LOCUS       NZ_JAJNPN010000045      8995 bp    DNA     linear   CON 22-JUN-2024
DEFINITION  Bacillus amyloliquefaciens strain B3 contig45, whole genome shotgun
            sequence.
ACCESSION   NZ_JAJNPN010000045
VERSION     NZ_JAJNPN010000045.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus amyloliquefaciens
  ORGANISM  Bacillus amyloliquefaciens
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus amyloliquefaciens group.
REFERENCE   1  (bases 1 to 8995)
  AUTHORS   Zhou,W. and Hou,H.
  TITLE     Sequencing and Analysis of the Whole Genome of fermented soybean
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8995)
  AUTHORS   Zhou,W. and Hou,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2021) School of Food Science, Shanghai Ocean
            University, Hucheng Ring Road, Shanghai 201306, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo2 v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023757615.1-RS_2024_06_22
            Annotation Date                   :: 06/22/2024 03:24:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,947
            CDSs (total)                      :: 3,881
            Genes (coding)                    :: 3,770
            CDSs (with protein)               :: 3,770
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 58 of 111
            Pseudo Genes (incomplete)         :: 65 of 111
            Pseudo Genes (internal stop)      :: 17 of 111
            Pseudo Genes (multiple problems)  :: 25 of 111
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:10:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 8995
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAJNPN010000045.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..8995
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:7656](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..7656)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(<1..4461)
                     /gene="srfAB"
                     /locus_tag="LQ568_RS19755"
                     /pseudo=""
     CDS             complement(<1..4461)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (0-272). E-value:
                     7.7e-98. Score: 319.1. Matches aSDomain:
                     nrpspksdomains_LQ568_RS19755_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (437-839). E-value:
                     1.4e-116. Score: 381.4. Matches aSDomain:
                     nrpspksdomains_LQ568_RS19755_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (945-1014). E-value: 3.8e-23. Score:
                     73.5. Matches aSDomain: nrpspksdomains_LQ568_RS19755_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (1029-1317). E-value:
                     1.4e-104. Score: 341.3. Matches aSDomain:
                     nrpspksdomains_LQ568_RS19755_Condensation_LCL.2"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="LQ568_RS19755"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="AMP-binding (E-value: 8.2e-126, bitscore:
                     407.9, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 3e-17, bitscore:
                     51.1, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1e-95, bitscore:
                     308.3, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYILNQLGQASTSYNVPAVLLLEGSVDKNRLEEAMQALINRHETL
                     RTSFDMADGEVVQTIHKNVSFELETAEGREEDAEELTKAFIRPFALNHAPLVRSKLIRL
                     EEDRHLLLIDMHHIITDGSSMGIFIGDLAKLYQGTELELPKIHYKDFSVWQREKANLDQ
                     HEAYWLDTFKGDLPVLDLPLDFPRPAERSFEGERVIFGLDKQVTAQIKKLLADTDTTMY
                     MFLLAAFQVLLSKYSGQEDIIVGSPAAGRQHPDLQDVPGMFVNTVALRSHPAGKKTFKQ
                     FLDEVKTASLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVLFNMQNMEIPALRLGDLE
                     ISSYSMHHHVAKFDLSLEAAERGEEVGLSFDYAKALFADETIRRWSAHFVNLIKACAEN
                     PDIQLSDASLLSAPEREALLSDEKRTEADLPEGTFVSLFERQAQKTPDLTAVAGGTSLT
                     YRELDERSNRFARHLQACGTGSEDIVAIMMDRSADLITAILGVMKAGAAFLPIDPETPE
                     ERIRYSLEDSGTKLLVVNERNMTAAAVYKGKTVVMEDGEWQNESADRLETEPGADQLAY
                     IIYTSGTTGKPKGVQLEHRNLINYVTWFSREAGLTEADKSVLLSSYAFDLGYTAIFPIL
                     QAGGELHIVPKETYTAPDQLGEYIQKNGITYMKLTPSLFHMIVNTARFTSERRFSPLRL
                     VVLGGEKIITSDVRKFHDVYAHTDFINHYGPTETTIGAIAERINMERLDQYEQRPVIGR
                     PIANTGALVLDGAMQLVPPGASGELYITGKGLARGYLHRPQLTAEKFLSNPFSPDSLMY
                     KTGDVVRRLSDGTIEFIGRADDQVKIRGYRIELKEVETVLLSVNGIQEAVVLAVSEGGL
                     PELCAYYKADSGLKGSELRKRLSETLPSHMLPAYFVQVDRIPLTANGKTDKNALPKPGV
                     SQTAQIASALPETELEEKLCRIWKQTLGTDTLGIDDNFFDYGGHSLKGMMLLANIQAEL
                     DKTVPLKALFEQPTVRLLAAYIEKSAVSEGYRMITPADSADAYPLSSAQKRMYVLNQLD
                     RETISYNMPSVLLMEGEVNISRLQEALNQMINRHESLRTSFIDQKGQPMQKIAEQADID
                     LHIFEAADEEKADLIIQAFIKPFDLSAAPLIRAALVRLNEKKHLLLLDMHHIIADGVSR
                     SMLVKELAHLYKGGSLPSPNLHYKDFAVWQNEPEQAERMKDHERYWLSAFSGELPELNL
                     PTDFPRPPVQSFKGQYVRFRAGRETEKAVRELMESSGATLHMVLHAAFHVFLSKITGQR
                     DIIIGSVTAGRTNAEVQEMPGMFVNTLALRNETRKEQTFAGLLERVKQTNLDALAHQDY
                     PFEDLIGKLDLSRDMSRNPLFQVMVTTEDPDKETLELENLRITPYESNQGTAKFDLTLG
                     GFTDQEGLGLQFEYATDLFKKETIEKWSAGFLRILKQAAESPDRKLPEISLISDAEKQA
                     LLDEWKGKTISVPRDKTVHRLFEETA"
     source          1..8995
                     /collection_date="2019-12-10"
                     /db_xref="taxon:1390"
                     /geo_loc_name="China: Shandong"
                     /isolation_source="fermented soybean"
                     /mol_type="genomic DNA"
                     /organism="Bacillus amyloliquefaciens"
                     /strain="B3"
                     /submitter_seqid="contig45"
     region          1..8995
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..8995
                     /SMILES="NC(C(C)C)C(=O)NC(CC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     CDS_motif       complement(358..471)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19755_0014"
                     /evalue="9.30E-19"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="LQ568_RS19755"
                     /protein_end="1368"
                     /protein_start="1330"
                     /score="59.8"
                     /tool="antismash"
                     /translation="KQTNLDALAHQDYPFEDLIGKLDLSRDMSRNPLFQVMV"
     aSDomain        complement(511..1374)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LQ568_RS19755_Condensation_LCL.2
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.40E-104"
                     /label="LQ568_RS19755_Condensation_LCL.2"
                     /locus_tag="LQ568_RS19755"
                     /protein_end="1317"
                     /protein_start="1029"
                     /score="341.3"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIADG"
                     /tool="antismash"
                     /translation="DAYPLSSAQKRMYVLNQLDRETISYNMPSVLLMEGEVNISRLQEA
                     LNQMINRHESLRTSFIDQKGQPMQKIAEQADIDLHIFEAADEEKADLIIQAFIKPFDLS
                     AAPLIRAALVRLNEKKHLLLLDMHHIIADGVSRSMLVKELAHLYKGGSLPSPNLHYKDF
                     AVWQNEPEQAERMKDHERYWLSAFSGELPELNLPTDFPRPPVQSFKGQYVRFRAGRETE
                     KAVRELMESSGATLHMVLHAAFHVFLSKITGQRDIIIGSVTAGRTNAEVQEMPGMFVNT
                     LALRNET"
     CDS_motif       complement(520..609)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19755_0013"
                     /evalue="6.20E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="LQ568_RS19755"
                     /protein_end="1314"
                     /protein_start="1284"
                     /score="44.3"
                     /tool="antismash"
                     /translation="DIIIGSVTAGRTNAEVQEMPGMFVNTLALR"
     CDS_motif       complement(868..900)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19755_0012"
                     /evalue="1.90E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="LQ568_RS19755"
                     /protein_end="1198"
                     /protein_start="1187"
                     /score="14.1"
                     /tool="antismash"
                     /translation="HYKDFAVWQNE"
     CDS_motif       complement(931..996)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19755_0011"
                     /evalue="6.60E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="LQ568_RS19755"
                     /protein_end="1177"
                     /protein_start="1155"
                     /score="28.3"
                     /tool="antismash"
                     /translation="MHHIIADGVSRSMLVKELAHLY"
     CDS_motif       complement(1195..1305)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19755_0010"
                     /evalue="7.60E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="LQ568_RS19755"
                     /protein_end="1089"
                     /protein_start="1052"
                     /score="50.5"
                     /tool="antismash"
                     /translation="SYNMPSVLLMEGEVNISRLQEALNQMINRHESLRTSF"
     aSDomain        complement(1420..1626)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LQ568_RS19755_PCP.1"
                     /evalue="3.80E-23"
                     /label="LQ568_RS19755_PCP.1"
                     /locus_tag="LQ568_RS19755"
                     /protein_end="1014"
                     /protein_start="945"
                     /score="73.5"
                     /tool="antismash"
                     /translation="EEKLCRIWKQTLGTDTLGIDDNFFDYGGHSLKGMMLLANIQAELD
                     KTVPLKALFEQPTVRLLAAYIEKS"
     aSModule        complement(1420..4461)
                     /complete
                     /domains="nrpspksdomains_LQ568_RS19755_Condensation_LCL.1"
                     /domains="nrpspksdomains_LQ568_RS19755_AMP-binding.1"
                     /domains="nrpspksdomains_LQ568_RS19755_PCP.1"
                     /locus_tags="LQ568_RS19755"
                     /monomer_pairings="Asp -> Asp"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(1915..1980)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19755_0009"
                     /evalue="1.30E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="LQ568_RS19755"
                     /protein_end="849"
                     /protein_start="827"
                     /score="36.4"
                     /tool="antismash"
                     /translation="FIGRADDQVKIRGYRIELKEVE"
     aSDomain        complement(1945..3150)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LQ568_RS19755_AMP-binding.1"
                     /evalue="1.40E-116"
                     /label="LQ568_RS19755_AMP-binding.1"
                     /locus_tag="LQ568_RS19755"
                     /protein_end="839"
                     /protein_start="437"
                     /score="381.4"
                     /specificity="substrate consensus: Asp"
                     /tool="antismash"
                     /translation="FERQAQKTPDLTAVAGGTSLTYRELDERSNRFARHLQACGTGSED
                     IVAIMMDRSADLITAILGVMKAGAAFLPIDPETPEERIRYSLEDSGTKLLVVNERNMTA
                     AAVYKGKTVVMEDGEWQNESADRLETEPGADQLAYIIYTSGTTGKPKGVQLEHRNLINY
                     VTWFSREAGLTEADKSVLLSSYAFDLGYTAIFPILQAGGELHIVPKETYTAPDQLGEYI
                     QKNGITYMKLTPSLFHMIVNTARFTSERRFSPLRLVVLGGEKIITSDVRKFHDVYAHTD
                     FINHYGPTETTIGAIAERINMERLDQYEQRPVIGRPIANTGALVLDGAMQLVPPGASGE
                     LYITGKGLARGYLHRPQLTAEKFLSNPFSPDSLMYKTGDVVRRLSDGTIEFIGRADDQV
                     KIR"
     CDS_motif       complement(2062..2151)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19755_0008"
                     /evalue="6.20E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="LQ568_RS19755"
                     /protein_end="800"
                     /protein_start="770"
                     /score="50.5"
                     /tool="antismash"
                     /translation="PPGASGELYITGKGLARGYLHRPQLTAEKF"
     CDS_motif       complement(2680..2739)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19755_0007"
                     /evalue="4.50E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="LQ568_RS19755"
                     /protein_end="594"
                     /protein_start="574"
                     /score="31.4"
                     /tool="antismash"
                     /translation="AYIIYTSGTTGKPKGVQLEH"
     CDS_motif       complement(2926..2967)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19755_0006"
                     /evalue="5.30E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="LQ568_RS19755"
                     /protein_end="512"
                     /protein_start="498"
                     /score="16.4"
                     /tool="antismash"
                     /translation="LGVMKAGAAFLPID"
     CDS_motif       complement(3484..3597)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19755_0005"
                     /evalue="3.20E-15"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="LQ568_RS19755"
                     /protein_end="326"
                     /protein_start="288"
                     /score="48.4"
                     /tool="antismash"
                     /translation="ASLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVLFNM"
     aSDomain        complement(3646..4461)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LQ568_RS19755_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="7.70E-98"
                     /label="LQ568_RS19755_Condensation_LCL.1"
                     /locus_tag="LQ568_RS19755"
                     /protein_end="272"
                     /protein_start="0"
                     /score="319.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIITDG"
                     /tool="antismash"
                     /translation="MYILNQLGQASTSYNVPAVLLLEGSVDKNRLEEAMQALINRHETL
                     RTSFDMADGEVVQTIHKNVSFELETAEGREEDAEELTKAFIRPFALNHAPLVRSKLIRL
                     EEDRHLLLIDMHHIITDGSSMGIFIGDLAKLYQGTELELPKIHYKDFSVWQREKANLDQ
                     HEAYWLDTFKGDLPVLDLPLDFPRPAERSFEGERVIFGLDKQVTAQIKKLLADTDTTMY
                     MFLLAAFQVLLSKYSGQEDIIVGSPAAGRQHPDLQDVPGMFVNTVALRSH"
     CDS_motif       complement(3652..3741)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19755_0004"
                     /evalue="9.80E-15"
                     /label="C5_LCL_267-296"
                     /locus_tag="LQ568_RS19755"
                     /protein_end="270"
                     /protein_start="240"
                     /score="46.9"
                     /tool="antismash"
                     /translation="DIIVGSPAAGRQHPDLQDVPGMFVNTVALR"
     CDS_motif       complement(3988..4023)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19755_0003"
                     /evalue="2.00E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="LQ568_RS19755"
                     /protein_end="158"
                     /protein_start="146"
                     /score="14.0"
                     /tool="antismash"
                     /translation="HYKDFSVWQREK"
     CDS_motif       complement(4054..4119)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19755_0002"
                     /evalue="1.40E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="LQ568_RS19755"
                     /protein_end="136"
                     /protein_start="114"
                     /score="30.5"
                     /tool="antismash"
                     /translation="MHHIITDGSSMGIFIGDLAKLY"
     CDS_motif       complement(4315..4428)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19755_0001"
                     /evalue="3.60E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="LQ568_RS19755"
                     /protein_end="49"
                     /protein_start="11"
                     /score="51.5"
                     /tool="antismash"
                     /translation="TSYNVPAVLLLEGSVDKNRLEEAMQALINRHETLRTSF"
     gene            complement(4383..4547)
                     /gene="comS"
                     /locus_tag="LQ568_RS19325"
                     /old_locus_tag="LQ568_19320"
     CDS             complement(4383..4547)
                     /codon_start=1
                     /gene="comS"
                     /inference="COORDINATES: protein motif:HMM:NF036673.4"
                     /locus_tag="LQ568_RS19325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ568_19320"
                     /product="competence protein ComS"
                     /protein_id="WP_269195014.1"
                     /transl_table=11
                     /translation="MRARRNSSLNRSDKRLIRSIILFPQHSAGCISLISSDRPARATTS
                     LLYFCWKDQ"
     gene            complement(<4567..7656)
                     /gene="srfAB"
                     /locus_tag="LQ568_RS19330"
                     /old_locus_tag="LQ568_19325"
                     /pseudo=""
     CDS             complement(<4567..7656)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (0-29). E-value: 2.5e-15.
                     Score: 47.4. Matches aSDomain:
                     nrpspksdomains_LQ568_RS19330_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (6-306). E-value:
                     6.3e-113. Score: 368.8. Matches aSDomain:
                     nrpspksdomains_LQ568_RS19330_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (470-864). E-value:
                     6.1e-112. Score: 366.1. Matches aSDomain:
                     nrpspksdomains_LQ568_RS19330_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (970-1029). E-value: 4e-20. Score:
                     63.8. Matches aSDomain: nrpspksdomains_LQ568_RS19330_PCP.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="LQ568_RS19330"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="LQ568_19325"
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 6.3e-92, bitscore:
                     295.9, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.7e-120, bitscore:
                     390.4, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.1e-14, bitscore:
                     43.0, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKKSIQKVYALTPMQEGMLYHALLDPHSSSYFTQLELRIHGSFQ
                     LELFEKSVNELIRTYDILRTVFVHQQLQKPRQVVLAERKTKVHYEDISQLDEARQTEYI
                     ERYKRSVQQQGFHLAKDILFKAAVFRLSEKELYLVWSNHHIVMDGWSMGVLMKSLFQNY
                     EALRAGRPAGGSQGKPYSDYIKWLGGRDYEEAEQYWSSRLADFEQPSLLPGRLAAEKKD
                     YQNEEYSFVWDEELVAQIQQTANRHQVTGPNLFQAVWGAVLSKYNYTDDVVFGTVVSGR
                     PSEINGIETMAGLFINTIPVRIKIDKDAAFSDVMAAVQKNAVEAERYDYVPLYDIQKRS
                     ALDGSLLNHLVAFENYPLDKELENGGMEERLGFSIKVEHAFEQTSFDFNLIVYPGKTWT
                     VKIKYNGAAFAHDAIERTAHHLTRMMKAAVGTPDAPVRELGLVSGKEERQIVEVFNDTK
                     TALPEEEAVHRLFEAQANRTPASSAIKEAGREWTYREVNEAANRLARRLVKSGLEKGRT
                     AAIMNDRSAETVIGMLAVLKAGGAYVPIDPAFPEDRLRFMAEDSSIRLVLTVQAYQEQA
                     GALQVPVVMLDESADETVSGTDLNLPAGGNDLAYIMYTSGSTGKPKGVMIEHRNIIRLV
                     KHSNYVPVHEEDRMAQTGAVSFDAGTFEVFGALLNGASLHPVKKETLLDAGQFAQFLKE
                     QRITTMWLTSPLFNQLAQKDAGMFNTLRHLIIGGDALVPHIVSKVRKASPELSLWNGYG
                     PTENTTFSTSFLIDQDYDGSIPIGKPIGNSTAYIMDENRNLQPIGAPGELCVGGSGVAR
                     GYVNLPELTEKQFVRDPFRPDEMIYRTGDLAKWLPDGTIEFLGRIDNQVKVRGFRIELG
                     EIEAKISQAENVTESAAVIRKNKADENEICAYFTADQALSPEDLRKTLSESLPEYMIPA
                     HFIQMNQFPLTANGKIDKKALPEPQAEAVQKEYEAPKTEAEQKLADIWEGILGVKAGVT
                     DNFFTIGGHSLKAMMMTAKIQEHFQKEVPIKVLFEKPTIQE"
     aSDomain        complement(4570..4746)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LQ568_RS19330_PCP.1"
                     /evalue="4.00E-20"
                     /label="LQ568_RS19330_PCP.1"
                     /locus_tag="LQ568_RS19330"
                     /protein_end="1029"
                     /protein_start="970"
                     /score="63.8"
                     /tool="antismash"
                     /translation="EQKLADIWEGILGVKAGVTDNFFTIGGHSLKAMMMTAKIQEHFQK
                     EVPIKVLFEKPTIQ"
     aSModule        complement(4570..7638)
                     /complete
                     /domains="nrpspksdomains_LQ568_RS19330_Condensation_DCL.1"
                     /domains="nrpspksdomains_LQ568_RS19330_AMP-binding.1"
                     /domains="nrpspksdomains_LQ568_RS19330_PCP.1"
                     /locus_tags="LQ568_RS19330"
                     /monomer_pairings="Val -> Val"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(5035..5100)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19330_0009"
                     /evalue="1.50E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="LQ568_RS19330"
                     /protein_end="874"
                     /protein_start="852"
                     /score="39.4"
                     /tool="antismash"
                     /translation="FLGRIDNQVKVRGFRIELGEIE"
     aSDomain        complement(5065..6246)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LQ568_RS19330_AMP-binding.1"
                     /evalue="6.10E-112"
                     /label="LQ568_RS19330_AMP-binding.1"
                     /locus_tag="LQ568_RS19330"
                     /protein_end="864"
                     /protein_start="470"
                     /score="366.1"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FEAQANRTPASSAIKEAGREWTYREVNEAANRLARRLVKSGLEKG
                     RTAAIMNDRSAETVIGMLAVLKAGGAYVPIDPAFPEDRLRFMAEDSSIRLVLTVQAYQE
                     QAGALQVPVVMLDESADETVSGTDLNLPAGGNDLAYIMYTSGSTGKPKGVMIEHRNIIR
                     LVKHSNYVPVHEEDRMAQTGAVSFDAGTFEVFGALLNGASLHPVKKETLLDAGQFAQFL
                     KEQRITTMWLTSPLFNQLAQKDAGMFNTLRHLIIGGDALVPHIVSKVRKASPELSLWNG
                     YGPTENTTFSTSFLIDQDYDGSIPIGKPIGNSTAYIMDENRNLQPIGAPGELCVGGSGV
                     ARGYVNLPELTEKQFVRDPFRPDEMIYRTGDLAKWLPDGTIEFLGRIDNQVKVR"
     CDS_motif       complement(5182..5271)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19330_0008"
                     /evalue="2.30E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="LQ568_RS19330"
                     /protein_end="825"
                     /protein_start="795"
                     /score="48.7"
                     /tool="antismash"
                     /translation="PIGAPGELCVGGSGVARGYVNLPELTEKQF"
     CDS_motif       complement(5773..5832)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19330_0007"
                     /evalue="1.80E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="LQ568_RS19330"
                     /protein_end="628"
                     /protein_start="608"
                     /score="35.6"
                     /tool="antismash"
                     /translation="AYIMYTSGSTGKPKGVMIEH"
     CDS_motif       complement(6019..6060)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19330_0006"
                     /evalue="2.50E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="LQ568_RS19330"
                     /protein_end="546"
                     /protein_start="532"
                     /score="20.4"
                     /tool="antismash"
                     /translation="LAVLKAGGAYVPID"
     CDS_motif       complement(6586..6702)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19330_0005"
                     /evalue="2.30E-17"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="LQ568_RS19330"
                     /protein_end="357"
                     /protein_start="318"
                     /score="55.4"
                     /tool="antismash"
                     /translation="QKNAVEAERYDYVPLYDIQKRSALDGSLLNHLVAFENYP"
     aSDomain        complement(6739..7638)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LQ568_RS19330_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="6.30E-113"
                     /label="LQ568_RS19330_Condensation_DCL.1"
                     /locus_tag="LQ568_RS19330"
                     /protein_end="306"
                     /protein_start="6"
                     /score="368.8"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIVMDG"
                     /tool="antismash"
                     /translation="QKVYALTPMQEGMLYHALLDPHSSSYFTQLELRIHGSFQLELFEK
                     SVNELIRTYDILRTVFVHQQLQKPRQVVLAERKTKVHYEDISQLDEARQTEYIERYKRS
                     VQQQGFHLAKDILFKAAVFRLSEKELYLVWSNHHIVMDGWSMGVLMKSLFQNYEALRAG
                     RPAGGSQGKPYSDYIKWLGGRDYEEAEQYWSSRLADFEQPSLLPGRLAAEKKDYQNEEY
                     SFVWDEELVAQIQQTANRHQVTGPNLFQAVWGAVLSKYNYTDDVVFGTVVSGRPSEING
                     IETMAGLFINTIPVRIKID"
     CDS_motif       complement(6751..6846)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19330_0004"
                     /evalue="7.50E-20"
                     /label="C5_DCL_263-294"
                     /locus_tag="LQ568_RS19330"
                     /protein_end="302"
                     /protein_start="270"
                     /score="63.4"
                     /tool="antismash"
                     /translation="DVVFGTVVSGRPSEINGIETMAGLFINTIPVR"
     CDS_motif       complement(7168..7233)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19330_0003"
                     /evalue="3.60E-12"
                     /label="C3_DCL_135-156"
                     /locus_tag="LQ568_RS19330"
                     /protein_end="163"
                     /protein_start="141"
                     /score="38.6"
                     /tool="antismash"
                     /translation="NHHIVMDGWSMGVLMKSLFQNY"
     CDS_motif       complement(7450..7566)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19330_0002"
                     /evalue="2.40E-14"
                     /label="C2_DCL_024-062"
                     /locus_tag="LQ568_RS19330"
                     /protein_end="69"
                     /protein_start="30"
                     /score="45.6"
                     /tool="antismash"
                     /translation="SYFTQLELRIHGSFQLELFEKSVNELIRTYDILRTVFVH"
     aSDomain        complement(7570..7656)
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LQ568_RS19330_NRPS-COM_Nterm.1"
                     /evalue="2.50E-15"
                     /label="LQ568_RS19330_NRPS-COM_Nterm.1"
                     /locus_tag="LQ568_RS19330"
                     /protein_end="29"
                     /protein_start="0"
                     /score="47.4"
                     /tool="antismash"
                     /translation="MSKKSIQKVYALTPMQEGMLYHALLDPHS"
     CDS_motif       complement(7591..7629)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19330_0001"
                     /evalue="2.40E-05"
                     /label="C1_dual_004-017"
                     /locus_tag="LQ568_RS19330"
                     /protein_end="22"
                     /protein_start="9"
                     /score="16.9"
                     /tool="antismash"
                     /translation="YALTPMQEGMLYH"
     CDS             complement(7678..>8993)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Epimerization (0-275). E-value:
                     4.4e-152. Score: 499.5. Matches aSDomain:
                     nrpspksdomains_LQ568_RS19335_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (280-434). E-value: 7.2e-54.
                     Score: 173.8. Matches aSDomain:
                     nrpspksdomains_LQ568_RS19335_TIGR01720.1"
                     /codon_start=2
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057766256.1"
                     /locus_tag="LQ568_RS19335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LQ568_19330"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_252053162.1"
                     /sec_met_domain="Condensation (E-value: 2.3e-77, bitscore:
                     248.1, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="NQSVMLHAKDGFDPEITEKTLHVLTVHHDALRMIYREQKPYYRGL
                     EDVSVELNVFELNGPAEDHEDRIEREADRLQSSISLETGHLLKAGLFRAEDGDHLLLAI
                     HHLVVDGVSWRILLEDFTSVYTQLKQGNEPALPPKTHSFAEFAERIKEYANTKAFLKEA
                     DYWRELEEKEVCTQLPKDRQSGDQRMKHTRTVSFSLTPEQTEQLTTNVHEAYHTEMNDI
                     LLTALGLALKEWTGEDTIGVHLEGHGREDILDGLNITRTVGWFTSMYPMILEMKHADDL
                     SYQLKQMKEDIRHIPNKGVGYGILRYVTAPEHKENLSFEIDPDISFNYLGQFNEMSDSG
                     LFTRSGMPSGQSLSPDTEKPNALDIVGFIENGQMTMTFAYHSLEFHEKTIQSFSDSFKG
                     HLLKIIDHCLAQDGPELTPSDLGDDDLTLDELDKLMEIL"
     gene            complement(7678..>8995)
                     /locus_tag="LQ568_RS19335"
                     /old_locus_tag="LQ568_19330"
     aSDomain        complement(7690..8151)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LQ568_RS19335_TIGR01720.1"
                     /evalue="7.20E-54"
                     /label="LQ568_RS19335_TIGR01720.1"
                     /locus_tag="LQ568_RS19335"
                     /protein_end="434"
                     /protein_start="280"
                     /score="173.8"
                     /tool="antismash"
                     /translation="LSYQLKQMKEDIRHIPNKGVGYGILRYVTAPEHKENLSFEIDPDI
                     SFNYLGQFNEMSDSGLFTRSGMPSGQSLSPDTEKPNALDIVGFIENGQMTMTFAYHSLE
                     FHEKTIQSFSDSFKGHLLKIIDHCLAQDGPELTPSDLGDDDLTLDELDKL"
     aSModule        complement(7690..8991)
                     /domains="nrpspksdomains_LQ568_RS19335_Epimerization.1"
                     /domains="nrpspksdomains_LQ568_RS19335_TIGR01720.1"
                     /incomplete
                     /locus_tags="LQ568_RS19335"
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       complement(7996..8025)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19335_0004"
                     /evalue="2.90E-03"
                     /label="NRPS-E7"
                     /locus_tag="LQ568_RS19335"
                     /protein_end="332"
                     /protein_start="322"
                     /score="10.1"
                     /tool="antismash"
                     /translation="PDISFNYLGQ"
     CDS_motif       complement(8071..8127)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19335_0003"
                     /evalue="3.00E-09"
                     /label="NRPS-E6"
                     /locus_tag="LQ568_RS19335"
                     /protein_end="307"
                     /protein_start="288"
                     /score="29.1"
                     /tool="antismash"
                     /translation="KEDIRHIPNKGVGYGILRY"
     aSDomain        complement(8167..8991)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_LQ568_RS19335_Epimerization.1"
                     /evalue="4.40E-152"
                     /label="LQ568_RS19335_Epimerization.1"
                     /locus_tag="LQ568_RS19335"
                     /protein_end="275"
                     /protein_start="0"
                     /score="499.5"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="NQSVMLHAKDGFDPEITEKTLHVLTVHHDALRMIYREQKPYYRGL
                     EDVSVELNVFELNGPAEDHEDRIEREADRLQSSISLETGHLLKAGLFRAEDGDHLLLAI
                     HHLVVDGVSWRILLEDFTSVYTQLKQGNEPALPPKTHSFAEFAERIKEYANTKAFLKEA
                     DYWRELEEKEVCTQLPKDRQSGDQRMKHTRTVSFSLTPEQTEQLTTNVHEAYHTEMNDI
                     LLTALGLALKEWTGEDTIGVHLEGHGREDILDGLNITRTVGWFTSMYPMILEM"
     CDS_motif       complement(8173..8220)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19335_0002"
                     /evalue="1.30E-08"
                     /label="NRPS-E5"
                     /locus_tag="LQ568_RS19335"
                     /protein_end="273"
                     /protein_start="257"
                     /score="26.8"
                     /tool="antismash"
                     /translation="ITRTVGWFTSMYPMIL"
     CDS_motif       complement(8629..8694)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_LQ568_RS19335_0001"
                     /evalue="1.40E-12"
                     /label="NRPS-E2"
                     /locus_tag="LQ568_RS19335"
                     /protein_end="121"
                     /protein_start="99"
                     /score="39.7"
                     /tool="antismash"
                     /translation="LLLAIHHLVVDGVSWRILLEDF"
ORIGIN
        1 ggccgtttct tcaaagagac ggtgaaccgt tttgtcccgc ggcacagaaa ttgtttttcc
       61 tttccattcg tcaaggaggg cttgtttttc agcatcgctg atcagtgaaa tctcaggcag
      121 ctttctgtcc ggactttctg ccgcttgctt cagaatccgc aggaacccgg cgctccactt
      181 ttcaattgtt tcttttttaa acagatcagt cgcatattca aactgaaggc cgaggccttc
      241 ttgatccgta aatccgccga gtgtcagatc gaatttcgcc gtgccttggt ttgactcata
      301 cggagtgatg cgcaggttct ccagttccag tgtttcttta tccggatctt ctgtcgtcac
      361 catgacttgg aacaacggat ttcggctcat atctctcgac aggtcaagct ttccgatcag
      421 atcctcaaac ggataatcct gatgcgccag cgcgtcgaga ttggtttgtt tcacccgttc
      481 gagcagcccg gcgaaggtct gctctttgcg cgtttcattg cgaagcgcaa gcgtattgac
      541 aaacatcccc ggcatttcct gaacttcggc gttcgttctt ccggcggtca ccgagccgat
      601 aatgatatcg cgctgccccg tgattttgct caaaaagaca tggaacgcgg cgtgaagcac
      661 catatgaaga gtcgctcctg atgattccat taattcacgc accgcttttt ccgtttcacg
      721 ccccgctctg aaacggacgt attgtccttt aaaactttga accggcggac gggggaaatc
      781 agtcggcaga ttcaattcag gaagctcgcc ggaaaatgcg gagagccaat aacgctcatg
      841 gtctttcatc cgttcggcct gctcaggttc attctgccag acggcgaagt ctttatagtg
      901 caggttcggc gacggcaggc tcccgccttt gtaaaggtga gcgagctctt tgaccagcat
      961 gcttctggac acgccgtcgg caataatatg atgcatatcc agcagcagca ggtgtttctt
     1021 ttcattcagt ctgacaagag cggcccgaat gagcggagcc gcgctcagat caaacggctt
     1081 aatgaatgcc tgaatgataa gatccgcttt ctcctcgtcc gctgcttcaa aaatgtgcag
     1141 gtcaatgtca gcctgttctg cgatcttctg catcggctga cctttttgat cgataaagga
     1201 cgtccgcaaa gattcgtggc gattgatcat ctgattgagc gcttcctgaa gccttgaaat
     1261 attgacttct ccttccatca gaagaacgga cggcatatta tagctgattg tctcccggtc
     1321 aagctggttc aggacgtaca tccgtttttg ggcggatgat aacgggtacg cgtctgcact
     1381 gtctgccggt gtgatcatcc gatatccctc agataccgcc gatttttcga tatatgcggc
     1441 aagcaggcgg acagtcggct gttcgaacag cgcttttaac ggaaccgttt tgtccagttc
     1501 agcctgaata ttggcgagga gcatcatgcc ttttaaagag tgcccgccgt aatcgaagaa
     1561 attatcgtcg atgccgagcg tatccgtacc gagtgtctgt ttccaaatgc ggcacagctt
     1621 ttcctccaat tccgtttccg gcaaggctga agcaatttgc gccgtttggc tgacgcccgg
     1681 tttcgggagg gcgtttttat cggtttttcc gttggctgtg agcggaatgc ggtctacctg
     1741 cacgaagtaa gccggaagca tatgagacgg cagtgtttcg gaaagccgtt tgcgaagctc
     1801 agagcctttc agcccgctgt cggctttata gtacgcgcac agttccggca gcccgccttc
     1861 gctgactgcg agaacgaccg cctcctgaat gccgtttacg cttaacagca cagtttcaac
     1921 ctcttttaat tcaatgcggt agccccggat tttcacttga tcatccgcac ggccgataaa
     1981 ttcaatcgtc ccgtcagaaa gcctgcggac gacatctccc gttttgtaca tcaggctgtc
     2041 cggtgaaaaa ggatttgaga gaaatttctc cgccgtcagc tgcggacggt gaagatatcc
     2101 tctggcaagc cccttcccgg taatataaag ctcgccgctt gcgcccggag gaacgagctg
     2161 cattgctccg tccaatacaa gcgcgccggt atttgcgatc gggcggccga tgacgggacg
     2221 ctgctcatat tgatcaagac gctccatatt gatccgttcc gcaatggcgc cgatcgtcgt
     2281 ttccgtcggc ccgtagtgat tgatgaagtc ggtatgggcg tatacgtcat gaaacttgcg
     2341 gacatcggac gtgatgatct tttctccgcc gagcaccact aaacgaagcg ggctgaaacg
     2401 gcgttctgac gtaaaacggg ctgtgttgac gatcatatgg aacaatgacg gcgtcagttt
     2461 catatacgta atgccgtttt tctgtatata ctcaccaagc tgatcaggcg cggtgtacgt
     2521 ctctttcggc acaatgtgca gctcgccgcc cgcctgaaga atcgggaata tggccgtata
     2581 gccgagatca aatgcgtaag aagacagcag cacggatttg tcagcttcgg tcaaaccggc
     2641 ttcacggctg aaccatgtaa cataattgat tagattgcgg tgctccagct ggacgccttt
     2701 cggtttgccc gttgttcccg aggtataaat gatataggca agctggtcgg cgccgggctc
     2761 cgtttcaagc cggtcggcgc tttcgttctg ccattctccg tcttccatta cgaccgtttt
     2821 tcctttataa acggcggccg cggtcatgtt tctctcatta acgaccagaa gcttcgttcc
     2881 gctgtcttca agagagtagc ggattctctc ttcaggggtc tccggatcaa tcgggagaaa
     2941 tgccgctccg gctttcatga cgccgagaat cgcggtaatc aagtcggccg aacgatccat
     3001 cataatggcc acgatgtcct cactgcccgt tccgcaagcc tgaaggtggc gggcaaaccg
     3061 gttcgagcgt tcatcaagct cacgatatgt cagacttgtg ccgcccgcaa ccgccgtgag
     3121 atcaggcgtt ttttgcgctt gccgttcaaa cagcgaaaca aaagtgccct caggcagatc
     3181 cgcttccgtc cgtttttcat cagaaaggag cgcttcccgc tcaggagcgg acagcaggct
     3241 tgcgtcggac agctgaatat ccggattttc agcgcaggct ttaatcagat tgacaaaatg
     3301 agcgctccag cggcggatgg tttcgtccgc aaataaggct ttcgcgtaat caaagctcaa
     3361 tccgacctct tctccgcgct cagccgcttc taaggataga tcaaatttag cgacatgatg
     3421 atgcatggag taagaagaaa tctccaaatc tccgagccgc agggccggga tctccatgtt
     3481 ctgcatatta aataacacgc tgaacagcgg actccggctt gtgtccctcg ttaacggcag
     3541 tttttcaatt aattcttcaa gcggatagct ttgatgctca aaagcttgaa ggctcgccgt
     3601 tttcacttca tccagaaatt gcttgaacgt ttttttgccg gcaggatgcg accgaagcgc
     3661 cacagtgttg acaaacatgc ccggcacgtc ttggagatca ggatgctgtc ttccggccgc
     3721 cggagacccg actatgatgt cttcctgccc cgaatatttg gacagcagca cttgaaaagc
     3781 ggctaacaag aacatgtaca tcgtcgtatc cgtatcagcc agcagcttct taatctgcgc
     3841 cgtcacctgt ttatcaagcc cgaagatgac gcgttcccct tcaaaactgc gctcggcagg
     3901 acgcgggaaa tcaagcggca gatccagcac cggcagatcg cctttgaacg tatcaagcca
     3961 gtaagcttcg tgctgatcga gatttgcttt ttcacgctgc cagactgaaa aatccttata
     4021 atgaatcttt ggcagctcaa gctccgtgcc ttgataaagc ttcgcaagat caccgatgaa
     4081 aatgcccatt gagcttccgt ccgtaataat gtggtgcatg tcaatcagca gaagatgccg
     4141 atcttcttca agccggatca gcttcgaacg gaccagcggc gcatgattga gcgcaaacgg
     4201 cctgataaag gcttttgtca gctcttctgc atcttcttcc cggccctcgg cggtttcaag
     4261 ctcgaacgac acatttttat gaatggtctg cacgacttct ccatctgcca tgtcaaacga
     4321 cgtacgcagt gtctcatgac ggttgattaa tgcctgcatg gcctcttcaa gacggttttt
     4381 gtctactgat ccttccagca gaagtacagc agggacgttg tagctcgtgc tggcctgtcc
     4441 gagctgatta aggatataca tcctgcgctg tgctgaggaa acaggataat gcttctgata
     4501 aggcgcttgt cggatcggtt caaactgctg ttcctcctcg ctctcatgct ccaaataata
     4561 cgcaagctcc tgaatggtcg gcttttcaaa taacacttta atcggcactt ctttttgaaa
     4621 gtgttcctgg attttagcgg tcatcatcat agcttttaaa gaatgtccgc caatcgtgaa
     4681 gaaattgtca gtcacacccg cttttacacc gagaatgcct tcccaaatgt ccgcgagttt
     4741 ctgctccgct tccgttttcg gcgcttcgta ttctttttga acggcttcag cctgaggctc
     4801 aggcagcgct tttttatcaa tttttccgtt cgccgtcagc ggaaactgat tcatctggat
     4861 gaagtgcgcg ggaatcatgt attccggaag tgattccgaa agcgttttgc gcaggtcttc
     4921 cggcgaaagg gcttggtctg cggtaaagta agcgcagatt tcattttcat ccgctttatt
     4981 tttccggatc acagccgcag attccgtcac attctccgcc tggctgattt tcgcctcgat
     5041 ttcgccgagc tcgattctga aaccgcggac ctttacttga ttgtcaattc tgccgagaaa
     5101 ctcgatcgtg ccgtccggaa gccacttcgc caagtccccc gtccggtata tcatttcatc
     5161 cggtctgaac ggatcgcgga caaactgctt ctccgttagt tcaggcagat tcacatagcc
     5221 tcttgccact ccgcttccgc cgacgcacag ctcaccggga gcgccgatcg gctgaaggtt
     5281 gcggttttcg tccataatgt acgcagtgga atttccgatc ggcttcccga tcgggatcga
     5341 gccgtcgtag tcctgatcaa tgagaaaact cgtcgaaaac gtcgtattct ccgtcggccc
     5401 gtagccgttc caaagcgaca gctccggtga tgccttcctc actttgctga cgatatgcgg
     5461 cacaagcgca tcaccgccga tgatgaggtg ccggagcgtg ttaaacatgc cggcatcctt
     5521 ttgggcaagc tgattaaaca gcggagacgt cagccacatg gtcgtgatcc gctgctcttt
     5581 cagaaattgg gcgaattgtc cggcgtcaag cagcgtctct tttttcaccg ggtgcaatga
     5641 cgctccgttc agcaatgcgc cgaagacttc gaaggttccg gcatcaaagc tgacggctcc
     5701 cgtttgcgcc atccggtctt cttcatgaac cggcacgtaa ttcgaatgtt tgacgagcct
     5761 gatgatattt ctgtgttcaa tcatgacgcc tttcggtttg ccggtcgatc cggatgtata
     5821 catgatatac gccaagtcgt tgccgccggc cggaagattc aagtctgttc cgcttaccgt
     5881 ttcatccgcg ctttcatcca gcatgacgac cgggacttgc aatgcgcccg cttgttcttg
     5941 atatgcctga accgtcagca cgagccgaat cgagctgtct tccgccataa agcggagacg
     6001 gtcctccgga aaagccggat caatcggcac atatgcgccg cctgctttta ggacggcaag
     6061 cattcctata acggtttccg cagaccggtc gttcataata gcggcggtcc ggcctttttc
     6121 cagcccgctc ttcacaaggc gtctcgccag gcggttggcc gcttcgttca cttcacggta
     6181 ggtccattcg cgtcccgctt cttttattgc gctgcttgca ggcgtccggt ttgcctgtgc
     6241 ttcaaacaaa cggtgaacgg cctcctcttc cggaagcgcc gttttcgtat cattgaatac
     6301 ctcaacaatc tgccgctctt ctttgcctga aacaaggccg agttcccgca caggcgcgtc
     6361 aggcgttccg accgcggctt tcatcatgcg ggtcagatgg tgcgccgttc gttcgatggc
     6421 atcatgagcg aaagccgctc cattgtattt gattttgaca gtccacgttt ttcccggata
     6481 cacgatgaga ttgaaatcaa agctcgtctg ctcaaaagca tgttcaactt taatggaaaa
     6541 gcccagtctt tcctccatgc ccccgttttc cagctctttg tccagcggat agttttcaaa
     6601 cgcgaccaaa tgattcaaga ggctgccgtc cagggcggag cgcttttgaa tgtcatagag
     6661 cggcacataa tcatagcgct cggcttccac ggcgtttttc tgaactgccg ccatcacgtc
     6721 cgaaaaggct gcatctttgt caatcttgat tctgacggga atcgtattga tgaacagccc
     6781 cgccatcgtt tcaatgccgt tgatttctga aggacggcct gatacgacgg tgccgaacac
     6841 cacatcgtct gtatagttgt atttgctgag caccgcaccc caaacggcct gaaacaggtt
     6901 aggccccgtg acttgatgcc ggttggcggt ctgctgaatc tgcgcgacca gctcctcatc
     6961 ccagacaaaa gaatattctt cattttggta gtctttcttt tctgctgcca gacggcccgg
     7021 cagcaggctc ggctgttcga aatccgccag gcggctgctc caatattgtt ccgcctcctc
     7081 ataatcccgg cctccgagcc atttgatata gtcagagtaa ggtttgccct ggcttccccc
     7141 ggcgggccgg ccggcacgaa gcgcttcata gttttgaaac aggcttttca tcaaaacgcc
     7201 catactccag ccgtccatga cgatatgatg attgctccag acgagataca gctccttctc
     7261 actgagtctg aacaccgccg ctttaaagag aatatccttc gccagatgaa agccctgctg
     7321 ctgcacgctg cgtttatagc gttcaatata ttcggtttgg cgcgcttcat ctaattgact
     7381 gatatcttca tagtgcacct tcgtcttccg ctccgccaat acgacttggc gcggtttttg
     7441 aagctgctgg tgcacgaata ccgtacgcag gatgtcatat gtccggatca gctcattgac
     7501 gcttttttca aaaagctcca gctgaaagct tccgtgaatc ctaagctcta attgtgtgaa
     7561 gtaggaggaa gaatgcggat caagcagcgc atgatacagc attccctcct gcatcggtgt
     7621 gagtgcatac accttttgaa ttgatttttt gctcatatgt cacctctgtc ttttctgtta
     7681 gagaatttcc attaatttat caagttcatc aagcgtcaga tcatcatcgc caagatcgct
     7741 aggcgtaagc tcaggtccgt cttgggccag gcagtgatct atgattttca agaggtgccc
     7801 tttaaagctg tcactgaacg attgaatggt tttttcatga aattcgagag aatgataggc
     7861 aaacgtcatc gtcatctgcc cgttttctat aaatccgaca atatcaagcg cattcggctt
     7921 ctctgtgtca gggctcagcg attgtcctga cggcatcccg gatcttgtaa ataagccgga
     7981 atccgacatc tcattaaact ggcctaagta gttaaagctg atatccggat caatctcaaa
     8041 tgaaagattc tctttatgct caggcgccgt tacataacgc agaataccgt agccgactcc
     8101 tttgttcggg atgtgtctga tgtcttcttt catttgtttc agctgatatg aaagatcgtc
     8161 ggcgtgcttc atctcaagga tcatcggata catgctcgta aaccatccga ccgtccgggt
     8221 gatattcaag ccgtcaagaa tgtcttcgcg cccgtggcct tccaaatgaa cgccgatcgt
     8281 gtcttcaccc gtccactctt tcagcgccag tccgagcgcc gtgagcagga tgtcgttcat
     8341 ttcggtgtga taggcttcat gtacgtttgt cgtcagctgt tcagtttgtt caggcgtcag
     8401 agagaagctg accgttctcg tatgtttcat gcgctgatca ccagactgcc tgtctttcgg
     8461 aagctgagtg cacacctctt tttcctcaag ctctctccag taatccgctt ctttcagaaa
     8521 cgccttcgta tttgcgtatt ctttgattct ctcggcaaat tcggcgaatg aatgtgtttt
     8581 cggcggcagc gccggttcat tgccttgctt cagctgcgta taaacggacg tgaagtcctc
     8641 cagtaaaatc cgccaagata caccatccac gactaagtga tggattgcga gaagaaggtg
     8701 gtctccatct tcagcccgga agagcccggc cttcagcaga tgtcctgttt ccagtgaaat
     8761 actgctttga agacggtctg cttccctttc gatacggtcc tcatggtctt cagcaggtcc
     8821 gttcagctca aagacgttca gttcaacaga cacatcctca agtcctctgt aatacggttt
     8881 ttgttcacga taaatcatcc ggagcgcatc atggtgcacc gtcaggacat gtaatgtttt
     8941 ttctgttatc tccggatcaa agccgtcttt cgcgtgaagc atgacggatt gattc
//
