LOCUS       NZ_CP086322            10846 bp    DNA     linear   CON 26-NOV-2024
DEFINITION  Streptomyces halobius strain 3_2 chromosome, complete genome.
ACCESSION   NZ_CP086322
VERSION     NZ_CP086322.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces halobius
  ORGANISM  Streptomyces halobius
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9239126)
  AUTHORS   Wen,Y., Zhang,W. and Liu,X.G.
  TITLE     Streptomyces nigrumlapis sp.nov.,an antimicrobial producing
            actinobacterium isolated from Black Gobi rocks
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9239126)
  AUTHORS   Zhang,W., Liu,X.G. and Wen,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2021) Northwest Institute of Eco-Environment and
            Resources, CAS, Chinese Academy of Sciences, Donggang West Road 320,
            Lanzhou, Gansu 730000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. FEB-2021
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 241.26X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023277745.1-RS_2024_11_26
            Annotation Date                   :: 11/26/2024 03:43:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,438
            CDSs (total)                      :: 8,346
            Genes (coding)                    :: 7,837
            CDSs (with protein)               :: 7,837
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 509
            CDSs (without protein)            :: 509
            Pseudo Genes (ambiguous residues) :: 0 of 509
            Pseudo Genes (frameshifted)       :: 193 of 509
            Pseudo Genes (incomplete)         :: 418 of 509
            Pseudo Genes (internal stop)      :: 17 of 509
            Pseudo Genes (multiple problems)  :: 113 of 509
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:33:41
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 9001069
            Orig. end                         :: 9011915
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086322.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10846
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="41"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10846
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10846
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5846](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5846)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            240..476
                     /locus_tag="K9S39_RS40830"
                     /old_locus_tag="K9S39_41010"
     CDS             240..476
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF040017.4"
                     /locus_tag="K9S39_RS40830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_41010"
                     /product="DUF6186 family protein"
                     /protein_id="WP_248868323.1"
                     /transl_table=11
                     /translation="MAERPVFGYIVWAVLFGAVFAWEGIALIRAGDVFPTLSDAMRAVM
                     RYPVGRWALFALWLWFGWHTFVRGWHFLLRDGQ"
     gene            507..938
                     /locus_tag="K9S39_RS40835"
                     /old_locus_tag="K9S39_41015"
     CDS             507..938
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF040039.4"
                     /locus_tag="K9S39_RS40835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_41015"
                     /product="DUF6256 family protein"
                     /protein_id="WP_248868324.1"
                     /transl_table=11
                     /translation="MLPTSLNLALMLTGYLLVMGYLGVGLLLLRRPPEHTEQPPRRPGP
                     RRAGAPVPVPARRGWPGLIRQVVGTAIGGYVLLMVAAFGYYYGVVGLGGRFLLSAVTGS
                     ALMVGVSLPLFFIASWVAAWWRKRSSRAADRSGPADSER"
     gene            complement(1004..1225)
                     /locus_tag="K9S39_RS40840"
                     /old_locus_tag="K9S39_41020"
     CDS             complement(1004..1225)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K9S39_RS40840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="K9S39_41020"
                     /product="hypothetical protein"
                     /protein_id="WP_248868325.1"
                     /transl_table=11
                     /translation="MGAAWEWIRVFNRDHPKAGRGDYAGDEAVWPDAISGVCAPSPDTA
                     EHLAVASLADTFDVLVHIRRVSPVRWLP"
     gene            complement(1335..2048)
                     /locus_tag="K9S39_RS40845"
                     /old_locus_tag="K9S39_41025"
     CDS             complement(1335..2048)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004000957.1"
                     /locus_tag="K9S39_RS40845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_41025"
                     /product="hypothetical protein"
                     /protein_id="WP_248868326.1"
                     /transl_table=11
                     /translation="MRKGLEQGTRGDRLTVLREPVWRTVCVYLTLALAGAGIGWLVGLL
                     ADWLVTLPWAPLQGPAELLASIPAPWLPVAGSVAGLVLGLVAQHEQLVIRLSDDRVELI
                     CKGREQEFSHDAVATAFRDGKRLVLIGHDGGELTRQECDLDAGRVADAFAEHGHPWADA
                     DPYEDEFRRWVPESPGLPEGANALLKARQKALEKEGPSDDDVRDLREELARLGVVVKDE
                     KRRQFWRTFRQSGPA"
     gene            2180..2935
                     /locus_tag="K9S39_RS40850"
                     /old_locus_tag="K9S39_41030"
     CDS             2180..2935
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540791.1"
                     /locus_tag="K9S39_RS40850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_41030"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_248869203.1"
                     /transl_table=11
                     /translation="MRTVDPARHRARRRHIVNAAAELFAAKGFERTTTAEICKAAGMSA
                     GNLFHYFPNKRAIFHAVFEDDEHGEADRHDQHGEQVRYDGHDRHGECENAPTKYERLAA
                     ARAADDPLTALLDTVDLLTAPAREPLAPALVMEAMIQAYRDPELEALLSRDNDEERSTL
                     TTVLRNAAAAGQIDPDLDPDDTAAWVMALIAALYINAATDPSFSPADQLPTLRLIIQRF
                     LRPDAVPERATTAGTVTAGATTGGPFPGR"
     gene            complement(3012..4256)
                     /locus_tag="K9S39_RS40855"
                     /old_locus_tag="K9S39_41035"
     CDS             complement(3012..4256)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024306.5"
                     /locus_tag="K9S39_RS40855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_41035"
                     /product="ferritin-like protein"
                     /protein_id="WP_248868327.1"
                     /transl_table=11
                     /translation="MVTATLTKEFVPAVLDIWPVDPKSDKEDMEQLENHLLQAAYLEMS
                     TVPMYLYALLSIETRGYSQWDPGMGASRLIRSIVVEEMLHLSLVRNILVAIGARKKIKF
                     YDKKFVPAYPSLMLHRWPELTLHLGPCSKKLVKDVFMEFELPRQDVTTLEAIPDGWYRT
                     IGEFYQSVGERIKALDKKTKGELWQTKESEFQYVEVYWNKDGGGEPVLVHDLKSAQDAV
                     KIIVEQGEGIDPKSPSVPIDPLNPKPGLDELPHYTKFKRIADGVEPLGNVYNLPTDPKS
                     TDYDKKREPAAHAINTLFNAAYCYTLHMLDVIYETSWKDLAPGKENHRYHLERTFISLM
                     QGVLVTVAETMVASELNIGPLIPQNPGPTFEFYQLPATGKKAHLIDLCEQAMHHFPQLG
                     GDNSVLWLLNKMPDV"
     gene            complement(4328..5008)
                     /locus_tag="K9S39_RS40860"
                     /old_locus_tag="K9S39_41040"
     CDS             complement(4328..5008)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021364.5"
                     /locus_tag="K9S39_RS40860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_41040"
                     /product="DNA-binding domain-containing protein"
                     /protein_id="WP_248868328.1"
                     /transl_table=11
                     /translation="MASPPVTLADTQRWLQHAILTPDGPPDAVDEVLTPSTELSARQRL
                     DVYRRGYRLRLLEAMRELHPALRALLGPELFDDFALDYLDARPSRSYTLCQLDARFVEH
                     LCAHRPDRGLPDGQREGWVDVIVDLARYERAFAEVYDGPGTEGGAPGWSGTVPAPASCL
                     RVLRVCAPVHTYHAAVRRGRPAEPPAPGPVHLALWRRDYTIKVTELEPDAYRLLSARLA
                     EGGA"
     gene            complement(5001..5846)
                     /locus_tag="K9S39_RS40865"
                     /old_locus_tag="K9S39_41045"
     CDS             complement(5001..5846)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /locus_tag="K9S39_RS40865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_41045"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_248868329.1"
                     /sec_met_domain="DUF692 (E-value: 1e-96, bitscore: 322.2,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPESPLGFGVGLRTPHLGHVLGHWPHVDWFEIISENFMDSGGYPR
                     YALDRIAERYPVVMHGVSLSIGSTDPLDLAYLGRLRLLADATGARWVSDHICWTGVAGI
                     NTHDLLPVPFTEESLAHMVRRVRAVQDFLERPLVLENPSSYAEFTGSAMSEWEFVSRLA
                     DESGCGLLLDVNNIYVSSVNHGFDPEEYVRALPHERVVQIHLAGHTDLGTHIIDTHDRP
                     VADPVWRLYRLATSLTGPVPTLLEWDDRIPALPELLDELAKARRYTAAAGNTRARVLCG
                     "
     gene            complement(5924..6499)
                     /locus_tag="K9S39_RS40870"
                     /old_locus_tag="K9S39_41050"
     CDS             complement(5924..6499)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K9S39_RS40870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="K9S39_41050"
                     /product="hypothetical protein"
                     /protein_id="WP_248868330.1"
                     /transl_table=11
                     /translation="MGTPHHNGEKRLSAEEISLLMEGGELGARKRAGARSAAPAFIEPE
                     KLQKEIELHVCMGLNACRRHGVDGSGDMAGMGNCATVFHVCHGANACRAQGGCGYSGSD
                     AEQAKPGNQDCRWNGSCASPINEGRVHAAGPYRGTSVWKRARKIFEERMYEAGIPFGPS
                     PGEGFPDNLVPRYEEKRKIPKGTKGSGV"
     gene            6794..8248
                     /locus_tag="K9S39_RS40875"
                     /old_locus_tag="K9S39_41055"
     CDS             6794..8248
                     /EC_number="1.2.1.12"
                     /GO_function="GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_function="GO:0051287 - NAD binding [Evidence IEA]"
                     /GO_process="GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009333304.1"
                     /locus_tag="K9S39_RS40875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_41055"
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="WP_248868331.1"
                     /transl_table=11
                     /translation="MTVNDDSFTNWKNREEIAESMIPIIGKLHRERDVTVLLHSRSLVN
                     KSVVSILKTHRFARQIAGEELSVTETLPFLQALTALDLGPSQIDIGMLAATYRADDRGL
                     PVAEFTAEAVAGATGADKIERREPRDVVLYGFGRIGRLVARLLIEKSGSGNGLRLRAIV
                     VRGGGGRAAEDLVKRASLLRRDSIHGQFQGTITVDEANSKIIANGNEIKVIYANDPSEV
                     DYTAYGIKDAILVDNTGKWRDREGLSTHLRPGIEKVVLTAPGKGDVPNIVHGVNHDMLK
                     PDEQILSCASCTTNAIVPPLKAMADEYGVRRGHVETVHSFTNDQNLLDNYHKSDRRGRS
                     APLNMVITETGAASAVAKALPDLKAKITGSSIRVPVPDVSIAILNLQLERVTTREEVLG
                     YLRNVSLTSPLKRQIDFISAPDAVSNDFIGSRHASIVDAGATKVEGDNAILYLWYDNEF
                     GYSCQVIRVVQHVSGVEYPTYPAPVV"
     gene            complement(8440..8562)
                     /locus_tag="K9S39_RS40880"
     CDS             complement(8440..8562)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K9S39_RS40880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_248868332.1"
                     /transl_table=11
                     /translation="MSALGRREEREDSPEGYPQTPPYEWWNRHDAYGEDEPAQE"
     gene            complement(8659..9939)
                     /locus_tag="K9S39_RS40885"
                     /old_locus_tag="K9S39_41065"
     CDS             complement(8659..9939)
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016578082.1"
                     /locus_tag="K9S39_RS40885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_41065"
                     /product="methyltransferase"
                     /protein_id="WP_248868333.1"
                     /transl_table=11
                     /translation="MNDPALVRAACLFGPAIATAVAAGLRPPARRTLAAAIVATAWNAV
                     WLPALNLLAVRAGWWTFHAEGGTAAGIPVDLLIGWSVLWGALPVLADGGRIRLPLLVAG
                     LAWLDLAVMPFAAPVVRLGADWLVGEAAAVAAALVPGLLLARWTRRATHLALRAGAQML
                     LSAVLMFALPIALVRPHIPGGTAGLAITVQLLAVPLLLGVIAVREFAERGGGTPLPYDP
                     PQRLVTSGPYAYVRNPMQLSVTLSYALLSLIALDWRLLAAAATSFAYGAGLADWHEAAG
                     LRDAHGERWAAYRAGVRAWLPRLRPWSGTATARLYVAESCGPCSRFGRWVARRHPVALE
                     LVPAEHHPRPLRRMTYETDDGAVRAEGVAALARMLGHLHLGWAVLGWLLVVPGVRWFAQ
                     LCVDALGAGPRDIPHRPQAQPPAATTH"
     misc_feature    10090..10100
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.79"
                     /tool="antismash"
ORIGIN
        1 ccggccggaa cccggagctc gccgtgcgac accgacgcgg ccagtacctc ataacggcgc
       61 cgtctgccgt cggtcataag cggacccgct ccagcccgtg ccgcctcggt cgtcctggtc
      121 ctacgacaga cccggccgca tcagcgtcgt atccaccgct ttgagcccgc tgtgacgcat
      181 gatcgactcg gctgccctgg tacggcgggg cgagtacgcg agtgtggtgg gaggctgtgg
      241 tggcagagcg tccggtgttc ggatacatcg tctgggcggt gctcttcggt gccgtgttcg
      301 cctgggaggg cattgccctg atccgggcgg gcgacgtctt tcccacgctc agcgatgcca
      361 tgcgcgcggt catgcgctat ccggtggggc gatgggcgct gttcgcgctg tggctgtggt
      421 tcggctggca caccttcgtc cgtggctggc acttcctgct gagggacggg caatgacggc
      481 ccacgggtga tcgaagggtc cctggagtgc tccccacctc actgaacctc gccctcatgc
      541 tgaccggtta tctgctggtc atgggctacc tcggggtcgg cctgctcctc ctgcgccgac
      601 ccccggagca caccgaacag cctccccggc ggccggggcc ccggcgcgcc ggggcccctg
      661 tccccgttcc cgctcgacgg ggatggcccg gcctgatccg ccaggtggtc ggcaccgcca
      721 tcggcggata tgtgctgctg atggtcgcgg cgttcggcta ctactacggc gtggtgggct
      781 tgggcggccg atttctgttg agcgcggtca ccggttctgc gctgatggtg ggggtttccc
      841 tgccgctgtt cttcatcgcg tcgtgggtgg ctgcatggtg gcggaagcgg tcgtcccggg
      901 ctgcggaccg gagtgggccg gctgactcgg agcggtagcc ctggtggctg cgggccgggg
      961 ccgatcggta atcctgggtg tcaggctggt cgtcggccac cgctcacggc aaccaccgca
     1021 ccggagacac ccggcggatg tggacgagca cgtcaaaggt gtcggcaagg gaagcgacgg
     1081 cgaggtgctc ggcggtatca ggggacgggg cgcagacgcc gctgatggca tcgggccaca
     1141 cggcctcgtc gcccgcgtag tcgccccgcc cggccttcgg atgatcccgg ttgaacaccc
     1201 ggatccactc ccatgccgcg cccattccgg aggtgcgcca gggtcgtcag gcgtcgtcga
     1261 gctcggattc ggccgcgccc tcgccgccgc gcacgtagcg gtcgaggatg gcggtgacac
     1321 cggtgaggtt gaggtcaagc gggacccgac tgccggaacg tcctccagaa ctgacgtcgc
     1381 ttctcgtcct tcacgacgac accgagacgc gccagctcct cacgaaggtc ccgaacgtca
     1441 tcgtcggagg gcccttcctt ttccaacgcc ttctggcggg ccttgaggag ggcgttcgcc
     1501 ccctccggca gcccgggaga ctccgggacc cagcggcgga actcgtcctc gtacggatcg
     1561 gcatccgccc aggggtggcc atgctcggca aaggcgtcgg caacgcggcc ggcgtccaga
     1621 tcgcactcct gccgagtgag ttcaccacca tcgtggccga tcaggacgag gcgcttgccg
     1681 tcccggaacg ccgtggcgac ggcgtcatgc gagaactcct gctcccggcc cttgcagatg
     1741 agctcgacgc ggtcatcgga caggcggatc accaactgct cgtgctgggc cacgagtccg
     1801 aggaccagcc ctgccaccga tcccgcaacg ggcagccacg gagcgggaat tgacgcaagg
     1861 agctcggccg gcccctgcag cggtgcccag gggagcgtca cgagccagtc ggccaggagg
     1921 ccgacgagcc agccgatacc cgcccccgcc agcgcgagag tgagatagac gcacacggtg
     1981 cgccagaccg gctcgcggag cacggtgagc cggtcacccc gcgtgccctg ctcaagtccc
     2041 ttgcgcatcc tcacgttccc ccttgccccg gccgtacccc tcgtcatgct cgtcattata
     2101 gaccgagcgc tcggtctata atgagccatc agtcatgcag gatccggagg agtcgagagc
     2161 cgacgaggag gaggccacgt tgaggacagt cgacccggcc agacaccggg cacggcgccg
     2221 gcacatcgtc aatgccgcgg ccgaactctt cgccgccaag ggcttcgagc gcaccaccac
     2281 ggcagaaatc tgcaaggccg ccggcatgag cgcgggcaac ctgttccact acttccccaa
     2341 caaacgcgcg atcttccacg ccgttttcga ggatgacgag cacggcgagg ccgaccggca
     2401 cgaccagcat ggagagcagg tccggtacga cgggcacgac cggcacggtg agtgcgaaaa
     2461 cgcccccacc aagtacgagc gtctcgctgc cgctcgtgcc gccgacgatc cgttgaccgc
     2521 cctcctcgac accgtcgacc ttctcaccgc ccccgccagg gagccgctgg ccccggccct
     2581 cgtcatggag gcgatgatcc aggcataccg cgaccccgaa ctcgaagcgc tgctgagccg
     2641 ggacaacgac gaggagcggt ccaccctcac caccgtgctg cggaacgcgg ccgcagcggg
     2701 ccagatcgac cccgacctcg acccggacga caccgcagcc tgggtcatgg cgctcatcgc
     2761 cgctctctac atcaacgccg ccaccgatcc gtccttcagt ccggccgacc aactccccac
     2821 cctgcgcctg atcatccagc gcttcctacg gcccgacgcc gtcccggagc gagcgacaac
     2881 cgccggaacg gtaactgctg gagcgacaac cggcgggccc ttcccgggaa ggtaggacga
     2941 ggtgacctgc gcgcgacagc ggtggtggcc cgccccggcc ctggggcagg ccaccaccgc
     3001 gtgccggcgg atcagacgtc cggcatcttg ttgagcagcc agagcacgct gttgtccccg
     3061 ccgagctggg ggaagtggtg catggcctgc tcgcagagat cgatgaggtg ggccttcttg
     3121 cccgttgcgg gcagctggta gaactcgaac gtcggccccg ggttctgggg aatgaggggg
     3181 ccgatgttga gctcgctggc gaccatggtc tcggccacgg tcaccagcac gccctgcatc
     3241 agggaaatga acgtgcgctc caggtgatag cggtgattct ccttgcccgg cgcgagatct
     3301 ttccacgacg tctcgtagat gacgtccagc atgtgcaacg tgtagcaata cgccgcgttg
     3361 aacagcgtgt tgatggcgtg tgccgcgggc tctctcttct tgtcgtagtc cgtggacttc
     3421 gggtccgtgg gaaggttgta gacgttcccc agcggctcga ccccgtcggc gatccgcttg
     3481 aacttcgtgt agtggggcag ttcatcgagt ccgggtttcg ggttgagcgg gtcgatcggc
     3541 accgaggggc tcttgggatc gatcccttcc ccctgctcca cgatgatttt cacggcgtcc
     3601 tgcgcgctct tcagatcgtg cacgaggacc ggctcgccac cgccgtcctt gttccagtac
     3661 acctccacgt actggaattc cgattccttg gtctgccaga gctcgccctt ggtcttcttg
     3721 tcgagggcct tgatacgttc ccccacggac tggtagaatt cgccgatggt ccggtaccag
     3781 ccgtccggga tggcctccag agtggtcacg tcctgtctcg ggagttcgaa ctccatgaag
     3841 acgtccttga ccaatttctt gctgcagggc cccagatgca aggtcagctc gggccagcgg
     3901 tgcagcatga gcgacggata ggctggcacg aatttcttgt cgtagaactt gatcttcttc
     3961 cgggcgccga tcgcgaccag aatgttccgc accagggaca gatgcagcat ctcctcgacc
     4021 acgatgctgc ggatcagccg cgacgcaccc attcccggat cccactggga gtagccccgc
     4081 gtctcgatcg agagcagggc gtacagatac atcgggaccg tcgacatctc cagataggcg
     4141 gcctggagca ggtgattctc cagctgctcc atatcctcct tgtcgctttt ggggtccacc
     4201 ggccagatgt ccaggacggc tggcacgaat tccttggtga gagttgcggt gaccatcgtg
     4261 gatgctcctt cactcacgtt cattcactca tggggaactg acgtcatggc atggccgggg
     4321 agcgtcgtca tgcgccgcct tcggcgagcc gtgcggacag caaccgatag gcgtccggct
     4381 ccagttcggt caccttgatc gtgtagtcgc gccgccaaag agccaggtgt acgggccccg
     4441 gcgcgggcgg ttcggcgggc cgcccgcgcc ggaccgccgc gtggtaggtg tgcacgggcg
     4501 cacagacacg cagcacccgc agacacgaag ccggtgccgg cacggtgccg gaccatcctg
     4561 gcgccccgcc ctcggtgccg gggccgtcgt agacctcggc gaaggcccgc tcatagcggg
     4621 cgagatcgac gatcacgtcc acccagccct cgcgttggcc gtccggcaga ccgcggtcgg
     4681 gccgatgggc gcagaggtgc tcgacgaacc gtgcgtccag ctggcagagg gtgtacgagc
     4741 gggaggggcg ggcgtcgagg tagtcgagcg cgaaatcgtc gaacagctcg gggcccagta
     4801 gggcccgcag ggcggggtgc agctcgcgca tcgcctccag cagacgcagg cggtagccgc
     4861 gccggtagac gtcgagccgc tgccgggcgc tcaactcggt ggaaggggtg agcacttcgt
     4921 cgacggcgtc cggcggcccg tccggagtga ggatggcgtg ctgcagccag cgctgggtgt
     4981 cggccagggt gaccggtgga ctagccacag agcacccgcg ccctcgtgtt cccggccgcg
     5041 gcggtgtacc ggcgtgcctt ggccagctcg tcgagcagtt cggggagggc ggggatgcgg
     5101 tcgtcccatt cgagcagggt ggggaccgga cccgtcagag aggtcgccag ccggtacagc
     5161 cgccacaccg ggtcggccac cggacggtcg tgggtgtcga tgatgtgggt gccgaggtcg
     5221 gtgtggccgg cgaggtggat ctgcaccacg cgctcgtgcg gcagcgcccg tacgtactcc
     5281 tccgggtcga agccgtggtt gacggaggag acatagatgt tgttgacgtc cagcagcagt
     5341 ccgcacccgg actcgtcggc cagccgggag acgaactccc actcgctcat cgccgacccg
     5401 gtgaactccg cgtagctgct ggggttctcc agtaccagcg gacgttccag gaagtcctgt
     5461 acggcccgca cccggcggac catatgggcc aatgactcct cggtgaacgg gaccggcagc
     5521 aggtcgtggg tgttgatccc ggccacgccc gtccagcaga tgtggtcgga gacccagcgc
     5581 gccccggtcg cgtctgccag gaggcgcagc cggcccagat aggcgaggtc gagcggatcg
     5641 gtgctgccga tcgacagcga gaccccgtgc atgaccaccg gatagcgctc cgcgatccgg
     5701 tcgagcgcgt agcgcggata gccgcccgag tccatgaagt tctcggagat gatctcgaac
     5761 cagtccacgt gcggccagtg ccccagcaca tgtccgagat gcggggtgcg cagcccgacg
     5821 ccgaatccca aggggctttc cggcaatccc aagcctcttt ccggcaaggg ggacggggta
     5881 ttccgtcgcg tcgcgggccc ggaggccggg acatcgccga cggtcatacg ccggagccct
     5941 tggtgccttt gggaatcttc cgcttctctt cgtacctggg caccaggttg tccgggaaac
     6001 cctcgccggg agaggggccg aagggaatcc cggcctcata catccgttcc tcgaagatct
     6061 tgcgggcccg tttccatacg ctcgtgccgc gatagggccc ggcggcatgc accctcccct
     6121 cgttgatggg gctggcgcag ctgccgttcc aacggcaatc ctgattaccg ggtttggcct
     6181 gctccgcgtc gctgccgctg taaccgcaac cgccctgcgc ccggcacgcg ttggcgccgt
     6241 gacagacgtg gaagacggtc gcgcagttcc ccattccggc catgtcgccg gacccgtcga
     6301 cgccgtgcct ccggcaggcg ttcagcccca tgcacacatg gagctcgatc tccttctgca
     6361 atttctccgg ctcgatgaac gccggagcgg ccgacctggc ccccgcccgc tttctggcgc
     6421 cgagctctcc gccctccatg agtagggaga tttcctcggc gctcaagcgc ttctcgccgt
     6481 tatggtgcgg tgtgcccatt tccgctctcc tcattctctg tctccggccc catgtgggga
     6541 cgggccggag ctggataccc ggagttgcct tccagcgagt accggccagg aaacgagtca
     6601 taagggtgaa atgagatctt tcaggcgctt ggtcgagcct ggagcctggt cgacgacctg
     6661 acgcatgagc ggggggcaga gcggtcaaaa cctgcaatct cggaccccgc atcccgctca
     6721 cgtggtaccg agtgaaaggg cgaaaagccg gggggttagc atatgagcac cgcctagctc
     6781 gaaagataag cctgtgactg tcaatgacga ctcgttcacc aattggaaga accgcgagga
     6841 gatcgcggag tcgatgatcc cgatcatcgg gaagctgcac cgggagcggg acgtcacggt
     6901 cctgctgcac agccgctcct tggtgaacaa gtcggtggtc agcatcctca agacccaccg
     6961 attcgcccgg cagatagcgg gggaggagct ctcggtcacc gagacgctgc cgttcctgca
     7021 ggccctcacc gcgctcgacc tcggcccgtc ccagatcgac atcggcatgc tcgccgcgac
     7081 ctacagggcc gacgaccgcg gtctcccggt ggcggagttc accgccgagg ccgtcgccgg
     7141 ggccacgggg gccgacaaga tcgagcgtcg cgaaccgcgc gacgtcgtcc tctacggctt
     7201 cggccgtatc ggccgcctcg tcgcccgtct gctgatcgag aagtccggct ccggcaacgg
     7261 cctgcggctg cgtgccatcg tcgtccgcgg aggcggcggc cgggccgccg aggatctcgt
     7321 caagcgcgcc tcgctgctgc gccgtgactc catccacggc cagttccagg gcacgatcac
     7381 cgtcgacgag gcgaacagca agatcatcgc caacggcaac gagatcaagg tgatctacgc
     7441 caacgacccc tcggaggtcg actacacggc gtacggcatc aaggacgcca tcctcgtcga
     7501 caacaccggc aagtggcgcg accgcgaggg cctgtcgacg cacctccgcc cgggcatcga
     7561 gaaggtcgtc ctgaccgcgc cgggcaaggg cgacgtcccg aacatcgtcc acggtgtcaa
     7621 ccacgacatg ctcaagccgg acgagcagat cctttcctgt gcgtcctgca ccaccaacgc
     7681 gatcgtcccg ccgctgaagg cgatggcgga cgagtacggc gtgcggcgcg gccacgtgga
     7741 gaccgtccac tcgttcacca acgaccagaa cctgctggac aactaccaca agtccgaccg
     7801 ccgcggccgc tcggcaccgc tcaacatggt catcaccgag accggtgccg cctccgccgt
     7861 cgccaaggcg ctgcccgacc tcaaggcgaa gatcaccggc agctcgatcc gggtcccggt
     7921 gccggatgtc tcgatcgcga tcctcaacct gcagctcgaa cgcgtcacga cgcgcgagga
     7981 ggtcctcggc tacctccgca atgtgtcgct gacctcgccg ctcaagcgtc agatcgactt
     8041 catcagcgcc cccgacgcgg tttcgaacga cttcatcggc tcgcgccacg cctcgatcgt
     8101 cgacgccggc gcgaccaagg tcgagggcga caacgcgatc ctctacctct ggtacgacaa
     8161 cgagttcggc tactcctgcc aggtcatccg cgtcgtccag cacgtctccg gggtggagta
     8221 cccgacctac ccggcaccgg tggtctgatt ccggtggcct gatcccggtg gtcaggaccg
     8281 gtccggtacg gagatccggc gggttgggcg gcagtggggc ggtaaccgat tccgatcggt
     8341 taccgcccca ctgccagttg ccgctctccg gccgccgccg gcaccgcccc ggcagactca
     8401 gtccccttgg ccgcggcggc ctcggcgagt ccggtccggc tactcctgcg ccggctcgtc
     8461 ctcaccgtat gcgtcgtgcc ggttccacca ttcgtacggc ggggtctgcg gatagccctc
     8521 gggcgagtcc tcccgttcct cccgccgccc gagcgcggac atgtcgaggc agctccaggt
     8581 gctctcccca acgcctcgtc gccgcgggcg ttgatgaaat aggacctccg gcatcacggt
     8641 gcccgtccgc atcagccgtc agtgcgtcgt ggcagcgggc ggctgcgcct gcgggcggtg
     8701 cgggatgtcg cgcggtcccg cccccagcgc gtccacgcac agctgggcga accagcgcac
     8761 ccccggcacc accaacagcc accccagcac cgcccacccc agatgcagat gccccagcat
     8821 ccgggcgagc gccgcgacgc cctccgcccg tacggcgccg tcgtccgtct cgtacgtcat
     8881 ccggcgcagc ggtcgtgggt ggtgctcggc cggtacgagt tccagggcga ccgggtgccg
     8941 gcgcgccacc cagcggccga accggctgca cgggccgcag ctttccgcga cgtacagccg
     9001 ggcggtcgcc gtgccgctcc acggccgcag gcgcggcagc cacgcgcgta cgcccgcgcg
     9061 gtaggcggcc caccgctcgc cgtgggcgtc acgcagcccg gccgcctcgt gccagtcggc
     9121 gagcccggcg ccgtacgcga aggacgtggc cgcggcggcg agcagtcgcc agtcaagggc
     9181 gatcaggctg agcagtgcgt agctgagggt gacggacagc tgcatcggat tgcggacgta
     9241 tgcgtacggg ccgctggtga cgagccgttg cggcggatcg taggggaggg gcgtgccgcc
     9301 gccgcgctcg gcgaattccc gtacggcgat gacgcccagg aggaggggca cggcgaggag
     9361 ctgcaccgtg atcgcgaggc cggccgtgcc accgggaatg tggggacgga ccagggcgat
     9421 cgggagcgcg aacatcagca ccgcgctgag cagcatctgg gcgccggcac gcagggcgag
     9481 gtgggtcgcc cgccgggtcc agcgggcgag cagcaggccg ggcaccaggg ctgcggcaac
     9541 cgccgccgcc tcgcccacga gccagtcggc gccgagccgt acgacgggtg cggcgaacgg
     9601 catcacggcg aggtcgagcc aggcgagtcc cgccaccagc agcggaagcc gaatccgccc
     9661 gccgtccgcg aggacgggca gcgcgcccca cagcacggac cagccgatca gcaggtcgac
     9721 ggggatcccc gcggccgtgc cgccctcggc gtggaaggtc caccagccgg cccgcacggc
     9781 aaggaggttg agggcgggca gccagacggc gttccaggcg gtggccacga tcgcggcggc
     9841 gagggtccgg cgcgcgggcg gtcgcagccc ggctgccacg gccgtggcga tggccggccc
     9901 gaagaggcag gcggcgcgga ccagcgcggg gtcgttcatc gtgccgtggc cccgaaggtg
     9961 tcggagagat acgcggccgc ctgctccatg gcgtactgct ggacggggcc gaagtaccag
    10021 ctcgggtcgt aggtgcgccg gtagtcgagg gtccaggtga cgtcggtgcg gcggccgtcc
    10081 gcccggggct tccatgtcac ctcggcggat tcgatgtcca gccagcgggc gtaggtcgtg
    10141 tcgcgctcga cggcgaagac gacgcggccg ggggcgcgtt cggtgatgac gaggcgcatg
    10201 gagcgcgggg tggggcggga gccgaagccg agggacttgc gtggagagaa ggtgatctcc
    10261 cggtggtcgc cgacgctcag gccggtgccc gtggacgact gcgggcgggg gaaggggatc
    10321 tggaggaaca cgggccgggg cgtgtcgaag cggggaggcg cggcgagggc ggcctcgtag
    10381 cgcgccggag tcaggtccac ggtacgggtg acggaggcgg cgttgtcgcg cgggagctcg
    10441 tacgcccccg tgccctccat cgtcagcacg gcgagcaggg gcagggtgac gaaggcgtgc
    10501 gcgcgcccgc cgcgcccctc gcgctccctg cgccgggtgc cgtcgacgat caggccgatg
    10561 gccaccgcga gcagatagaa cagcggggcg gcgaagacca ggcagatcac cccctcgtcg
    10621 agcagcggcc cggcgagggc cagcccgatg gtgacggcgg cgagcgcgct gccggtggcg
    10681 ctcctgggcc gggcgccgat ggccacggcg agggcgatga aggcgggcag gccgacgtag
    10741 aagagagcgg tctgctccag atgcccggcg tgcaggacct tgtagagcag catcgcggcg
    10801 aacaggacgg cgagcacgcc gatcaaccgc caccgtgccc gcgaga
//
