LOCUS       NZ_CP086322            10867 bp    DNA     linear   CON 26-NOV-2024
DEFINITION  Streptomyces halobius strain 3_2 chromosome, complete genome.
ACCESSION   NZ_CP086322
VERSION     NZ_CP086322.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces halobius
  ORGANISM  Streptomyces halobius
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9239126)
  AUTHORS   Wen,Y., Zhang,W. and Liu,X.G.
  TITLE     Streptomyces nigrumlapis sp.nov.,an antimicrobial producing
            actinobacterium isolated from Black Gobi rocks
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9239126)
  AUTHORS   Zhang,W., Liu,X.G. and Wen,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2021) Northwest Institute of Eco-Environment and
            Resources, CAS, Chinese Academy of Sciences, Donggang West Road 320,
            Lanzhou, Gansu 730000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. FEB-2021
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 241.26X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023277745.1-RS_2024_11_26
            Annotation Date                   :: 11/26/2024 03:43:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,438
            CDSs (total)                      :: 8,346
            Genes (coding)                    :: 7,837
            CDSs (with protein)               :: 7,837
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 509
            CDSs (without protein)            :: 509
            Pseudo Genes (ambiguous residues) :: 0 of 509
            Pseudo Genes (frameshifted)       :: 193 of 509
            Pseudo Genes (incomplete)         :: 418 of 509
            Pseudo Genes (internal stop)      :: 17 of 509
            Pseudo Genes (multiple problems)  :: 113 of 509
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:33:41
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8603246
            Orig. end                         :: 8614113
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086322.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10867
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="36"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10867
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10867
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5867](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5867)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            75..242
                     /locus_tag="K9S39_RS43225"
                     /old_locus_tag="K9S39_39195"
     CDS             75..242
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014182431.1"
                     /locus_tag="K9S39_RS43225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_39195"
                     /product="hypothetical protein"
                     /protein_id="WP_248868011.1"
                     /transl_table=11
                     /translation="MRAADGPVQVRGVGEELGLEVAVRGKLEPLRAKMTKLADRGWLHK
                     RPDGRFTARR"
     gene            complement(390..827)
                     /locus_tag="K9S39_RS39030"
                     /old_locus_tag="K9S39_39200"
     CDS             complement(390..827)
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016430.5"
                     /locus_tag="K9S39_RS39030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_39200"
                     /product="RNA polymerase sigma factor"
                     /protein_id="WP_248868012.1"
                     /transl_table=11
                     /translation="MVGDGLSQEAVALGGHVAQRLLDCYPVFMETEPAALRRAFRVLSL
                     AACQDIAQEAYLRVGSKADAGKLPPGTNVMAYLRRTARNLALDRCREQQRGRRLVLMSG
                     DALDAVTEPRAVADDGPRVLQELVIPAIEGMPESLRRKAAR"
     gene            complement(1240..1464)
                     /locus_tag="K9S39_RS39035"
                     /old_locus_tag="K9S39_39205"
     CDS             complement(1240..1464)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018520531.1"
                     /locus_tag="K9S39_RS39035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_39205"
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="WP_248861517.1"
                     /transl_table=11
                     /translation="MPSTFDRPRRITRDYDGPLLVEGPVEFICGDGSVRISRRLAVAVC
                     TCRRSRAYPWCDTSHRRRTEPDAQVEAKG"
     gene            complement(1448..1645)
                     /locus_tag="K9S39_RS39040"
                     /old_locus_tag="K9S39_39210"
     CDS             complement(1448..1645)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K9S39_RS39040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="K9S39_39210"
                     /product="hypothetical protein"
                     /protein_id="WP_406707858.1"
                     /transl_table=11
                     /translation="MVHSALCGVDATLHRLAEVGLRPEVADRTVIPLGPVLRCRQAWLR
                     RQGLLSDSEEGLVIIRAEHF"
     gene            1966..3501
                     /locus_tag="K9S39_RS39045"
                     /old_locus_tag="K9S39_39215"
     CDS             1966..3501
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049447.1"
                     /locus_tag="K9S39_RS39045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_39215"
                     /product="bifunctional phosphatase PAP2/diacylglycerol
                     kinase family protein"
                     /protein_id="WP_248861516.1"
                     /transl_table=11
                     /translation="MDEQPTRHPVRTLLRTLDHAVFRRVGERSWPMAEPVLPRLSRSAN
                     HGLLWGGVAVGMWALGGTRGRRAAVRGVASLALVSATVNTLGKGAVRRKRPVLDAVPVV
                     RHLPRQPVTSSFPSGHAASAVAFTVGVALESRGWGAALAPVAASVAFSRIYTGVHYPSD
                     VLVGSALGVGAAFAVRGLVPSRAQLPPPARPRTDAPALPDGDGLYVVVNPSSGAQPQLM
                     DSVRQLKSELPRAEIIVHEEGGGPLPKVISDAAREAARRGGALGVCGGDGTINAAVAPA
                     LQFQVPLVVLPSGTFNHFAADLGTETVAAACAAVANGSAVRVDVGRITPLAARSAEPTC
                     FLNTFSLGSYPDLIRLREHWSPKIGDPAATLLGVAKILHTAHPLKAVVNGRRRAMWLLF
                     AGNGAYRNLGIAPVRRHDLTDGLLDVRIAHGGRFARTRLLATVLAGGVARTRLYAAAHS
                     RQLLISGLPEGARMAYDGEVVEAPTAFQVDKLQEALTVYRPLEERPRARPMSG"
     gene            complement(3742..3864)
                     /locus_tag="K9S39_RS42215"
     CDS             complement(3742..3864)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K9S39_RS42215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_283113266.1"
                     /transl_table=11
                     /translation="MADDSEELFRDALKAGLAQSGPAVRFGGLFVLLLLPFLNA"
     misc_feature    3919..3932
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.7"
                     /tool="antismash"
     gene            3979..4542
                     /locus_tag="K9S39_RS39050"
                     /old_locus_tag="K9S39_39220"
     CDS             3979..4542
                     /EC_number="3.1.3.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024811.5"
                     /locus_tag="K9S39_RS39050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_39220"
                     /product="HAD family hydrolase"
                     /protein_id="WP_248868013.1"
                     /sec_met_domain="HAD_2 (E-value: 1.9e-13, bitscore: 49.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDAMLSGLEAGLSDPDVVEAQRARDVSSEAHRHAFTTWYASVPEL
                     APVAEGLYGQLQTPEHWVPYVDTASTLTCLADHGVALGVVSDVGWDLRKTFAHHGLDGY
                     FSTWVHSYEHNTEKPNPLLFHHACRELGVEPAETLMVGDHPAKDGGAVGAGLRSYVLPA
                     GAAPGSHRGLDAVLRLAGFDSSGL"
     gene            complement(5001..5867)
                     /locus_tag="K9S39_RS39055"
                     /old_locus_tag="K9S39_39225"
     CDS             complement(5001..5867)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041155.2"
                     /locus_tag="K9S39_RS39055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_39225"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_248868014.1"
                     /sec_met_domain="Linocin_M18 (E-value: 7.3e-58, bitscore:
                     194.6, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTINHLLRGLAPITPRVWQVIEDEARNRLIPLLAARQVVSFAGP
                     GGWEQDAVPTGKATELNTYPDKLTPSGTNVKALRVVQPLTQVRIPFTLPLKAIEEIERG
                     SINPDLTQLDKAAKLAADFENYAVFNGWDRAFIAGIVNQASPSGVTLPVATDTYPADIA
                     KVVDKLRTSGVVGPYTLAIGTTGYQRIIADAGSCCYVLSERLTEILGGKIVLMPCLDEK
                     TGKDTQAVVLSERGGDFLLIVGQDLSIGYLPTTSNTDVSLYLEETFTFRVARPSAALTV
                     TVVAAPV"
     gene            complement(5873..6637)
                     /locus_tag="K9S39_RS39060"
                     /old_locus_tag="K9S39_39230"
     CDS             complement(5873..6637)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K9S39_RS39060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="K9S39_39230"
                     /product="hypothetical protein"
                     /protein_id="WP_248869251.1"
                     /transl_table=11
                     /translation="MELSSDGRARATAAAGTGGYGGAGGYGGAGGYAGAGGYGAVGGYG
                     GAGSAATATGGVGGAEGGGRGGSADAEAVNFGTGGDGGPGSSGGDAGSGGTGGSGGTAG
                     GGGTGGSGGGAGDGSLGIGGIGGTGGYAGAGGYGGTGGYAGAGGYAGAGAAATATGGAG
                     GAEGGNDGGNARARSLNFGTGGRGGAAGPGGDGGSGGAAGPGGDGGSGGRGAAGGSGSA
                     TGSAGGTSAREDGAATSTGGDGSLAIGNLKER"
     gene            complement(6725..7621)
                     /locus_tag="K9S39_RS43230"
     CDS             complement(6725..7621)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1213: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K9S39_RS43230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_406708093.1"
                     /transl_table=11
                     /translation="MRKRRPQIVLEAKEGRRHLEGTVPQEKRPGEEAEVDFADVWLDLA
                     GQRRKCVLFTLRMSYSGKAIHRVYTTASQEAFFEGHVEAFRALGGVPSVHIRYDNLKPA
                     VKHVLFGRSRTETTRWVAFRSWYAFSPLYCGPGEEGAHEKGGVEHEGGRFSPFAEWDKI
                     FADPRLCAAIGDRITFRCTLIQTAPSPTDTRPPRPNARRSQLSDRRQVHPRSGSVPPAP
                     RGEPLALPCQCSLDLRHRQPGCLSDPVDPEAIGVVIEYPGDLAHPPRCPAEPDGHRGEA
                     LTTRPIGPTGHPSRPQR"
     misc_feature    7923..7936
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     misc_feature    8044..8058
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.91"
                     /tool="antismash"
     gene            8592..9443
                     /locus_tag="K9S39_RS39080"
                     /old_locus_tag="K9S39_39250"
     CDS             8592..9443
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015338.5"
                     /locus_tag="K9S39_RS39080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_39250"
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="WP_248868016.1"
                     /transl_table=11
                     /translation="MEIVAMSQNLGPGAWRSQNGIFEDRWPALAATIQRVQPDILMLQE
                     AEGWPADGHARLIRAEADLEMDGVLAPSPSGLGPAIFYRRATMGRRTYQNHDYSAHETH
                     HGFETLGWNLPVLPATLPAGSVHLTPYGGPRAESQVSFIGTRVLRPGVGYAILGGDFNY
                     APVAGPPPLVGAMRPYNKGSRLKVTDPESPGPDEPDRAVAWRIARKGFVDVAWHLYKQT
                     GDEELLRRTATDDRIDWILISFALAPCIVDYGVVEGGSDHAGVWARLDLSLAATDNVWE
                     YK"
     gene            9443..9892
                     /locus_tag="K9S39_RS39085"
                     /old_locus_tag="K9S39_39255"
     CDS             9443..9892
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K9S39_RS39085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="K9S39_39255"
                     /product="hypothetical protein"
                     /protein_id="WP_248868017.1"
                     /transl_table=11
                     /translation="MAEPMAADDILRACGYLEAVWMEDATNMAALLNHRTGEIPTAILV
                     TELGESFMQQALPAQFGVHDDMTPQELQDATEKMTADPTVRVSKVLIEALKEIAPTATP
                     PQAETIARALITYLLAISDATQDDVMPMLTALRQSALQRYGGQGT"
     gene            complement(10386..10823)
                     /locus_tag="K9S39_RS39090"
                     /old_locus_tag="K9S39_39260"
     CDS             complement(10386..10823)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K9S39_RS39090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="K9S39_39260"
                     /product="hypothetical protein"
                     /protein_id="WP_248868018.1"
                     /transl_table=11
                     /translation="MVLTVYADRTGRLWRIAEMCARCAAATPNCRVLDTAPPPTRTAPA
                     GTDQAAGTPDAEPAGGTVGDHVAAVFSDHGPSPAAGASSSPTAAPGSVPPLANCPEASR
                     RAPWWEPRSPLRVMVFGYCLDLRSEATHFMRQPRHRGCLMK"
ORIGIN
        1 tgctgatccc gcaccacggc gagaatcccg acggggtcgc cctgccggac gactaccgca
       61 ggatcctggc gatcgtgcgg gccgccgacg gccccgtcca ggtcaggggc gtcggcgagg
      121 agctgggtct tgaggttgcg gtgcgtggga agctggagcc gctgcgcgcg aagatgacca
      181 agctcgccga tcggggctgg ctgcacaaac gccctgacgg ccggttcacc gcacgccggt
      241 gaccgtgctg gcggaggaat ccggcaggcc gtaactggcg ctccttcgac ggcagttggg
      301 tttggtgtga agaaaaacaa cgggtccgcc gagggccctg acggccttgt ctgcaccgcc
      361 cgcctgccgc tgtcgagcgc caccctgaac tatctcgccg cctttcgtcg caggctttcc
      421 ggcatcccct cgatcgccgg gatcaccagc tcctgcagca cccttggccc gtcatccgct
      481 actgcccggg gctctgtcac agcatcgagt gcgtcgccgc tcatcagcac cagccgtctg
      541 ccgcgctgct gctcgcggca ccggtcgagg gcgagattgc gggctgtccg ccgcagatac
      601 gccatcacgt tcgtccccgg cggcagcttt ccggcgtccg ccttcgaccc cacccgcaga
      661 taggcctcct gcgcgatgtc ctggcacgca gccagcgaca gcacccgaaa cgcccgccgc
      721 agcgccgcag gctcggtctc catgaagacc gggtagcagt ccagcagccg ctgcgccaca
      781 tgccctccca gcgccaccgc ctcttgggac aggccgtctc ctaccacggg cccggcccct
      841 cacccgccgt cgcgggacca agccatgcgc gccctcacct agggcgtcga tccatgcgcc
      901 ctgtgccgcc ccgacaccga gctcgggatc ttctaatcgc gcacggcggg ataacgggga
      961 gcggcgctgg ctgggccgga ggtcgtggtg ccgtgctgtg actgagcgct tcgcttggcg
     1021 gatgagcgtt tgagctggcg gggttgagcg tccgagttgg cgcaggccga gcagggctgc
     1081 agatcgtcgc cgtacggatc cgcagccccg gccccgggcg gcggcgcggg tgccgcaccg
     1141 gtccgcagcg cggccacgat gccggcggag atctcgccgc agggctcagg gcagtggcgg
     1201 gctgctgtgg cgtgcctcga caggggcggg catcagcgcc taccccttgg cttcgacttg
     1261 ggcatcgggc tcggtgcggc ggcggtggct ggtgtcgcac cacggatagg cccggctgcg
     1321 tcgacaggta cagacggcga cggcgagacg ccgcgagatg cggacgctgc cgtcgccgca
     1381 gatgaattcg accggccctt ccacgagcag cggcccgtca tagtcccggg tgatgcgccg
     1441 cggccggtca aaagtgctcg gcacggataa tcaccagccc ttcctcgctg tcgctcaaca
     1501 agccctgccg acgcagccat gcctgccggc agcgcagtac cggaccgagt ggaatgaccg
     1561 tgcggtcggc gacctcgggc cgcaggccca cctcggcgag ccggtgcagg gtggcgtcaa
     1621 cgccgcacag ggcggagtgc accacgagca gcaccctccg ggccccagaa cgttcggcgc
     1681 cgcgtcgcag atccggtcca ccaccccgcg gccgtcgggc ccggcgtccc aggcgcgcgc
     1741 ctgtgctgag gacgggcccg ggcaaagccg atcgacggtg tacatggttc tcgtcgtaga
     1801 agaacccgac ggcagcgggg ccccgcagag ctatggcggg cttgcccagg agccgtgcgc
     1861 ggacggcgcc tggctcctca cggcgggcca agtcggggaa cgcccccaac gtcacctcga
     1921 actgattgcc ctacacaagc aatgatattg ggtataagga ggcacatgga tgaacaaccg
     1981 acgaggcacc ccgtccgcac gctgctcagg accctggacc acgccgtctt ccgccgcgtg
     2041 ggcgagcgtt cctggccgat ggccgagccg gtgcttccgc gtctgagccg gagcgccaac
     2101 cacgggctgc tgtggggtgg tgtcgccgta gggatgtggg ccctgggcgg tacgcggggg
     2161 cggcgggcag ccgtgcgcgg agtcgcgtcg ctcgcgctgg tctcggccac cgtcaacact
     2221 ttgggcaagg gtgcggtgcg ccggaagcgg cccgtgctcg acgcggtgcc ggtcgtacgc
     2281 catctgcccc gccagcctgt caccagctcg ttcccgtctg ggcatgccgc gtccgcggtg
     2341 gcgttcaccg tcggcgtggc cctcgagtcc cgtggctggg gcgcggccct ggcgcccgtg
     2401 gccgcgtcgg tggccttctc ccgtatctat accggcgtgc actaccccag cgatgtcctg
     2461 gtcggttcgg ctctcggtgt gggcgccgcc ttcgccgtgc gcggcttggt gccctcgcgc
     2521 gcgcagctac cgccgccggc ccgacctcgc acggacgcgc ctgcgctgcc cgacggtgac
     2581 ggcctgtacg tggtggtcaa cccgtcctcc ggggcccagc cgcaactcat ggactctgta
     2641 aggcagttga agtccgagtt gccccgcgct gagatcatcg tccacgagga gggaggcgga
     2701 ccgctgccca aggtgatctc cgatgccgca cgcgaagcag cgcgccgggg cggtgccctc
     2761 ggtgtgtgcg gcggcgacgg caccatcaac gcggccgtgg ctcccgccct gcagttccag
     2821 gtgccgctcg tggtcctgcc cagtggcacc ttcaaccact tcgccgccga cctcggcacc
     2881 gagacagtgg ccgccgcctg cgccgcggtc gcgaacggca gcgcggtccg cgtcgacgtc
     2941 ggccggatca cccccttggc cgccagatcg gcagagccga cctgcttcct gaacaccttc
     3001 agcctcggct cgtaccctga tctcatacgc ctcagggaac actggtcgcc gaagatcggc
     3061 gacccggccg ccaccctcct cggcgtcgcg aagatcctgc acaccgcaca tcccttgaag
     3121 gccgtcgtca acggccgccg tcgcgcgatg tggctgcttt tcgcgggcaa cggcgcctac
     3181 cgcaacctgg gcatcgcccc ggtacgtcgc cacgatctca ccgatggcct cctcgacgta
     3241 cgcatcgccc acggcggccg cttcgcgcgc acccgtctgc tcgccacggt actggccggg
     3301 ggagtggcca ggacccgcct gtacgctgcg gcgcactcgc ggcagctgct tatttccgga
     3361 ctgccggaag gtgcacggat ggcatacgac ggggaagtcg tcgaagcgcc caccgcgttc
     3421 caggtcgaca agctccagga ggcactgacc gtgtaccggc cgctggagga acggccgcgt
     3481 gctcgaccga tgagcggttg actgcggacg ggtgaacccg gggaggtgcg ttgcggagac
     3541 gggtccggtc tcgctcgaac tgcaacaccc catcattcgc gcttgctggt gatcgccata
     3601 tggcgaagta ggtgccgcgg gcgtcgaaga ggcgttgccg gctctcgccg aggaggtgtc
     3661 ccgtcgggcg tgctgcggcg cggagatcac ggacttacgc gacttctcag acacgcccga
     3721 gtctggagcg ggctcctggg cctacgcatt caagaatggc agcagaagga gaacgaaaag
     3781 gccgccgaaa cgaaccgccg gaccgctttg cgcgaggcca gccttgagcg catcgcggaa
     3841 gagctcttcg ctatcgtccg ccatcaggat tcgattccgt atcaacacac cccagtgcat
     3901 gagcacttgg cctgagaggg gacgctgcgt gagcacctgc tccgcatcca gctgacagcc
     3961 ctgcgcttgg acgcggcgat ggacgccatg ctgagcggtc tggaagccgg cctgtctgat
     4021 cctgacgtgg tcgaggccca gcgggccagg gacgtttcct ccgaggctca ccggcacgct
     4081 ttcaccacct ggtatgcatc ggtccccgag ctggcaccgg tcgcggaggg gctctatggg
     4141 caactgcaga cgcctgagca ctgggtgccg tatgtggaca ctgcgtccac gctgacctgc
     4201 ttggcagatc acggcgtggc cctgggcgtg gtcagtgacg tgggttggga tctgcgcaag
     4261 accttcgccc accatggcct ggacggctac ttctccacgt gggtgcattc ctatgaacac
     4321 aacacggaga agccgaaccc gctgctgttc caccacgctt gtcgtgagct gggtgtcgag
     4381 ccagccgaaa ctctcatggt gggtgatcac cccgccaagg acggcggtgc tgtcggcgcg
     4441 ggactgcgct cgtatgtcct gcccgccgga gctgctcctg gctcgcaccg gggactggat
     4501 gccgtgctgc ggctggccgg tttcgactct tccgggctct gaaccaggct gggctggacg
     4561 gccgtcgagc tggcactcgc gagcggtgct tgcggtcagc tggcctgggc ttgttcggca
     4621 agggccttgg tgtgagcgag gcggtaggac ttggtgcccg ggcttggtac tggtgggctc
     4681 gcgtattcaa ctgtcgtgta ttggtccacc ggcagcctcg aacagtgact ttgggtgacg
     4741 gagctgggga gcgttgagtc cgggcaagcg gtgatgtgct ggtggaacga cggcaggcct
     4801 ctgcggcagg tgcgcaacgg ctcgctgggt ggccgctctg taggctgatc gtgcgattcg
     4861 atgaggggag ttcggggtgg agcagccggt ccagcgcggt gcgggcgccg gtgtggaggt
     4921 ggtcgtcctc cccgcgccag gcgctgcggc catgaccagg gaggtcaggg cgccggtaga
     4981 gacgttgtgg cctggaccgg tcaaaccggg gcagccacga ccgtgacggt cagggcggcg
     5041 ctcggccggg ccacccggaa ggtgaaggtc tcctccagat agaggctgac gtcagtgttg
     5101 ctggtggtgg ggaggtagcc gatcgacagg tcctggccga caatgagcag gaagtccccg
     5161 ccgcgctcgc tgagcacgac cgcctgcgtg tctttgcccg tcttctcgtc gaggcagggc
     5221 atcaggacga tctttccgcc gaggatttct gtgaggcgtt cggacagcac ataacagcag
     5281 ctgccggcat ccgcgatgat gcgttggtag cccgtcgtgc cgatggccag cgtgtacggg
     5341 ccgacgacac cactggtccg cagcttgtcc accaccttgg cgatgtcggc ggggtaggtg
     5401 tcggttgcca ccggcagcgt cacgcccgag ggtgatgcct gattaacgat tccggcgatg
     5461 aacgctctgt cccatccgtt gaacaccgcg tagttctcaa agtcggcagc cagtttcgcg
     5521 gccttgtcca gctgggtgag gtcggggttg atgctgccgc gctcgatttc ttcgatggct
     5581 ttgaggggga gggtgaacgg gatgcggacc tgggtcagcg gctgtacgac ccgcagcgcc
     5641 ttgacgttgg tgccagaggg ggtcagcttg tcggggtagg tgttgagttc ggtggctttt
     5701 ccggtgggta cggcgtcttg ctcccagccg ccggggccgg cgaagctcac gacctgtcgt
     5761 gcggcgagca aggggatgag ccggttgcgg gcctcgtcct cgatcacctg ccagacgcgg
     5821 ggggtgatgg gtgccaggcc gcgcagcagg tggttgatgg tgtccatggt gtttatcgct
     5881 ccttcaggtt tccgatggcc agtgacccgt cgccaccggt agatgtggcc gcaccgtcct
     5941 cacgggctga ggtgccgcct gcggagccgg tggccgagcc ggaacccccc gcagcgccgc
     6001 ggcctccgct gccgccgtca ccgccgggac cggccgcgcc tccgctgccg ccgtcaccgc
     6061 cggggccggc cgctccgccg cgcccgccgg taccgaagtt gagggatctg gcccgggcat
     6121 tcccgccgtc gttgccgcct tccgcgccgc cggccccgcc ggtcgcggtg gccgccgcgc
     6181 cggctccggc gtagccacct gctcccgcgt agccgcccgt accgccgtaa ccgccggccc
     6241 cggcgtagcc gcccgtaccg ccgatgccgc cgatacccag cgacccgtcg ccggcaccgc
     6301 ctccgcttcc gccggtgccg ccacctccgg cagtgcctcc ggagccgccg gttcctccgg
     6361 acccggcgtc gccgccgctg ctaccgggcc cgccgtcacc gccggtaccg aagttgacgg
     6421 cctcggcatc ggcgctaccg ccgcggccac cgccttccgc accgccgaca ccgccggtcg
     6481 cggtggccgc cgagccggct ccgccgtagc cgccgacagc gccgtaccca ccggctcccg
     6541 catagccgcc tgccccgcca tagccaccag caccgccata accaccggtg ccggcagcgg
     6601 cggtggcccg ggcacggccg tcggagctga gttccatcgc gagcgttacc tctgcggcct
     6661 ggtccggacg atggggcagg gaaggtccag ccgaacgggg ggaagtgtca tccatcagat
     6721 ctctctaacg ttgcgggcgg ctcgggtggc ccgtggggcc tatgggtcgt gttgtcaggg
     6781 cttccccgcg gtggccgtcc ggctctgcgg gacatcgagg agggtgggca agatcgcccg
     6841 ggtattcgat gaccaccccg atggcctcgg gatcaaccgg atcgctgagg cagccgggct
     6901 gacggtgccg caggtcgagc gagcactggc atggcagagc gagcggttcg ccgcgtggtg
     6961 ccggcggcac tgagcccgaa cgggggtgaa cctgacgtcg gtcactgagc tggcttcgcc
     7021 tggcgttcgg cctcggtggc ctggtatcgg taggactcgg tgcggtctgg atcagcgtgc
     7081 agcggaaagt gatccggtcg ccgattgccg cgcagagcct cgggtcggcg aagatcttgt
     7141 cccattcggc gaacggggag aaccgcccgc cctcgtgctc gaccccgccc ttctcatgtg
     7201 ccccttcctc accaggcccg cagtaaaggg gcgagaacgc ataccaggac ctgaaggcca
     7261 cccagcgcgt cgtctccgtg cgggaacggc cgaacagcac atgtttcacc gcgggcttga
     7321 ggttgtcgta gcggatatgg accgaaggca ccccgcccag cgcccgaaag gcttccacat
     7381 gtccttcgaa gaacgcctcc tgcgaggcgg tggtatagac gcggtggatg gccttgcccg
     7441 agtacgacat ccgcagcgtg aacagcacgc acttgcggcg ctgcccggcc aggtccagcc
     7501 acacgtccgc gaagtcgacc tcggcttcct cgccgggccg cttctcctgc ggcactgtcc
     7561 cctccagatg ccgacgcccc tccttggcct ccaagacgat ctgcgggcgc cgcttgcgca
     7621 cgtagtcacg gatcgtggag taggacgcgt gctcgaaatc gtgctcgcct gccagacgct
     7681 ccaggatccg gtcgacggtg tgcttctgct tcttccaggc ggctccagca cgctcttgcg
     7741 cggcggcggc ttcttccgcc agagggcacc ggacacacca gcgccttcgc cacggtgttc
     7801 cgcgacacca ccttgtaccg ctgcgccagc acccgccccg tgaccgccgg acctaaccgg
     7861 cggtcacgac ggatccgctc aaagatccac tcacgcgacc tgtacaccca cgggctcctg
     7921 acatgaccgt gatcaacgca gcacagcgtg acccggctaa tcagcccaaa gcccccatta
     7981 cgccatcagc ggcatgccac acaccgtcag gcaggctggc tctcaatctc gttgccgaag
     8041 cagtggctct cgttctcaat gccccactgg ctctactcca ccctgtcgaa aacagcagaa
     8101 gggtatgcag gcggaagaag tcgtcgcgga agtctgagcc cgccatcccg acgccggcga
     8161 acagccgaca ggcccgggac gccccaatcg ggtacgggcc tgtcggctgt gacagcgccc
     8221 cggtgcggcg gtcgtgatcc gtcacacccg catcggacca gatcacttga gactgggacc
     8281 aggtcgagtg agacgggaca gcaggaatgg acagcacttc gacagtggtc cagtgtttcc
     8341 tgcgcgtcga tgccccgttc gtgctctgtc tcccagtcga agaacaggcg ggcgtgttcc
     8401 atcgcgcggt tccacgatcc gtagccgtac cttgcgtgat gccgactctc ccactccaca
     8461 cccgaacgcc acctcgtccc gccgcgatgc cccttgggtc agatggtgct cgtcccttct
     8521 gttcatgtcc ggttcagagt gcgggcctgc gacctatggt gatggcgtgg gacatgggga
     8581 gaggagggtc cgtggaaatc gtcgcgatgt cgcagaattt gggtccggga gcttggcggt
     8641 ctcagaacgg cattttcgag gaccgttggc cggcgctcgc ggcgaccatt cagcgggtgc
     8701 agcctgacat tctgatgctg caggaggcgg aaggctggcc ggcggatggt cacgcgcggc
     8761 tgattcgggc cgaagccgac cttgagatgg acggggtgct cgcgcccagt ccgtcggggc
     8821 tgggcccggc gatcttctac cgtcgcgcga cgatggggcg gcgcacctat cagaaccacg
     8881 actactccgc ccacgagaca catcacggct tcgagacgct cggctggaac ctgcccgtcc
     8941 tgcctgccac gctgcccgcg ggcagcgtgc acctgacccc gtacggtggc ccgcgtgccg
     9001 agagccaggt cagcttcatc ggcacccggg tcctgcgtcc tggtgtcggc tacgccatcc
     9061 tcggcggtga cttcaactat gctccggtgg ccggtccccc gccgctggtc ggggccatgc
     9121 gcccgtataa caagggttcc cggctgaagg tcaccgatcc ggagagtccc gggccggacg
     9181 agcctgatcg tgctgtcgcc tggcggatcg cgcgcaaggg tttcgtggac gtcgcctggc
     9241 atctctacaa gcagaccggc gacgaagagc ttctgcggcg caccgccacg gacgaccgga
     9301 tcgactggat cctgatcagc ttcgccctgg cgccgtgcat cgtggactac ggcgtcgtcg
     9361 aggggggctc ggaccacgcc ggggtctggg ctcgtctgga tctgtccctg gccgcgaccg
     9421 acaacgtgtg ggagtacaag taatggctga gccaatggct gcggacgaca tcctgcgggc
     9481 ctgcgggtac ctggaggcgg tgtggatgga ggacgcgacg aacatggccg cgctcctgaa
     9541 tcatcggacc ggggagatac ctacggcgat cctggtcacc gagctgggcg aatccttcat
     9601 gcagcaagcg cttcccgccc agttcggcgt tcacgacgac atgacgcccc aggagttgca
     9661 ggacgcgacc gagaagatga ccgccgatcc gaccgtgcgc gtctccaaag tcctgatcga
     9721 ggcactcaag gagatcgcgc cgaccgcgac gccgccgcag gccgagacca tcgcccgcgc
     9781 tctgatcacc tacctcctcg ccatcagcga cgccacccag gacgacgtga tgccgatgct
     9841 caccgccctg cggcagtccg cgctccagcg ctatggaggc cagggcacgt aaccggtggc
     9901 ggcccgtcat ccacgacgag ccagaccggt ccgtggcctg gcggatcgct gccaccgaat
     9961 caggtcgggg cccggctcgc gagcctgtgc cccggtgtcc ttttatcggt tgattggatg
    10021 ttgaaggcca cgaggcgggg agatgcagat gagcggcggg gcgggcgagg tacggctgac
    10081 gatggggtgt gaggtgcccg tcacccggtg gcagccggcc cttgagacga cgctgatggc
    10141 tccacttcac tgtcccgagt gcctacagtc cacgggcagc gccgagcttc agcgcgtgcg
    10201 gtccgtgcac cctgcccact cacggatctc tccctggtcg tgtagggcgg tgccgctgcc
    10261 ctgccggatg cgtacgctgg cgacatgacg aagcgcgatg agctgcaccg cctggtggac
    10321 cagcttcggc cggagacact ggacgacgcc gcggccgccc tacccgcgtt cagtcggcat
    10381 gctgatcatt tcatgaggca gccccgatgc cggggctgcc tcatgaaatg agtggcctcg
    10441 gagcgcagat cgaggcagta gccgaacacc atgacgcgaa gcggggaccg cggttcccac
    10501 cagggtgccc gacgggaagc ctcggggcag ttggccagcg gggggaccga acccggtgcg
    10561 gcggtggggg atgacgaagc accggcagcg ggggaggggc cgtggtcgga gaacaccgcc
    10621 gcgacatgat cccccaccgt gccgcccgcg ggctctgcat ccggcgtccc cgccgcctgg
    10681 tccgtcccgg ccggggcggt gcgagtcggg ggcggggcgg tgtccaggac acggcagttg
    10741 ggggtggcgg ccgcgcagcg cgcgcacatc tccgcgatcc gccacaacct tccggtacgg
    10801 tccgcgtaca cggtcaggac caccgggccc ccgcaccgca cccgtcggcc gcgcggcccg
    10861 ccgtccg
//
