LOCUS       NZ_CP086322            10405 bp    DNA     linear   CON 26-NOV-2024
DEFINITION  Streptomyces halobius strain 3_2 chromosome, complete genome.
ACCESSION   NZ_CP086322
VERSION     NZ_CP086322.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces halobius
  ORGANISM  Streptomyces halobius
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9239126)
  AUTHORS   Wen,Y., Zhang,W. and Liu,X.G.
  TITLE     Streptomyces nigrumlapis sp.nov.,an antimicrobial producing
            actinobacterium isolated from Black Gobi rocks
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9239126)
  AUTHORS   Zhang,W., Liu,X.G. and Wen,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2021) Northwest Institute of Eco-Environment and
            Resources, CAS, Chinese Academy of Sciences, Donggang West Road 320,
            Lanzhou, Gansu 730000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. FEB-2021
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 241.26X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023277745.1-RS_2024_11_26
            Annotation Date                   :: 11/26/2024 03:43:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,438
            CDSs (total)                      :: 8,346
            Genes (coding)                    :: 7,837
            CDSs (with protein)               :: 7,837
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 509
            CDSs (without protein)            :: 509
            Pseudo Genes (ambiguous residues) :: 0 of 509
            Pseudo Genes (frameshifted)       :: 193 of 509
            Pseudo Genes (incomplete)         :: 418 of 509
            Pseudo Genes (internal stop)      :: 17 of 509
            Pseudo Genes (multiple problems)  :: 113 of 509
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:33:41
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7379075
            Orig. end                         :: 7389480
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086322.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="31"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5405)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            751..1296
                     /locus_tag="K9S39_RS33425"
                     /old_locus_tag="K9S39_33575"
     CDS             751..1296
                     /EC_number="2.3.-.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091834.1"
                     /locus_tag="K9S39_RS33425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_33575"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_248867052.1"
                     /transl_table=11
                     /translation="MHSASGLRVRAATPADLETIAAVHTRARAAYARARVPDAPFDAPA
                     EHARRRAAWAHVLGRADTAGLCAERHGTVVGVASYLACPATCGHRPAGTVTLQQLHVDP
                     GHWGTGVGRALHTACLHAWRAAGHSRAVLDVLWHNHRARAFYAGLGWRPAPDRRPAPDA
                     SHLTLVLPLAPDVPPAGR"
     gene            complement(1247..1927)
                     /locus_tag="K9S39_RS33430"
                     /old_locus_tag="K9S39_33580"
     CDS             complement(1247..1927)
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /GO_function="GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA]"
                     /GO_process="GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536870.1"
                     /locus_tag="K9S39_RS33430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_33580"
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="WP_248867053.1"
                     /transl_table=11
                     /translation="MTSPQDLLAFWETTGSAKTFTHPLDTELLDGYLPRTARVLDYGCG
                     YGRLTAELAGLGYRSLLGVDVSAALIERGRREHPGLDLMRWVDFPLPFDDAEFDAALLF
                     AVLTCVPQDAAQSAIVAELGRLVRPGGVLYLSDVPLQYDAYHKERYGRWREECGIYGVF
                     TTPDGGVFRHHAPERLRGLVREAGFSVLRERVGSVATLNGHTAKRLQIIARREGRPGPG
                     AAPA"
     gene            complement(1997..2320)
                     /locus_tag="K9S39_RS33435"
                     /old_locus_tag="K9S39_33585"
     CDS             complement(1997..2320)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016642863.1"
                     /locus_tag="K9S39_RS33435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_33585"
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_248867054.1"
                     /transl_table=11
                     /translation="MSIPAESLTNPVVRAFVTALNAHDRSGFQAALAPDATMSDDGTDR
                     DLRQWTEQEIFSSNGHMEVESETDDGLSLLARYSNDTWGDMRTRWRFTVQDGKVIRFET
                     RQA"
     gene            2699..3781
                     /locus_tag="K9S39_RS33440"
                     /old_locus_tag="K9S39_33590"
     CDS             2699..3781
                     /NRPS_PKS="Domain: Aminotran_5 (19-354). E-value: 5.9e-20.
                     Score: 63.4. Matches aSDomain:
                     nrpspksdomains_K9S39_RS33440_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448151.1"
                     /locus_tag="K9S39_RS33440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_33590"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_248867055.1"
                     /sec_met_domain="Aminotran_5 (E-value: 6.4e-13, bitscore:
                     47.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQRPEPCASLGGSEFAPETTYLNSASSGLLPARTAAALRAALDES
                     TSYGTMGRDYFGPAAASRAAFARLLDVPEERVALGSSVAVHSAFVAGTLPPGAEVLVPE
                     GDFSSLVNPFAARPGIKLRTAPLADLAEAVRPGTALVAFSAVQSRDGRIADMAAVREAA
                     RAHGARTFLDVTQAAGWLPLSVAGFDYVTCGAYKWLLCPRGATFMAFGGDAAEGGAGAW
                     PDPLHAGWVAGEDFDESCYGPIRRPATTARRFDEPHAYYAYVGAQHSLTLIDEIGIAAI
                     HAHNTALADHYRAGLAGLGRTLVPAPGSAIVSTPGLAGAESRLAEAGVRVSVRDGQLRA
                     AFHLYNSTDDVDRLLGLLRR"
     aSDomain        2756..3760
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_K9S39_RS33440_Aminotran_5.1"
                     /evalue="5.90E-20"
                     /label="K9S39_RS33440_Aminotran_5.1"
                     /locus_tag="K9S39_RS33440"
                     /protein_end="354"
                     /protein_start="19"
                     /score="63.4"
                     /tool="antismash"
                     /translation="TYLNSASSGLLPARTAAALRAALDESTSYGTMGRDYFGPAAASRA
                     AFARLLDVPEERVALGSSVAVHSAFVAGTLPPGAEVLVPEGDFSSLVNPFAARPGIKLR
                     TAPLADLAEAVRPGTALVAFSAVQSRDGRIADMAAVREAARAHGARTFLDVTQAAGWLP
                     LSVAGFDYVTCGAYKWLLCPRGATFMAFGGDAAEGGAGAWPDPLHAGWVAGEDFDESCY
                     GPIRRPATTARRFDEPHAYYAYVGAQHSLTLIDEIGIAAIHAHNTALADHYRAGLAGLG
                     RTLVPAPGSAIVSTPGLAGAESRLAEAGVRVSVRDGQLRAAFHLYNSTDDVDRL"
     gene            complement(3989..4888)
                     /gene="thpD"
                     /locus_tag="K9S39_RS33445"
                     /old_locus_tag="K9S39_33595"
     CDS             complement(3989..4888)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606339.1"
                     /locus_tag="K9S39_RS33445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_33595"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_248867056.1"
                     /sec_met_domain="PhyH (E-value: 4.9e-38, bitscore: 130.4,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGTAEVITPRKDPVVWSQPGAAGPFEPSELS
                     DFERDGFFAIPELLTAEEVAVYRAELDRLVLDPAMRANPRSIVEPKSQSVRSVFEVHKI
                     SEVFAKLVADPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTVSVSIALTENYDTNGGLMIMPGSHRHFVGCEGETPKDNYKRSLQMQDAGIPSDKI
                     LTNMADEHGIRLFTGKAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVENTAVEPFA
                     APVRRPEYIGARDFTPVK"
     gene            complement(5001..5405)
                     /locus_tag="K9S39_RS33450"
                     /old_locus_tag="K9S39_33600"
     CDS             complement(5001..5405)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358304.1"
                     /locus_tag="K9S39_RS33450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_33600"
                     /product="ectoine synthase"
                     /protein_id="WP_248867057.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.6e-52, bitscore:
                     174.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKSQSGTWESKRIVLAKEGVGFSLHETTLY
                     AGTETSMWYANHYEAVLCVEGEAELTNDETGEKFTITPGTMYLLDGHEKHTMRIKQDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEPELG"
     gene            complement(5452..6720)
                     /gene="ectB"
                     /locus_tag="K9S39_RS33455"
                     /old_locus_tag="K9S39_33605"
     CDS             complement(5452..6720)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 4e-73.
                     Score: 238.3. Matches aSDomain:
                     nrpspksdomains_K9S39_RS33455_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="K9S39_RS33455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_33605"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_248867058.1"
                     /sec_met_domain="Aminotran_3 (E-value: 8.5e-77, bitscore:
                     257.4, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRGWPTVFDRAQGSRMTDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGVTHGLDMSTTAKRAFLESFQNIILRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYLDGQIPDFLWFEKLLEDQGSGLNQPAAVIVETVQGEGGINVARA
                     DWLRALSDLCKRRDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPLA
                     LTLFKPELDVWEAGEHNGTFRGNNPAFVTAAAALDTYWADGQMEKQTLARGEIVEEHLK
                     AIVEEHPGAFTEYRGRGLVWGLECVDKSLANKIAKRAFELGLLIETSGPESEVVKLLPA
                     LTTTPEELDEGLRILARATRDCA"
     aSDomain        complement(5626..6636)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_K9S39_RS33455_Aminotran_3.1"
                     /evalue="4.00E-73"
                     /label="K9S39_RS33455_Aminotran_3.1"
                     /locus_tag="K9S39_RS33455"
                     /protein_end="365"
                     /protein_start="28"
                     /score="238.3"
                     /tool="antismash"
                     /translation="FDRAQGSRMTDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGVTHGLDMSTTAKRAFLESFQNIILRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYLDGQIPDFLWFEK
                     LLEDQGSGLNQPAAVIVETVQGEGGINVARADWLRALSDLCKRRDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPLALTLFKPELDVWEAGEHNGTFRGNNPAFV
                     TAAAALDTYWADGQMEKQTLARGEIVEEHLKAIVEEHPGAFTEYRGRGLVWGLECV"
     gene            complement(6848..7414)
                     /gene="ectA"
                     /locus_tag="K9S39_RS33460"
                     /old_locus_tag="K9S39_33610"
     CDS             complement(6848..7414)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536877.1"
                     /locus_tag="K9S39_RS33460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_33610"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_248867059.1"
                     /transl_table=11
                     /translation="MTAAQADHARARTDIRELPEGFKLDTPRVEDGAAIWRIARDSKAL
                     DLNSSYSYLLWCRDFAATSVVARDTEGEPAAFITGYIRPDRPETLVVWQVAVDDAHRGQ
                     GLAAALLDGLTTRATHELGIRFVETTITPDNAASNQLFASFAERHGVALGREVLFDAGL
                     FPEQGHEPEVLHLIGPFDVPAGTGQ"
     misc_feature    7414..7428
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.41"
                     /tool="antismash"
     misc_feature    7570..7585
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.52"
                     /tool="antismash"
     misc_feature    7580..7595
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7580..7597)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.23"
                     /tool="antismash"
     gene            7930..9021
                     /locus_tag="K9S39_RS33465"
                     /old_locus_tag="K9S39_33615"
     CDS             7930..9021
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606343.1"
                     /locus_tag="K9S39_RS33465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_33615"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_248869077.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 3.2e-16, bitscore:
                     58.4, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEGAVLHLKGRVLLGPDDVRDELWVVGGRVTYERPAGAREVTTV
                     QGWALPGLVDAHCHVGLDAHGPVDDATSEKQALTDRDAGTLLIRDAGSPSDTRWIDDRE
                     DLPRIIRAGRHIARTKRYIRNYAHEIEPEDLVAYVAQEARRGDGWVKLVGDWLDRSTGD
                     LGFCWPRGAVEAAIAEAHRLGARLTAHCFAEESLGPLVEAGIDCVEHATGLTEDTIPLF
                     AERGVAIVPTLVNIATFPRLADSGETKFPKWADHMRRLHARRYDTIGAAYDAGVPIYAG
                     TDAGGSLAHGLVAQEVAELMKAGLPVEAALSATTWGARDWLGRPGLTEGAPADLVVYES
                     DPRADVRVLAAPRRVVLRGRVVG"
     misc_feature    complement(9159..9172)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.7"
                     /tool="antismash"
     misc_feature    complement(10045..10057)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.46"
                     /tool="antismash"
ORIGIN
        1 tccgcacccg cgccgaactc cccgacgtcc aggcgccggt gaccccgggc gcgggcggcg
       61 aacacaccgt cagcgcgggc ttcctcaccg tccccgccgc tcgcctcgcc accgaagggc
      121 cgcacgacct cctcctggag gagtgcttcg gcccggtgac cgtcctcgcc cgctacaccg
      181 acgagtccga gatcggcgcc gtactcgccc ggctgtccgg gaatctcacc gcgacgctcc
      241 atctgggcga cgcggagagc gcggggaccg atgctgccgt gggtacggag agcgccgcga
      301 ccggtgatgt ggcaggcgac gcgggtgacg ctggggatgg tggtgacggc gtcggcctgg
      361 gcgcccgtgg cgctgcggac agcaacgacg gcatcggcct gggtgcccga ctgctctccg
      421 cgctcacccc gctggccgga cgcatcgtgg tcaacggctg gccgaccggt gtcgcggtag
      481 cccccgccca gcaccacggc ggcccctacc cggccaccac ctccacctcg acctccgtcg
      541 gcggcaccgc cgtcgaacgc tggctccgcc ctgtcgtcta ccaggacacc ccgcccgccc
      601 tgctcccgcc cgagctccgt gaggacaacc ctccccctgg gggcacctcc cggcgtcagc
      661 cgggggactt cggccggggg gacccccatc tcgcttcgct cggcctgcca cgtcgtgtcg
      721 acggccgtgc cgagggcgcg tagcggcacc gtgcactccg cctccggcct ccgggtccgt
      781 gccgccaccc ccgccgacct ggagacgatt gccgccgtgc acacccgcgc ccgcgcggcg
      841 tacgcccgtg cacgggtccc ggacgccccc ttcgacgcgc cggccgaaca cgcccgccgg
      901 cgcgccgcct gggcacacgt cctgggccgg gccgacaccg ccggcctctg cgccgaacgc
      961 catggcacgg tcgtcggcgt cgcctcctac ctcgcgtgcc cggccacctg cggccaccgg
     1021 ccggcgggca ccgtcaccct gcagcagctg catgtcgacc ccggccactg gggcaccggc
     1081 gtcggccgtg cactgcacac cgcctgcctg cacgcctggc gcgcggccgg ccactcccgt
     1141 gccgtcctcg acgtgctctg gcacaaccac cgtgcccgcg ccttctacgc cggcctcggc
     1201 tggcgccccg ccccggaccg ccgcccggcc cccgatgcca gccacctcac gctggtgctg
     1261 cccctggccc cggacgtccc tcccgccggg cgatgatctg cagccgtttc gcggtgtgcc
     1321 cgttcagcgt cgcgaccgac cccacccgct ctcgcagcac cgagaacccc gcctcgcgca
     1381 ccagtccccg cagccgctcc ggcgcatgat ggcggaacac cccgccatcg ggcgtcgtga
     1441 acaccccata gatgccgcac tcttcccgcc accgcccgta ccgctccttg tggtacgcgt
     1501 cgtactgcag cggcacatcg ctcagataca gcaccccacc cggccgtacc agccgcccca
     1561 gctccgcgac gatcgccgac tgcgccgcgt cctgcggaac gcacgtcagc accgcgaaca
     1621 gcagcgccgc gtcaaactcc gcgtcgtcga acggcaacgg gaagtccacc cagcgcatca
     1681 gatccagccc ggggtgttcg cgccgcccgc gctcgatgag tgccgccgag acatccaccc
     1741 cgagcaacga ccgataaccg agcccggcca gctcggccgt cagccgtccg taaccgcagc
     1801 cgtagtccag cacccgggcg gtacgcggca gatagccgtc cagcaattcc gtatcgagcg
     1861 gatgggtgaa ggtcttggcg gaaccggtcg tttcccagaa agcgagaaga tcctgaggtg
     1921 aggtcataac gggaccgtac gtccttgccg ttatgtctgt cctgctctgc gcctgtctcg
     1981 tctgcgcctg tcctgtctac gcctgtctcg tctcgaaacg gattaccttg ccgtcctgga
     2041 cggtgaaccg ccagcgggtg cgcatatcgc cccaggtgtc attgctgtag cgggccagca
     2101 gtgaaagccc gtcatcggtc tccgactcca cctccatatg gccgttcgag gagaagatct
     2161 cctgctcggt ccactgcctc agatcgcggt cggtaccgtc gtcggacatc gtcgcatccg
     2221 gtgccagcgc ggcctggaaa ccgctgcggt catgggcgtt gagcgcggtg acgaaggcgc
     2281 gtacaacggg gttggtcagg ctttcggcgg gaatgctcac gaccattcct tcaattcggg
     2341 gagcgttcgg ggaggctttc gcgaagggtt cgggaggctt tccggaatcg ttcgggagga
     2401 tcccgggaat ccccctggaa gaatcttgga gaatgttcgg gaagcgggaa gcgggaagcg
     2461 ggaagcggga agcgcgggac agggaatccc cggcaggata atttccggag ggctcccgcc
     2521 gggcaccggc ggcgtgtcga gcgtgccgcg gcgctgctga cgtcgcttcc ccgccgggtg
     2581 ttgagggagc gttgccccgt ccgcgtcccc cgctgacctg cgcggataag ggatgcttgc
     2641 ggaacggcga agagaatttg tcatagacgg ggagccggac gccgagcaga gtgggggcat
     2701 gcagcgacct gagccatgtg cctcactcgg cgggagcgaa ttcgctcccg agacgaccta
     2761 tctgaattcc gcctccagcg gactcctccc ggcccgcacc gccgctgccc tgcgcgcggc
     2821 cctcgacgaa tccacctcct acggcaccat gggccgcgac tacttcggtc ccgccgcggc
     2881 ctcccgggcc gccttcgccc ggctgttgga cgtccccgag gagcgggtcg ccctcggcag
     2941 ctcggtcgcg gtgcactccg cgttcgtcgc cggcacgctc ccaccgggcg ccgaagtgct
     3001 ggtcccggag ggtgacttca gctctctggt caatccgttc gccgcgcgcc ccggcatcaa
     3061 gctgcgcacc gcgccgctgg cagacctcgc cgaggcggtg cgtcccggca ccgcgctggt
     3121 cgcgttcagc gccgtgcagt cgagggacgg ccggatcgcc gacatggccg cggtacggga
     3181 ggccgcgcgg gcccatgggg cgcgtacctt cctggacgtc acgcaggcag ccggctggct
     3241 gccgttgagc gtcgccggct tcgactacgt cacctgcggg gcctacaagt ggctgctgtg
     3301 cccgcgcggc gccaccttca tggcgttcgg cggggacgcg gccgagggcg gcgccggggc
     3361 ctggccggac ccgctgcacg cgggctgggt ggccggtgag gacttcgacg agtcctgcta
     3421 cggcccgatc cgccggccgg ccaccaccgc ccggcgcttc gatgaaccgc acgcctacta
     3481 cgcgtacgtc ggcgcccagc actccctcac cctcatcgac gagatcggca tcgccgccat
     3541 ccacgcccac aacaccgcgc tcgccgacca ctaccgggcc ggtctggccg ggctcggccg
     3601 caccctcgtc ccggcacccg gttcggcgat cgtgtccacc ccgggactcg ccggcgcgga
     3661 gagccggctg gccgaggcgg gcgtacgggt ctcggtgcgc gacgggcagc tgcgcgcggc
     3721 cttccacctc tacaactcca cggatgacgt ggaccggctg ctgggactgc tgcgccggta
     3781 gggaatgaag ttcccctgag ctccgcgcga ccggcgctgc ttctgccggt gtcgtggcgt
     3841 ctcgttccgt gccgtgctgg aaggggcttc tggtaggggg ccgccatgcc gtacgtccgc
     3901 ctcgcggcgc ggccgccgca cagacccact gtgcggcggc cgcgccaccg gtgcttccgc
     3961 atgccctcag gccacggggt tcgtcgccct acttcacggg ggtgaagtcc cgcgcgccga
     4021 tgtactccgg ccgccggacc ggcgccgcga acggctccac cgccgtgttc tccacgctgt
     4081 tgaacacgat gaagacattg ctgcgcgggt acggcgtgat gttgtcaccg gagccgtgca
     4141 tcgcgttgca gtcgaaccag gtcgccgagc cggccttgcc ggtgaacagg cggatgccgt
     4201 gctcgtccgc catgttcgtc aggatcttgt ccgacgggat gcccgcgtcc tgcatctgca
     4261 gcgaccgctt gtagttgtcc ttcggcgtct caccctcgca gcccacgaag tgccggtgcg
     4321 acccgggcat gatcatcagc ccgccgttgg tgtcgtagtt ctccgtcagc gcgatcgaga
     4381 ccgacacggt gcgcatgttc ggcagaccgt cctcggcgtg ccaggtctcg aagtcggagt
     4441 gccagtagaa gcccgaggca ccgaaaccgg gcttcacatt gatccgtgac tggtggacat
     4501 agacgtccga gcccaggatc tgccgggcgc ggccgaccac ccgcggatcg gcgaccagct
     4561 tggcgaacac ctcgctgatc ttgtgcacct cgaagacgga ccggacgctc tgcgacttcg
     4621 gctcgacgat ggagcgcggg ttggcgcgca tcgccgggtc gagaacgagc cggtccagtt
     4681 cggcgcggta caccgcgact tcttccgccg tgagcagttc cgggatggcg aaaaagccgt
     4741 cgcgttcgaa gtcgctcagc tcggacggct cgaaggggcc ggccgctccg ggctgcgacc
     4801 acaccaccgg gtccttccgc ggggtgatca cctcagcggt cccacgggtc gggtacaggt
     4861 cggcggtgcg ctcgggtgcg gtggtcatgg tgttgccttc ctctcctctc gtacgtgctc
     4921 ttcttacctg cgcgtgatgc gtgccttcct cagctgccgg acggggcagc cgactgccgg
     4981 acggggcaga cggatgcggc tcagcccagt tcgggctcgg tgagcaacgg gtagaccccg
     5041 ttctcgtcgt ggtcctcacg gccggtgacc ggagggttga acacacacac gcagcggaag
     5101 tcctgcttga tccgcatggt gtgcttctca tgcccgtcga gcaggtacat cgtgcccggc
     5161 gtaatggtga atttctcgcc ggtctcgtcg ttggtgagct cggcctcacc ctcgacgcac
     5221 aggacggcct cgtagtggtt ggcgtaccac atcgaggtct cggtccccgc gtacagggtg
     5281 gtctcgtgca gggagaagcc gacgccctcc ttggcgagca cgatgcgctt gctctcccat
     5341 gtgccggact gggacttgac gtggcggtcg gtgccctcga tgtccttgaa cgagcggacg
     5401 atcacggtga gtcgttgcct ttctgtgaaa cggtcgggaa ctccgcgccg gtcaggcgca
     5461 gtcgcgggtc gcgcgggcga ggatccgcag accctcgtcc agttcctcgg gggtggtcgt
     5521 cagcgccggg agcagcttga cgacctcgct ctccgggccg gaggtctcga tcagcagacc
     5581 cagctcgaag gcgcgcttgg cgatcttgtt ggcgaggctc ttgtccacgc actccaggcc
     5641 ccacaccaga ccgcggccgc ggtactcggt gaaggccccg gggtgctcct cgacgatggc
     5701 cttgaggtgc tcctcgacga tctcaccccg ggccagggtc tgcttctcca tctggccgtc
     5761 ggcccagtac gtgtccagcg cggcggcggc cgtgacgaag gccgggttgt tgccccggaa
     5821 ggtgccgttg tgctcgcccg cctcccagac gtccagctcc ggcttgaaca gggtgagcgc
     5881 gagcggcagg ccgtagccgc tgatggactt cgagacggtg acgatgtccg gcacgatgcc
     5941 cgcctcctcg aaggagaaga aggcacccgt tcggccgcaa cccatctgga tgtcgtccac
     6001 gatcagcagc atgtcgcggc gcttgcacag atcggacagg gcgcgcagcc agtcggcgcg
     6061 ggcgacgttg atgccgccct cgccctggac cgtctcgacg atcaccgcgg cgggctggtt
     6121 gaggcccgag ccctggtcct ccagcagctt ctcgaaccac aggaagtccg ggatctggcc
     6181 gtcgaggtag ttgtcgaacg gcatcggggt gccgtgcacc agcgggatgc ccgcgccggc
     6241 ccgcttgaag gcgttgccgg tcacggccag cgagccgagc gacatgccgt ggaaggcgtt
     6301 ggtgaaggac acgatcgact cgcggccctt gaccttacgg gccagcttca gcgcggcctc
     6361 gacggcgttg gtgcccgtcg ggcccgggaa catgaccttg tagggcaggt cacgcggccg
     6421 caggatgatg ttctggaagg actccaggaa cgcccgcttg gccgtggtgg acatgtccag
     6481 gccgtgggtg acgccgtccc gctcgatgta gtcgatcagg gcgcgtttca gcaccgggtt
     6541 gttgtggccg tagttgagcg agccggcgcc ggcgaagaag tcgaggtacg tgtggccgtc
     6601 ctcgtcggtc atacggctgc cctgcgcgcg gtcgaagacg gtgggccagc cacggcagta
     6661 gctgcgcacc tccgactcca cggtctcgaa gacgctgagg tcgggctggg tgatagtcac
     6721 agcatgctcc agggagatga gtgcgaaaga ggggggaaga ctgagggggc cgatcgatgc
     6781 cggccgcgtg gggtgcgggg acccgtgggt gcgcatgcgg cagtacggcc gcggggcacg
     6841 cgggacgtca ctgcccggta ccggccggta cgtcgaacgg gccgatgagg tgcaggacct
     6901 ccggctcgtg gccctgttcc gggaacagtc ccgcgtcgaa gaggacctca cggcccagtg
     6961 ccactccgtg gcgctcggcg aacgacgcga acagctggtt cgaggcggcg ttgtcgggcg
     7021 tgatggtggt ctcgacgaat ctgatgccca gctcatgcgt ggcgcgggtg gtcagcccgt
     7081 ccaggagtgc ggccgccagc ccctgaccac ggtgcgcgtc gtcgacggcg acctgccaga
     7141 cgacgagtgt ctccgggcgg tcggggcgga tgtagccggt gatgaaggcg gccggctcgc
     7201 cctccgtgtc gcgggcgacg acggaggtgg cggcgaagtc gcgacaccac aagaggtagc
     7261 tgtaggagga gttgaggtcc agcgccttgg agtcgcgggc gatgcgccag atcgcggctc
     7321 cgtcctccac gcgtggggtg tcgagcttga agccctccgg caattctctg atatcggtgc
     7381 gggcacgtgc atggtctgct tgtgcggcgg tcatggaaat caaatttacc cagggaaaaa
     7441 cgaaattgca tcgcgggcag gggttacgcc gacggcttgg ttgtgttatc gcgcgggggc
     7501 gcccgagcgc gcggatccat cgcgggatgt cccgatttga ccaggagatt cggggtgaaa
     7561 cggacacatt gtgtagtcgg tcacagctcc gtaacgtcat ggagacgcgt ccgaattacg
     7621 gtacttgacg ctagcgaaat cttggcgttt gaggtgcgga aaaccgggca gaagaatacg
     7681 ggaagctgtg cttctgaaga attgggaatt cgcaagtgga aggtctcccg gaaggcatcc
     7741 gggagagaaa gcggggcggc cgcgggggtt tccgcgaaac gtccgggata acgcccggga
     7801 agccgcagaa gcggcccacg gacggcgcgg aaccagcccc cgggcgctcg gtggaccgct
     7861 gggggacggc cgggagaagg ccctctccgc ccgccgcggc acccggcata cagtgcctgc
     7921 atgacgtcca tgagtgaggg tgcggtgctg cacctcaagg ggagggtcct actcgggccg
     7981 gacgacgtac gcgacgagct gtgggtcgtc ggcggtcggg tgacgtatga gcggccggcc
     8041 ggggcgcggg aggtcaccac cgtccagggc tgggcgctgc cgggcctggt cgacgcccac
     8101 tgccacgtgg ggctggacgc ccacggcccg gtggacgacg ccaccagcga gaaacaggcg
     8161 ctgaccgacc gcgacgcggg caccctgctg atccgggacg ccggatcgcc ctcggacacc
     8221 cgctggatcg acgaccgcga ggatctgccg aggatcatcc gcgccggccg gcacatcgcc
     8281 cgtaccaagc gctacatccg taactacgcg cacgagatcg agccggagga cctggtcgcc
     8341 tatgtcgccc aggaggcccg gcgcggcgac gggtgggtca agctcgtcgg tgactggctc
     8401 gaccgctcca ccggcgatct ggggttctgc tggccgcgcg gcgcggtcga ggcggccatc
     8461 gccgaggccc accggctggg cgcccggctc accgcccact gcttcgcgga ggaatccctg
     8521 ggcccgctcg tcgaagccgg catcgactgc gtcgagcacg ccaccggcct caccgaggac
     8581 accatcccgc tgttcgccga gcgcggggtc gcgatcgtcc ccacgctggt caatatcgcc
     8641 acgttcccgc gcctggccga ctccggcgag acgaagttcc cgaagtgggc ggaccatatg
     8701 cggcgactgc acgcacgccg ctacgacacc atcggggcgg cctacgacgc cggggtgccc
     8761 atctacgccg gtacggacgc cggcggctcg ctcgcgcacg gcctggtcgc ccaggaggtc
     8821 gccgagctga tgaaggccgg cctccccgtc gaggccgcgc tctccgcgac cacctggggt
     8881 gcccgggact ggctcggccg ccccggcctc accgagggtg cccccgccga cctggtggtc
     8941 tacgagtccg atccgcgcgc ggacgtccgg gtgctggccg ccccgcgccg ggtggtcctg
     9001 cgcggccggg tggtcggctg acggggcgtc atgcgtctcg tcaccgcccg cgtcccgtca
     9061 cccgtcccgt caacacatgt gtcccgcacg gtggtacggc tgtgactgag ggaaagtctc
     9121 ctgccacagg agtcgattca agccacaaca ccaactcata agaatgaatt taccttgtga
     9181 ggatgcgcgt tcccccaaaa ggtagacacg atgtgcgcgt caaggctgtt cggcccgcgc
     9241 gatgtccccc attggcgcgc cgacggctcc gtttctctcg taagggtccc actcacgtgt
     9301 ccaccagctc cagccattcc tccggcacgc ccacgcgccg tgcctcccgc cggctcgccg
     9361 ccacctgggt cgccgcctcc ctcacggccg gtcccgccgt actgctcggc gccgctccgg
     9421 cgcacgccac cggcgaccac ggcacctccg gcgcggccat cctgcgcgcc gacctcaacg
     9481 tcggtctgct ccacaagacg gccgaagtcc cgctgaaggc cacgctcaac gaggtgcacg
     9541 caccggcctc ggccgagaag accgcgctct ccgtccagct ggacggcgtc aaccacggca
     9601 agtcgttcaa catgctgcgc gcgaacgtcg ccaccgcgcg tgccactgcc gacggccaca
     9661 agaccgaggg ctacgccaac ctggagcacg ccgaggtgca tctcccggcc cttgcgtcgc
     9721 agtccctgct cgaggtcaag aaggtcacct ccaaggcgat atgcacggcg ggcgagcgcc
     9781 cgcgggccga gtcgaacatc ctgggcaccg tccgcgtcct gggcaaggag gtcaccctcc
     9841 gcgccgacgg gacgaaacag gtcaaggtgc ccggtgtggg cgaagtacga ctcgacctgt
     9901 ccaagaagag cgtcacctcc aagagcgccg cggccaccgc gctcgatctg aacgtctccg
     9961 tgaacccgct caagctcaac gtcgcggacg tcaagggaca catcaagctc gccgaggcca
    10021 gctgcaccac cggcaaggga tccggtgacg gcggcacgtc cggcggcagc ggcggccaga
    10081 cgggcggcgg cagcggcggt cagaccggcg gcggcagcgg cggtcagacc ggcggcggca
    10141 gcagcggcgg cagcggtggt caggcgggcg gcggcagcgg cggtcagacc ggcggcggca
    10201 gcggcggcag cggcggtcac tcgtccggcg gccagaccgg cggcagctcc gccgggcagg
    10261 cctccggcgg ggcgtcgggc ggcggcaacg acacccatcc gcagaccggc accaagcccg
    10321 ccggcgacaa cctcgccgag accggcagca gttcggccac cccgtacctc gccggtggcg
    10381 cggccctgct gctcttcgcg ggcgc
//
