LOCUS       NZ_CP099468            11332 bp    DNA     linear   CON 04-JUL-2024
DEFINITION  Streptomyces phaeoluteigriseus strain Qhu-M197 chromosome, complete
            genome.
ACCESSION   NZ_CP099468
VERSION     NZ_CP099468.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces phaeoluteigriseus
  ORGANISM  Streptomyces phaeoluteigriseus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group.
REFERENCE   1  (bases 1 to 9026270)
  AUTHORS   Zhang,B., Xiang,X. and Fan,J.
  TITLE     Complete genome sequence of soil microorganisms Streptomyces sp.
            Qhu-M197 isolated from Alpine meadows habitats on the Tibetan
            Plateau
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9026270)
  AUTHORS   Zhang,B., Xiang,X. and Fan,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2022) Faculty of Eco-Environmental Engineering,
            qinghaiuniversity, No. 251 Ningda Road, Chengbei District, Xining
            City, Qinghai Province 810000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Hifiasm v. v0.14; Circlator v. v1.5.5; Pilon v.
            v1.22
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 68.51291x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023923245.1-RS_2024_07_04
            Annotation Date                   :: 07/04/2024 06:06:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,100
            CDSs (total)                      :: 8,006
            Genes (coding)                    :: 7,707
            CDSs (with protein)               :: 7,707
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 299
            CDSs (without protein)            :: 299
            Pseudo Genes (ambiguous residues) :: 0 of 299
            Pseudo Genes (frameshifted)       :: 130 of 299
            Pseudo Genes (incomplete)         :: 241 of 299
            Pseudo Genes (internal stop)      :: 34 of 299
            Pseudo Genes (multiple problems)  :: 90 of 299
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:40:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2157229
            Orig. end                         :: 2168561
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP099468.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11332
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11332
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11332
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6332](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6332)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    97..111
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.98"
                     /tool="antismash"
     gene            complement(644..2242)
                     /locus_tag="NFX46_RS09685"
                     /old_locus_tag="NFX46_09685"
     CDS             complement(644..2242)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (104-477). E-value:
                     3.1e-23. Score: 74.6. Matches aSDomain:
                     nrpspksdomains_NFX46_RS09685_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133766.1"
                     /locus_tag="NFX46_RS09685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_09685"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_252547862.1"
                     /sec_met_domain="PF00561 (E-value: 1.5e-26, bitscore: 92.4,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAVLYSATGALLLTTLSAVPAGGTPNAVPGVVIAAERAATEGV
                     DFGACTDVPDLPDSMECGKVGVPLDYARPNGKQIELAVSRVRATHRDPHNSKRRVPRQG
                     ALVFNPGGPGASGLHFPLIGMIPEWKRIGAAYDLVGYDPRGVGRSAPLSCEDPQTFFKG
                     PAPSPTHPSETYKRERVARAKAYARGCAQRAGDALRHYHSLNNARDLDVLRAALGEQRL
                     TFMGASYGTYFGALYATLFPSHVRRMVFDAAVNPDPAQIWYRNNLDQSAAFESRWEDFR
                     EWVARHDRVYRLGGTSQAVLRSYERARAKLAAEPAGGKVGPAQLQGTFLQAGYYDDYWP
                     SRAEALSAYLEGDPKLLIALAAPPREAAAEAENANAVYTAVECNDGPWPTDFRVWDRDN
                     TRLARVAPFETWDNVWANLPCAYWRAPRQQPLDVRTGPGELPPTLILAAERDAAAPYDG
                     ALEMHRRLTGSVLVTERDAGTHGIAGGSNRCVNGHLEAYLLEGRLPARRAACAPHAEPR
                     PADRAGKAAAARPTR"
     aSDomain        complement(812..1930)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NFX46_RS09685_Abhydrolase_1.1"
                     /evalue="3.10E-23"
                     /label="NFX46_RS09685_Abhydrolase_1.1"
                     /locus_tag="NFX46_RS09685"
                     /protein_end="477"
                     /protein_start="104"
                     /score="74.6"
                     /tool="antismash"
                     /translation="ALVFNPGGPGASGLHFPLIGMIPEWKRIGAAYDLVGYDPRGVGRS
                     APLSCEDPQTFFKGPAPSPTHPSETYKRERVARAKAYARGCAQRAGDALRHYHSLNNAR
                     DLDVLRAALGEQRLTFMGASYGTYFGALYATLFPSHVRRMVFDAAVNPDPAQIWYRNNL
                     DQSAAFESRWEDFREWVARHDRVYRLGGTSQAVLRSYERARAKLAAEPAGGKVGPAQLQ
                     GTFLQAGYYDDYWPSRAEALSAYLEGDPKLLIALAAPPREAAAEAENANAVYTAVECND
                     GPWPTDFRVWDRDNTRLARVAPFETWDNVWANLPCAYWRAPRQQPLDVRTGPGELPPTL
                     ILAAERDAAAPYDGALEMHRRLTGSVLVTERDA"
     gene            complement(3062..3826)
                     /locus_tag="NFX46_RS09690"
                     /old_locus_tag="NFX46_09690"
     CDS             complement(3062..3826)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983660.1"
                     /locus_tag="NFX46_RS09690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_09690"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_252547868.1"
                     /transl_table=11
                     /translation="MFWVLLLLPAWAVAGVACTRLCLAAVRSASVDANAGQSHDLTLYE
                     AAFLSGGPGRVAELTLVSMARQRRLLLAHTGWATVVDPRGRDEMERSVIGAIGPEGQSR
                     IAPVRAAAATADAVRGLADGLVGAGLAVPDGARTTVAAGVRQVRGAALTVFVLGATALL
                     MPATSDLPRVLMAAWFGLPLALTLSCLALARIEAHPYSRWASPAGQRLLAAVARQAPAG
                     DDRTYLTSIAVRGIHAAAEPELRAAFAHRDRT"
     gene            complement(3933..4715)
                     /locus_tag="NFX46_RS09695"
                     /old_locus_tag="NFX46_09695"
     CDS             complement(3933..4715)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125239.1"
                     /locus_tag="NFX46_RS09695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_09695"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_252547873.1"
                     /transl_table=11
                     /translation="MRPRPPIQGRGIFNGTGLIIAGLAVTLVALIVPVWSYEDRSGTGV
                     DVLNAETVATLYGPLSGPDRDFVTKVRLAGLWELPAGRQAQAKGTTEAVRTAGRHLVEG
                     HAFLDERVRTVAAQLGLALPNEPTDQQQDWLKTLDAAQGEDFDREFANILRLAHGRVFS
                     LVAQVRAGTQNALVRDLADDANTTVLDHIKVLEATGYVDFADLARDLAAGSTPPVTRSP
                     APPGPVADPGAVVPLTPSPDPGYPLPPAATGPPSADSP"
     misc_feature    4763..4779
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.2"
                     /tool="antismash"
     gene            complement(5001..6332)
                     /locus_tag="NFX46_RS09700"
                     /old_locus_tag="NFX46_09700"
     CDS             complement(5001..6332)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125240.1"
                     /locus_tag="NFX46_RS09700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_09700"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_252547877.1"
                     /sec_met_domain="DUF692 (E-value: 1.3e-96, bitscore: 321.8,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRLGTGIGWRPEIADAVERMPGIDWVEAVAENICPGHLPESLLR
                     LRERGVTVVPHGVSLGLGGADRPDEERLTALAERAEALGSPLVTEHIAFVRAGGPLTAT
                     PRLEAGHLLPVPRTRDALDVLCENVRIAQESLPVPLAVENIAALLAWPGEEMTEGQFLY
                     ELADRTGVRLLIDVANLHTNHVNRGEDPAKALAELPLEAIAYVHVAGGLERDGVWHDSH
                     AHPVPRPVLDILTDLTSRVAPPGVLLERDENFPEPAALERELDAIRGAVEAGARQRDTE
                     QRGASARETAGAGADGGSEPSAVAGDATRQRLGLAQAALLSSLVAGTPVPEGFDRVRLG
                     VQARALSAKRADVVAKLAPELPLILGTGYRAAFVAYAQGHPMRGGYRQDALDFAERLLL
                     GGQLAEGRARRELRRWWLDRSGPAPRPLSRLGRVGQVGRALIGR"
     gene            complement(6579..7694)
                     /locus_tag="NFX46_RS09705"
                     /old_locus_tag="NFX46_09705"
     CDS             complement(6579..7694)
                     /EC_number="1.14.19.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) delta12_cd03507"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014670087.1"
                     /locus_tag="NFX46_RS09705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_09705"
                     /product="acyl-CoA desaturase"
                     /protein_id="WP_252547882.1"
                     /sec_met_domain="delta12_cd03507 (E-value: 8.9e-20,
                     bitscore: 69.7, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVATAVADVDPGISRPGGPAGHPEVTKPPPVGRGSAYASLSREV
                     KQRGLLNRRPGYYSVKVGANLLLLAVGWTAFALIGQSWWQLVTAAFLAVMFTQTGFIGH
                     DAGHHQIATSKRVNTLLGHLHADLLIGLSYGWWIAKHNRHHAHPNHVDRDPDIADGAIA
                     FTEDQARVRRGAYAWLARQQAWLFFPMLLLEGLALHVAGVRAVFERGGTATSRRTRLVG
                     AALLTAHVGGYLAALFLVLSPVQAVCFLLVHQGLFGLYMGCSFAPNHKGMPIFGKDDRI
                     DFLRRQVLTSRNIRGGRVTDFALGGLNYQIEHHLFPSMPRPSLRHAQELVRTFCARHGV
                     AYHETGLVDSYAQVLRHLHAVGGPLRPELEY"
     misc_feature    7661..7675
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     misc_feature    complement(7733..7748)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.14"
                     /tool="antismash"
     misc_feature    7916..7929
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     gene            8455..8745
                     /locus_tag="NFX46_RS09710"
                     /old_locus_tag="NFX46_09710"
     CDS             8455..8745
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NFX46_RS09710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="NFX46_09710"
                     /product="hypothetical protein"
                     /protein_id="WP_252547885.1"
                     /transl_table=11
                     /translation="MYSQESISGHRRGRPEPTAETLSGLACLICATDYRDASAPEAVVV
                     SHRDDRQLLACRGTCARMAGGSVDGLDETPLPLDERVRRHQGPAPHDGDPS"
     gene            8802..9995
                     /locus_tag="NFX46_RS09715"
                     /old_locus_tag="NFX46_09715"
     CDS             8802..9995
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020131257.1"
                     /locus_tag="NFX46_RS09715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_09715"
                     /product="N(5)-(carboxyethyl)ornithine synthase"
                     /protein_id="WP_252547888.1"
                     /transl_table=11
                     /translation="MSLLSLGVLASSRKENEFRLPLHPAHLHRIAPDVRERIFLEQGYG
                     ERFGIADDALLPLVAGLRSREELVAECDVMLLPKPMHEDVAELREGQVLWGWPHCVQDE
                     KMTQLAIDRRLTLIAWEAMNHWTSTGAFSVHVFHKNNELAGYCSVLHALQLGGVTGSYG
                     RRLRAVVISFGATARGAVTGLGAMGVSDVTVLTQRAAAAVASPMPSVVMGHFEEEEGDP
                     SRLRAFTAAGSVPLAEYLAEFDIIVNCVLQDTDAPLTFVTEEELALFRPGTFFVDVACD
                     EGMGFGWARPTTFGDPMLTVGQDCHYYAVDHSPSHLWNSATWEISEALLPYLRKVMSGP
                     AAWEADATVGKAIEIRDGVVMNPKILSFQNRAAAYPHAAETPAPLATPATKASGRPA"
     gene            complement(10041..10751)
                     /locus_tag="NFX46_RS09720"
                     /old_locus_tag="NFX46_09720"
     CDS             complement(10041..10751)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432056.1"
                     /locus_tag="NFX46_RS09720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_09720"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_252556281.1"
                     /transl_table=11
                     /translation="MRRVSESPFQTEPHPRDEAPQFVLPLVVRLEKADPPGRTDALETA
                     ARAVLVLLSDERALGDGEWAQTVRDWQDARIRKVVRRARGAEWRRAGELPGITVTGKAA
                     EVRVFAPVPLDGWPKELAKLQVSGTDLDDPQEPSAADPGVPVLWMSPEVEMSAGKAMAQ
                     AGHGAQLAWWELSDEERSAWREAGFPLAVRTARPERWRELTASGLPLVRDAGFTEIAPG
                     SCTVVAEHPALRSR"
     gene            complement(10839..11027)
                     /locus_tag="NFX46_RS09725"
                     /old_locus_tag="NFX46_09725"
     CDS             complement(10839..11027)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125244.1"
                     /locus_tag="NFX46_RS09725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NFX46_09725"
                     /product="hypothetical protein"
                     /protein_id="WP_073493343.1"
                     /transl_table=11
                     /translation="MSDDNDDAYCLIDATRPPRAEGPPYAECVLCRKPTEYPESYKGIT
                     MCPVCEWQEAQRTACSG"
ORIGIN
        1 gctgtggtcc gtcttcaagc tgaaggacac cctccccgag gaccgcgccg gcttcgccga
       61 cgaggtccag gagctgttcg accagctcgc cgccaaggac gtgacggtcc gcggcaccta
      121 cgacgtgtcc ggtctgcgcg ccgacgccga cctcatgatc tggtggcacg ccgagaccag
      181 cgaccagctc caggaggcct acaacctctt ccgccgcacc gagctgggcc gcgcgctgga
      241 gccggtctgg tcgaacatgg cgctgcaccg ccccgccgag ttcaaccgct cgcacgtccc
      301 ggcgttcctc gccgacgaga cgccgcgcga ctacatcagc gtctacccct tcgtgcgcag
      361 ctacgactgg tacctgctgc ccgacgagga ccgtcgccgc atgctcgccg accacggcaa
      421 gatggcccgc ggctacccgg acgtccgcgc caacacggtc gcctcgttct ccctcggcga
      481 ctacgagtgg atcctcgcct tcgaggccga cgagctgtac cgcatcgtcg acctcatgcg
      541 ccacctgcgc gcctccgagg cgcgtatgca cgtccgcgag gaggtcccgt tctacacggg
      601 ccgccgcaag gacatcgccg aactggtcgc gggcctcgcc tgatcagcgc gtcggccggg
      661 cggccgccgc cttgccggcg cggtcggccg gccggggctc cgcgtgcggc gcacaggcag
      721 cgcgccgcgc cggcagccgc ccctccagca gatacgcctc caggtggccg ttgacgcaac
      781 ggttcgaccc gcccgcgatg ccgtgggtgc cggcgtcccg ctccgtcacc agcaccgagc
      841 ccgtcagccg ccggtgcatc tccagggcgc cgtcgtaggg tgcggcggcg tcccgttcgg
      901 ccgccaggat cagcgtcggc ggcagctcac ccggaccggt ccgcacgtcc aggggctgct
      961 gccggggcgc ccgccagtag gcgcacggca ggttcgccca cacgttgtcc caggtctcga
     1021 acggggccac gcgcgccagc cgcgtgttgt cccggtccca cacccggaag tccgtcggcc
     1081 acggcccgtc gttgcactcc acggccgtgt agacggcgtt cgcgttctcc gcctccgccg
     1141 cggcctcccg gggtggcgcg gccagcgcga tcagcagctt gggatcgccc tcgagatacg
     1201 ccgacagcgc ctcggcgcgg gacggccagt agtcgtcgta gtacccggcc tggaggaacg
     1261 tgccctgcaa ctgcgcgggc cccaccttcc cgccggccgg ctccgccgcc agcttcgccc
     1321 gcgcccgctc gtagctgcgc agcaccgcct gcgaggtccc gcccagccgg tacacccggt
     1381 cgtgcctcgc gacccactcc cggaagtcct cccagcggct ctcgaacgcc gccgactggt
     1441 cgaggttgtt gcggtaccag atctgcgccg ggtccgggtt caccgccgcg tcgaacacca
     1501 tccgccgcac gtgcgagggg aacagcgtcg cgtacagcgc cccgaagtag gtgccgtacg
     1561 acgcccccat gaacgtcagc cgctgctcgc cgagcgcggc ccgcaggacg tccaggtcgc
     1621 gggcgttgtt cagcgagtgg tagtgccgca gcgcgtcgcc ggcccgctgc gcgcagccac
     1681 gcgcgtacgc cttcgcccgc gcgacgcgct cccgcttgta cgtctccgag gggtgcgtcg
     1741 gggacggggc gggccccttg aagaaggtct gcggatcctc gcaggacagc ggggccgagc
     1801 ggccgacccc gcgcgggtcg tagcccacca ggtcgtaggc ggcgccgatg cgcttccact
     1861 ccgggatcat gccgatcagc gggaagtgca ggcctgaggc acccggcccg cccgggttga
     1921 agaccagcgc gccctgccgg ggcacccggc gcttgctgtt gtgcgggtcg cggtgggtgg
     1981 cccgcacccg gctgacggcc agctcgatct gcttgccgtt gggccgcgcg tagtccagcg
     2041 gcacaccgac cttgccgcat tccatactgt ccggcaggtc cggtacgtcc gtgcaggccc
     2101 cgaagtcgac cccctcagtc gctgcccgtt cggcggcgat cacgacaccg ggcacggcgt
     2161 tcggggtccc gcccgcggga acggcggaga gggtggtcag gagcagggct cccgtggccg
     2221 agtagaggac ggcagctctc atcgcgtgtc ccttcgaagc gcggcagcga cagaagggat
     2281 ggttcgtccg cctttcggcg aaggcaagca ccaccgaggg gtgtcggcgc caatgccccc
     2341 tgtgcgcccc cgggagaccc ggacgcgccg gcggcgggcg gggttcggcc tgcgtggggc
     2401 gggtcggccg ggcgcgcgcc gttcgtgggc tcacggcgcc ctggccgtgc gtcggctcgg
     2461 cacgctttcg ggggttgggc cggccgtgga ccgtggcgca ccggtcggcg ccagtcccgg
     2521 gccggcgggg ggcggtcgaa cggtggccgg gatcggccgc gggtcggtcg cgtggtcgcc
     2581 ggtgccggtg agggcccgca gtgagggtcg gctgcctttc ggccgagggt ccgcagtgag
     2641 ggtcagttgt ccttcggccg acggtccgcc gtggtggcag gggcgtcgcc tgttcggtgc
     2701 ggtgtttcgc cggtgccgtt cccgtctcgg tggcggtcgg tggtgcggca gccggtgccg
     2761 gccggggctc gccgtgcgtc agccgtcccg tcgggtgggg cccgccgtcc gccgatggtg
     2821 cgtcagcctg cgtcgtgcga gcgtcgtcgc gcgtcgtggc ggcgtcggcc tgtttcggtg
     2881 cggtgttcgc cggtgccgtt cccgtctcgg tggcggtcgg tggtgcggca gccggtgccg
     2941 gccggggctc gccgtgcgtc agccgtcccg tcgggtgggg cccgccgtcc gccgacggtg
     3001 cgtcagcctg cgtcgtacga gcgtcgccgc atcgtggcga cgacggctcg ccgtgccgcg
     3061 cctatgtcct gtcccggtgg gcgaacgccg cgcgtagttc cggttcggcc gccgcgtgga
     3121 tgccgcggac ggcgatggag gtcaggtagg tgcggtcgtc acccgcgggc gcctgccggg
     3181 ccacggcggc cagcaggcgt tgtcccgccg gggaggccca gcgggagtag gggtgcgcct
     3241 cgatgcgggc gagggccagg cagctcaggg tgagggcgag gggcagcccg aaccaggcgg
     3301 ccatgaggac gcgcggcagg tccgaggtgg ccggcatcag cagcgccgtc gcacccagca
     3361 cgaagacggt cagggctgct ccgcgcacct gccggacgcc ggccgccacg gtcgtacggg
     3421 cgccgtcggg caccgcgagg ccggcgccga ccaggccgtc ggcgagtccg cggaccgcgt
     3481 cggcggtcgc cgcggcggcc cgcaccggag cgatcctgga ctgtccctcg ggaccgatgg
     3541 ctcctatgac ggaccgttcc atctcgtcgc gcccgcgcgg gtccacgacc gtcgcccagc
     3601 cggtgtgggc gagcagcagc cgccgctgcc gggccatgga caccagggtc agttcggcga
     3661 cccggccggg gccgccggac aggaacgccg cctcgtacag ggtcaggtcg tggctctgac
     3721 ccgcgttcgc gtcgacggac gccgaacgga cggcggccag gcacagccgg gtgcacgcga
     3781 caccggcgac ggcccaggcc ggcagcagga gaaggaccca gaacatgccg cgtttctacg
     3841 ccggagacgc aggagaacgc catgccttgt tcactatccg gacccggcgt cgtgggtatg
     3901 tgacgttccg ttcgccttcg tccggccggg tgtcaggggc tgtcggccga gggcggaccg
     3961 gtcgcggccg gcgggagcgg gtaacccggg tccggtgacg gcgtgagcgg tacgaccgcg
     4021 ccggggtcgg cgacggggcc cggcggggcg ggggagcggg tgaccggcgg ggtgctgccg
     4081 gccgccaggt cccgggccag gtcggcgaag tcgacgtaac cggttgcctc caggaccttg
     4141 atgtggtcca ggacggtggt gttggcgtcg tcggcgaggt cgcggaccag cgcgttctgc
     4201 gtgccggccc gcacctgggc gacgagggag aacacccggc cgtgcgccag tcgcaggatg
     4261 ttggcgaact cgcggtcgaa gtcctcaccc tgcgccgcgt ccagcgtctt cagccagtcc
     4321 tgctgctggt cggtgggctc gttcggcagc gcgaggccga gctgcgcggc gaccgtgcgc
     4381 acccgctcgt cgaggaaggc gtgcccctcg acgaggtgcc gccccgccgt ccgtacggcc
     4441 tcggtcgtgc ccttggcctg cgcctgccgg ccggccggaa gttcccagag cccggccagc
     4501 cgcaccttcg tgacgaagtc gcggtcgggc ccggacagcg gcccgtacag ggtcgccacg
     4561 gtctccgcgt tcagtacgtc gacgccggtg cccgaccggt cctcgtacga ccagaccggg
     4621 acgatcagcg cgacgagcgt caccgccagg ccggcgatga tgagcccggt gccgttgaag
     4681 atgcctctgc cctggatcgg gggtcggggt cgcatggtgc ctcctgctca cggcaccgca
     4741 cgctcgtgct tcgggtgcgg agttggcata ttactgcggc ggcggggcca actgccgtac
     4801 gcgggcgaag cgttgcatct caggcataca gggttgaatg ggtgggccgg agcggatccc
     4861 atatgagcgg aaaccatcgg aaaacggatg ccgaggaggt ctggccgacc ccggtgttaa
     4921 ccccggcaca ctcgaacgcc gggacgacgc cacggcttca ccgcgcagtt caccgcggac
     4981 acggcccgat cagcgaccga tcagcgaccg atcagcgccc ggcccacctg tcccacgcgt
     5041 cccagccggg acagcggcct cggcgccggg cccgagcggt ccagccacca gcgccgcagc
     5101 tcgcgccggg cccgcccctc cgccaactgc ccgccgagca gcagccgctc ggcgaagtcc
     5161 agggcgtcct gccggtaacc gccgcgcatc gggtggccct gggcgtaggc gacgaacgcg
     5221 gcccggtagc ccgtgccgag gatcagcggc agctccgggg ccagtttggc caccacgtca
     5281 gcccgcttcg cggacagcgc gcgggcctgc acgccgagcc gcacccggtc gaagccctcc
     5341 gggacgggcg tgccggcgac cagtgaggac agcagggcgg cctgggcgag cccgagccgc
     5401 tgccgcgtgg cgtcaccggc gacggccgac ggctcgctcc ccccgtcggc acccgcgccc
     5461 gccgtctccc gggcggacgc cccccgctgc tccgtgtccc gctgccgtgc ccccgcctcc
     5521 accgcacctc ggatcgcgtc caactcccgc tccagtgcgg ccggttccgg gaagttctcg
     5581 tcgcgctcca gcagcacgcc gggcggggcg acccgggagg tgaggtcggt caggatgtcg
     5641 aggacggggc gcggtaccgg gtgcgcgtgg ctgtcgtgcc agacgccgtc ccgttcgagg
     5701 ccgcccgcga catggacgta cgcgatggcc tccaggggca gctcggcgag cgccttggcg
     5761 gggtcctcgc cgcggttgac gtggttggtg tgcaggttgg ccacgtcgat gagcagccgg
     5821 acgcccgtgc ggtcggccag ctcgtacagg aactgcccct ccgtcatctc ctcaccgggc
     5881 caggcgagga gcgccgcgat gttctccacg gcgagcggga cgggcagcga ctcctgagcg
     5941 atgcgcacgt tctcgcacag cacgtccagg gcgtcccggg tgcgcggcac gggcagcagg
     6001 tgaccggcct ccagccgtgg ggtggcggtc agcgggccgc ccgcccggac gaacgcgatg
     6061 tgctcggtga ccagcggcga gcccagcgcc tcggcccgct cggcgagcgc ggtcagccgc
     6121 tcctcgtcgg gccggtcggc gccgccgagg ccgagcgaga cgccgtgcgg gacgacggtg
     6181 accccacgtt cgcgcagccg gagcagggac tcggggaggt gaccggggca gatgttctcg
     6241 gccacggcct cgacccagtc gatgcccggc atgcgttcca cggcgtccgc gatctccggc
     6301 cgccagccga ttcccgtacc cagccgcgtc atggtcccct ccttcatccc cgacccccct
     6361 gcgtggtgac ggggatatga ccccgccgac cgggcccgaa ccgccgacgg tgctccttca
     6421 gagcaacatt tgaggttccg gccgcgcaca ccgcacggcc cgcccggcgc ctggaggtac
     6481 gcgcggccgg cccgcccggt tcaaagaaac ggcacagaaa gggaaggaaa cagaggaaac
     6541 gcaggaaacg ggggccgacg aaggggccgg accggcgatc agtactccag ctcggggcgc
     6601 agtgggccgc cgaccgcgtg gaggtgccgc aggacctggg cgtacgagtc gacgagcccg
     6661 gtctcgtggt aggcgacgcc gtgccgggcg cagaacgtgc gcacgagttc ctgggcatgg
     6721 cgcaggctcg ggcgcggcat ggaggggaac aggtggtgtt cgatctggta gttcagcccg
     6781 ccgagcgcga agtcggtgac ccggccgccg cggatgttgc gtgaggtcag tacctgccga
     6841 cgcaggaagt cgatcctgtc gtccttgccg aagatgggca tgcccttgtg gttgggggcg
     6901 aacgagcatc ccatgtacag cccgaacagt ccctggtgca ccaggaggaa gcacacggcc
     6961 tggacggggg acaggaccag gaacagggcg gcgagatagc cgccgacatg cgccgtcagc
     7021 agtgccgcac cgaccagcct ggtccgccgc gaggtcgcgg tgccgccgcg ctcgaacacc
     7081 gcccgtacgc cggccacgtg cagggcgagt ccttcgagga gcagcatcgg gaagaacagc
     7141 cacgcctgct gacgggccag ccaggcatac gctccgcggc ggacgcgggc ctggtcctcg
     7201 gtgaaggcga tcgcgccgtc ggcgatgtcg gggtcacggt cgacgtggtt ggggtgggcg
     7261 tggtgccggt tgtgcttggc gatccaccag ccgtagctga ggccgatcag gaggtcggcg
     7321 tgcaggtggc cgagcagggt gttgacgcgc ttggaggtgg cgatctggtg gtgtccggcg
     7381 tcatgcccga tgaacccggt ctgggtgaac atcaccgcga ggaaggcggc ggtcaccagc
     7441 tgccaccacg actgcccgat cagggcgaac gcggtccagc cgacggccag caggagcagg
     7501 ttcgccccca ccttgaccga gtagtacccg ggccgccggt tcagcagccc cctctgtttc
     7561 acctcgcggg acagcgaggc gtatgcgctg ccccggccga cgggcggggg tttggtcacc
     7621 tcggggtgac ccgccggacc gcccggacgg ctgatgccgg ggtccacatc ggccacagcg
     7681 gtcgcgactg tcacggaggt aacggaggta acggagttcg cggagttcac agtgtttcct
     7741 ttcggcgtgc acgagcgctg tctccggcgt gcgggtgggg cgccgggaac ggcgccgcga
     7801 cgtcggccct cggcacgggg cctgtctcgg aacggggaga cctcagaagg cgctgacctg
     7861 gtcggcccgc agccgctcgg ggccttggcc ggaggcggcg aggaagccgc aacgcttgtc
     7921 cgggatgaac caccgcacgg ttcgtcgagc cacgagaggt ctccttcgag atggcgagta
     7981 ggtgggagaa cgcgcactcg cgcggtcccg ggctgccggt cgccagccgt cgaagaaaac
     8041 ccccgaaacg gcgaagcgcc cgctgtcaga actccgcagg cgctgccacg tcgcaaactt
     8101 caaccacaac cactcacttc aacgtccggt gtgccgggaa cgttccccgc agctcccgga
     8161 gggcggtcgc acggggcggc cggaggcccg gagggagtac ggttgcaaca ccgagcgcac
     8221 ttcgcgtgtc gtcatccacg gcggcgtcgc cctcggggaa cggcaaggcc ggaggcctcg
     8281 cgtccggccg gaaagagccg cacgatgccg cctggcctgt tctgacgcgc ttttctcccc
     8341 atgccgttcc cgcgccccgc ggtaacggat ttcctcagtt tccggggcgt tcgagaggca
     8401 ttccggcgcc gcgcctcctg cacgtcccga accgatcaaa aggatgcagg gagcatgtat
     8461 tcacaggaat cgatctccgg tcaccgacgc ggccgtcccg aaccgaccgc cgagacgctc
     8521 tccggactgg cgtgcctgat ctgcgccacc gactaccgcg acgcgtcggc cccggaggcc
     8581 gtggtggtgt cccaccgcga cgacaggcag ctgctcgcct gccgggggac gtgcgcacgc
     8641 atggcgggcg ggtcggtcga cggcctcgac gagacgcccc tcccgctgga cgagcgggta
     8701 cgcaggcacc agggccccgc cccgcacgac ggtgaccctt cctgatccgg agacccggca
     8761 gccgtgaacc gcacgacaag aaccacatga tgaggaagag catgagcctg ttgagcctcg
     8821 gagtactcgc ctcctcccgc aaggagaacg agttccggct gcccctgcac cccgctcacc
     8881 tccaccggat cgcccccgac gtacgcgaga ggatcttcct cgaacagggg tacggcgaac
     8941 ggttcggcat cgccgacgac gccctgctgc cgctcgtggc cggactacgc tcccgcgagg
     9001 aactcgtcgc cgagtgcgac gtgatgctgc tgcccaaacc catgcacgag gacgtcgcgg
     9061 agctgcgcga gggccaggtg ctgtggggat ggccgcactg cgtgcaggac gagaagatga
     9121 cccagctcgc catcgaccga cggctgaccc tgatcgcctg ggaggccatg aaccactgga
     9181 cctccaccgg cgccttcagc gtccatgtgt tccacaagaa caacgagttg gcaggttact
     9241 gctcggtgct gcacgccctg cagctcggtg gggtgaccgg cagctacggg cgccgcctgc
     9301 gggccgtggt catcagcttc ggcgcgacgg cgcgcggagc ggtcacgggc ctgggcgcca
     9361 tgggcgtctc ggacgtcacg gtgctcaccc agcgggccgc cgcggcggtg gcgtcgccga
     9421 tgccgtcggt cgtgatgggc cacttcgagg aggaggaagg cgatccgtcg cgcctgcggg
     9481 cgttcaccgc ggccggttcc gtgccgctgg cggagtacct ggccgagttc gacatcatcg
     9541 tcaactgtgt cctccaggac accgacgcgc cgctgacctt cgtcaccgag gaggaactgg
     9601 ccctgttccg gccgggcacc ttcttcgtcg acgtcgcctg cgacgagggc atgggcttcg
     9661 gatgggcccg gccgacgacc ttcggcgacc cgatgcttac cgtcgggcag gactgccact
     9721 actacgcggt ggatcacagc ccgtcccacc tgtggaactc ggccacctgg gagatcagcg
     9781 aggcactcct gccctacctg cgcaaggtca tgagcggccc cgcggcgtgg gaggccgacg
     9841 ccacggtcgg caaggccatc gagatccgcg acggcgtcgt catgaacccg aagatcctgt
     9901 ccttccagaa ccgggcggcc gcctacccgc acgccgccga gaccccggcc ccgctcgcca
     9961 ccccggcgac gaaggcctcc ggccggccgg cctgacgatc ctgcggaggt gaccggaaac
    10021 ccggtcacct ccccgaggcc tcaccgagag cgcagcgccg ggtgctccgc gaccaccgtg
    10081 cagctacccg gcgcgatctc ggtgaacccc gcgtcccgca ccagcggcag cccgctcgcg
    10141 gtcagttcgc gccagcgctc gggccgcgcg gtgcgcacgg ccagcgggaa gcccgcctcc
    10201 cgccacgccg accgctcctc gtcggacagc tcccaccagg cgagctgcgc gccgtgcccg
    10261 gcctgcgcca tggccttgcc cgccgacatc tccacctcgg ggctcatcca cagcaccggt
    10321 acacccgggt cggccgccga cggctcctgg gggtcgtcga gatcggtccc cgacacctgg
    10381 agcttcgcca gctccttcgg ccacccgtcc aacggcaccg gcgcgaacac ccgcacctcc
    10441 gccgccttcc cggtcacggt gatcccgggc agctccccgg cccgccgcca ctcggccccg
    10501 cgagcccgcc gtacgacctt gcggatccgg gcgtcctgcc agtcgcgcac ggtctgcgcc
    10561 cactcgccgt ccccgagggc ccgctcgtcg ctcagcagca cgagcacggc ccgagcggcg
    10621 gtctcgagcg cgtcggtacg gccgggagga tcggccttct ccaaccggac gacgagggga
    10681 aggacgaact gcggtgcctc atcacgcgga tgcggctcgg tctggaacgg gctctcactc
    10741 actcggcgca gggtactgga ccgccgaacg gaccggccgg tccggccggg gccccgcggc
    10801 ccccggcggc cgcggccggt gcgcccacct cccggcgatc agcccgagca ggccgtccgc
    10861 tgggcctcct gccactcgca caccgggcac atcgtgatcc ccttgtacga ctccgggtac
    10921 tccgtcggct tccggcacag cacgcactcc gcgtacggcg gcccctcggc cctgggcggc
    10981 ctggtcgcgt cgatcagaca gtaggcgtcg tcgttgtcgt cgctcatgac tccagcgtac
    11041 gagagcggcg cgggcctcag cggcggcctt ccacggcgcc gatcagctcg gacaccttga
    11101 cgaaccggta gccccggcgg cgcagctcgg gcacgatcac gcgcaccgcc cgctcggtga
    11161 ccggggcggc gctgagcgtg cagtgcatga ccaccaccga ccccggccgc acgccctcca
    11221 gcacctgccg ggccaccgcg tccgcgtcgg tggcgaaggc gtcgccgctc accacgtccc
    11281 actgcaccgc cgtgacaccg ctgccgcgca ccgcgcgcag tgcctgtccg tc
//
