGGGTASGSAGCG
                     SGCGSSSCGSSCSSGGSSCGGGCGGGGGGD"
     gene            5001..5819
                     /locus_tag="ND450_RS21415"
                     /old_locus_tag="ND450_21415"
     CDS             5001..5819
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684640.1"
                     /locus_tag="ND450_RS21415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ND450_21415"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_253320576.1"
                     /sec_met_domain="DUF692 (E-value: 2.1e-85, bitscore: 285.2,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGIKDLGVGIGWRSEIDLTVERLPGVDFVEVVAENLHIGHLPES
                     VTLLLERGVPVLPHAVSLSLGGADPVDLHRVEHLGAVAEAVGAPMVSDHVCFVRAGGLD
                     SGHLMPVPRTRDALDVLVANVELAQANLPVPLALENVAALLEWPDGELSEGQFLAELVE
                     RTDCWLLVDVANLYANARNIGTDPERFLDEIPLERLAYVHVAGGEQHGDIYHDTHAHAV
                     LPEVLEVLDELCSRVAPPGVLLERDDNYPSDTEIASELAAIRAVVDKHRG"
     gene            5812..6183
                     /locus_tag="ND450_RS21420"
                     /old_locus_tag="ND450_21420"
     CDS             5812..6183
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671914.1"
                     /locus_tag="ND450_RS21420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ND450_21420"
                     /product="hypothetical protein"
                     /protein_id="WP_253320577.1"
                     /transl_table=11
                     /translation="MAEIVNENELLVARERLAEQQAELLKALLANGPAPAGFDEQRLGV
                     ERRALLSKRRGIVRMLGPAVADELGDRFRPLFDAYAVENPRRAGSRAREDAAAFAEWCR
                     ARGELPEKRRARWKFWQSN"
     misc_feature    complement(6326..6341)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.25"
                     /tool="antismash"
     gene            6360..9443
                     /locus_tag="ND450_RS21425"
                     /old_locus_tag="ND450_21425"
     CDS             6360..9443
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099298.1"
                     /locus_tag="ND450_RS21425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ND450_21425"
                     /product="S8 family serine peptidase"
                     /protein_id="WP_253320578.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 4.1e-62, bitscore:
                     209.2, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRGKTKVLGAVAALALTAAPAVATAAPADAPGKAVDEVTLVTGDK
                     VLLGHDRQVVAIQPGEGRQKMVFKTARTADGHTMVVPKDAFEPLAAGRLDRRLFDVTTL
                     LEFGYRDRMPLIVDGRARVGARSSNDWTALRSTAERGEKVWLDGIRKPSLDRSVAQIGA
                     PAAHQKGITGKGIKVAVVDTGVDEKHPDLVGQQIAEKNFTTDPDNTDNVGHGTHVGSTV
                     ASHDAKYGGVAPGAQLIDAKVCVEFGCQESWILDGMRWAAEQGAQVINMSLGGTDTPEV
                     DPLEQAVNDLTAQYGTLFVIAAGNSGAPGTVGSPGSADAALTVGAVDRDDKLADFSSRG
                     PRTGDSGLKPEVTAPGVEIVAAKAGSSDHVAFSGTSMATPHVAGAAALLRQQHPEWTPA
                     QLKSVLATTAKPTEGVSAFDQGAGRVNVAKAIDQVLTGDVTVNLGLHEWPHGDDKPVTK
                     PVTYSNPTDAAITLDLALKTTAPTGMFTLGAKKVTVPAKGTASVNVTGNTRAGTQDAAY
                     SGEVVATSGTHTVRTLVGIDREVESYTLTIDSIGRDGADPAESSSLLVGLDNSVFKPIG
                     GAGDLKVRLPKGRYFVETSLSNGERGFDLLYQPNVELTRDLTITQDARQAQPVSVKAPE
                     ETGDFTLGIISFRRSVDGQDRLGGTLFMFGGFDGLAIAQAGGPLPPADLSSIAMLQANT
                     TADPHTYYRLAYPVPGGFPVGLDKAPAKKDLARVESTIGGLKPDSLAQKGELPVTALGV
                     SSGTALYPVAVGATSVDYVSTEDVLWQSAVMTRHANGAQERITSSNRKYTAGKTYRERE
                     VRPVFSPGLPESPYGFVERYGTQLYVSPPMTSDGTHGTGSSTVDSARAALYRNGVLLGE
                     TTTPGGLFSDVPLPRGDFRAEVSLDRSSQFEFSTKVSAVWTFKSQGVEGRVESPPLSVV
                     RFEPKLDDNGAVPAGTLQRIGLLTQTQGDAGKVRITGVEYSHDDGRTWRRAGVVGNSAL
                     VYNPANAQWTSLKATATDARGGTVEVTVIRAYKVTSQP"
     misc_feature    complement(6405..6417)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.46"
                     /tool="antismash"
     gene            complement(9440..10132)
                     /gene="hemQ"
                     /locus_tag="ND450_RS21430"
                     /old_locus_tag="ND450_21430"
     CDS             complement(9440..10132)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983668.1"
                     /locus_tag="ND450_RS21430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ND450_21430"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_089923177.1"
                     /transl_table=11
                     /translation="MARLNYNELNDTIRYTMWSVFRVEPGKLPDDRSAAASETQNYLDA
                     LEGKGVVVRGVYDVAGLRADADFMIWWHAEEIEQVQQAYTGFRRTPLGKVSTPVWSQVA
                     LHRPAEFNRSHIPAFLAGEEARKYICVYPFVRSYEWYLLPEEDRRKMLADHGKEARDYP
                     DVRANTVASFALGDYEWLLAFEADELHRIVDLMRHLRGTEARLHVREEIPFYTGTRVPA
                     SELVLNLP"
     misc_feature    complement(10385..10398)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.86"
                     /tool="antismash"
     misc_feature    10531..10542
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
ORIGIN
        1 agtggtcgtc atcgcggtgc tcaagcgcct cggcgtggac gtcagcgcag tcgagggcct
       61 ggccaccgtg ccggtcctgg gccacggccg tgcggtcggc gccgtcaagc cgtcactcgc
      121 cctcgcaagc tgaacttgac gaccgacgtt gatccgacat tgacgcgtcc acctgcacat
      181 ggacgcgtca acatgtcgag ccggtcaacg ttgcggatca acacgtcaac ctccaagtca
      241 acgttgacgg tcacccccgc cgtcaacgcc cggcacacgg gaatccccca ccccagccac
      301 taggctgggg cttatgggcg attcgaagga cgagagcgag aagttccagg aggagctgat
      361 cggcctcgac ccgcacgacc ccgagacgaa ggcgttcgcc gaccacctgg accggatgca
      421 gcggcaacgc cccggttaca ccgtcgaggg ctacctgcag ggcgtgcagg acttcgccga
      481 ctcggcgaac cgcacgacgg gccaccagcg gctcgtcgcc gtcatcgtgg tggggctcat
      541 cctgctcggc gtgatcctga cgatctggtt cgcgctgggc gacatcatcg ccatcatctt
      601 ctgagccgcg gccgcgccga ccggcggctc tcggtccgcg cttggtctgc gtcgtgtggt
      661 ccgtgtcgtg tgaaccgcga catgagggca gcactgccac gccgtccccg ccgggcggat
      721 gtcgcgccac ctggcctccg ccccacccga cctccgctct cccgcttcac ccagcccagc
      781 ccggtccgcc ccaactcgcc ggcctcagcc cacccgcctc agccgcccgc cccacaaccc
      841 acacccggcc ctcgcacccc agcccttcca ggccaaacgg gcccgactcc ttaacggggt
      901 cttacgaccg cccccgagga tcccccgccc gggaagctcc acagcccccg cggagttata
      961 aagagacatg gaacctgttg tcggcgccac cccgaaagtc gtgcgttccg aagaggaatg
     1021 gcgcgcccag ctcaaccccg ccgagtacgc ggtgctgcgg caggcaggca ccgagcgtcc
     1081 gtggaccggc gagtacaccg agaccaagac cgagggcctc tacgagtgcc gtgcgtgtgg
     1141 agcggagctg ttccgcagcg acaccaagtt cgactcgcat tgcgggtggc cgtcgttctt
     1201 ctcgccgttg gccggtgact cggtcatcct gcgcgaggac cgcacgctcg gcatgaagcg
     1261 cgtggaggtg ctctgcgcca cgtgccacag ccacctcggg cacgtcttcg agggcgaggg
     1321 ctaccccact ccgacggacc agcgctactg catcaactcc gtctccttga aactcgttcc
     1381 gtccgaatag gagactgggc tcggtccggt cagcagcgat cggacggacc tgggaggcat
     1441 catgatcttc ttcggcaccg acggtgaagg ctccagcgga gtcgacggcg gttcgtcgag
     1501 ctagcccgcc gagagccaca accggggaac acaggcagaa ggccggcaac cctaaggctt
     1561 cgtcaacaag ccttgtgggc ctgtggcgcg ggccacacca ttgacgcacc gaagcacttt
     1621 cggggcagtc agggggtggg tcgcatggag ccttgggggt tgtccgggcc ggagttcctt
     1681 cagctgtact gggtcgggct ggcgctggcc gtgctggtct cgatcgtcgt tcgggtacgc
     1741 gtgcgggcgg gtcacagcac ccagcccgtg cgctccctgg acatggacga gctcgcctat
     1801 cttgcgggcg gtccaaggcg cgtggtcgaa acgtccatcg cgcggctgct caccgcgggt
     1861 gagctcagga cttcacggcg tggcaccgtt caggtcacgt ccagcaccca gtcgctcaac
     1921 gcggtggacc gcgccgtcat caccgacagc cagcgctaca ccaaccgcac cgtcaacctc
     1981 atgatcccgg ccgtcgccaa ggaaggcgtg gtcacgggca tcggccggcg gctcgaggac
     2041 atgggcctgc tggtcaaccc ggacgtcgtg aaaagcgcgg tgcgcaaggg atccatcgcg
     2101 ctgtgggtgt tgctcgcgat cgggatcgcg cgctgggtca acggcatcgc gatcggtgcc
     2161 gcggtcggct ggctcaccct ccagctcgtc ctcaccggcc ttctcatcct gctgctgacg
     2221 cgccgcggca agaacgtcat gcgcaccggc aagggcaacg acgtgctcgc ggccgcccgc
     2281 accagcaaca gctccaaggc gcgggcggga accctggacg aggtcatgta cacgggtgcg
     2341 gccggcgcgg tgctgttcgg cgggctcgtc gtctacccgg acctggcggt gcgcagttcg
     2401 ctgctcgcct cgtcgggatc cggcacgtac agcagcggta cttacagctc gtgttcgtcc
     2461 agttcttctt cgtgttcgag cggaagcagt tgtggcggtg gcggcggctg cggtggtggc
     2521 tgcggcggcg gcagctgatc cggtgttcgg tccttttccg gtaacgacag gaacccaggg
     2581 gtagatctca cgtcgaagat gcactgacac cagtgagggg agtgctgatg tcccagtctt
     2641 cggcaccgca ccgggccacc tggtccgctg aggagatcgg gttcctgtca gggggaccgg
     2701 gcagggcggc ggaggccgtc ctcgcccggc tgctggacgg cggtctcgtg cgcatctccc
     2761 gcgagggtct cgtgaccgcc gtccaccaga acggttacgg cgcgtcgagt ccgctggagg
     2821 cgtacgtcct cgccggactc cgcggcgccg cgcgtccgat caaccaggtc gtgcaggtgg
     2881 cgatggcgag ccacgagatg ggcgcgttgc acgagagcct cgtgcaacgc ggaatggtgc
     2941 aacgccagtg gggccggcct cgcggcggca agatcgcgtt gcgggtgctg ctgttcctgc
     3001 tggcgttcgt ctcggtcttc gtcgcgttcg cgatcgacac cagactgttc ctggtgacgg
     3061 ctctgctggt gttcctggcg atcctgttgc ggaaaccggg caggctcacc aggaacggca
     3121 agaccatgtg gctgcacgcg ctgaggaatc cgcgcagcag tgccgactcg gtggcgctgt
     3181 tcgggttgcg caggggcaaa cagcgcatga acaccgcaac gtccacttcg tacatcgcga
     3241 gcagcagctg cagctcctac gactggagcc accacaactg cggcggctcg tcgagctgtt
     3301 ccagcagcag ctgcaacagt tcgagcagtt gcggtagttc aagcagttgt tcgagcagta
     3361 gcagcagctg cagcagctcg agttgcggca gcagtagcag cagctgcgga agttccagca
     3421 gctgaggggg aagcagatgg gaacaccggc agcggcgcgg gggccgtggc tgtccgcgga
     3481 ggaggccggc tacctggcgg cgggtcccgg acgggcggcg gagacggtgc tggcccggtt
     3541 gatcgacacc ggcctggtgc gggtgtcccg cgacggcctc gtctccgccg tgcaccagga
     3601 cggtcaggac gccaccacgt cgatcgaggc gcggatgctg aaccacgcgc ggacacccgt
     3661 gcgcttcggc gcggtcatcc tgtcggcgac gcacagcgcc gagatgacgc agctgcaccg
     3721 gcacctgctc gaccggaacc tgatgcaacg gggccgccgt cgcaacgagg gctggtggtt
     3781 ctccctcgcg gtcgcgggcc tgctggtcct ggcgggcatc ccggcaccgg cgttcctctt
     3841 cggcgtgccg atcgcactcg ggttgttcgt ctggcagtac ggccgcagcc cgatcaccca
     3901 agcgggcgcg gacgcgctga agcacgtgac cgcgcacgac agggtgcacg cggtggcgct
     3961 gtacgggttc tccggcacgg tgggcggcca acgcgtcggc gcgctgttcg acctccccga
     4021 gtccgtggcg agaatggtcc cgcccagcga cgagcggcgg tcgtcgcccg cggtggggcg
     4081 gcggccgtcc ccgaaaggcc gccggactga ctgggcgggc gacgccggcg ggtgtggcaa
     4141 cacgtccagc agttgcggca gcgcaggcgg ttgtagtggt tcgagcagtt ccagttgcgg
     4201 cggcgggagc ggctgcggcg gcggtggagg cggaggcgac tgatggcgac accggcgacg
     4261 gaaaggggaa cgcgggttcc cccggagcgg atcggctacc tggcggacgg gcccggcagg
     4321 gcggcggaga cggcgctggc caggctgctc gacgccggcc tggtccgggt ctcgcgcgac
     4381 gggctggtct cggccgtgca ccagggctcc tacgacacca ccacgccggt cgaggtgtgc
     4441 atcctcaccg gcctgagcag gccggtgagg ttcgacgcgg tcgtgcgcaa cgccgcgaag
     4501 agcaaggaga tgcgtgatct gcacgaccgg ctgcgcgccg aggggctgac ccgcagggcc
     4561 cgtccccggc tcggggtgtg gtggatcttc ctcgtgatcg ggggtgtcct cgccctcgcc
     4621 tcgctgacgg aaccgtggct gctcatcggc acggtgggct tcctggggtc cgcctggtgg
     4681 acgtacggcg cgaaaccgct caccagggtg ggcagggcgg cgctggagga cgcgagcacg
     4741 agcgaccgcg tcatcgcggt ggccctggag ggcttccgcg gcaaggtcag cggccggccg
     4801 gtcggtgacc tcttcgacct gccgcagagc gtcgtcaaga ccctgccccg caagaggaag
     4861 agacggggca cgggcggcgg caccgcgtcg ggcagcgcgg gctgtggcag cgggtgtggc
     4921 agcagcagct gtggtagcag ttgttcgtcg ggcggcagta gctgcggcgg cggttgtggc
     4981 ggcggcggag gaggcgactg atggcgggga tcaaggactt gggcgtcggc atcggctggc
     5041 gctcggagat cgacctgacc gtggaacggc tgcccggcgt ggacttcgtc gaggtcgtcg
     5101 ccgagaacct ccacatcgga cacctgccgg agtcggtgac tttgctgctg gaacgcggcg
     5161 tcccggtgct cccccacgcc gtgtcgctgt cgctgggcgg cgcggacccc gtcgacctgc
     5221 accgcgtcga gcacctgggc gcggtcgccg aggcggtcgg cgcgccgatg gtcagcgacc
     5281 acgtgtgctt cgtgcgggcg ggcggcctcg actcgggcca cctgatgccg gtgccgcgca
     5341 ccagggacgc gctcgacgtg ctcgtcgcga acgtcgagct ggcccaggcg aacctgcctg
     5401 tcccactggc gctggagaac gtcgccgctc tgctggagtg gccggacggc gaactgtccg
     5461 agggccagtt cctcgccgaa ctcgtcgagc gcacggactg ctggctgctc gtggacgtcg
     5521 cgaacctcta cgcgaacgcc cgcaacatcg gcacggaccc cgagcggttc ctggacgaga
     5581 tcccgctgga acgcctggcg tacgtgcacg tggccggcgg cgagcagcac ggcgacatct
     5641 accacgacac gcacgcccac gcggttctgc ccgaagtgct ggaagtgctc gacgaactgt
     5701 gctcccgcgt cgcgccgccc ggagttctgc tggaacgcga cgacaactac ccgtccgaca
     5761 ccgaaatcgc gagcgaactg gccgcgatcc gcgccgtcgt ggacaagcac cgtggctgaa
     5821 atcgtgaacg agaatgaact tttggttgca cgcgaaaggc ttgccgagca gcaggcggag
     5881 ctgctaaaag cattgctcgc gaacggaccg gcaccggcgg gattcgacga acaacgcctc
     5941 ggtgtcgaac gccgcgcttt gttgtcgaaa cggcgcggca tcgtgcggat gctgggtccg
     6001 gcggtcgcgg acgagctcgg cgaccggttc cggccgctct tcgacgcgta cgccgtcgaa
     6061 aacccacgtc gtgcgggctc tcgggcccgt gaggacgccg cggcgttcgc ggaatggtgc
     6121 cgcgctcgcg gagaactgcc ggagaagcgc agggcacgct ggaagttctg gcagtcgaac
     6181 tgacgggcga gtgatccgag ggccagaatc ccccgttcgg ccctgtgtga cccagatcgc
     6241 catcgatacg ttcgaaaggg tcggggggca ctttttccat tgcgtcaaca ttgccgcaaa
     6301 ggccttccgg cctgcgcgga ccacagcgcg cagaaattcg aaccgtcaag gggattcgaa
     6361 tgcgcggaaa aacgaaagtg ctgggcgccg tcgcggcgct cgccctgaca gcggcaccgg
     6421 ccgtcgccac ggcggcaccg gcggacgcac ccggcaaggc cgtcgacgag gtcacgctcg
     6481 tcacggggga caaggtcctg ctgggccacg accggcaggt cgtcgcgatc cagcccggcg
     6541 agggccgcca gaagatggtc ttcaagaccg cccgcaccgc ggacggtcac acgatggtgg
     6601 tgccgaagga cgccttcgag cccctggcgg cgggccggct cgaccgcagg ctcttcgacg
     6661 tcaccacgct gctggagttc ggctaccgcg accgcatgcc gttgatcgtg gacggccggg
     6721 cgagagtcgg cgcccggtcg tcgaacgact ggacggcgct gcgctccacg gcggagcggg
     6781 gcgagaaggt ctggctcgac ggcatccgca agccgagcct cgaccgcagc gtcgcccaga
     6841 tcggcgcgcc ggccgcccac cagaagggca tcaccggcaa ggggatcaag gtcgccgtcg
     6901 tcgacacggg cgtcgacgag aagcaccccg acctcgtggg tcagcagatc gcggagaaga
     6961 acttcacgac cgaccccgac aacaccgaca acgtcggcca cggcacgcac gtcgggtcga
     7021 ccgtcgcgag ccacgacgcg aagtacggcg gtgtcgcgcc gggcgcgcaa ctgatcgacg
     7081 cgaaggtctg cgtcgaattc ggctgccagg agtcctggat cctcgacggc atgcgctggg
     7141 ccgccgagca gggcgcccag gtgatcaaca tgagcctcgg cggcaccgac accccggagg
     7201 tcgacccgct ggagcaggcg gtcaacgacc tgacggccca gtacggcacg ttgttcgtca
     7261 tcgcggcggg caactcgggt gcaccgggca cggtgggctc accgggttcg gccgatgcgg
     7321 ccctgacggt cggcgccgtc gaccgcgacg acaagctcgc cgacttctcc agcagaggcc
     7381 ctcgcaccgg agacagcggc ctcaagccgg aggtgaccgc accgggtgtc gagatcgtcg
     7441 ccgccaaggc cggttcctcc gaccacgtcg cgttcagcgg cacgtcgatg gcgaccccgc
     7501 acgtggcggg cgcggcggcg ttgctcaggc agcagcaccc ggagtggacg cccgcgcagc
     7561 tcaagtcggt gctcgcgacc acggccaagc cgaccgaggg cgtcagcgcg ttcgaccagg
     7621 gcgcgggccg cgtcaacgtc gccaaggcga tcgaccaggt gctgaccggc gacgtgaccg
     7681 tcaacctcgg cctgcacgag tggccgcacg gcgacgacaa gccggtgacc aagcccgtca
     7741 cctactccaa cccgacggac gcggccatca cgctcgacct ggcgctcaag accaccgcgc
     7801 cgaccggcat gttcaccctg ggcgccaaga aggtcaccgt gccggccaag ggcaccgcgt
     7861 cggtcaacgt caccgggaac acccgcgccg gcacccagga cgccgcgtac tcgggcgagg
     7921 tcgtcgccac gtccggcacg cacaccgtcc gcacgctcgt cggcatcgac cgcgaggtcg
     7981 agagctacac gctcacgatc gactcgatcg gccgtgacgg cgctgacccg gccgagtcgt
     8041 cctcgctcct cgtcggcctg gacaacagcg tgttcaagcc catcggtggc gcgggggacc
     8101 tgaaggtgcg cctgcccaag ggcaggtact tcgtcgagac ctccctgtcc aacggcgaac
     8161 gcggcttcga cctgctgtac cagccgaacg tggaactcac ccgcgacctg acgatcacgc
     8221 aggacgcccg ccaggcccag ccggtgtcgg tgaaggcgcc ggaggagacc ggtgacttca
     8281 cgctgggcat catcagcttc cgccgctcgg tggacggaca ggaccgcctg ggaggcacgc
     8341 tgttcatgtt cggcggcttc gacggtctcg cgatcgccca ggccggcggg ccgctgccac
     8401 ccgccgacct gtcctcgatc gcgatgctcc aggccaacac cacggccgac ccgcacacgt
     8461 actaccggct cgcctacccg gttcccggcg gcttccccgt cggcctggac aaggcgccgg
     8521 ccaagaagga cctggccagg gtcgagtcca cgatcggggg cctgaagccg gacagcctcg
     8581 cccagaaggg cgagctcccg gtcaccgcgc tcggggtgag cagcggcacc gcgctctacc
     8641 cggtggccgt gggcgccacc tccgtggact acgtgtcgac cgaggacgtg ctctggcagt
     8701 ccgcggtgat gacgcggcac gccaacggcg cgcaggaacg catcacctcg tcgaaccgca
     8761 agtacacggc gggcaagacc tatcgggaga gggaagtccg gccggtgttc tcgccgggtc
     8821 tgcccgagtc gccgtacggt ttcgtcgagc ggtacggcac gcagctctac gtcagcccgc
     8881 cgatgacgag cgacggcacg cacggcaccg gctcgtccac tgtggacagc gccagggccg
     8941 ccctgtaccg caacggtgtg ctgctcgggg agaccaccac ccccggcggc ctgttctcgg
     9001 acgtgccgct gccgcggggt gacttccgcg ccgaggtgag cctcgaccgg agctcgcagt
     9061 tcgagttcag caccaaggtg tccgcggtgt ggacgttcaa gtcccagggc gtcgagggcc
     9121 gggtcgagtc gccgccgctg tcggtggtgc gcttcgagcc gaagctcgac gacaacggcg
     9181 cggtgcccgc gggcacgttg cagcgcatcg ggttgctcac gcagacgcag ggcgacgcgg
     9241 gcaaggtgcg catcaccggt gtcgagtact cgcacgacga cggcaggacg tggcgccgtg
     9301 cgggcgtcgt cggcaacagc gcgctggtct acaacccggc gaacgcgcag tggacttcac
     9361 tgaaggccac tgcgacggac gccaggggtg gaaccgttga ggtgaccgtg atccgggctt
     9421 acaaggtcac ctctcagccc taaggcaggt tgagcacgag ttcggacgcc gggacgcggg
     9481 tgcccgtgta gaaggggatc tcctctcgca cgtgcagccg cgcctcggtg ccgcgcaggt
     9541 gccgcatgag gtcgacgatg cggtgcagct cgtcggcctc gaacgcgagc agccactcgt
     9601 agtcgccgag ggcgaacgac gcgacggtgt tcgcgcgcac gtccgggtag tcgcgggcct
     9661 ccttgccgtg gtccgccagc atcttgcggc ggtcctcctc cggcagcagg taccactcgt
     9721 aggaccgcac gaacgggtag acgcagatgt acttgcgcgc ctcctcgccc gcgaggaacg
     9781 cggggatgtg gctgcggttg aactccgcgg ggcggtgcag ggcgacctgc gaccagaccg
     9841 gcgtggagac cttgccgagc ggcgtgcggc ggaagcccgt gtaggcctgc tgcacctgct
     9901 cgatctcctc ggcgtgccac cagatcatga agtcggcgtc ggcgcgcagg ccggcgacgt
     9961 cgtacacgcc gcggacgacg acgcccttgc cctccaacgc gtccaggtag ttctgggtct
    10021 cgctggcggc cgccgagcgg tcgtccggga gcttgccggg ttcgacccgg aacaccgacc
    10081 acatcgtgta gcggatggtg tcgttgagct cgttgtagtt caagcgcgcc atacgtccat
    10141 cttggcacgc gcccctcaac ggcgaacgcg gcccagcagg tgtgctgcga cacgggcggc
    10201 ggccgcgtcg ccggacgcga tgcaggccgg cacgcccacg ccgtgcagcg tggcgcccgc
    10261 cacggagagg cccggcacgt ccgcgatgcc gcgttcgatc gtctccacca ggtcgacgtg
    10321 accgacgccg tactgcggca gcccgccgcc ccagcgctgc accacggagt cgatcggcga
    10381 ggccttgatg cccgtcaact cgtgcaggtc gttgcgcacg gccttgacca ggtcggcgtc
    10441 gctgcgctgc agcagcgccg tctcgccgtg ccgcccgacc gagccgcgca ggatcacggg
    10501 gtcgccgtag tgcgaccact tgcggctgga gaacgtgaac gccttggcgg tgaacggggt
    10561 gatgcggtcg gagtaccgct ccccctccgc gatcaggatg ccggagcgct gcggcagttc
    10621 cgtgcccggc ggcagcgcca gcgcgacgac ggccatcgag gcgacctcga ccttgccgta
    10681 cgcggcggac acctgcgggg cgatctcgtg cagcagcttg cgcgcggacg gcgccgggac
    10741 cgcgagcacg acgccgtcgc actccaggta ctccggggtc gcggccgagc cgatctccag
    10801 gcgccagccg tggccgagc
//
