LOCUS       NZ_JALNLQ010000002     24235 bp    DNA     linear   CON 10-JUN-2024
DEFINITION  Lysinibacillus sp. Bpr_S20 NODE_2_length_699351_cov_151.83, whole
            genome shotgun sequence.
ACCESSION   NZ_JALNLQ010000002
VERSION     NZ_JALNLQ010000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Lysinibacillus sp. Bpr_S20
  ORGANISM  Lysinibacillus sp. Bpr_S20
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Lysinibacillus.
REFERENCE   1  (bases 1 to 699351)
  AUTHORS   Longdet,I.Y., Kutshik,R.J. and Okenmor,G.A.
  TITLE     Complete genome sequence of novel Lysinibacillus spp with high
            aromatic hydrocarbon degrading capacity
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 699351)
  AUTHORS   Longdet,I.Y., Kutshik,R.J. and Okenmor,G.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2022) Department of Biochemistry, University of
            Jos, Bauchi Road, Jos, Plateau State 930003, Nigeria
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 11-DEC-2020
            Assembly Method :: SPAdes v. DECEMBER-2020; CheckM's leanage_wf v.
            DECEMBER-2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023337835.1-RS_2024_06_10
            Annotation Date                   :: 06/10/2024 05:11:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,895
            CDSs (total)                      :: 4,826
            Genes (coding)                    :: 4,751
            CDSs (with protein)               :: 4,751
            Genes (RNA)                       :: 69
            rRNAs                             :: 7, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 54 of 75
            Pseudo Genes (internal stop)      :: 13 of 75
            Pseudo Genes (multiple problems)  :: 22 of 75
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:43:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 29168
            Orig. end                         :: 53403
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALNLQ010000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..24235
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="betalactone"
                     /region_number="1"
                     /rules="(HMGL-like and AMP-binding)"
                     /tool="antismash"
     cand_cluster    1..24235
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(HMGL-like and AMP-binding)"
                     /kind="single"
                     /product="betalactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..24235
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[10000:14235](+)"
                     /cutoff="10000"
                     /detection_rule="(HMGL-like and AMP-binding)"
                     /neighbourhood="10000"
                     /product="betalactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..14235
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(HMGL-like and AMP-binding)"
                     /neighbourhood="10000"
                     /product="betalactone"
                     /protocluster_number="1"
     misc_feature    1879..1888
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 17.8"
                     /tool="antismash"
     misc_feature    complement(2147..2161)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.58"
                     /tool="antismash"
     misc_feature    complement(2201..2215)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.81"
                     /tool="antismash"
     gene            2254..3120
                     /locus_tag="MWJ95_RS04790"
                     /old_locus_tag="MWJ95_04825"
     CDS             2254..3120
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225544.1"
                     /locus_tag="MWJ95_RS04790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_04825"
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="WP_249104920.1"
                     /transl_table=11
                     /translation="MDNQTIQQVIQHKYQELSKSQQKVAIFILKNLQTVGVHSAAYVGD
                     KAGVSETTVIRFCYAIGLSGYAELQREITMHLFNDGKASSLQNYLQSKQALFETPRFYE
                     KTMEKDASSILNVAKNINEEDFDRASYLLHTKKKVYIVGNGSSRLAAQWLHFTLNMLRP
                     NVVLLDFETSEIVRALQEIDEDSVVIILSFHRYFKEPIQFAAEIQGKNCEIIGITDSKI
                     APITQYATITFVNEQQEMSTIDAMPSLISFLNTLIAGMTAQDHDYYENQRIKYDDFQNS
                     FLANRWS"
     gene            3126..4241
                     /locus_tag="MWJ95_RS04795"
                     /old_locus_tag="MWJ95_04830"
     CDS             3126..4241
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1244: amidohydrolase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225542.1"
                     /locus_tag="MWJ95_RS04795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_04830"
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="WP_249104572.1"
                     /transl_table=11
                     /translation="MNQVLQNLQPRLADIFTHLHEHPEISWQEKKTTQYIANILKEAQM
                     EPQLFEDMTGLYVDIGEGIPRVGFRTDIDALWQEVDGKFQANHSCGHDGHMTMAIGTAL
                     VLKDMEHLLPGAVRILFQPAEEKAQGARAFVEKGLIDNLEYLFGVHVRPLKELQDGTYA
                     PALYHGAAKLFTGTIIGEEAHGARPELGINVIEVGAAIVEGLQKIWTDPNVSASVKMTQ
                     FNAGGASTNIIPGKATFSIDARAQTNEVMEAITIGVERVKASVEAMYGAKISLNVDAHI
                     VAALVNDEALQHMNSAIVDTVGEKACASPVVTPGGEDFHFYTYERPNLKATMLGLGCGV
                     TPGLHHPKMTFNRERLITGVNIITRAILRAL"
     gene            4279..5127
                     /locus_tag="MWJ95_RS04800"
                     /old_locus_tag="MWJ95_04835"
     CDS             4279..5127
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008405429.1"
                     /locus_tag="MWJ95_RS04800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_04835"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_349294136.1"
                     /transl_table=11
                     /translation="MRVLIDIREIKTIKQLEQVQQLEYNVWGMPSIPIHQTLTAVKNGG
                     IVVGAYDGDQLVGFSYGFSGFREGKSYLCSHMLGIDENYRSKGIGEQLKYAQQAIAIER
                     GYDLMVWTFDPLETRNGFLNLSKLNGICYTYIENCYGEMQDGLNKGLPSDRFEVCWYIT
                     SDYVSKNVSIDTDGAIPVASCVQNEQGFLRLEITENLKYDRDLYTLPVPKDFQALKLQD
                     PALALDWRFKTRHILQRLFAQGYAAIQLQQQEHYNEYVLVKLEILNLEGANQYENHGNY
                     H"
     gene            5102..6208
                     /gene="menC"
                     /locus_tag="MWJ95_RS04805"
                     /old_locus_tag="MWJ95_04840"
     CDS             5102..6208
                     /EC_number="4.2.1.113"
                     /GO_function="GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="menC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1268: mandelate racemase/muconate lactonizing enzyme"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225539.1"
                     /locus_tag="MWJ95_RS04805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_04840"
                     /product="o-succinylbenzoate synthase"
                     /protein_id="WP_249075261.1"
                     /transl_table=11
                     /translation="MKITEITIRHLKMKLKEPFTTSFGTFEDKDFLVLEAKDEAGTIGW
                     GESVAFHSPWYNEETLQTNWHMLEDFLIPLILNKELQHPDEVNELFKPIRKNNMAKSTI
                     EGAIWDIYAQQTNQSLAAALGGKKDNIEVGISIGIQKSIEDLVALVDGFVKEGYKRMKI
                     KIKPGWDVEVMRTLREAFPNVAFMADANSAYSLSDAETLKQLDAFNLTMIEQPLASDDI
                     IDHAALQKLIDTPICLDESIHSLEDARKAVELGSTKIINIKIGRVGGLTEAKKIHDYCE
                     ANNIPVWCGGMLESGIGRAHNVALTTLSNFILPGDTAGSSRYWEKDIIDPEVVVKDGYI
                     EVPQKAGIGYDVNRETIDMFTVAQKTYK"
     gene            6266..7318
                     /locus_tag="MWJ95_RS04810"
                     /old_locus_tag="MWJ95_04845"
     CDS             6266..7318
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121609.1"
                     /locus_tag="MWJ95_RS04810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_04845"
                     /product="hypothetical protein"
                     /protein_id="WP_249075260.1"
                     /transl_table=11
                     /translation="MKKSWQIGGAFVGLIVGAGFASGQEIMQYFTSFGLYGIAGGIVAT
                     IAFAFLGMSLAQLGANSQTTSHKEVIYQIGGRYVGAILDIVITFFLFGVAVVMFAGSGS
                     TFEQMFGINPMIGSVFMVIMTILTLLLNVKNIINIIALVTPYLMGIVFIILIYSIFTMD
                     LTIAEQNVLAKEQTSAASNWILGALLYVSYNIAAGTAMLIVMGGTVKERKIAGLGGFLG
                     GLMLGILIILINIALFVKMDVVGGTAMPTLELAKQIHPVVGILMSIALLGMMYNTAVGM
                     LYAFTVRFIAPDHQYFKIGIVVIGLIGFVASLVGFTTLVGKVYSTMGYLGFALIIAIII
                     SWLRKIPKAS"
     gene            7963..8613
                     /locus_tag="MWJ95_RS04815"
                     /old_locus_tag="MWJ95_04850"
     CDS             7963..8613
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018392430.1"
                     /locus_tag="MWJ95_RS04815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_04850"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_249104923.1"
                     /transl_table=11
                     /translation="MVKKTLKQRIVDASVVLFQQDGYHNVTVDRIVEYIGASKGGFYHN
                     FKSKDELLYEIHDVFISYVIKQSQEAYDKYDTPITRLCAMLQTLTKVFDMYQAHITVFY
                     EESRSLPEEYSEIIHEKRDQYRDILQKVIEEGQQTKDFRTELPCTIVTMAIVGMINWTY
                     KWFKQSGPLTMEEITEVFTDMVLRAIVTEQAMDEAVQFMVKGEPAVETGFINT"
     gene            8825..9967
                     /locus_tag="MWJ95_RS04820"
                     /old_locus_tag="MWJ95_04855"
     CDS             8825..9967
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864021.1"
                     /locus_tag="MWJ95_RS04820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_04855"
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="WP_249075259.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 7.5e-41, bitscore:
                     134.6, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 1.2e-27, bitscore:
                     91.3, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 4.5e-52, bitscore:
                     171.5, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNFELTKEQVMIRDMVRDFAENEIKPYAREVDETSTMRIESFKKM
                     AELGLLGIPFPEEYGGSGGDTVSYALAVEEIGRACGGTGLSYAAAVSLGASPIYNFGTE
                     EQKQEWLVPLAKGETLGSFGLTEPNAGSDAGGTRTTAVIDGDDYIINGEKCWITNAGYA
                     RQIIVTAVTGKREDGKKIISSIIVPTNTPGVTINCNYDKMGVRGSNTCEIILQNVRVPR
                     ANLLGDEKRGFSQFLNTLDGGRISIAALSVGIAQAAYEKALKYAKEREQFGAPISKLQA
                     IQFKLADMAMEIELARTLVHKAAWLKDQKKPFGKEAAMAKLFASEMGFRTCNQAIQIHG
                     GSGYMKEYDVERHLRDIKLMEIGEGTSEIQRLVISRLIGC"
     gene            10001..11635
                     /locus_tag="MWJ95_RS04825"
                     /old_locus_tag="MWJ95_04860"
     CDS             10001..11635
                     /NRPS_PKS="Domain: AMP-binding (13-445). E-value: 1.9e-101.
                     Score: 331.5. Matches aSDomain:
                     nrpspksdomains_MWJ95_RS04825_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     betalactone: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292658.1"
                     /locus_tag="MWJ95_RS04825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_04860"
                     /product="AMP-binding protein"
                     /protein_id="WP_249104575.1"
                     /sec_met_domain="AMP-binding (E-value: 1.1e-108, bitscore:
                     358.7, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAELVYQTIGQLLDEQSEKYPQREALVYADRDLRMTYEELNRQSR
                     LVARGLMALGIEKSEHIAVWTTNVPEWVQLQFATGKMGAPIVTVNTNYRSSELEYLLKQ
                     SDAKTIILIENYRDHSFMNTLQELCPELDHCEPGNLQSKRLPLLKNVILIGETKYPGVL
                     NWSDVLSAAEQITEEQLDQRQQALHYDDVINIQYTSGTTGFPKGVMLTHYNLVNNAINI
                     AECMRLTYEDRLCIPVPFFHCFGCVIGTLAITTSGGTMVPVQEFSPTEVLQTVEKEKCT
                     ALHGVPTMFISELNLPNFSSFDLSTLRTGVMAGSNCPIEVMKAVIDKMGMKDVTICYGQ
                     TEASPVITQTRADDPLLLKVETVGRALPNLEVKIVIPGTNEEAAPNEQGELCTRGYHVM
                     KGYYKNPEETLLAIDKDNWLHTGDLATMDEAGYVRVTGRLKDMIIRGGENLSPREIEEF
                     LYTHPKIADVQVAGVPDPVYGEEAAAWIILREGEQATEEEIRDYCRDKISRHKIPRHIF
                     FIDDYPMTASGKVQKYRLRESFGATVK"
     aSDomain        10040..11335
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MWJ95_RS04825_AMP-binding.1"
                     /evalue="1.90E-101"
                     /label="MWJ95_RS04825_AMP-binding.1"
                     /locus_tag="MWJ95_RS04825"
                     /protein_end="445"
                     /protein_start="13"
                     /score="331.5"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="DEQSEKYPQREALVYADRDLRMTYEELNRQSRLVARGLMALGIEK
                     SEHIAVWTTNVPEWVQLQFATGKMGAPIVTVNTNYRSSELEYLLKQSDAKTIILIENYR
                     DHSFMNTLQELCPELDHCEPGNLQSKRLPLLKNVILIGETKYPGVLNWSDVLSAAEQIT
                     EEQLDQRQQALHYDDVINIQYTSGTTGFPKGVMLTHYNLVNNAINIAECMRLTYEDRLC
                     IPVPFFHCFGCVIGTLAITTSGGTMVPVQEFSPTEVLQTVEKEKCTALHGVPTMFISEL
                     NLPNFSSFDLSTLRTGVMAGSNCPIEVMKAVIDKMGMKDVTICYGQTEASPVITQTRAD
                     DPLLLKVETVGRALPNLEVKIVIPGTNEEAAPNEQGELCTRGYHVMKGYYKNPEETLLA
                     IDKDNWLHTGDLATMDEAGYVRVTGRLKDMIIR"
     CDS_motif       10583..10636
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MWJ95_RS04825_0001"
                     /evalue="1.30E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="MWJ95_RS04825"
                     /protein_end="212"
                     /protein_start="194"
                     /score="24.0"
                     /tool="antismash"
                     /translation="IQYTSGTTGFPKGVMLTH"
     CDS_motif       11162..11227
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MWJ95_RS04825_0002"
                     /evalue="1.10E-04"
                     /label="NRPS-A_a6"
                     /locus_tag="MWJ95_RS04825"
                     /protein_end="409"
                     /protein_start="387"
                     /score="14.6"
                     /tool="antismash"
                     /translation="QGELCTRGYHVMKGYYKNPEET"
     gene            11651..13009
                     /locus_tag="MWJ95_RS04830"
                     /old_locus_tag="MWJ95_04865"
     CDS             11651..13009
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1090: acetyl-CoA carboxylase biotin carboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225534.1"
                     /locus_tag="MWJ95_RS04830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_04865"
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="WP_249104578.1"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 2.8e-13,
                     bitscore: 45.1, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp (E-value: 1.6e-08, bitscore:
                     29.5, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 2.9e-06, bitscore: 22.3,
                     seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKKVLIANRGEIARRVIRTCKRLNVQTVAVYSEADAESLHVKDA
                     DEAFCIGKPPVAQSYLNIDRILEVAKESGADAVHPGYGLLSENAEFAKRCTEAGLVFIG
                     PSADVIASMGSKLEARKTMKAAGVPIVEGVEAPLKDVADAIEIASRLGYPIMLKASAGG
                     GGIGMQLVENAEELAKAFEGNQKRAQSFFGDGTMYMERFIANPHHVEVQIMADHDGNVV
                     PLFERECSIQRRNQKVVEEAPSPFISEETRKRMLDASVKAVQHIGYVNAGTIEYLVDAE
                     QNFYFLEMNTRLQVEHPVTEEITNLDLVEAQLKIAAKQPLAFTRDSIQRKGHAIEVRIY
                     AENPSTFFPSPGTITAFELPTGEGIRHECGVEAGTTVTPFYDPMISKLVVWGETRTIAC
                     ERLIEALQAYKVEGIQTNIPMLLKTVTHEQFLKGYTTTKFVDEYYLPQLAETK"
     gene            13090..13302
                     /locus_tag="MWJ95_RS04835"
                     /old_locus_tag="MWJ95_04870"
     CDS             13090..13302
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992214.1"
                     /locus_tag="MWJ95_RS04835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_04870"
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="WP_249075256.1"
                     /transl_table=11
                     /translation="MATVKASMAGTVWKIVVAEGEKVTAGQDVAILESMKMEIPIAAEE
                     DGVVTKIIASEGDFINVDDDILEIE"
     gene            13318..14235
                     /locus_tag="MWJ95_RS04840"
                     /old_locus_tag="MWJ95_04875"
     CDS             13318..14235
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     betalactone: HMGL-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1271: 2-isopropylmalate synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292662.1"
                     /locus_tag="MWJ95_RS04840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_04875"
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="WP_249104580.1"
                     /sec_met_domain="HMGL-like (E-value: 4.9e-53, bitscore:
                     175.6, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQLPKHVTLKEVGPRDGLQNEKTHIATADKVHLVNLLSQTGLKYI
                     EVTSFVHPKWIPQLADAVEVLQAIKRQKDITYAALVPNMRGLERALQAEVDEVSVFMSA
                     SESHNQSNINKSINETFPILQEVVVGAKAAYKNVRGYISTVIGCPYEGYIHPEKVLRVT
                     EKLFEMGVDEISLGDTIGVGVPTQVENLLEELLKRYPAENFAMHFHDTRGTALANIVQS
                     LEMGITKFDSALGGLGGCPYAKGASGNVATEDLLYLLDEMGIKTGVERNKVLEAALFIE
                     QKLGKDVASKQMAIVRNERSASQL"
     gene            14232..15014
                     /locus_tag="MWJ95_RS04845"
                     /old_locus_tag="MWJ95_04880"
     CDS             14232..15014
                     /EC_number="4.2.1.17"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: ECH (13-252). E-value: 1.5e-67. Score:
                     219.5. Matches aSDomain:
                     nrpspksdomains_MWJ95_RS04845_ECH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023: enoyl-CoA hydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292663.1"
                     /locus_tag="MWJ95_RS04845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_04880"
                     /product="enoyl-CoA hydratase"
                     /protein_id="WP_249104581.1"
                     /transl_table=11
                     /translation="MTQLVRFEVVDHCIGLITLSRPEAANAMSIQLLQELSTTLDNING
                     DPAVRVVLLTGAGEKAFCAGADLKERKGMPDRQVKQIVQLIGATVAKVEALAQPVIAVL
                     NGVAFGGGLELALACDLRLAAAHSKIGLTETSLGIIPGAGGTQRLPRLIGIGKAKELIY
                     TARRLNAEEARSYGIVEYVYEGHEILEKAQQLALEMAKNAPLSLVQAKIAMNQGVEVDL
                     ATGLKIESLAYNALIPTEDRLEGLLAFQEKRTPQYVGK"
     aSDomain        14271..14987
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MWJ95_RS04845_ECH.1"
                     /evalue="1.50E-67"
                     /label="MWJ95_RS04845_ECH.1"
                     /locus_tag="MWJ95_RS04845"
                     /protein_end="252"
                     /protein_start="13"
                     /score="219.5"
                     /tool="antismash"
                     /translation="IGLITLSRPEAANAMSIQLLQELSTTLDNINGDPAVRVVLLTGAG
                     EKAFCAGADLKERKGMPDRQVKQIVQLIGATVAKVEALAQPVIAVLNGVAFGGGLELAL
                     ACDLRLAAAHSKIGLTETSLGIIPGAGGTQRLPRLIGIGKAKELIYTARRLNAEEARSY
                     GIVEYVYEGHEILEKAQQLALEMAKNAPLSLVQAKIAMNQGVEVDLATGLKIESLAYNA
                     LIPTEDRLEGLLAFQEK"
     gene            15492..17030
                     /locus_tag="MWJ95_RS04850"
                     /old_locus_tag="MWJ95_04885"
     CDS             15492..17030
                     /EC_number="6.-.-.-"
                     /GO_function="GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292664.1"
                     /locus_tag="MWJ95_RS04850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_04885"
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="WP_249075253.1"
                     /transl_table=11
                     /translation="MSNVPTENTTKTMKEQILAGGLPKYHDKNAQQGKLFVRERLELLL
                     DDGLQSEDGLYANCLAGDLPADGVVTGIGKIHGRTVCVLANDSTIKAGSWGKRTVEKMI
                     RIQETAEKLNCPLLYLVDSAGARITDQLEMFPGRRGAGRIFYNQVKLSGKIPQICLLFG
                     PSAAGGAYIPAFCDIVVMVEGNASMYLGSPRMAEMVIGEKVDLETMGGAKMHCSVSGCG
                     DVLAKTEQEAIAYARKYLSYFPNNYAERSKVEAPKPPAIFDKSIDELIPKNQNVPFDMY
                     KLIERIIDEDSFCEVKKLFAPELITGLGRINGQSVGIIANQPRVKGGVLFHDSADKAAK
                     FISLCDAFNIPLLFLADVPGFMIGTQVEKAGIIRHGAKMIFAMSEATVPKLTVIVRKAY
                     GAGLYAMAGPAFEPDCCIALSNAQIAVMGPEAAVNAVYANKIAELPKEEQASFIAEKRK
                     EYQEEIDVYRLASELIIDDVIEPNDLRKALESRLELYMSKYLLFSERKHGVNPV"
     gene            17227..17781
                     /locus_tag="MWJ95_RS04855"
                     /old_locus_tag="MWJ95_04890"
     CDS             17227..17781
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_process="GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /GO_process="GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176113.1"
                     /locus_tag="MWJ95_RS04855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_04890"
                     /product="VanZ family protein"
                     /protein_id="WP_249104584.1"
                     /transl_table=11
                     /translation="MAKNNIQGSAKLPLPYRSLIDTYYLPIKVTGWIFFCIYSVIAFYK
                     LVIDRLVNVVVILFHGEYSLGDLGLFDYSNWQLTTNFVPFDTILRYINYSQYFNFDIII
                     INLLGNLLIFTPMGFLLPLLSKKCRKAWKVICIGFFSSLAVETIQFIFRVGSADIDDLI
                     LNTLGAWLGYLAYKSILIKNR"
     gene            18026..18766
                     /locus_tag="MWJ95_RS04860"
                     /old_locus_tag="MWJ95_04895"
     CDS             18026..18766
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225526.1"
                     /locus_tag="MWJ95_RS04860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_04895"
                     /product="hypothetical protein"
                     /protein_id="WP_249104586.1"
                     /transl_table=11
                     /translation="MKLSSLIELERMSSQYNRKKILQRKQADDAYRKNSLYFQPKKNPL
                     LLELENLLLGRTDQDLYEQQKEESKEVTPRQQTIMQDLQQTKKNVLAHEQTQKAEALEY
                     SQIEDLDEEAPFGDDVNANELFQTLEKVRTAALAPAQPSPQDLRVAASANAQLLHMQGQ
                     LNDQELDEREPSFVRETIEVKVPERFLKELKLDPFADTIFGKSYAEAYKARTFKYASEK
                     YATHIQMAKDGYQPGNDPKFSMIA"
     gene            18792..19286
                     /gene="ybaK"
                     /locus_tag="MWJ95_RS04865"
                     /old_locus_tag="MWJ95_04900"
     CDS             18792..19286
                     /GO_function="GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /GO_process="GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="ybaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225525.1"
                     /locus_tag="MWJ95_RS04865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_04900"
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="WP_249104587.1"
                     /transl_table=11
                     /translation="MAKAKHAKTNAIRLLEQQKIQFEVIEYETGDGQVDGISVAEKIGH
                     PVSRVFKTLVAKASAQKLFVFVIPVADELDLKAAAKVVGEKKIDMLPVKELLGYTGYVR
                     GGCSPVGMKKLYPTIIDASAQEQEEIIVSAGKIGMQIHVQLDDLVAVTKAKLAPITTTQ
                     A"
     misc_feature    complement(19577..19590)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.54"
                     /tool="antismash"
     misc_feature    complement(19578..19591)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.41"
                     /tool="antismash"
     gene            19705..20286
                     /locus_tag="MWJ95_RS04870"
                     /old_locus_tag="MWJ95_04905"
     CDS             19705..20286
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014824845.1"
                     /locus_tag="MWJ95_RS04870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_04905"
                     /product="glycosidase"
                     /protein_id="WP_249075290.1"
                     /transl_table=11
                     /translation="MLIVSTSTLFIEALQIAIRDRRSTWQVDTYQIQTPIFDQTLMKKI
                     KEQCLDIVVVEATNQHFTMTIDSLKKLKCPEIDIMLLVDSRMGEVYHHLKDEERWTSVT
                     KNTSLDEFLILLESFGCNIPDLPDVSLREVDKKILKELAVGHSFEFIQRTQELSAEEID
                     QSLYRINTYFKVPNYIESIVKAFEQKIITN"
     gene            20400..21287
                     /locus_tag="MWJ95_RS04875"
                     /old_locus_tag="MWJ95_04910"
     CDS             20400..21287
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406307.1"
                     /locus_tag="MWJ95_RS04875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_04910"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_249075249.1"
                     /transl_table=11
                     /translation="MTFKIENLNKSFGDKKAVNNISIRLEEGQILGMLGRNGAGKTTTI
                     RMMLELIPKDSGQILWKGKPFSKKNLKIGYLPEERGLYAKMNVLDQIIYFGMLEGMDKK
                     EAKREALKWLEKLEISYAAKKRTEQLSKGNQQKVQLITAIIHDPEFIILDEPFSGLDPV
                     NADMLKSVVKELIAQKKTIIFCSHQMDQVESFCNQICILKDGDLIVSDQLSAVKKSYDY
                     RYLELDTTANIKDYLALKQYKFTQENNRYKLRIPRNDKIHQTIREIDEKFDLQGVSLKE
                     PSLHEIFIERTGGA"
     gene            21288..22493
                     /locus_tag="MWJ95_RS04880"
                     /old_locus_tag="MWJ95_04915"
     CDS             21288..22493
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406308.1"
                     /locus_tag="MWJ95_RS04880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_04915"
                     /product="ABC transporter permease"
                     /protein_id="WP_249104589.1"
                     /transl_table=11
                     /translation="MRNFSIVFNNVFKEAVKTKAYLYMTIGLVGLSLLLFASPSIMKVF
                     TSNSPDETYKIVNQTSLELSEKELTGLAGSWTIVENANIEDLKEDIKTKELTGFFVLED
                     QTNVMQLNIYMPKVNNEVLLTLESYIKQKKVASIVAKQSIDTGVVQELNTPLQSSVQSL
                     YETEPNALFIIYGLITIMFFSISIYGNNVATAIASEKSSRVMEVMITKVAPVPMMYGKI
                     LGIGLASLTQLFIFMGALLLWANVGIFEVPEGSFVQAVMETITWGTAGYILIFCTIGYF
                     IYATLYAIVGSMISRPDDLASASMPIMIILMASLVVEMLFVVDSPEGTMTNITSYIPFT
                     APTSVLIRIVYNTISPIEIAVSLLVMISFIALFAAVAAKIYPKGVLKSDSLTFKQLIQF
                     NR"
     misc_feature    complement(21312..21323)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.34"
                     /tool="antismash"
     gene            22793..24094
                     /locus_tag="MWJ95_RS04885"
                     /old_locus_tag="MWJ95_04920"
     CDS             22793..24094
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406309.1"
                     /locus_tag="MWJ95_RS04885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_04920"
                     /product="GHKL domain-containing protein"
                     /protein_id="WP_249104591.1"
                     /transl_table=11
                     /translation="MDVLTGKMLLMSIIQFSMMLFFCTYILDLYWSKKQLITIIIIIFL
                     PTFLLFPFIGAWSMLYCLSLLAIMMYRETKVVMNILHVCMALILLVIGDNISNILTFRL
                     FNGIGNEQLSIISYFFFYIVFGLIFAFCYKRIVKYLISRWVINSFVSYISVFLGITTVV
                     FMYINIMAIDYDNFYESVQNNLAMFLIYFVLLSISVFVVLYLAFKQYKMKQREQEMKNF
                     ESYVTSIEQINQDMRKFKHDYINILSSLRTFIDDKNYEGLHTYFYDHILEANHHEQLNQ
                     QAMMMLNNLKINSLKGLVTTKILQAQSHHVPFYIEIVEEITDIDVDPILLNRMVGILLD
                     NGIEAARGVENGEVRMAFIHMGEVVLLVVSNTFDKKIAIKVHEIYQEGFSTKGEERGLG
                     LANLRQMTNDLHNVNLNTKISPPYFIQEVGFRKG"
     misc_feature    24205..24218
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.91"
                     /tool="antismash"
ORIGIN
        1 acgtgggcat tagggcattt agtaacgcta gccgatccag agagctacga tgaaaaatat
       61 aaaaaatgga atttagaaga cctaccgatg ctaccagagc gtctaaaatt aacaacgatt
      121 aaacagacga gcaaacaata caatgcagta aaatcacagc ttactcgtaa cgatgtaaac
      181 gaaattatta ttgcaacaga tgctggtcgt gagggtgagc ttgtagcacg ttggattatt
      241 gaacgtgcga aagtaaataa gccgattaaa cgtttatgga tttcatctgt tacagataaa
      301 gccattaaag aaggatttgc taatttaaaa ccaggtaaaa actacgataa cctatatgct
      361 gctgcggttg ctcgttctga agcggattgg tatatcggat taaacgcgac acgtgcatta
      421 acgacaagat ttaatgcaca gctaaactgt ggccgtgttc aaacgccaac tgttgcgatg
      481 atagctgcac gcgaagatga aattaaaaac tttaaaccac aaacgtacta cggtattgaa
      541 gcacaaacag attcgttaaa gcttacatgg caagatgcaa atggtaattc acgtagtttc
      601 aacaaagaaa aagtggacgt tattgtcaaa gcactaggca atcaagatgc aaaaattgct
      661 tcaattgatc gcaagccgaa aaaatcattt gccccaggtc tttatgattt aacagaatta
      721 cagcgagatg ccaataaact atttggctat tcggcaaaag aaacattaaa tattatgcaa
      781 aagctatatg aatctcataa agtgctaaca tatccgcgaa cagattcacg ttatttatca
      841 tctgatatta taggcacatt acctgagcgt ctaaaagcat gtggcatcgg cgaatatcgt
      901 acattaacga ataaaatttt aacaaagccg attaaagcga ataaatcatt tgtggatgac
      961 agtaaagtgt ctgatcacca cgcgatcatc ccaactgagg gctatgtgaa cgtatctgca
     1021 ttcaatgata aagagcgtaa aatatatgat ttagtagtaa aacgattttt agctgtgtta
     1081 ttcccagcgc aagaatatga gcaattaacc gtccaagcac aaatcgggca tgagaaattt
     1141 attgctaaag ggaaaaccgt tatcaatgct ggttggaaag aagtatatca aaatcgtttt
     1201 gatgatgagg aatcaacgga tgatgtcaaa gaacaactgc ttcctcgttt agaacaagga
     1261 caagtattaa aaacaaagct aattgcccaa acatctggtc aaacaaagcc acctgcacgt
     1321 tttaccgagg caacattact atcagcgatg gaaaatccta cgaagtatat ggagacgaac
     1381 gacaagaagc ttgcagatac gttgaaatca actggaggat tagggacagt tgcaacacgt
     1441 gcagatatta tcgaaaagct atttaattca ttcctaatcg aaaaacgtgg cggcaaagaa
     1501 atttacatta catctaaagg tcgtcagttg cttgatttag tgccagaaga attacgctct
     1561 ccagcgacaa cagccgaatg ggagcaaaag cttgagttaa tttcaaaagg taagctgaaa
     1621 aaagatgtgt tcatcaacga aatgaaaaag cacacgaaag aaatagttac agaaattaaa
     1681 gcgagtgata aaaaatataa gcatgacaat atctcaacaa aatcctgccc tgattgtggc
     1741 aagccaatgc ttgaagtcaa cggtaaaaaa ggcaagatgc ttgtttgcca agatcgtgaa
     1801 tgtggtcacc gtaaaaacgt atcacgtgtc acaaacgcaa gatgtccaca atgtaagaaa
     1861 aagctagaac tacgcggtga aggcgacggc caaattttcg tatgtaaatg tggttatcgt
     1921 gaaaagttat ctgcatttga agcccgacgt aaaaaagagg gcggcggcaa agtagataag
     1981 cgcagtgtac aaaaatacat gaaacagcaa gaaaaagaag cagaaccttt gaacaatgct
     2041 tttgcagatt tgttaaaagg gatgaagttt gatgaatagt tataaataaa aaaagcggga
     2101 gtgttaaata aattaacatt cccgcttttt tgttcgttat aaacagaaat ttcagaaaat
     2161 atattgaatt aagaaaataa gtatgctact gtatgtaaca tgaaaatgaa gtttaaactt
     2221 caaaaaataa aatgagaagg atgtacttct ttgatggaca atcaaacaat tcaacaagtc
     2281 attcaacata aatatcagga actttcaaag agtcagcaaa aggtagcgat cttcatcctc
     2341 aaaaatttgc agactgtcgg tgttcattct gctgcatatg tcggggacaa ggccggggta
     2401 agcgaaacga cggttatccg cttttgctac gcgataggat tatcgggtta tgctgagttg
     2461 cagcgggaga tcacgatgca tttattcaat gacggaaagg caagtagctt gcaaaattat
     2521 ttgcaatcca agcaggcact atttgaaacg ccacgttttt atgaaaaaac gatggaaaag
     2581 gatgcatcga gtattttaaa tgttgctaaa aatattaacg aggaagattt tgatcgtgct
     2641 tcgtacctac tgcatacgaa gaagaaagtc tatattgttg gcaatgggtc ctcccgttta
     2701 gcggcacaat ggctgcattt tacattaaat atgctacgtc ctaacgtagt tcttttagat
     2761 tttgaaacta gtgaaatcgt tagggcttta caagaaattg atgaggatag tgtggtcatt
     2821 attttatcgt tccatcgtta ttttaaagaa ccgatacaat ttgcggctga aattcaaggg
     2881 aaaaattgtg agattatcgg tattacagat tctaaaattg cacctatcac gcaatatgca
     2941 acgataacct ttgtgaatga acaacaagaa atgtctacga ttgacgcaat gccatcattg
     3001 atctcatttt taaatacact aattgcaggt atgactgcac aagatcacga ctattatgaa
     3061 aatcaacgaa taaaatatga tgatttccaa aatagctttt tagcaaatcg atggagttga
     3121 atactatgaa tcaagtttta caaaacttac agccgagatt ggctgatatt tttacacatc
     3181 ttcatgagca tccggaaatt agctggcagg aaaagaagac aacgcaatat atcgccaata
     3241 tattgaagga ggcacagatg gaacctcagt tgtttgagga tatgacggga ttgtatgtag
     3301 atattgggga agggataccg agagtcggat ttcgtacgga tattgatgca ctatggcagg
     3361 aagttgatgg caaatttcaa gctaatcatt cttgcggtca tgatggtcac atgacgatgg
     3421 ctattggaac ggcactggtg ttaaaggaca tggagcattt actccccggc gcagtacgta
     3481 ttctttttca gcctgctgaa gaaaaggcac agggtgctcg tgcattcgtt gagaagggac
     3541 ttattgataa tctagaatac ttattcggtg tccatgttcg cccgttaaaa gaattacaag
     3601 acggaacgta tgccccagca ttgtaccatg gtgcggcaaa gctttttact gggaccatta
     3661 ttggggaaga ggcacatggc gcaagacctg aactcggtat taacgtcatt gaggtagggg
     3721 ccgcaattgt cgaagggtta caaaaaatat ggacagaccc caacgtctct gcgtcagtca
     3781 aaatgacaca atttaatgca ggaggagctt ctacaaacat tattccggga aaagcaacat
     3841 ttagtattga tgcacgagca caaacaaatg aagtaatgga agcaatcacg attggtgttg
     3901 aacgtgtaaa ggcatcggtg gaagcgatgt atggtgccaa aatttcttta aatgtagatg
     3961 ctcatattgt tgctgcgtta gtgaatgatg aggcattaca acatatgaat tcagctattg
     4021 tcgatacggt tggtgaaaaa gcatgcgctt caccagtcgt cactcctgga ggagaagatt
     4081 ttcattttta tacgtatgag cgtccgaacc ttaaagcaac aatgcttggc ttaggttgtg
     4141 gtgtaacacc aggattacac catccgaaaa tgacgtttaa tcgagagcgg cttattacgg
     4201 gagtgaacat tatcacgaga gctattttac gggctttata ggccctagcc atcaataggg
     4261 gataaagaat agaaagaagt gagagtattg attgacataa gggaaataaa gacaattaaa
     4321 cagctggagc aagttcagca attggaatat aacgtttggg gaatgccttc aattcccatt
     4381 catcaaactt taacggctgt aaaaaatggc ggtatcgttg ttggtgccta tgatggtgat
     4441 caattggttg gctttagcta tggcttttca ggatttcgag agggcaaaag ctatttatgt
     4501 tcccatatgt tggggataga cgaaaattac cgctccaaag gaataggtga acaattaaaa
     4561 tatgcccagc aagctattgc tattgagcgg ggctacgatt taatggtgtg gacgtttgat
     4621 ccgctggaaa caagaaatgg ttttcttaat ctgtcaaaat taaatggtat ttgttatacg
     4681 tatatagaaa actgctacgg tgaaatgcag gatggattaa ataaaggact cccttcagat
     4741 cgttttgagg tgtgctggta tataacctct gattacgtaa gtaaaaatgt ttcaatagat
     4801 acagatggcg ccataccagt tgcaagctgt gtacaaaatg aacagggatt cctacgttta
     4861 gaaataactg aaaatttaaa atacgataga gatttatata cgctacctgt accaaaggat
     4921 tttcaagcat taaagctgca agacccggca ttagcattgg actggcgctt taaaacgcga
     4981 catattttac aacggttatt tgcacaaggt tatgcggcta ttcaattgca gcagcaagaa
     5041 cattacaacg aatatgtcct cgtaaaatta gaaattttaa atctggaagg agcaaaccaa
     5101 tatgaaaatc acggaaatta ccattagaca tttaaaaatg aaattaaaag aaccatttac
     5161 aactagcttt ggcacatttg aggataaaga ttttttagtt ttagaggcaa aggatgaagc
     5221 aggaacaatc gggtgggggg aatctgtagc gttccattct ccttggtaca atgaggaaac
     5281 attacaaacg aactggcata tgctggaaga ttttttaatt cctcttattt taaataaaga
     5341 gcttcagcat ccggatgagg tcaatgaatt atttaaacca attcgcaaaa ataatatggc
     5401 gaaatcgact attgaaggtg caatctggga tatttatgcc caacagacga atcaatcact
     5461 ggcagctgca cttggcggca aaaaggacaa tattgaagta gggatcagta tcggtattca
     5521 aaaatcaatt gaggacttag tagcgttagt agatggtttt gttaaagaag gctataaacg
     5581 catgaaaatc aaaatcaagc caggttggga tgtagaagta atgcgtacat tacgagaagc
     5641 gttccctaat gtggcattta tggcggatgc gaactcagct tatagcctgt cagatgctga
     5701 aacacttaag caactagatg cttttaatct aactatgatt gagcagccat tagcgtcaga
     5761 tgacatcatt gaccacgcag cgttgcaaaa attaatcgat acgccaatct gcttggatga
     5821 aagtatccat tcattggagg atgcacgtaa agctgttgag ttaggcagta ctaaaattat
     5881 taacattaag attggtagag ttggtggcct aacagaagcg aagaaaatac atgattattg
     5941 tgaggcaaat aacattccag tttggtgtgg aggcatgctg gagtctggta ttggtcgagc
     6001 acataatgtg gcgttaacta cattatcgaa ttttatttta cctggagata ctgcaggctc
     6061 aagtcgttat tgggaaaagg atatcattga cccagaagtt gtagtgaagg acggctatat
     6121 tgaggttcct caaaaagcag gtattggcta tgacgtaaat agagagacga tcgatatgtt
     6181 cacggttgca cagaaaacat ataaataaga agtatatttc gttaggaagc ctatgaatat
     6241 aattgattag ataagcaggt gttctatgaa aaaaagttgg cagataggtg gcgcatttgt
     6301 aggattaatc gttggtgctg gttttgcttc aggacaggag attatgcaat actttacgag
     6361 ctttggcctt tacggtatag cgggaggaat tgtggccaca attgcatttg cctttttagg
     6421 gatgagttta gcgcaattag gtgcaaattc acagacaaca tctcataaag aggttatcta
     6481 tcaaattggt ggacgttatg ttggggctat attagatatt gtcattacat tcttcttatt
     6541 tggtgtagca gttgtaatgt ttgctggatc ggggtcaaca tttgagcaaa tgtttgggat
     6601 aaacccgatg attggcagtg tttttatggt catcatgacg attttaacat tgctattaaa
     6661 cgttaaaaat attattaata taatagcatt agtgacacca tatttaatgg gaattgtgtt
     6721 tattatttta atttactcca tatttacaat ggatttaaca attgcagaac agaatgtgtt
     6781 agcgaaagag caaacatctg cggcatctaa ttggatacta ggtgcacttc tctacgtatc
     6841 ttataacata gctgctggta ctgcgatgtt aatcgttatg gggggaacag taaaagagcg
     6901 taagattgcc ggtttaggcg gttttcttgg tggtttaatg ctcggtattt taatcatact
     6961 tattaatatt gccttgttcg ttaaaatgga tgtggttggt gggacggcga tgccaacatt
     7021 agagctagca aaacagattc atcctgtagt gggtatctta atgtctattg ctttattagg
     7081 catgatgtac aatacagctg taggaatgct ttatgcattt acagtacgct ttatagcgcc
     7141 tgaccatcaa tattttaaaa taggaatcgt ggtgattggt ttaataggct ttgtggcgag
     7201 tttagtaggg ttcacgacat tagttggaaa ggtatattca acaatggggt atttaggctt
     7261 tgcattaatc attgcgatta tcatctcgtg gctacgtaaa ataccaaaag cttcataact
     7321 atgttttgag attctctagt gtttttgtac agcgctaggg aatttttttg caattttagt
     7381 cataaaaata aggcgtgaaa catacaataa ttgtatgttt gttcattcgg gatgttttct
     7441 gaaattctgc tttatgtaaa ataaattctc gtaattttaa ataaaatcta ttttgttgct
     7501 agacactaat ttttctgggt gctatattaa tggtgtcata gttttttaat aattaggaaa
     7561 ataaagggtc gtcataatgt taaaacttac gttatagtga tgtgccatac aagtgatttc
     7621 taataaattg ttccaacatc ggaagtttgg gatgacattt tcttaaaacg tataccatgt
     7681 aaataagttg attggagtgg aggctgggcg actccttggg gatcagcgat aggggaacga
     7741 aggctaaaag catcacatcc tgtgataacg ccttcgtgac caacatcgtg ttggcccgag
     7801 accctgcagc gaagcggatg ttttctgtgc gaaagcgaag cggcagcaac aaagcgccca
     7861 gccggaacgg aaatcaacca cacattatgg ccatgaccta aaaccttttc taattataag
     7921 aggtgatgga gtaaagagta ataagaagga tgatagatgc ctatggtaaa gaaaacatta
     7981 aaacaacgta ttgttgacgc ttctgtagtg ctatttcagc aagatggcta tcacaatgta
     8041 actgttgatc gcattgttga atatattggt gcatcaaagg gtggatttta tcataatttt
     8101 aaatcgaaag atgaattgtt gtatgaaatt cacgatgtat ttatttcata tgtgattaaa
     8161 cagtcacaag aggcatatga caaatacgat acaccaatta cacgtttatg tgcgatgctg
     8221 caaacactga ccaaagtatt tgatatgtat caagcacata ttactgtttt ttatgaggaa
     8281 agccgttcat tacctgaaga atatagtgaa attattcatg aaaaacgaga tcaatatcgg
     8341 gatattttac aaaaagtcat agaggaaggc cagcaaacaa aggatttccg tacagaacta
     8401 ccttgtacta ttgtaacgat ggctattgtg gggatgatta actggacata taaatggttt
     8461 aagcaatcgg gtcctttaac aatggaggaa attacagaag tctttacgga tatggtattg
     8521 cgtgcgattg tcactgaaca agcaatggat gaagccgttc aatttatggt caagggggag
     8581 ccagcagtag agactggttt tattaatact taaaacaaac cgactagtcg gtattaactt
     8641 ctttcaaccg gtctcaaccc ccgttgaaag agaggatgcg ttagcactac gctttcgcgc
     8701 aaaaaacatt gtgttggccc aaagccgatt ccggacgcaa ttatgccgag gcataattga
     8761 ttttttacaa ctatcatctg aaacgaaatc cgctatggca aattaaacat tagggggact
     8821 ggggatgaac tttgaactaa cgaaagagca agtgatgatt cgagacatgg tgcgtgattt
     8881 tgctgaaaat gaaattaaac cgtatgctcg tgaggttgac gagacatcga cgatgagaat
     8941 agagagcttt aaaaagatgg cagagcttgg cttattagga atcccgtttc ctgaagagta
     9001 cggtgggtca ggtggagata cggtatccta cgcacttgct gttgaagaaa tcggtcgtgc
     9061 atgtggcggt acaggattaa gctatgcagc agcagttagc ctaggtgctt cgcctattta
     9121 caattttggt acagaggagc aaaagcagga atggctagtt ccgttagcga aaggggaaac
     9181 attaggttcg tttggcctaa cagagcctaa tgcaggttca gatgctgggg ggacacggac
     9241 aacagccgta attgatggtg atgattacat cattaatggt gaaaaatgct ggatcacaaa
     9301 tgctggctat gcacgtcaaa ttattgtgac agcagtgaca ggtaaacgtg aagatgggaa
     9361 aaaaattatc tcatctatca ttgtaccaac taacactcct ggcgtaacga tcaactgcaa
     9421 ttacgacaaa atgggcgtac gtggctccaa tacgtgtgag attattttgc aaaacgttcg
     9481 tgtaccaagg gctaatctat taggggatga aaaacgcgga tttagccaat ttttaaatac
     9541 gttagatgga gggcgtattt ccattgctgc attatctgta ggaattgcac aggcggctta
     9601 tgagaaagca ttaaaatacg caaaagaacg tgaacaattt ggtgcaccca tatcaaaact
     9661 tcaggcaatt caatttaaat tagccgatat ggcaatggag atagagcttg cgcgtacgtt
     9721 agtacataaa gcggcatggc ttaaagatca aaagaaacca ttcgggaagg aagcggcaat
     9781 ggcaaaatta tttgcttctg aaatgggctt ccgtacttgt aatcaagcca ttcaaattca
     9841 tggtggttca gggtatatga aagaatacga cgtagaacgt catctgcgtg acattaagtt
     9901 aatggaaatc ggtgagggaa cgtctgaaat tcaacgtctc gttatttctc gtctaattgg
     9961 ctgttaatca tcttaagtag cataaataag gaggattttc atggcagaac ttgtgtatca
    10021 aactattggt caattgctag atgaacaatc tgaaaagtat ccacagcggg aagcattagt
    10081 gtatgcagac agggatttac gaatgaccta cgaggagctc aatcggcaaa gtcgtttggt
    10141 agctagaggg ttgatggctc ttggaataga gaagagtgag catattgcag tatggacgac
    10201 caatgtcccg gaatgggtgc agctacaatt tgctacaggt aaaatgggtg ctccaatcgt
    10261 cactgtaaac actaattatc gttcaagcga gttggaatat ttattgaagc aatccgatgc
    10321 aaaaacgatt attttaattg aaaattatcg agaccattct tttatgaata cactgcaaga
    10381 gttatgtcca gagcttgatc attgtgaacc gggtaatttg caatcaaagc gactaccctt
    10441 attgaaaaat gttattttaa ttggcgagac aaaataccct ggcgtgctaa attggtctga
    10501 tgtgttatct gctgcagagc aaataactga agagcaatta gatcaaagac agcaggcact
    10561 tcattatgat gatgttatta atattcaata tacatcggga acaacaggtt ttccgaaagg
    10621 tgttatgtta acgcattata acttagtcaa taatgcgatt aatattgcag aatgtatgcg
    10681 tttaacttat gaagatcgct tgtgcattcc tgtcccattc ttccattgct ttggctgtgt
    10741 aattgggaca ttagccatta caacgagtgg cggaacgatg gtaccagttc aggaattctc
    10801 acctactgag gtgctacaaa ctgttgaaaa agagaaatgt acggcgctac atggcgtacc
    10861 aacgatgttc attagtgaac ttaatttacc gaatttttcg tctttcgatc tatcaacgtt
    10921 gcgtacggga gtaatggctg gctctaactg tcctatcgaa gttatgaaag ctgttatcga
    10981 caaaatgggg atgaaggatg ttacgatctg ctacgggcaa acagaagcct cacctgttat
    11041 tacacaaaca agagctgatg acccactatt gttaaaggta gaaaccgtgg gccgcgcatt
    11101 gccaaatcta gaagttaaaa ttgtcattcc tggtacaaac gaagaagctg cgccgaatga
    11161 acaaggggag ttatgtacga gaggctacca tgtcatgaaa ggttactata aaaacccaga
    11221 agaaacgctt cttgcaatag ataaagataa ctggctgcat acaggggatt tggcaacgat
    11281 ggatgaggcg ggctatgtac gtgtaacagg tcgtttaaaa gatatgatta ttcggggcgg
    11341 tgaaaattta tcgcctagag aaattgaaga attcctttat acacatccaa aaattgcaga
    11401 tgtacaggtt gcgggcgttc ctgatcccgt ttatggagaa gaagcggcag cttggattat
    11461 tttgcgagaa ggagagcagg caacagaaga agagattcga gattattgtc gggataaaat
    11521 ttctagacat aaaataccac gacatatttt ctttatcgat gactatccga tgactgcatc
    11581 aggaaaagtt caaaagtata gattaagaga gagtttcggt gcaacagtga agtaaggagg
    11641 gggcagtgtt atgttcaaga aggttttaat tgcaaatcgt ggagagatcg ctagacgtgt
    11701 tataaggaca tgcaaacgac taaatgttca aacagttgcg gtgtattccg aggcagatgc
    11761 tgaaagctta catgtgaagg acgcggatga ggcgttctgt atcggaaaac cacctgttgc
    11821 acaaagctat ttaaatattg atcgtattct tgaagtggcg aaggaaagcg gtgctgacgc
    11881 tgttcatccc ggctatggct tattatcaga aaacgctgag tttgctaaac gttgtacgga
    11941 ggcgggcctt gtttttattg gaccaagcgc ggacgttata gcttcgatgg gtagtaaatt
    12001 agaagcacgg aaaacaatga aggcagcagg tgtaccaatt gttgaaggtg tagaagcacc
    12061 tttgaaagat gtagcagacg ctatagaaat tgcatcccga ctaggctatc cgattatgtt
    12121 aaaagcctct gctggtggtg ggggtatcgg catgcagctc gttgaaaatg cagaggaatt
    12181 ggcaaaagcc tttgagggca atcaaaaacg tgcacagtca ttctttggtg atggcacgat
    12241 gtatatggaa cgttttattg cgaatcctca tcacgtcgag gtgcaaatta tggcggacca
    12301 tgatggcaat gtcgtcccac tatttgaacg tgagtgctcg attcaacgta gaaatcaaaa
    12361 agtagttgag gaagcaccat ctcctttcat ttctgaagaa acaagaaaac gtatgctaga
    12421 tgcgtcggtc aaggcagtgc aacatattgg ctatgtgaat gccggcacga ttgagtattt
    12481 agtagatgca gagcaaaact tttatttctt agagatgaat acgcgtttac aggtggagca
    12541 tcccgtgact gaggaaatta caaatctgga tcttgtagaa gcgcaattaa agatcgcggc
    12601 aaaacaaccg ctggcattta cgcgcgatag catacaaaga aaaggacatg cgattgaagt
    12661 acgcatttac gcagaaaatc cgtcgacctt tttcccttct ccagggacaa ttactgcttt
    12721 tgaattgcct acaggcgaag gcattcgcca tgaatgtggc gtagaggctg gcactacggt
    12781 aacacctttt tacgatccga tgattagcaa gttggtagtg tggggagaaa cacgaacaat
    12841 tgcctgtgaa agacttattg aggcacttca agcgtataaa gtagaaggaa ttcaaacaaa
    12901 tattccaatg ctactgaaaa cagtcacaca tgaacaattt ttaaaaggct atacaactac
    12961 taaatttgta gatgaatatt atctacctca attagcagag acaaaataaa tacattaaag
    13021 gcattcgcac tttaataacg agtgcgaatg cgttatctaa aacattaatg acacttggag
    13081 ggattgacaa tggcaacagt gaaagcgagt atggcaggga cagtatggaa aatcgttgta
    13141 gcagagggcg agaaagtgac agcaggacaa gatgtagcaa ttttagagtc gatgaaaatg
    13201 gagattccaa ttgcagcaga agaagatggc gtggttacaa aaatcattgc gagcgaaggg
    13261 gattttatta acgttgatga tgatatttta gaaatcgaat aataaggagg tttctttatg
    13321 cagctaccaa agcatgtaac gttaaaggaa gtaggtcccc gtgacggttt gcaaaatgag
    13381 aaaacgcata ttgcaactgc cgataaagta catctagtaa atttacttag tcaaactgga
    13441 ttgaaatata tcgaggtcac ttcttttgta catccgaagt ggattccaca gctggctgat
    13501 gcagtggagg tacttcaagc cattaaacgc caaaaagata tcacatatgc ggcactagta
    13561 ccgaacatgc gtggcttaga acgtgcattg caggctgaag tagatgaagt gagcgtgttt
    13621 atgtcggcca gcgaaagcca taatcaaagt aatattaata aatcgattaa tgaaacattt
    13681 ccgatcttac aggaagttgt ggtaggagca aaggcagcat ataaaaatgt acgaggctat
    13741 atctcaacag tgattgggtg cccgtatgaa ggctacatac atccagaaaa ggttttacga
    13801 gtaacggaga aattattcga aatgggcgtc gatgaaattt cacttggtga cacgattggt
    13861 gttggtgtac cgacacaggt ggaaaatctt ttagaagaat tgctaaaaag atatcctgca
    13921 gaaaatttcg cgatgcattt tcatgataca cgtggtacag cactagcgaa tattgttcaa
    13981 tctttagaaa tgggcattac gaagtttgat agtgcgcttg gtggactcgg aggctgtccg
    14041 tatgccaaag gagcatcagg taatgtagcc acggaggatt tattgtattt attagatgaa
    14101 atgggtatta aaacgggtgt agaacggaat aaagtgctag aggcggcact ctttattgaa
    14161 cagaaactag gaaaggacgt tgcttctaaa caaatggcga ttgtccgtaa tgaaaggagt
    14221 gcgagtcaat tatgacgcaa ctcgttcgtt ttgaggtagt agatcattgc attgggctta
    14281 ttacactttc aagacctgaa gcggcgaatg caatgtctat acaattactc caagaactta
    14341 gcacgacact cgacaacata aatggcgatc cggcagtgag agtcgtctta ctaacgggag
    14401 caggggaaaa agctttttgc gcaggcgcag atttaaaaga gcgtaaaggg atgccagacc
    14461 gacaagtaaa gcagatcgtg caattaatcg gggcaacagt ggcaaaagta gaggcactcg
    14521 ctcaaccagt tatcgccgta ctaaatggtg ttgcttttgg tggtgggctc gaactagcat
    14581 tagcctgtga tttacgtctg gctgctgccc attcaaagat aggtcttacg gaaacatctc
    14641 tcggcatcat cccaggggca ggaggtacac aacgacttcc acgattgatt ggtattggaa
    14701 aagcgaagga attaatttat acagcacgtc gattaaacgc agaggaagca agaagctacg
    14761 gtattgttga gtatgtttat gagggacatg aaatacttga aaaggcacag cagcttgcac
    14821 ttgaaatggc caaaaatgcg ccactttctt tagtacaggc gaaaattgcc atgaatcaag
    14881 gtgttgaagt agatttagct acgggcttaa aaatcgaatc acttgcatat aatgctctga
    14941 ttcctacaga agatcgatta gagggcttac tagctttcca ggaaaagcga acaccgcaat
    15001 atgtagggaa ataaaaggag gcatttaacc aaaaggcaaa gtcatggcta aatcaaaagt
    15061 tgaagataat ctatgccacg attacaagtt aattggagta gagactgggc gactccttgg
    15121 ggatcagcga taggggaacg aaggctaaaa ccatcacatc ctgtgataac gccttcgtga
    15181 ccaacatcgg tgctgctgcc gctacgttgc actcacgctt tcgcgcaaaa aacatctgtt
    15241 ggcccgagac cctggagcga gcaacgcgag tgaagcggct catcggacgc ccccaggaag
    15301 ctctgctctg cgcgaaagcg aagcgtcagc ggcaaagcgc ccggtcggaa cggaaatcaa
    15361 cctcacactt tgtttatgat ctgaaatcta agtgaattta ttataattat ggaatgtaag
    15421 agagaagaaa tacgaagggt aaagtaatta tgctaaggcg taaccgattt gggcaaaagg
    15481 aggaattaac gatgagcaat gtaccgacag aaaatacgac gaaaacaatg aaggaacaaa
    15541 ttttagcagg gggcctgcca aaatatcacg ataaaaatgc acagcaagga aagctatttg
    15601 tacgtgaacg actcgaatta ttactagatg atggactaca atcggaggat ggcttgtatg
    15661 caaactgctt agcaggtgac ttaccagcag atggcgttgt aactggaatc ggtaaaatcc
    15721 atggccgcac tgtttgtgta ttagcgaacg attcgacgat taaagctggc tcttggggta
    15781 aacgtacagt agaaaaaatg attcgtattc aggaaacagc agaaaagcta aattgcccat
    15841 tgttgtatct agtcgattcg gcaggtgcac gtattactga ccaattggaa atgttccctg
    15901 gtcgaagagg tgcaggacgc attttctata atcaggtgaa gctttcaggt aaaattcccc
    15961 aaatatgctt actgtttgga ccttccgcag caggaggtgc atacatccct gcattctgcg
    16021 atattgtagt tatggtagag ggtaatgcat cgatgtattt aggatctcca cgtatggcag
    16081 aaatggttat aggtgagaag gtagatttgg aaacgatggg tggagcgaaa atgcactgtt
    16141 ctgtttccgg ttgcggagat gtgcttgcta aaacagagca agaagcaatt gcctatgctc
    16201 gtaaatattt aagctatttc ccgaacaatt atgcggagcg cagtaaggta gaggctccaa
    16261 aaccaccagc tatatttgat aaatcgattg atgaactaat accgaaaaat caaaacgttc
    16321 cttttgatat gtataagcta atagaacgga ttattgatga agattcgttt tgtgaagtga
    16381 aaaaattatt cgctccagaa ttaattacag gactaggacg cattaatggg caatctgtcg
    16441 gaataatcgc caatcaaccg cgcgtcaagg ggggcgtttt attccacgat tcagctgata
    16501 aagcagcaaa gttcatttca ctttgtgatg cgtttaatat tccgcttctt ttcctcgcag
    16561 atgtaccggg ctttatgatt ggtacgcaag ttgaaaaagc agggattata cgccatggag
    16621 cgaaaatgat ttttgcaatg agcgaggcaa ctgtccctaa attaacggtc attgtccgta
    16681 aggcatatgg tgcagggctg tatgcaatgg caggtcctgc ttttgagcca gactgttgca
    16741 ttgcactatc caatgcgcaa attgctgtaa tgggtcctga agccgcggtc aatgcagttt
    16801 atgccaataa aattgctgaa ctcccaaaag aggagcaagc aagctttatt gcagaaaagc
    16861 gcaaggaata tcaagaggaa atcgatgtct atcgcctagc ttccgagctt atcattgacg
    16921 atgtcattga gccaaatgat ttaagaaaag cactcgaatc acgtttagag ctctatatgt
    16981 caaaatatct attattctcg gaacgtaaac atggagtaaa tccagtataa agcatgaaaa
    17041 aatgctgtct cagttaatgg aggcagcttt tttattgtga gcttttcttt ctaattgaac
    17101 ctggacagac gtatagtata aggggaaaaa ataaaaacgt ttgacctaac ttatttttac
    17161 cccttgcttg gtgaagaggt gcatattaaa atgatgagag gatcaaggga agtaggtggt
    17221 ataggcatgg caaaaaataa tatacagggt agtgccaagc taccgttacc ttatcgttca
    17281 ctgatagata catattattt accgataaag gtaacaggtt ggatattttt ttgcatctat
    17341 tccgtcatag ctttttataa actggtgatt gatcgtctag tgaatgtagt cgtgattttg
    17401 ttccatggcg aatactctct tggtgattta gggctgtttg attacagcaa ctggcaatta
    17461 acgacgaatt ttgttccctt tgatacaatt cttcgttata taaattactc tcagtatttt
    17521 aattttgaca tcattattat taatttgctc ggcaacttat taatatttac tccaatgggc
    17581 tttttactac cgctattatc aaaaaagtgt cgtaaagctt ggaaagttat ttgtataggc
    17641 tttttctcta gtctagcggt agaaaccatc cagtttattt ttagagttgg atcggcagat
    17701 atcgatgatc tgattttaaa taccctggga gcatggcttg gctacctagc ctataaaagt
    17761 atattgatta aaaatagata aaacagtcct tactaaaggg ctgtttttta gtggcgaatt
    17821 aacaccataa actattaaat gaatagaata tcacaagctt atgaaggtct atatgcctta
    17881 tttatcgaaa tttatttatt tctttagaag cttagtattc atttactctt gaatttgatt
    17941 aagaaaattc cgataattag gatacagaaa ctatcatatt tattacgtaa ctttcatatt
    18001 aaattagaaa gccgaggtga atcatatgaa actatcaagt ttaatagaac tagaaagaat
    18061 gtcatcacaa tataatcgta aaaagatttt acagcgaaaa caagcagatg atgcatatcg
    18121 aaaaaactca ttgtatttcc agccaaagaa aaatccgtta ttgcttgagc tcgaaaatct
    18181 acttttaggg cgtacagatc aagatctcta cgagcagcaa aaagaggaat ctaaagaagt
    18241 cacgcctcga cagcaaacaa tcatgcagga tttacaacaa acgaaaaaga atgtgctcgc
    18301 tcatgagcaa acgcaaaaag ctgaagctct tgaatattca cagatagagg atttagatga
    18361 agaagcgcct tttggagatg atgtgaatgc caatgaactg ttccaaacgt tagagaaggt
    18421 aaggactgca gcattagcac ccgcacagcc atcaccacag gatttacgtg ttgcggcaag
    18481 tgcaaatgct caattacttc acatgcaggg gcaactaaat gatcaagagt tagatgaaag
    18541 ggagccttct tttgtacgtg aaactattga agttaaagta cctgaacgtt ttttgaaaga
    18601 attgaaatta gacccatttg cagatacaat tttcggtaag agttatgcag aggcctataa
    18661 ggcgcgaaca tttaaatacg cttctgaaaa atatgcgact catatacaaa tggcgaaaga
    18721 cggctatcaa ccaggaaatg accctaagtt ctctatgata gcctaatgct ggagatggga
    18781 gtttttttaa catggctaaa gcaaagcatg cgaaaacaaa tgcaataaga ttattggagc
    18841 aacaaaaaat acaattcgaa gtaattgaat atgagactgg tgacggtcaa gttgacggaa
    18901 tttcggttgc tgaaaaaatt ggccacccag tttctcgtgt tttcaagaca ttagtagcaa
    18961 aggcaagtgc acaaaaactt tttgtatttg ttattcctgt agcagatgag ctcgatttaa
    19021 aagctgctgc aaaggttgta ggagaaaaga aaatcgacat gctcccagtc aaggaattac
    19081 ttggctatac aggctatgtg cgaggcggat gctctccagt cggcatgaaa aagctttatc
    19141 caactatcat cgatgcatcg gctcaagagc aagaggaaat tattgtgagt gccgggaaaa
    19201 tcggcatgca aatccacgta caactagatg atttagtcgc cgttacaaag gcaaagctcg
    19261 caccaattac cactacccaa gcatgatagg ttgggtagtt ttttattggg cttttaagta
    19321 gtaacagttg aaaaagattg cttggttatg gttatccgct tggcgatgtg gaagaaacgc
    19381 ttaggttact tggcaaactg ttcaggtaag caaagagttc gttcgggtag ctcggtaaat
    19441 tgctcggcta agtcgaataa gccctttata tccgttcaat ctcgatagtc tgaatgcttt
    19501 tcctttacat tgtaaatacg catatgggac ctcctgtaaa taagaaagta tacgttcatg
    19561 attgcatgaa cgtatttttt tattaaaaaa gaacaataat gtgcaattaa ttagttttga
    19621 agagcactta aacaataaaa agaaaaacaa aagtatagtg tagttaaggt aattctcaga
    19681 cggaaagggg gggatgtttt gaaagtgtta attgtttcaa cgtcaacttt atttattgaa
    19741 gcattacaaa tagctattcg tgataggcgt tcaacttggc aagtagatac ttatcaaatt
    19801 caaactccta tatttgatca aaccttgatg aaaaaaatta aagaacaatg ccttgatatt
    19861 gtggtagtgg aagcaactaa tcaacatttt acaatgacaa ttgatagttt gaagaaatta
    19921 aaatgtccag aaatagacat catgttactt gtggattcaa ggatgggtga ggtctaccat
    19981 cacctaaaag atgaagaaag gtggaccagt gttactaaaa atacaagtct ggacgaattt
    20041 ttaatattgt tggaatcatt tggatgtaat atacctgatt taccagatgt ctctttaagg
    20101 gaggtggata aaaagatact aaaagagtta gcggtaggtc actcatttga atttatccaa
    20161 cgtacacaag agctatcggc tgaagaaata gatcagtcac tttatcgaat taatacttat
    20221 tttaaagtac caaattatat agaatccatt gttaaagcat ttgaacagaa aataattaca
    20281 aattgaacat gcgaaggagg aaaattaata tgaaaaaagt tttaggtctt ttaggtggat
    20341 taattattgg catcgttgtt attgctatca taaaaatggt taattaggag agatgcttta
    20401 tgacatttaa aatcgaaaat ttaaataaaa gctttggtga taaaaaagca gtaaacaata
    20461 tttcaattcg attagaagaa gggcaaatat taggcatgct tggtcgaaat ggtgccggaa
    20521 aaacaactac cattcgaatg atgttagagt taattccgaa agatagtgga caaattttat
    20581 ggaaaggaaa acctttctcc aagaagaact taaaaattgg ttatttacca gaagagcgtg
    20641 gtctttatgc gaaaatgaac gttttagatc aaattattta ttttggtatg ttagaaggaa
    20701 tggataaaaa agaagctaaa agagaggccc taaaatggtt agaaaagctt gaaatctctt
    20761 atgcagcaaa gaagcgcact gaacaattat ctaaaggaaa tcagcaaaaa gtacaattaa
    20821 ttacagcaat tatacatgat ccggagttta tcatattaga tgaacctttc agcgggttag
    20881 atccagtcaa tgctgatatg ttaaaaagtg ttgtaaaaga attaatcgct caaaagaaaa
    20941 caattatttt ctgtagtcat caaatggatc aagttgaatc attttgtaac caaatttgta
    21001 ttttgaaaga tggggattta attgtgtcag atcaattatc agctgtaaaa aaatcttatg
    21061 attatcgtta tttagaatta gatacaacag ctaacattaa agattactta gctttgaaac
    21121 aatataaatt cacacaagaa aataatcgtt ataaattgcg tattccacga aatgataaaa
    21181 tccatcaaac gattcgggaa attgatgaaa agtttgattt acaaggagtt tcattgaaag
    21241 aaccaagttt acatgaaatc tttattgaac gaacaggggg ggcgtaaatg cgcaatttta
    21301 gcattgtatt taataacgtt ttcaaagagg ctgtaaaaac aaaggcatat ttatatatga
    21361 cgattggttt agtaggattg agtttacttc tctttgcctc accatctatc atgaaagtct
    21421 ttacttcgaa cagtccggat gagacttata aaattgtcaa tcaaacatct ttagaactat
    21481 ctgaaaaaga gttaacaggg ttagccggaa gctggacaat agtagaaaat gcgaatatag
    21541 aagacctaaa agaagatata aaaactaagg aattaactgg attctttgta ttagaagatc
    21601 aaacgaatgt aatgcaattg aatatctaca tgccgaaagt aaacaatgaa gtgctactta
    21661 cgttagaatc ttatattaaa caaaagaaag tagcgtcaat tgttgctaaa caatcgattg
    21721 acacgggtgt cgttcaagaa ttgaatacac cactccaaag cagtgttcaa tcgttatatg
    21781 aaacagaacc aaatgcgttg tttattattt acggtttaat aactattatg ttcttttcta
    21841 tttcaatata cggtaacaac gttgcaacag cgattgcatc tgaaaaatct tcacgtgtca
    21901 tggaagtcat gattacaaaa gtggcaccag tccctatgat gtacgggaaa attttaggca
    21961 ttggtttagc tagtttaaca caattattta tttttatggg tgccctttta ttatgggcaa
    22021 acgtaggtat ttttgaagtt cctgaaggct cgtttgtaca agctgtaatg gaaactatta
    22081 catggggaac agcagggtat attcttattt tctgtaccat tggatacttt atctatgcaa
    22141 ctttgtatgc aattgttgga tccatgataa gccgcccgga tgatttagca agtgcatcga
    22201 tgccaattat gattatatta atggcgagtt tagtagtaga gatgctcttt gtagtggatt
    22261 ctccagaagg aacgatgacg aatataactt cttacattcc atttacggca ccaacgagtg
    22321 tattgattcg tattgtatat aatacgattt caccaatcga aattgctgtt tcgctattag
    22381 taatgatatc ttttattgct ttatttgcag cagtggcagc gaaaatctat ccaaaaggcg
    22441 ttttaaaatc agatagctta acatttaaac aactaattca atttaataga tagtcctgcg
    22501 attataagaa caaaatgtca agatcttttg ttatcaaagg gcaaaaagaa taattgctct
    22561 agtttatagc aaaacttaaa caaaatagtg aacagcccgt agccacaatt gctggttatg
    22621 ggcgattttt gtacgtggta gcacgcgatg ggatgaactt gtggatgcat ttttatatgg
    22681 agattcttgt agaatggaag gatgaaatag aatttccaga agaaattaaa agtacagaat
    22741 tttttaaaat gaaatataat ggtcttatag taaaatgtta ggagtatgat aaatggatgt
    22801 actaacagga aaaatgctac taatgagcat tattcagttt tctatgatgc tatttttctg
    22861 tacatatatt ttagacttat attggtcaaa aaaacagttg attactatta taattattat
    22921 atttttgcca acttttttgt tgttcccgtt tattggtgcg tggtcaatgt tatattgttt
    22981 atcgctatta gctatcatga tgtatcgaga aacaaaagtt gttatgaaca ttcttcatgt
    23041 gtgtatggca ctgatacttt tagtaatagg ggacaatatt tctaacatac tgacttttcg
    23101 gttgtttaat gggataggga atgagcaact atcaataatt agttatttct ttttttatat
    23161 agtatttggt ctaatatttg ctttttgtta caaaagaatt gtgaaatatt tgataagtcg
    23221 ctgggtaatt aatagctttg tttcgtatat ttcggtattc cttggtatta caacagtcgt
    23281 ttttatgtac atcaatatca tggcaataga ctatgacaat ttctatgaaa gtgttcaaaa
    23341 taatttagcg atgttcctaa tttattttgt attactttca attagtgtat ttgtcgtttt
    23401 atatttagca tttaaacaat ataaaatgaa gcaacgtgag caagaaatga aaaacttcga
    23461 atcctatgtt acatcaattg aacaaattaa ccaagatatg cgcaaattta aacatgacta
    23521 cattaatatt ttatcttcac tgcgtacatt tattgatgat aaaaactatg aaggtctaca
    23581 tacatacttc tatgatcata ttttagaagc gaatcatcat gaacaactaa atcaacaagc
    23641 gatgatgatg ctaaataact taaaaataaa cagtttaaaa gggttagtga ccacgaaaat
    23701 tttacaagcc caatcacatc atgtaccgtt ttatattgaa attgttgagg agattactga
    23761 tattgatgta gatccaattc ttttaaatcg catggtcggc attctactag ataatgggat
    23821 tgaagctgca cgaggagttg aaaatggcga agtacgaatg gcgtttatcc acatgggtga
    23881 ggttgtctta ttagttgtga gtaacacatt tgataagaaa atagctataa aggtacatga
    23941 aatttatcaa gaaggctttt caacaaaggg agaagagcga ggtttaggct tagctaattt
    24001 acgtcaaatg acaaatgatt tacataacgt caatttgaac acgaaaatta gtcctcctta
    24061 ttttattcaa gaagtcgggt ttcgaaaggg atagatagta taaaaaggtg attaaagtgg
    24121 atataacgat tgaatttcta ctaataataa acattattca atattttata atgctctttt
    24181 tctgtaaata tgtattagac ttaaattttt caaaaaaaca atttctattt attat
//
