LOCUS       NZ_JALNLQ010000001     20624 bp    DNA     linear   CON 10-JUN-2024
DEFINITION  Lysinibacillus sp. Bpr_S20 NODE_1_length_919663_cov_121.04, whole
            genome shotgun sequence.
ACCESSION   NZ_JALNLQ010000001
VERSION     NZ_JALNLQ010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Lysinibacillus sp. Bpr_S20
  ORGANISM  Lysinibacillus sp. Bpr_S20
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Lysinibacillus.
REFERENCE   1  (bases 1 to 919663)
  AUTHORS   Longdet,I.Y., Kutshik,R.J. and Okenmor,G.A.
  TITLE     Complete genome sequence of novel Lysinibacillus spp with high
            aromatic hydrocarbon degrading capacity
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 919663)
  AUTHORS   Longdet,I.Y., Kutshik,R.J. and Okenmor,G.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2022) Department of Biochemistry, University of
            Jos, Bauchi Road, Jos, Plateau State 930003, Nigeria
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 11-DEC-2020
            Assembly Method :: SPAdes v. DECEMBER-2020; CheckM's leanage_wf v.
            DECEMBER-2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023337835.1-RS_2024_06_10
            Annotation Date                   :: 06/10/2024 05:11:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,895
            CDSs (total)                      :: 4,826
            Genes (coding)                    :: 4,751
            CDSs (with protein)               :: 4,751
            Genes (RNA)                       :: 69
            rRNAs                             :: 7, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 54 of 75
            Pseudo Genes (internal stop)      :: 13 of 75
            Pseudo Genes (multiple problems)  :: 22 of 75
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:43:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 254710
            Orig. end                         :: 275334
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALNLQ010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20624
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="1"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..20624
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20624
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10624](+)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10624
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    96..111
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 20.96"
                     /tool="antismash"
     misc_feature    175..193
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 23.86"
                     /tool="antismash"
     misc_feature    complement(354..367)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.31"
                     /tool="antismash"
     gene            594..1997
                     /gene="hydA"
                     /locus_tag="MWJ95_RS01260"
                     /old_locus_tag="MWJ95_01275"
     CDS             594..1997
                     /EC_number="3.5.2.2"
                     /GO_function="GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hydA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004232747.1"
                     /locus_tag="MWJ95_RS01260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_01275"
                     /product="dihydropyrimidinase"
                     /protein_id="WP_249074104.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 3.5e-29, bitscore:
                     97.9, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKIIKGGRVATAADVFEADILIENGKIVQIGKDLSEVGAEIIDA
                     TGKIVMPGGIDPHTHLDMPFNNTVTDDDWKSGTIAAAFGGTTTILDFCLTAGEEKLSAA
                     VEKWHDKAKGKSAIDYGFHLMIGDLTHETEAELPQILEQEGITSVKVFMAYAKEFQATD
                     RTLFKAFKIAKDLGAVVMVHCENGSVIDELVEEARRAGHKEPIYHALTRPAELEGEATK
                     RAIELAHIAGATLYVVHVTCKEAVDEIIAAREKGYDVYGETCPPYLTLDQSALAQPGFE
                     GAKYVWSPPLRPKYHQDHLWNALKAKQLQTIGSDQCSFSFKGKKQLGLNDFSKIPNGGP
                     FIEDRFSILYSEGVAKGKISINEFVDMISTSAAKIFGLFPQKGTIAIGSDADIVIFDPE
                     VKREISAKTHHMNVDYNPYEGWEVTGEPVSVLVRGEYVIKNKEFVGVLGSGQYIKRTLK
                     TTAAEALNI"
     misc_feature    complement(2248..2263)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.52"
                     /tool="antismash"
     gene            2363..3730
                     /locus_tag="MWJ95_RS01265"
                     /old_locus_tag="MWJ95_01280"
     CDS             2363..3730
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370857.1"
                     /locus_tag="MWJ95_RS01265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_01280"
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_249103766.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1e-36, bitscore:
                     122.5, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="DAO (E-value: 3.9e-08, bitscore: 29.1,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAPNQENTMSEQLTRNFLELTSRMTKNEAIEESNRCLYCYDAPCI
                     KACPTSIQIPNFIKKIASGNMKGSATTILEANPIGASCARVCPTEELCEGACVLNSSTK
                     PIKIGELQRYVTDWAMATNAQLFKQGQSNGQKVAIIGAGPAGLSAARELSRFGYQVTIY
                     EAESKAGGLGSYGIVSFRLPNEVVDWEIEQIEKLGVEIKTNTTVGVDISAEEILEQYDS
                     VILAVGMGSVPNLGIEGEDLVGVHDAIEFVRKTKMGPLTKDIVGKRVAVIGAGNTAIDG
                     ATCAIRLGADNVQILYRRTQKEMTAYKFEYEFAKQDGVEFKWLTAPKKIIGNESGEVVG
                     IECVKMKLGEVGADGRQRPEEMKGSNFIIEVDAVIKAIGQTRFVSLIEAFGLQHTNGVV
                     DIDETTMQTSNDKVFACGDVVFGNGQGEAMVVTAVQQGKDAAYKIHQRLRKPSEIA"
     gene            3744..5006
                     /gene="preA"
                     /locus_tag="MWJ95_RS01270"
                     /old_locus_tag="MWJ95_01285"
     CDS             3744..5006
                     /EC_number="1.3.1.1"
                     /GO_function="GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA]"
                     /GO_process="GO:0006210 - thymine catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="preA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004232749.1"
                     /locus_tag="MWJ95_RS01270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_01285"
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="WP_249074102.1"
                     /transl_table=11
                     /translation="MADLRIDLAGIKSPNPFWLASAPPTNSGYQVQRAFEAGWGGAVWK
                     TLGDPILNVSSRFAAVSFNGQRVAGFNNIELITDRPLEVNLQEIYETKKRFPDHAIIAS
                     LMVEPKQEKWHEIVKRVEDVGVDGLELNFGCPHGMAERGMGSASGQVPELVEKQTYWVK
                     EAAKTPVIVKLTPNITDITVTAEAATRGGADAISMINTINSLAGVDLDTWNTVPHVAGK
                     GAHGGYCGPAVKPIALNMVAECARNPLINVPISGIGGISNWQDAAEFILMGATGVQVCT
                     AAMHHGFSIVEDMIDGLNNYLDEKGIASVMDLVGRSVSRYSNWGDLDLNYKIVAEINND
                     VCINCNKCHIACEDTSHQCIDLYTEDGRPMLKVREEDCVGCNLCSIVCPVDGAITMVER
                     QSTVPPMTWNERQSLISSLSK"
     gene            5116..6630
                     /locus_tag="MWJ95_RS01275"
                     /old_locus_tag="MWJ95_01290"
     CDS             5116..6630
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004232751.1"
                     /locus_tag="MWJ95_RS01275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_01290"
                     /product="NCS1 family transporter"
                     /protein_id="WP_249103768.1"
                     /transl_table=11
                     /translation="MEREGNYLKSPDLLPVTHQQRNIGTFGFAVIWIGMAIVLAAFAIG
                     GSAIISLPLPMVILATLIGSCLIGVLMTLIGDIGIEHGLSFPVYMRAPFGTFGTHIPSL
                     VRGVTAACWFGLNTYFGALAINGILNLLFSFDNWFICFIVFAALQLFNTSLGIKSIERF
                     ADLAAPVIILISCWMYHTLADTAVAQGKNIWTWVEAPTTGFTAFTAFMVVIMANMGFWA
                     TLAADMPSLSRFFKAPKNERNWFKRNKTQLVGSLIVMPITNTFIVTIGAVCYMAVASAD
                     PINALQQSASGFILGILLLMIVLAQWSTNTSANVIPAATIFSNLGGPKVPFWVGVVIAG
                     IIGILAQPWSLFDVLVNALLIIGGILTAIVGILFADYYLIRKRRVHVKALYELKGQYKY
                     LNGFNLAGLIAWIIGGVMANIFSTYSSLVGFLVGAFVYYVLAKYWWFKKYPQAELEDPS
                     DAKYLGITAGHNLDELFGQQQAPAPIVVPDEDEIAEQPDPLLKGAE"
     gene            6659..6868
                     /locus_tag="MWJ95_RS01280"
                     /old_locus_tag="MWJ95_01295"
     CDS             6659..6868
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004232753.1"
                     /locus_tag="MWJ95_RS01280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_01295"
                     /product="hypothetical protein"
                     /protein_id="WP_249074100.1"
                     /transl_table=11
                     /translation="MSEHQKSTEEIKKVDHYLDSGYKIQYVYENLSGMFVEFARQEEVI
                     LLQILTADARKYFSAKLQEQTLLQ"
     gene            complement(6995..7222)
                     /locus_tag="MWJ95_RS01285"
                     /old_locus_tag="MWJ95_01300"
     CDS             complement(6995..7222)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MWJ95_RS01285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MWJ95_01300"
                     /product="hypothetical protein"
                     /protein_id="WP_249103771.1"
                     /transl_table=11
                     /translation="MTILSVTSDSIRHFGGSIRRFDDSIRHLDSIRRFDDSIRHFGGSI
                     RRLVDSIRHFEGSFRHFGVYPSLRLFYPRF"
     misc_feature    complement(7314..7334)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: medium, score: 26.28"
                     /tool="antismash"
     gene            complement(7320..7517)
                     /locus_tag="MWJ95_RS01290"
                     /old_locus_tag="MWJ95_01305"
     CDS             complement(7320..7517)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MWJ95_RS01290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MWJ95_01305"
                     /product="hypothetical protein"
                     /protein_id="WP_249103773.1"
                     /transl_table=11
                     /translation="MITAKIHHRFGKSIHVFDGSIHVFGGSIRHFDDYIRHFGGSIRRF
                     DDSIRHFDFYPSLRRLYPSL"
     misc_feature    7544..7553
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     gene            7620..8984
                     /locus_tag="MWJ95_RS01295"
                     /old_locus_tag="MWJ95_01310"
     CDS             7620..8984
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1038: phenylalanine-specific permease"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016718131.1"
                     /locus_tag="MWJ95_RS01295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_01310"
                     /product="amino acid permease"
                     /protein_id="WP_249103776.1"
                     /transl_table=11
                     /translation="MANKELKRGLDARHIQMIALGGTIGVGLFMGSASAIQWTGPSVLL
                     AYAIAGLFIFFIMRAMGEMLYMEPSTGSFATFGYKYIHPLAGYLTAWSNWFQWVIVGMS
                     EIIAVGTYMQYWYPDLPAWIPGLIAMVILGVANFISVKTFGEFEFWFAMIKIVTIVLMI
                     IAGFGLIFFGIGNGGVAIGLSNLWNHGGFFTGGWTGFFFALSLVVAAYQGVELIGITAG
                     EAKNPQKTITNAIQSIIWRILIFYIGAIFIIVTVYPWDELGTIGSPFVATFAKVGITAA
                     AGIINFVVITAAMSGCNSGIYSAGRMLYTLAMNGQAPQFFAKLSKNGVPIFGTAGVLIG
                     LFIGVILSYIAPGNLFVYVYSASVLPGMVPWFIILISQLRFRKIKGEQLSKHPFKMPFT
                     PFTNYITIAFLIMVLIGMWINDDTRVSLIVGIIFLVLVIMSYYVFRIGKNRPVNK"
     gene            complement(9065..9829)
                     /gene="pgeF"
                     /locus_tag="MWJ95_RS01300"
                     /old_locus_tag="MWJ95_01315"
     CDS             complement(9065..9829)
                     /codon_start=1
                     /gene="pgeF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381226.1"
                     /locus_tag="MWJ95_RS01300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_01315"
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="WP_249071605.1"
                     /transl_table=11
                     /translation="MKTKIYFDNDQFIAGTTLKDDIELEHNNMALHTCLNEADVLENRK
                     KLATSLDCDLNHFVCTQQTHSANFQKVTISDKGRGAEKMDNAIRNTDALYTVEPNLLLC
                     SFTADCVPVIFYNEVKGLVGVVHSGWQGTVKEVTSKLFQHLIEHEHCNPQDFHVQIGMA
                     LSQQKFEVDADVYDKFHSLGYAEDFIYYNAETNKYHIDNQQTVKKQCELAGIPSKQISI
                     DSTCTFLSPDGFSYRQDKQTGRHLSFIMRKEQ"
     gene            10001..10624
                     /locus_tag="MWJ95_RS01305"
                     /old_locus_tag="MWJ95_01320"
     CDS             10001..10624
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: TS_Pyr4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004232764.1"
                     /locus_tag="MWJ95_RS01305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_01320"
                     /product="hypothetical protein"
                     /protein_id="WP_249103779.1"
                     /sec_met_domain="TS_Pyr4 (E-value: 5.4e-26, bitscore: 87.1,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQEKLLLICHFCMWFFWIITYILIIKQGIKDKRYGMPMAAICAN
                     ISWEFIFSFIHPINDLQRILTFLWFLLDIFILMQFLLYGYKEFKKIIPRKVFYASFFIT
                     LVVSFFTILAMMYEFDDMAGIYAAFFQNLMMSGLFIALFIQRGNLSGQSMGIAVCKMIG
                     TVFAAVGFYLYFRTPLITIISLAIFIYDWIYIVLIYRFSVKFAK"
     gene            10750..11742
                     /locus_tag="MWJ95_RS01310"
                     /old_locus_tag="MWJ95_01325"
     CDS             10750..11742
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009374106.1"
                     /locus_tag="MWJ95_RS01310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_01325"
                     /product="VanW family protein"
                     /protein_id="WP_249103781.1"
                     /transl_table=11
                     /translation="MKKKWMTTLCILCTIGLVGCQGKTADDKKQEQQMQESEQKAEDLK
                     KPVTAEQQPIEPVVVEIIDPSTKVIIQTISPKDMGYERDQQSYTKQIEQLAKKLARGNE
                     KTVGYDKRMILDKLDDHGKIMKGSPLIVLKESELVERILEASATGGKVEMPLIITESGY
                     YLEDIPLLENVVVASYTTYFNTHDVGRNKNIELSAKAINNVIVGSGDYFSFNTVVGPRD
                     EANGYQPAPEIINKKVVMGIGGGICQTSSTLFNAVDQVPVKFVERHHHSLDVGYVPKGR
                     DATVSYGGLDFRFQNTTNVPFLIKANYSKNSLTVEVRTAEKYVEILKRS"
     gene            11850..12674
                     /locus_tag="MWJ95_RS01315"
                     /old_locus_tag="MWJ95_01330"
     CDS             11850..12674
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008405926.1"
                     /locus_tag="MWJ95_RS01315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_01330"
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="WP_249071612.1"
                     /transl_table=11
                     /translation="MHAKLQAAIDSIDFYQVTYPDDACIIVADTEKVIGYKPGKQVDLK
                     IKVGDPVVKYRGTTTEKSLSQGKFIREERGSEGFGLAYIASAQPIFDGGKVIGVLSAII
                     SNEKMDSMRLLATELSSAVEEMTATNEELAVASVDVSNRLGELSEFSGSMTGDIQQINT
                     IVHLVKDLAMKSKILGLNASIEAARSGEHGRGFAVVASEIQKMSQSSTESAESITNQLE
                     NIKHSIDQVNESANQIATFTKQFSVSMYELTDAYKGINSVSEKLMHISEVKA"
     gene            complement(12722..13339)
                     /locus_tag="MWJ95_RS01320"
                     /old_locus_tag="MWJ95_01335"
     CDS             complement(12722..13339)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016995506.1"
                     /locus_tag="MWJ95_RS01320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_01335"
                     /product="HAD-IA family hydrolase"
                     /protein_id="WP_249071614.1"
                     /sec_met_domain="HAD_2 (E-value: 7.5e-36, bitscore: 119.7,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYIIFDFDGTLADSTAVFASAWNTIAQKYKFKGIELKEIEALKK
                     LSIAERSKLFDFPMYKLPMILPQFYRLYRQSLNDVHLYDGMKEVLLEIDKRGYKILIIS
                     SNSQENILEFLKMNGIHCVSDVLCSNRIFGKDKVIKKFLKDSSITSSEVLYIGDEQRDI
                     VACKKAGVPIIWVGWGYDAIEVVQHEEPDYKVAKPQEILTII"
     misc_feature    13520..13534
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 26.4"
                     /tool="antismash"
     gene            complement(13591..14370)
                     /locus_tag="MWJ95_RS01325"
                     /old_locus_tag="MWJ95_01340"
     CDS             complement(13591..14370)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383398.1"
                     /locus_tag="MWJ95_RS01325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_01340"
                     /product="YibE/F family protein"
                     /protein_id="WP_249071616.1"
                     /transl_table=11
                     /translation="MNVLVWLSAILFILMIVIGGKKGVRSFLSLFLNFGVLFITIFLMT
                     NPKNSPVILTLIACTVISWISLFFINEMSSKTMTAFISTMITIVVLLLFIVFVTEKSMI
                     RGFGEESTEEISIFSLYIGVNFAQIGASVIIMSTIGAILDVAISIATSMHEILHHNPAI
                     SRKKLFTSGLSIGKDILGTDTNTLFYAFFGGYLSLLIWFKDLSYSIGEILNSKVFATEI
                     IMIFCAGIGIALIIPITSSVNAYYLTKKREKTENASQ"
     gene            complement(14370..15458)
                     /locus_tag="MWJ95_RS01330"
                     /old_locus_tag="MWJ95_01345"
     CDS             complement(14370..15458)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010676808.1"
                     /locus_tag="MWJ95_RS01330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_01345"
                     /product="YibE/F family protein"
                     /protein_id="WP_249104511.1"
                     /transl_table=11
                     /translation="MIFYTFVVICFIASVYFVYHNEVFYDRSIAEVIKTELKESNPVTD
                     MYENEDRLSTQFITAVLKNGEEKGKLIHFTNEYSASGAYDQEYHVGNKLFVSIKANTDK
                     NGDLTGTILDVKRDQFILIVAWIFIFALLIVGKRQGLFSIISLIVNSLVLSFALDLYVQ
                     SNLNLIFISGVCVLLFTVISLLLINGLNEKTYAAVVATLIGTFISLLISALVLWMTNEN
                     GLRYEEMQFLTRPYRVIFIAGLFIGALGAVMDVSITMTSSMFALFEQNENISDQALKSS
                     GMDIGKDIMGTITSILFFVYICGSIPMLILYLKNSYPLGQTLSLNLSLELARALAGGIG
                     VVLTIPISLYTSIFFINRKRAK"
     regulatory      15889..16015
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     regulatory      16137..16262
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     gene            16404..17042
                     /gene="ribE"
                     /locus_tag="MWJ95_RS01335"
                     /old_locus_tag="MWJ95_01350"
     CDS             16404..17042
                     /EC_number="2.5.1.9"
                     /GO_function="GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ribE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004232779.1"
                     /locus_tag="MWJ95_RS01335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_01350"
                     /product="riboflavin synthase"
                     /protein_id="WP_249103784.1"
                     /transl_table=11
                     /translation="MFTGIVEDIGTVKTLQSDRQSMKITVISKKMVEDVKLGDSIAVNG
                     VCLTVIHFTEQELTMDVMPETVSATNLQQLAIGDPVNLERAMSANGRFGGHFVSGHVDG
                     VGKVVRKRPLANAVYIDIELSKELTSFCIPKGSITIDGTSLTLFHVENNSVTVSLIPHT
                     YKETILGKKKIGELVNVETDLVGKYILHQLKSGQEPPTITRDFLAQNGF"
     gene            17151..>17744
                     /gene="ribB"
                     /locus_tag="MWJ95_RS01340"
                     /old_locus_tag="MWJ95_01355"
                     /pseudo=""
     CDS             17151..>17744
                     /GO_function="GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ribB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016096152.1"
                     /locus_tag="MWJ95_RS01340"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_01355"
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLHSIEEAIEDLKQGKIIIVVDDEDRENEGDLLALAEFMTPETIN
                     FMATYGRGLICTPISQQLAQKLALNPMVSMNTDHHQTAFTVSIDHVNTSTGISAFERAM
                     TIQKMIEPQAKAADFHRPGHVFPLIAKDNGVLERRGHTEATIDLAKLCNSIPAGIICEI
                     MGDNGKMLRFNDLVKYAAQHQLKMISIEALVTYRR"
     gene            17976..18443
                     /gene="ribE"
                     /locus_tag="MWJ95_RS01345"
                     /old_locus_tag="MWJ95_01360"
     CDS             17976..18443
                     /EC_number="2.5.1.78"
                     /GO_component="GO:0009349 - riboflavin synthase complex
                     [Evidence IEA]"
                     /GO_function="GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="ribE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016995511.1"
                     /locus_tag="MWJ95_RS01345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_01360"
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="WP_249071619.1"
                     /transl_table=11
                     /translation="MGNTFEAQLIGTDLKIAVVVGRFNEFITSKLLDGALDGLKRHGVD
                     ENAIDVAWVPGAFEVPFIAKQLAETKKYDAIIGLGTVIRGSTTHYDYVCNESAKGIANV
                     SLTTNVPVIFGIVTTENIEQAIERAGTKSGNKGYDAAMSAIEMANLKKMIG"
     gene            18591..20045
                     /locus_tag="MWJ95_RS01350"
                     /old_locus_tag="MWJ95_01365"
     CDS             18591..20045
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004232782.1"
                     /locus_tag="MWJ95_RS01350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWJ95_01365"
                     /product="spore germination protein"
                     /protein_id="WP_249071621.1"
                     /transl_table=11
                     /translation="MEQELNLKSLKSLFQRSADVIFQEYFFQQHKVHLIKCEAMIDERL
                     LQTVIVQRVQSLFTEASDELLEEHIQTNLHIPHLQRITSKNDAMTRVYKGHVLLYFEHL
                     NLLYTSYIAKKPNREPKETTLELVVRGPRDDFIEDVFINIALIRKRVPTNSLSVETYEL
                     GKRSKTTVAVLYFEDVVNQKMLEKIKSQLQRIDADIVFSGDLLMERIEQSAKILPRHDY
                     TGRPDYAVQALSRGRVIIIVDGVSYAVITPANTMLLFKSGEDNEYPIIVSSMERILRII
                     AILISILLPGFWLALSTYHQNQLPFILLATVVESRTGLPFPTILETLMMLFMFEIFREA
                     NIHLPNVISGSISVVGGLIIGDAAIKAGVTSPSMIVVIATSSIAAFTLGNQSIVSAMSI
                     LRVIIILASAFFGLFGFFVSVFFILLYTANIRVLGVPYLNMGADLNWQDIKKSLVRLSP
                     SGYRMRPKFLNVQDETKTSSKKKINT"
ORIGIN
        1 caaaaatcat ttcgtattta catgaagagc tcggaaaaat tgatgaacag ctagtgttta
       61 cctttggtcg aatgaatgta tctccaaata ttcatacggt tataccgaac aatgtgactt
      121 ttacgataga ttcacgtcat caaaaaccag agattatgca aaaggttgag gacatactga
      181 ataatctccc agcagaggat atgggctgta gcattaagcc agttaaacta tggggcagag
      241 atacagtcca ttttgatact caaatttgta atgaagtgga aaatgcttgt cagagctttg
      301 gctatactgc tcatcgaatg tttagcgggg cagggcatga cgcgcaatat atggcaagca
      361 ttgtgccatc agcgatgatt ttcgtaccaa gtattaatgg gaaaagtcat tgcgaggaag
      421 aggaaacaac atttgaggat tgcgcgaaag gagcagatgt gttgcttgaa acagtattga
      481 cattacaaac aaagttcagt atgggagaga cttacacgtt gcattaaggt aagattgaca
      541 aaaattatca cgattgagca ttctccatta ttttaagaag ggtgaggggc agaatgaaaa
      601 aaattattaa aggtggacga gtagcgacag ctgcagatgt ttttgaggca gatattttaa
      661 ttgaaaacgg aaaaattgtt caaattggca aagatttatc tgaggtgggc gcagaaatta
      721 ttgatgctac tggtaagatt gtcatgccag gtgggattga tccgcacaca catttagata
      781 tgccatttaa caatacggta accgatgatg attggaaatc tggtactatt gcagcggctt
      841 tcggtggaac aactactatt ttggacttct gcttaacggc tggcgaagaa aaactttcag
      901 ctgccgttga aaagtggcat gacaaggcga aggggaaatc agcgatcgat tatggattcc
      961 acttaatgat tggggattta acgcatgaaa cagaagctga attgccacaa atcttagaac
     1021 aagagggcat tacttctgta aaggtattta tggcttatgc gaaagaattt caagcaactg
     1081 accgcaccct ttttaaagct tttaaaatag cgaaagacct cggtgctgtt gttatggtgc
     1141 actgtgaaaa tggttctgtt attgatgagc ttgtggaaga agcgcgacgt gcaggtcata
     1201 aagagccgat ttatcatgcg cttactcgac cggctgaatt agaaggagaa gcgacaaaac
     1261 gggcgatcga gttagcccat attgctggag ctactcttta tgttgtgcat gtcacttgta
     1321 aagaggcggt agatgagatc attgccgcac gtgaaaaagg ctatgacgtc tatggtgaaa
     1381 catgtccacc atatttaact ttggatcaat ctgctttagc acaaccgggt tttgagggtg
     1441 caaaatatgt ttggtcccca ccacttcgcc ctaaatacca tcaggatcat ttatggaatg
     1501 ctttaaaagc aaaacaacta caaacaatcg gatctgatca gtgctcattt agttttaagg
     1561 ggaaaaagca gttaggatta aatgatttct ctaaaattcc taatggtggg ccttttatcg
     1621 aagatcgctt cagtatttta tattcagaag gcgtagcaaa aggaaaaatt tctatcaatg
     1681 agtttgtcga tatgatctcc acaagtgcag ccaaaatatt tggcttattc ccacaaaaag
     1741 gaacgattgc aattggttct gatgccgata tcgttatctt tgatccagag gtaaaacgag
     1801 aaatttctgc taaaacacat catatgaacg tagattataa tccgtatgag ggatgggaag
     1861 taactgggga gccagtaagt gtacttgtac gtggagaata tgtcattaaa aataaagaat
     1921 ttgttggggt attgggtagt gggcaatata tcaaacgtac attaaaaact acggctgcag
     1981 aagcactaaa catttagcac aggctgtaat actcccatga aagtacgact tgttcaatta
     2041 ataatcagtg agggatgaag aaaaaaccac tgattaaagt ttcactttta accgattgct
     2101 ttgaatggag aacagagcaa tcggtttcat aatatcttaa ttcagctaca gaagataatc
     2161 taccgtgaaa tgaacgttta atgtagctct ttttcataat taataaggct catcactatg
     2221 tatataggtt gattggcgtg aaggcctgcg actcctttgt gaccaacctc gagttggccc
     2281 gacatgcgcc cagcaggagc ggaaatcaac cacaagtgat ggtgacgagt catgaaaaaa
     2341 tgaacatgag ggggatgtgg aaatggctcc gaatcaagaa aatacaatgt ccgaacagtt
     2401 aacacgtaat tttttagagt tgactagtcg gatgacgaag aatgaagcaa ttgaggaatc
     2461 caatcgttgt ctttattgtt acgatgcacc gtgtattaaa gcttgtccaa cgagtattca
     2521 aatcccaaat ttcataaaaa aaattgcttc tggtaatatg aagggctctg ctacgactat
     2581 attagaagca aatccaattg gtgcaagctg tgcaagggtt tgtccaacgg aggaactatg
     2641 tgaaggggcg tgtgtgttaa attcctcaac aaagccaatt aaaatcggag agttacagcg
     2701 ctatgtaaca gattgggcaa tggcaacgaa cgctcagcta tttaagcaag ggcaaagcaa
     2761 tggacagaag gtagcgataa ttggcgcagg tcctgctggt ttatccgctg cacgtgaatt
     2821 aagtcgattt ggttatcagg tcacgattta tgaagctgaa tcgaaagcgg gtggcttagg
     2881 gagctatggt attgtgtcat tccgtttacc gaatgaagtc gttgattggg aaattgaaca
     2941 gatcgaaaag cttggtgtgg aaatcaaaac aaacacaact gtgggtgttg atatttctgc
     3001 tgaagaaatt ttggagcaat atgacagtgt tattttagca gtcggcatgg gatctgtgcc
     3061 aaatttaggc attgaaggtg aagatttagt cggtgtgcat gatgcaatag agtttgtcag
     3121 aaagacaaaa atgggaccgc ttacaaaaga tattgttggg aagcgagtgg ctgtcattgg
     3181 tgcaggaaat accgcgattg atggtgctac atgtgcaatc cgtttaggtg ctgataatgt
     3241 tcaaattctt tatagaagaa cgcaaaaaga aatgactgcg tacaaatttg aatatgaatt
     3301 tgccaagcag gatggtgtgg aatttaagtg gctaacagca ccgaaaaaaa ttatcggtaa
     3361 tgagtcaggt gaggtcgtag gtatagaatg tgtgaaaatg aagctcggtg aagtaggtgc
     3421 tgatggccga caaagacctg aggaaatgaa gggatctaat tttattattg aagtggatgc
     3481 tgttattaag gcaatcgggc aaacacgttt tgtgtcatta attgaagctt ttggcttaca
     3541 gcatacaaat ggcgtggtcg atattgatga aacgacaatg caaacttcaa atgacaaagt
     3601 atttgcatgt ggagatgtcg tcttcggaaa tggccagggc gaggcgatgg ttgtcacagc
     3661 tgtacagcaa ggaaaagacg cagcatacaa gatccaccaa cgtttaagaa aacctagtga
     3721 gattgcataa gggaggaact actatggcag acttacgcat tgacttagcg ggcatcaaat
     3781 cgccgaatcc attttggctt gcatcagcac caccgacgaa ttcaggttat caagtacaac
     3841 gcgcatttga agcagggtgg ggtggtgctg tgtggaaaac attgggggat ccgattttaa
     3901 atgtttcttc acgctttgca gctgttagct ttaacggcca aagagtagct ggcttcaata
     3961 atattgaatt aattacggat cgtccattag aggtaaattt acaagaaatt tatgaaacga
     4021 aaaaaaggtt tccagatcac gcaatcatcg cttccttaat ggtggagcca aagcaagaaa
     4081 agtggcatga gattgttaaa cgagtagaag atgtgggggt tgatggtctt gagctgaact
     4141 ttggttgtcc acatgggatg gcagaacgag gaatgggctc tgcctctggt caagtacctg
     4201 aattggtgga aaagcaaacg tactgggtga aagaggcagc gaaaacacct gtaatcgtga
     4261 agttaacacc aaacattacc gacattacgg taacagcaga agcagccaca agaggtggag
     4321 cagatgccat tagtatgatt aatacaataa acagcttagc cggtgtcgac ttagatacat
     4381 ggaatacagt acctcatgta gcaggaaagg gtgcacatgg tggctattgt ggtccagccg
     4441 tgaaaccaat tgcactcaat atggttgcag aatgtgctcg taatccatta atcaatgttc
     4501 caatctcggg tattggtggt atttccaact ggcaagatgc ggcagagttt atattaatgg
     4561 gcgctactgg cgtgcaagtt tgtacagcgg cgatgcatca tggctttagt attgttgagg
     4621 atatgatcga tggactcaat aactatttag atgaaaaagg tattgcctct gtcatggatt
     4681 tagtaggtcg ttctgtttcc agatattcta attggggcga cttggattta aattataaga
     4741 tcgtggctga aattaataac gatgtttgca ttaactgtaa caaatgccat attgcatgtg
     4801 aggatacatc ccatcaatgt attgatttat atacagaaga tggtcgccca atgttgaaag
     4861 ttcgagaaga ggactgtgta ggctgtaatt tatgttcaat tgtttgtcca gtagatggtg
     4921 ctattacaat ggttgaacgt caatctacag tgccaccaat gacttggaat gaacgtcaat
     4981 ctctgattag tagtctatct aagtaaacaa aacttttaaa taaactgaaa ttccaccttc
     5041 cttcagcatt ttcgttgaag ggaggaatga ccccatgtaa gttgtcacga aaattggaaa
     5101 aaaaggagag attatatgga acgtgaaggg aattatttaa aatccccaga tttacttcca
     5161 gtgacacatc aacaaagaaa tattggaaca tttggttttg cagtaatttg gataggtatg
     5221 gcaattgtat tagcggcttt tgcaatcggt ggctcagcca tcataagctt accattgcca
     5281 atggtaattt tagcaacgtt gattggctcg tgtttaattg gtgtgttgat gacgttaatt
     5341 ggtgatatag gcattgaaca tgggttatca ttccccgtct acatgcgtgc tccgttcggt
     5401 acatttggca cacatattcc gtcacttgtt cgtggtgtaa cggctgcctg ttggtttggt
     5461 ttaaacacgt attttggagc attagctata aacggtattt taaatttact ttttagtttt
     5521 gataattggt ttatctgttt cattgtcttt gcagcacttc agttatttaa tacatcactc
     5581 gggattaaat ctattgaacg ctttgcagat ttggcagcgc cagttattat tttaatttca
     5641 tgctggatgt atcatacatt ggcagatact gcggtagctc aagggaagaa tatttggacg
     5701 tgggtggaag caccaacaac aggatttaca gccttcactg catttatggt agtgattatg
     5761 gcgaatatgg gattttgggc gacgctagca gccgatatgc cctcactttc tcgcttcttt
     5821 aaagcaccaa aaaatgaacg aaattggttc aaacgcaaca aaacacaact tgttggctca
     5881 ttaatcgtca tgccgattac aaatacgttc attgtaacaa ttggtgctgt gtgctatatg
     5941 gccgttgcct ctgcagatcc tatcaatgca ttacagcaaa gtgcaagtgg ctttatttta
     6001 gggattttac tattaatgat tgtattagct caatggtcaa ctaacacctc tgcaaatgtg
     6061 atcccagcgg caacaatttt ctcaaatctc ggtggtccga aagtgccgtt ttgggttggg
     6121 gttgttattg caggaatcat tggtattcta gcacagcctt ggagcttatt tgatgtgtta
     6181 gtcaatgcac ttttgattat cgggggtatt ttaacagcaa ttgttgggat attatttgct
     6241 gattattatt taattcgtaa acgtcgtgtg cacgtaaaag cactttatga attaaagggt
     6301 cagtataagt atttgaatgg ctttaactta gccggcttaa tcgcttggat tatcggtgga
     6361 gtaatggcaa atatattttc cacgtattct tcattagtag gattcttggt gggggcattt
     6421 gtatattatg tactagcgaa gtattggtgg ttcaaaaaat accctcaagc tgagctagaa
     6481 gatccaagtg atgcaaaata tttgggaatt acagctggac acaatttgga tgaactattt
     6541 ggacagcaac aagcgcctgc tccaattgtt gtccccgatg aagatgaaat cgctgaacaa
     6601 ccagatccgc tgttaaaagg tgcagaataa aaattaaaca ccaaggtgag gtgattgcat
     6661 gtcagaacat caaaaaagca cagaggaaat caaaaaagtg gatcattatt tagatagcgg
     6721 ctataaaatc caatatgttt atgaaaattt aagtggaatg tttgtagaat ttgcccgtca
     6781 agaagaagtg atcctgctac aaattctaac agcagatgca cgcaaatatt tctccgcaaa
     6841 gcttcaagaa cagacgttat tgcaataaca aaggtaactc tatttcattt aatgaaatag
     6901 agttttttta tggaatatgt tgctgataac ggctatccaa ctgtaatgac agccaaagat
     6961 gtggatagag tcgtcaaagt gacgtataga acggtcaaaa tcgtggatag aacagtcgaa
     7021 gtgatggata gactccaaag tgacggaaag aaccctcaaa gtgacggata gaatcgacta
     7081 agcgacggat ggaaccaccg aagtgacgga tagaatcgtc aaagcgacgg atagaatcca
     7141 agtgacggat agaatcgtca aagcgacgga tagagccgcc gaagtgacgg atagaatccg
     7201 aagtgacgga tagaatcgtc aaagcgacgg atggaaccac cgaagtgacg gatagaaatc
     7261 aaagtgacgg atagaatcgt caaagcgacg gatagaatcc gaagtgacgg atataatcgt
     7321 caaagtgacg gatagagccg ccgaagtgac ggatagaaat caaagtgacg gatagaatcg
     7381 tcaaagcgac ggatggaacc accgaagtga cggatataat cgtcaaagtg acggatagaa
     7441 ccgccaaaaa cgtggataga gccgtcaaaa acgtggatag acttgccgaa gcggtgatgg
     7501 atttttgccg tgatcatttt tgtttttcct aaaaatacaa ttgtgttttt tgatagaatt
     7561 gattattatt ataaaataat tcatcgtaga agaacttcag agaaagaggc gtagtttgag
     7621 tggcaaataa agaattaaaa aggggattag atgcacgtca tatccaaatg attgctcttg
     7681 gaggcactat tggcgtgggc ttgtttatgg ggtctgcaag tgctattcag tggacaggac
     7741 cttccgtgct ccttgcatat gctatagcag gtctatttat ttttttcatt atgcgagcaa
     7801 tgggagaaat gctttatatg gagccgagta cgggttcctt tgcaacattt ggctataaat
     7861 atatacaccc attagcaggt tatttaacag cgtggagtaa ctggtttcag tgggttattg
     7921 ttgggatgtc agagattata gcagtaggca cgtatatgca gtattggtat cctgatttac
     7981 cagcatggat tccaggattg atcgccatgg tgattttagg tgtggccaac tttatttctg
     8041 ttaaaacttt tggagaattt gagttttggt ttgctatgat taaaatagta acgattgttt
     8101 taatgattat tgcaggcttt ggtcttattt tcttcgggat tggtaatggg ggcgtggcaa
     8161 tcggattatc gaatctttgg aatcatggag gtttctttac tggcggctgg acaggtttct
     8221 tctttgcgtt atcattagta gttgcagcct atcaaggtgt tgagctcatt ggaattacag
     8281 caggagaagc gaaaaatcct caaaaaacta taacgaatgc gattcaaagt attatttggc
     8341 gtattttaat attttatatc ggtgcaatct ttatcattgt aacggtttat ccgtgggatg
     8401 agttaggcac gattggtagc ccgtttgttg caacttttgc aaaggttgga attaccgctg
     8461 cggcaggtat tattaatttt gtggtcatta cagcagcaat gtcaggctgt aatagtggaa
     8521 tttatagtgc tggacgtatg ctatatacac ttgccatgaa tggacaagca ccacaatttt
     8581 ttgcgaagct ttctaaaaat ggtgttccga tatttggcac tgcaggcgtg ctaatcggat
     8641 tgtttatcgg cgttatttta agttatattg caccaggaaa tttatttgtc tatgtataca
     8701 gtgctagtgt tttaccgggg atggttccgt ggtttattat tttaatcagt caactacgat
     8761 ttagaaaaat taaaggtgaa caactttcca agcacccatt caaaatgccc tttacaccat
     8821 ttacaaacta tataacgatt gcctttttaa taatggtgct aatcggtatg tggattaatg
     8881 atgatactag agtttcactt attgtgggca ttattttctt agttcttgtg atcatgagct
     8941 attatgtttt tagaattgga aaaaatcgtc ccgttaataa ataatgaaat gtaaaaagga
     9001 tgtatttctc tactatgaga tacatccttt ttgcttatta tcaatagtgg attgtccctt
     9061 ttaactactg ttctttgcgc ataataaagc ttaaatgtct accagtctgt ttatcttggc
     9121 ggtaggaaaa accatctggg ctaagaaatg tgcacgttga atcaatcgaa atttgcttag
     9181 aaggaatacc tgcaagctca cattgttttt tgaccgtttg ttgattatcg atatgatatt
     9241 tatttgtttc tgcattgtaa tatataaaat cctccgcata gcctagacta tggaatttat
     9301 cgtaaacatc tgcatctacc tcgaatttct gctgactgag tgccataccg atttgtacat
     9361 gaaaatcttg cggattacaa tgctcatgct ctatcaaatg ctggaaaagc tttgatgtta
     9421 cttccttgac agtgccttgc cagcctgaat gaaccacgcc aacaaggccc ttgacctcat
     9481 tataaaaaat gacagggacg caatcggctg taaaactaca taaaagtaaa ttaggctcta
     9541 ctgtataaag tgcatcggta ttcctaatgg cattgtccat cttttcagcg ccacgtcctt
     9601 tatccgaaat cgtgaccttt tggaaattag cactatgcgt ttgttgcgtg catacaaaat
     9661 gatttaagtc gcaatctaaa gaagttgcta atttttttcg attttctagg acatcagctt
     9721 catttaaaca agtatgcagt gccatattgt tatgttctag ctcgatgtca tcctttaagg
     9781 tagtgcctgc gataaattgg tcattatcaa aataaatttt agttttcata tcatcacctc
     9841 gtcttactat atatgattgg tccatgaatg tataggcgct tacgcttagg attttaagag
     9901 ctttcagtaa ttacatatcc ttctacaatt tttttagaca taaagaatag atttcttcac
     9961 atatagtatc gaagaatcat aaatgaagga aggtgtaaaa atgactcaag aaaaacttct
    10021 attaatctgc catttttgta tgtggttctt ttggattatt acatatatat taatcattaa
    10081 acaaggcata aaggataaga gatatggaat gcctatggcc gcaatttgtg ccaatatttc
    10141 atgggaattt attttttcat ttattcaccc aataaatgac ttgcaaagga ttcttacttt
    10201 cttgtggttt cttttggata tcttcattct gatgcaattt ttactttatg gttataagga
    10261 atttaaaaaa attattccga gaaaggtatt ttatgcatct ttttttataa cactcgtagt
    10321 aagctttttt acgattttag cgatgatgta tgaattcgat gatatggcag gcatatacgc
    10381 cgcatttttc caaaatctga tgatgtcagg gctttttatc gccttattca tacaaagagg
    10441 aaatttaagt ggtcaatcaa tgggcatcgc cgtttgtaaa atgattggaa ctgtgtttgc
    10501 tgcagttggc ttctatttgt attttagaac accattaatt actattattt cgctggcaat
    10561 ctttatttat gattggattt atatcgtttt aatctatagg ttttctgtga agtttgctaa
    10621 atgaaaaaac ggataaagat gtaccgtgac cttctttttt taaacaaata aaaatgatga
    10681 aatgaaacac actatcagca atacacgaat aagatgtctc tgtagtatga agataggtag
    10741 gtgttttatt tgaaaaagaa gtggatgacc acattatgta ttctttgtac aattgggcta
    10801 gttggctgcc aggggaaaac tgcggatgac aaaaagcaag aacaacaaat gcaagagtca
    10861 gagcaaaaag ctgaggattt aaaaaagcca gttactgcag aacaacaacc aatagagcct
    10921 gtcgttgttg aaattataga tccttcaacg aaagtcatca tacaaacaat ttcaccgaaa
    10981 gacatgggct atgagcgtga tcaacaatcg tatacgaaac aaattgagca attagcgaaa
    11041 aagttggcga gagggaatga aaaaacggtt ggctatgata aacgtatgat cctcgataag
    11101 ctggacgatc atgggaaaat aatgaaaggt agtccgttaa ttgttttaaa agaaagtgaa
    11161 ttagtagaaa gaatattaga agcatcggct accgggggta aagtggagat gccccttatt
    11221 attacagaaa gtggctatta tttagaagat attcctttgt tagaaaatgt agtagtggca
    11281 tcctatacaa catacttcaa tacccatgat gttggtagaa ataaaaatat cgagctttct
    11341 gcgaaggcca tcaataatgt tatcgttgga agtggagatt atttctcgtt taatacagtc
    11401 gttggaccta gagatgaagc gaatggatat cagcccgcac cagaaattat taacaaaaag
    11461 gttgtgatgg gcattggggg aggtatttgt caaacgtctt caactttatt taacgcagtt
    11521 gatcaagtac ctgtaaaatt tgttgaacgg catcatcatt ctttagatgt tgggtatgtt
    11581 ccaaaaggga gagacgctac tgtttcttat ggtggtttag attttagatt tcaaaataca
    11641 actaacgtac cgtttttgat caaagcaaac tatagtaaaa attcgctaac agttgaggta
    11701 agaactgcag agaaatatgt agaaatcttg aaaaggtcgt agtagttttc caaagacact
    11761 ttagcttaag gtgtcttttt taaattgtca tgctatagtt aaattgaggg attttagtat
    11821 aatcgtgtta tgggagaagg ggaaaaataa tgcatgcaaa actccaggca gcaatagatt
    11881 ctatcgattt ttaccaagtt acatatccag atgatgcttg tattatagtg gctgatactg
    11941 agaaggtcat cggctacaag ccaggtaaac aagtagactt aaagattaaa gttggggatc
    12001 ctgtagtcaa gtatcgtgga acaacaactg aaaagtctct aagccaagga aagttcattc
    12061 gggaagagcg aggttctgag ggctttggac tggcgtatat tgcctctgca cagcctattt
    12121 ttgatggtgg aaaagtaatc ggtgtgttaa gtgccattat ttcgaatgaa aaaatggata
    12181 gtatgcgttt attagcaact gagctttcga gtgctgttga agaaatgacc gcaacgaatg
    12241 aagagttagc agtggcaagt gttgatgtat ccaatcgctt gggagagcta tccgagtttt
    12301 cgggatcaat gactggcgat attcaacaaa tcaatacgat tgttcattta gtaaaagatt
    12361 tagctatgaa atctaaaatt cttggattaa atgcatctat cgaggctgct cgctcaggtg
    12421 agcatgggag aggctttgca gtagtcgctt ctgaaataca gaaaatgtca caaagtagta
    12481 cggaaagtgc cgaaagcatt acaaaccaat tagaaaacat taaacattct attgatcaag
    12541 tcaatgaatc agcaaatcaa atcgcaacgt ttaccaagca attttcagtg agtatgtatg
    12601 aattaaccga tgcttataaa ggaattaaca gcgtatcaga aaaattaatg catattagcg
    12661 aagtaaaggc ttaatcatta taaaaggata atagcaaaat attcgctatt atccttttcg
    12721 tctatatgat agttaaaatt tcttgcggtt tagcaacttt gtaatcgggt tcctcatgtt
    12781 gtacaacttc aatagcgtca tatccccaac caacccaaat gataggcaca cccgcttttt
    12841 tgcaggctac gatatctctt tgctcatcac caatgtataa aacttctgac gatgtaatgc
    12901 ttgagtcctt taaaaatttc tttatcactt tatcctttcc aaaaatacgg tttgaacaaa
    12961 gaacgtctga tacacaatgg atgccgttca tttttagaaa ttctaaaatg ttctcctgtg
    13021 aattggagga aataataagg atcttataac cccttttatc aatttccaac aggacttcct
    13081 tcataccatc ataaagatga acatcattta atgactggcg atacaagcga taaaactgag
    13141 gtaaaatcat gggcaattta tacataggga agtcgaataa tttacttcgt tccgctatag
    13201 ataatttttt caatgcttca atttccttta actcgattcc tttaaattta tatttttgcg
    13261 cgattgtatt ccaagccgac gcaaacactg ccgtagaatc tgccaatgtt ccatcaaaat
    13321 caaagataat atatttcatt acaaattttc tcctttatca tctaattgta aagacgtttc
    13381 tttcttgaaa atgttactgc ctatactaaa aaaagtgaca tgattataca ggatatgaaa
    13441 caaagtatta caaagaactc taaatgagca atattttttt ctaaataaat ttttctaatg
    13501 taaagccttc tgattgctga attttcagat aattttatta tgtaaaattc ctgtcattcg
    13561 ttcgcatagc aaacgagtga caggtacagt ctattgtgaa gcattttctg ttttttctcg
    13621 tttttttgtt aaataatagg catttaccga agatgtaata ggaatgatca gtgcgatacc
    13681 aatccctgca caaaaaatca taataatttc agtggcaaat actttagaat ttaaaatttc
    13741 accaattgaa taagataaat ctttaaacca aatgagtagg cttagatatc caccaaagaa
    13801 agcatagaat aaagtatttg tatctgtacc taaaatgtcc tttccgatgc ttaaacctga
    13861 tgtaaatagt ttttttctac ttattgcagg attatggtga agaatctcat gcatggaagt
    13921 ggcaatggaa atggcaacat ccaaaattgc accaatggta ctcataataa tgacggaggc
    13981 tccaatttgc gcaaagttaa cccctatata aagtgaaaat atgctaattt cttctgttga
    14041 ttcttcccca aatcctcgaa tcattgattt ttccgtaaca aaaacaatga aaagtaataa
    14101 aactacaatc gtaatcattg tagaaatgaa agcagtcatc gttttactac tcatttcatt
    14161 tataaaaaat aagctaatcc aacttatgac ggtacaggca ataagcgtta atataaccgg
    14221 actattctta ggattggtca ttaaaaagat tgtaataaag agtaccccaa agttcaaaaa
    14281 taaagataga aaagaccgta ctcctttttt tccaccaata acgatcatca atatgaataa
    14341 aatggcactt aaccaaacta atacattcat tattttgccc tctttcgatt aatgaaaaaa
    14401 atagaagtat atagactgat cggaatcgtt aaaacaacac caattccacc tgctagtgca
    14461 cgagctaatt ctaacgaaag gtttaaggaa agtgtttgtc ctaatggata agaatttttt
    14521 aaatataaaa ttaacatagg aattgaacca catatataaa caaagaacaa aatacttgta
    14581 attgttccca taatatcttt cccgatatcc attcctgagc ttttcagtgc ttggtcagag
    14641 atattttcat tttgttcaaa taaagcgaac attgaggaag tcattgtaat ggagacatcc
    14701 attacagctc ctagtgcccc aataaatagc cctgctataa aaatcactcg ataaggtcgc
    14761 gttaaaaatt gcatttcttc atatcgtagc ccattctcgt tagtcatcca cagaactaaa
    14821 gcgctaatca ataacgaaat aaatgtccct ataagtgttg caacaaccgc tgcatatgtt
    14881 ttttcattca agccgttaat aagtagtaag gatatgacgg taaataaaag aacacatacc
    14941 ccgcttataa atatcaaatt taaatttgat tgtacatata aatccaatgc gaaggataat
    15001 acaagagagt ttactattaa actgatgatt gaaaataaac cttgtctttt accaacaatc
    15061 aacaacgcaa aaataaaaat ccacgctact attaagataa actgatcacg ttttacatct
    15121 aaaatagtcc ccgttaaatc tccatttttg tctgtatttg ctttaatgga aacaaataat
    15181 ttattcccaa catggtactc ttgatcgtag gcaccagaag ctgaatactc atttgtaaaa
    15241 tgaataagct tccctttttc ttccccattt tttaaaacag ctgttataaa ctgagtagat
    15301 aaacgatctt cattttcata catatctgtt acagggtttg attcttttaa ttccgttttt
    15361 ataacttctg caattgatcg atcataaaat acttcgttgt gataaacgaa ataaactgaa
    15421 gcgataaagc aaattacaac aaaagtataa aatataatgt tgtaataaga tttttttttc
    15481 aagatgtttt gcaatataaa acctccgatg agaaaaggga acctaatcat gttgcgttat
    15541 agtcatagtt agtgctgaca cgaacaatga ctcacaattt ttactctgat tttttggtca
    15601 agcagataaa aaagtgccat tataaagtaa cgtgctaatt gaatagaggt tccaaaataa
    15661 ttttagtcat ggctaatggc atactaagtt agttatagca taattaaaat gtgatttcca
    15721 aaaaccttta atttggatat aaaacaatgt gtaaaataag ctcagggcaa atttgggtac
    15781 tagacagcgc gagcttacga atgcgaaata aaatattgaa aaaaatttgt gaagaattat
    15841 ctggataata aaataaaata gtgttattgt aagaaatagc taaataaaaa tatccttcag
    15901 ggtagggtga aattcccaat cggcggtgat gaagttaatt caaagtccgt gagcgtaagc
    15961 tgatttggtg caaatccaaa accgacagtt atagtctgga tgggagaagg ataaaagtat
    16021 gtgcttttta aattcgcttg agtttataag gtttatttga gtacgctttg ttaactactt
    16081 cccatcgtct agttgggaag tagtttttgt tttatttggc taaaaaattg aatagagtag
    16141 ttcttcgggg tagggtgaaa ttcccaatcg gcggtgatga agcaattcaa agtccgtgag
    16201 cgcaagctga tttggtgcaa atccaaaacc gacagtgaca gtctggatgg gagaagaacg
    16261 acgagatgat agcctatgga aaaattttat atccatgagg tttatttgtt gacgtcttat
    16321 gtatatgcac tcccaaaccc cttaaatcta aggggttttt tgtttgaaca aaaatgatta
    16381 aatgaatgaa agtaggtgaa aaaatgttta caggaattgt tgaggatatt ggcaccgtaa
    16441 aaaccttgca aagcgatcga caaagtatga aaattactgt tatttcaaag aaaatggtag
    16501 aggatgtaaa gcttggggac agtatagcgg tcaatggtgt ttgtttaaca gtgatccatt
    16561 ttactgaaca agagctaacg atggatgtca tgcctgagac agtgagcgca acaaatttac
    16621 agcagctcgc aattggagat ccggtaaatt tggagcgtgc aatgtcagct aatggtcgtt
    16681 tcggtggtca tttcgtatcc gggcatgtag acggggtagg gaaagtagtg cgaaagcggc
    16741 cgttagcaaa tgcagtctat attgatatcg agctatcaaa ggagttaacg agcttctgta
    16801 ttccaaaggg ctccatcaca attgatggaa cgagcttaac actcttccac gttgagaata
    16861 atagtgtcac agtatcttta ataccccaca catacaaaga aaccatttta ggtaagaaga
    16921 agattggtga gctcgtcaat gtagaaaccg atttagttgg aaaatacatt ttgcatcaac
    16981 ttaagagtgg acaggaacca ccgaccatta caagggattt cttagcgcaa aacgggtttt
    17041 aacttcgctc agcagaaaga agttaaagcc ttcagcggat gtcacggaat cggaaagaaa
    17101 tatgccgagg cattaattga ttcaacattt tctaacagga ggtggcaata atgctacatt
    17161 caattgaaga agcaatcgag gatttaaaac aaggtaagat cattattgtc gtagatgatg
    17221 aggaccgaga aaacgaaggg gatttactag cacttgcgga atttatgact cccgaaacca
    17281 ttaactttat ggcgacttat ggaagaggtc ttatttgtac accgatttct cagcaacttg
    17341 cacaaaaatt agcattaaat cctatggtaa gtatgaatac agaccatcat caaacggcat
    17401 ttaccgttag tatagatcat gtaaacacat caactggaat cagcgccttt gaacgggcaa
    17461 tgactataca aaaaatgata gaaccccaag caaaagctgc agattttcat agaccaggcc
    17521 atgtatttcc gttaatagca aaggacaatg gtgtattaga gcgtagagga catacagaag
    17581 ctacaattga tttggccaag ctctgtaata gtattcctgc gggcattata tgcgaaataa
    17641 tgggcgacaa tggcaaaatg ctacgtttta acgaccttgt taaatatgca gcacaacatc
    17701 aattaaaaat gatttcaatt gaagcattag taacatatcg tcgtcaacaa ataatggatg
    17761 aaaacacaat aacaatataa aaaaaggctg atcaccaaaa ggtattaata cctataccat
    17821 gtatatagtt gatttccgtt ccggctgggc gactcgtagg gatcagtgtc acggtggagc
    17881 gagcatcata agtaaagcgc tcatcggaca cctccagcgc ccaatcggaa atcaatcagc
    17941 atatggtgat gagccataaa aaggagataa gaaaaatggg aaacactttt gaagcacaat
    18001 taattggtac agatttaaaa atagcggtcg tagtaggtcg tttcaatgaa tttattacaa
    18061 gcaaattatt agacggtgca ttagatggtt taaaacgtca tggtgtggat gagaatgcta
    18121 tcgatgtggc atgggttcca ggtgcatttg aagttccatt catcgcgaaa cagttagctg
    18181 aaacaaaaaa atatgatgca attattggat taggtacagt aatccgtggt tcaacaacac
    18241 attatgatta cgtatgtaat gaatccgcaa aaggtattgc aaatgtgtca ctaactacaa
    18301 atgtacctgt tatttttggc attgttacta cagaaaatat tgaacaagcg attgagcgcg
    18361 cggggactaa atcaggaaat aaaggctatg acgcggcaat gtctgctatt gaaatggcaa
    18421 atttaaaaaa gatgattgga taaatcgtag atcatagaaa ataattctga ggaagtattt
    18481 tgaatatttt tttaaaccat ggagaaaata tccttaagtg tatttatgtt attactcata
    18541 aatgtactaa tttggagctg gctcagtagg agatgaggga tatttcttca atggaacagg
    18601 agttaaattt gaaatctttg aagtcgctat ttcaacgctc tgctgatgtg atatttcaag
    18661 agtatttctt tcaacaacat aaagtacatt tgataaaatg tgaagcgatg atcgatgagc
    18721 ggttactgca gaccgtgata gtacaacggg tacaatcatt atttacggaa gcatcggatg
    18781 aattgttaga ggaacatata caaacaaatt tacacattcc tcatctgcag agaataacaa
    18841 gtaaaaatga tgccatgaca agagtctata aaggacatgt tttactatat tttgaacatc
    18901 tgaatttgtt gtatacaagt tatatcgcta aaaaaccaaa tcgggagcct aaagaaacaa
    18961 ctcttgaatt agttgtcaga ggccctagag atgattttat tgaggatgtt tttatcaata
    19021 ttgctttaat acgaaaacga gtacctacaa attcattaag tgttgaaaca tacgaacttg
    19081 gtaaacgctc caaaacaacc gtagctgtac tttattttga agatgtcgtc aatcaaaaga
    19141 tgcttgagaa aataaaatca cagctgcaac gaattgatgc ggatattgta tttagtgggg
    19201 atttattgat ggaaagaatt gaacagagtg ctaaaatcct tcctagacat gattatactg
    19261 gacgccctga ttatgctgta caagctttaa gtagagggcg agtgattatc atagttgatg
    19321 gtgtatcata cgctgttatt acacctgcca atacaatgtt actctttaaa tccggtgagg
    19381 ataacgaata tccaattatt gtaagttcaa tggagcgtat actccgaata atagcgatct
    19441 taataagtat acttttacca ggtttttggc ttgcactttc aacttaccac caaaatcaat
    19501 tgccattcat tctattggca acagttgttg agtctagaac aggcctccca tttccaacaa
    19561 ttcttgaaac actaatgatg ctttttatgt ttgaaatatt tcgagaggca aatatacatt
    19621 tacccaatgt tattagtggt tccataagtg ttgttggggg gttgataata ggtgacgctg
    19681 ctattaaagc gggtgttacg agcccgtcca tgattgtagt tatagctact tcaagtattg
    19741 ctgcctttac acttggcaat caatctattg tatccgcaat gagcattctc agggtaatta
    19801 ttattttagc atcggccttt tttgggttat ttggattctt cgtttcggtg tttttcatct
    19861 tgctttatac tgccaatata cgggttctag gtgttccata cttaaatatg ggcgcagatt
    19921 taaattggca ggatattaaa aaatctttag ttcgcttatc tccttcaggg tatcgtatgc
    19981 gaccaaaatt tttaaatgta caggatgaaa caaagacatc gagtaagaaa aaaataaata
    20041 cttagcatga agagatcagg tctttatagg cctggtcttt atatgttcta ttttcacttg
    20101 gaagctttat gatgaatata gaaaactgac cacattagat caaaagagaa acgaatattg
    20161 ttcttctttt tagtttacct tctagagaag gatcattaaa ctaatggttg cgacctgtac
    20221 atacgtccct cgaagtaaaa aaattgtttt aaaatttaat cagtccctaa cagcctattc
    20281 aacctgggaa ataagaagga aatagtcaaa tagtaagtgc gaaaaatgtc ggtttttgat
    20341 agccaaaacg ctcatgtagt gataaaatga gaaccaatat tcttatttag agaggcgaag
    20401 aattctgaat tcgtaggtat tttactaata aatcggggag gtgcttttaa gtagggcgag
    20461 tagatagcca atgctgttga ataatcttgc gactttaaaa ggcagagtaa attactacgc
    20521 taatgatgaa atttaaataa tagaattgtt ggatataaat gtttccctag tagaaagtat
    20581 actttgaata agacaactgt tatttttttt atgcgaaaat taag
//
