LOCUS       NZ_CP099468            10522 bp    DNA     linear   CON 04-JUL-2024
DEFINITION  Streptomyces phaeoluteigriseus strain Qhu-M197 chromosome, complete
            genome.
ACCESSION   NZ_CP099468
VERSION     NZ_CP099468.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces phaeoluteigriseus
  ORGANISM  Streptomyces phaeoluteigriseus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group.
REFERENCE   1  (bases 1 to 9026270)
  AUTHORS   Zhang,B., Xiang,X. and Fan,J.
  TITLE     Complete genome sequence of soil microorganisms Streptomyces sp.
            Qhu-M197 isolated from Alpine meadows habitats on the Tibetan
            Plateau
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9026270)
  AUTHORS   Zhang,B., Xiang,X. and Fan,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2022) Faculty of Eco-Environmental Engineering,
            qinghaiuniversity, No. 251 Ningda Road, Chengbei District, Xining
            City, Qinghai Province 810000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Hifiasm v. v0.14; Circlator v. v1.5.5; Pilon v.
            v1.22
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 68.51291x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023923245.1-RS_2024_07_04
            Annotation Date                   :: 07/04/2024 06:06:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,100
            CDSs (total)                      :: 8,006
            Genes (coding)                    :: 7,707
            CDSs (with protein)               :: 7,707
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 299
            CDSs (without protein)            :: 299
            Pseudo Genes (ambiguous residues) :: 0 of 299
            Pseudo Genes (frameshifted)       :: 130 of 299
            Pseudo Genes (incomplete)         :: 241 of 299
            Pseudo Genes (internal stop)      :: 34 of 299
            Pseudo Genes (multiple problems)  :: 90 of 299
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:40:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7778032
            Orig. end                         :: 7788554
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP099468.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10522
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="33"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10522
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10522
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5522](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5522)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    complement(78..96)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 19.31"
                     /tool="antismash"
     misc_feature    complement(89..103)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.26"
                     /tool="antismash"
     misc_feature    complement(120..134)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.53"
                     /tool="antismash"
     misc_feature    complement(121..134)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.3"
                     /tool="antismash"
     gene            222..1997
                     /locus_tag="NFX46_RS34390"
                     /old_locus_tag="NFX46_34390"
     CDS             222..1997
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020132945.1"
                     /locus_tag="NFX46_RS34390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_34390"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_252554687.1"
                     /transl_table=11
                     /translation="MRSVLRALRRLAPASTRDISRATGLPVPIVAALGNELRRRDLLTK
                     ERPSRLTPAGLDVVAELGMDLTLDATCTTCEGRELVIPDVLEEAVRRLSDLMESGPAAD
                     MSIDQSHCTPETKVRRVLALLAAGALPGGNLLLIGDDDLVSLAVAVVGDVLGSPIVERV
                     TVVDISPEILAYIRKVSAGLGTRVETVEHDLRHPLPTELHSGFDVAMTDPPYTAEGARL
                     FLSRAVEGLRPGPSHSVFFSFGGKSPDEMLEVQREIMALGLVTNGYIRNFNEYQGSGIL
                     GGVGFFQHLLTTTSTASHQGDFEGPLYTGDKRTRQREYTCVACNTALRVGPGARWTSVA
                     ALRDEGCPQCGKGPFRPGRLVAADEPPTTLAVTVDEPAPAPAPAPAPVPVSEAAPAPAP
                     ITTPAPVPALAQPSHSLRPGWQRPTPESRAALEQRARPCLTRQADEGDLPAIGEFEAEI
                     ARISFGEDAVDDPARHASRVGRSMEKSKEGMIVAHRPDGETVGWCWVSINQNQMTGDRY
                     ANFRSLAVAPLDNRADVAELLLTEGLEFCLANGITEVVGRVHVGNVPMRTVYRKFGFDP
                     ATLTMKLRLPEEESR"
     gene            1994..3202
                     /locus_tag="NFX46_RS34395"
                     /old_locus_tag="NFX46_34395"
     CDS             1994..3202
                     /NRPS_PKS="Domain: FkbH (45-165). E-value: 2.7e-17. Score:
                     54.9. Matches aSDomain:
                     nrpspksdomains_NFX46_RS34395_FkbH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1256: FkbH like protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020132944.1"
                     /locus_tag="NFX46_RS34395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_34395"
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="WP_289782114.1"
                     /transl_table=11
                     /translation="MTGATGATGTTDATGAPDPTGATGASSATGASSAPDPTGYDADRI
                     KCVVWDLDGTLWDEIAVETPTDELPVPRPEVLAAIDALAARGVLSSLASRSAPTVLDRM
                     DAVPGLRERFLAPQVSWQDKSESLRRIAKDLGIAVEALVLIDDSPYERAEVEALLPGVR
                     TLDPGEVPALLAAFEDREVTPESRERVRRYRTEETRRAEGERFQGTREEFLRWCDMRLT
                     VGPAVAEEVPRALELAARTHRLNSSGLTPDRLRALAAADGHELYTARMSDRFGEYGIIG
                     AALVDRTARGSWSVPLLALSCRVAGRGAAAAFLFWLMERARESGAEDFRVTLRPTDANL
                     EMRILLRQAGLRRTEADDSADGSTAGDDGAVLARTLDSGGGGGGLPKPPAWLHVFTRKD
                     ADA"
     aSDomain        2129..2488
                     /aSDomain="FkbH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NFX46_RS34395_FkbH.1"
                     /evalue="2.70E-17"
                     /label="NFX46_RS34395_FkbH.1"
                     /locus_tag="NFX46_RS34395"
                     /protein_end="165"
                     /protein_start="45"
                     /score="54.9"
                     /tool="antismash"
                     /translation="KCVVWDLDGTLWDEIAVETPTDELPVPRPEVLAAIDALAARGVLS
                     SLASRSAPTVLDRMDAVPGLRERFLAPQVSWQDKSESLRRIAKDLGIAVEALVLIDDSP
                     YERAEVEALLPGVRTL"
     gene            3199..3501
                     /locus_tag="NFX46_RS34400"
                     /old_locus_tag="NFX46_34400"
     CDS             3199..3501
                     /NRPS_PKS="Domain: PP-binding (20-92). E-value: 3e-10.
                     Score: 32.3. Matches aSDomain:
                     nrpspksdomains_NFX46_RS34400_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020132943.1"
                     /locus_tag="NFX46_RS34400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_34400"
                     /product="phosphopantetheine-binding protein"
                     /protein_id="WP_252554688.1"
                     /sec_met_domain="PP-binding (E-value: 1.5e-06, bitscore:
                     27.4, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDAHTPSSTSSADRAAVEEELRAMIAEAARLDAAVVAGLPADTD
                     LFGPEIGLTSLAGVTLLGAVDARFGVDVAALDLSLDSLQSIATLADFVTAHRSAH"
     aSDomain        3259..3474
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NFX46_RS34400_PP-binding.1"
                     /evalue="3.00E-10"
                     /label="NFX46_RS34400_PP-binding.1"
                     /locus_tag="NFX46_RS34400"
                     /protein_end="92"
                     /protein_start="20"
                     /score="32.3"
                     /tool="antismash"
                     /translation="ELRAMIAEAARLDAAVVAGLPADTDLFGPEIGLTSLAGVTLLGAV
                     DARFGVDVAALDLSLDSLQSIATLADF"
     gene            3638..3961
                     /locus_tag="NFX46_RS34405"
                     /old_locus_tag="NFX46_34405"
     CDS             3638..3961
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106099.1"
                     /locus_tag="NFX46_RS34405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_34405"
                     /product="chaplin"
                     /protein_id="WP_252554689.1"
                     /transl_table=11
                     /translation="MSRIAKAAAVAVGAGAMMVSGAGLAMADADAQGAAVGSPGVLSGN
                     VVQVPIHVPINICGNTVNIVGLLNPAFGNTCANVSNHDKGEDKGEDKGGQHHSGGDASG
                     YGG"
     gene            complement(4151..5017)
                     /locus_tag="NFX46_RS34410"
                     /old_locus_tag="NFX46_34410"
     CDS             complement(4151..5017)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="NFX46_RS34410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_34410"
                     /product="tyrosinase family protein"
                     /protein_id="WP_252554690.1"
                     /transl_table=11
                     /translation="MAYIRKDVSTLTTGERRRFVRALLELKRRGEYDEFVRMHIDYYTS
                     DGERGLRTAHMTPSFLPWHRRFLLDLENALRRVDPSVTVPYWDWTRDRAATSVPWTADL
                     LGGNGRRSDRQVMTGPFAHATGNWTIRDGVTDTKFLTRDLGRARDPIGLPTKADVEWAL
                     SDPVYDVSPWDSTVTRGFRNKLEGWGTGRGSTAWLNHNRVHRWVGGAMVGGASVNDPVF
                     WLHHAFIDLLWTRWQRRHRTHRYLPAEPPLPDSSQFRRVVARHQRLPPWNVTPDQLEDA
                     GRIYRYA"
     gene            complement(5001..5522)
                     /locus_tag="NFX46_RS34415"
                     /old_locus_tag="NFX46_34415"
     CDS             complement(5001..5522)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019065674.1"
                     /locus_tag="NFX46_RS34415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_34415"
                     /product="tyrosinase cofactor"
                     /protein_id="WP_252554691.1"
                     /sec_met_domain="melC (E-value: 7.9e-43, bitscore: 144.8,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVSVDGVAAVTTGPARKARRGTRRSALRGLLGATAALALAPLVA
                     ASRPARRQKGQGHGHGYGHAPAPALGLGLGVNAFDETYLGRHLLGRLVPPVGVADTGRW
                     EITVDGRPLHLMRRADGTWLSMVDHYRSYADPLDAVRAAVDELGPGQMLRPAAATGRHT
                     HMGGDHGLHP"
     misc_feature    5539..5551
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.46"
                     /tool="antismash"
     gene            complement(5663..6181)
                     /locus_tag="NFX46_RS34420"
                     /old_locus_tag="NFX46_34420"
     CDS             complement(5663..6181)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976098.1"
                     /locus_tag="NFX46_RS34420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_34420"
                     /product="hypothetical protein"
                     /protein_id="WP_252554692.1"
                     /transl_table=11
                     /translation="MKSTTRGTLAAVLTCVAAAAGAVSATSAAAVGTVPVPVPLDGVEK
                     SLHVELPKIGGEIPVPVPGAPEGPRYVEGRLIPERVIPQLPVAAGLPGLGMRSPLPEVL
                     GDGFNHVGLDVPASDVRTLGPGLALDAPLTAPNADRFGLPDLQLPQAGVLTPVVQTVPG
                     ADLGVGQGL"
     misc_feature    6244..6255
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.12"
                     /tool="antismash"
     gene            complement(6910..7407)
                     /locus_tag="NFX46_RS34425"
                     /old_locus_tag="NFX46_34425"
     CDS             complement(6910..7407)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116484.1"
                     /locus_tag="NFX46_RS34425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_34425"
                     /product="DUF5949 family protein"
                     /protein_id="WP_252554693.1"
                     /transl_table=11
                     /translation="MTTTPGATRPFPVADLGTLVMMPWSGEAADGRAMPYLLACSLGNG
                     PGGHEGATAAVERLLEFSGLVVGGITDGTQKLSLPVTMLVVAGQAVVTMPWLRTQCTVP
                     PEWLEAVAERGYAYLLFTTRPWPGDAPGSTVAAEALNTFAGDEETLKAAAHIILPARSL
                     RA"
     gene            complement(7585..7866)
                     /locus_tag="NFX46_RS34430"
                     /old_locus_tag="NFX46_34430"
     CDS             complement(7585..7866)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445910.1"
                     /locus_tag="NFX46_RS34430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_34430"
                     /product="hypothetical protein"
                     /protein_id="WP_073490794.1"
                     /transl_table=11
                     /translation="MKSLKAAAVVAGSLVIAGAAAPAFAHDTADLTPTSLNGAVNTLTK
                     GPIDVMPLQHQSDTLDTENKNSPLSGVKDATTALNAPKSLLGGLPLQG"
     gene            complement(8036..8812)
                     /locus_tag="NFX46_RS34435"
                     /old_locus_tag="NFX46_34435"
     CDS             complement(8036..8812)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NFX46_RS34435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="NFX46_34435"
                     /product="hypothetical protein"
                     /protein_id="WP_252554694.1"
                     /transl_table=11
                     /translation="MRGLPVRRTAFTALCVTLLLGIAAPSALAADGEATRERTHAVPSA
                     SLSGADALLARARSLDDVKPALAPVTDLLDTSLRADSQGRSADRAKRLGAAAEDALTRV
                     AESTGPAATPSHGDRVGPGARARAADITDDLLATVERQLDALLEAATSGDVGQILPAAT
                     SLVTGLSDLVTSLVDGLSLPTLPTLPTLPTLPTLPTLTGLTGLTGLEGLGDLPEVSTLP
                     AETPALPVTTPALPVTPSTLPAQVPPLTPKGQAPAA"
     misc_feature    complement(8824..8837)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.21"
                     /tool="antismash"
     misc_feature    complement(8853..8867)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.99"
                     /tool="antismash"
     gene            complement(9120..9359)
                     /locus_tag="NFX46_RS34440"
                     /old_locus_tag="NFX46_34440"
     CDS             complement(9120..9359)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056482.1"
                     /locus_tag="NFX46_RS34440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_34440"
                     /product="chaplin"
                     /protein_id="WP_073490790.1"
                     /transl_table=11
                     /translation="MSRIAKGLVLTSVAAAAVAGASGIAAADSGAKAVAANSPGVLSGN
                     VIQVPIHIPVNVCGNTVNVIGLLNPAFGNTCIND"
     misc_feature    complement(9459..9474)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.86"
                     /tool="antismash"
     misc_feature    9464..9476
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 20.33"
                     /tool="antismash"
     misc_feature    complement(9518..9538)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 21.09"
                     /tool="antismash"
     misc_feature    complement(9578..9592)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.17"
                     /tool="antismash"
ORIGIN
        1 aacagcgttc cccatttctc ctgttccttt cctaattcac cgatccgcgg gtgtgtccgg
       61 ccgtcgtcgc gccagcccgt atactcgata gacatgtatt ccatcagccc gcccggagca
      121 gctttgtgaa aaacgcgcct cgacaccgtc ggagtacccc cgccgatcga gtgctcacag
      181 aagtcgcgga ggccgtcagg cttcaggagg gcccgcccgg agtgcgttcc gtgctgcgtg
      241 ccctgcgtcg tctggccccc gcctcgacca gggacatcag ccgggccacc ggcctgcccg
      301 tcccgatcgt ggccgccctc gggaacgagc tgcgccgacg cgacctcctc accaaggagc
      361 gtccctcccg gctgaccccg gccggcctgg acgtcgtggc cgagctgggc atggacctca
      421 cgctggatgc cacctgcacc acctgcgagg gccgcgaact ggtcatcccc gacgtgctgg
      481 aggaggcggt acggcggctc agcgacctga tggagtcggg ccctgccgcc gacatgtcga
      541 tcgaccagtc ccactgcacg cccgagacca aggtgcgccg cgtcctggcc ctgctcgcgg
      601 cgggcgcgct gccgggcggc aaccttctgc tgatcggcga cgacgacctc gtctccctgg
      661 ccgtcgccgt cgtcggcgac gtgctcggct ccccgatcgt cgagcgggtg accgtcgtcg
      721 acatctcccc cgagatcctc gcctacatcc ggaaggtgtc ggccggtctc ggcacccgcg
      781 tcgagacggt cgagcacgac ctgcgccacc ccctgcccac cgagctgcac agcggtttcg
      841 acgtggcgat gaccgacccg ccctacaccg cggaaggcgc ccgcctgttc ctctcgcgtg
      901 ccgtggaggg cctgcgcccc ggcccctcgc acagcgtctt cttctccttc ggcggcaaga
      961 gcccggacga gatgctcgag gtccagcgcg agatcatggc gctcgggctg gtcaccaacg
     1021 gctacatccg caacttcaac gaataccagg gcagcggcat cctcggcggg gtcggcttct
     1081 tccagcacct cctcaccacc acctcgaccg cctcccacca gggcgacttc gaaggccccc
     1141 tctacaccgg cgacaagcgg acccgccagc gcgagtacac gtgcgtggcc tgcaacaccg
     1201 cgctgcgcgt cggccccggc gcccgctgga cgtccgtcgc cgccctgcgt gacgagggct
     1261 gcccccagtg cggcaagggc cccttccgcc ccggccgcct ggtcgccgcc gacgagcccc
     1321 cgaccaccct cgcggtcacg gtggacgagc cggcccccgc accggcaccg gcaccggcac
     1381 ccgtacccgt atcggaagct gcaccggcac cggcacccat caccaccccc gccccggtcc
     1441 cggccctcgc gcagccctcc cactccctgc gccccggctg gcagcgcccc acccccgagt
     1501 cccgcgccgc cctcgaacag cgggcgcggc cctgcctcac ccgtcaggcc gacgagggcg
     1561 acctcccggc catcggggag ttcgaggccg agatcgcccg catctccttc ggcgaggacg
     1621 ccgtcgacga ccccgcccgg cacgcgtccc gcgtcggccg atccatggag aagtcgaagg
     1681 agggcatgat cgtcgcccac cgccccgacg gcgagacggt cggctggtgc tgggtgagca
     1741 tcaaccagaa ccagatgacc ggcgaccgtt acgccaactt ccgctccctc gccgtcgccc
     1801 cgctcgacaa ccgcgccgac gtcgccgaac tcctcctcac cgaaggcctg gagttctgcc
     1861 tggccaacgg catcaccgaa gtcgtcggcc gcgtccacgt cggcaacgtc ccgatgcgca
     1921 cggtctaccg caagttcggc ttcgaccccg ccaccctgac catgaagctg cgcctcccgg
     1981 aggaggaatc ccgatgacgg gtgcgacggg cgcgacaggt acgacggatg cgaccggcgc
     2041 acccgacccg accggcgcga ccggcgcgag cagtgcgacc ggcgcgagca gtgcacccga
     2101 cccgaccggt tacgacgccg accggatcaa gtgcgtcgtc tgggacctgg acggcaccct
     2161 gtgggacgag atcgcggtgg agacccccac cgacgaactg ccggtccccc ggcccgaggt
     2221 gctggccgcg atcgacgcgc tggcggcccg cggggtgctc agcagcctcg ccagccgcag
     2281 cgcgcccacg gtcctcgacc ggatggacgc cgtgcccggc ctccgggagc gcttcctcgc
     2341 cccgcaggtg tcctggcagg acaagagcga gtcgctgcgc cgcatcgcca aggacctcgg
     2401 gatcgccgtc gaggcactgg tcctgatcga cgactccccg tacgagcgtg ccgaggtgga
     2461 ggcgctgctg ccgggcgtgc gtaccctcga ccccggcgaa gtcccggcgc tgctggcggc
     2521 gttcgaggac cgcgaggtca cccccgagtc gcgggagcgg gtgcgccgct accgcaccga
     2581 ggagacccgc cgggccgagg gcgagcgctt ccagggcacc cgggaggagt tcctgcgctg
     2641 gtgcgacatg cgcctcacgg tcggcccggc cgtcgccgag gaggtcccgc gggcactgga
     2701 actcgccgcg cgcacccacc gtctgaactc ctccggcctg acccccgacc ggctccgcgc
     2761 gctcgccgcc gcggacgggc acgagctgta caccgcccgg atgtccgacc gcttcgggga
     2821 gtacggcatc atcggcgccg ctctcgtcga ccggaccgcc cgcggttcct ggtcggtgcc
     2881 gctgctggcg ctctcctgcc gggtcgccgg ccggggcgcc gcggcggcgt tcctgttctg
     2941 gctgatggag cgggcgcggg agtccggtgc cgaggacttc cgggtgacgc tgcggccgac
     3001 cgacgccaac ctggagatgc ggatcctgct ccgccaggcg ggcctgcgcc gtacagaagc
     3061 cgacgacagc gccgatggca gcaccgccgg cgacgacggg gcggtcctgg cccgaaccct
     3121 cgacagcggt ggcggtggcg gcggcctgcc gaagcccccc gcctggctgc atgtcttcac
     3181 ccgaaaggac gccgacgcgt gaccgacgcc cacaccccct cctccacctc ctccgcggac
     3241 cgcgcggcgg tggaggagga gcttcgcgcg atgatcgcgg aggccgcccg tctggacgcc
     3301 gccgtggtgg caggactccc cgccgacacc gacctgttcg gcccggagat cggcctgacc
     3361 tcgctggccg gggtgacgct gctgggcgcg gtcgacgccc gcttcggcgt cgatgtcgcg
     3421 gcgctggacc tcagcctgga cagcctccag tccatcgcca ccctggccga cttcgtgacg
     3481 gcgcaccgga gcgcccactg aggcgcactc ccccgcgcgt gcgcgacaag ggtgatccgg
     3541 cgggtgccgc cgtgacctgc ccggggggcc ggcgttgaac ggggagtgcg gtggcgcgat
     3601 ctgctgccgt gcgaaccgaa ccacaaagga gaatgtgatg tctcgcatcg cgaaggcagc
     3661 cgctgtcgcc gtcggcgccg gcgccatgat ggtcagcgga gccggcctgg caatggcgga
     3721 cgcggacgcg cagggtgcgg ccgttggctc gccgggcgtc ctgtccggca acgtggtcca
     3781 ggtcccgatc cacgtgccga tcaacatctg cggcaacacc gtcaacatcg tcggcctgct
     3841 caacccggcc ttcggcaaca cctgcgccaa cgtcagcaac cacgacaagg gcgaggacaa
     3901 gggcgaggac aagggcggcc agcaccacag cggcggcgac gccagtggct acggcggctg
     3961 acgtcagctc gcagcagcac ccctaaggga gagccccggc actcgagcgc cggggctctc
     4021 cccttttccc gtgcctccct cacgcctacc ggcagctttc cctgccgcct ctcatgtacc
     4081 ggcagccctc acacgtaccg ccggctttcc cgtgccgcct cacgcgtacc gtcggccttg
     4141 ccgtgccgcc tcacgcgtac cggtagatcc gtcccgcgtc ctccagctgg tcgggggtca
     4201 cgttccacgg aggcagcctc tggtgccggg cgacgacccg gcggaactgg ctgctgtccg
     4261 gcagcggcgg ttcggcgggc aggtagcggt gtgtcctgtg gcgtcgctgc cagcgggtcc
     4321 acagaaggtc gatgaaggcg tggtggagcc agaacaccgg gtcgttgacg gacgccccgc
     4381 cgaccatggc cccgccgacc caccggtgca cccggttgtg gttgagccag gcggtgctgc
     4441 cccggccggt cccccacccc tccagcttgt tgcggaagcc gcgggtcacc gtcgagtccc
     4501 agggcgagac gtcgtagacc gggtcgctca gcgcccactc cacgtcggcc ttcgtcggca
     4561 gcccgatcgg gtcgcgggcc cggccgaggt cgcgggtgag gaacttcgtg tcggtgacgc
     4621 cgtccctgat cgtccagttg ccggtggcgt gggcgaacgg ccccgtcatc acctgccggt
     4681 ccgagcgccg accgttgccg ccgagcaggt ccgcggtcca cggcacggag gtcgccgccc
     4741 ggtcccgcgt ccagtcccag tacggcaccg tcaccgaggg atcgacccgg cgcagcgcgt
     4801 tctccagatc cagcaggaac cggcggtgcc agggcaggaa ggagggcgtc atgtgggccg
     4861 tgcgcaggcc acgttcgccg tcggaggtgt agtagtcgat gtgcatgcgc acgaactcgt
     4921 cgtactcgcc ccggcgtttc agctccagca gggctcggac gaagcgtcgg cgttcgccgg
     4981 tggtgagtgt gctgacgtcc ttacggatgt aggccatggt ctccccccat gtgtgtgtga
     5041 cggccggtgg cggcggcggg ccgcagcatc tgtccggggc ccagttcgtc gacggcggct
     5101 cggaccgcgt cgagcggatc ggcgtacgag cggtagtggt cgaccatgct cagccaggtg
     5161 ccgtcggcgc ggcgcatgag gtgcaggggg cggccgtcga cggtgatctc ccagcggccg
     5221 gtgtcggcaa cgccgaccgg cggaacgagg cggcccagca ggtggcggcc gaggtaggtc
     5281 tcgtcgaagg cgtttacccc gagcccgagc ccaagcgcgg gcgcgggcgc atggccgtat
     5341 ccgtgaccgt ggccctggcc tttctggcgg cgggccggcc gggaggcggc gaccagcggc
     5401 gcgagtgcga gggccgcggt ggcgccgagc agtccgcgca gcgcactcct gcgtgttccg
     5461 cgtctcgcct tccgtgccgg gcccgtggtc acggcggcga ctccgtccac actgacgacc
     5521 atgtgtgctc ctcgtccggt tccgttgtca cagcggtaac cgtccggcgg cccgcggggt
     5581 caccgtgcgg accggaaagc ggccgggaaa ggcgacaggc ccggacggcg ggtccgggcc
     5641 tgtggtcggg tgggccggcc ggttacaggc cctggccgac cccgaggtcg gcgcccggca
     5701 ccgtctggac gaccggggtg agcacaccgg cctgcggcag ctgaaggtca ggcaggccga
     5761 accggtcggc gttcggtgcg gtgagcgggg cgtccagggc gaggccgggg ccgagggtgc
     5821 gcacgtcgga ggcggggacg tcgaggccga cgtggttgaa cccgtcgccg aggacctccg
     5881 gcagcggcga gcgcatgccc agaccgggaa gcccggcggc gaccggcagc tgcgggatca
     5941 cgcgctccgg gatgaggcgg ccctcgacgt accgcgggcc ttcgggggcg cccggcaccg
     6001 gcaccgggat ctcgccgccg atcttgggca gttccacgtg gagggacttc tcgacgccgt
     6061 ccagcgggac ggggacggga accgtgccga ccgcggcggc tgaggtcgcg gagacggcgc
     6121 cggccgcggc cgccacacag gtgagcacgg cggcgagggt tcctcgggtg gtcgacttca
     6181 tctcgggtac ggtccctttc ggagtgctgg acgggctgtg cctgcgtacc gggtaacgag
     6241 ccggaaaacg acatcagttc acaaacccct cggatgccgc attcgtacga cagtgggatg
     6301 gtcgggccgt ccggaaatgc caccggcagg tcgtatgtgc ggtgtccctg gtgggccgtg
     6361 gcgcacgccg cgggcgcccg gggccggggt cgggaatcct gcggtcgtgg cgatccacgc
     6421 cggagcgcat cggcggcacg gcacgttcgc ccaggtgccc ggagtcaggc cgggaccgga
     6481 cgcccggatc cggacaggac acaggacagc accggcgcga cagcagggcg gggggtcagc
     6541 gccgggtgcc ggcttgggat ccagggactt cgcggctcgg ccagcgggga cgacccgcga
     6601 ggccgccacg ccgccactcc aacactccgg cactgcgggc acaccgccac accggggagt
     6661 gccgacgaca gcgtgggcca ccggccgcca ccggccgccg ccaggcaccg tcgaacccgt
     6721 caggcaccgc cgagcctgtc aggcgccgcc gagcccgcca ggcaccgtcg aacccgtcag
     6781 gcaccgccga gcctgtcagg cgccaccgag cccgccagac accgtcgaac ccgtcaggca
     6841 ccgccgagcc tgtcaggcgc caccgagccc gccagacacc gtcgaacccg tcaggcgcca
     6901 ccgagcccgt caggcgcgca ggctacgggc gggcaggatg atgtgcgcgg ctgccttcag
     6961 ggtctcctcg tcgccggcga acgtgttcag agcctcggcg gcgaccgtcg aaccgggcgc
     7021 gtctccgggc caggggcggg tggtgaagag gaggtaggca tagccacgct cggccaccgc
     7081 ctcgagccac tccggtggca ccgtgcactg ggtcctgagc catggcatgg tcacgacggc
     7141 ctggccggcc acgaccagca tggtgacggg cagactcagc ttctgggtgc cgtcggtgat
     7201 accgcccacg accaggccgc tgaattccag cagcctctcg acggccgcgg tcgcgccctc
     7261 atggccaccg ggaccgttgc cgagggaaca ggcgaggagg tagggcatcg cgcggccgtc
     7321 cgcggcctcg ccgctccacg gcatcatgac gagggttccg aggtcggcga cggggaaggg
     7381 gcgggttgcg ccgggggttg tggtcaccgc gcgaccctaa ggcgtcccgg cggtccgccc
     7441 ggacggttgt cacccgggcg gaggacaccg agcgggtgct ccacacgaac gagggaccgg
     7501 tccgccggcc cgcctcgaac aacgcggacg gccggtcccg tccgtgacgc gaccggccgt
     7561 ccggaaggcc tccgggcggt gacgtcagcc ctgcaggggc aggccgccga ggagcgactt
     7621 cggggcgttg agcgcggtgg tcgcgtcctt gacgccgctg agcggggagt tcttgttctc
     7681 cgtgtcgagc gtgtccgact ggtgctgcag cggcatcacg tcgatggggc ccttggtgag
     7741 cgtgttgacg gcgccgttga ggctggtggg cgtgaggtcg gcggtgtcgt gcgcgaatgc
     7801 cggtgcggcg gcacctgcga tcacgaggga tccggcaacg acagcggcag ccttcaggga
     7861 cttcatcggg gacctttctt cggcaactcg gcaactcggc aactcaagtc accagcgaga
     7921 ctctgacgcc gtgtctaacg atgtgcggcg acggcggaaa ccccggacgc ggaagaattc
     7981 cgcaccccgg cacgggccca cccgaacgcc gaggtgcgga aaggtgaacc ggccgtcagg
     8041 cggcgggggc ctgccccttc ggggtgagcg gcggcacctg tgccggcagg gtggacggcg
     8101 tgaccggcag ggccggggtc gtcaccggca gggccggggt ctcggccggc agggtggaga
     8161 cctcgggaag gtcgcccagg ccctccaggc ccgtcaggcc cgtcaggccc gtcagggtcg
     8221 gcagggtcgg cagggtcggc agggtcggca gggtcggcag ggtcggcagt gacaggccgt
     8281 ccaccaggga ggtgaccagg tcgctcaggc ctgtcaccag gctcgtggcc gcggggagga
     8341 tctggccgac gtcgccggag gtggccgcct cgagcagcgc gtcgagctgc ctctccacgg
     8401 tggcgagcag gtcgtccgtg atgtcggcgg cccgggcccg tgccccgggg ccgacgcgat
     8461 caccgtggga cggcgtcgcg gccgggccgg tggactcggc cacccgtgtc agagcgtcct
     8521 cggcggcggc gcccagcctc ttcgcccggt cggcgctcct gccctggctg tcggccctca
     8581 gcgaggtgtc gagcaggtcg gtgacgggag cgagcgcggg tttgacgtcg tccaggctcc
     8641 gggccctggc gagcagcgcg tccgcgccgg agagagacgc actcggcacc gcatgagtgc
     8701 gctcgcgggt ggcttcccca tcggcggcca gggcggaggg agcggcgatg ccgagaagga
     8761 gggtgacgca cagcgcggtg aaagcggtac gccgaacggg cagaccacgc atgggggttc
     8821 ctttcgatat gcgtcgagtc gcaggttcag cgtgaaagcg catgtcacgc gccgcaacag
     8881 cacggtcact ttccgtcacc tccgcgtgtg acgtgccccc cccctggtgg gacggcacgg
     8941 cgcggtccac cgggcggtcg cggcaccctc ccgccattcg tggcgacacg cgggagcgac
     9001 ggcgcgacac cttgggccgg acgggtgggc catccgtccg gacaacgcga acggccgccc
     9061 tcctgacgga agggcggccg tctgacgtcg cacgcgtcgt gacctcggtc gaggtgacgt
     9121 cagtcgttga tgcaggtgtt gccgaacgcc gggttcagca gcccgatcac gttgacggtg
     9181 ttgccgcaga cgttgaccgg aatgtggatc ggaacctgga tgacgttgcc cgacagcacg
     9241 cccggggagt tggcggcgac agctttggcg ccactgtcgg cggcggcgat gccggaggcg
     9301 ccggcgacgg ccgcggcggc aacggaggtc aggaccaggc ccttcgcgat acgcgacatg
     9361 gagaggtgct ccttgggggt tttgacacat acacccgggc ggggatgccc ggcggtgtgt
     9421 caacgcgcga aagcgcccgg agttttgcct ccgaacgggt gacgtgacgg tgccacggtc
     9481 ttcacagctc cgacacagat gaccgacttc tccttattaa tcctgattgt gactagagtg
     9541 acggctcgtc ccgcccgctt gtcgccagca caccgcacct tttctgaaag ggccacgccg
     9601 gtcatgcgcc attccctggg tccgctgctg cgccgggcgt cggtgggctt cctcgccctc
     9661 gcctccctct gcgccccggg cggcgccctg gccgccccgg cgccgccctc cgccgaggcc
     9721 gagcgcctcg ccttcaccca gcggtaccgc gcgacccagc acggcggcat cgcccgggcg
     9781 gccaactcgg ccgtcagctg ccgtacgaca ccggcgtccg ccacccgaac cacgcctgcc
     9841 tgcacggctg tccgggaggg gcgggcggcg gccaacggcg acttcgacat gttctacgtc
     9901 gatgtcgacc gcgacccgaa cacctacaac tcctcccgcg ccgaggtccg cctccccgag
     9961 ggctcccggg tgacgtacgc gcggctgtac tggggcggca atctgcgcgt cggtgagcag
    10021 aagccgccga aggacaacgg gcgggtgctc gtcgcggagc cgggcggcgc gtacaaggag
    10081 gtgctcgcgg acaccgtcac cggccaccgg gtggcgcggg gcgccgatgc cttccaggcc
    10141 tccgcggacg tcacgggact ggtgcgcgag agcggggcgg gcctgtacac ggtggcgcag
    10201 gtcaacgtgg cgatgggcag gtccgcggcc ggtgcgtggg gcggctggac gctggtggtg
    10261 gcgtacgaga acccgcggga gccgttgcgg cacctcgcgg tgtgggacgg cttcgacgcg
    10321 ctcggcccgg gcgcggggca ggaggtccgg ctgcgcgggg tgccgttccc acgcggtgcg
    10381 ggcggccggg cggggctggt cgcctacgac ggggaccgcg gccgttcggg tgattcgatc
    10441 acgttctcga ccggctcgcg gggggtctcg gcgctctccg acccggtcaa tccatctgac
    10501 gatgtgttga actccacgat ca
//
