LOCUS       NZ_JAGSCE010000012     10285 bp    DNA     linear   CON 25-JUN-2024
DEFINITION  Staphylococcus aureus strain SA314
            NODE_12_length_83844_cov_162.038965, whole genome shotgun sequence.
ACCESSION   NZ_JAGSCE010000012
VERSION     NZ_JAGSCE010000012.1
KEYWORDS    WGS; RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 83844)
  AUTHORS   Tang,J., Zhou,R. and Zou,G.
  TITLE     Community associated MRSA ST59 in yaks: an investigation in Ganzi
            Tibetan Autonomous Prefecture, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 83844)
  AUTHORS   Tang,J., Zhou,R. and Zou,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2021) College of Veterinary Medicine, Huazhong
            Agricultural University, Shizishan Street No. 1, Wuhan, Hubei
            430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023657025.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:44:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,663
            CDSs (total)                      :: 2,606
            Genes (coding)                    :: 2,527
            CDSs (with protein)               :: 2,527
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 42 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 17 of 79
            Pseudo Genes (multiple problems)  :: 15 of 79
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:08:42
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 53036
            Orig. end                         :: 63321
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAGSCE010000012.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10285
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10285
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10285
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5285](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5285
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(210..223)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 21.93"
                     /tool="antismash"
     misc_feature    complement(314..328)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.71"
                     /tool="antismash"
     misc_feature    complement(552..564)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     misc_feature    complement(597..612)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.81"
                     /tool="antismash"
     tRNA            complement(624..712)
                     /anticodon=(pos:complement(53711..53713),aa:Ser,seq:gct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="KC252_RS08125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="KC252_08085"
                     /product="tRNA-Ser"
     gene            complement(624..712)
                     /locus_tag="KC252_RS08125"
                     /old_locus_tag="KC252_08085"
     misc_feature    complement(650..659)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 15.28"
                     /tool="antismash"
     tRNA            complement(715..789)
                     /anticodon=(pos:complement(53791..53793),aa:Asn,seq:gtt)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="KC252_RS08130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="KC252_08090"
                     /product="tRNA-Asn"
     gene            complement(715..789)
                     /locus_tag="KC252_RS08130"
                     /old_locus_tag="KC252_08090"
     gene            complement(931..1500)
                     /locus_tag="KC252_RS08135"
                     /old_locus_tag="KC252_08095"
     CDS             complement(931..1500)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /locus_tag="KC252_RS08135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KC252_08095"
                     /product="competence protein ComK"
                     /protein_id="WP_000287265.1"
                     /transl_table=11
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFKV
                     QKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIWLN
                     MHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMISKNP
                     DQPIDYNKATLNVFEALTRYSLFEDK"
     misc_feature    1567..1580
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.83"
                     /tool="antismash"
     gene            1710..1928
                     /locus_tag="KC252_RS08140"
                     /old_locus_tag="KC252_08100"
     CDS             1710..1928
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /locus_tag="KC252_RS08140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KC252_08100"
                     /product="IDEAL domain-containing protein"
                     /protein_id="WP_000876826.1"
                     /transl_table=11
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELID
                     DALVNKDEAAFNQYTAEYKNLEAFLGE"
     gene            complement(2009..2995)
                     /locus_tag="KC252_RS08145"
                     /old_locus_tag="KC252_08105"
     CDS             complement(2009..2995)
                     /EC_number="6.3.1.20"
                     /GO_function="GO:0017118 - lipoyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /locus_tag="KC252_RS08145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KC252_08105"
                     /product="lipoate--protein ligase"
                     /protein_id="WP_250790403.1"
                     /transl_table=11
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIVG
                     KNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKFTE
                     PIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQNAL
                     KVNPAKIKSKGIKSVRKRVANIQEFLIDPLEIEEFKKIILKTIFGEAEVEEYKLTDEDW
                     ENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIFGDFFG
                     VGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            3194..3370
                     /locus_tag="KC252_RS08150"
                     /old_locus_tag="KC252_08110"
     CDS             3194..3370
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /locus_tag="KC252_RS08150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KC252_08110"
                     /product="YkvS family protein"
                     /protein_id="WP_000214898.1"
                     /transl_table=11
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLPE
                     KTVINHKRYKIVE"
     gene            3385..3987
                     /locus_tag="KC252_RS08155"
                     /old_locus_tag="KC252_08115"
     CDS             3385..3987
                     /EC_number="3.4.26.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /locus_tag="KC252_RS08155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KC252_08115"
                     /product="CPBP family glutamic-type intramembrane protease"
                     /protein_id="WP_001033867.1"
                     /sec_met_domain="Abi (E-value: 2.8e-09, bitscore: 29.7,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAGI
                     SYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVNWK
                     WQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWVAILITAICSSSLFIYLDNWWIVTF
                     IFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     misc_feature    complement(4068..4086)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 25.94"
                     /tool="antismash"
     gene            4301..4363
                     /locus_tag="KC252_RS08160"
     CDS             4301..4363
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /locus_tag="KC252_RS08160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_228764722.1"
                     /transl_table=11
                     /translation="MFIVTFIGIVVAIINLSNKK"
     misc_feature    4933..4947
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 26.38"
                     /tool="antismash"
     gene            5001..5285
                     /locus_tag="KC252_RS08165"
                     /old_locus_tag="KC252_08125"
     CDS             5001..5285
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790905.1"
                     /locus_tag="KC252_RS08165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KC252_08125"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000790905.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 1.3e-25,
                     bitscore: 82.2, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNKIFGAILYLSLALGLSTAAYASTEYAEGGTWSHGVGSKYVWS
                     YYYHGHKGHGATAIGKYRSFSGYTRAGVKAKASATKNNWWVNRAYYNIY"
     gene            5329..7293
                     /locus_tag="KC252_RS08170"
                     /old_locus_tag="KC252_08130"
     CDS             5329..7293
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /locus_tag="KC252_RS08170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KC252_08130"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_250790404.1"
                     /transl_table=11
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIAVTDWN
                     KKYSKNEIYKRINQFAKKENVAIYKSTSNYTNKNVDKDIYVFNKSKATTITPFNAKYNI
                     HYLSDDELLKKDIKGSYFVKDKNFDVSKFINFLKEYGVTAESYKIDHMMIAVGVIKQMN
                     IVVPLSSLLIVYFIYYIFEKNINFKAYAIKYLNGFTLRKVIFENFSKKCTYWVTLIITQ
                     ILLTTSVLWILNYTGNLDLFILRLVLLSCLFILTVSVINLWTFLMLLNLNIANMIKGKQ
                     HFKTIRFINTVCKSILLVLIASVMIENTSVIKDLNKIKETEKYWNVLDDYYTIEFAPYH
                     ETKQSLIDNMLRSEQLVKASEAENNAILFKPKGDSVDNDNFSPDEGNVILVNNQFWSIY
                     HKQFQPDIPIKNQKNNVEVIIPQKFHAMRNEINQAYHSWFEFVQNKNNKENKLSLQFIN
                     KNDYRIFTFDARDSRHLSFIEAPIIVNVQASDLSNDFYYAMISQGGYLFKNYDALVKNI
                     EKYHLDGEISGITNYKDSVMEMYHENNLKLTVLNFSQIIIAIILIIIILFDVKYYFEQH
                     RKLLVIKKLYGYSTLRANYQYLLINNIVVVFIGILTNVILHSHYIMMIFATILVVQILL
                     QICSLYYHGRRFNEVIKEF"
     gene            7296..7616
                     /locus_tag="KC252_RS08175"
                     /old_locus_tag="KC252_08135"
     CDS             7296..7616
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /locus_tag="KC252_RS08175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KC252_08135"
                     /product="YxeA family protein"
                     /protein_id="WP_000668628.1"
                     /transl_table=11
                     /translation="MKFIIAILLGLIVSITIAFTIIHHPILDRFNPFLKTEYSYAKVPK
                     GTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDTPKEVR
                     QE"
     gene            7613..8254
                     /locus_tag="KC252_RS08180"
                     /old_locus_tag="KC252_08140"
     CDS             7613..8254
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /locus_tag="KC252_RS08180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KC252_08140"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000571184.1"
                     /transl_table=11
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLLN
                     TIAGLEKTGKQYVYFNGQLEQFKSNFYRDKLGYLFQNYGLIDNLTVNENLDIGLAYKKI
                     SKKEKEQLKIRYIEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPTGAL
                     DPKTGQMIIQSLFDLVDENKVLILATHDMAIANQCDEIIDLEQYRKVASM"
     gene            complement(8342..8632)
                     /locus_tag="KC252_RS08185"
                     /old_locus_tag="KC252_08145"
     CDS             complement(8342..8632)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /locus_tag="KC252_RS08185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KC252_08145"
                     /product="hypothetical protein"
                     /protein_id="WP_115211034.1"
                     /transl_table=11
                     /translation="MFYACHFKMKIGGVILTRTYNIIGILSCLISFIIMALPMIWYAAS
                     ALWFFPGAIMILLLSLVIVFCYIKTKNQLHLLLIVLNIIILLFFSLPLLLS"
     misc_feature    8746..8759
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 21.07"
                     /tool="antismash"
     gene            8965..9252
                     /locus_tag="KC252_RS08190"
                     /old_locus_tag="KC252_08150"
     CDS             8965..9252
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410715.1"
                     /locus_tag="KC252_RS08190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KC252_08150"
                     /product="hypothetical protein"
                     /protein_id="WP_000410749.1"
                     /transl_table=11
                     /translation="MEKGIVNYDDANILKLDMNQVRCNKLVVDDIFKDYEQIKPTIEIE
                     KGNAILKLNGYFVASILETLNLNRVKKLYVDEDYYYTYNELIVKYTEVKE"
     misc_feature    complement(10167..10181)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.83"
                     /tool="antismash"
ORIGIN
        1 gaaggggttg agtggacaga ttcgaaatga agatttgtat catgcatacc catatccaga
       61 taagccaatg gatatgacaa ttagtggtca aaatatcaaa gatatattgg agtatagtta
      121 ttcacattta gattttgtta acgagcaatt aagcttaaca attattgatg aaacattatg
      181 tacaatgtgg caaggattca attatgaaat tgatatgaat caagaacctg ggcaacgagt
      241 aatgttagat caaattgatt tgactaagag ttatagagtt acaatgactg actattgtta
      301 tcgtaactac aagaattatt tgaaaaatgc tattatacat gaatcatacg atgaaacaat
      361 gagtacatta attgcagaga agttaagaga tccgaattat cgtatttcat gtagtgataa
      421 ttttgtagtt aaaaacaggt aaatattact atgtaataaa ttaattataa gtttaacaag
      481 tataaaaacc tttcttagag aagattatct aggaaaggtt tttagaattt taaaattgat
      541 ataaaagaac agtgaaaatg tcatttatta tatatataag taaacaaaaa agcttaaatg
      601 gtatatacca tttaagcgag taacggaaac ggagggattc gaaccctcgc gccgctttcg
      661 cgacctacac ccttagcagg ggcgcctctt cagccaactt gagtacgttt ccaatggctc
      721 cacaggtagg actcgaacct acgaccgatc ggttaacagc cgatagctct accactgagc
      781 tactgtggat taatttgtaa gtgtttgaat atcaagcaca aatattatta taacaagatt
      841 aagttaattt gcaatagata ccttaaaaaa atatgcagaa catttacact atatttataa
      901 aacattccgc atattttaaa aataaacaat ttatttatct tcaaataaag aatagcgtgt
      961 caatgcttca aacacattca atgtggcttt attataatct attggttggt cgggattttt
     1021 tgatatcatg cgagattgtt tatctaccat gtaatagtaa aaaatggaat tgttatattg
     1081 atgccataaa ctgtggtatg aaacatgaag aatgattgat tcattattaa taaatgtcac
     1141 tttacattta cgatttttta attctttaat actttcgata taatgcatat ttaaccaaat
     1201 attttcattt tgtctgtcag aatgtgttgg gaaaaaataa gttggaaata atggtgttag
     1261 taaaataggt ggtttactag aaatgccagt aatgcggttt gtctctgctt tcttgccaag
     1321 ataagtatta ccataaaatt tgcaagagcg ttcaatgatt ttctggactt taaaaggatt
     1381 ttgaatacgc gttttgtcaa accgaattat ttcgctacca tttctttgat cgtcatcatc
     1441 aaatgctggt cgaataacca tgtctccttt gcgtatcaca taaatatttt gagaatacat
     1501 aagcaaaacc tcgctttatt aagttttaaa ccattaatat aaaaatacaa ttaatgatat
     1561 aaagatattt tataataaaa ttctgagttg tcaagttatt attttggtga aaatgtacaa
     1621 tattttttaa ctaatagtca gaatattcat tgatttcaaa attagaaaaa tgtatactta
     1681 ttataaatta aagaaaggta gtggtttcta tgaaatacaa tactaatgtt aaacatacaa
     1741 ctttagaagc gtttgtcaca accgtcaatg atttgggtat tgaattaatt atcaatgaag
     1801 cacttcgaga ggtaagaaaa cgacagctca tagaacttat agatgacgca ctcgtcaata
     1861 aagatgaagc agcatttaat caatatacgg cagaatacaa aaatttggag gcatttctcg
     1921 gtgaataaca ttgattcgaa ttccataacg caatcataaa aggactattc ttaacatctc
     1981 atacatgtaa gaatagtcct ttaatcgttt atgacattaa tctaattaat tcatgtctat
     2041 ctatgtcacc gaaatagtga tataaatcat attctgatag tgcttcttct atgtgctcaa
     2101 agtcgtgtag acagccaact aatgcatttt caagatcagt gacatctccg acaccaaaga
     2161 aatcaccgaa aatttttgca tgttcgattt tacctcgttt aacatcaaac ttaatttgta
     2221 caaatccttt ttcaaatttt tcctcgcgtt caaagttata tttagggttt ctaccataat
     2281 tccattccca agttctatat ttgtcgttac ttaacttttc aatattttcc caatcttcat
     2341 ctgttaattt atattcttct acttcagctt ctccaaagat agttttcaag atgatttttt
     2401 taaattcttc aatttctaaa gggtcaatta aaaattcttg aatgtttgct actcgtttac
     2461 gaacagattt aatacctttt gatttaatct tagctggatt aacttttagt gcattctgaa
     2521 cttcgtctaa atcactattt aacatcaacg taccatgact aaacattcta tttttaactt
     2581 taaccatggc atttcccgag attttcgctt gtccaacttg aatatcgtta cgaccagtta
     2641 attcagcatt tacgcctaaa gattgtaatg cttgaacaat tggttcagtg aatttttgga
     2701 aattgtggaa actgttacca tcatcatctg ttataaaact aaagtttaaa ttgccagtat
     2761 catgataaac agcgccacca ccagaaattc ttcttactac atcgatgtta tgagcatcta
     2821 tatatgtctg atttacttcc tctatcgtat tttgattctt tccaacaatg atagatggtc
     2881 tatttatgta aaataaaaag taactttctt ctgctggtaa attttttaaa acatattctt
     2941 ccattgctaa gtttaaagtt ggatctgtaa tattattatt actaatgaat ttcattacaa
     3001 tctctccctt atatctatat atattctcta cttatttatg ccttaacttt gctcaaaatg
     3061 ccaattatat actcacaaat cattctaaat atcggcttaa agttacattt tattattttt
     3121 gattgttaat agtttatttg ttaagtgaat tttagtacaa tataatttag ttatgattta
     3181 aggaggactt aaaatgactg ttgcagaagt gggtaacatt gttgagttta tggatggatt
     3241 aagaggtcgt gttgaaaaaa tcaacgataa ctctgttatt gttgacttaa caattatgga
     3301 aaattttaat gaccttgatt taccggaaaa aactgttatc aatcataaac gatataagat
     3361 tgttgaataa gaaggtaagt tataatgaat aaaatctcga aggctttaac ttggtttatt
     3421 ataagtttca ttatatttca tctcatatta tttattatgt ggggcgaaca ccaagaatac
     3481 tggtatttat atacaggtat aatgctaatt gctggtatca gttatgtatt ttatcaaaga
     3541 gatattgaat ctaagaggtt gcttacatca attggtgttg gtattattac ggcaattatt
     3601 ttaattatgc ttcaactttt attctcactt ataacatcta atttaagtta tagttcatta
     3661 attaaagaat tagcaagaac aggtgtcaat tggaagtggc aaatgttagt aactttactt
     3721 tttgtcattc catgtcacga gttatatatg agaactgttt tacaaaaaga attaacacat
     3781 ttttcaatac cgaaatgggt agctatatta ataactgcaa tttgttctag ttcattattt
     3841 atttatttag ataattggtg gatagtaaca tttatatttg ttgcacaagt gatattatca
     3901 ttaagttatg agtatactcg acgtatagca acaacatccg ttgcacaaat tgtagctatt
     3961 atattgttat taatttttaa tgcttaaata agcttttctt agacgtatgt gttctaagaa
     4021 aagctttttg ctttagaaaa gcactacgaa gattcaaggc ttgctttatt atagtgtata
     4081 catttttaac ggcaagcagt tattattgag gttatatttt agtcagtttt ttattactaa
     4141 atattttata aaaatggtat aatattttgt gggcaagttg aattgatggt ggctatctga
     4201 gtaaaggggg ggtgcctatg gcattactta acacttttga aaggaaaagc ctattgtgat
     4261 atctattgca aacgcattac atttaatgtt aagtttcggt atgtttatcg tcactttcat
     4321 tggtatagta gtcgcaataa taaatttaag caataaaaaa taaccatcat ttcaaaactt
     4381 tgacatagaa tgatggttaa agaaaatctc atttagtcac cgtcttttta acgggctcat
     4441 taggcgacat gttcctccat gtcgtctttt tatattttta tagtaacata tcttttattt
     4501 aattgtctat ttttattaga tgtgtaacaa aattttgaag tattttcctt atattgcctg
     4561 ctaaacatgt cagatttgaa aggaaaatca tactatggta gaaatcactt atgattctat
     4621 ggcagtactt atacataagt gatttctatt gaatatgtaa tgaagttcat atattaatat
     4681 aactttcatt ggtatagtat tcgcaatgat taatttaaac aataaaaaat aaccatcatt
     4741 tcaaaacttt gacaaagaat aatggttaaa gcaaatctca tttagtcacc gtctttttaa
     4801 cggattcaat aggcgacatg ttcctgcata tcgtctattt atgaactgca caatttaata
     4861 ataatacaat tatactttga tgtccattta acatgaagta gtttaaattt gtagcaaata
     4921 aattcatctt gaaatattca gaaaattctg tataatttga ataaagagat atagattaaa
     4981 tataaagagg tgttttttaa atgaagaaca aaatttttgg cgctatatta tatttatctt
     5041 tagcacttgg attatcaaca gcagcttatg catctacaga atacgcagaa ggaggcactt
     5101 ggagtcacgg tgtcggcagt aagtatgttt ggtcttatta ttatcatggt cataaaggac
     5161 atggtgcaac agctattgga aaatacagat catttagtgg ttatacaaga gctggtgtaa
     5221 aagcaaaagc atcagctact aaaaataatt ggtgggtcaa tagagcatat tataacattt
     5281 attaaatact aaataatcat agaaaataaa caacgcaaga ggtacaacat gaaatggttc
     5341 aaattaatac ttgatgttac aatcttcatt ctaattgcta tattgttatt tgtttataca
     5401 tataaagaaa acgaagaaat attgcctgat actaaatacc ctatagcggt aactgactgg
     5461 aataaaaaat atagtaaaaa tgagatttat aaacgtataa atcagttcgc taaaaaagag
     5521 aacgtagcaa tctataaatc aacttcaaat tatacaaaca aaaacgtaga taaagatata
     5581 tatgtattta ataaatcaaa agcaacaact atcactcctt ttaatgctaa atataacatt
     5641 cattatttaa gtgacgacga attattaaaa aaagatatca aaggaagtta ttttgtaaaa
     5701 gacaaaaatt ttgacgtgtc taaattcata aattttttaa aagaatatgg tgttactgct
     5761 gagtcataca aaatagatca tatgatgatt gccgttggtg tcattaaaca aatgaatata
     5821 gtagttccgt tatcctcact tttaatcgtt tattttattt attatatttt tgaaaagaac
     5881 attaatttca aagcgtatgc gattaagtat ttaaatggtt ttacattaag aaaagtaatt
     5941 tttgaaaatt tttcaaagaa atgcacgtat tgggtaacgt taatcataac tcaaattctt
     6001 ttaactacaa gtgtactttg gatattaaat tacacgggta atttagattt attcatatta
     6061 agattagttc tgctttcatg tctttttatt ttaacggtta gtgttattaa tttatggact
     6121 ttcttaatgt tactaaattt aaatattgcc aatatgatta aaggtaagca gcactttaaa
     6181 acaattcgtt ttattaatac agtttgtaaa agtattctct tagtactaat agctagtgta
     6241 atgatagaaa atactagtgt tattaaagat ttgaataaaa taaaggaaac tgagaagtat
     6301 tggaatgtat tagatgatta ttacacgatt gaatttgcac cttatcatga aacgaaacaa
     6361 agtttgattg ataatatgct gcgatcagaa caattagtaa aggctagtga agcagaaaat
     6421 aatgcgattt tattcaaacc aaagggtgac tccgttgaca atgacaactt ttcacctgat
     6481 gaggggaatg taatattagt aaataatcag ttttggtcga tttatcacaa gcagtttcaa
     6541 cctgatattc cgataaaaaa tcaaaaaaat aatgtcgaag taattattcc gcaaaagttt
     6601 catgcaatgc gtaatgaaat caatcaagcg tatcattcat ggtttgaatt tgtacaaaat
     6661 aaaaataata aagagaataa gttatcttta cagtttatca acaaaaatga ttatcgaatt
     6721 tttacatttg atgcacgaga tagtcgccat ttgtcattta tagaggcgcc aatcattgtg
     6781 aatgttcagg catcagattt atcgaatgat ttttattatg ccatgatcag tcaaggcggc
     6841 tatttattca aaaattatga cgcactggta aaaaatattg aaaagtatca tcttgatggg
     6901 gaaatcagtg gaataaccaa ttataaagat agcgtgatgg aaatgtatca tgaaaataat
     6961 ttgaaattaa cagtacttaa cttttcacaa atcattatcg caatcatttt aataattatt
     7021 attttatttg atgtgaaata ttattttgaa caacatcgaa aattactcgt aatcaaaaag
     7081 ctatatggtt attcaacatt aagagccaat taccaatact tattaataaa taatatagtt
     7141 gttgttttta ttggaatatt gacgaatgta attttacatt ctcactatat aatgatgata
     7201 tttgcaacga tccttgtcgt tcaaatatta ttgcagattt gcagcctata ctatcatggc
     7261 cgacgtttta atgaagttat caaggagttt taactatgaa atttatcatt gcaatattat
     7321 taggattaat cgtatccatt actattgctt ttacaatcat acatcatcct atactagatc
     7381 gttttaatcc tttcttaaaa acggagtata gttatgccaa agtgccaaaa ggtacgcaac
     7441 aatatgttaa tattacggct tatagtgaaa gaggggaaaa gcttgattat aaattaacat
     7501 ttaatggatt ttcacctagt agaacgtatg tcgaaataaa gcataaaggg caatatgtta
     7561 tatcgatcac atatgttgaa aaagaggata caccaaaaga ggtaagacaa gaatgataga
     7621 attaaaagat ttaaccatac aaaaaggtaa tacacatatt ttgaagaaac ttaatttgaa
     7681 atttcaatgt ggaaaatcat atgcacttat tggtaaaagt ggatgcggta aatctacatt
     7741 attaaatact attgctggac ttgaaaaaac agggaaacaa tatgtctatt ttaatggtca
     7801 attagaacaa tttaaatcta atttttacag agataaatta gggtatttat ttcaaaatta
     7861 tggattaata gataatctga cagtaaatga aaatttagat attggattag catataaaaa
     7921 aataagtaag aaagaaaaag aacaattaaa gatacgttat atagaacagt ttggtctgtc
     7981 aaacagtttg aaaagaaaag ttcatacgct aagtggaggt gaacaacaac gtgtcgcttt
     8041 aattagaatg atgttaaaag atccgattgt tatgttagct gatgaaccaa cgggtgcgtt
     8101 agatcctaaa acaggacaga tgattattca atcattattt gatttggtcg atgaaaataa
     8161 agtgctaatt ttagcaactc atgatatggc tattgcaaat caatgtgacg aaataataga
     8221 tttagaacag tatagaaaag tagcatctat gtgatatgca aaagataaaa ttaaaagcca
     8281 atcaattggg aatgttaatt tcaacactcc gtttgattgg cttttataat acgttgctat
     8341 gttatgaaag taacaacggc aaactgaaaa aaagcaatat aataatattt aatacgatta
     8401 gtaataaatg taattgattt ttcgttttaa tatagcaaaa aacaatgact aaagataaca
     8461 gcaatatcat gattgcacct gggaagaacc ataatgctga agctgcatac caaatcattg
     8521 gtaacgccat aataatgaaa gatataagac aagaaaggat accaataata ttataagttc
     8581 tagtcaaaat tacacctcca attttcattt taaaatgaca tgcgtaaaac aatcatttta
     8641 aatacatcat acaataaaat taaattgtat gatagatgaa tttgaaatta aattgaattg
     8701 gcttatcttt cgtaaacata attatgacgt aacaatcctt ataagtggaa cgagtgtaat
     8761 tataccatca gtcgtatcaa tcataaaatt taatctatgt tgttattgat ttttatatta
     8821 caaataccgt tctaacattg tgcaaaacat tattttactc aaataaaaga aattgaatat
     8881 tttaattatt gtatatatag ttgctctaaa tggttacgga gtataaaata tattttgtta
     8941 aatattttta ttaagaggtg cacaatggaa aaagggattg ttaactatga tgatgccaac
     9001 attttaaaat tagatatgaa ccaagtaagg tgtaataaat tagtagtcga cgatatattt
     9061 aaagattatg agcaaataaa gccaacgata gaaatagaaa agggaaatgc aattttgaaa
     9121 ttgaatggtt attttgttgc atctatttta gaaacgttga atttgaatag agttaaaaag
     9181 ttatatgttg atgaagatta ttattataca tataacgaat taattgttaa atatactgag
     9241 gtaaaggaat aataaatatg aaaaaagtat atatgatggt gcatgagtta gatgttaata
     9301 aaggtggcat gacctcttct atgttcaata gaagtaggga gttttatgat gctaatatat
     9361 cagctgatat agttactttt gattacaaag gtaattatga cgaaattatt aagaatttaa
     9421 aaaaacaagg taaaatggat tcaagaacaa aaatgtataa tgtgtttgag tattttaagc
     9481 aaatatccaa tagcaaacac tttaaatcta atagattatt atacaaacat atttctgata
     9541 gattgaaaaa tacaattgaa attgaagaaa gtaaaggtat ttcaagattt ttcgatatta
     9601 ctacaggaac atatattgct tacattagaa aaagtaaaac tgaaaaagtg atcgatttct
     9661 ttaaagataa tcaacgaata gaacgattta gttttaacaa caataaagtg catatgaaag
     9721 agacatttaa tatagataat aaggtgtgtt atcaagtatt ttatgatgaa aaaggatttc
     9781 catatatttc taggaatatc aatgcaagta acggcgcagt tggtaaaaca tatttaatag
     9841 tatgtaaaaa agagtttaaa aataatcttg cactatgtgt ttactactta gaaaaactta
     9901 tcaaagataa taagaatagt attatgattt gtgatggacc aggtagtttt cctaaaatgt
     9961 ttaatacaaa acataaaaat gctcaaaagt atggcgttat ccatgttaat catcatgaaa
    10021 attttgatga tagtggagca tttaagaaaa gtgagaaatt tattattgaa aatgctgata
    10081 acataaatgg tgttattgtg ttaacagatg cacaacgttt agatattctt caacaatttg
    10141 atgtgaaaaa tatatttact attagcaatt ttgtgaaaat acataaagca cctaagcaat
    10201 ttcagactga aaaaattgtt ggtcatattt ctagaatggt tccaactaaa agaatagatt
    10261 tacttattga tgttgctgaa ttagt
//
