LOCUS       NZ_CP027059            10381 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Paenibacillus konkukensis strain SK3146 chromosome.
ACCESSION   NZ_CP027059
VERSION     NZ_CP027059.1
KEYWORDS    RefSeq.
SOURCE      Paenibacillus konkukensis
  ORGANISM  Paenibacillus konkukensis
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 7969064)
  AUTHORS   Jung,H.I., Park,S., Niu,K.M., Lee,S.W., Kothari,D., Yi,K.J. and
            Kim,S.K.
  TITLE     Complete genome sequence of Paenibacillus konkukensis sp. nov.
            SK3146 as a potential probiotic strain
  JOURNAL   J Anim Sci Technol 63 (3), 666-670 (2021)
   PUBMED   34189514
REFERENCE   2  (bases 1 to 7969064)
  AUTHORS   Kim,S.-K., Jung,H.-I. and Lee,S.-W.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2018) Department of Animal Science and Technology,
            Konkuk University, Neungdong-Ro, Seoul 140701, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-MAY-2016
            Assembly Method                   :: other v. HGAP
            Expected Final Version            :: yes
            Genome Coverage                   :: 7968964.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023523925.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 05:36:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,013
            CDSs (total)                      :: 6,868
            Genes (coding)                    :: 6,761
            CDSs (with protein)               :: 6,761
            Genes (RNA)                       :: 145
            rRNAs                             :: 13, 12, 12 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 12, 12 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 45 of 107
            Pseudo Genes (incomplete)         :: 72 of 107
            Pseudo Genes (internal stop)      :: 13 of 107
            Pseudo Genes (multiple problems)  :: 20 of 107
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:26:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3203959
            Orig. end                         :: 3214340
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP027059.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Soo-ki Kim.
            Genome not circularized.
            This genome has a base modification file available.
FEATURES             Location/Qualifiers
     region          1..10381
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="7"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10381
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10381
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5381](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5381)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            260..2128
                     /locus_tag="SK3146_RS13620"
                     /old_locus_tag="SK3146_02734"
     CDS             260..2128
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007130385.1"
                     /locus_tag="SK3146_RS13620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SK3146_02734"
                     /product="LTA synthase family protein"
                     /protein_id="WP_249865552.1"
                     /transl_table=11
                     /translation="MEASWVRIWRSPYIIITLLLWVKVALLRYFLFDEVIVSWWLLDLA
                     SLAVLSGCVELLFRYPAKKTAYWIVNAVMSLIFFAATLYFEHFGSVITYTSVLEIKQVL
                     QISSSVKATIRLADYLFFLDFAVFFLLWLVFRRSGAHILLAVALRKYAVIAVTAACLIV
                     CAVFIKLNLGIVNELVRANQLGLLNYEASVLANSIKGRKTTNFSTTSEALNVVSDYEAS
                     YGYNPNAGETPKYFGSQQGKNVIVVQLEAFQNFPIHLNFHGQEVTPVLNKLAAESYYFP
                     HIFQQIGQGNTSDAEFMSNTSIYPTGTIPMSTGYGDRVLPSLPRLLEAIGYEADTFHVN
                     EVHFWDRYKLYPALGFTHYYDKPYYNNDFFNSFGASDEELFRVGAEKLSELHKQGKPFY
                     AQFITVSSHFPFKIPNDRRKMTIPADLEGKQLGDYLLSVNYTDYAVGTFIDRLKAAGIW
                     DNTIFVAYGDHFGLQPEDNDPKELSEQLGMTYHPVISRFNIPLFIHVPGQTAPQVVNQV
                     GGQLDIMPTITNLLGISLQQQQFLHFGQDLLNMRHNVLGMRYYMPTGSFFNNIIMFQPG
                     KAFDDGTAVTLRTLAPANIQPYRSDFDYIMNLMKMSDDYVKLLPKR"
     gene            complement(2223..3410)
                     /locus_tag="SK3146_RS13625"
                     /old_locus_tag="SK3146_02735"
     CDS             complement(2223..3410)
                     /GO_function="GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010276030.1"
                     /locus_tag="SK3146_RS13625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SK3146_02735"
                     /product="KamA family radical SAM protein"
                     /protein_id="WP_249865553.1"
                     /transl_table=11
                     /translation="MPQPKYLTDIDKILEIPEQERKKLKQITEKFVFRVNDYYLNLIDW
                     RDPNDPIRKLVIPNEGELSEYGRWDASDEDTNYVVPGCQHKYKTTALLIVSEVCGAYCR
                     YCFRKRLFRNDVKEAMSNVDPGLQYIENHPEINNVLLTGGDSLILGTPKLRGILERLRA
                     IEHVQIIRLGSKIPVFNPMRIYEDEALLETIREFSTANKRIYVMAHINHPREITREAKL
                     GFEALHQAGAIVVNQTPVLRGINDDPAVLGELLDKLSWAGVTPYYFFINRPVAGNRDFV
                     LPLQEVYRIVEGAKARTSGLGKRIRLSMSHTSGKIEILAIENGKAYLKYHQSRDDEYGK
                     FMVLDCPEDAAWFDDLPGSERFWKRPKKRTDDVVSVNELPDMPRAQTQTRGVMQA"
     misc_feature    complement(3379..3392)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.7"
                     /tool="antismash"
     gene            complement(3470..3643)
                     /locus_tag="SK3146_RS13630"
     CDS             complement(3470..3643)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SK3146_RS13630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_249865554.1"
                     /transl_table=11
                     /translation="MELSFALNVSSKPAQTKLTQAYSYRVEGGSTGRRKQEQVSRLALS
                     DRSRRRICKRLP"
     misc_feature    complement(3663..3676)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 20.94"
                     /tool="antismash"
     misc_feature    3664..3682
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 22.1"
                     /tool="antismash"
     regulatory      complement(3823..3961)
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /regulatory_class="riboswitch"
     gene            complement(4000..4905)
                     /gene="thpD"
                     /locus_tag="SK3146_RS13635"
                     /old_locus_tag="SK3146_02736"
     CDS             complement(4000..4905)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010269269.1"
                     /locus_tag="SK3146_RS13635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SK3146_02736"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_249865555.1"
                     /sec_met_domain="PhyH (E-value: 1.2e-33, bitscore: 115.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSTHPASEVQHATDLYPSRISDTPSITERRDPVVYSAWSPNCSI
                     TEEQSEFYRRNGYLFIESFFSPSEVQGLKEELKRMWAASRDSADGEVIREPDSDEVRSI
                     FNVHRSSDVFKALSGHSRLKSITDYILGSETYIHQSRINFKPGFTGKEFYWHSDFETWH
                     VEDGMPRMRALSCSIALEDNYHFNGPLMVVPGSQMDYVSCVGRTPDNHYEKSLRRQQYG
                     VPDHDSLAELVQRYGIDTPVGKAGSIVLFDCNIMHGSNSNITPMPRSNVFIVYNSVENK
                     LQPPYSGQPPRPEFIAARED"
     misc_feature    complement(4926..4942)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 21.66"
                     /tool="antismash"
     gene            complement(5001..5381)
                     /locus_tag="SK3146_RS13640"
                     /old_locus_tag="SK3146_02737"
     CDS             complement(5001..5381)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536118.1"
                     /locus_tag="SK3146_RS13640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SK3146_02737"
                     /product="ectoine synthase"
                     /protein_id="WP_249865556.1"
                     /sec_met_domain="ectoine_synt (E-value: 4.5e-51, bitscore:
                     170.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVKHLEQLIHTVDDVDTQTWNSRRLLLRKDGVGFSLNDTIIKAG
                     TETLIWYQNHIEAVYCIEGEGEIEVIGGETYPISPGMLYVLDGHEKHYLRGKTQMRMLC
                     VFNPPLVGQEVHDENGTYPLLP"
     gene            complement(5426..6718)
                     /gene="ectB"
                     /locus_tag="SK3146_RS13645"
                     /old_locus_tag="SK3146_02738"
     CDS             complement(5426..6718)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (31-367). E-value: 3.6e-74.
                     Score: 241.7. Matches aSDomain:
                     nrpspksdomains_SK3146_RS13645_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679978.1"
                     /locus_tag="SK3146_RS13645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SK3146_02738"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_249865557.1"
                     /sec_met_domain="Aminotran_3 (E-value: 7.2e-79, bitscore:
                     264.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTLTLEKPSMHVFEEMESEVRSYCRSFTTVFDKAKNARLWDKNG
                     NAYIDFFAGAGALNYGHNNERIRGKLLDYMLKDGVTHSLDMATGAKERFLTRFNDVILK
                     PRGLSYKVMFPGPTGTNAVESALKIARKVTGRSTVICFTNAFHGMSLGSLAVTGNSFKR
                     KGAGVELSHSVFMPYDGYFGPDVDTVKYMEKLLDDPGSGVDLPAAVILETVQGEGGLNA
                     ASPAWLKSMEALCRSKGILLIVDDVQMGCGRTGSFFSFDDTGLEPDIVCLSKSIGGYGL
                     PMALTLLKPDIDQWSPGEHNGTFRGNNLAFVAAAEALQYWANDEFRKAIELKSRTVRAA
                     LERMAARHPELQGEIRGRGMIQGIAFAEDGLAERLCQAAFDKGLIMETSGPRSEVAKLM
                     PPLTIEDGLLEQGLQIMAEAMKELAEQAQGQ"
     aSDomain        complement(5618..6625)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SK3146_RS13645_Aminotran_3.1"
                     /evalue="3.60E-74"
                     /label="SK3146_RS13645_Aminotran_3.1"
                     /locus_tag="SK3146_RS13645"
                     /protein_end="367"
                     /protein_start="31"
                     /score="241.7"
                     /tool="antismash"
                     /translation="FDKAKNARLWDKNGNAYIDFFAGAGALNYGHNNERIRGKLLDYML
                     KDGVTHSLDMATGAKERFLTRFNDVILKPRGLSYKVMFPGPTGTNAVESALKIARKVTG
                     RSTVICFTNAFHGMSLGSLAVTGNSFKRKGAGVELSHSVFMPYDGYFGPDVDTVKYMEK
                     LLDDPGSGVDLPAAVILETVQGEGGLNAASPAWLKSMEALCRSKGILLIVDDVQMGCGR
                     TGSFFSFDDTGLEPDIVCLSKSIGGYGLPMALTLLKPDIDQWSPGEHNGTFRGNNLAFV
                     AAAEALQYWANDEFRKAIELKSRTVRAALERMAARHPELQGEIRGRGMIQGIAFA"
     misc_feature    complement(6705..6716)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.49"
                     /tool="antismash"
     gene            complement(6767..7273)
                     /gene="ectA"
                     /locus_tag="SK3146_RS13650"
                     /old_locus_tag="SK3146_02739"
     CDS             complement(6767..7273)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006212828.1"
                     /locus_tag="SK3146_RS13650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SK3146_02739"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_249865558.1"
                     /transl_table=11
                     /translation="MESPVIRYRHPVKEDGAAVWSLAKQTGALDVNSVYCYILLCDYFR
                     DSCIVAEDEAGIAGFVSAFRIPGKPDRLFVWQIGVAPDRQRRGIAKQLLRELFSSPCCE
                     GVRYAETTISPSNRASQALFLAVAEERGVEVQLTEGYPASMFSEGQQHEDEILFVLGPK
                     SAPGI"
     gene            complement(7291..8061)
                     /gene="ehuA"
                     /locus_tag="SK3146_RS13655"
                     /old_locus_tag="SK3146_02740"
     CDS             complement(7291..8061)
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010269278.1"
                     /locus_tag="SK3146_RS13655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SK3146_02740"
                     /product="ectoine/hydroxyectoine ABC transporter
                     ATP-binding protein EhuA"
                     /protein_id="WP_249865559.1"
                     /transl_table=11
                     /translation="MQPIVKYSQVTKSFGDLEVIKGIDLEMAPGEKLALIGPSGSGKTT
                     LGRMLMTLEEPTSGTIELDGEGLWHMPRKGAFVRADERHLHRMRGKVGMVFQHFNLFPH
                     MTVLRNVTEAPRKVLGLSREEAAERAVSMLGKVGLQDKLDVYPAQLSGGQKQRVAIARA
                     LVMRPMVMVFDEVTSALDPELVGEVLEVIREIAEEGSMAMMLITHEMDFAKDVADRVIF
                     GAEGRIVEQGPPEAIFENPQSERLQSFLKRFRSS"
     gene            complement(8091..8810)
                     /gene="ehuD"
                     /locus_tag="SK3146_RS13660"
                     /old_locus_tag="SK3146_02741"
     CDS             complement(8091..8810)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuD"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006212830.1"
                     /locus_tag="SK3146_RS13660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SK3146_02741"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="WP_249865560.1"
                     /transl_table=11
                     /translation="MWNWHYAMQILPELLRALQMTVLATCCGFALSCFGGLLLAMASAS
                     RWAVMSAVSRGVVSFIRNTPLLVQIFFLYYTLPKVANISLSAFAVGIIGLGLHYSTYMS
                     EVYRSGIEAVPKGQWEAAVALNFPKAKTWMTVILPQAIPPVIPVMGNYLLVMFKETPVL
                     SAITLVEMLLTAKNIVSESYRVFEPYTMVGVLFLIISYPASLLIRALEKRLTLQPGQRP
                     ESGRGQAGRSAKKAVGS"
     gene            complement(8817..9470)
                     /gene="ehuC"
                     /locus_tag="SK3146_RS13665"
                     /old_locus_tag="SK3146_02742"
     CDS             complement(8817..9470)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuC"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015737576.1"
                     /locus_tag="SK3146_RS13665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SK3146_02742"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="WP_249865561.1"
                     /transl_table=11
                     /translation="MVTSWTQFIPLLAEGAKVTIAIAFLSSAVAMAVSLLIGLCRLSRF
                     RAVRAVSAVYVEFFRGSSLLIQLFWIYFALPFIGIELPKFAAAVVAFGLNYGAYGSEIV
                     RSSIQAIPKGQWEACTALNMKPWQTMKTIILPQAFLRMLPPFGNLLVELIKGTSLVYFI
                     TLADLTYQAMVLRNNYISWSPVIMVLLLLMYFAIASLVSMAVRLAERKMAAGRM"
     gene            complement(9472..10365)
                     /gene="ehuB"
                     /locus_tag="SK3146_RS13670"
                     /old_locus_tag="SK3146_02743"
     CDS             complement(9472..10365)
                     /GO_component="GO:0009897 - external side of plasma
                     membrane [Evidence IEA]"
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033294 - ectoine binding [Evidence IEA]"
                     /GO_function="GO:0033295 - hydroxyectoine binding [Evidence
                     IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119075.1"
                     /locus_tag="SK3146_RS13670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SK3146_02743"
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="WP_249865562.1"
                     /transl_table=11
                     /translation="MVLFLTLLTSVVGCGSAGQAGAGDGNAGKQASEAGNDTLAQAKQK
                     GSVTVGFANEKPYAYKTSDGQLTGEAVEIARVVLGKLGINEMKGELTEFGSLIGGLQAK
                     RFDMITAGMFINPQRCQAVLFANPEYSIGEAIAVQEGNPHKLRSYQDIANNKEAKVAVM
                     TGAIEIGYLEKSGVAKEQMVLVPDQASALNALQANRAQAVTMTGPALQAMLDSANDSKL
                     ERVADFEQPLIDSKSVRGYGATAFRPEDKSFQEAFNAELKKLKDSGELLEILQKFGFTE
                     QELPGDMTSEALCKEQ"
ORIGIN
        1 ccgcaatgtt taatatccgc ttatttggta aatgaatgta tagaagcatg agaagcgtgg
       61 tgaaaaaaag agcggagggg ccatttgaag ctggaaaagc gttagcgctc gcctttgaaa
      121 acggattttg tccatagaaa aaggagacga cactccaaaa atccggtttc aacagttatt
      181 ggaagaccaa atgggcgccg cagcggactt tttcatctcg cttttagata gaacgttcaa
      241 aacaaaatgg gggtctataa tggaagcaag ctgggttcgg atatggagaa gtccttatat
      301 tatcattact ttgctgctgt gggtcaaggt cgccctgctg agatattttt tattcgacga
      361 agtgatcgtt tcctggtggc tgctcgatct cgccagtctt gccgtgttgt caggatgcgt
      421 cgagctgtta ttccgttatc cggccaagaa aaccgcctac tggattgtta atgcggtgat
      481 gtcgctgatc ttttttgccg ctacgctcta tttcgagcat ttcggctccg ttattaccta
      541 tacctccgtt ttggagatca agcaagtttt gcagatttcc tccagcgtca aagcgacgat
      601 ccggcttgcg gattatttat ttttccttga ttttgccgtg ttttttttgt tatggcttgt
      661 ttttagacgc agcggggctc atatcctgct tgccgtcgct ttgcgcaagt atgcggtcat
      721 cgccgtaacg gccgcctgcc tcatcgtctg cgccgtcttc atcaagctga acctcggcat
      781 cgtcaacgag ctggttcgcg ccaatcagtt ggggctgctc aactatgaag cgtcggtttt
      841 ggccaatagc attaaaggca gaaaaacgac caacttcagc accacatcgg aagctttgaa
      901 tgtcgtaagc gattatgaag cgagctacgg atacaatccg aacgccggag aaacgccaaa
      961 atatttcggc tctcagcagg ggaaaaatgt gatcgtcgtg cagttggaag cgttccagaa
     1021 cttcccgatc catcttaact tccacggtca ggaggttacg cccgtattga acaagctggc
     1081 tgccgaaagc tactatttcc ctcacatttt ccagcagatc ggccaaggga atacgtccga
     1141 tgccgaattc atgtccaaca cgtcgattta tccgacaggg acgattccga tgtcgaccgg
     1201 ttacggcgac cgcgtgctgc ccagtttgcc ccgtctgctc gaagcgatcg gctatgaagc
     1261 cgatacgttt cacgtaaacg aggttcattt ctgggaccgg tataagctgt atccggcgct
     1321 cggcttcacc cattactacg acaagcccta ttacaacaac gacttcttca actccttcgg
     1381 agcatcggac gaggaattgt tccgtgtcgg ggcagaaaag ctaagcgagc tgcacaagca
     1441 gggcaaaccg ttctatgccc agtttattac ggtatccagc cactttccgt tcaagattcc
     1501 gaacgatcgc cgcaaaatga cgatcccggc cgatctcgaa ggcaaacagc tcggcgatta
     1561 tttgctgtcc gtcaattata ccgactatgc cgtcggcacg tttatcgacc gtctgaaggc
     1621 ggccggcata tgggataaca cgatattcgt cgcttacggc gatcatttcg gcctgcagcc
     1681 ggaggacaac gatccgaagg agctgagcga gcagctcgga atgacgtacc atccggttat
     1741 ctcccgcttc aacatcccgc tgttcatcca tgttcccggg caaacggccc cccaagtggt
     1801 gaaccaggtg ggcgggcagc tcgacattat gccgacgata accaacctgc tcggcatttc
     1861 gctgcagcag cagcaatttc ttcatttcgg gcaggatttg ctgaatatgc gccataatgt
     1921 gctcgggatg agatattata tgccgacagg ctccttcttc aacaacatca ttatgttcca
     1981 accgggcaag gcattcgacg acggtacggc cgtcacgctt cgcacgctgg ctccggccaa
     2041 cattcagccg taccgaagcg acttcgacta tattatgaac ttgatgaaaa tgtccgatga
     2101 ctacgtgaag ctgctgccca aacgatgatc cgtccgggat tctcgcttct ctgtaagcgc
     2161 ctgcggctgt gcggcaggag gagagggccg tcccgccatg accgcggcgc gggcaggaag
     2221 ggttacgctt gcataacgcc gcgcgtctgc gtttgcgccc gcggcatatc gggaagctcg
     2281 ttgaccgata cgacgtcgtc ggtccgtttc ttcggacgct tccagaagcg ttcgctgccg
     2341 ggaagatcat caaaccaagc ggcgtcttcg gggcaatcga gcaccataaa tttgccgtat
     2401 tcatcgtccc gcgactgatg gtatttgaga taagccttgc cgttctcgat agccaaaatt
     2461 tcgattttgc ccgaagtgtg gctcatggac aggcggattc gtttgccgag gccggaagtt
     2521 ctggccttgg cgccttctac gattcggtac acttcctgca gcggcagcac gaaatcgcgg
     2581 ttgccggcaa cggggcggtt gatgaagaaa taatacgggg ttacgccggc ccaggacagc
     2641 ttgtcgagca attcgccgag cacggccggg tcgtcattga ttccgcgcaa gaccggcgtc
     2701 tggttaacga cgatggctcc tgcctgatgc agcgcctcga agccgagctt cgcctcgcgc
     2761 gtaatttctc gcggatgatt gatgtgcgcc atcacataaa tgcgcttgtt ggcggtggaa
     2821 aactcgcgga tcgtctccag aagagcctcg tcctcgtaaa tccgcatcgg attaaacacc
     2881 ggaatcttcg agccgagccg gatgatctga acatgctcga tcgctctaag ccgctccagt
     2941 ataccgcgca acttgggcgt tccgagaatc agactgtccc ctccggtcag aagcacgtta
     3001 ttgatttcgg ggtggttctc gatatattga agcccgggat cgacgttcga catcgcttcc
     3061 ttgacatcgt tgcggaacaa tcttttgcgg aaacagtaac ggcagtacgc cccgcacacc
     3121 tcggatacga tgagcagcgc cgtcgtcttg tatttatgct gacagcccgg caccacatag
     3181 ttggtatctt cgtcggacgc atcccagcgc ccgtattcgg aaagctcgcc ttcattgggg
     3241 atgaccagct tgcggatcgg atcgttggga tctctccaat cgatcaggtt caagtaataa
     3301 tcgtttacgc gaaacacgaa cttctccgtt atttgcttaa gcttttttct ctcctgctcg
     3361 ggaatctcca ggattttgtc gatatcggtc aaatatttcg gctgcggcat agagtatcct
     3421 ccttgaaatg acagattggt tatggggaat cggcgtcatt agcggaatat caaggtaaac
     3481 gcttgcatat ccttctgcgg ctccggtccg ataaagccag tctgctaacc tgctcctgct
     3541 tacgcctccc tgtactccct ccttccacgc ggtatgaata agcttgcgtc agcttcgtct
     3601 gcgccggctt gctgcttaca ttcagcgcaa acgataattc catgaatgaa cttaaaagtt
     3661 aatttatgta taaaattaca ttcaaaaaag acccgtgctc cattcggaac atgcaagccg
     3721 taagcgctgc atggttctcc tcctggctct agcctcgggt cctcatatgc aaaaagaccc
     3781 gatttccacg ggtcaatgaa tgcaatttca tgcataaata ataacttgac ccatccactg
     3841 ctgacgaggt tagctgccgg gctcgggaaa gatggttccc tacaatcgtt actgattcgc
     3901 cccaaataat ggttcccccg cttcccggtt ccataaaccg gaaattaagc gtagtactat
     3961 catacactac gaaagtggat gagtcaaatt ggatcgtgcc tagtcttcgc gggcagcgat
     4021 aaattccggt cgcggcggct gtcccgagta tggcggctgc agcttgttct ccacgctgtt
     4081 atagacgatg aagacattgc ttctaggcat cggcgtaatg ttgctgttcg agccgtgcat
     4141 aatgttgcaa tcgaacaaaa cgatcgatcc tgccttgcct accggcgtat cgatgccgta
     4201 gcgctgcacg agctcagcca aactatcgtg gtcgggcacg ccgtactgct gccggcgcag
     4261 cgatttctca taatgattgt ccggcgtccg tccgacgcag gagacgtaat ccatctgcga
     4321 gccgggcacg accatcagcg ggccgttaaa atggtaattg tcctccagcg cgatggagca
     4381 gctgagcgcc ctcattctcg gcataccgtc ctccacatgc catgtttcga aatcggaatg
     4441 ccaataaaac tctttgccgg taaaacccgg cttaaagttg atccgcgatt gatggatgta
     4501 cgtctcgctt cccaaaatgt agtcggtgat gctcttcagc cgggaatggc ccgagagagc
     4561 cttgaacaca tcgctgctgc ggtgaacgtt aaagatcgat cttacctcgt cgctgtccgg
     4621 ctcgcgtatg acctctccgt cggccgagtc acggcttgcc gcccacatcc gcttcagctc
     4681 ctccttcagc ccttgcacct cgctcgggga aaagaacgat tcgataaaca agtagccgtt
     4741 ccggcggtaa aattccgact gctcctcggt gatggagcaa ttgggcgacc atgcggagta
     4801 gacgacgggg tcccggcgtt ccgtaatcga cggcgtatcg gagatccgtg aaggatacaa
     4861 atccgttgcg tgctggactt cgcttgcagg atgcgtgctt ttcataagat attcctcctc
     4921 gcaggtttta gtaaaattag atcaagctta gaccggcatc gggtttaagc gggttaagcg
     4981 ggttaagcgg gttaagcggg ttaaggcagc aaaggatacg taccgttctc atcgtgcact
     5041 tcctgcccga caagcggggg attgaacacg cagagcatgc gcatctgcgt cttgccgcgc
     5101 aaataatgct tctcatgccc gtcgagcaca taaagcatgc ccggactgat cggatatgtc
     5161 tctcctccga tgacctcgat ctcgccttcg ccttcgatgc agtacaccgc ctcaatatgg
     5221 ttctggtacc agatcagcgt ctccgtcccc gctttgatga tcgtgtcgtt cagggagaag
     5281 ccgacgccgt ccttgcgcag cagcagtctt ctcgaattcc atgtctgcgt atccacatcg
     5341 tcgaccgtat gaatcagttg ctccaaatgc ttcacaatca tgatgatccg ctccttttat
     5401 ttcgaataaa ttaccgggac aaggattact gtccctgcgc ctgttcagcc agttctttca
     5461 tggcttccgc cataatctgc agcccttgct ccagcagccc gtcttcaatg gtcagcggag
     5521 gcatcagctt ggcgacctcg ctgcgcggtc ccgaagtttc catgatcaag cccttatcga
     5581 atgccgcttg gcataaccgc tcggccaagc cgtcttccgc gaacgcaatg ccctgaatca
     5641 tccctctgcc gcgaatttcc ccctgcagct cgggatgacg ggctgccatc cgctccagcg
     5701 ccgcccttac ggttcggctt ttaagctcga tcgccttcct gaactcgtcg ttcgcccaat
     5761 actgcagcgc ctcggcggcc gccacaaaag cgagattgtt cccgcggaag gtgccgttat
     5821 gctcgccggg cgaccattgg tcgatgtccg gcttcagcag cgtaagcgcc atcggcagac
     5881 cgtatccgcc gatcgacttg gacaagcaga cgatatccgg ctccaatccg gtatcgtcga
     5941 agctgaagaa ggaaccggtc cggccgcagc ccatctgaac gtcgtcgacg atcagcagga
     6001 tgcctttgct tcggcacaag gcttccatac ttttgagcca agccgggctc gccgcattca
     6061 gtccgccttc gccctgaacc gtctccagga tgacggcggc cggcaggtcg acgccgcttc
     6121 ccggatcgtc cagcagcttc tccatatact tcacggtgtc gacatccggg ccgaagtagc
     6181 cgtcatacgg cataaagaca gagtggctca gctccactcc cgcgcccttg cgtttgaatg
     6241 agttgccggt gacggccaag gaaccgaggc tcatgccgtg gaaggcgttc gtaaagcaga
     6301 tgaccgtgga ccgccccgtt actttccgcg cgatcttcag cgcgctctct accgcgttcg
     6361 ttccggtcgg acccgggaac ataaccttat agctcaaccc ccggggcttc aaaataacgt
     6421 cgttaaatct cgttaaaaac cgctctttgg cgcctgtcgc catatccaaa ctatgcgtga
     6481 cgccgtcctt cagcatataa tcaagcagtt tgccgcgaat gcgttcattg ttatgaccgt
     6541 aattcaacgc tcccgctccg gcaaaaaaat cgatatatgc attgccgttt ttatcccata
     6601 gcctggcgtt tttggctttg tcgaaaaccg ttgtaaagct tcggcagtaa ctgcgaacct
     6661 cggattccat ctcttcgaat acgtgcatag agggcttttc cagcgttagc gttttcatcg
     6721 gttttcctcc ctgtgtgcgg ttctttttat cactgtgaat ctgcggctat atgcccgggg
     6781 ctgatttcgg ccccaatacg aacaatattt catcctcgtg ctgctgccct tcggagaaca
     6841 tcgaagccgg atatccttcg gttagctgaa cctcgactcc ccgttcctca gcaacggcca
     6901 gaaacagcgc ctgcgaagcc cgattcgagg ggctgatcgt cgtctccgca tagcggacgc
     6961 cttcgcagca gggcgatgag aacagctccc gcagcagctg tttggcaatg cctcttctct
     7021 gccggtccgg cgcgacgccg atctgccata cgaacaagcg gtccggcttg ccgggaatgc
     7081 gaaatgcgga cacgaagccc gcgatgcccg cttcatcctc ggcgacgatg cagctgtccc
     7141 tgaagtaatc gcacagcaaa atgtagcagt agacggaatt gacgtcgagc gcccccgttt
     7201 gcttcgcgag gctccacacc gccgccccgt cttccttgac cgggtggcgg taacgtatga
     7261 caggcgattc catgaagcct ctccctccct tcaagacgaa cggaatcgtt tgagaaacga
     7321 ttgcagccgt tcgctttgcg gattttcaaa aatcgcctcc ggaggacctt gttcgacgat
     7381 ccttccttcg gctccgaaga tgaccctgtc ggcgacatcc ttggcaaaat ccatctcatg
     7441 cgtgatcagc atcatggcca tgctgccttc ctcggcgatt tcgcgaatga cttcgagcac
     7501 ttcgcccacc agctccgggt ccagcgccga ggtcacctcg tcgaacacca tcaccatcgg
     7561 ccgcatgacc aaagctctgg cgatcgccac gcgctgcttc tgtccgccgg acaactgggc
     7621 cggatacacg tccagcttat cctgcaagcc gaccttgccc agcatgctga ccgcccgctc
     7681 ggccgcctct tccctggaca gccctagcac cttgcggggc gcttccgtca cgttgcggag
     7741 caccgtcata tgcgggaaca ggttaaaatg ctggaacacc atgccgacct tcccgcgcat
     7801 ccggtgcaga tgccgttcat ccgcccgcac gaatgccccc ttccggggca tatgccacaa
     7861 tccttcgccg tccagctcga tcgtgcccga ggtcggttcc tccaaggtca tcagcatccg
     7921 cccgagcgtc gtctttcccg aaccgctcgg accgatcagc gccagcttct cgcccggcgc
     7981 catctccaga tcgatgcctt tgatcacttc gagatcgccg aacgatttgg ttacttgcga
     8041 atatttcact attggctgca tgctctgccc ccttcgttgt cctagctact ttacgagccg
     8101 accgccttct tggcgctcct cccggcctgc ccccgtccgc tctccggccg ctgccccggc
     8161 tgaagcgtaa gccgcttctc caaggcgcga atgagcagcg aggccgggta gctaatgatc
     8221 aggaacaaga cgccgaccat cgtgtaaggc tcgaatacgc gatacgattc ggagacgata
     8281 tttttggcgg tcagcagcat ttcgaccaac gtaatcgcag acaaaaccgg ggtttccttg
     8341 aacattacca agagataatt ccccatcacg ggaatgacgg gcggtatcgc ttgcggcaaa
     8401 atgaccgtca tccaagtttt cgccttcgga aaattcaaag cgaccgccgc ctcccactgc
     8461 cctttgggca ccgcctcgat cccggagcgg tacacctcgg acatgtacgt gctgtaatga
     8521 agcccaagtc caataatccc tacggcaaaa gcagacaatg agatgtttgc gactttgggc
     8581 agcgtataat acaggaaaaa aatttgcacg agcagcggcg tgttgcggat aaagctcacc
     8641 actccccgcg agaccgcgga catcacggcc cagcgggagg ccgaggccat tgccagcagc
     8701 agcccgccga agcaggagag cgcgaagccg cagcatgtcg cgagcaccgt catctgcagc
     8761 gctcgcagca gctcgggcaa tatttgcatt gcatagtgcc agttccacat gcttacttac
     8821 atccttcccg ccgccatttt acgctcggcc agcctaaccg ccatcgatac aagcgaggca
     8881 atggcaaaat acatcagaag cagcagcacc ataatgaccg gcgaccagga gatgtagttg
     8941 tttcgcagca ccatagcctg atacgttaag tcggccaatg tgatgaagta gacgagcgat
     9001 gtgcctttga tcagctcgac gagcaaattg ccgaacggag gcagcattct tagaaacgcc
     9061 tgcggcaaaa taatcgtttt catcgtctgc catggcttca tattcaaggc cgtgcaggcc
     9121 tcccattgcc ccttgggaat cgcctgaatc gagcttctga cgatttcgga gccgtacgcg
     9181 ccgtagttca gcccgaaggc gacgacggcc gcagcgaatt tcggcagttc aattccgatg
     9241 aacggaagcg cgaaataaat ccagaacagc tgaatgagca gcgagcttcc ccggaaaaat
     9301 tccacataca ccgcagatac cgctcttacc gccctaaacc tggacagtct gcacaagccg
     9361 atcagcagcg agaccgccat cgcaaccgcc gaggagagga aggcgatcgc aatcgtcacc
     9421 ttcgctcctt ccgccagcag cgggatgaac tgcgtccacg aagttaccat gctactgctc
     9481 cttgcagagc gcttcggatg tcatgtcgcc gggaagctcc tgttccgtaa atccgaattt
     9541 ttgcaaaatt tcgagcagct ctccggaatc cttcagcttc ttcagctcgg cgttaaacgc
     9601 ttcctggaag ctcttgtctt cgggacggaa cgccgttgcg ccgtatcctc tgacactctt
     9661 ggaatcgata agcggctgct cgaaatcggc gacgcgttcg agcttgctgt cattggccga
     9721 atccagcatc gcctgcagcg caggcccggt catcgttacc gcttgcgccc ggttggcttg
     9781 aagagcgttc aaagccgacg cctgatcggg cacgagcacc atctgctctt tggcgacgcc
     9841 cgatttttcc aaataaccga tctcgatcgc accggtcata acggccactt tggcctcttt
     9901 gttattggcg atgtcttgat aactgcggag cttgtgggga tttccttcct gaaccgcgat
     9961 agcttctccg atactgtact ccggattagc gaagagaacc gcttggcagc gctgaggatt
    10021 aataaacatg ccggccgtaa tcatgtcgaa ccgtttggct tgaaggcccc cgattaatga
    10081 tccgaattcc gttaattcgc ctttcatttc gttgataccg agtttgccca gaactacccg
    10141 ggcaatttct accgcttctc ccgtcaattg accgtcggat gtcttatagg cgtacggctt
    10201 ctcgttagcg aagcccaccg tcaccgagcc tttctgtttg gcttgagcca acgtatcgtt
    10261 gcccgcttcg gacgcctgtt tgcctgcatt gccgtctccg gcgcccgctt gcccggcgct
    10321 tccgcagccg acgactgatg tgagaagcgt tagaaacagc accatcgcta gcccccttct
    10381 c
//
