LOCUS       NZ_CP034414            10327 bp    DNA     circular CON 27-MAY-2024
DEFINITION  Bacillus safensis strain BLXSC chromosome.
ACCESSION   NZ_CP034414
VERSION     NZ_CP034414.1
KEYWORDS    RefSeq.
SOURCE      Bacillus safensis
  ORGANISM  Bacillus safensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 3801264)
  AUTHORS   Saroj,D.B. and Gupta,A.K.
  TITLE     Full genome sequence for characterization of the strain BLXSC Safety
            evaluation of the strain through multidisciplinary approach
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3801264)
  AUTHORS   Saroj,D.B. and Gupta,A.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2018) Applied Microbiology Laboratory, Advanced
            Enzyme Technologies Limited, 5th Floor, A Wing, Sun Magnetica, LIC
            Service Road, Louiswadi, Thane, Maharashtra 400 604, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-NOV-2018
            Assembly Method                   :: MaSuRCA v. 3.2.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 355.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023524005.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 04:40:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,916
            CDSs (total)                      :: 3,803
            Genes (coding)                    :: 3,765
            CDSs (with protein)               :: 3,765
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 21 of 38
            Pseudo Genes (incomplete)         :: 20 of 38
            Pseudo Genes (internal stop)      :: 7 of 38
            Pseudo Genes (multiple problems)  :: 9 of 38
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:04:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2268875
            Orig. end                         :: 2279202
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP034414.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10327
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="8"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10327
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10327
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5327](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5327
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(266..562)
                     /locus_tag="EI692_RS11690"
                     /old_locus_tag="EI692_11775"
     CDS             complement(266..562)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356180.1"
                     /locus_tag="EI692_RS11690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EI692_11775"
                     /product="DUF3986 family protein"
                     /protein_id="WP_249856280.1"
                     /transl_table=11
                     /translation="MKVCTNERIHSLDESMHLHVGYYEQGCDYEGVFFKSLETGRWLLF
                     FDQKSYGLNLLKSYEEYDHLGLFIGEYPIEDDQIEEKGQQLFEHFLKENNIVG"
     gene            complement(572..988)
                     /locus_tag="EI692_RS11695"
                     /old_locus_tag="EI692_11780"
     CDS             complement(572..988)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012010659.1"
                     /locus_tag="EI692_RS11695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EI692_11780"
                     /product="hypothetical protein"
                     /protein_id="WP_249856281.1"
                     /transl_table=11
                     /translation="MFLYYEPTGAPIENPGEEFMKRIFFQEGEEYWKQGNGESSIEIEE
                     DWESESLVFFYTEPHGFFIIRYPDFLVPFQKVDKIETIEHLVAGSPLYVPSCSYVDRNQ
                     AYQIVQHFLTTKKVSDLVEWVELRNIDFQHDVKS"
     gene            complement(1024..1449)
                     /locus_tag="EI692_RS11700"
                     /old_locus_tag="EI692_11785"
     CDS             complement(1024..1449)
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017367646.1"
                     /locus_tag="EI692_RS11700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EI692_11785"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_249856282.1"
                     /transl_table=11
                     /translation="MKRVDVVSAIIYDEEENLLTVKNVKGYWELPGGAVEKGEHLQQAV
                     IREVKEETGYVVKVKELHSVREAFFQDKKHHALIITFFAEMIGGEMNILDPDHDIEEVK
                     WVSTQTFQKLNPKLYHMLKLNQQTQAFYDFEGNRTIG"
     gene            complement(1739..1930)
                     /locus_tag="EI692_RS11705"
                     /old_locus_tag="EI692_11795"
     CDS             complement(1739..1930)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017367648.1"
                     /locus_tag="EI692_RS11705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EI692_11795"
                     /product="hypothetical protein"
                     /protein_id="WP_048238943.1"
                     /transl_table=11
                     /translation="MVLMRLISIYMLTAFSLAFVINYVPGLMLPIYILAMIGLILCLGH
                     IALHYIRRIRYMLFDEKR"
     gene            complement(2038..3441)
                     /locus_tag="EI692_RS11710"
                     /old_locus_tag="EI692_11800"
     CDS             complement(2038..3441)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012010661.1"
                     /locus_tag="EI692_RS11710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EI692_11800"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_249856283.1"
                     /transl_table=11
                     /translation="MSIKWKVSLVLCGLAMIIGIAAFYHNQQASGTEKKKVEPDASYKE
                     VEIVTLVNDGRYLRYAVNYPVFHQKKLDQEMKRFAETALDKFKTSFSEASDVDEDHRYE
                     LNIDYEITHYAKQTAAIKFNTYELKGGVKGAHETKIFNFDFKKQQFLSIDDLFLSKKES
                     LQKLSQMTFEELKKDRSLAENEHLLKQGTSPKAEHYQYFAIKDRFIEFYFPAGQIAPDN
                     KGPQVLAIKKTLLKEMLKPEYINKEKNKNEKKEPIAKRKIVKLPKQRVFNPKQKAIALT
                     FDDGPNPSTTSKILKALKENKGHATFFVLGSRVQYYPDMLGEIRKGGNEIGNHTYNHPL
                     LTRLPLKQAIKQVEDTQQIIEKAGGFTPTHFRPPYGGTNGQINAAVNMKVTLWDVDPED
                     WKYRNSQYVANYILTHASSGETVLMHDIYDTSANAAVTIIKELTKQGYQLLTVSELEQW
                     KEARAKKVN"
     gene            complement(3601..4155)
                     /locus_tag="EI692_RS11715"
                     /old_locus_tag="EI692_11805"
     CDS             complement(3601..4155)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017358048.1"
                     /locus_tag="EI692_RS11715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EI692_11805"
                     /product="delta-aminolevulinic acid dehydratase"
                     /protein_id="WP_249856284.1"
                     /transl_table=11
                     /translation="MDQQAMRITLAAGPNSELESFALRAALENFGAKVDMHWIGRPNDL
                     IHVLSGRNRGEIDYLLLSFHGEEGAFIMPELGEDVYEDEEPRDTAFRADEIKQYAFLKG
                     IHVISTGCTLGEKNLADAFLDAGATSYTAPDDYIDGNAAFIFVLTMLYELMNNTKTVRA
                     AFDRARSIDEETKLFQLYMQK"
     gene            complement(4173..4739)
                     /locus_tag="EI692_RS11720"
                     /old_locus_tag="EI692_11810"
     CDS             complement(4173..4739)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010198845.1"
                     /locus_tag="EI692_RS11720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EI692_11810"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_249856285.1"
                     /transl_table=11
                     /translation="MIKISFQDKIRSIHHSFGGDFLNIRLLTPDDAEAYAALRLEALRL
                     NPEGFAMSYEEEPINIADKYKARFASANSMWTFGAFHHEQLVGAVTLIQESLQKLKHRA
                     NLTAMYVTPSARGEGTGQALMTKVLSFARERQDIEQIYLSVVTTNHSAKRLYRSVGFES
                     YAVEEKALKWTDTYADEEKMVLFLS"
     misc_feature    complement(4966..4982)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 26.19"
                     /tool="antismash"
     gene            5001..5327
                     /locus_tag="EI692_RS11725"
                     /old_locus_tag="EI692_11815"
     CDS             5001..5327
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IId"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017358050.1"
                     /locus_tag="EI692_RS11725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EI692_11815"
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="WP_249856286.1"
                     /sec_met_domain="Bacteriocin_IId (E-value: 8.3e-25,
                     bitscore: 84.5, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTETKNEIKLHVLFGALAIGFLMLALFSFSLQMLPVADLAKEFGI
                     PGSVAAVVLNVVEAGGAVTTIISILTAVGSGGLSLIAAAGKETIRQYLKNEIKKKGRKA
                     VIAW"
     misc_feature    5453..5473
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 24.81"
                     /tool="antismash"
     gene            6246..7166
                     /locus_tag="EI692_RS11730"
     CDS             6246..7166
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EI692_RS11730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_249856287.1"
                     /transl_table=11
                     /translation="MATLVMKTPVRWIPTGKEHDQSSKHFRDLFYWYFQALDKIQNSLF
                     QKNMLIQKDLKVLKEKRWLLSKEFFNYMFMSYEAFFYVGVMTGLISLIDHQALQIQLLF
                     TLNVMILANQTYEMRMLTPSIFSLSAEKKNIWLYELSQTSLMQFFLSKIQLFYCLFSIP
                     SLFLLFINLSIMSVYHLFDFQILIQLVALGIGFFIFPLIQLYLFPFTTKFNFIHEHEIG
                     TTNEEIEVIDKGQAFARNFLVLPFMYYMILTSFLPILQHSTSLISVLFALYFVIVSVIC
                     WVLCRKTIYKGIEYVEKNKMFYIGE"
     gene            7129..7668
                     /locus_tag="EI692_RS11735"
                     /old_locus_tag="EI692_11825"
     CDS             7129..7668
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342553.1"
                     /locus_tag="EI692_RS11735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EI692_11825"
                     /product="stage II sporulation protein M"
                     /protein_id="WP_223251007.1"
                     /transl_table=11
                     /translation="MLKKIRCFISENRLILRLGYLYAAIFIAAVLIGSLLPYDQIPYLK
                     KDEEMFFLSIFLNNLQVGMAILGIGAFTGGIMSAGILFYNGYIIGKLVQYLIVNHETQQ
                     LFTGLLPHAFIEVLGLILFSIVSSYPFIYLYRFIKGETIELKKLILTVLQLIFAAAVLL
                     FIASLLEQYVSHVHLP"
     gene            7690..8235
                     /locus_tag="EI692_RS11740"
                     /old_locus_tag="EI692_11830"
     CDS             7690..8235
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017358053.1"
                     /locus_tag="EI692_RS11740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EI692_11830"
                     /product="hypothetical protein"
                     /protein_id="WP_249856288.1"
                     /transl_table=11
                     /translation="MELLLRPKQFFNQNQSIKTIVGLVLLSLFVSTVFLTFFIIDLLVD
                     EPLSTGKQLASIVFIFLLTIPLYFILNFLSTVLTSIYMYFFHKAFILRKMYLVILVYNA
                     FLLLVNSAAIYCVMVLQLDHYFLLIQIVSFLFNLYLLRILYDGIIYYAEGSKKAALATV
                     ILYMMVTTAFVIGGFING"
     gene            8228..8896
                     /locus_tag="EI692_RS11745"
                     /old_locus_tag="EI692_11835"
     CDS             8228..8896
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017358054.1"
                     /locus_tag="EI692_RS11745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EI692_11835"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_144471160.1"
                     /transl_table=11
                     /translation="MAKLQVSQLSFRYETTEETSPLLDHVSFDLKENDRACIIGKNGSG
                     KSTLLKIIAGLLPSASESILLNEQHHPGTAQYKSLVAYIPDKPLVYDALTGFEHMELIQ
                     SLWKMKKSEKVLYKETFLSLCDAFDLTHALKMPVKQYSLGMRYKLFFNCMVARSPELII
                     LDEPFTSLDEASQIQSISLLKKEFSHRCVLFTSHQRYLYSELGNRFFQLDQGQLNEMER
                     "
     gene            complement(8936..9214)
                     /locus_tag="EI692_RS11750"
                     /old_locus_tag="EI692_11840"
     CDS             complement(8936..9214)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003216648.1"
                     /locus_tag="EI692_RS11750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EI692_11840"
                     /product="spore coat protein"
                     /protein_id="WP_048238948.1"
                     /transl_table=11
                     /translation="MSRNRLDDFFFGPRREETVVFPTRQIVKTRTNEQTIRKIHPTHIT
                     NVNKNIKRIENYYPVTESTHNEYIVEEYDCGSDIKNPCCRPVKRCKW"
     misc_feature    complement(9293..9306)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.28"
                     /tool="antismash"
     gene            9465..9677
                     /locus_tag="EI692_RS11755"
                     /old_locus_tag="EI692_11845"
     CDS             9465..9677
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017367657.1"
                     /locus_tag="EI692_RS11755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EI692_11845"
                     /product="hypothetical protein"
                     /protein_id="WP_095409082.1"
                     /transl_table=11
                     /translation="MINTIQPFERNKLDIALELTQLHLNQYQTNHPDQLSEIYAKYYAL
                     LEVLDLSSYDQLIDLLPNELKQTLE"
ORIGIN
        1 actttacgac atgaaatagt tgaattttta aagtctcatt tagatgaaaa acaagatcga
       61 atagtaacag ttccttggga tgaagacgaa tacactaaaa atgaaatatt acatcatata
      121 attgcccacg aaattcacca tatcggtcaa ctttctgttt gggcaagaga aaaagaatta
      181 acacctgtat ctgctaattt tatcggtaga aagttaaaat cagtacattt ttatgaaaag
      241 taaggtcagt taaagatcca ttcatttatc ccactatgtt gttttctttt aaaaaatgct
      301 caaataattg ctgacctttc tcttcgattt gatcatcttc aattggatat tccccaatga
      361 ataagcctaa atgatcgtat tcctcataac tctttaagag attcaacccg taactttttt
      421 gatcaaaaaa caataaccat cttcctgttt ctaaactctt aaaaaacacg ccttcataat
      481 cacaaccttg ttcataatat cctacatgta aatgcatact ctcatctaaa ctatgtattc
      541 tttcatttgt acacactttc accttactcc cctaactttt cacatcatgc tgaaaatcaa
      601 tattcctcaa ctcgacccat tcaacaagat cagatacctt ttttgtcgtc aaaaaatgtt
      661 gaacgatttg atacgcctga ttgcgatcga catagctgca cgatggcaca tacaatggtg
      721 atccagcgac caaatgctcg atggtttcaa tcttatctac cttttgaaaa gggactaaaa
      781 aatctggata tctgatgata aagaacccat gtggttcagt ataaaagaaa actagacttt
      841 cagattccca atcttcctct atctcaatgg aagattcccc gttaccttgt ttccaatact
      901 cttccccctc ctgaaagaag attcttttca tgaattcttc tccgggattt tctatcggag
      961 ctcctgtagg ctcatagtat aaaaacatca atatcgctcc ttcatgccta tatcaatttg
     1021 gatttaacca attgttctat ttccctcaaa atcataaaaa gcttgtgttt gttggtttaa
     1081 ttttagcatg tgatataact ttggatttag cttttgaaag gtttgagtgc tgacccattt
     1141 tacttcttca atatcatgat ccggatctag aatgttcatc tctcccccga tcatttccgc
     1201 aaagaatgta atgataagcg catgatgctt tttatcttga aagaatgctt ctcgaaccga
     1261 atgaagttcc tttactttga caacataccc tgtctcttct ttcacttcgc ggatgacagc
     1321 ctgctgcagg tgttcacctt tttccacagc accgcccgga agttcccaat atccctttac
     1381 attcttgact gttaatagat tttcttcctc gtcataaata atagctgaaa ccacgtctac
     1441 tcgtttcaaa tgaatccccc tcaacattct ccattcacat caattgccgg atatatgaca
     1501 cgaactgttc attttagaca ttcttattat actgtatcct tgatatatga tagtggaggt
     1561 catagcatgt ggatgatcat cattttacta attgtcctca taggatttac aggaaatatc
     1621 atttctcttt tgagacaact ccaagcgtca aacgagacaa tcatccagct gttaaaacat
     1681 caaaaaccga aaaaaacacc tcgctcaact agcaggaggt ttttcaatag gagaaatctt
     1741 atcgtttttc atcaaacaac atatatctaa ttcgccgaat ataatggagt gcaatatgac
     1801 cgagacataa aatgagccca atcatcgcaa gaatataaat gggcagcatc aaaccaggta
     1861 catagtttat aacaaatgcc aaactaaacg ctgtcagcat atatatgctg atcaacctca
     1921 tcaacaccat tcgaagccct ccctttctac aattacttta acatattttc ccttcattct
     1981 cataattgga ttaaaaaaag atacgaatcc tttaagactc gcatcttttt tacgtattca
     2041 attcaccttt ttcgcccgag cttccttcca ctgctcaagc tccgatactg ttagtaattg
     2101 atatccttgt ttggtcaatt ctttaatgat cgtgacagcc gcgtttgcag aggtgtcata
     2161 gatgtcgtgc attaagacgg tttctccgct agacgcatgt gtgagaatgt agttggcgac
     2221 gtattggctg tttcgatatt tccagtcttc aggatcaacg tcccagagcg taactttcat
     2281 gttgacagcc gcgttgatct gcccatttgt tccaccatat ggcggtctaa agtgagtcgg
     2341 tgtgaacccg cctgcttttt caatgatttg ctgtgtatct tctacttgtt tgatagcttg
     2401 ttttaaaggc agtcttgtga gtaaagggtg gttataagta tgatttccga tttcatttcc
     2461 accttttctg atctcaccta gcatatcggg ataatattga accctgcttc ctaatacaaa
     2521 aaatgtcgca tgtcctttgt tttcttttaa agcttttaag atttttgaag tcgttgatgg
     2581 atttggtcca tcatcaaatg taagggcaat ggctttttgt tttgggttga acactctctg
     2641 ttttggcagt tttacgatct ttctttttgc gatcggttct tttttctcgt ttttgttctt
     2701 ttctttattg atgtattctg gctttaacat ttctttaagc agtgttttct taatcgccag
     2761 cacttgaggg cctttattgt ctggtgcaat ttgccctgct ggaaagtaga attcaataaa
     2821 acgatctttg atggcaaaat attgataatg ctctgcctta ggagatgttc cttgtttgag
     2881 caaatgctcg ttttcagcaa gagagcgatc ttttttcaac tcctcaaatg tcatttgaga
     2941 cagtttttgc agactttctt tttttgataa aaatagatca tctatcgata aaaactgctg
     3001 tttcttgaaa tcaaaattga atatcttcgt ttcatgtgcg ccttttactc cgcctttcaa
     3061 ttcatatgta ttgaatttga tcgcagcggt ttgtttcgca taatgagtaa tctcataatc
     3121 tatgtttaat tcgtatcgat gatcttcatc gacatcgctt gcttcactaa atgatgtttt
     3181 gaatttgtcc agcgctgttt ctgcaaatct tttcatctct tgatctagct ttttttgatg
     3241 aaacacagga tagttcacag catatctcaa gtatctcccg tcattcacga gggtcacaat
     3301 ttcaacttct ttatagctcg catctggttc taccttcttc ttctctgtcc cgcttgcctg
     3361 ctgattatga taaaaagcag cgattccgat gatcatcgca agtccgcata ggacgagact
     3421 gactttccat ttaattgaca caatgattct cctctcgtga tgccgtcatg cctgaatgac
     3481 gtgtgtttgt aggatagact ttgcatgccc cctttttagt ttgcaaatca cacaattgtc
     3541 acattttcga cacattttta gagaggaata aaaaaaggac ttgatttctc agtcctttga
     3601 ttacttttgc atatatagtt gaaataattt cgtttcttca tcaatggaac gcgctcgatc
     3661 aaatgctgct ctgaccgtct ttgtattgtt catgagctcg tacagcatgg tcagcacaaa
     3721 gataaatgct gcattcccat cgatgtaatc gtccggtgcc gtataagatg tagctcctgc
     3781 atctaagaaa gcatctgcta gatttttctc tcctagtgtg cagcctgtag agatgacatg
     3841 aattcctttt aaaaaggcat actgcttgat ttcatccgct cgaaaagcag tatctctagg
     3901 ctcctcatct tcgtatacat cttcccctaa ttcaggcatg ataaatgctc cttcttcacc
     3961 gtgaaacgat aaaagaaggt aatctatttc tcctctattt ctaccagata acacatgaat
     4021 caggtcattt ggtcttccaa tccaatgcat atcgactttc gctccgaagt tttcaagtgc
     4081 tgcacgcagt gcaaaagatt ccagctcact attaggtcct gctgctaatg tgatgcgcat
     4141 ggcttgttga tccaccttct atctcctcca gactatgata aaaataaaac cattttttcc
     4201 tcatctgcat atgtatcagt ccattttagc gctttttctt cgacagcgta cgactcaaat
     4261 ccaacagagc gatagaggcg tttggctgag tgattagttg tcaccaccga taaatatatt
     4321 tgctcgatat cctgtcgttc tctcgcaaag gaaagaactt ttgtcatcaa ggcctggcct
     4381 gttccttctc cgcgtgcact aggtgttaca tacatcgctg tcagattcgc tcgatgcttt
     4441 aacttctgca aggattcttg gatgagcgtg actgccccca caagctgttc atgatgaaag
     4501 gcgccaaatg tccacataga attcgccgaa gcaaaacgcg ctttatattt atccgctata
     4561 ttgattggct cttcctcata gctcatcgca aagccctctg gatttaatct cagtgcttct
     4621 agtctgagtg ctgcatatgc ttcagcatca tctggtgtta acaatcgaat attcagaaaa
     4681 tcgcctccaa aagaatgatg gatacttcta attttatctt gaaaggagat tttgatcaaa
     4741 agatattggc ccctctgcac atatctcttt atctatatgc aaattagact cgcttcccca
     4801 cctgtaaaaa caaggtattt ccgatcatgt ttacgtgttt tattctagca aaaacgattc
     4861 ttttctcact tatcttttca ctttatttat attaatctat aactaattca tgaaaaaaat
     4921 gaaaaaaaag gattgcggtg aactaaaaga tatcttaaga tataatagta ataaaaatac
     4981 aaaaaaggga ggaatttgat atgacagaaa caaaaaacga aatcaaacta catgttcttt
     5041 ttggtgcttt agcaatagga ttccttatgc tagctctttt ctccttctcg ctacaaatgc
     5101 tgccagttgc tgatctagca aaagaatttg gtattccagg cagtgttgca gcagttgttc
     5161 tgaacgtggt tgaagcgggc ggggctgtta caacaattat ctccatttta actgctgtag
     5221 gatcaggtgg tctttctctc attgcagcgg ctggtaaaga gacaattcgc cagtacctta
     5281 aaaacgaaat taagaaaaaa ggccgtaaag ctgttattgc ttggtaattc aattttatga
     5341 cccaatagaa ggagactttg ttctccttct atttaaattt ttatgcaggt gagaagatgt
     5401 tatatccatt attatcacta cttctatcaa ttagaatgaa acaacaatat tcaaatctag
     5461 ctcagtttat aatacgcagg gttccatttt tcaaatcgtt ttcttctaaa caatacatat
     5521 tattttcttg catccttgat attttattcg gatgcctgat tgtattcgtt atttcttcat
     5581 atctttactc cccggtctat caaatagaat gggaaagtgc ttttgtgata tttttgttcc
     5641 ttttctctat tgtcaaagga attgaatctt atagaagaac acatattctt ccttttgaag
     5701 agttgataca gcttgcacct atttccgcga gacacctttt tcaaacgctt ttgttttctg
     5761 agttggtatg gctactattt acgagcttga gtacttattt gatctatttt ctctttcaat
     5821 atttcattgc tcaaacacat gactggatat tctggttgaa gcatctcaat gttatattga
     5881 caggcttctt actttttatg gtcagcaata agatatttgg tgcttacatc tataacattg
     5941 tcgtaaaaaa aattgggatc attcgtgtta tttttttcgc aagcatctct ttactttttt
     6001 tcttatttgg aagggttcta atatcgaaca ttattttccc tttttatcac accttccgac
     6061 aactttttca atcaatttct tcgcttgcag aagatgcgtt ttggattaca ctttcaaaca
     6121 acttgatgtc tttatatcag aataatcttc aacaaatcca tcatgcttta ttttcacagg
     6181 attcggtatt tctacaattt ctagcgcttt ttacttcacc tgtcacattt ctcgtggtga
     6241 tattagtggc aacactcgtc atgaagaccc ctgttcgctg gattccgact ggaaaagagc
     6301 atgatcaaag ctcaaaacat tttagagatt tattttattg gtactttcaa gcacttgata
     6361 aaattcagaa ctcgctgttt caaaagaata tgctgattca aaaagacctt aaggtgctaa
     6421 aagaaaaaag gtggctgctg tctaaagagt tttttaatta tatgttcatg tcgtatgaag
     6481 ccttcttcta tgtcggggtt atgaccggtt tgatctcgct tattgatcat caagccttgc
     6541 aaattcagct attattcaca ttaaatgtga tgattttagc taatcaaaca tatgaaatga
     6601 gaatgctgac cccttctata ttttctttga gtgctgaaaa aaagaatatt tggctctatg
     6661 agctttctca aacatctctt atgcaatttt tcttatcaaa aatccagtta ttttattgtt
     6721 tattttccat accgtcactc tttctactgt tcattaatct atcaatcatg agtgtatatc
     6781 acctttttga ttttcagata ctcattcaat tggtggcatt ggggattggc tttttcattt
     6841 ttccattgat tcagttgtat ttgtttccat tcacaacgaa attcaatttt attcacgagc
     6901 atgaaatagg gacaaccaat gaagaaatag aagttatcga taaaggacaa gcctttgcaa
     6961 ggaattttct cgtgttgcct ttcatgtact atatgatctt gacctctttt ctacctattt
     7021 tgcagcattc tacaagtctc ataagcgttc tgtttgcttt atattttgtg atcgtttcag
     7081 taatttgctg ggtgttatgc cgcaagacca tctataaagg gattgaatat gttgaaaaaa
     7141 ataagatgtt ttatatcgga gaatagactg attctacgcc tcggttacct ctacgctgct
     7201 atctttattg cagctgtgct aatcggaagc cttcttcctt atgatcagat tccttatctt
     7261 aaaaaagatg aagagatgtt ttttctatct attttcttaa acaatttaca agtaggaatg
     7321 gcgatattgg gcattggagc ttttacaggc ggcatcatgt ctgcaggcat tcttttttac
     7381 aacggctaca ttatcggcaa actcgttcag tatttgattg tcaatcacga aacgcagcag
     7441 ctattcacag gactactgcc tcatgctttc atagaagttc ttggattaat tctgttttca
     7501 atcgtcagct cttatccttt tatttatctt tatcgattta taaaaggaga aacaattgaa
     7561 ttaaaaaaac tgatcctaac tgtgcttcaa ctgatttttg cagcagccgt tctcttattc
     7621 atagcatctc ttttagagca atacgttagc catgtacatc taccatagaa gaaaggagaa
     7681 tgtggttcca tggagctact tctacgacct aagcaatttt tcaatcagaa tcaatcgatt
     7741 aaaaccattg taggccttgt cttactatca ttatttgtgt cgactgtttt tttaacattt
     7801 tttattattg atttgttagt agatgaacct ttatccactg ggaaacaact tgccagcatt
     7861 gtttttatct ttttgctgac aatcccgctt tatttcattt taaattttct cagtactgtc
     7921 ctaacttcta tttatatgta tttttttcat aaagctttta ttttacgcaa aatgtatctt
     7981 gttattttgg tttataatgc atttctttta ttagtgaata gtgcagcgat ctattgtgtg
     8041 atggtgttac aattagatca ctattttctc ttgattcaaa tagtttcctt cttattcaac
     8101 ttatatcttt tacgcatcct ttatgatgga attatttact atgctgaagg atcaaaaaaa
     8161 gctgccttag caactgtgat cttgtatatg atggtgacta ctgcgtttgt tattggagga
     8221 ttcatcaatg gctaaacttc aagtgtctca gctttctttt cgctatgaaa caacagaaga
     8281 gacttctccc cttctcgatc atgtgtcatt tgatctgaaa gaaaatgatc gagcttgtat
     8341 tattggaaag aacggcagcg gtaaatcgac acttttaaag atcatcgcag gtctgcttcc
     8401 ttcagcatca gaatcgattt tacttaacga gcagcaccat cctggaaccg ctcaatataa
     8461 aagtctggta gcgtacattc cagataaacc tttagtttat gatgcgctca ctggtttcga
     8521 gcatatggag cttatccagt ctttatggaa aatgaaaaag tcggaaaagg ttttatataa
     8581 agagaccttt ctatcgcttt gtgatgcatt tgatttaact catgccctaa aaatgcctgt
     8641 caaacaatat tcattgggca tgagatataa acttttcttt aattgtatgg tggcaagatc
     8701 tccagagctg atcattcttg acgagccttt tacctctctt gatgaagcct ctcaaataca
     8761 atcgatttct ttattaaaaa aagagttctc acatcgatgt gttttattta caagccatca
     8821 gcgctacctt tattcagaat taggaaatcg tttcttccag ctggatcagg gacaattaaa
     8881 tgaaatggaa cgttagactt acaaaaggca tcccataaaa ggatgccccc cttcattacc
     8941 acttacagcg cttcaccgga cggcagcaag ggtttttgat atcactgccg caatcgtatt
     9001 cttctacaat atactcgttg tgagtcgatt cagtaacagg atagtagttt tctattcttt
     9061 taatgttttt attgacgttt gtgatatgcg ttggatgaat ttttctgatc gtttgttcat
     9121 tcgttcttgt tttgacaatt tgacgcgtgg ggaaaacaac cgtttcttct ctgcgaggtc
     9181 caaagaagaa atcatctaat cgatttcggc tcatctcaac accccttcaa gttgtctttc
     9241 acttacactc tatcgtatgt cagaggcatc tttcgtgatt agatgcttgt ctatttttta
     9301 aaaaatccac tatcgacctc attttctctt tctttacaaa agggcaattt taacatatta
     9361 tacaaatgat atattgagaa gaaagaacat ccatgaaaac aaaacataag ctctttttat
     9421 tcactgcatt ttttctactc atcatcaaat aagaggtgaa cgatatgatc aacacgatac
     9481 agccattcga acgaaacaag ctagacattg cacttgaact aactcaattg catctgaatc
     9541 aatatcaaac aaatcatcct gatcagctga gcgaaattta cgcgaaatat tacgcgctgc
     9601 ttgaagtgct ggatttgtct tcctatgatc aattgattga tttactgcca aatgagctca
     9661 aacagacgct tgagtaaaaa aaccatctac ccaatgggat agatggtttt ctttgattta
     9721 tacacgtgca gccatatcgg tttcgatttg ctctaagctt cttccctttg tctcgactag
     9781 cacaaatcgg gtgaataaca gcccgattaa gcagatgaca ccaaatgagg agaaaatgac
     9841 accgaagctg aatttgtctg ccaggatagg gaacacgagt ccaacggcta acgatcccgt
     9901 ccagttaaag gcagatgaaa tacctgcgcc gatacctctg acagatagcg ggaagatttc
     9961 gccgatgatg atccaggtga tcggtgccca tgagaaggca tagcacaaaa taaagcagca
    10021 aagtgaaatc aatgtcaccc agtttaaaat tccttcacta acgccaagtg cacctaatgc
    10081 ggcgggagca aagaaagaaa gggccatgcc cgatccccct acggtgagaa tcgttcggcg
    10141 gtcgaagcgg tcaacaaatt gtaaaaagat cacagtcgtg acgacaaaaa tgacaccgac
    10201 gatgactgta aaacctgcgg cgacctgtgg cgcaagacca acattccgag cgatactcgt
    10261 tgcataatag acaatggagt tcgcgccttg aatttgctga agtgtcgcca tcccaacccc
    10321 gataaaa
//
