LOCUS       NZ_CP099468            10405 bp    DNA     linear   CON 04-JUL-2024
DEFINITION  Streptomyces phaeoluteigriseus strain Qhu-M197 chromosome, complete
            genome.
ACCESSION   NZ_CP099468
VERSION     NZ_CP099468.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces phaeoluteigriseus
  ORGANISM  Streptomyces phaeoluteigriseus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group.
REFERENCE   1  (bases 1 to 9026270)
  AUTHORS   Zhang,B., Xiang,X. and Fan,J.
  TITLE     Complete genome sequence of soil microorganisms Streptomyces sp.
            Qhu-M197 isolated from Alpine meadows habitats on the Tibetan
            Plateau
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9026270)
  AUTHORS   Zhang,B., Xiang,X. and Fan,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2022) Faculty of Eco-Environmental Engineering,
            qinghaiuniversity, No. 251 Ningda Road, Chengbei District, Xining
            City, Qinghai Province 810000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Hifiasm v. v0.14; Circlator v. v1.5.5; Pilon v.
            v1.22
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 68.51291x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023923245.1-RS_2024_07_04
            Annotation Date                   :: 07/04/2024 06:06:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,100
            CDSs (total)                      :: 8,006
            Genes (coding)                    :: 7,707
            CDSs (with protein)               :: 7,707
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 299
            CDSs (without protein)            :: 299
            Pseudo Genes (ambiguous residues) :: 0 of 299
            Pseudo Genes (frameshifted)       :: 130 of 299
            Pseudo Genes (incomplete)         :: 241 of 299
            Pseudo Genes (internal stop)      :: 34 of 299
            Pseudo Genes (multiple problems)  :: 90 of 299
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:40:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6735459
            Orig. end                         :: 6745864
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP099468.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="30"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(18..869)
                     /locus_tag="NFX46_RS29950"
                     /old_locus_tag="NFX46_29950"
     CDS             complement(18..869)
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121392.1"
                     /locus_tag="NFX46_RS29950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_29950"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_252553531.1"
                     /transl_table=11
                     /translation="MNTLLGRRTRVLAATTATAGLMLVAACTSSDDGGSGSKTAAGGVE
                     LVKAGQLTTCTHLPYPPFQSEIDGKVQGFDVSVIDLVADDLGVKQEILDTPFENFKTGA
                     FLNSGECDLAAAGMTITEERKKNVDFSDPYFDATQAVLVNKDAGVDSLAEIKSGNVKLG
                     AQAQTTGEDYVTKQGFDPVSFESSDAVLNGLRTGQVKAVVIDYPVVQGWLKDKANADAF
                     EVVDNLNTGEQYGFTVKKGNTQLVAAINKAIADARADGTYKKLYEQWIGPYDATAASPS
                     AS"
     gene            1024..2145
                     /locus_tag="NFX46_RS29955"
                     /old_locus_tag="NFX46_29955"
     CDS             1024..2145
                     /NRPS_PKS="Domain: Aminotran_5 (81-344). E-value: 5.2e-26.
                     Score: 83.3. Matches aSDomain:
                     nrpspksdomains_NFX46_RS29955_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121393.1"
                     /locus_tag="NFX46_RS29955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_29955"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_252553532.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1e-19, bitscore:
                     69.6, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEESTAVTHPFLDLAPLSAARFASVEDRVARLLDTRQDVVIMQG
                     EALLPLEGAIRGTAGPGTTALNVITGPYGQTFGDWLRDCGATVIDLAVPFHTAATAGQI
                     RAAFTEHPEIGFVSLVHAEAATGNTNPVAEIGEVVKEHDALFYLDAVASVGAEPVLPDA
                     WGVDLCVIGAQKAMGGPAGVSAVSVSERAWARMAANPKAPRRSYLSLLDWKERWLDGGR
                     KALPHAPAQLEMLALEACLERIEADGLDTVMARHASAAAATRAGAVALGAGLEPYVHEA
                     REAAPVATTLRAPAGVVASELVARALASDPALPLAAGGGALAKEMIRVNHYGADATPGA
                     VRSSLTALGAALAERGLDVDLTGALRAGEQAWR"
     aSDomain        1267..2055
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NFX46_RS29955_Aminotran_5.1"
                     /evalue="5.20E-26"
                     /label="NFX46_RS29955_Aminotran_5.1"
                     /locus_tag="NFX46_RS29955"
                     /protein_end="344"
                     /protein_start="81"
                     /score="83.3"
                     /tool="antismash"
                     /translation="RDCGATVIDLAVPFHTAATAGQIRAAFTEHPEIGFVSLVHAEAAT
                     GNTNPVAEIGEVVKEHDALFYLDAVASVGAEPVLPDAWGVDLCVIGAQKAMGGPAGVSA
                     VSVSERAWARMAANPKAPRRSYLSLLDWKERWLDGGRKALPHAPAQLEMLALEACLERI
                     EADGLDTVMARHASAAAATRAGAVALGAGLEPYVHEAREAAPVATTLRAPAGVVASELV
                     ARALASDPALPLAAGGGALAKEMIRVNHYGADATPGAVRSS"
     gene            2304..2816
                     /locus_tag="NFX46_RS29960"
                     /old_locus_tag="NFX46_29960"
     CDS             2304..2816
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140784.1"
                     /locus_tag="NFX46_RS29960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_29960"
                     /product="DinB family protein"
                     /protein_id="WP_252553533.1"
                     /transl_table=11
                     /translation="MTTLPDGRPVPLMTGDERAMLDSWLDFHRATLKLKCAGLDDTQVR
                     QASAEPSSLTLLGLVQHLAEVERNWFQRVFAGLDVSPVFEEPTGYALSPERGLGEALTV
                     WQREIARGRELTAGRSLDDTGRIDGGPAAGAEVSLRWVLIHLVEEYARHNGHADILRER
                     IDGVTGA"
     misc_feature    2922..2935
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.91"
                     /tool="antismash"
     misc_feature    complement(2945..2960)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.8"
                     /tool="antismash"
     misc_feature    complement(2989..3001)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.42"
                     /tool="antismash"
     misc_feature    3052..3064
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.12"
                     /tool="antismash"
     misc_feature    3055..3064
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.15"
                     /tool="antismash"
     gene            3089..3598
                     /gene="ectA"
                     /locus_tag="NFX46_RS29965"
                     /old_locus_tag="NFX46_29965"
     CDS             3089..3598
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381146.1"
                     /locus_tag="NFX46_RS29965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_29965"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_252553534.1"
                     /transl_table=11
                     /translation="MTATQADLRIDRPSAADGAALWRIAKESGTLDLNSSYSYLLWCRD
                     FADTSAVARAESGEPVGFVTGYVRPQAPETLLVWQVAVDGAYRGRGLAATLLDALTARV
                     AAERPLTSVETTITPGNTASERLFTSYAARHGADVTREVLFDTGLFPDGPHDPEVLYRI
                     GPLTSV"
     gene            3670..4959
                     /gene="ectB"
                     /locus_tag="NFX46_RS29970"
                     /old_locus_tag="NFX46_29970"
     CDS             3670..4959
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 8.1e-73.
                     Score: 237.3. Matches aSDomain:
                     nrpspksdomains_NFX46_RS29970_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="NFX46_RS29970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_29970"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_252553536.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.2e-77, bitscore:
                     259.3, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPVVFDRAQGSRMHDEDGHQY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLMRDGVTHGLDMSTVAKRTFLQTFQDLVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GVPLVHGTPMPFDNYFDGQVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALAELCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMS
                     LCLFKPELDVWEPGEHNGTFRGNNPAFVTATAALETYWADGSAMEKQTRTRGEQIEQEL
                     ISITEENLADVKEYRGRGLVWGLEFHEKARAGRVARRAFELGLLIETSGPEGEVVKLLP
                     ALTVTPEELDEGMSVLARAVRETVAVPETA"
     aSDomain        3754..4767
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NFX46_RS29970_Aminotran_3.1"
                     /evalue="8.10E-73"
                     /label="NFX46_RS29970_Aminotran_3.1"
                     /locus_tag="NFX46_RS29970"
                     /protein_end="366"
                     /protein_start="28"
                     /score="237.3"
                     /tool="antismash"
                     /translation="FDRAQGSRMHDEDGHQYLDFFAGAGSLNYGHNNPVLKRALIDYLM
                     RDGVTHGLDMSTVAKRTFLQTFQDLVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGVPLVHGTPMPFDNYFDGQVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALAELCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTVSKSISGYGLPMSLCLFKPELDVWEPGEHNGTFRGNNPAFV
                     TATAALETYWADGSAMEKQTRTRGEQIEQELISITEENLADVKEYRGRGLVWGLEFH"
     gene            5001..5405
                     /locus_tag="NFX46_RS29975"
                     /old_locus_tag="NFX46_29975"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="NFX46_RS29975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_29975"
                     /product="ectoine synthase"
                     /protein_id="WP_252553537.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.9e-53, bitscore:
                     178.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVRSASGTWESKRIVLAKERVGFSLHETILY
                     AGTETSMWYANHIEAVVCVEGEAELTDHETGLAHTITPGTMYLLDGHERHTLRVKQDFR
                     CICVFNPPVTGREDHDENGVYPLLTEPEEV"
     gene            5413..6315
                     /gene="thpD"
                     /locus_tag="NFX46_RS29980"
                     /old_locus_tag="NFX46_29980"
     CDS             5413..6315
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976953.1"
                     /locus_tag="NFX46_RS29980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_29980"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_252553539.1"
                     /sec_met_domain="PhyH (E-value: 7.7e-40, bitscore: 136.2,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVTGTHVTDLYPSRGATEVRVPRQDPVVWGAPDAPGPFEPLDL
                     QSFERDGFAAVDQLLTDDEVAVYKRELDRLVDDPAIRADDRSVVEPRTKEIRSVFEVHR
                     ISEVFARLVRDERVVGRARQLLGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGL
                     PRMRTVSVSIALTENHDTNGGLMIMPGSHKTFLGCAGATPKDNYKQSLQMQDAGTPSDE
                     ALTEMASAYGIKLFTGRAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENAAVEPF
                     AAPVRRPEFIGARDFTPVR"
     gene            complement(6430..7482)
                     /locus_tag="NFX46_RS29985"
                     /old_locus_tag="NFX46_29985"
     CDS             complement(6430..7482)
                     /NRPS_PKS="Domain: Aminotran_5 (17-344). E-value: 6e-24.
                     Score: 76.5. Matches aSDomain:
                     nrpspksdomains_NFX46_RS29985_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989745.1"
                     /locus_tag="NFX46_RS29985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_29985"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_252553540.1"
                     /sec_met_domain="Aminotran_5 (E-value: 3.9e-18, bitscore:
                     64.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFETLVRAEFAPKNTYLNTASNGLLPARTVTALHEAALLRAEG
                     RPLTPLYEDVEASRAAYARLVGVPVTRVAAGASVAAHTGLIATSLPAGAEVLTAEDDFT
                     SVLNPFHVRGDLKVRAVPLERIAEAVRPGTALVAVSAAQSADGRIADLPALREAARTHG
                     ARTYVDFSQSAGWLPMDADAYDYSVSVSFKWLLGPHGAAFLVVPEDFGDLTPVLAGWVA
                     AEAPWDSCYGPVAELAHSARRFDLSHALFTYAGLRRSLDLIEELGVAAVRAHDLALADR
                     FRAGLAALGHEPVPAPGSAIVSVPGLGVRQPELSRAGIEVSNRAGNLRASFHLFNTTAD
                     VDRLLDVLSG"
     aSDomain        complement(6451..7431)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NFX46_RS29985_Aminotran_5.1"
                     /evalue="6.00E-24"
                     /label="NFX46_RS29985_Aminotran_5.1"
                     /locus_tag="NFX46_RS29985"
                     /protein_end="344"
                     /protein_start="17"
                     /score="76.5"
                     /tool="antismash"
                     /translation="YLNTASNGLLPARTVTALHEAALLRAEGRPLTPLYEDVEASRAAY
                     ARLVGVPVTRVAAGASVAAHTGLIATSLPAGAEVLTAEDDFTSVLNPFHVRGDLKVRAV
                     PLERIAEAVRPGTALVAVSAAQSADGRIADLPALREAARTHGARTYVDFSQSAGWLPMD
                     ADAYDYSVSVSFKWLLGPHGAAFLVVPEDFGDLTPVLAGWVAAEAPWDSCYGPVAELAH
                     SARRFDLSHALFTYAGLRRSLDLIEELGVAAVRAHDLALADRFRAGLAALGHEPVPAPG
                     SAIVSVPGLGVRQPELSRAGIEVSNRAGNLRASFHLFNTTADVDRL"
     gene            complement(7595..8311)
                     /locus_tag="NFX46_RS29990"
                     /old_locus_tag="NFX46_29990"
     CDS             complement(7595..8311)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140791.1"
                     /locus_tag="NFX46_RS29990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_29990"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_252553542.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALDAFPHRDQVEVVHRSFELDP
                     GRAKGDVQPVLAMLTKKYGMSEAQAQAGEDNLGAQAAAEGLDYRTRDRDHGNTFDMHRL
                     LHFAKEQGRQDELIQLLYRANFAEERSLFGEGDERLVELAAEAGLDTDAARKVLADPAA
                     YADEVRADEAEAARLGANGVPFFVLDRTYGVSGAQPAEVFAQALTQAWGERSPLKLVDQ
                     GDAQACGPDGCAVPRH"
     gene            complement(8472..9080)
                     /locus_tag="NFX46_RS29995"
                     /old_locus_tag="NFX46_29995"
     CDS             complement(8472..9080)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="NFX46_RS29995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_29995"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_073494151.1"
                     /transl_table=11
                     /translation="MDLELAELPFPLRTYGPDGHWSYEDGVLTGWGGPRQDRFVPPTGE
                     ALDPAADAPRLLGAPEGDFQFIARVTVGFNGAFDAGVLYVHVGERAWAKLCLEYSPDVP
                     TVCTVVTRGHSDDANSFAVDGSSVWLRVSRTGRAFAFHASRDGEHWVFVRLFTLGDEKE
                     TGAALVGFMAQAPMGQGCVVTYDHLEFRPSWPDDLRDGS"
ORIGIN
        1 cggcttcgtg gcgtcgctca tgaggcggac ggggaggccg cggtcgcgtc gtacgggccg
       61 atccactgct cgtagagctt cttgtaggtg ccgtcggccc tggcgtcggc gatcgccttg
      121 ttgatcgcgg ccaccagctg ggtgttgccc ttcttcaccg tgaagccgta ctgctcaccg
      181 gtgttgaggt tgtcgacgac ctcgaaggcg tcggcgttcg ccttgtcctt cagccagccc
      241 tggacgaccg ggtagtcgat gacgacggcc ttgacctggc cggtgcgcag gccgttgagg
      301 acggcgtcgg aggactcgaa ggagaccggg tcgaagccct gcttggtcac gtagtcctcg
      361 ccggtggtct gcgcctgcgc gccgagcttg acgttcccgg acttgatctc ggcgagggag
      421 tccaccccgg cgtccttgtt caccaggacg gcctgggtgg cgtcgaagta ggggtcggag
      481 aagtcgacgt tcttcttccg ctcctcggtg atggtcatgc cggccgcggc caggtcgcac
      541 tcgccggagt tcaggaaggc gccggtcttg aagttctcga acggcgtgtc caggatctcc
      601 tgcttcacgc ccaggtcgtc ggcgaccagg tcgatcacgg agacgtcgaa gccctgcacc
      661 ttgccgtcga tctccgactg gaacggcggg tagggcaggt gggtgcaggt ggtgagctga
      721 cccgccttga ccagctccac accgcccgcg gcggtcttgg agccgctgcc gccgtcgtcg
      781 ctggaggtac aggcggccac gagcatgagc ccggccgtag cggtggtggc ggccaggaca
      841 cgggtgcgac gcccgaggag cgtgttcacg gcgggccttc ctgtgggaga actgtggctt
      901 ccgattataa ggagaagttt gaggctctca aatcaaaccg ttggcagcgg ggtccgtcgg
      961 cctaagaagt cgccggtccg gggtggtggg ggtcacgccg gttactctcg tcacccttac
     1021 cccgtgagtg aagagagcac cgccgtgacc caccccttcc tggatctcgc tccgctgagc
     1081 gccgcccggt tcgcctccgt cgaggaccgg gtggcacggc tgctggacac ccggcaggac
     1141 gtcgtgatca tgcagggcga ggcgctgctg ccgctggagg gcgcgatccg cgggacggcc
     1201 gggccgggca cgaccgcgct gaacgtgatc accgggccct acgggcagac cttcggcgac
     1261 tggctgcggg actgcggcgc cacggtgatc gacctcgcgg tgcccttcca cacggcggcc
     1321 accgccggtc agatccgcgc ggccttcacc gagcacccgg agatcggctt cgtgtcgctg
     1381 gtgcacgccg aggcggccac cggcaacacc aacccggtcg cggagatcgg tgaggtggtg
     1441 aaggagcacg acgcgctgtt ctacctggac gccgtggcgt ccgtgggcgc cgagccggtg
     1501 ctgccggacg cgtggggcgt ggatctgtgt gtgatcggcg cgcagaaggc gatgggcggc
     1561 ccggcgggcg tgtcggcggt gtcggtgagc gagcgggcgt gggcccggat ggcggcgaac
     1621 ccgaaggcgc cgcgccgctc ctacctctcg ctgctcgact ggaaggagcg gtggctcgac
     1681 ggcggccgca aggcgctgcc acacgctccg gcacaactgg agatgctggc gctggaggct
     1741 tgcctggagc gcatcgaggc ggacggcctg gacaccgtga tggcccggca cgcgtccgcc
     1801 gccgccgcga cccgcgcggg tgcggtggcg ctgggggccg ggctggagcc gtacgtgcac
     1861 gaggcgcggg aggccgcacc cgtcgccacg acgctgaggg cgccggcggg tgtcgtcgcc
     1921 tcggagctgg tggcgcgggc gctggcgagc gacccggcgc tgccgctggc cgcgggcggg
     1981 ggcgcgctgg ccaaggagat gatccgggtc aaccactacg gggcggacgc gacgccgggc
     2041 gcggtccgca gcagcctgac ggcactgggc gcggcgctcg cggagcgggg cctggacgtg
     2101 gacctcaccg gcgcgctgcg agcgggggag caggcctggc ggtgaggagg gcgccgggtc
     2161 ggcatgatcc ggggggggcg ggtcggcgtg atcgggggcg ggtcggcgtg aatcgggggc
     2221 ggatcggcga ggccgaaaag ggaagccggt cggccggtcg ctctcgtggc cggggtgtca
     2281 gtgggccctg ccatgctcgc cgcatgacca ctctcccgga cggccgtccc gtccccctga
     2341 tgaccggtga cgagcgggcc atgctcgaca gctggctcga cttccaccgc gcgacgttga
     2401 agctgaagtg cgcgggcctg gacgacaccc aggtccggca ggcctcggcc gagccctcct
     2461 ccctcacgct gctcggtctg gtccagcatc tcgccgaggt cgaacggaac tggttccagc
     2521 gggtgttcgc gggactcgac gtatcgcccg tgttcgagga gcccaccggg tacgcgctca
     2581 gccccgagcg gggactgggc gaggcgctga cggtgtggca gcgggagatc gcgcggggac
     2641 gcgagctgac cgcggggcgc tcgctggacg acaccgggcg gatcgacggc gggccggcgg
     2701 ccggggcgga ggtcagtctg cgctgggtgc tgatccacct cgtcgaggag tacgcgcggc
     2761 acaacggcca cgcggacatt ctgcgggagc gtatcgacgg ggtcaccgga gcctgaattc
     2821 acggtaattc cgcagttcct ttcccccgcc tttctctcac cctttcccag gatgggtttc
     2881 ccgagttctc ctgcccggat cttccgggcc caaacgcaaa gatttttcga gcacattacg
     2941 agcgcgtgac gcattgcaca gaggtagccg tttgccccgt tttagccggt gcaaaacgcc
     3001 acaaatcgac acccggccac gagggcatca ccaaaccatc gccgcctctc catgcatttt
     3061 ttaatttccc tcggtaagtt ccatccgcat gactgccaca caagcagacc tgcggatcga
     3121 ccgcccctcg gcggcggacg gggccgctct ctggcgtatc gccaaggaat ccgggactct
     3181 cgatctgaac tcctcctaca gctatctgct ctggtgccgg gacttcgccg acacctcggc
     3241 ggtggcgcgc gccgagagcg gcgaaccggt cgggttcgtc accgggtacg tccgtcccca
     3301 ggcaccggag acgctgctcg tctggcaggt cgcggtcgac ggcgcgtacc gcggccgcgg
     3361 cctcgccgcc acgctgctcg acgcgctgac cgcgcgggtc gccgccgagc gccccctcac
     3421 cagcgtcgag accacgatca cgccgggcaa caccgcctcc gagcggctgt tcacctccta
     3481 cgccgcccgc cacggtgcgg acgtcacgcg cgaggtcctg ttcgacacgg gactgttccc
     3541 ggacggcccg cacgatcccg aagtcctcta ccgcatcggc ccgttgacct ccgtctgagc
     3601 cttccccgga gacccggtcc cccacgacct ccgcacgact cccccaccgc atcgaggagc
     3661 gttttcgccg tgaccatcac ccagcccgac ctcagcgtgt tcgagaccct cgagtccgag
     3721 gtgcgcagct actgccgcgg ctggcccgtc gtcttcgacc gggcgcaggg cagccggatg
     3781 cacgacgagg acggccacca gtacctcgac ttcttcgcgg gtgccggctc tctcaactac
     3841 ggacacaaca acccggttct gaaacgggcg ctgatcgact acctgatgcg cgacggcgtc
     3901 acccacgggc tcgacatgtc caccgtcgcc aagcggacct tcctccagac cttccaggat
     3961 ctggtgctgc ggccgcgcga cctgccgtac aaggtcatgt tcccgggccc gaccggtacc
     4021 aacgccgtgg agtccgcgct gaagctggcc cggaaggtga aggggcgcga ggcgatcgtg
     4081 tcgttcacca acgccttcca cgggatgtcg ctcggctcgc tcgccgtcac cgggaacgcg
     4141 ttcaagcggg ccggggcggg cgtcccgctg gtgcacggca cgccgatgcc gttcgacaac
     4201 tacttcgacg gccaggtccc ggacttcctg tggttcgagc ggctcctgga ggaccagggc
     4261 tccggactca acaagccggc cgccgtgatc gtcgagaccg tgcagggcga gggcggcatc
     4321 aacgtcgcgc ggccggagtg gctgcgggcg ctggccgagc tgtgcgagcg gcaggacatg
     4381 ctgctgatcg tcgacgacat ccagatgggg tgcgggcgca ccggtgcctt cttctcgttc
     4441 gaggaggcgg gcatcacccc cgacatcgtc accgtgtcca agtccatcag cggctacggc
     4501 ctgcccatgt cgctgtgcct gttcaagccc gagctggacg tgtgggagcc gggcgagcac
     4561 aacggcacct tccgcggcaa caacccggcg ttcgtcacgg ccaccgccgc gctggagacg
     4621 tactgggccg acgggtcggc gatggagaag cagacccgca cgcgcggtga gcagatcgag
     4681 caggagctga tctcgatcac cgaggagaac ctcgccgacg tcaaggagta ccggggccgc
     4741 ggcctggtgt ggggcctgga gttccacgag aaggcgcggg ccgggcgggt ggcacgccgc
     4801 gcattcgaac tcgggctgct gatcgagacg tccggcccgg agggcgaggt cgtcaagctg
     4861 ctgccggcgc tcaccgtgac gcccgaggag ctggacgagg gcatgagcgt cctcgcccgc
     4921 gccgtgcggg agaccgtcgc cgtgcccgag accgcctgac ctttccggca caccacctat
     4981 ccaggaggga tcgcaacacc gtgatcgtcc gttcgttcaa ggacatcgaa ggcaccgacc
     5041 ggcatgtgag gtcggcgtcc ggcacctggg agagcaagcg catcgtcctc gccaaggaga
     5101 gggtcggctt ctccctgcac gagacgatcc tgtacgcggg cacggagacg tcgatgtggt
     5161 acgcgaacca catcgaggcc gtcgtctgcg tggagggcga ggccgaactc accgaccacg
     5221 agaccgggct ggcgcacacc atcacgcccg ggaccatgta cctcctcgac gggcacgagc
     5281 ggcacacgct ccgcgtcaag caggacttcc gctgcatctg cgtgttcaac ccgcccgtca
     5341 cgggacggga ggaccacgac gagaacggcg tctatccgct gctcaccgag cccgaggagg
     5401 tgtgacatac ccatgaccac tgtcaccggc acccacgtca ccgatctcta ccccagccgc
     5461 ggcgccaccg aggtgcgcgt cccccgccag gacccggtcg tctggggcgc cccggacgcg
     5521 cccggcccgt tcgagccgct cgacctccag tcgttcgagc gggacggctt cgccgccgtc
     5581 gaccagctcc tcacggacga cgaggtcgcc gtctacaagc gtgagttgga ccggctggtg
     5641 gacgacccgg cgatccgggc cgacgaccgg tccgtcgtcg aaccgaggac caaggagatc
     5701 cggtccgtct tcgaggtgca ccggatcagc gaggtcttcg cccggctggt gcgcgacgag
     5761 cgggtcgtcg ggcgggcccg gcagctcctc ggctcggacg tgtacgtcca tcagtcacgg
     5821 atcaacgtca agcccggctt cggcgcctcc ggcttctact ggcactccga cttcgagacc
     5881 tggcacgccg aggacggtct gccccggatg cgcacggtgt ccgtctcgat cgcgctgaca
     5941 gagaaccacg acaccaacgg cggcctgatg atcatgccgg gttcgcacaa gacgttcctg
     6001 ggctgcgcgg gggccacgcc gaaggacaac tacaagcagt cgctccagat gcaggacgcg
     6061 ggcacgccct ccgacgaggc gctgaccgag atggcgtccg cgtacggcat caagctgttc
     6121 acgggccggg ccgggtcggc gacctggttc gactgcaact gcatgcacgg gtccggcgac
     6181 aacatcacgc cgttcccgcg cagcaacgtc ttcatcgtgt tcaacagcgt ggagaacgcg
     6241 gcggtcgagc cgttcgcggc acccgtgcgg cggcccgagt tcatcggagc gagggacttc
     6301 acgccggtcc ggtgaacccg gccggggtcc ggggggcccg gggggggcgc cccagacgtc
     6361 acacggcatc ggcgcccgcg tcctcacgcc cggtgcaccg agcactcccg cccggcgcac
     6421 ccggcgtgct cagcccgaca gcacgtccag cagccggtcc acgtccgccg tcgtgttgaa
     6481 caggtggaag gacgcgcgca ggttgcccgc gcggttggag acctcgatgc ccgcgcggct
     6541 caactccggc tgccggacgc cgagtccggg caccgagacg atcgccgagc ccggtgcggg
     6601 cacgggctcg tggccgagtg ccgcgagccc ggcccggaac cggtcggcga gggcgaggtc
     6661 gtgggcccgt acggcggcga cgccgagttc ctcgatgaga tcgagggagc ggcgcagccc
     6721 ggcgtaggtg aacagggcgt ggctcaggtc gaaccgcctt gcggagtggg cgagttcggc
     6781 gacggggccg tagcagctgt cccagggcgc ctcggcggcg acccatcccg ccagtaccgg
     6841 cgtgaggtcg ccgaagtcct ccggcacgac gaggaaggcg gccccgtgcg ggccgagcag
     6901 ccacttgaag gagacggaga cggagtagtc gtacgcgtcg gcgtccatcg gcagccagcc
     6961 ggcggactgg gagaagtcga cgtaggtgcg cgcgccgtgg gtgcgggccg cctcgcgcag
     7021 ggccggcagg tccgcgatcc gtccgtcggc ggactgggcg gcgctcaccg cgacgagcgc
     7081 ggtgccgggg cggacggcct cggcgatccg ctccagcggg accgcgcgga ccttgaggtc
     7141 gccgcggacg tggaacgggt tcagcacgga cgtgaagtcg tcctccgcgg tgaggacctc
     7201 ggcgcccgcc gggagcgagg tggcgatgag gccggtgtgc gcggcgaccg aggcgccggc
     7261 cgcgacccgg gtgaccggga cgccgacgag ccgggcgtac gccgcccgac tggcctccac
     7321 gtcctcgtac agcggcgtca gcggcctgcc ctcggcccgt agcagcgccg cctcgtgcag
     7381 ggcggtgacg gtgcgggcgg ggagcaggcc gttgctcgcg gtgttcaggt aggtgttctt
     7441 cggggcgaac tcggcgcgga cgagggtctc gaaggtctcc atggctccac tctgcggccc
     7501 acggaagtcg aagtccatgg cgaattctta cgcggtttcc ctaaggaacg cttatggatg
     7561 ggccctcacc tgcgcgtttc ggggccgcgg cgggtcagtg ccgcggtacc gcgcatccgt
     7621 cggggccgca ggcctgggcg tcgccctggt cgaccagctt gaggggcgag cgctcgcccc
     7681 aggcctgggt gagtgcctgg gcgaagacct cggcgggctg ggcgccggag accccgtacg
     7741 tgcggtcgag gacgaagaag gggacgccgt tcgcgccgag ccgggcggcc tcggcctcgt
     7801 cggcgcggac ctcgtcggcg taggcggccg ggtcggcgag gaccttccgg gcggcgtcgg
     7861 tgtccagacc ggcctcggcg gccagctcca cgagccgctc gtcgccctcg ccgaacaggg
     7921 accgctcctc ggcgaagttg gcccggtaca ggagctggat cagctcgtcc tggcggccct
     7981 gctccttggc gaagtgcagc aggcggtgca tgtcgaaggt gttgccgtgg tcgcggtcgc
     8041 gggtgcggta gtcgaggccc tcggcggcgg cctgggctcc gaggttgtcc tcgccggcct
     8101 gcgcctgggc ctcgctcatg ccgtacttct tggtcagcat ggccagcacg ggctggacgt
     8161 cgcccttggc ccggccgggg tccagttcga aggagcggtg cacgacctcg acctggtcac
     8221 ggtgcgggaa ggcgtcgagc gccttctcga agcgggcctt gcccacgtag caccacgggc
     8281 aggcgatgtc gctccagatc tcgacgcgca tgtctcggct ctctcaggtc tgcgggtacg
     8341 gagactccct ccgccgagta tgtgaacgtt caaaggctcg gcctcattcc ccgctcacgg
     8401 acaccgcggg aaaggtggtg atcgccgggt gctacggccc gcccgtcgca ccggttccgg
     8461 agcggccccg gtcagctgcc gtcgcggagg tcgtccggcc agctcgggcg gaactccagg
     8521 tggtcgtacg tgacgacgca gccctgcccc atcggcgcct gcgccatgaa gccgacgagg
     8581 gccgcgcccg tctccttctc gtcgcccagc gtgaacagcc ggacgaagac ccagtgctct
     8641 ccgtcgcggg aggcgtggaa ggcgaaggcc cggccggtcc ggctgacccg cagccagacc
     8701 gaactgccgt ccacggcgaa ggagttggcg tcgtcggagt ggccgcgggt gaccaccgtg
     8761 cagacggtgg gcacgtccgg ggagtactcc aggcacagct tggcccaggc ccgttcgccg
     8821 acgtgcacgt acaacacccc ggcgtcgaag gcaccgttga agccgacggt cacccgggcg
     8881 atgaactgga agtcgccctc gggtgccccc agcagccggg gggcgtcggc ggccgggtcc
     8941 agcgcctccc cggtgggcgg cacgaaccgg tcctggcggg gaccgcccca tccggtgagg
     9001 accccgtcct cgtagctcca gtgcccgtcg ggcccgtagg tgcgcagcgg gaaggggagt
     9061 tcggcgagtt cgaggtccat gctcagagtt tcccggattc cttctcgtcg atcggtgccg
     9121 gtcagcgctc gaggtggttg ttgaagcgcc ggggcaggcc gagggggttg tcgtcgcgca
     9181 gctcgggggg cagcagggcc tcgggggtgt tctggtaggc gaccgggcgc agccagcgct
     9241 cgatggccgt accgcccacg gaggtggagg tggaggtggt cgccgggtag gggccgccgt
     9301 ggtgctgcgc gggggccacg gccacgccgg tcggccagcc gttcaccagc acgcggcccg
     9361 cgagcggggt gagttcggcg aggatctcgg cgccgcggcc ctcgccggcg gcctcctgcg
     9421 tcgacaggtg gacggtggcg gtgaggttgc cggggagccg ggagaggacg gaccgcgcct
     9481 ggtcgtcgtc gtcgtagcgc gccaccacgg tcaccgggcc gaagcactcc tccaggagca
     9541 ggtcgtacgc cccctcgtcg gcgagcctcc cggccgggac cgtcaggaag cccgcgctca
     9601 cggtgtgctc gccgcccgct ccgggcgtga ccggcgtctc gacctcgggc agctgcgccc
     9661 gctcggcgac accggcgagg aagttgtcgc gcatgcggtg gtcgagcagg acgcccgcgt
     9721 ccgtgtcgct gaccgcgtcg gtcagcgact tgacgaggcc gtcaccggcc gcgccggacg
     9781 gcacgaggac caggcccggc ttcacgcaga actgtccgac gcccagcgtc atcgagccgg
     9841 ccaggccggt cccgatggcc tccgcgcgct cggcggcggc ggcctcggtc acgacgaccg
     9901 ggttgagcga gcccagctcg ccgtggaagg ggatcggcac gggccgcgcg gccgccgcgt
     9961 cgaacagcgc gcgaccgccc cggatggacc cggtgaaacc ggcggcggcg atcagcgggt
    10021 gcttgatcag ctcgacgccc gcctcgaagc cgtgcaccag accgagcacc tcgacgggga
    10081 tgccgtgctc ggcggcggcc cggcggacca ccttggcgac cagctcggac aggcccgggt
    10141 ggtcggggtg ggacttgacg acgacggggt tgcccgcggc cagcgcgctc gcggtgtcgc
    10201 cgccgacgac ggagaacgcg aaggggaagt tggacgccga gtagacggcg acgacgccca
    10261 gcggcacctt gtagcggcgc aggtccggca ccggcggggt cgcggtgtcg tcggggtggt
    10321 tgatgacgac gtcgaggaag gcgccctcct cgacgatgtc ggcgaaggcg cgcagctggt
    10381 agcaggtgcg ggcgagttcg ccggt
//
