LOCUS       NZ_CP090423            10876 bp    DNA     circular CON 08-MAY-2024
DEFINITION  Stenotrophomonas maltophilia strain GYH chromosome, complete genome.
ACCESSION   NZ_CP090423
VERSION     NZ_CP090423.1
KEYWORDS    RefSeq.
SOURCE      Stenotrophomonas maltophilia
  ORGANISM  Stenotrophomonas maltophilia
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Stenotrophomonas; Stenotrophomonas maltophilia
            group.
REFERENCE   1  (bases 1 to 4791742)
  AUTHORS   Cheng,Z., Zhao,P., Chen,J., Zhao,J., Yu,J., Zhang,S. and Guan,Y.
  TITLE     Aerobic biodegradation of trichloromethane by Stenotrophomonas
            maltophilia GYH: Biodegradation characterization, genome functional
            analysis and metabolic pathway determination
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4791742)
  AUTHORS   Cheng,Z., Zhao,P., Chen,J., Zhao,J., Yu,J., Zhang,S. and Guan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-DEC-2021) college of environment, Zhejiang university
            of technology, 18 Chaowang RD, Xiacheng District, HangZhou, Zhejiang
            313200, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. v1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 27.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023518235.1-RS_2024_05_06
            Annotation Date                   :: 05/06/2024 14:53:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,547
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,428
            CDSs (with protein)               :: 4,428
            Genes (RNA)                       :: 92
            rRNAs                             :: 5, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 8 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 0 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:15:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1904423
            Orig. end                         :: 1915299
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP090423.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zhejiang university of
            technology.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10876
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10876
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10876
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5876](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5876
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(210..224)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.74"
                     /tool="antismash"
     misc_feature    461..474
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 20.06"
                     /tool="antismash"
     gene            942..1910
                     /gene="pstC"
                     /locus_tag="LZ605_RS09055"
                     /old_locus_tag="LZ605_09055"
     CDS             942..1910
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene="pstC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008572163.1"
                     /locus_tag="LZ605_RS09055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZ605_09055"
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="WP_249844551.1"
                     /transl_table=11
                     /translation="MNAIAQPVAAPSTRDARDARNDKLFRWVLVGTVVFVLIALACAAL
                     SMLWGGRHALQMQGLSFFFSADWNPVENKFGALAPIYGTLVTALIAMVIAVPVSFGIAF
                     FLTEVAPRWLRGPVGTAIELLAGIPSIIYGMWGLFVLVPVMTEYVTPFLNETLGEWPII
                     GPMFQGPPLGIGMLTAGFVLAIMVIPFISSVMREVFLTVPTRLKESAYALGSTKWEVSW
                     DIVLPYTRSAVIGGIFLGLGRALGETMAVAFVIGNSVRLSPSLLEPGTTIAALIANDFG
                     EATETYRSALLLLGFVLFIVTFVVLAIARLMLMRLSRKEGN"
     gene            1910..2773
                     /gene="pstA"
                     /locus_tag="LZ605_RS09060"
                     /old_locus_tag="LZ605_09060"
     CDS             1910..2773
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene="pstA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412770.1"
                     /locus_tag="LZ605_RS09060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZ605_09060"
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="WP_057498349.1"
                     /transl_table=11
                     /translation="MSTTADSLYLRRRIGNVVAIAASCATALFGLFFLGWILFTLASKG
                     LAGINWDLFTKMTPPPMQEGGLANAFFGSAVMCALAIGIGTPLGVLAGTWLAEYGNARK
                     AGTVVRFVNDILLSAPSIVLGLFVYTLYVMQTGGNFSAFAGALSLAFIVLPVVVRTTDE
                     MLRLVPSQMREAALSLGIPQWKVIVQVLYRSASAGIITGILLALARISGETAPLLFTAF
                     GNQYWNNNIFQPMASVPVVMNQFAGSPYESWQVLAWAGALVLTVFVLLVSLAARGILLR
                     NRISHD"
     gene            2793..3623
                     /gene="pstB"
                     /locus_tag="LZ605_RS09065"
                     /old_locus_tag="LZ605_09065"
     CDS             2793..3623
                     /EC_number="7.3.2.1"
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene="pstB"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237787.1"
                     /locus_tag="LZ605_RS09065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZ605_09065"
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="WP_057498350.1"
                     /transl_table=11
                     /translation="MNDLSNAVPMQRIAVPSSHESLHTPAPVKLAARGLDFYYDKFHAL
                     KGINLEIPEKRVTALIGPSGCGKSTLLRIFNRIYALYPKLEARGEVLLDGENILSPKYP
                     MNRLRSKVGMVFQKPVPFPMTIFENVAYGIRHHEKLSKADMADRVEQALRQGALWDEVK
                     DKLGQSALGLSGGQQQRLCIARAVALRPSVLLLDEPTSALDPISTSRIEQLVEELKNEY
                     TIVIVTHNMQQAARVSDYTAFMYLGDLIEHDRTEVIFSQPSQRQTEDYITGRFG"
     misc_feature    complement(3689..3704)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.95"
                     /tool="antismash"
     gene            3700..4407
                     /gene="phoU"
                     /locus_tag="LZ605_RS09070"
                     /old_locus_tag="LZ605_09070"
     CDS             3700..4407
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /function="Phosphate uptake regulator"
                     /gene="phoU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467248.1"
                     /locus_tag="LZ605_RS09070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZ605_09070"
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="WP_006428132.1"
                     /transl_table=11
                     /translation="MNLPNDHIVKSYDEEQQRLVAEIVRMGEMAVAQLEAAMDVIEKRD
                     ENAAHRIIANDEAIDALEQQISHDVMRLALRGPMARDLREILAGLRIPADIERIGDYAA
                     NVAKRSIALGKVPPLPQIQGLRALGRLAAQQVRRAIAAYRDNDADAALALREDDARLDA
                     QYTALFRELLTYMMEDPRNITPCTHLLFMAKNLERVGDHATNIAENVWFLVHGEQPLPP
                     REKRDETSSTGQI"
     misc_feature    complement(3713..3728)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.64"
                     /tool="antismash"
     misc_feature    4538..4553
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.19"
                     /tool="antismash"
     gene            4667..5014
                     /locus_tag="LZ605_RS09075"
                     /old_locus_tag="LZ605_09075"
     CDS             4667..5014
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646537.1"
                     /locus_tag="LZ605_RS09075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZ605_09075"
                     /product="hypothetical protein"
                     /protein_id="WP_249844552.1"
                     /transl_table=11
                     /translation="MSSSNKTLSLLTATALAAGLGMTASASALSMSDLAQGYLVAGQAA
                     KASTDAKAADTKMAADASKHAEGKCGADGKTAEGKCGADKGKAAGAAAAKTGDKKSAEG
                     KCGEGKCGGKH"
     gene            5001..5876
                     /locus_tag="LZ605_RS09080"
                     /old_locus_tag="LZ605_09080"
     CDS             5001..5876
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014036545.1"
                     /locus_tag="LZ605_RS09080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZ605_09080"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_249844553.1"
                     /sec_met_domain="DUF692 (E-value: 3.8e-115, bitscore:
                     381.7, seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASTDVRASVVHPRSPLRAAAAGLGLRRALLQDLRDAPAGDFDFL
                     ECAPENWIHVGGPAGDALAELAQRHPLSCHGLSLSLGGSAALDTQLLEQVGQFLEKHRV
                     PLYSEHLSYCSDEGHLYDLLPIPFTDEAVRHTAARIARVQELLGRRIAVENVSYYLAPE
                     PAMDELAFTTAVLAEADCDLLLDVNNVYVNACNHGYDADAFIAGLPADRIVCLHVAGHL
                     DEAPDLKIDTHGSAVIDPVWALLARTYARIGPRPTLLERDFNFPPYAELQGELQTIRRL
                     QAMHAGAAHG"
     gene            5869..6624
                     /locus_tag="LZ605_RS09085"
                     /old_locus_tag="LZ605_09085"
     CDS             5869..6624
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412767.1"
                     /locus_tag="LZ605_RS09085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZ605_09085"
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="WP_249844554.1"
                     /transl_table=11
                     /translation="MAEAPATLRAQQHAFTAHLRDPQAVSAPAGLDPRRVAVYQRLLFN
                     NLLGLLSTGFPVCMRLLGEPAWNTLVRHYFATHRCQTPLFTELAAEFVQWLQAQPTLPH
                     PALAELAHYEWVETALYQLSAEPLPAPGDVDPLRVPLQRSPLAWPLLYGWPVHRLGAED
                     APSALPSEPTGLLVRRDPDGEVRFAALSPLAVHLLSRIGEQPGLDGHRYLQQLATDHGL
                     GADALAEPGAALLQQFLRAGVIGPLAPPR"
     gene            6650..7144
                     /locus_tag="LZ605_RS09090"
                     /old_locus_tag="LZ605_09090"
     CDS             6650..7144
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019659083.1"
                     /locus_tag="LZ605_RS09090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZ605_09090"
                     /product="DoxX family protein"
                     /protein_id="WP_249844555.1"
                     /transl_table=11
                     /translation="MNLPILAATRSQLDRLAPWLAPLGLRLLLAWEYFESGREKLQGQN
                     WFADLQDAFPFPFDQVPAALNWQLATWFELVGAACLLFGFGTRLAAASLLVLTVVATYA
                     VHWPMQWDTLGELAMGYAISDQGFGNFKLPALFMAMLLPLLFTGAGKLSVDAVLARPAV
                     R"
     gene            7336..7920
                     /gene="rnt"
                     /locus_tag="LZ605_RS09095"
                     /old_locus_tag="LZ605_09095"
     CDS             7336..7920
                     /GO_function="GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /GO_process="GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="rnt"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408782.1"
                     /locus_tag="LZ605_RS09095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LZ605_09095"
                     /product="ribonuclease T"
                     /protein_id="WP_107230365.1"
                     /transl_table=11
                     /translation="MSQRFRGFLPVVVDVETGGFDSQRNALLEIAAVPIEMDENGLLYP
                     GQTASAHVVPAEGLEIDPKSLEVTGIILDHPFRLAKEEKAALDHIFTPVRAAMKKYGCQ
                     RAILVGHNAHFDLGFVNAAVARTGHKRNPFHPFSVFDTVTLAGIAYGQTVLARAAHAAG
                     LGWDANEAHSAVYDTEQTARLFCTIANAWPR"
     misc_feature    complement(9324..9338)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.64"
                     /tool="antismash"
ORIGIN
        1 gatcaactac cagtcgatcg gttccggcgg cggcatcgcc cagatcaagg ccggcaccgt
       61 cgatttcggt tcctccgaca agccgctgag cagcgaagag ctggcccagg ccggcctggc
      121 gcagttcccg tccgccatcg gcggcgtggt gccagtggtc aacatcgaag gcctggaagc
      181 cggcaaactg cgcctgagcg gcgccctgct ggccgacatc ttcctgggca aggtcaagac
      241 ctggaacgat ccggccatcg tcgccgccaa cccgggcgtg aagctgccgg acggcaagat
      301 caccctggtc caccgttcgg acggttcggg caccaccttc aacttctcca actacctgtc
      361 caaggtcagc ccggagtgga agagcaaggt tggcgaaggc acctcggtgc agtggccgga
      421 tggcgtcggt ggcaagggca atgaaggcgt cgcttcgtac gtgaagcaga tcaagggctc
      481 gatcggctac gtcgaactgg cctatgcgct gcagaacggc atgccgtaca ccgccatgca
      541 gaacgcggcc ggcaactggg tcgcgccgag cgcggaaacc ttcgccgccg cagcagccag
      601 cgccgactgg gccagcgcca aggacttcaa cctggtcatc accaacgccc cgggcgagca
      661 ggcgtggccg atcaccgcca ccaacttcat gctgatgcag aagcagccga aggacgccaa
      721 gcgcaaccag gacaccctgg ccttcttcaa gtgggccttc gagaacggcc aggcccaggc
      781 caacgagctg cactacgtgc cgctgccggc cgagctggtc aagcagatcg aggcctactg
      841 ggcgaccgac ctgaagtgat ggacacgacg gtgcccgccc cgcgcgggca ccgctgcttc
      901 cacgccgtac cgccccgccc cttcctggcc ctgccgtccc catgaatgcc atcgcccagc
      961 ctgtagccgc cccgtccacg cgtgatgcgc gtgatgcccg caacgacaaa ctgttccgat
     1021 gggtgctggt cggcaccgtc gtcttcgtcc tgatcgccct ggcctgcgcc gcgctgtcga
     1081 tgctgtgggg cggccgccat gccctgcaga tgcagggcct gagcttcttc ttctcggccg
     1141 actggaatcc ggtcgaaaac aagttcggcg ccctcgctcc gatctacggc accctggtca
     1201 ccgcactgat cgcgatggtc atcgccgtgc cggtcagctt cggcatcgcc ttcttcctca
     1261 ccgaagtcgc tccgcgctgg ctgcgcggcc cggtcggcac cgccatcgaa ctgctggccg
     1321 gcatcccctc gatcatctac ggcatgtggg gcctgttcgt gctggtgccg gtgatgaccg
     1381 agtacgtcac cccgttcctc aacgaaaccc tgggcgaatg gccgatcatc ggcccgatgt
     1441 tccagggccc gccgctgggc atcggcatgc tcaccgccgg tttcgtgctg gccatcatgg
     1501 tcatcccgtt catctcctcg gtgatgcgcg aagtgttcct gaccgtgccg acccgcctta
     1561 aggaatcggc ctatgcgctg ggttccacca agtgggaagt gagctgggac atcgtgctgc
     1621 cctacacccg ctcggcggtg atcggcggca tcttcctcgg cctcggccgc gccctgggcg
     1681 agacgatggc ggtggccttc gtgatcggca acagcgtgcg cctgtcgccg tcgctgctgg
     1741 aaccgggcac caccatcgcc gcgctgatcg ccaacgactt cggcgaagcc actgaaacct
     1801 atcgttcggc cctgctgctg ctcggcttcg tcctgttcat cgtcaccttc gtggtgctgg
     1861 ccatcgcccg cctgatgctg atgcgcctgt cccgcaagga gggcaactga tgtccactac
     1921 cgctgattca ctgtacctgc gccgccgcat cggcaatgtc gttgccatcg cggcgtcctg
     1981 cgccaccgcc ctgttcggcc tgttcttcct gggctggatc ctgtttaccc tggcctcgaa
     2041 gggcctggcc ggcatcaact gggatctgtt caccaagatg acaccgccgc cgatgcagga
     2101 aggcggcctg gccaacgcct tcttcggcag tgcggtgatg tgtgcactgg ccatcggcat
     2161 cggcaccccg ctgggcgtgc tggccggcac ctggctggcc gagtacggca acgcccgcaa
     2221 ggccggcacc gtggtccgct tcgtcaacga catcctgctg tcggccccgt ccatcgtgct
     2281 cggcctgttc gtctacacgc tgtacgtgat gcagaccggc ggcaacttct cggccttcgc
     2341 cggtgcgctg tcgctggcct tcatcgtgct gccggtggtg gtgcgcacca ccgacgagat
     2401 gctgcgcctg gtgccctcgc agatgcgcga agcggcgctg tcgctgggca tcccgcagtg
     2461 gaaggtgatc gtgcaggtgc tgtaccgcag cgcgtcggcc ggcatcatca ccggcatcct
     2521 gctggcgctg gcccgcatct ccggcgagac cgcgccgctg ctgttcaccg ccttcggcaa
     2581 ccagtactgg aacaacaaca tcttccagcc gatggcctcg gtaccggtgg tgatgaacca
     2641 gttcgccggc agcccgtatg aatcctggca ggtgctggcc tgggccggtg ccctggtgct
     2701 caccgtcttc gtgttgctgg tcagccttgc cgcccgcggc atcctgctgc gcaaccgtat
     2761 ctcccatgac tgacctttcc gtggataacg ccatgaacga cctttccaac gccgtgccga
     2821 tgcagcgcat cgccgtgccg tcctcgcacg agagcctgca cacgccggct ccggtcaagc
     2881 ttgccgcgcg cggactggac ttctactacg acaagttcca tgccttgaag ggcatcaacc
     2941 tggagattcc ggaaaagcgc gtgaccgcgc tgatcggtcc ctccggttgc ggcaagtcga
     3001 ccctgctgcg catcttcaac cgcatctacg cgctgtaccc gaagctggaa gcgcgcggtg
     3061 aggtgctgct ggacggcgag aacatcctgt cgccgaagta cccgatgaac cgcctgcgca
     3121 gcaaggtcgg catggtgttc cagaagccgg tgccgttccc gatgaccatc ttcgagaacg
     3181 tggcctacgg catccgccac cacgagaagc tgagcaaggc cgacatggcc gaccgcgtcg
     3241 agcaggcgct gcgccagggc gcgctgtggg acgaagtgaa ggacaagctg gggcagagcg
     3301 cactgggcct gtctggcggc cagcagcagc gcctgtgcat cgcccgtgcc gtggccctgc
     3361 gcccgtcggt gctactgctg gacgagccga cctcggcgct ggacccgatc tccaccagcc
     3421 gcatcgagca gctggtcgaa gagctgaaga acgagtacac catcgtcatc gtcacccaca
     3481 acatgcagca ggccgcgcgc gtgtccgact acaccgcctt catgtacctg ggtgacctga
     3541 tcgagcacga ccgtaccgaa gtgatcttct cgcagccgtc tcagcggcag accgaggact
     3601 acatcaccgg tcgcttcggc tgaaacggca gtcgagcgtg gctcgactct acagacaacg
     3661 gcagtcgagc atggctcgac tctacagagt ggaacaacca tgaatcttcc caacgaccac
     3721 atcgtcaaga gctacgacga agaacagcag cgcctggtgg ccgaaatcgt ccgcatgggc
     3781 gagatggccg tcgcccagct cgaagcggcg atggacgtga tcgagaagcg cgacgagaac
     3841 gctgcccatc gcatcattgc caatgatgaa gcgatcgacg cgctggaaca gcagatcagc
     3901 cacgacgtga tgcgcctggc gctgcgtggg ccgatggcgc gcgacctgcg cgagatcctc
     3961 gccggcctgc gcatcccggc ggacatcgag cgcatcggcg actacgcggc caacgtggcc
     4021 aagcgctcga tcgcgctggg caaggtgccg ccgctgccgc agatccaggg cctgcgcgcg
     4081 ctgggccgcc ttgccgcgca gcaggtgcgc cgcgccatcg ccgcctaccg cgacaacgac
     4141 gccgacgccg cacttgcgct gcgcgaagac gacgcgcgcc tggacgcgca gtacaccgcg
     4201 ctgttccgcg agctgctgac ctacatgatg gaagacccgc gcaacatcac cccgtgcacc
     4261 cacctgctgt tcatggccaa gaacctggag cgcgtgggcg accacgccac caacattgcc
     4321 gagaacgtgt ggttcctggt gcacggcgag cagccgctgc cgccgcgtga aaagcgcgat
     4381 gagacctcca gcaccggcca gatctgacgc tgcactgcaa caccggagtt gactccaagg
     4441 tggccgctgt cctggtgaca gcggccttct tttttggggt attccccccg gcatcgtggg
     4501 cgccgggctt gcccggtacg gaccttccgg atgcttctgt aacaaacctc acgaaaattt
     4561 gagttcttgg cgaggattcg ttgagtagga tcgctgcacc atcgcttcca ccggcattcc
     4621 tgccggccac ccgggcatct gcccacacca cccaggagca cgaccgatga gcagttccaa
     4681 caagaccctg tccctgctga cggctaccgc cctggccgcc ggcctgggca tgaccgccag
     4741 tgcctcggcc ctgagcatga gcgacctggc ccagggctac ctggtggccg gtcaggcggc
     4801 caaggccagc accgacgcca aggccgccga caccaagatg gccgctgacg ccagcaagca
     4861 cgccgaaggc aaatgtggcg ccgacggcaa gaccgccgaa ggcaagtgcg gcgccgacaa
     4921 aggcaaggct gccggtgcgg ccgccgccaa gaccggcgac aagaagtcag ccgaaggcaa
     4981 gtgcggcgaa ggcaagtgcg gtggcaagca ctgacgtccg cgccagcgtc gtccacccac
     5041 ggtcgccgct tcgcgcggcg gccgcggggt tgggcctgcg ccgggcgctg ctgcaggacc
     5101 tgcgcgatgc accggccggc gacttcgatt tcctcgaatg tgcgccggag aactggatcc
     5161 acgttggcgg cccggccggt gatgcgctgg ccgaactggc acagcgccat ccactgagct
     5221 gccatgggct gtcgctatca ctgggtggca gcgcagcgct ggatacgcag ctgctggaac
     5281 aggtcggcca gttcctggaa aaacaccgcg tgccgctgta cagcgaacac ctgagctact
     5341 gcagcgacga aggtcatctg tacgacctgc tgccgattcc gttcaccgac gaagccgtgc
     5401 gccacaccgc cgcgcgcatt gcccgcgtac aggaactgct cggtcgccgc atcgccgtgg
     5461 agaacgtgtc gtactacctg gcgccggaac cggcgatgga tgagctggca ttcaccacgg
     5521 ccgtgctcgc cgaagccgac tgcgacctgc tgctggacgt caacaacgtt tacgtcaacg
     5581 cctgcaacca cggctacgat gccgatgcct tcatcgccgg cctgcccgcc gaccgcatcg
     5641 tctgcctgca cgttgccggg cacctggacg aagcgccgga cctgaagatc gatacccatg
     5701 gcagcgcggt gatcgacccg gtctgggcac tgctggcgcg cacctatgcg cgcatcggtc
     5761 cgcgccccac cctgctcgaa cgcgatttca atttcccgcc ctacgccgag ctgcagggcg
     5821 agctgcagac catccgccgc ctgcaggcca tgcacgcggg agcggcacat ggctgaggct
     5881 cccgccacgc tgcgcgcaca gcagcacgcc ttcaccgcgc atctgcgcga cccgcaggcg
     5941 gtgtccgcac cggccgggct ggacccgcgc cgggtggcgg tgtatcagcg cctgctgttc
     6001 aacaacctgc tcggcctgtt gagcactgga ttcccggtct gcatgcgcct gctcggcgaa
     6061 ccggcctgga acacgctggt gcgccactac ttcgccaccc accgctgcca gacaccgttg
     6121 ttcaccgaac tggccgccga gttcgtgcag tggctgcagg cgcagcccac gctgccgcat
     6181 ccggcgctgg ccgaactggc ccactacgag tgggtggaaa ccgccctgta ccagttgtcc
     6241 gccgaacccc tgcccgcgcc tggcgatgtc gatccgttgc gggtgccgct gcagcgctcg
     6301 ccgctggcct ggccactgct gtacgggtgg ccggtccatc gcctgggtgc cgaggatgcg
     6361 ccaagcgcgt tgccgtccga accgactggc ctgctggtgc gccgcgaccc cgacggtgag
     6421 gtgcggttcg ctgcgctcag cccactggcg gtgcacctgc tgtcccggat cggcgaacag
     6481 cctggcctgg atggccatcg ctacctccag cagctcgcca ccgaccatgg cctgggcgcc
     6541 gatgcgctgg ccgaacccgg tgccgcgctg ctgcagcagt tcctgcgcgc cggcgtgatc
     6601 ggcccgcttg ctccaccgcg ctgacacccc ttccacggag attccgccga tgaacctgcc
     6661 gatcctggct gccacgcgca gccagctgga ccgtctcgcc ccctggttgg ccccgctcgg
     6721 cctgcgcctg ctgctggcct gggaatattt cgaatccggc cgcgagaagc tgcagggcca
     6781 gaactggttt gccgacctgc aggacgcctt cccgttcccc ttcgatcagg tacccgcggc
     6841 actgaactgg cagctggcga cctggttcga gctggtcggc gcagcctgcc tgctgttcgg
     6901 tttcggcacg cgtttggcgg cggccagcct gctggtgctg accgtggtgg ccacctatgc
     6961 cgtgcactgg ccgatgcaat gggacacgct gggcgaactg gcgatgggct atgcgatcag
     7021 cgaccagggc ttcggcaact tcaagctgcc cgcgctgttc atggcgatgc tgctgccgct
     7081 gctgttcacc ggcgccggca agctgagcgt ggacgccgtg ctggcacggc ctgcggttcg
     7141 atagatgctg accttggtcg gcgcgggttg tgccaaccaa ggttggcacc taccgggctc
     7201 gccggccttg gtcggcgtgg gcgcttgagc atggccgggc actcggggcc gctctgctag
     7261 aattcaccca tgactgaccc cacccccgca cctgccgccg cccccgattc cgcaacatcc
     7321 ccggcgcagc gcgcgatgtc ccagcgcttc cgcggcttcc tgccggtggt ggtggacgtg
     7381 gaaaccggcg gcttcgacag ccagcgcaac gccctgctgg aaatcgccgc ggtgccgatc
     7441 gagatggacg agaacggcct gctctacccc ggccagaccg ccagtgccca tgtggtgccg
     7501 gccgagggcc tggagatcga cccgaaatcg ctggaggtga ccggcatcat cctcgaccat
     7561 ccgttccggc tggccaagga agagaaggcg gcgctggacc acatcttcac cccggtgcgt
     7621 gcggcgatga agaagtacgg ctgccagcgc gcgatcctgg tcggccacaa cgcccatttc
     7681 gacctgggct tcgtcaatgc ggcggtggca cgcaccggcc acaagcgcaa tccgttccat
     7741 ccgttcagcg tgttcgacac ggtcaccctg gccggcatcg cgtacgggca gaccgtgctg
     7801 gcgcgcgcgg cccatgccgc cgggctgggc tgggacgcca acgaggcaca cagcgcggtg
     7861 tatgacaccg agcagaccgc gcggctgttc tgcaccatcg ccaacgcctg gccgcggtaa
     7921 accgcgacgg cgcatcccat caggcagtgc ggaccaacga tccgcccccc accgggcggt
     7981 agatccacgc catgcgtgga tgaattcacc cgatgcaggt gcgatcccgc ccgtcgctct
     8041 tggcgcggta cagcgcacgg tcggcgcgct ggtacagcaa ggccggcgtg cgatcgtcgg
     8101 catccagttc caccaggccg gcactgaagg tcacccgcag gcccggcacg ccggcccagt
     8161 ccgggtgatc gtggaacaac ccgcgcaggc gcgcgcacag ctgtgcagcc tcttccagcc
     8221 gggtgtcgtt gagcagcagc gcgaactctt cgccaccggt gcgtgccggc agatccgatg
     8281 cgcggcaggc cgcgccgatc accgccgcca cctgcaccag caccgcgtca ccgatgctgt
     8341 ggctgtgccg gtcgttgacg tccttgaagt ggtcgatgtc cagcaccacc aggcacaacg
     8401 agtggccact gcgctgcgaa cgggcgaaat cgcgggccag cgtttcgtcg aagccacggc
     8461 ggttggccag tccggtcagg gcatcctcgc gggcctggcg ctcaaacgct tcggcctgcc
     8521 gcgccaggcg ctcggccagc tcggtctttt cctggttcag ggcctgcagg tgcacggtct
     8581 tggcctgcag gtcgaaggtc gcctcgtcca cccgccgggc caggcgcagg ttgctggcct
     8641 tcagctgctg cagcagcagg cggtacagcg ccaccaggcc ggccagcagc aacaatccgc
     8701 ccagcgcctg cacgctgcgt cgctgccacc agaacggctc gacgctgaag ctccacaccg
     8761 cttcctgctg gccccagctg ccccccggat gtgccgccgc cacatgcagc gtgtagtcgc
     8821 ccggcggcag cccaacgaac tcgacgctgc gctgcgggcc gcgctcgacc cagccgctgt
     8881 ccaggccatc gagccgcgtg cggtagcgta tccggtcgga catcaggtag ctcaggccga
     8941 cgtagctgac cgccagccgg cgcccgcccg gaatgtggtt gcgttcgggg ccttcccagt
     9001 gcaccggcgc gccgtccacc tgcacgctct cgatcgccgc cggtggtgag gggcgctcgc
     9061 ggaaacgctg caggcgctgc gggtcgaccg tgctcaggcc gccggcggtg accacccaga
     9121 acgtgccgtc ctggcgcagg atcgccgacg gcccggagct gccattggcc tgcgcgttgg
     9181 ccatgccgtc gatctcgttg tagcgctcca ccaccactcg cggcgtgcgt ccatcggcca
     9241 ctgcattgag cgtggccatg tcggtgcgca gcacgccgcg gttgctgctg atccacacat
     9301 tgccgagccg gtccggcacc agctggaaca ccgcgtccac cggcatgccc tgttccaggc
     9361 cgacccgtgc caacgtcccg ttctgccagc agtacagacc gcggtcgctg ctgatccaca
     9421 tcgcatcgcc cagctggtgg aagccgaaca cgctgcggcc gccgcccagc ggggccaggt
     9481 tgatcgattg cacgcggtcg ccgcgcagta cgcggatgcc ttcgatggtg ccgatccaca
     9541 ggttgccctg atggtcgtgc tccagcgccg tgatcaggcc gcccggcatc cccggcacgt
     9601 tggagacctg cacccggtcg ccatcgatgc gtaccacgcc cttctgcgtg ccggcccaga
     9661 caacgccctg cggatccacg ctgattgcgc ggatgttgcc accgggcatg ccgtcggcgg
     9721 cggcgtagtt gtggcgcagg ctgccatcgc ggccaagccg gtagacgcca tcgccaaagg
     9781 tgccgaccca gaggtcgcca ttcggcccct gcgccaggct cagcaccgag ggcgccttgc
     9841 caccacggtt gtgcagcggc atcgcctgga aacgaccgtc ggcggtctgc cggtcaaggc
     9901 cactggcact gccgacccac agctggcggt cgcgatcctc caacaccgtg cgcacatagt
     9961 cgccgctgag cccatcgcgc tcggtatagc tgctgaacag cgtttcgcgc aggcggtaca
    10021 ggccaccgtt ggcgcctacc cagatgctgc cctcggcatc ttcgcgcagg ctcaccaccc
    10081 gcccgcccgg caggttcagg ccggccggca gccgttccag cccgtgcgcg gaaatgcgca
    10141 gcaggccctg gttctcggtg cccagccaca gatcgccgtg gcgatcctgc agcatcgcgg
    10201 tcatgtgcag ctggcccggc aggcgatgca ccagcaccag ccggtcatcc tccacccggt
    10261 acaggcgctc gccggcgacg atccacagcg caccatccgg cgcccggtac ggccacgaca
    10321 ggccgctgcc ggcacccagc ccccacgccg ccggcgcgcg cttgagcacg ccgtcgttgt
    10381 cgcgcagcac caggccgtcg agggtgccga tccagacccg gtcctgcgca tcgaccacca
    10441 gcttggtgaa gctcatcgcc atcggcagat cggccgctgg cgcctggtag accatgccgc
    10501 ggtcgggaag caggtaaccg atgcccttgc cttcatacaa cagccacagg cggccctggc
    10561 tgtccatctg catcgactgg atcagcacct gcggcgtatc ggcctggtgt tcccagaccc
    10621 gccactggcc gtcgctgcca cgatggctga cattaccgcg cgaatcgctg atccacagcc
    10681 cgccctggcg atcgacgaac agcgcgccga tgccgttgtc gcgcaggccc ggacgcgtgc
    10741 tgcggtcgaa cacggtgaaa tccaggccgt tgtagcgcac cagcccttcc caggtggcga
    10801 accataggtg gccttccggc gtctgcgcga tgtcgcgcag cgagttgtgc ggcaggccgt
    10861 tgcgcgaggt ccatac
//
