LOCUS       NZ_JAJNPN010000053      6569 bp    DNA     circular CON 22-JUN-2024
DEFINITION  Bacillus amyloliquefaciens strain B3 plasmid unnamed, whole genome
            shotgun sequence.
ACCESSION   NZ_JAJNPN010000053
VERSION     NZ_JAJNPN010000053.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus amyloliquefaciens
  ORGANISM  Bacillus amyloliquefaciens
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus amyloliquefaciens group.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Zhou,W. and Hou,H.
  TITLE     Sequencing and Analysis of the Whole Genome of fermented soybean
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6569)
  AUTHORS   Zhou,W. and Hou,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2021) School of Food Science, Shanghai Ocean
            University, Hucheng Ring Road, Shanghai 201306, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo2 v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023757615.1-RS_2024_06_22
            Annotation Date                   :: 06/22/2024 03:24:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,947
            CDSs (total)                      :: 3,881
            Genes (coding)                    :: 3,770
            CDSs (with protein)               :: 3,770
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 58 of 111
            Pseudo Genes (incomplete)         :: 65 of 111
            Pseudo Genes (internal stop)      :: 17 of 111
            Pseudo Genes (multiple problems)  :: 25 of 111
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:10:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6569
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAJNPN010000053.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6569
                     /collection_date="2019-12-10"
                     /db_xref="taxon:1390"
                     /geo_loc_name="China: Shandong"
                     /isolation_source="fermented soybean"
                     /mol_type="genomic DNA"
                     /organism="Bacillus amyloliquefaciens"
                     /plasmid="unnamed"
                     /strain="B3"
                     /submitter_seqid="B3-plasmid-contig46"
     region          1..6569
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..6569
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..6569
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[1775:4855]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1776..4855
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(271..288)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.53"
                     /tool="antismash"
     gene            complement(310..1779)
                     /locus_tag="LQ568_RS19440"
                     /old_locus_tag="LQ568_19435"
     CDS             complement(310..1779)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029: RND family
                     efflux transporter MFP subunit"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010332544.1"
                     /locus_tag="LQ568_RS19440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ568_19435"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_025650412.1"
                     /transl_table=11
                     /translation="MKRNFFTLQELKDSRELYESKPKPIGLIFVYLLIGILISAIAFSF
                     YGKKEVILKTTADIQYSKPNSNVTNEIAGKVKTVSFKQGDKVKKGDTLFTLDDTEIQTQ
                     RKASNADINDLKEQIALNKTLKKSIENDKNLFSEDDHWSFQDQYLSYKKQINLLETSEE
                     TVKTQKEKIDKHTKNVKNNLNKTKSKVTLKLQRISDLQNSIRRDKSFAKQDDKYSSYYN
                     SYLTSKQNAEQQLKDVEKMKDELDKSSYSEQKENLNGQIESLKQDMLVKLSEEKDELKQ
                     SLDEINSSIESDNTDIDSNDSEQTNYRLSIEQLKTNLLNQTAQKQTDLQTTLKAKEDDL
                     KMIDLQLEKYKIKAANDGYVNVLTNISPGDNLQAGTSILTIIPDKEKLKANIYIPSYEI
                     TNINEKDEVKFHLENTSQTIKGVITNISRDSISNQKGQKFYIAESTIANNEIVKDNGEK
                     IHLKNGMQTNVSIIVDTKRYIDYFLDVLHLH"
     gene            complement(1776..3959)
                     /locus_tag="LQ568_RS19445"
                     /old_locus_tag="LQ568_19440"
     CDS             complement(1776..3959)
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014481436.1"
                     /locus_tag="LQ568_RS19445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ568_19440"
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="WP_025650414.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 4.6e-46, bitscore:
                     144.7, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 1.9e-237, bitscore:
                     778.7, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFFKKYHCVKQMDEKDCGAACLSTIARQYGSKLSITYIRDIAGTD
                     TQGTSVLGMVKAAEQLGFSAKAVRAEHAAFDQSITLPAIAHIVIDQQLLHYVVIHKISK
                     EKIIIADPGKGLIKYKKDDFLKIWTGILILLTPTEAYEKQKEIRNISSDIFRLILQQKS
                     LIIHTFLASFIITIFGILGTFYFQTIIDQILPNGLSNTLHILSIGLLIMYLFKVFLIGF
                     RQYMMILLGQKLSITIMFGYFKHVLSLPMKFFSNRKVGEIISRFTDANKVIDAVVSASL
                     SILLDTIMLIMVGIFLYLQNATLFLITLAFVPLYILTVWIFIKPYANINNTEMENNAQL
                     TSKIVETLNGIETVKAYNAEYMMTFETEKRFVKYLRSAFKHGVIDNLQSSIKMFLDLAS
                     GALILWIGSTQVMKGNLTIGQLITYNALLTYFLNPLQNIIGLQSKFQSASVASKRLGEI
                     IEQDPEIKDNEKRITSISSITGPIVFNKINFRYGTRRLILKNIALQIQKGEKVAFVGES
                     GSGKSTLTKLLMKFYAAESGEILINGYHINDIHTNALRESISYIPQESHFIQGSILENL
                     LLGNSGSFTFEEIIEVCKQTKVHDFIDHLPMRYDTLIEENGSNLSGGQKQRLAIARALL
                     RKPNILIMDESTSNLDTTTEQGISKMIYDQTYNITTIIIAHRLSTIMNCDKIFVMENGE
                     IKESGSHHDLLMKKGKYYELWENQIPSKEMVTI"
     gene            complement(4078..4338)
                     /locus_tag="LQ568_RS19450"
                     /old_locus_tag="LQ568_19445"
     CDS             complement(4078..4338)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LQ568_RS19450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LQ568_19445"
                     /product="hypothetical protein"
                     /protein_id="WP_025650415.1"
                     /transl_table=11
                     /translation="MDFLYMLVETILWIIITTIPSKRNLKFRRNLKSLKKQAWFCELKK
                     NYGPLFLMNSTIQDKIIEYNENNDLETYKEEIENLAKSIHV"
     misc_feature    complement(4381..4399)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 24.12"
                     /tool="antismash"
     gene            4634..4855
                     /locus_tag="LQ568_RS19455"
                     /old_locus_tag="LQ568_19450"
     CDS             4634..4855
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF021923.4"
                     /locus_tag="LQ568_RS19455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ568_19450"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_025650416.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 1.1e-10,
                     bitscore: 30.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKQNNRFESLTVEEAEMIDGGGYGSYLLKGVGTGIAVGAALGGP
                     GGAVLGANAGAIWGSAAYGVDALISGHK"
     gene            4897..5115
                     /locus_tag="LQ568_RS19460"
                     /old_locus_tag="LQ568_19455"
     CDS             4897..5115
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LQ568_RS19460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LQ568_19455"
                     /product="hypothetical protein"
                     /protein_id="WP_025650417.1"
                     /transl_table=11
                     /translation="MLHNFEKSNNMIELSEQEMLDTDGGGWRDLAGAAAGAAVMGGTAM
                     ALGAAGGPVGFAAGIGLAILTRPGHAK"
     gene            complement(5660..5884)
                     /locus_tag="LQ568_RS19465"
                     /old_locus_tag="LQ568_19460"
     CDS             complement(5660..5884)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018664240.1"
                     /locus_tag="LQ568_RS19465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ568_19460"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_025650419.1"
                     /transl_table=11
                     /translation="MNNKVKVERLTLGLTQATLAEKLGITRQTVSLIEKGQYNPSLNLC
                     IKICNVFNKTLDDIFWPSDIKREDRENDR"
     misc_feature    complement(5980..5996)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.37"
                     /tool="antismash"
ORIGIN
        1 tcaaacaagc gctcttggtt atgcttgggg agcgggatat ccgccaaaag taaaagtggt
       61 attgctttga catggcccgg agttaacatc cgggattttt actttttata agatgtgttt
      121 taggaatagg cgggcggaaa aagtgtagca tttcagttgg aggctgtgaa gaattatttt
      181 gcaaacgaga tgaccaagtg ctggatttca aagaagatca taaggtgtag catttggatt
      241 tctgaaaaaa atgcctcaaa gcaaatcttc tttccgcttt gaggcatatg agaactactt
      301 ttaacaaatt tagtgcaagt gcaatacatc taggaaataa tcaatgtatc tttttgtatc
      361 aacgattatt gatacattag tctgcattcc gttttttaag tgtatttttt cgccgttatc
      421 tttaacgatt tcattgtttg ctattgtgct ttctgcaata taaaactttt ggcctttttg
      481 attactaata ctgtcccggc ttatgttagt aattacgcct ttaatggttt gacttgtatt
      541 ttcaagatgg aacttaacct catctttttc attaatattt gtgatctcat aacttggaat
      601 gtaaatgtta gctttaagtt tctctttatc agggataata gtcaaaatag acgttccagc
      661 ttgtaaattg tctcctgggg atatatttgt caatacgtta acatagccat catttgccgc
      721 ttttatttta tatttttcta attgtaaatc tatcattttc agatcatctt cttttgcttt
      781 aagagtggtc tgaaggtctg tttgtttttg ggctgtctga ttcagcaagt ttgttttaag
      841 ctgttcaata gaaagcctat aattcgtttg ctctgaatca ttggaatcaa tgtcagtatt
      901 gtcactttca attgagctat taatctcatc aagggattgt tttaattcgt ctttttcttc
      961 acttagtttt actaacatat cttgttttaa actttcaatt tgtccgttca gattctcttt
     1021 ctgctctgaa taactgcttt tatcaagttc atctttcatc ttttctacat ccttcagctg
     1081 ctgttctgca ttttgcttgc tggtcaaata actattatag taagagctgt atttatcatc
     1141 ctgcttggca aaagacttat ctcttctaat tgagttttgc agatcagaaa ttctttgaag
     1201 ttttaatgta accttgcttt tggttttgtt cagattattt ttcacattct ttgtatgttt
     1261 gtcgattttt tctttctggg tttttacagt ttcttctgat gtctctaaca agtttatttg
     1321 ttttttataa gagaggtatt gatcttgaaa actccaatga tcatcctcgc tgaataagtt
     1381 tttgtcattt tcaatactct ttttaagagt tttgtttaat gcgatttgtt cttttaaatc
     1441 attaatatca gcattagagg cttttctttg tgtttgaatt tcggtatcgt ccagagtaaa
     1501 caaagtgtct ccctttttca ccttatcccc ttgtttaaaa gaaactgttt ttactttacc
     1561 cgctatttca tttgttacat tgctgttagg cttactgtac tgaatatcag ccgttgtttt
     1621 tagaatgact tcttttttgc catagaaact gaaagcaatg gctgatatta atatgcctat
     1681 gagtaaatat acaaagatta gaccgattgg ttttggcttt gattcataaa gttctctgct
     1741 gtctttcaac tcttgtaatg tgaaaaagtt tcgtttcata ttgtcaccat ctcttttgat
     1801 ggtatttggt tttcccacag ttcatagtac ttgccttttt tcattaacaa atcatgatga
     1861 cttccagact ctttgatttc tccattttcc atgacaaata ttttgtcaca attcatgatt
     1921 gtacttaaac gatgagcgat gataatcgtt gttatattat aagtctgatc atatatcatc
     1981 tttgatattc cttgttcagt agtcgtatca agattgcttg tggattcatc cataatgaga
     2041 atgttaggtt ttctcaataa tgctcttgca attgccagtc tttgcttctg accgccagat
     2101 aagttagatc cgttttcttc tataagagta tcgtatctca ttggtaaatg atcaataaaa
     2161 tcgtgtactt tcgtttgttt gcaaacttct ataatttcct caaaggtaaa acttcctgaa
     2221 ttcccgagca aaaggttttc aagaatagat ccttgtataa aatgtgattc ttgaggtata
     2281 tagcttatgc tttcccttaa cgcgttagtg tggatatcat ttatatgata cccattgatt
     2341 aaaatctcgc ctgactcggc agcataaaac ttcattaaaa gcttagtcaa agtagatttc
     2401 cctgaaccgc tttctccgac aaaggcaact ttttcccctt tttgaatttg cagtgcaata
     2461 ttttttagga tgagccttcg agttccgtag cggaaattaa ttttattaaa aacaatgggg
     2521 ccggttattg aagaaattga tgttattcgt ttctcattgt ctttgatttc tggatcttgc
     2581 tctataattt caccgagtct tttagaagcg acagaagctg attgaaactt ggattgaagg
     2641 ccgataatgt tttgtaaagg atttaaaaag taggttaata aggcattata cgtaatgagc
     2701 tgaccgatgg tgagattgcc tttcattact tgggtagacc cgatccacag tatcaatgca
     2761 ccgcttgcta aatctaaaaa catttttatg gaactctgga gattatcaat aactccatgt
     2821 ttaaaagctg accgtaagta tttcacaaac cttttttctg tttcaaaagt catcatatat
     2881 tcagcattgt aagccttaac tgtttcaatg ccgttaagcg tttcaacaat tttcgatgtt
     2941 agctgggcat tgttttccat ttcggtgtta tttatatttg catagggctt tataaagatc
     3001 caaactgtca gaatataaag tggtacgaaa gctaaagtga tgagaaacaa cgtagcattc
     3061 tgcaggtaca gaaatatacc gaccataata agcataatcg tatcgagcag gatagaaagg
     3121 gatgcactga caacagcatc aattacttta ttagcatctg taaatctcga aatgatttct
     3181 ccgacttttc ggttgctgaa aaacttcatt ggtaaagaaa gtacatgttt gaagtatccg
     3241 aacataatgg taatacttaa tttctgaccc agcaggatca tcatatattg cctaaatcct
     3301 attaagaaaa ctttgaatag atacataatt aaaaggccga ttgaaagaat atgtaatgtg
     3361 ttacttagcc cattcggcaa gatttgatca ataatcgttt gaaaataaaa agtacccaga
     3421 attccgaaga ttgtaataat aaaagatgct aagaatgtat gtataatcaa ggatttttgt
     3481 tgaagaatta acctgaaaat atctgaactt atattcctta tctctttttg tttttcatag
     3541 gcttcggtgg gtgttaaaag tattaagatc cctgtccaga tctttaaaaa atcatctttt
     3601 ttgtatttaa taagcccttt gccggggtct gcaatgatta tcttttcttt actgatttta
     3661 tgtataacaa cataatgcaa taattgttga tctataacaa tatgtgctat tgccggaaga
     3721 gtaatagatt gatcgaaagc tgcatgctct gctcgcaccg cctttgcaga gaaccccaat
     3781 tgttcagcgg cttttaccat tccaagaacg ctagttccct gtgtatcagt tccggcaatg
     3841 tcacggatat aagttataga gagctttgaa ccatattgtc ttgcaatggt tgataaacaa
     3901 gcagctccac aatctttttc atccatttgt tttacacagt gatatttttt aaaaaacatt
     3961 tttaatctcc atattataga attgttgaat tatacaaaaa ttattatcat aatacaataa
     4021 gtatgaatat atcaatagtt ccgggaggaa atatatttgt ttttgaataa aaaaatttta
     4081 tacatgtata ctttttgcta aattttctat ctcttcctta taagtttcca aatcattatt
     4141 ttcattatat tcaataatct tgtcttgaat agttgaattc attaaaaaca acggtccgta
     4201 attctttttt aattcacaaa accatgcttg tttttttaga ctttttaagt ttcttctaaa
     4261 tttcaaattt ctctttgatg gtatggtagt aataataatc cataatatag tctcaactag
     4321 catgtacaaa aaatccatca aatgtcctcc ttgagcttaa ttcacaatgt aaataaaaca
     4381 taatgtaaga ctaacataaa agtactttta gttaaagttt caattaatta atcagagtat
     4441 aagattaaat cttcttcagt gagaatcata aaaaagagag tggttattgt aacaaataga
     4501 cgttttgtag gaattttaga tgaaaaatgt catgtaattt tgcttttgag tattgaaaaa
     4561 aagtgtaaat cacatttata ctaatacaag tcgacaataa tttaccaagt tcggacttag
     4621 gaggagtagt agtatggaaa agcaaaacaa tagatttgag tctttaacag tagaagaagc
     4681 tgaaatgata gatggcgggg gatacggttc ttacttatta aaaggagtcg gaacaggaat
     4741 tgctgttgga gcagcgcttg gaggacctgg cggggctgtt ttaggtgcta atgctggagc
     4801 tatctggggt tctgcagcgt atggtgtaga tgcattaatt tctggtcata aataaaaaac
     4861 ataaattgaa caaaaattat atataggagg tttattatgt tacataactt tgaaaaaagt
     4921 aataatatga ttgaattaag tgaacaagaa atgctagata ctgacggcgg gggctggaga
     4981 gatttagcag gcgctgctgc aggcgctgca gtaatgggag gtacagcaat ggctttagga
     5041 gctgctggtg gcccagtagg ttttgctgcg ggaatagggc tggcaatctt aacaagacca
     5101 gggcatgcaa aataatttgt gttatagaaa agctgtctta tgccgacagc ttttctgtat
     5161 taaaaggaat cttaactgca tacctttcca tgcagtttta aattgaagaa cgaaaactaa
     5221 ataatttgtg ttaattcctc acaaggttta cttagccaat ttaatggact aattagattt
     5281 tttatgagaa tacttaatca aagaatggac acttagaaat aatacaatgt agaaaaatga
     5341 gaataataat aactgaatca gtaattctct cacattggta atttctttag aaagaaaact
     5401 tatcattacc caaaataatc cgctcaatat aaaaataaag aaatgtttta atctttttct
     5461 ttttctctgt attaataacc ccctttttat tgcgaaaatt gaataaaaag tgctgacaac
     5521 taaaaacgat ataattatac ttagttgagt gtaaaataag tttttggtta gaatagaaac
     5581 aaaaaaacta agaataaata aaattataga cagtccgaaa ctgtaatagc tcagtttata
     5641 ttgttcgtgt tgtattcttt catctatcat tctctctatc ctctctctta atatcagaag
     5701 gccaaaaaat atcatctaaa gtcttattaa aaacattaca aatttttata cataagttta
     5761 aactggggtt atactgtcct ttttctatta agctcacagt ttgtcttgtt attccaagtt
     5821 tttcagccaa tgttgcttga gttaaaccaa gtgttaatct ttcgacttta actttattat
     5881 tcatggcatc ctcctttatt aattaatatt gtaacatata tattcctttt tgcaaaatat
     5941 atattgcgta gatttggatt ctaaaactga aataagctat tggaacactt ttgcaagtta
     6001 agtgactttc aataaatgac tgtgttttga gaagaatttc ttgtattgta catgagaatg
     6061 gatatttaag gatggtgtct ttgcatatag acgttatcat ctctttaaga ggattgtttc
     6121 caaagtggaa attcattatg acaatgaaca agatagatac atttctcgaa atcagcaaac
     6181 actacatact caacaactga ctacaaggct gccggtcata tcaatcaaat cctgaaatat
     6241 gttccatgtc cactgcttga gaactgcaca aaatcaaaga accggaagaa agttatatta
     6301 cttagcatgt ataggtaaac catagggaaa aggtcagaca aaatcgcttg tctgtttcaa
     6361 gaaaaaaact ttataaaaaa agaaaataaa aaatagagcg aagctttgca gatattccaa
     6421 acagctgcat gggcttcgct attgcaggtt gggtaaaagc ggaatgtgag cgagcaagtt
     6481 ctccttacat ccgcctgccg gacatgaaga agtttcggcg ccatcctcat gataaggctt
     6541 gcggacgtcc cgtctgatcg cctcgatga
//
