LOCUS       NZ_CP027059            11931 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Paenibacillus konkukensis strain SK3146 chromosome.
ACCESSION   NZ_CP027059
VERSION     NZ_CP027059.1
KEYWORDS    RefSeq.
SOURCE      Paenibacillus konkukensis
  ORGANISM  Paenibacillus konkukensis
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 7969064)
  AUTHORS   Jung,H.I., Park,S., Niu,K.M., Lee,S.W., Kothari,D., Yi,K.J. and
            Kim,S.K.
  TITLE     Complete genome sequence of Paenibacillus konkukensis sp. nov.
            SK3146 as a potential probiotic strain
  JOURNAL   J Anim Sci Technol 63 (3), 666-670 (2021)
   PUBMED   34189514
REFERENCE   2  (bases 1 to 7969064)
  AUTHORS   Kim,S.-K., Jung,H.-I. and Lee,S.-W.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2018) Department of Animal Science and Technology,
            Konkuk University, Neungdong-Ro, Seoul 140701, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-MAY-2016
            Assembly Method                   :: other v. HGAP
            Expected Final Version            :: yes
            Genome Coverage                   :: 7968964.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023523925.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 05:36:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,013
            CDSs (total)                      :: 6,868
            Genes (coding)                    :: 6,761
            CDSs (with protein)               :: 6,761
            Genes (RNA)                       :: 145
            rRNAs                             :: 13, 12, 12 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 12, 12 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 45 of 107
            Pseudo Genes (incomplete)         :: 72 of 107
            Pseudo Genes (internal stop)      :: 13 of 107
            Pseudo Genes (multiple problems)  :: 20 of 107
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:26:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 262458
            Orig. end                         :: 274389
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP027059.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Soo-ki Kim.
            Genome not circularized.
            This genome has a base modification file available.
FEATURES             Location/Qualifiers
     region          1..11931
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11931
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11931
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6931](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6931)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(5..18)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 20.3"
                     /tool="antismash"
     misc_feature    580..596
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 21.78"
                     /tool="antismash"
     gene            689..1429
                     /locus_tag="SK3146_RS01140"
                     /old_locus_tag="SK3146_00224"
     CDS             689..1429
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1260: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /locus_tag="SK3146_RS01140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SK3146_00224"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_249863332.1"
                     /transl_table=11
                     /translation="MKSQVLSVGFGSKLSPSKVLPHTHDYWQLEIATEGVITCNLLGEM
                     RSLEIGDMLLVPPGVEHGFGYNEPPVAWISIKFERETGDLPHWTGIIHSNMITRKLISA
                     FKTAIHASPYMQYEKVFVGGFLETILHYVHSGEFREINDSSELLVKRMMDSIYKRNGKS
                     MTIYELAEELSYSRSHLSKKFKENTGESLKSFIDHIRIQKIEELLRYREQSISEIAADL
                     GFHDVFSFSKFFKKHTGKSPRQYK"
     gene            complement(1486..4080)
                     /gene="ppsA"
                     /locus_tag="SK3146_RS01145"
                     /old_locus_tag="SK3146_00225"
     CDS             complement(1486..4080)
                     /EC_number="2.7.9.2"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /gene="ppsA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PPDK_N"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003182498.1"
                     /locus_tag="SK3146_RS01145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SK3146_00225"
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="WP_249863333.1"
                     /sec_met_domain="PPDK_N (E-value: 1.9e-93, bitscore: 311.9,
                     seeds: 105, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHSLVLDFQEMEKTQLSLVGGKGFHLGKLSEIEGIRVPEGFCVTT
                     AAYQQAIEPNETYHALLHRLTTLQAEDRESIGEISGKIRQVIIEAEMPSDVTEAVSRYL
                     SRFGEEHAYAVRSSATAEDLPHASFAGQQDTYLNIIGLDAILQHIRKCWASLYTDRAVI
                     YRMQNGFDHSQAYVSVIVQRMVFPQASGILFTADPMTGNRKLLTIDAGFGLGEALVSGL
                     VSADVYKVQDETIVDKRIAAKTLAIYGREEGGTATKQIDPDRQKTPALTERQIVQLAHI
                     GRQIEAYFGQPQDIEWCLDQDAFYILQSRPITTLYPIPEANDGDNHVYLSVGHQQMMTD
                     PIKPLGLSFFLLITPAPMRKAGGRLFIDVAPMLATPAGREALLKNLGSDPLIKGALVTI
                     IERDFIKLLPNDPTAPIPNRMNTDMPTPFENGPAIVSDLIKRSQASIEELQQNIQTKSG
                     ADLFDFIREDIQQLKKILFDPQSSAVFMSAINASAWINENMNKWLGEKNAADTLSQSVP
                     NNITSEMGLALLNVADAIRPYPEVVNYLQQVKDDRFLDELIQLEGGQEARDAIEAYLHK
                     YGMRCTGEIDITRTRWKEKPVTLVPMIVSNIKNFEPQAGKRKFEQGLREALEKEQELLE
                     RLKLLPDGEQKAEETKRRIDLIRNFIGYREYPKYGMINRLFVYRQALLKEAEQLVQAGV
                     IHEQEDIYYLTFEELHEVVRANKLDEQIVNKRKDEYKLYEKLTPPRVITSDGEIITGEY
                     DRDHLPAGAIAGLAVSSGVIEGRARVILKMEDADLEEGDILVTPFTDPGWTPLFVSIKG
                     LVTEVGGLMTHGAVIAREYGLPAVVGVDHATKRIKDGQRIRVNGTEGYIELL"
     gene            complement(4295..4897)
                     /locus_tag="SK3146_RS01150"
                     /old_locus_tag="SK3146_00226"
     CDS             complement(4295..4897)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012294250.1"
                     /locus_tag="SK3146_RS01150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SK3146_00226"
                     /product="hypothetical protein"
                     /protein_id="WP_249863334.1"
                     /transl_table=11
                     /translation="MRTDISNWMPLSVKEINEIFSAIPIRWCIAGGWALDLHLRKQTRE
                     HSDVDVIITREEQLTAYRYLKEDWILYRAEDGKLSLWEDGEYLNSTNDVWISKSDESPW
                     AFQLMIIDTEEPYWTYKRDRSIRRPVADIILKTEEGIPYLRPEIQLLHKAGSSKVREKD
                     YRDFQTMLPSLQEQEKVWLKSSLLRQFPQGHDWVRFL"
     gene            complement(5001..6931)
                     /locus_tag="SK3146_RS01155"
                     /old_locus_tag="SK3146_00228"
                     /pseudo=""
     CDS             complement(5001..6931)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03604"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013877478.1"
                     /locus_tag="SK3146_RS01155"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="SK3146_00228"
                     /product="TOMM precursor leader peptide-binding protein"
                     /pseudo=""
                     /sec_met_domain="TIGR03604 (E-value: 4.1e-66, bitscore:
                     222.0, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="YcaO (E-value: 8.1e-51, bitscore: 172.3,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTVVIIGEGPLADTVCKYLSEFPIVRRSAFGEDLPPARLVLVLG
                     GNTSVHLQAEEILQPLGIPWLRCYESDGEGVVGPLVRPGTAGCSQCAENRYSMARNSKE
                     DIGDLLMRLTSSDSLPISVPEVSPFALRHLASIVSAEAAKALQGDKPHSENRIYLVNLI
                     DLNTTIHYILPDASCPVCGQCSEDSKEAAQLTLGSCKKLSRDTYRSRSMSDLQKVLVKD
                     YWDSRTGLFNGREFSFLSTFACAVVNLPLPMANELTGGHSHSFAGSELAAILEGLERYC
                     GIAPRGKRTVVYDSFVNLKDDAIDPLKVGLHAQEHMERPDFPYLRFDPDADISWVWGYS
                     LLQERPILVPELLAYYSLGYGGGFVYETSNGCALGGSREEAILHGILEIVERDSFLMTW
                     YAKLPVPRLDYRSSGDKELVTMIERVRAVTGYEVLLYNTTMENGIPSIWAIAKGERISH
                     "
     gene            complement(6928..8463)
                     /locus_tag="SK3146_RS01160"
                     /old_locus_tag="SK3146_00229"
     CDS             complement(6928..8463)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006677776.1"
                     /locus_tag="SK3146_RS01160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SK3146_00229"
                     /product="hypothetical protein"
                     /protein_id="WP_249863335.1"
                     /transl_table=11
                     /translation="MSSQGEGVLAVGSGTILIELVKAWYESRLSKLSVLAVNMSRAEAE
                     QLKEASEQALTGNPETSPNIILAEAEGVEVNWAAAIRPFSFILYAAEHNDLEELEKLQT
                     ACTAEKKPLLPATNLRGIGMAGPLLHPDGEGGGQSAWRRIHASVFPPGWETYPLYRTTA
                     TLLSNLVVYEYHKRLAGEGEPNRNNHCYILNPLTLEGSWHQVLPHPSLSARKAVVMTTE
                     LERNLESDQEPDVEQWFSWFEGLTSKVSGIFHVWEEGALKQLPLAQCLVQPVDPLSEGF
                     AQLLPAIICSALTHVEARRESGLAGVEAYAARMTPLWIPRLSSSQEDIGIGAGLTFAEA
                     VGRGLVACLTQELERRTLHHERVLTRMEIGRIEDTHCRYYLQALNIQEGEILVASEEPL
                     LGFPVVWVLSDQSWYGAVGLDITLALRRSLQNALMKTESAAVRSVAWSDDKPQRIAVFP
                     AASIGHAARMLSAIKTLKQHHKRLEVFDMRCEPFLKEGLLEVVGVLLGEEESL"
     gene            8738..8851
                     /locus_tag="SK3146_RS01165"
     CDS             8738..8851
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019638725.1"
                     /locus_tag="SK3146_RS01165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YjcZ family sporulation protein"
                     /protein_id="WP_249863336.1"
                     /transl_table=11
                     /translation="MGLGVAGSCSCGGLFTSTGVILVLFILLVIVSRGFLY"
     gene            complement(8900..9583)
                     /locus_tag="SK3146_RS01170"
                     /old_locus_tag="SK3146_00230"
     CDS             complement(8900..9583)
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020621148.1"
                     /locus_tag="SK3146_RS01170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SK3146_00230"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_249863337.1"
                     /transl_table=11
                     /translation="MTANEQSYEVWWNNNEREALMEEGHLKGWLQLIAALKEEDLSEYS
                     VLDFGCNRGGFLRLLHQIKPYKKATGIDLGRQSIDIANERKGSLPIDYRVSGSPEQLEG
                     QFDLAFCLSVIYLIDDLKTHAWKLSKALKPGGVYYVTYTDLTANPSRDFFKEEIEKYSH
                     LNANMHTLDDIASAFSNEGFRVEIKRRVPTDFITVSGKEKYFRNLNDELMSVYENGYIF
                     RFTKM"
     gene            complement(9570..10403)
                     /gene="nikE"
                     /locus_tag="SK3146_RS01175"
                     /old_locus_tag="SK3146_00231"
     CDS             complement(9570..10403)
                     /EC_number="7.2.2.11"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="nikE"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312617.1"
                     /locus_tag="SK3146_RS01175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SK3146_00231"
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="WP_249863338.1"
                     /transl_table=11
                     /translation="MLELRNVSKSYTIGASTLLNKQTLKAADNICLSLEQGMCLGLVGE
                     SGSGKSTLGKMILGLEKPDDGEIWFEGKSLLTMSRRERQSVRRKIQTVFQDCYSSLNPR
                     WPVYESIREPLKNFEKLSAREEKRTVQELLVSVGLSPDDMAKYPHQFSGGQLQRISIAR
                     AIALKPKLIVLDEAVSSLDVLIQHQILKLLDELKEQYLLSYLFISHDIMAVRSISDRLA
                     VMHKGQIVETIHCSDPLSSMKHPASKQLLESVLPSRPRARANIHNNKEWVSNDSQ"
     gene            complement(10447..11247)
                     /locus_tag="SK3146_RS01180"
                     /old_locus_tag="SK3146_00232"
     CDS             complement(10447..11247)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346948.1"
                     /locus_tag="SK3146_RS01180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SK3146_00232"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_249863339.1"
                     /transl_table=11
                     /translation="MDKHIVLSVDHLHISVSTPHGMKKIVNDVSFRLNRGEVLGLVGAS
                     GSGKTMTCLAIMGLLQESAIIEEGSILLEGKNLLQATPAQMRSIRGGRLAVVMQNPMSA
                     FNPTVTIGSHFMETLRAQRSLSRAEARELAETYLGKTGLPRPAEIMKQYPFQLSGGMLQ
                     RAMIAIAMSLQPAVLIADEPTTALDTVHQMRLLEQMRRLCEEQETAMLLVSHDLGVIAY
                     MADEVMVMQEGSIVEKAPVARLFDRPEHPYTRMLLEARPGIGLV"
     misc_feature    complement(11825..11845)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 22.55"
                     /tool="antismash"
     misc_feature    11905..11919
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.77"
                     /tool="antismash"
ORIGIN
        1 taaattttct tcaaaaaagt cgctctgccc gtatttccaa tcttccttca attgagtcca
       61 caagtattct tggatcgatt ggccggccgc tctctcttcc atagtatctg tatatgaaat
      121 cacttcgttc gccagtgaag ttcccattaa tacttgattt tgatacaaaa aaatgccctt
      181 gaattcttta ttggaataca gaaacgttct cagctcggaa ttgaagtcaa aggcctttct
      241 taatagggta ttgtcgtctt ggatatcgct gtcaagcgta aattggaagt cgtccataaa
      301 caactgaaac ttttccttgc tcttataaat ggaatcttcc atcgtttcaa ggttgatcga
      361 cagttcgctt tcattcgaga gttttacgtt atcgataatt aaatgctggg aaacaaagta
      421 aataatcaag gaggaaatga ccatgaatgc gagcaaaagt cctaaaaaca ttccatttaa
      481 tttcattctg aatgacattg taagcgttct ctccttgttg catgattgtg ccggagcctg
      541 tgctcctcct cttgttaacg catacatttt attctataat acttttaaaa taacatgggc
      601 gttatgtaaa ttgaatgtcc aaatcgtcgt aatacttgta taaatgccgt tttctatcat
      661 aattcctgca cttatagaga ggatgcctat gaaatcccaa gtgctctccg tcggcttcgg
      721 ctcgaagcta tccccgtcta aggtgctgcc gcatacgcat gattattggc agcttgagat
      781 tgcaacagaa ggagtcatta catgcaattt gcttggtgag atgcgatctc tggagattgg
      841 cgatatgctc ctggttcccc caggcgtaga gcatggattt gggtacaatg agccgcctgt
      901 cgcctggata tcgatcaagt ttgagcgcga aacaggcgat cttccccatt ggaccgggat
      961 tattcacagc aatatgatta cgaggaagct catctccgcg ttcaagacgg ccattcatgc
     1021 atcgccttac atgcaatacg aaaaagtatt tgtgggcggc tttcttgaaa cgatacttca
     1081 ttacgttcac tccggcgaat tccgggaaat taacgattcg tccgagttgt tagtcaagcg
     1141 aatgatggac agcatatata aacgaaacgg aaaatcgatg acgatctacg aacttgctga
     1201 agaattgtcg tattcacgca gtcacttatc gaagaagttc aaggagaata ccggtgaaag
     1261 cttgaaatcg tttatcgatc atattcggat tcagaagata gaggaattgc ttcgctatag
     1321 agaacaaagc atttccgaaa tcgctgcgga tctcgggttt catgatgtat tttcgttttc
     1381 gaaatttttc aaaaaacata cgggcaaaag cccgcggcaa tataaataag aaggactttg
     1441 tgtcgcttat tggcgaaaca aagtccttcc gatgtttatc cgaggttaca gtagctcgat
     1501 atacccctct gttccgttca cgcggattcg ctgtccgtct ttgatccgtt tggtggcatg
     1561 atctaccccg acaacggccg gcaagccgta ctcgcgcgcg atcactgctc catgggtcat
     1621 cagtccgccg acttcggtga ccagtccttt aatggagacg aacagcggcg tccagccagg
     1681 gtcagtgaag ggggtgacca atatatcccc ttcttccaga tcggcgtcct ccatctttaa
     1741 gatgacgcgt gcccgcccct caatcacccc ggaagaaaca gccagacctg cgatcgctcc
     1801 ggccgggaga tgatcccggt cgtactcacc ggtaatgatt tccccgtccg acgtaatgac
     1861 acgcggggga gtcagttttt catataattt gtactcgtcc tttcgtttgt tgacgatctg
     1921 ctcatccagt ttgtttgcgc gtacgacttc gtgaagctct tcaaacgtga gataatagat
     1981 atcttcttgt tcatgaataa cgccggcttg cacgagttgt tcggcctctt tcagtaaagc
     2041 ttgcctgtac acgaataagc ggttaatcat gccgtatttc ggatattcgc gatagccgat
     2101 aaaattccgg attagatcga tccttcgttt ggtttcctcg gctttttgtt cgccatccgg
     2161 caataacttc aatcgctcca atagctcttg ttctttttcc aacgcttccc ggaggccttg
     2221 ctcaaatttc cgtttcccgg cctgcggctc gaagtttttg atgttactaa caatcatcgg
     2281 gaccagtgta actggttttt ccttccacct ggttctcgta atatcgattt ctccggtaca
     2341 tcgcatcccg tatttatgga gataagcctc gatagcgtct cgggcttcct gtccaccctc
     2401 cagttgaatc agttcgtcca ggaagcgatc gtctttaacc tgctgcaaat aattaacgac
     2461 ttccgggtaa gggcgaatcg catccgcgac attgagcagc gccagcccca tctccgaagt
     2521 aatattgttc ggtacggatt gagaaagcgt atctgctgcg tttttttccc ccagccactt
     2581 gttcatattt tcattgatcc atgctgaagc gtttatggcg gacataaaca cagccgagct
     2641 ttgcgggtca aataagatct tctttaattg ctggatatct tcccgaataa aatcaaataa
     2701 atccgctccc gatttcgttt ggatgttttg ttgcaactct tctatcgaag cttggctacg
     2761 tttaatcaaa tcggaaacga ttgccggacc gttctcgaat ggtgtcggca tatccgtatt
     2821 cattctgttc ggaatcggcg ctgttggatc atttggtaac aattttataa aatcccgctc
     2881 tatgatggtc acgagtgcgc cttttataag cggatcggag cccaaatttt ttaatagagc
     2941 ttcccggccg gcaggtgtag ccagcatcgg tgcaacatca ataaacaacc tcccgccggc
     3001 tttgcgcata ggtgcgggag taattaacag gaaaaaagac aatcctaacg gtttgatggg
     3061 atctgtcatc atttgttgat ggccaacgga taaatagacg tgattgtccc catcattcgc
     3121 ttcagggatc gggtataacg tcgtaatcgg ccgactttga agaatataaa atgcatcttg
     3181 atccaggcac cattcgatat cctgcggctg gccgaagtaa gcttcgatct gtcttccgat
     3241 gtgtgccagc tgtacaattt gccgttcggt aagtgccgga gtcttttgcc ggtcaggatc
     3301 gatctgcttg gtcgctgttc cgccctcttc ccgtccatag atagccaatg ttttggccgc
     3361 tatcctctta tcgacgatag tctcatcctg tactttatac acatcggccg agaccaggcc
     3421 ggagacgagc gcttctccaa ggccgaaacc ggcatcgatc gtcagcagct tgcggttccc
     3481 ggtcatcgga tcggcggtga ataaaatgcc cgaagcctgc gggaacacca ttctttgaac
     3541 gatgaccgat acatacgctt ggctgtggtc aaatccgttc tgcatccggt agatgaccgc
     3601 gcgatccgtg tagagggagg cccagcattt gcgaatatgc tgcaaaatcg cgtccaggcc
     3661 gataatattt aagtaggtgt cttgttggcc ggcaaacgag gcatgcggca aatcttcggc
     3721 cgtcgcgctg gaacgaaccg catacgcatg ttcctcgcca aaccgggaga gatagcgact
     3781 aacggcttcc gtaacatcgg aaggcatttc cgcttcgata atgacttgcc gaatcttccc
     3841 gctaatttca ccgatggact cgcggtcttc tgcttgcagc gtggttagtc tatggagcag
     3901 agcgtgatac gtctcgtttg gttcgatggc ttgttgatat gccgctgttg taacacaaaa
     3961 tccttccggt actcgtattc cctcaatttc agataatttc cctaaatgga atcctttccc
     4021 gccaacgagc gaaagctgcg ttttttccat ttcttgaaaa tcgagaacca acgaatgcat
     4081 ccaacatctc tcctaaccat tcatttgaga aaaacatttg acaagagctt gactggtatg
     4141 ctacaattaa attgtaagat agattaatta tgtccacata aaataaaaag tcgtaaattg
     4201 attatagcac tgtgcctgct catatgcaat ataaagaacc tgattcaatt gaatcaggct
     4261 tttttgattg tattcgagct tcttggatca gcatttacaa aaaacggacc caatcatgac
     4321 cttgcgggaa ctgccggagt aaagaagatt tcagccatac tttttcttgt tcctgaagcg
     4381 acggcagcat cgtttgaaaa tcacggtaat ctttctccct tactttcgaa ctgcctgctt
     4441 tatgcagaag ctggatttcg ggtctcaaat aaggtatgcc ttcttccgtt ttcaaaataa
     4501 tgtccgctac cggccttcga atggatctgt ctctcttata agtccaatag ggttcttcgg
     4561 tatcaatgat catcagctga aaagcccaag gcgactcgtc gctcttacta atccatacgt
     4621 cattcgtcga gttcaaatac tccccgtctt cccacaaact tagcttgccg tcctcggccc
     4681 tgtataatat ccaatcctct tttaaatacc gataagcggt gagctgctct tcacgggtaa
     4741 taataacgtc aacatcgcta tgctccctgg tttgtttgcg cagatgcaag tccaaggccc
     4801 agcccccggc aatacaccaa cgtatcggaa tagccgaaaa aatttcattg atttccttaa
     4861 cactaagcgg catccaattg cttatatccg tcctcaaacc ggttcctcct taacaacctc
     4921 gaattgattg tttgctctgt atgtaataac atacaagaat tatcttcgaa agtataggct
     4981 gataatggag ccttacgggt ctacggaaac ggatgcgggt aaggattcag ctcctccggc
     5041 gacagcttgt gaccggtata tccgagctgc atcggcactt ccaacactct gttcaaacct
     5101 gccaagcggg taagcgtatg tccgaacgtc atcggcagca ttcccggtat cagcactttg
     5161 aagcagtaca gctcgttgcg gagcgtctcg ctcgttgact ggtcgacgac aatgacgtcc
     5221 aactgcaagc cgcgaaatac ctcaagaacc tgcttcagat catccgttaa atcgtcatta
     5281 gacgctgcag ggccgaattc ttcgccgaat gtccgtagag gacgccgttc atccagcaaa
     5341 aatcctagcc gctcctccgc ttccggcaaa ctatacagta tggaatgatc ctccatatat
     5401 tccaccagga atgaatcatg gaacatcgct tcggcatcat ctcggcgttc gttccagctt
     5461 gcctccactc tggtgatcgt tccagccagc tcatacacgg cgctcttggc tgcacggata
     5521 gggtccagat gggctcctgc ggcgcaaaca aggttcaatg actgattcgc tcccccttcg
     5581 ctatagccca gatgcttgga atgccgtttt ccatcgtcgt attgtacaac agcacttcat
     5641 atcccgttac ggctcttacg cgttcgatca tcgtcaccag ttctttgtcg cccgacgaac
     5701 gataatcgag ccgtggaacg ggcagcttgg cgtaccaggt catcaagaac gaatcgcgct
     5761 caacgatttc caaaatgccg tgcaagatgg cttcttcccg gctcccgcct aacgcgcagc
     5821 cgttagaggt ctcatagaca aaaccgcctc catagcccag gctgtaataa gccagcagct
     5881 cgggaaccaa aatcggccgt tcctgcagta acgagtatcc ccaaacccat gaaatatcgg
     5941 catcgggatc gaaccgcaga tagggaaaat ccggccgttc catgtgctcc tgcgcatgga
     6001 gccccacctt caacggatcg atggcatcat ccttcaagtt aacgaagctg tcatacacaa
     6061 ccgttcgttt tcctctgggg gcgatcccgc aatatctctc caatccttcg aggatggctg
     6121 ccagctcgct gcctgcaaac gaatgcgaat gacctcccgt caattcattg gccataggca
     6181 aaggaagatt cacaaccgcg catgcaaaag tggacaggaa gctaaactcc ctgccgttga
     6241 agagcccggt ccggctgtcc caataatcct taaccagaac cttctgcaaa tcgctcatcg
     6301 agcggctgcg gtaggtatcc ctgctgagct ttttgcaaga tccaagcgtt agctgagccg
     6361 cttccttcga atcctccgaa cattggccgc aaacaggaca tgatgcgtcc ggcaaaatgt
     6421 agtgaattgt cgtattcaga tcaatcaggt taaccagata gatgcggttt tccgaatgcg
     6481 gtttatctcc ttgaagcgct ttagccgctt ctgcagaaac tatagacgcc agatgccgca
     6541 atgcgaaagg agatacctcc ggaaccgata tagggagaga atcggaggag gtgagtctca
     6601 tgagcaggtc gcctatatcc tccttgctgt tccttgccat ggagtacctg ttttccgcgc
     6661 attgcgaaca gcccgccgtc ccgggacgga ctagcggccc caccactcct tctccatcgg
     6721 attcataaca gcgcagccaa gggatgccga gcggctgcag tatctcttct gcctggagat
     6781 gaacggaggt gttcccgccc agcaccaata ccagcctcgc aggcggcaaa tcctcgccga
     6841 aagcggatct gcgcactatc gggaattccg acaaatactt gcacaccgta tccgccaacg
     6901 gcccctctcc gataataacg acggtattca tagggattcc tcctcaccga gcagtacgcc
     6961 aacgacttca agcagccctt ctttcagaaa aggctcgcac cgcatgtcga acacctccag
     7021 ccgtttatga tgctgcttca aggtctttat cgctgaaagc attctagcgg cgtgaccgat
     7081 ggaggctgca ggaaaaacgg cgatgcgctg cggcttatca tcgctccaag caacggaacg
     7141 aaccgcggcg ctttccgttt tcatcaatgc attttgcagc gatcggcgaa gcgccaaggt
     7201 tatgtcaagg cctactgcgc cataccagga ttgatccgag aggacccata cgaccggaaa
     7261 cccgagcaac ggctcttcgg atgctacgag aatctcgccc tcctgaatat tcagagcttg
     7321 caaataatac cggcagtgag tatcttcgat tcggccaatt tccatgcggg tgaggaccct
     7381 ctcatgatga agggtccgtc tttccagctc ttgggtcaaa caagcgacca gcccgcgccc
     7441 gaccgcttcg gcgaacgtga gtcctgctcc gatgccgatg tcctcctggc ttgaggatag
     7501 ccgaggtatc catagcgggg tcatgcgagc ggcataggct tccactcctg caagcccgga
     7561 ctcccgccgg gcctctacat gcgttaaagc gctgcagatg atggccggca aaagctgcgc
     7621 aaagccttcg gatagcgggt caacgggctg cacgagacat tgcgccagcg gaagctgctt
     7681 caacgcacct tcctcccaaa catggaaaat acccgacacc ttggaggtca gcccttcaaa
     7741 ccaagaaaac cattgctcca catccggttc ctgatccgat tcgagatttc gctccagctc
     7801 cgtcgtcatg acgaccgcct tgcgagccga taaggaagga tgcgggagaa cctgatgcca
     7861 gcttccctcc agcgtgagcg gattcaagat ataacaatga ttattgcgat tcggctcgcc
     7921 ctctccggca agccgtttat ggtattcata tacgacaaga ttggataaga gggttgcggt
     7981 cgtcctatat aaaggatacg tttcccatcc aggagggaat acggacgcgt gaatgcgccg
     8041 ccatgcactc tgcccgcccc cctctccgtc cggatgaagc agcggcccgg ccatgcctat
     8101 tcctcgcaga ttcgttgcag gaaggagcgg tttcttttct gccgtgcagg cggtctgaag
     8161 cttttcaagc tcctccaggt cgttgtgctc agcagcatac aaaataaacg aaaacggcct
     8221 gatcgccgcg gcccaattca cttcgactcc ctccgcttcg gctagaataa tattcggaga
     8281 tgtttccgga ttgcccgtaa gcgcctgttc cgaggcttcc ttaagctgct ccgcttcggc
     8341 tcgcgacatg ttgactgcca acacggatag cttcgataaa cgcgattcat accatgcttt
     8401 aaccagctcg atcaagatgg ttcctgaacc gactgccagt accccttcac cttgagaaga
     8461 cattagtcta cctccatcca tgacattaca cggttcgaga acccggtcat accgttatcc
     8521 gcaaacccaa aatctactgt aaaaatatgt cggaatcctt atttttatta cgtaaccaaa
     8581 gcaaggcagc gttaaattct tcggcagcaa gtccgtgaaa ggttgggggt gcaattaaac
     8641 ttaacgctct aatatttaaa caattttccg ttttgcccag tccatgagcg gcacaaacac
     8701 atacaatacc ttagaactcg ggagggagga agtcgcaatg ggattaggag tagcaggtag
     8761 ctgcagttgt ggaggtttgt ttacttctac gggtgtgatt ctcgtacttt tcattctgct
     8821 cgtcattgtt tctcgcggat ttctctacta agtaggtaag cataaacgcc cgactattca
     8881 aatagtccgg gcgttttttt tacattttcg taaatcggaa tatataaccg ttctcataga
     8941 cggacatcaa ttcgtcgttc aagttcctga aatatttctc cttgccggag actgtaataa
     9001 agtcggtcgg cactcttcgc ttgatttcca cccgaaaccc ctcgttacta aaagcggagg
     9061 caatatcgtc gagggtatgc atattagcgt tcaaatggct gtacttttca atctcttctt
     9121 taaaaaaatc gcggctgggg ttcgccgtta aatccgtgta ggtaacataa taaacgccgc
     9181 cgggctttag cgccttgctc aacttccagg catgcgtctt taaatcatcg atcaaataaa
     9241 tgacggacag acaaaaagca agatcgaatt ggccttcgag ctgttcagga gatccagata
     9301 ccctataatc gataggtaag ctgccctttc tttcattggc aatgtcaatg gactgccgtc
     9361 ccaaatcgat gcctgtcgct ttcttgtacg gcttgatttg atgcagcagc cttaaaaagc
     9421 cgccccgatt acaaccgaag tccagaacgg aatattccga taaatcttct tcctttaagg
     9481 ctgcgatcag ctgaagccag cccttcagat gcccttcttc cattagagct tcgcgttcgt
     9541 tgttgttcca ccatacctca taactttgtt cattggctgt cattgcttac ccactcctta
     9601 ttattatgaa tatttgcccg agctctcggg cggctaggga gaacggattc caaaagctgc
     9661 ttggatgcag gatgcttcat tgaagacagc gggtcggaac aatgtattgt ttcgacaatt
     9721 tgtcctttgt gcatgaccgc caatcgatcc gagattgaac gaacggccat tatatcgtgg
     9781 gagataaaca agtaggacaa caaatactgc tctttcaact catccagaag cttcagaatt
     9841 tgatgctgga tgagcacgtc aagactgctg acggcttcat ccaggactat tagcttgggc
     9901 ttgagcgcga tggcgcgggc aatacttatt cgttgcaatt gcccgccgct aaattgatga
     9961 ggatacttag ccatatcatc cgggcttaag cctaccgata ccagtaattc ttgaacggtt
    10021 ctcttttcct cgcgcgcgct cagtttctcg aaatttttta gaggttctct tatggattca
    10081 tagacaggcc atctaggatt caatgagctg tagcaatctt gaaaaaccgt ttgaattttg
    10141 cggcgaacgc tctggcgttc tctcctgctc atggttaaca agcttttgcc ttcaaaccaa
    10201 atttcgccgt catccggctt ctccagaccc aagatcatct tacccagcgt actcttgccg
    10261 gagccgctct cgccgaccag ccccagacac atgccttgct ccaaggaaag gcaaatgttg
    10321 tccgccgctt tcaaggtttg cttattcaac aaggttgagg cacctatagt atagctttta
    10381 cttacattcc ttaattcaag catactccca cccctaagaa agcttcctgt tcgcaacacg
    10441 agcattttaa acgagaccga tacccggtct tgcttccagc agcatacggg tatacggatg
    10501 ctctggacga tcgaacaacc gggcgaccgg ggctttttct acaatgctcc cctcttgcat
    10561 gaccatcacc tcgtcagcca tataggcaat aacgccaaga tcatgggaaa cgaggagcat
    10621 agccgtttcc tgctcctcac acagacggcg catttgctcc agcaaccgca tctgatgaac
    10681 cgtatccaaa gccgtggtag gctcatcggc gatcaataca gccggctgca acgacatcgc
    10741 gatggcgatc atcgcccgct gaagcatccc tccgctcaat tgaaacggat actgcttcat
    10801 gatttcagcc gggcgaggca gaccggtttt accgagatac gtctctgcca gctcgcgagc
    10861 ttccgcccgc gacaatgaac gttgggctcg aagcgtctcc ataaaatgac tgcctatcgt
    10921 caccgtaggg ttaaaggcgc tcatcggatt ttgcataacg accgcgagac gcccgcctcg
    10981 tatgcttctc atttgagctg gggtcgcctg cagcagattt ttgccttcca gaagtatcga
    11041 tccctcttct atgatggcgg actcctggag caggcccatg atcgccaggc aagtcatcgt
    11101 tttcccgctc ccgctggccc ccacaagtcc tagtacctct ccgcggttta atcggaagct
    11161 gacatcgttc actattttct tcattccatg aggcgttgac acgctaatat gcagatgatc
    11221 tacgcttaaa acgatgtgct tgtccatgga cactatctca tcctttcttg acgtggatct
    11281 aacgcatccc tcaaagcgtc tccgataaaa ttaaaggcca tgacgaccag cataatacat
    11341 aagcccggat acagcatcag catcggattg cttttcagga attgcttgct gtcattcagc
    11401 atggcccccc actccggcat aggaggctga atgcccagtc ccaaaaagga gaggccggaa
    11461 atgcttaaaa tcatactccc caaatttaaa gtggccaata cgattgtagg aggccatata
    11521 ttaggaagaa tgtggaacca aataatttta taaccgggag ttccggcgat tttagcggct
    11581 aaaataaaat tttgctgctt ggccgtcatg accataccgc gaatccagcg cgcataccac
    11641 acccagtaag acatgaccat cgcaaagatg acgttgacca gtccgggtcc gagaattccg
    11701 agcatggcaa gagatagaat gagactggga aatgccatta acaaatcgca aaaacgcata
    11761 aagatgcgat cgataacgcc gccgctatat cccgataacg ttccgatagc tacactgatg
    11821 ataataatca acaccatcac gataaaagaa atccctagtg aaattcttgt cccgtagatc
    11881 aatcgggata agatgtctct gcccagatga tccgttccaa gccaataggc a
//
