LOCUS       NZ_CP085431            20849 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Priestia megaterium strain F20 chromosome, complete genome.
ACCESSION   NZ_CP085431
VERSION     NZ_CP085431.1
KEYWORDS    RefSeq.
SOURCE      Priestia megaterium (Bacillus megaterium)
  ORGANISM  Priestia megaterium
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 5241365)
  AUTHORS   Sun,X.
  TITLE     Bacterial competition corelates with biosynthetic potential among
            closely related bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5241365)
  AUTHORS   Sun,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2021) College of Resources and Environment,
            Huazhong Agricultural University, Shizishan Street 1, Wuhan 430070,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 17-APR-2019
            Assembly Method                   :: CANU v. 1.7.11
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 328x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023824175.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 06:00:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,444
            CDSs (total)                      :: 6,214
            Genes (coding)                    :: 6,101
            CDSs (with protein)               :: 6,101
            Genes (RNA)                       :: 230
            rRNAs                             :: 17, 16, 16 (5S, 16S, 23S)
            complete rRNAs                    :: 17, 16, 16 (5S, 16S, 23S)
            tRNAs                             :: 173
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 48 of 114
            Pseudo Genes (incomplete)         :: 62 of 114
            Pseudo Genes (internal stop)      :: 19 of 114
            Pseudo Genes (multiple problems)  :: 15 of 114
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:50:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 617672
            Orig. end                         :: 638521
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085431.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20849
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="1"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..20849
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20849
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10849](+)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10849
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     gene            complement(9..227)
                     /locus_tag="LIT34_RS03285"
                     /old_locus_tag="LIT34_03290"
     CDS             complement(9..227)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016763004.1"
                     /locus_tag="LIT34_RS03285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LIT34_03290"
                     /product="spore germination protein"
                     /protein_id="WP_013055353.1"
                     /transl_table=11
                     /translation="MPAIIGPVSITSVGGGVVTFGDTFYVSPKSAAKTASGSGSLNTGN
                     FVNTNNGISGTNTLDPDLFDQNIAANA"
     gene            complement(287..718)
                     /locus_tag="LIT34_RS03290"
                     /old_locus_tag="LIT34_03295"
     CDS             complement(287..718)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081709.1"
                     /locus_tag="LIT34_RS03290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LIT34_03295"
                     /product="spore germination protein GerPE"
                     /protein_id="WP_013055354.1"
                     /transl_table=11
                     /translation="MLSRTSSVNYIEINSLSASSFCQIGDSTEIHLLNKALADRRQYPF
                     FYGREGNFDDYAIFNEPIPHGDYEPIPVQTWNEIPLIRVNRIDIKGCSFSSVIHVGSTC
                     NVYAEARIKHIRELINDEPSKEAEAGTSSSASSQASSSE"
     gene            complement(731..907)
                     /locus_tag="LIT34_RS03295"
                     /old_locus_tag="LIT34_03300"
     CDS             complement(731..907)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055355.1"
                     /locus_tag="LIT34_RS03295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LIT34_03300"
                     /product="hypothetical protein"
                     /protein_id="WP_013055355.1"
                     /transl_table=11
                     /translation="MNFNVVNRELLVGDVSITGVSSSSLFLIGDANYISLSSTFDTPAE
                     SLIIGPYVPLSLS"
     gene            complement(904..1605)
                     /gene="gerPC"
                     /locus_tag="LIT34_RS03300"
                     /old_locus_tag="LIT34_03305"
     CDS             complement(904..1605)
                     /codon_start=1
                     /gene="gerPC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461638.1"
                     /locus_tag="LIT34_RS03300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LIT34_03305"
                     /product="spore germination protein GerPC"
                     /protein_id="WP_029324684.1"
                     /transl_table=11
                     /translation="MNYIQQVHQYVQQMSSYIQQQEGRIAQLEKNVQELQQKIKELGEQ
                     PPIKIDKIEYKFEQLKIDSLDGTLNIGLNPLTPDNLEDIIINEKPEVTFSQGQQPQGEQ
                     PGQPGGPGPQNPSLKPLDRQLEKQWVSDIHETILAELNQYGFSMVQHVLDERSMMIDES
                     YYEFILEDIKKQLQQRITFYIQQVQPEEMERNFEQIKPQIIGKLYEDMKKGIDAFLTYL
                     PESLKKGGPPS"
     gene            complement(1695..1970)
                     /locus_tag="LIT34_RS03305"
                     /old_locus_tag="LIT34_03310"
     CDS             complement(1695..1970)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016763006.1"
                     /locus_tag="LIT34_RS03305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LIT34_03310"
                     /product="spore germination protein GerPB"
                     /protein_id="WP_029324687.1"
                     /transl_table=11
                     /translation="MNFYVNQSICIQSMKISAVSNSSVLQIGSVGVIKPNSNLFNTGGF
                     TGAAPTETKKGILGSVSPSTFTPGTGAGTAPAPFSQSLTPPARDND"
     gene            complement(2001..2222)
                     /locus_tag="LIT34_RS03310"
                     /old_locus_tag="LIT34_03315"
     CDS             complement(2001..2222)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879081.1"
                     /locus_tag="LIT34_RS03310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LIT34_03315"
                     /product="spore germination protein"
                     /protein_id="WP_013055358.1"
                     /transl_table=11
                     /translation="MPAIVGVVNLNSVGSASVFHIGDVYTIAPISTAKTFAGAGSFNTG
                     DGLYVVNQQSSTNTSDNDVNDQNVAGNL"
     gene            2421..3593
                     /locus_tag="LIT34_RS03315"
                     /old_locus_tag="LIT34_03320"
     CDS             2421..3593
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055359.1"
                     /locus_tag="LIT34_RS03315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LIT34_03320"
                     /product="DUF418 domain-containing protein"
                     /protein_id="WP_098325925.1"
                     /transl_table=11
                     /translation="MKRFKMNAADKEQRIEAADVLKGIALIGILLMNMPNFYSPASYYD
                     HNLLDGSRMNYVANGLVDVVVGQTGYALLAILFGFGFMKMFQRTYERQVSFTPFYVRRI
                     TALLLIGAIHALFIWHGDMIVMYSFFALIMLFFQEAKKEVLLAWGTGIFSIYAVIMIVI
                     LTAAALVNEGDTAAGIHQPIIDQALQAYGHGSFVSIFHQRLLDWYYAYNLNTVPFLFLS
                     LFPLFLLGGFMAKSEWFEEIEEYIVETKWLCLISFIVYAASSVLPYVLDHNVATSYIHD
                     TIGGTAGAIFIVTLVTLAMRSVSIQKVMQPFAFLGRMILTNYIVQSIVCTFLFYSYGLG
                     YYGHISIVEGLAIAGIIIFVQLLGSWIWMSLFSDGPLEKLIRYATYGRSR"
     gene            3672..4019
                     /locus_tag="LIT34_RS03320"
                     /old_locus_tag="LIT34_03325"
     CDS             3672..4019
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461635.1"
                     /locus_tag="LIT34_RS03320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LIT34_03325"
                     /product="DUF1516 family protein"
                     /protein_id="WP_013055360.1"
                     /transl_table=11
                     /translation="MAPLHIHVTLWTLLIISFVVSLILHRAGKAKGQKILHMVARLLYL
                     LVLVSGLHMLAAWYHFQGAALIKGIAGVLVLVGMEMVLVRTEKRKRTGVAWAVLVIALI
                     LVFYYGYVVLG"
     gene            complement(4061..4621)
                     /locus_tag="LIT34_RS03325"
                     /old_locus_tag="LIT34_03330"
     CDS             complement(4061..4621)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461634.1"
                     /locus_tag="LIT34_RS03325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LIT34_03330"
                     /product="DUF2777 domain-containing protein"
                     /protein_id="WP_013055361.1"
                     /transl_table=11
                     /translation="MNHYSKQQALNNQPRSFVIGTVECVDSQWIFFEDESDEASMLHEV
                     MDDTLELLIDTRWEKAIMVDDRQIKLHDGTYLLRNGDKLRLTKKLPYAYEQLLQSLPKE
                     TFLSFTQHLNSLKFSLYDCIYCHNTLCFLPTDGDTKGANFIIYDNEEKICAVQHLFERG
                     THHLDRFEYTVNDGERSMNLFLD"
     gene            4793..6640
                     /gene="asnB"
                     /locus_tag="LIT34_RS03330"
                     /old_locus_tag="LIT34_03335"
     CDS             4793..6640
                     /EC_number="6.3.5.4"
                     /GO_function="GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA]"
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="asnB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GATase_7"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016763010.1"
                     /locus_tag="LIT34_RS03330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LIT34_03335"
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="WP_252281759.1"
                     /sec_met_domain="GATase_7 (E-value: 2.1e-45, bitscore:
                     151.9, seeds: 307, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 3.7e-94, bitscore:
                     314.6, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MCGITGWVDLKRSLLNETATITKMADTLSKRGPDDTNVWTQQHVA
                     FGHKRLVVVDPQGGRQPMTREKNEQRYTLCYNGELYNTEDIRKELLKRGYQFQGHSDTE
                     VLLTSYIEWKESCVDHFNGIFAFSIWDEANEKLFIARDRMGVKPLFYRSYEGGIQFASE
                     LKAILAHPEVKAEVGLDGLAEVFGLGPSRSPGHGVFKEINELRPAHALTLTRNGLKIWR
                     YWNVKSGQHTDDLGETTEKVKFLVQDAIVRQLVSDVPVCTFLSGGVDSSAITAIAANAF
                     KESGKGSLHTYSIDYEDNDKFFKANEFQPNSDGTYIKLMSDTFQTNHHRCIISTEDLVQ
                     HLTEAVEVRDLPGMADVDSSLLWFCREIKQDFVVSLSGECADEIFGGYPWFHRKHDLSS
                     DTFPWMRSLDARIDLLQSSWRKKLQLEDYVRARYEETLKEVPELEGESLEDAQRRKLFY
                     LNQIWFMTTLLDRKDRMSMGASLEVRVPFADHRLVEYAWNIPWEMKMTGNKEKGILRKA
                     LEGILPHDVLYRKKSPYPKTHNPAYTKAVTVWLQSLLQDKQSPLHEFFDKQQLKHIIDT
                     GGEGFKAPWFGQLMTGPQLLAHLAQIHVWFTHNNIQIVE"
     gene            6956..8488
                     /gene="crtI"
                     /locus_tag="LIT34_RS03335"
                     /old_locus_tag="LIT34_03340"
     CDS             6956..8488
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA]"
                     /GO_process="GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="crtI"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055363.1"
                     /locus_tag="LIT34_RS03335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LIT34_03340"
                     /product="phytoene desaturase family protein"
                     /protein_id="WP_252281760.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 6.3e-07, bitscore:
                     27.2, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="DAO (E-value: 5.1e-08, bitscore: 31.1,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQKNVIVIGAGPGGLAAAMLLASHGYKVDVYEKQGYVGGRNSAIK
                     MSGYTFDMGPTFLSMPQIFEELFQAANRNAHDYMDLKRLNLMYELIFDDAQIQMTDNHK
                     KMKEEIERLYPGNGEGYERFMKDTEAKMAALLPLLQGEMDRFHHYLKPEAIRALPHLSL
                     GKTLYDVLGKYFTDEKLKLAFTFQSKYLGMSPWECPGAFSILSFMEHAYGIFHPIGGVN
                     QLSQAMAKVVEEHQGHIHLNQGVQKLWIENKRVKGIILENGERIGADDVIINGDFAHAM
                     TNLIDEGTLKKYAVKKLEKKKYSCSTFMIYLGIDKQYKDLPHHTIVFAEDYKKNVEEIT
                     KTLALSEDPSIYIQNPGVTDPTLAPEGKSALYILAPVPNNFSGINWDEKQKDFRDAVLK
                     IVEKKTGFKDLESHIEVEKIISPKQWEEDVLVYKGATFNLGHQLTQMMVLRPHNKFEEL
                     ENCWLVGGGTHPGSGLPTILESSRITTKLILKRDGQLLNEPFKTMKEMESAL"
     gene            8485..9942
                     /gene="crtI"
                     /locus_tag="LIT34_RS03340"
                     /old_locus_tag="LIT34_03345"
     CDS             8485..9942
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA]"
                     /GO_process="GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="crtI"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055364.1"
                     /locus_tag="LIT34_RS03340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LIT34_03345"
                     /product="phytoene desaturase family protein"
                     /protein_id="WP_013055364.1"
                     /transl_table=11
                     /translation="MRTAIVGGGIGGLITALLLSKRGFSVDIFEKENKLGGRLAFVEKD
                     GYKIDEGPTIVLLPLMLERILDEAGISASQYELIRCDPLYDIHFPDGTTYTKKATEKDQ
                     LEEISRLFPGEEQNYRRFMTELKKRFELGSKEFLEKTFVNKRDFWTWQNIKALKKMNAN
                     KSMRKFVSTYFHDERLVDAYSLQSLYIGGNPYSAPAIYSLVPYSEHAHGIYYVKGGYAS
                     LVPLLEKALIEQGVNIHLNKAVQELSISGDKTEGVIVDEKLYPFDQVVLNGDFPNMAKI
                     ARKKEKKYVPSSSCLLLYLGLKRKYDHHNVHQYFMGNDLAKHMEAVFKHKTIPEDPAIY
                     TFNPSVIDPSLAPEGKSVLYALVPVPSGGHIDWSNQQDFVDGIIRTLEEKGFEELRKEI
                     EWVKVRTPNDAQAFGLYEGGSFGIAPELFQSGVFRPQYQPYEYQNVFAVGASIHPGGGV
                     PIVMQGAKMLADHLIEQSSVQTVAKLS"
     gene            10001..10849
                     /locus_tag="LIT34_RS03345"
                     /old_locus_tag="LIT34_03350"
     CDS             10001..10849
                     /EC_number="2.5.1.-"
                     /GO_function="GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_phytoene_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081717.1"
                     /locus_tag="LIT34_RS03345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LIT34_03350"
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="WP_053486816.1"
                     /sec_met_domain="phytoene_synt (E-value: 2.5e-72, bitscore:
                     241.6, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PT_phytoene_like (E-value: 6.6e-91,
                     bitscore: 303.0, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMNISLDKAYNQCEEIIKENSKTFYKAFSMLPSKQRKAVWAVYAF
                     CRQVDDIVDEGCNPEIELQQFESEFARFKNGELLNESAMWVALRNVFEQYEMNVQAFDD
                     MITGQRMDLVKKEYDTLDEVKTYSYHVASTVGLMLLPILAPTTHKELEEEAVALGIAMQ
                     LTNILRDVGEDLERGRIYLPKDLMNSYGYMRMLLENRWVTSEFIEMWEHLAKEAEMYYI
                     KGLQSIHKYPVASRLPLTGAAHLYRAILDKIRKENYDVFTEKHFVTSQVKKKIMTSIST
                     G"
     misc_feature    10898..10909
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 17.89"
                     /tool="antismash"
     misc_feature    10958..10972
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 26.38"
                     /tool="antismash"
     gene            11035..11376
                     /locus_tag="LIT34_RS03350"
                     /old_locus_tag="LIT34_03355"
     CDS             11035..11376
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055366.1"
                     /locus_tag="LIT34_RS03350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LIT34_03355"
                     /product="P-II family nitrogen regulator"
                     /protein_id="WP_013055366.1"
                     /transl_table=11
                     /translation="MKKVEAVIRPEKFQALRVKLEEVGINGLTVSEVAGCGQQKGEQGL
                     FRGQSFEIKLVPKVKVEMILETEQVDEVIKIIQSICSTNQIGDGKIFVYPVEDAVRIRT
                     GEAGVEAIL"
     gene            11515..12813
                     /locus_tag="LIT34_RS03355"
                     /old_locus_tag="LIT34_03360"
     CDS             11515..12813
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461629.1"
                     /locus_tag="LIT34_RS03355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LIT34_03360"
                     /product="ammonium transporter"
                     /protein_id="WP_061859558.1"
                     /transl_table=11
                     /translation="MMWVMFGAVLVFLMHAGFAMVETGFTRSKNSLNILMKNMLTVAVS
                     SVLYFVVGFALMFGSTKGGFTGSSGFFLNGESDQMSFFVFQIVFAATCATIISGAVAER
                     MKLSSYMMLTIAMVAVIYPVVGHWVWGGGWLSKLGFVDFAGSTVVHLTGALGAAVAVIF
                     LGARLGKYGSDGKVNAIQGHNILLGALGVFILWFGWFGFNGGSTLAADPALVPGIIATT
                     LMSGSCGVLSSALYTKFRYKRIDASLTLNGALAGLVGITAGTANVSIPGAIVIGLIAGI
                     ILVEAVHFIDSKMKLDDPVGAITVHGICGIWGTLAVGLFDTANGLFYGGGVQLLGIQAL
                     GIIAVMGWTIGTVGLFLFVLTRFSSIRVSSEEEIAGLDFAEHGSSAYEFLESFVATGDG
                     NLHPEFGVGLIDRLNKVGKVSDKPHISNVNETV"
     gene            12963..14477
                     /locus_tag="LIT34_RS03360"
                     /old_locus_tag="LIT34_03365"
     CDS             12963..14477
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Alpha-amylase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1257: Alpha-glucosidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055368.1"
                     /locus_tag="LIT34_RS03360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LIT34_03365"
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="WP_107917774.1"
                     /sec_met_domain="Alpha-amylase (E-value: 1.6e-26, bitscore:
                     91.9, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKWKRTSMLLILLLLFGSSASAQDHKDIRDEVIYSLMINRFYNGS
                     EQNDRNVNYERSDAYYGGDLQGIAQKLDYIQEMGFTAVLLTPFMENDETGYHGYAVTNH
                     YKIDDHFGTLKDLQNLVKKAHERKIKIMVEFPLTISNNHTWVADKGKQTWISNESGTDN
                     EVIKALPHLNLKESAVQKYLIKNAAWWSKQADIDGYYVKDIDQAPSAFISSFSKTLKSM
                     DPSFLLIGEINGQPLKKNEQADSLGVDLLVDRTLAEATAATFSGTNRPQKMLYDKWGKR
                     SNQPLANFLDDVHSTRFTAQALKSENHPGARTKQGLSYLYTSPSVPIVFYGTEIALNGG
                     KGAENYGMMNFLANPDIIDHLKKLAELRAKSPSLRKGSFTLVSEQKGVAVYKRRYKDET
                     TFVVINNTKETSVINVPLKKVGKENELRGLITEGIIRPVDDVYNISSEPETTNVYKVVK
                     KSGFNIGYIAAVAAVYTGFGIFLYKASSKRRKEKKISQSRKKDSAS"
     gene            14535..15569
                     /locus_tag="LIT34_RS03365"
                     /old_locus_tag="LIT34_03370"
     CDS             14535..15569
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1078: LacI family
                     transcription regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055369.1"
                     /locus_tag="LIT34_RS03365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LIT34_03370"
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_013055369.1"
                     /transl_table=11
                     /translation="MGVTIKDVANLANVAPSTVSRVIADSSRISEKTKQRVRSAMKELG
                     YHPNIIARSLANQTTEAVGLVMPSSADKVFQNPFFPEVLRGISTGAHENKRAIYMSTGE
                     TEEEIFAEVVQMVQGRRVDGLILLYSKVDDQVLSYLQEQSVPFVVIGKPFKHSESITFV
                     DNDNFKAGKEATEYLIESGHERIAFIGGSLELVVTIDRLTGYDKAIQKANIPYRDEYVI
                     HEEFLQKGGKEAVCELLSLKERPTALVVADDLMALGVLNTLDEMGISVPEDISIVSFNN
                     VLIAEMARPALTSIDINIFELGYQAVRCLIKQIETPYAVAEQVRVPHKMIKRQSCKTIQ
                     MTKR"
     misc_feature    15888..15901
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     18.25"
                     /tool="antismash"
     gene            15948..17495
                     /gene="pruA"
                     /locus_tag="LIT34_RS03370"
                     /old_locus_tag="LIT34_03375"
     CDS             15948..17495
                     /EC_number="1.2.1.88"
                     /GO_function="GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA]"
                     /GO_process="GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="pruA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391658.1"
                     /locus_tag="LIT34_RS03370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LIT34_03375"
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="WP_013055370.1"
                     /sec_met_domain="Aldedh (E-value: 2.9e-155, bitscore:
                     515.6, seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVSYSHEPFTNFKEENNKKAFQEALTYVNTQLGKTYPLVIDGEK
                     VETEEKITSVNPANIKEVIGYVSTVNKDLAEQAMQSALKAFETWKKWKVEHRADILFRA
                     AAIIRRRKHEFSSYLVKEAGKPWNEADADTAEAIDFLEYYARQVLTLKDGSPVQSRDGE
                     YNQYNYIPLGVGIIISPFNFPLAIMAGTAVAAIVTGNTILLKPADATPVVAAKFVEVME
                     EAGLPKGVLNFIPGATPEIGDYLVEHPKTRFVSFTGSRAVGCRIYERAAKVQPGQKWLK
                     RVIAEMGGKDTVVVDKDADLDLAASSIVYSAFGFAGQKCSAGSRAVVHQDVYDEVLEKA
                     VALTKTLTVGTPEEVSTYMGPVIHEASYNKVLSYIEIGKEEGRLVAGGEADNSTGYFIQ
                     PTIFADVDEKARLMQEEIFGPVVAFSKARDFDHMMEIANNTEYALTGALLSNNREHIER
                     AREEFHVGNLYFNRGCTGAIVGYQPFGGFNMSGTDSKAGGPDYLVLHMQAKTTSETF"
     gene            17519..18724
                     /locus_tag="LIT34_RS03375"
                     /old_locus_tag="LIT34_03380"
     CDS             17519..18724
                     /GO_function="GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-350). E-value: 2.9e-120.
                     Score: 393.3. Matches aSDomain:
                     nrpspksdomains_LIT34_RS03375_Aminotran_3.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461624.1"
                     /locus_tag="LIT34_RS03375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LIT34_03380"
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="WP_013055371.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.1e-136, bitscore:
                     454.0, seeds: 13, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_1_2 (E-value: 2e-05, bitscore:
                     22.2, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTETQKTVKIIEQTEKFGAHNYHPLPIVIEKAEGVWVHDPENNK
                     YMDMLSAYSAVNQGHRHPKIIEALKRQADKVTLTSRAFHNDQLGPWYEKICKLTNKNMA
                     LPMNTGAEAVETAIKAARRWAYDVKGVAENQAQIIACVGNFHGRTMTAVSLSSEAEYQR
                     GFGPMLPGLQTIPYGDLEALKAAITPNTAAFLIEPIQGEAGIVLPPEGFLKAAADLCKE
                     HNVLFIADEIQVGLARTGKMFACDWEGVEPDMLILGKALGGGVFPISCVVANEDILSVF
                     NPGSHGSTFGGNPLACAVSLAALEVIEDEELPARSLELGSYFKQKLQSLSNPVIKEVRG
                     RGLFIGVELHEEARPYCEKLKDQGLLCKETHSTVIRFAPPLTISKEELDWAIERIEKVF
                     AE"
     aSDomain        17603..18568
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LIT34_RS03375_Aminotran_3.1"
                     /evalue="2.90E-120"
                     /label="LIT34_RS03375_Aminotran_3.1"
                     /locus_tag="LIT34_RS03375"
                     /protein_end="350"
                     /protein_start="28"
                     /score="393.3"
                     /tool="antismash"
                     /translation="VIEKAEGVWVHDPENNKYMDMLSAYSAVNQGHRHPKIIEALKRQA
                     DKVTLTSRAFHNDQLGPWYEKICKLTNKNMALPMNTGAEAVETAIKAARRWAYDVKGVA
                     ENQAQIIACVGNFHGRTMTAVSLSSEAEYQRGFGPMLPGLQTIPYGDLEALKAAITPNT
                     AAFLIEPIQGEAGIVLPPEGFLKAAADLCKEHNVLFIADEIQVGLARTGKMFACDWEGV
                     EPDMLILGKALGGGVFPISCVVANEDILSVFNPGSHGSTFGGNPLACAVSLAALEVIED
                     EELPARSLELGSYFKQKLQSLSNPVIKEVRGRGLFIGVELH"
     gene            18828..20507
                     /locus_tag="LIT34_RS03380"
                     /old_locus_tag="LIT34_03385"
     CDS             18828..20507
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016763016.1"
                     /locus_tag="LIT34_RS03380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LIT34_03385"
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="WP_053486820.1"
                     /transl_table=11
                     /translation="MYIQLSQLSMEEQVECLTKALVKIKSINGTSGEVKIADFIKNTLQ
                     SFPYFKENPLHVWEQKINGDTLGRKNVFAFIQGSKKNAQTMIYHAHLDTVGIEDFGPLK
                     DIALDPEELQRYFSMHNINEDVQRDARSGEWMFGRGAVDMQSGIAVHLANVLHFTKHPN
                     KLEGNVLFMVNPDEESQHAGIISAVSELNRLKKEKNLSYVAAINTDFITPLYDGDSTRY
                     IYTGAAGKLLPCFYIYGREVHVGDTLAGIDPNLIASEITGSIHNNINLAENIEGELVLP
                     PSCLYQRDNKEAYNVQTAVSSHLYFNYFIYERTAKEVMSQLIGLSIEACEKVEKKLSDY
                     YELFVRRTNLPKRNLSWKVDVVSLEDYLEELDQKGINAQACIERAFEQYAHLELRTRCF
                     KVIEELQKLDPHQSPRVIVFFAPPYLPHNHLKKDESRDQQLLYPLQAAVEKVGKQTGET
                     FQFKKFFPYLADGSFLSLHETDEEIDAFTRNFPGWGIAGAIPFREIRELNIPSINIGVY
                     GKDGHKWTERVYKPYSFGVLPKLIQETTVQMLKSRHACTNHI"
     misc_feature    complement(20532..20545)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.99"
                     /tool="antismash"
ORIGIN
        1 gggttttttt aagcatttgc tgctatattt tgatcgaata aatctggatc taacgtattt
       61 gttccggaaa tgccgttatt tgtattaaca aagttacctg tatttaaaga accagagcct
      121 gacgctgttt tagcagcgct ttttggagaa acataaaacg tatctccaaa cgttacgaca
      181 ccgcctccga cgctcgtgat actaacgggt ccaattattg ctggcatgag attcacaccc
      241 ttttttcgtg atattaattt agtatatgag ccgcttttta aaaggttcac tcactgctgc
      301 tagcttgtga agaagcacta cttgatgttc ctgcttcagc ttcctttgaa ggttcatcgt
      361 tgatcagttc gcgaatatgc ttaatgcgag cttctgcata aacattgcac gttgagccaa
      421 catgaataac tgatgagaat gagcatccct ttatatcaat tctgttaaca cggatcaaag
      481 gtatttcatt ccatgtttga acaggaatag gttcgtaatc gccatgagga ataggctcat
      541 taaagatagc gtaatcatca aagtttcctt ctcgaccgta gaaaaacgga tactgtctcc
      601 gatcagctaa agctttatta agtagatgaa tttctgttga atctccaatt tggcaaaagg
      661 agctcgccga caaggaatta atttctatat aattaacaga agatgtgcgt gaaagcatag
      721 gtaagctcct ttatgataac gataatggaa cataagggcc gataatcaaa gattcagccg
      781 gcgtatcaaa tgttgaggat agagagatgt aattggcatc accaattaaa aaaagagaag
      841 aagaagatac gcctgtaata ctaacgtctc caacaagcaa ttcgcggttt acaacgttaa
      901 agttcatgaa gggggtccac ctttctttaa agattcaggt aaatacgtta aaaatgcgtc
      961 aattcctttt ttcatatctt cataaagttt accgataatt tgaggtttga tttgttcaaa
     1021 atttcgttcc atttcttccg gctgaacttg ctgtatataa aaagtaatcc gctgctgcag
     1081 ctgcttttta atatcttcta aaataaactc gtagtacgat tcatcaatca tcatcgagcg
     1141 ctcatcaagc acatgttgaa ccatagaaaa tccatattga ttaagttcag ctaaaatcgt
     1201 ttcgtggata tcagagaccc actgtttttc tagctggcgg tccaatggtt ttaagctagg
     1261 attttgcggt ccaggacctc cgggttgtcc cggctgctca ccttgaggct gctgcccttg
     1321 cgaaaacgtt acttctggtt tctcattgat aatgatatct tctaaattat caggcgtaag
     1381 aggatttaat ccaatattta atgttccatc taatgaatca atttttaact gttcaaattt
     1441 atattcaatt ttatcaattt taataggtgg ctgttcacct aactctttta ttttttgctg
     1501 aagttcttgt acgttttttt caagctgagc aatgcgtcct tcttgctgtt gaatatacga
     1561 agacatttgc tgaacatact gatgtacttg ctgaatatag ttcatgttat ccaccttcaa
     1621 tcacctcgat tcccttatct tctgataaag aaaatagtta tatatataca tatggtgatg
     1681 aaggttgtaa catgtcagtc gttgtctcga gctggaggag ttaacgactg tgaaaaagga
     1741 gctggggcag tgcctgcgcc ggttccggga gtaaaggtag aaggagaaac agatccaaga
     1801 atgccttttt ttgtttcagt aggtgcggcg cctgtaaatc ctcctgtatt aaagagattt
     1861 gagtttggtt taatgacacc gacgcttcca atttgaagta ccgaagagtt acttacagca
     1921 ctgattttca tactttgaat acagatactt tgattcacat aaaagttcat aagcatcctc
     1981 ctgtatgagc ttcatttcta ttatagattt ccagcgacat tttgatcatt tacgtcattg
     2041 tctgacgtat tggttgagct ctgctgattc acaacatata atccgtcacc tgtattaaac
     2101 gaacctgcgc cagcaaatgt tttagcagtg gaaatagggg ctattgtata aacatctcca
     2161 atatgaaaga cgctggctga accaacactg ttaagattaa caacaccaac aatggcaggc
     2221 ataggcaaac acccttttaa tttagtctag taacagcata tgaagcctag acaaaaaggt
     2281 gattaatgct ttttctttat ctatatgccg agagtaagca aaacattaaa gagaggaatg
     2341 gaaatagtaa acattgaaga aaaacttttg cgttcgatat aatgtgaaag caataatgaa
     2401 ataatgtaag gtgtgaaaga atgaaacgtt tcaaaatgaa cgcagctgat aaagaacagc
     2461 gtattgaggc agcggatgta ttaaaaggaa tcgcgcttat tggtattttg ctcatgaaca
     2521 tgccgaattt ttattcacct gcttcctatt acgaccataa tttgttggac ggttcaagaa
     2581 tgaattatgt tgctaatgga ttagttgatg tagttgtagg gcagacaggc tacgcgctgc
     2641 tcgctatttt gtttggattt ggttttatga aaatgttcca gcgtacatac gaaaggcaag
     2701 taagctttac tcctttttat gtacgaagaa tcacggcttt gcttctcatt ggggctattc
     2761 atgctttatt tatttggcac ggtgatatga ttgtgatgta cagcttcttt gctttgatca
     2821 tgctattttt tcaagaagca aagaaagaag tgttattagc gtggggaacc ggaatattta
     2881 gcatttatgc tgtgattatg attgttattt taacagcagc ggctttggtt aatgaaggag
     2941 atacagcagc gggaattcac cagcctatta ttgaccaagc gcttcaagca tatggacacg
     3001 gaagttttgt gtcaatcttt catcagcgct tgcttgattg gtactacgcg tataatttaa
     3061 atacggttcc ttttctgttt ttatcgctat ttccactgtt tttattaggc ggttttatgg
     3121 caaaaagcga gtggttcgaa gaaatagaag aatatatagt ggagacaaag tggctttgtc
     3181 ttatttcatt catcgtgtat gcagcatcat ctgtattgcc gtatgtttta gatcacaatg
     3241 tagcgacatc ttatattcac gatacaatcg gaggaacggc aggagcaatt tttattgtaa
     3301 cgttggtcac attagctatg cgaagtgtat ctattcaaaa agtcatgcag ccatttgcct
     3361 ttttggggag aatgatttta actaattata ttgtccagtc cattgtatgt acatttcttt
     3421 tttactcata tgggttagga tactatggac atatttcaat tgtagaaggc ttggctattg
     3481 cggggattat catttttgtt caactgctgg gaagctggat atggatgagc ctattttcag
     3541 atggacccct tgaaaaactc attcggtatg ctacttatgg acgcagccgc taaaaatcct
     3601 tttccttcct tatatcattg gtgaaggaaa ggttttcatg atatgatatt ttcagaaagg
     3661 acgtgagata aatggctcca ctacatattc atgtaacgct ttggacatta ttaattatct
     3721 cgtttgtggt ttcgttgatt ttacatcgag caggtaaagc taaaggtcaa aaaatcctac
     3781 atatggtggc gagactccta tacttattag tattagtatc aggtctgcat atgttagcag
     3841 cttggtacca ttttcaaggc gcagcgctca ttaaagggat tgcaggcgtg ttagtgcttg
     3901 tgggaatgga aatggtactt gttcgtactg aaaaaagaaa gcgtacaggc gttgcttggg
     3961 cagttcttgt aattgcgctt atcttagtat tctattacgg atatgtcgta ctaggataag
     4021 aaaaaagcta tcttggataa gatagctttt ttcttttgct ttaatctaaa aaaagattca
     4081 ttgaacgttc tccatcatta acggtatatt caaatcgatc taggtgatgc gtgcctcgtt
     4141 cgaataaatg ctgaacggca caaatttttt cctcgttgtc ataaatgata aaattagcac
     4201 cttttgtatc accgtctgtc ggtaaaaaac ataatgtgtt gtgacagtaa atacaatcat
     4261 aaagagagaa ttttaatgaa tttaagtgct gagtgaatga taaaaaggtc tctttcggta
     4321 gtgattgaag aagctgttcg tatgcataag gtagcttttt agtcagtctt agcttatccc
     4381 catttcgcaa taaatacgtt ccatcgtgaa gctttatttg acggtcatca accataatgg
     4441 ctttttccca gcgtgtatca ataagaagtt ctaatgtatc atccattact tcatggagca
     4501 tggaagcttc gtcactttca tcttcaaaga aaatccactg tgaatcaaca cattcaaccg
     4561 taccaatcac gaaggaacgc ggctgattgt tcagagcttg ttgtttagaa taatgattca
     4621 taatcaaacc tccgtgtgaa atttatccat atttttttat tgcctaaaaa agctctcttt
     4681 ataactcgaa agtgaaaata aaatataagg acaattcaac tgtaactatt gcgtgcatac
     4741 aatggcatta tttcattcat aaacaagagc gtgaaagaaa ggatgataaa cgatgtgtgg
     4801 tataacgggt tgggtcgact taaagcgatc gcttttaaat gaaacagcaa caataacaaa
     4861 aatggctgac actttatcga aaagaggtcc tgatgacacc aacgtatgga cacagcagca
     4921 tgttgctttt gggcacaaac gcttagttgt agtagatcct caaggcggaa gacagccgat
     4981 gacaagagaa aaaaatgaac agcgctatac gctttgctat aatggagagc tttacaacac
     5041 ggaagatatt cggaaagaat tactaaaaag aggttatcaa tttcagggac attcagatac
     5101 ggaagtactt ctaacatctt atatagaatg gaaagaatca tgcgtagatc attttaacgg
     5161 tatctttgct ttttcaatct gggacgaagc caatgaaaag ttatttatag cacgcgacag
     5221 aatgggagtg aagcccttat tctatcgatc ttatgaaggt ggaattcaat ttgcatcgga
     5281 attaaaagcg atattagctc atcctgaggt gaaagccgaa gtaggattag atggattagc
     5341 agaagtgttt ggcttaggac cttctcgttc tcccggccac ggcgtattta aagaaataaa
     5401 tgaattgcgc ccggctcacg ccctcacgct tacgcgtaac gggttgaaga tttggcgata
     5461 ctggaacgta aaaagtgggc agcatacaga tgatttaggt gaaactacag aaaaagttaa
     5521 atttttagtg caagacgcga ttgtaagaca gctagtgtcc gatgtcccgg tttgtacatt
     5581 tttatcaggt ggagtagatt caagcgccat tacagcaatt gctgcaaatg cgttcaaaga
     5641 aagcggcaag ggctctcttc atacgtattc tatcgattat gaagacaatg ataaattttt
     5701 taaagcaaat gagtttcagc caaattctga tggaacttat atcaagctca tgtcggatac
     5761 gtttcaaaca aatcatcacc gctgcattat ttcaacggag gaccttgtgc agcatttaac
     5821 agaggctgta gaggtgagag accttcctgg aatggctgat gtagattcat cgctgctttg
     5881 gttttgccgt gaaatcaaac aagattttgt tgtgagctta tcaggagaat gtgcggatga
     5941 aatttttgga ggatatccgt ggtttcaccg aaaacatgat ttgtcttcgg ataccttccc
     6001 atggatgcgt tcattagatg ctcgaattga tttgctgcaa agttcatgga gaaagaaatt
     6061 acagttagaa gattatgtgc gtgcaagata tgaagaaaca ttaaaggaag tgcctgaatt
     6121 agaaggtgaa tcgttagaag atgcgcagcg aagaaagctg ttttatttaa atcagatttg
     6181 gtttatgaca actttattag atcgtaaaga tcgtatgagc atgggcgcga gcttagaagt
     6241 acgcgtacct tttgcagatc atcgccttgt ggaatatgca tggaatattc cgtgggagat
     6301 gaaaatgacg ggcaataaag agaaggggat tttacgaaaa gcgcttgaag gaattttgcc
     6361 tcatgatgtt ctttatcgca agaaaagccc ttatcctaaa acgcataacc cagcttatac
     6421 aaaagctgta actgtatggc ttcaatctct tttacaagat aaacagtcac ctcttcatga
     6481 attttttgat aagcagcagt taaagcatat tattgataca ggcggagaag gatttaaagc
     6541 accttggttt ggtcaattaa tgacaggacc tcagctgcta gctcatctag ctcaaattca
     6601 cgtctggttt actcacaata atattcaaat tgttgaatga caggaccctc tcagtacaga
     6661 gggttttttt tatgaaataa gtcatctgtt tctctttttt gtacgtttaa ctagagaaga
     6721 agaccgcagt ctatttttgt ataactttac atagatataa aataaaagct tgaaagcacc
     6781 gaataaaggg aaagtgtact gaaaccctgg aaaaatggta caaacgtcat gttttaatgg
     6841 tttagttatt ggtaatttta tgattcgaat aaagacaagc agtttatcgt tgtatttaat
     6901 aataaagatt tgtataatgt ttgtataact attgtatagg attaggagtg tatgtatgca
     6961 aaaaaacgta atcgtgattg gagcaggtcc aggaggacta gcggcagcga tgctgttagc
     7021 aagtcatggc tataaagtag atgtatatga aaagcaagga tacgtgggcg ggcgaaattc
     7081 agcgattaaa atgtcaggtt atacgtttga tatgggtcct acatttttga gcatgccaca
     7141 gatttttgaa gaattatttc aagctgctaa tcgcaatgcg catgattata tggacttaaa
     7201 acgtctaaat ctcatgtatg agcttatttt cgatgatgcg cagattcaga tgacggataa
     7261 ccataaaaag atgaaagaag aaatcgaacg tttataccca ggaaacggag aaggctatga
     7321 gcgctttatg aaagacaccg aagcaaagat ggcggctttg ctgcctttat tgcagggcga
     7381 gatggatcgt tttcatcact atttaaagcc tgaagcaatt cgagcactgc cgcatttatc
     7441 cttaggtaaa acgctctatg atgtacttgg caagtatttc actgatgaga aattgaaatt
     7501 agcttttact tttcaatcta agtacttggg aatgtcccct tgggaatgtc caggagcctt
     7561 ttctatttta tcgtttatgg aacacgctta cggtatcttc catccaatag gaggagtaaa
     7621 ccaactatct caagcaatgg caaaagtagt ggaagaacat caagggcaca tccacttaaa
     7681 ccaaggtgtc caaaaactat ggattgaaaa taagagagta aaaggaatta ttctagaaaa
     7741 tggagagcga ataggggcag acgacgtcat tatcaatgga gattttgctc atgccatgac
     7801 gaatttaata gatgaaggta cccttaaaaa atatgcagtt aaaaaacttg aaaagaaaaa
     7861 atactcttgt tctactttca tgatttatct aggcatagac aaacagtaca aagatctgcc
     7921 tcatcacacg attgtgttcg ctgaagatta taaaaaaaat gtagaagaga tcacaaaaac
     7981 gctagctctt tcagaggacc cttctatcta catccaaaac ccaggtgtaa cagacccaac
     8041 tttagcacca gaaggaaagt ctgctcttta tattttagcg ccggttccaa ataatttcag
     8101 cggtataaac tgggatgaaa aacaaaaaga ttttcgggat gcagtattaa aaattgtaga
     8161 aaagaaaacg ggtttcaaag atttagagag tcatattgaa gttgaaaaaa tcatttcacc
     8221 aaagcagtgg gaagaagacg tgctggtcta taaaggagcg acatttaacc ttggacatca
     8281 gctcactcaa atgatggtgc tgcgtccgca taataaattt gaagaattag aaaactgctg
     8341 gctagttgga ggaggaactc atccaggaag cggtctacca actattctcg aatcatctcg
     8401 cattacgaca aagctcatct taaaaagaga cggacaatta ctgaatgagc cgtttaaaac
     8461 catgaaggaa atggagagtg cattatgaga acagcaattg ttggaggcgg cataggcggt
     8521 ttaattaccg ctttgttatt aagcaaacga ggattttctg ttgatatttt cgaaaaagaa
     8581 aacaagcttg gcggccggtt ggcgtttgta gaaaaagacg gttataaaat tgatgaaggt
     8641 cccacgattg tcttactgcc tttaatgctt gaacgtattt tagatgaagc aggcatttct
     8701 gcttctcaat atgaactaat acgctgtgat ccactttatg atattcattt tccagacggt
     8761 acgacctata caaaaaaagc gactgaaaaa gatcagttag aggagattag ccgtttgttt
     8821 ccaggcgaag agcaaaacta ccggcgtttt atgactgaat taaaaaagcg atttgaatta
     8881 ggaagtaagg aatttttgga aaaaacattt gtaaataaac gagatttttg gacatggcaa
     8941 aacatcaagg cattaaaaaa aatgaatgca aataaatcaa tgagaaaatt tgtttcaacg
     9001 tatttccatg atgaacgtct agtagatgcc tattccctgc aatctctcta tattggagga
     9061 aatccttatt cggcgccagc tatttattcc cttgttcctt acagtgagca tgctcatggc
     9121 atctattacg taaaaggcgg atacgcaagt cttgtgccac ttcttgaaaa ggcgctgata
     9181 gagcaaggtg taaacattca tttgaataag gcggttcaag agctttctat atcaggagac
     9241 aagacggaag gggttattgt tgacgaaaaa ctttatccgt ttgatcaagt tgttttaaat
     9301 ggcgactttc ctaatatggc gaaaattgct cgaaaaaaag aaaagaagta tgtgccttcg
     9361 tccagctgtc tgctgcttta tcttggtttg aagagaaaat atgatcatca caatgtgcat
     9421 caatacttta tgggcaatga tttagctaaa catatggaag cagtgtttaa gcataaaacg
     9481 attcctgaag atcccgctat ttatacattt aatccctctg ttattgatcc atcgttagcc
     9541 cctgaaggga aaagtgtgct atatgcactt gtaccggttc catcaggtgg ccacattgat
     9601 tggagcaatc agcaagattt tgttgatgga atcattcgaa ctttagaaga aaaagggttt
     9661 gaagagcttc gtaaagaaat agagtgggta aaagtacgca cgccaaatga tgctcaagca
     9721 tttggacttt acgaaggagg aagctttggt attgctcctg agctgtttca atcgggcgta
     9781 ttcagacccc agtaccagcc gtacgaatat caaaatgttt ttgcggtcgg agcatctatt
     9841 catccaggcg gaggcgtgcc aattgttatg caaggagcaa aaatgttagc tgatcatctt
     9901 attgaacaat catctgttca aacggttgca aagctttctt aagatcttgt aagccataag
     9961 gaaatgatgg tttatccttt aatagagaaa ggggtcctat atgatgaaca tttcgttaga
    10021 caaagcgtac aatcagtgtg aagaaattat taaagaaaac tcaaaaacgt tttacaaagc
    10081 tttttccatg ctgccgtcaa aacaaagaaa ggcagtatgg gcagtatatg cattttgccg
    10141 tcaagtcgat gatattgtag atgaaggatg caaccctgaa atagagctgc agcaattcga
    10201 atcagaattt gcccgattta aaaatggaga actattaaac gaaagcgcca tgtgggtagc
    10261 tctgcgcaat gtatttgaac aatatgagat gaatgttcaa gcgtttgatg atatgattac
    10321 cggccagaga atggatttag taaaaaaaga atacgatacg cttgatgaag tgaaaacata
    10381 ctcttatcac gtagcgagta cggtagggtt aatgctttta ccgattttag ctccaactac
    10441 tcataaagaa ttagaagaag aagcagttgc attaggcatt gcgatgcagc tgaccaacat
    10501 tttaagagac gttggggaag acttagagcg agggcgcatt tatttaccga aagaccttat
    10561 gaattcttac ggatatatgc gcatgctatt agagaaccgg tgggtcactt ctgaattcat
    10621 agagatgtgg gagcacctgg ccaaagaagc ggaaatgtat tatataaaag gacttcagtc
    10681 gattcataaa tatccggtgg cttctcgttt gcctcttaca ggagcggcgc atttataccg
    10741 agcgattctt gataaaattc gaaaagaaaa ttatgacgta tttacagaaa agcactttgt
    10801 cacaagtcaa gtgaaaaaga aaattatgac atctatttct acggggtaac gcaaaggaat
    10861 tacttttcct ttgcgttact tttttttgaa aagatatgaa agcgttaact attatgtaaa
    10921 taagaaaaaa gtctcatttt tatcacatat taacttgaat attcagaaaa ttacctatat
    10981 gataatatac ataacattaa atgttaataa aactaacatg gaagaggggt tacgatgaaa
    11041 aaagtagaag ccgtcattcg tccagaaaaa tttcaagcac tgcgcgtaaa attagaggaa
    11101 gtaggaataa acggattaac ggtctcagaa gtagcaggat gcggtcagca aaaaggagag
    11161 cagggactgt tcaggggaca aagttttgaa atcaaactag tacctaaagt caaggtagaa
    11221 atgattttag aaacagaaca agttgatgaa gtcattaaga tcattcaatc aatttgttct
    11281 acgaatcaaa taggcgacgg aaagattttt gtatatccgg tagaggatgc tgtacgaatt
    11341 agaacaggag aagcaggtgt agaggctatt ttataaaaag agagtgttat tttaatagag
    11401 ggggagttaa gcgtcgaaaa agaaaattag tgcggctttg tttattagtt tgttggcagc
    11461 acacgcgtta atgcggctga cccaactgtt gaatcagtca gcgcatcaat tgacatgatg
    11521 tgggtaatgt ttggggcagt acttgtgttt ttaatgcacg cagggtttgc gatggttgaa
    11581 acaggcttca ctcgttcgaa aaattcgctt aatatcttaa tgaaaaatat gctaacggta
    11641 gctgtgtctt ccgtcttgta ttttgtggtt ggttttgcgc ttatgtttgg aagtactaaa
    11701 ggaggattta ccggttcgag cggtttcttt ttaaatggag aaagcgatca aatgagcttt
    11761 tttgtctttc aaattgtttt cgcagcaacg tgcgctacga ttatttcagg agcggtagca
    11821 gaacgtatga aattatcgag ctatatgatg ctcacaattg ctatggtagc tgttatttat
    11881 ccagttgtag gtcactgggt gtggggagga ggatggttgt ctaaactagg cttcgtagat
    11941 tttgcagggt cgacagttgt tcatttgact ggagctcttg gtgcggctgt agcagttatt
    12001 tttctaggag cacgtcttgg aaagtatgga agcgatggaa aagtcaatgc tattcaagga
    12061 cataatattc tacttggcgc attaggtgta tttatcctct ggtttggatg gtttggattt
    12121 aatggaggaa gtacattagc agcggatcct gcccttgtac caggaatcat cgcaactaca
    12181 ttaatgtctg ggtcatgcgg agtcctgtct tctgctttat atacaaagtt tcgctacaaa
    12241 cgtattgacg ctagcttaac attaaacggt gccttagctg gacttgtagg gattacagct
    12301 ggaacagcaa acgtatcgat tccaggtgcg atcgtcattg ggctaattgc aggaattatt
    12361 ctagtagaag ctgtgcattt tatcgatagc aaaatgaagt tagatgatcc agttggtgct
    12421 attactgttc atggaatttg cggtatttgg gggacgttag cagtgggact gtttgataca
    12481 gctaatggtt tattttatgg cggtggcgta cagctattag gtattcaagc tcttggaatt
    12541 atagctgtga tgggctggac aattggaacg gttgggttat tcttatttgt tctcacgcgt
    12601 ttttcttcta ttcgagtatc aagcgaagaa gaaattgctg gtttagattt tgctgaacac
    12661 ggttcatccg catacgaatt tcttgaaagt ttcgtagcga caggagatgg taaccttcat
    12721 cctgaatttg gtgttggatt gattgatcga ttaaataaag ttggtaaagt atcggataaa
    12781 cctcatatat cgaacgtaaa tgagacggta tagcagtata tagatggaaa aaagtgatta
    12841 aaagtaaaaa cttttaatca ctttttttgt ttttatcaac ttatttaaca ataatgtaag
    12901 cgttaacgtt ataatggaaa caaaagtgag tttgaaacag ttcgtctata ggaggggaat
    12961 agatgaaatg gaaaaggaca agtatgctac ttatactatt attgcttttt ggaagcagtg
    13021 cctcagcgca agatcataaa gatatacgtg atgaggtaat atactcattg atgatcaacc
    13081 gtttttataa cggaagtgaa cagaatgatc gaaatgttaa ctatgaacgc tcagatgcct
    13141 attatggagg cgatttacag ggaatagcac aaaagttaga ctatattcaa gagatgggat
    13201 ttaccgctgt tttattaact ccttttatgg aaaatgatga gacaggctat catggatatg
    13261 cggtgaccaa ccattataaa atagacgatc attttggaac gttaaaagac ttacaaaatc
    13321 ttgtgaagaa agcacatgag cgcaagataa aaattatggt cgaatttcct ttgacaatca
    13381 gtaacaatca tacctgggta gcagataaag gaaaacaaac atggataagt aatgaatccg
    13441 gtacagataa cgaagtaatt aaagcattgc ctcaccttaa tttaaaagaa agtgctgtgc
    13501 agaaatacct tattaaaaat gctgcttggt ggagcaaaca agcagatatt gacggatatt
    13561 acgttaaaga tatcgatcaa gctccttctg catttatttc atccttttct aagacgctaa
    13621 aaagcatgga tccttctttt ctattaatag gagaaataaa cgggcaacca ttaaaaaaaa
    13681 atgagcaggc tgattcgtta ggagtagatt tgcttgtaga tagaacgcta gccgaagcca
    13741 ctgctgctac gtttagcggt actaatcgtc cgcagaaaat gttgtatgac aagtggggga
    13801 aaagaagtaa tcagccttta gccaattttt tagatgatgt tcatagcaca cgttttacag
    13861 cacaagcatt aaagagtgaa aatcatcctg gagcccgaac aaagcaaggg ctttcctacc
    13921 tttatacttc gccgagtgta ccaatcgttt tttatggaac ggaaattgcg ctgaatgggg
    13981 gaaagggagc agaaaattat ggcatgatga attttttagc taatcccgat attatagatc
    14041 atttaaaaaa actggctgaa cttagagcaa agtcgccttc tcttcgaaaa ggaagcttta
    14101 cccttgtgtc tgagcaaaaa ggtgtggctg tatacaagag aaggtataag gacgaaacga
    14161 cgtttgttgt gattaataat acaaaggaaa cgtcggtcat taatgtcccg cttaaaaaag
    14221 ttggaaaaga aaatgaacta agagggttaa taacggaagg aattatacga cccgttgacg
    14281 atgtatacaa catttcatcg gagcctgaga ccacaaatgt atataaagta gtgaaaaagt
    14341 cagggtttaa tattggatat atcgcagcgg ttgctgctgt ttatacggga tttggcatct
    14401 ttttgtacaa agcatcttca aaaagaagaa aagaaaagaa aatttctcaa tctcgtaaaa
    14461 aggacagcgc gtcgtagttg tcaaattata ttggataagt attaatgaag aaaacataat
    14521 taagaggtgg aattatgggt gtaacaataa aagatgtggc aaatttagca aatgtagccc
    14581 cttcgacggt atctcgcgtc attgcagaca gctcaagaat tagtgaaaaa acaaaacagc
    14641 gcgtgcgatc ggctatgaaa gaacttggtt atcacccaaa tattattgcg cgaagtctag
    14701 caaatcaaac gacagaggca gtcggcctag tgatgccgag ctcagcagac aaggtgtttc
    14761 aaaatccatt ctttcccgaa gtgctaagag gaatcagcac cggagctcac gaaaacaaaa
    14821 gagcgattta tatgtccact ggagaaacgg aagaagagat ttttgcagaa gttgttcaaa
    14881 tggtacaggg gagacgagtg gatggattaa ttttgctgta ctccaaagtt gatgatcaag
    14941 tactttctta tttgcaagaa cagagcgtgc cgtttgtagt tattggaaag ccgtttaaac
    15001 atagtgaaag catcacgttt gttgataatg ataattttaa agctggaaaa gaagcgacgg
    15061 aatatttaat tgaatctggg catgagcgta ttgcttttat tggcggaagt cttgaattag
    15121 tcgttaccat tgaccgttta acaggctatg ataaagcgat tcaaaaagca aacattcctt
    15181 accgggacga gtatgtgatt catgaagaat ttctacagaa gggaggaaaa gaagctgttt
    15241 gcgaactttt atctctaaaa gaacgtccaa cggctctggt agtagcagac gatctaatgg
    15301 cattaggcgt tttaaataca ttggatgaaa tggggatttc agttcctgaa gacatttcga
    15361 ttgtcagttt taataatgtt ctgatcgccg aaatggctag acctgctctc acatctattg
    15421 atattaatat ttttgaactt ggttatcagg ctgtacgctg cttgattaag cagattgaaa
    15481 cgccatatgc tgttgcagaa caagtgcgcg ttcctcacaa aatgattaaa agacagtcct
    15541 gtaaaactat tcaaatgacg aagagataac tttatataca aatagaaaag aggctggaac
    15601 aaaagtattg tagttgaagg aagatccgaa cgagtttgat tcttgatgga gaatcaaact
    15661 tgttcggatc ttttcgttgg gagggtaaac gtaggtagct gcttcttagc tgttgattgg
    15721 gttgatttag gtatgtctca atatattctt tatgccaaag atattggcat ttgaagtgca
    15781 aaaaaaatat acacatgcca attatttttt cctttatcaa aaagaaagaa tgatataagt
    15841 ggtgaaaacc ttgatgacaa ataggctgta aagttttatt aaaagttggc acgctatttg
    15901 cataactata aagtaaacaa agatttgcat actaggaggt atatatcatg gtagtttctt
    15961 attctcatga accatttaca aattttaagg aagaaaacaa taaaaaggca tttcaagaag
    16021 cattaacata tgtaaataca cagcttggca aaacgtaccc acttgtgatc gacggtgaaa
    16081 aagtggaaac tgaagagaaa attacgtctg ttaacccagc gaatataaaa gaagtgattg
    16141 gatatgtctc tacagtaaat aaagatctag cagagcaagc gatgcagtca gctttaaaag
    16201 cttttgaaac gtggaaaaaa tggaaagtag aacatcgagc agatattctt ttccgtgcag
    16261 cggccatcat ccgtcgcaga aagcatgaat tttctagcta tcttgtaaaa gaagcaggaa
    16321 agccttggaa cgaagcagat gctgatacag cagaagcaat tgatttttta gagtactatg
    16381 cacgtcaagt cctgacgtta aaagatggtt ctccagtaca gagtcgagac ggtgagtata
    16441 atcagtacaa ttatattccg cttggcgtag gtattatcat ttcaccattt aacttcccgt
    16501 tagctatcat ggctggaaca gcagtagcag ctatcgtaac aggcaacacg attttattaa
    16561 agccagctga tgccactcct gtagtagcag cgaaatttgt agaagtaatg gaagaagcag
    16621 gtttaccgaa aggcgtatta aactttattc ctggtgctac acctgaaatc ggtgactact
    16681 tagttgaaca tccaaaaaca cgctttgttt catttaccgg ctcgcgcgca gtaggttgcc
    16741 gcatttacga aagagcagca aaagtacagc cgggccaaaa atggctaaaa cgcgttattg
    16801 ctgaaatggg ggggaaagat acggtggttg tagacaaaga tgcggatctt gatttagctg
    16861 cttcttcaat cgtatactct gcatttggtt ttgcagggca aaagtgctcc gctggttctc
    16921 gtgctgttgt gcatcaagat gtgtatgatg aagtgcttga aaaagcagtt gcgctaacaa
    16981 aaacattaac tgttggaacc ccggaagaag tttcaactta tatgggacct gttattcacg
    17041 aggcgtcgta taacaaagta ttatcgtata ttgaaattgg aaaagaagaa ggacggcttg
    17101 ttgcgggagg agaagcagac aattcaactg gatactttat tcagccaact atttttgcgg
    17161 atgtagatga aaaagctcgt ctcatgcaag aagaaatttt tggtccggta gtagcattct
    17221 ctaaagcgcg agattttgat catatgatgg agattgcaaa caatacagaa tatgcattaa
    17281 caggcgcttt attatcaaac aaccgcgagc atattgagcg cgctcgtgaa gagtttcatg
    17341 ttggaaatct gtattttaac agaggatgta cgggagcgat tgttggttac cagccttttg
    17401 gcggatttaa tatgtcagga acggattcaa aagcaggcgg accggattat cttgttcttc
    17461 acatgcaagc aaaaacaaca tctgaaacat tctaattgga aaataaggag gagtaaatat
    17521 gacaactgaa acacaaaaaa cggtgaaaat cattgagcaa acagaaaaat ttggggcaca
    17581 caactatcat ccgctgccga ttgtaattga aaaggcagaa ggcgtatggg tacatgatcc
    17641 agaaaataat aaatatatgg atatgctaag tgcttattcc gctgttaacc aaggacatcg
    17701 tcatccaaaa attattgaag cattaaaacg tcaagctgat aaagtaacgt taacgtcacg
    17761 agcgtttcat aatgatcagc ttggtccatg gtatgaaaag atttgtaaat taacaaataa
    17821 aaatatggct cttccaatga atacgggagc agaagcggtg gaaacagcca ttaaagctgc
    17881 acgccgctgg gcttacgatg tgaaaggcgt tgctgaaaat caagcacaaa tcatcgcatg
    17941 tgtgggcaac ttccacggtc gtacaatgac agctgtttcc ttatcgtctg aagcagaata
    18001 tcaacgtggt tttggaccga tgcttccagg gcttcaaacc attccttatg gtgatttaga
    18061 agcgttaaaa gctgctatta caccaaatac agccgcattt ttaattgagc caattcaagg
    18121 tgaagcaggt atcgttttac ctccagaagg cttcttaaaa gctgcagctg atttatgtaa
    18181 agaacataat gtgctattta ttgctgatga aatccaagtt ggcttagcgc gtacaggcaa
    18241 aatgtttgct tgtgattggg aaggcgtaga accagatatg ctgattttag gtaaagcgct
    18301 tggaggagga gttttcccta tttcttgcgt agtcgctaac gaagacattt taagtgtctt
    18361 taaccctggc tctcatggtt caacatttgg aggaaacccg cttgcttgtg ccgtatcatt
    18421 agctgctttg gaagtaattg aagatgaaga acttccagcg cgttcattag aattaggttc
    18481 ttatttcaag cagaagctcc aaagcttaag caatccagtt attaaagaag tgcgcggccg
    18541 aggtctattc atcggggtag aattacacga agaagctcgt ccttattgtg aaaaattaaa
    18601 agaccaaggt ttgctttgta aagaaacgca cagtacggtt attcgttttg ctccgccgct
    18661 aacgatttca aaagaagagt tagactgggc aattgaacga attgaaaaag tatttgctga
    18721 gtaatcggtt taaaataaat aaaaatagta cgatatgtat atatcgtact atttttattc
    18781 atatgcagct aaatgacgtt tttatttgat aggcaggagg aatagaagtg tatatccaac
    18841 tctcacagct aagcatggaa gagcaagttg aatgtttaac aaaagcgtta gtgaaaatca
    18901 aaagtatcaa tgggacaagc ggcgaagtaa agattgctga tttcattaaa aacacgcttc
    18961 aaagttttcc ttatttcaaa gaaaatcccc ttcacgtttg ggaacagaaa attaatggcg
    19021 atacgctagg tagaaaaaat gtatttgctt ttattcaagg cagcaaaaaa aatgctcaaa
    19081 caatgattta tcacgctcac ttagatacgg ttggcattga agattttggt ccattaaaag
    19141 acatagcatt agacccagaa gaacttcagc gctatttctc tatgcataac attaatgaag
    19201 atgtacagcg agatgctcgc tcaggtgaat ggatgtttgg aagaggagcc gttgatatgc
    19261 aaagtggaat tgctgtacat ttagccaatg tgctgcattt tactaagcat ccaaataaat
    19321 tagaaggaaa cgtccttttt atggtcaacc cagatgagga aagtcagcat gctggaatta
    19381 tttctgctgt ttctgaactg aaccgtttga aaaaagaaaa aaatctttca tatgtagcag
    19441 cgattaatac cgactttatt acacctcttt acgatggaga ttccacgcgc tatatttata
    19501 caggagccgc tgggaaactg cttccatgtt tttatattta cggaagggag gtacacgtag
    19561 gagatacgct tgctggtata gatcctaatt tgattgcatc tgagattacg ggaagcatcc
    19621 ataacaatat taatctagct gaaaatattg aaggagagct ggtactgcca ccttcctgtc
    19681 tgtatcaacg agataataaa gaggcttata atgtgcaaac tgctgtaagc agtcatttgt
    19741 attttaacta tttcatctat gaacgaacgg caaaagaagt gatgagtcaa ttaattggat
    19801 tatcaatcga agcatgtgaa aaagtggaga aaaaactttc tgattattac gaactttttg
    19861 ttcggagaac gaatttacca aagcgaaact tgtcttggaa agtggatgtt gtgagtttgg
    19921 aagattacct ggaagaattg gatcaaaaag ggataaatgc tcaggcttgt attgaacgag
    19981 ctttcgaaca gtatgcgcat ttagaactta gaacaaggtg ctttaaagta attgaagaac
    20041 ttcaaaagtt agatccgcat caaagtccgc gcgtaattgt cttttttgca ccgccttact
    20101 tgcctcataa tcacttgaag aaagacgaaa gcagagatca gcagcttctt tatcccttgc
    20161 aagcagctgt ggagaaggtt ggaaaacaga cgggagagac gtttcagttt aaaaaatttt
    20221 ttccctacct agcggatggc agctttttat ctcttcatga gacagatgag gaaatcgatg
    20281 cttttacacg taactttccg gggtggggga ttgcaggggc gattcctttc cgtgaaattc
    20341 gagagttaaa tattccgtcc atcaatatag gagtatatgg gaaagacgga cataagtgga
    20401 cagagcgagt gtataagcct tattcgttcg gagttcttcc taagttaatt caagaaacaa
    20461 ctgttcagat gctaaaaagt aggcatgctt gtacaaacca catataagaa aatgaagaac
    20521 ggaggtaccg atgaatagac atacagaaca aaaccaatta cagagaacaa tgaaaagcag
    20581 acatttattt atgattgcgt taggaggcgt gattggaaca ggcctatttt taggttcggg
    20641 ttttactatt agccaggcag ggccgggagg agccattctt gcatatataa taggcggctt
    20701 cctcatgtac ttagttatgc tttgtttagg agagctagcg gtagcaatgc cggtttcagg
    20761 ttcctttcaa gaatacgcta caaggttttt aggaccttct acaggcttta tgattggatg
    20821 gctgtactgg ttcagctggg caaatacga
//
