LOCUS       NZ_CP086322            11312 bp    DNA     linear   CON 26-NOV-2024
DEFINITION  Streptomyces halobius strain 3_2 chromosome, complete genome.
ACCESSION   NZ_CP086322
VERSION     NZ_CP086322.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces halobius
  ORGANISM  Streptomyces halobius
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9239126)
  AUTHORS   Wen,Y., Zhang,W. and Liu,X.G.
  TITLE     Streptomyces nigrumlapis sp.nov.,an antimicrobial producing
            actinobacterium isolated from Black Gobi rocks
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9239126)
  AUTHORS   Zhang,W., Liu,X.G. and Wen,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2021) Northwest Institute of Eco-Environment and
            Resources, CAS, Chinese Academy of Sciences, Donggang West Road 320,
            Lanzhou, Gansu 730000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. FEB-2021
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 241.26X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023277745.1-RS_2024_11_26
            Annotation Date                   :: 11/26/2024 03:43:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,438
            CDSs (total)                      :: 8,346
            Genes (coding)                    :: 7,837
            CDSs (with protein)               :: 7,837
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 509
            CDSs (without protein)            :: 509
            Pseudo Genes (ambiguous residues) :: 0 of 509
            Pseudo Genes (frameshifted)       :: 193 of 509
            Pseudo Genes (incomplete)         :: 418 of 509
            Pseudo Genes (internal stop)      :: 17 of 509
            Pseudo Genes (multiple problems)  :: 113 of 509
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:33:41
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2696044
            Orig. end                         :: 2707356
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086322.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11312
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="21"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11312
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11312
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6312](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6312
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(327..599)
                     /locus_tag="K9S39_RS12470"
                     /old_locus_tag="K9S39_12560"
     CDS             complement(327..599)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K9S39_RS12470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="K9S39_12560"
                     /product="hypothetical protein"
                     /protein_id="WP_248863410.1"
                     /transl_table=11
                     /translation="MYSQESISGRRRGRTEPTAEMLSGLACLICGTDYRSAPAPEAVVV
                     SHRDDRRLLACHGTCARMASGSVNGLDETPLPLAERVRRHQADHS"
     gene            1123..1311
                     /locus_tag="K9S39_RS12475"
                     /old_locus_tag="K9S39_12565"
     CDS             1123..1311
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA]"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1255: cold-shock
                     DNA-binding domain protein"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008746266.1"
                     /locus_tag="K9S39_RS12475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_12565"
                     /product="cold-shock protein"
                     /protein_id="WP_248863411.1"
                     /transl_table=11
                     /translation="MRWFNPDKGFGFIAPDEGGAGIFVHHSAIDMGGFRSLEENQRVEF
                     TASQGPKGMQADQVSAL"
     gene            1485..2525
                     /locus_tag="K9S39_RS12480"
                     /old_locus_tag="K9S39_12570"
     CDS             1485..2525
                     /EC_number="1.14.19.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) delta12_cd03507"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381819.1"
                     /locus_tag="K9S39_RS12480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_12570"
                     /product="acyl-CoA desaturase"
                     /protein_id="WP_248868710.1"
                     /sec_met_domain="delta12_cd03507 (E-value: 1.7e-18,
                     bitscore: 65.6, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKPPPAGRGSDYAALSREVKQSGLLKRRPGYYSVKVGTNLLLLA
                     AGWTAFALIGQSWWQLLTAAFLAVMFTQTGFIGHDAGHHQIAASKRVNNLIGHVHADLL
                     IGLSYSWWISKHNRHHSHPNHVDRDPDIADGAIAFTQDHARVRRGPYAWLARHQAWLFF
                     PMLLLEGLALHVAGVRALLDRTGTATSRRTKLAEAGLLTAHLGGYLAALFLVLSPVQAV
                     CFLLVHQGLFGLYMGCSFAPNHKGMPIFAKDDKIDFLRRQVLTSRNIRGHRFTDFALGG
                     LNYQIEHHLFPSMPRPSLRHAQELVRTFCVRRGIAYHETGLFNSYAQVLRHLHAVGGPL
                     RPELEY"
     gene            complement(2566..2901)
                     /locus_tag="K9S39_RS12485"
                     /old_locus_tag="K9S39_12575"
     CDS             complement(2566..2901)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K9S39_RS12485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="K9S39_12575"
                     /product="hypothetical protein"
                     /protein_id="WP_248863412.1"
                     /transl_table=11
                     /translation="MRGHHSVHNLAEHIAARSPGYGPVTNLVTGQAEMLDQIDPAWGRR
                     FRSGGMDGTQTAQPCGHDPLERLAWIAGAWQGQDPYTNLAFFRGDDISVEQIAQFHGAD
                     PQEFSRG"
     misc_feature    2951..2962
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.82"
                     /tool="antismash"
     gene            2989..3189
                     /locus_tag="K9S39_RS12490"
                     /old_locus_tag="K9S39_12580"
     CDS             2989..3189
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K9S39_RS12490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="K9S39_12580"
                     /product="hypothetical protein"
                     /protein_id="WP_248869237.1"
                     /transl_table=11
                     /translation="MPSWTTPRRLAAAVVLCAVFASGWFLGQPVYVPECTIGPHRYSDH
                     LEAENCDTRPRLLAWLNGDWS"
     gene            complement(3206..3328)
                     /locus_tag="K9S39_RS42070"
     CDS             complement(3206..3328)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K9S39_RS42070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_283112307.1"
                     /transl_table=11
                     /translation="MYALAALILLATVVLFTLTVLALTGELWHTNGTGPLNEGH"
     gene            complement(3494..3763)
                     /locus_tag="K9S39_RS12495"
                     /old_locus_tag="K9S39_12585"
     CDS             complement(3494..3763)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015660943.1"
                     /locus_tag="K9S39_RS12495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_12585"
                     /product="hypothetical protein"
                     /protein_id="WP_248863413.1"
                     /transl_table=11
                     /translation="MTERQGDIPDEFDIKWAEQGPKEPSARARMLAARWKDNPPGPQPF
                     RADPDPGVRPARFTDSGYRRSWASVAIVAAGLVLVLAAFALLTG"
     gene            3848..4489
                     /locus_tag="K9S39_RS12500"
                     /old_locus_tag="K9S39_12590"
     CDS             3848..4489
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF022655.5"
                     /locus_tag="K9S39_RS12500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_12590"
                     /product="DUF2993 domain-containing protein"
                     /protein_id="WP_248863414.1"
                     /transl_table=11
                     /translation="MRMRMPLILLGSLVVLAVGADLAAEQIAENIAANKFGERLPGQQG
                     SAEVDIKGFPFLTQAATGTFDEISVTVKDTAAPAGSSTVTLPRATLTARGVKVEGTSSA
                     VADLVEGQATVPYSALATALEQQNPSGGPVEIAPVTGGAARVRITQGGRDLIARLSGQG
                     EDIAFTPEVEGAPPQAVAVATGPLRADITGATPDEGGVRLHFAGRLVSLG"
     misc_feature    complement(3851..3864)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.33"
                     /tool="antismash"
     misc_feature    complement(4672..4688)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.15"
                     /tool="antismash"
     misc_feature    4691..4704
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.57"
                     /tool="antismash"
     gene            5001..6312
                     /locus_tag="K9S39_RS12505"
                     /old_locus_tag="K9S39_12595"
                     /pseudo=""
     CDS             5001..6312
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547187.1"
                     /locus_tag="K9S39_RS12505"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_12595"
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 8.4e-94, bitscore: 312.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERLGTGIGWRPEIAAEIAGLPGVDWVEVVAENICPGHLPPALRE
                     LRERGTTVVPHGVSLGLGGADRPAPGRLTALAERAVALGAPLVTEHIAFVRAGGPLTAS
                     PVVEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALINWPGEELTEGRFLR
                     ELVERTGVRLLIDVANLHTNHVNRGEDPAVALDELPVEAIAYVHVAGGVERDGVWHDSH
                     AHPVTQPVLDVLAELCARITPPGVLLERDEDFPEGGELAGELDAIRAVLKEARRAEAA"
     gene            6717..6902
                     /locus_tag="K9S39_RS12510"
                     /old_locus_tag="K9S39_12600"
     CDS             6717..6902
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K9S39_RS12510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="K9S39_12600"
                     /product="hypothetical protein"
                     /protein_id="WP_248863415.1"
                     /transl_table=11
                     /translation="MAHFEVNPSATWATAAGERLLRRVAAGHAGNPRPALTALAVHGPG
                     TLTEPALRTALHSSGA"
     gene            7033..8739
                     /locus_tag="K9S39_RS12515"
                     /old_locus_tag="K9S39_12605"
     CDS             7033..8739
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (92-338). E-value:
                     2.4e-19. Score: 61.8. Matches aSDomain:
                     nrpspksdomains_K9S39_RS12515_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984744.1"
                     /locus_tag="K9S39_RS12515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_12605"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_248863416.1"
                     /sec_met_domain="PF00561 (E-value: 4.3e-24, bitscore: 84.5,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRALALYGTIGSLVLTALATAPAGSADGPPLKPPAPVPHPIDFGT
                     CSPVEHLPDSVECGKVTVPLDYAHPDGKKISLTVSRIRATAPKERQGALVFNPGGPGAS
                     SMDFPLYGALARWRKVARVYDFVGYAPRGVGRSAPLSCQDPEKFAKAPTDSQRHPTEEF
                     KRKKVAQAKAYARGCVRNAGADLPFYNSVNNARDLDMIRAALGEKKLTFMGASYGTYFG
                     AVYAALFPGHIRRMVLDSVVNPEPSQIWYANNLNQNIAFQRRWRDWLKWVAKHHARYDL
                     GKTPKAVQRSYDKAVRQVRRAPVNGKIGPAQLQAAYLKTGYNDAYWAMRAQALSDYLHG
                     NPKQLIAQAEPKAGAAKEEENSNAVYTAVECNDAPWPRDWRVWDRDNTRVAKVAPFETW
                     DNAWMNLPCAFWPERSEQAVAGLEDAISRATDRATGKNADSANGDDVVDGLAAATSRTV
                     EAGLDDVLNGGSRPLDIRTAPGALPPVLLLAAERDAATPYPGALNLQRRLHGASLVTEK
                     GAGTHGIALNDNECVNKYTLAYLLRGEAPGHRVYCPARAEPKPDDRAKPMK"
     aSDomain        7309..8046
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_K9S39_RS12515_Abhydrolase_1.1"
                     /evalue="2.40E-19"
                     /label="K9S39_RS12515_Abhydrolase_1.1"
                     /locus_tag="K9S39_RS12515"
                     /protein_end="338"
                     /protein_start="92"
                     /score="61.8"
                     /tool="antismash"
                     /translation="ALVFNPGGPGASSMDFPLYGALARWRKVARVYDFVGYAPRGVGRS
                     APLSCQDPEKFAKAPTDSQRHPTEEFKRKKVAQAKAYARGCVRNAGADLPFYNSVNNAR
                     DLDMIRAALGEKKLTFMGASYGTYFGAVYAALFPGHIRRMVLDSVVNPEPSQIWYANNL
                     NQNIAFQRRWRDWLKWVAKHHARYDLGKTPKAVQRSYDKAVRQVRRAPVNGKIGPAQLQ
                     AAYLKTGYNDAYWAMRAQALSDYL"
     gene            complement(8917..9651)
                     /gene="hemQ"
                     /locus_tag="K9S39_RS12520"
                     /old_locus_tag="K9S39_12610"
     CDS             complement(8917..9651)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984746.1"
                     /locus_tag="K9S39_RS12520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_12610"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_406707916.1"
                     /transl_table=11
                     /translation="MTDASTPATAPEKTPNAGKKAKDLNEVIRYTLWSVFKLRDVLPED
                     RGGYADEVEELFAQLAAKDVVVRGTYDVSGLRADADVMIWWHSESSDALQEAYNLFRRT
                     RLGRVLEPVWSNMALHRPAEFNKSHIPAFLADEEPRDYVSVYPFVRSYDWYLLPDEDRR
                     RMLADHGKMARGFPDVRANTVPAFSLGDYEWILAFEANDLYRIVDLMRHLRGSEARLHV
                     REEIPFYTGRRKPVSELVAGLA"
     gene            complement(9663..11117)
                     /gene="hemG"
                     /locus_tag="K9S39_RS12525"
                     /old_locus_tag="K9S39_12615"
     CDS             complement(9663..11117)
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088886.1"
                     /locus_tag="K9S39_RS12525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="K9S39_12615"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_248863418.1"
                     /transl_table=11
                     /translation="MSPAHTSTGRAPGTVRHVVVIGGGISGLAAAHRLLEGGVQVTVLE
                     SSDRLGGKLRAGEIAGVPVDLGAESMLARRPEAVRLARAVGLGERLQPPATTTASLWTR
                     DALRPMPKGHVMGVPGDLDPLAASGVISPAGLARIAEDETLARTEAGEDVAVGEYVAAR
                     LGREVVDRLVEPLLGGVYAGGAYRISMRAAVPQLFEAARAERSLLEGVRAIQARAAATA
                     PPEGPVFIGIDGGIGTLPGAVADAVRAGGGEILLDSAVRELRWAGDDGWQIRADERMFQ
                     ADAVIMAAPAGAAARLLRRDCPSAAVELDTVDYASMALVTMAFRRSDLGSVPSGSGFLV
                     PPVDGHRIKASTFAGQKWGWIGDADPDLFVLRTSIGRYEDDADLKRDDSELVDLSLADL
                     GAAVGLRARPVANTVTRWHGGLPQYSVGHVRRVARIRDAVAGLPGALRVCGAVYDGVGI
                     PACIGSAHRAADDVLATLAAGTAGGE"
ORIGIN
        1 ttcgcgcagc gccgcggcgt cttcgtgtct gggtttgggc agcagcaaca catcgcactc
       61 ggcgaggagt tgctcgcggg agcgcaggcc ggccacgagg gggcgcagtg cgtcgtcggc
      121 gacgccgaac cgttcaccgt acccctgttc gaggaagatc ctctcgcgta cgtcgggggc
      181 gatccggccg aggtgaccgg ggtacaacgg cagccggaac tcgttctcct tgcgggagga
      241 ggcgagtact ccgaggctca tcaggctcat gcgcttcctc atcatttcgt tcttgtcgtg
      301 ccggttgtgg ctgccgggtt tccgggtcag gagtggtcgg cctggtgcct gcgtacgcgc
      361 tcggccagag ggaggggcgt ctcgtccaga ccgttgaccg acccgctcgc catgcgtgcg
      421 cacgttccgt ggcaggcgag cagtcgcctg tcgtcgcggt gggaaaccac caccgcctcc
      481 ggggccggcg cgctccggta gtcggtgccg cagatgaggc acgccaagcc ggagagcatc
      541 tcggcggtcg gttcggtgcg gccgcggcgg cggccggaga tcgattcctg tgaatacatg
      601 cgtcctgcat cctcttgatc ggttcagggc gtgcatgagg cgccggaata cctctcggac
      661 gccccgggaa caaggaaatc cactaccgcg gggcgcggaa cgggataccg aagacaacgc
      721 gtcaggacga gccagggggc atggtgcggc tcttttccgg ccggacgcga tgtgctccgg
      781 ccttgccgtt ccccgagggc gacgccgaca cggatgacgg catgcgaggt gcgctcggtg
      841 ccgcaaccgt actcccctcc ggacctgcag acgacccgtg agggtgtcgc cggacccttc
      901 cccgaccgac cgggaacgtt ctcggtgctc cggacgttgt agcgagtggt tgtagttgaa
      961 gtttgcgacg tgtaagcgcc cgcggagttc tgacagcggg tgcttcgccg ttttcggggg
     1021 ttttcttcga ctgctggcga ccagcagccc gggatcgcgc gagtgcgcgt tctccgacct
     1081 cctcgccatc tcgaaggaga cgtcacatgg ctcaaggaaa ccgtgaggtg gttcaacccg
     1141 gacaagggct tcggcttcat cgcccccgat gagggcggcg ccggcatctt cgtccaccac
     1201 tcggcgatcg acatgggtgg tttccgctct ttggaggaga accagcgagt ggagttcacc
     1261 gccagccagg gccccaaggg tatgcaggcc gaccaggtca gcgcgctctg aagacacccc
     1321 attccgaggc aggcccgcgc cacggtcctg cctcgcgctg cttccgaacg ggtgccgctt
     1381 ccggcgcccc cttcgcgccg gaggcagcgc ccgtgcacgc cgaaaggaac actgtggcca
     1441 ctgacatcag ctaccggggc accggtccgg cggaccacgc cgagatgacc aaacccccgc
     1501 ccgccggccg gggcagcgac tacgccgcgc tgtcccgcga ggtgaagcag agcgggctgt
     1561 tgaagcggcg gccgggctac tactccgtca aggtggggac gaacctgctc ctgctggccg
     1621 ccggctggac cgcgttcgcc ctgatcgggc agtcatggtg gcagctgctg acggccgcct
     1681 tcctcgcggt gatgttcacc cagaccgggt tcatcgggca cgacgccgga caccaccaga
     1741 tcgccgcctc caagcgcgtc aacaacctga tcggccacgt gcacgccgac ctgctgatcg
     1801 gcctcagcta cagctggtgg atctccaagc acaaccgtca ccactcccac cccaaccacg
     1861 tcgaccgcga ccccgacatc gccgacggcg ctatcgcctt cacccaggac cacgctcgcg
     1921 tccgccgcgg accgtatgcc tggctggccc gccaccaggc ctggctgttc ttcccgatgc
     1981 tgctcctgga aggactcgcc ctgcacgtcg ccggcgtacg ggcgctgctc gaccgcaccg
     2041 gcaccgcaac ctcgcggagg accaagttgg ccgaggcggg actgctgacg gcgcatctcg
     2101 gcggctatct cgccgccctg ttcctggtcc tgtcccccgt ccaggccgtg tgcttcctcc
     2161 ttgtgcacca ggggctgttc gggctctaca tggggtgctc gttcgccccc aaccacaagg
     2221 gcatgcccat cttcgccaag gacgacaaga tcgacttcct gcgtcggcag gtactgacgt
     2281 cacgcaacat ccgtggccat aggttcaccg acttcgcgct cggcgggctg aactaccaga
     2341 tcgaacacca cctgttcccc tctatgccgc ggcccagcct gcgccatgcc caggaactcg
     2401 tccgcacgtt ctgcgtccgg cgcggcatcg cctaccacga gaccggactc ttcaactcct
     2461 acgcccaggt cctgcggcac ctccacgcgg tcggcggccc cctgcgcccc gaactggaat
     2521 actgatcgcc agtccgctgc cgtaggccac gatgtcttga cgagatcacc cccgggaaaa
     2581 ctcttggggg tcggctccgt ggaactgagc gatctgttcg acgctgatgt cgtcgccgcg
     2641 gaagaaggcc aggttggtgt aggggtcctg cccctgccat gcgccagcga tccaggcaag
     2701 acgttccagc ggatcgtgcc cgcacggttg tgccgtctgc gtaccgtcca taccgccgct
     2761 gcggaatcgt ctgccccatg cggggtcgat ctggtccagc atctcggcct gcccggtgac
     2821 gaggttggtg accgggccgt acccagggga gcgggctgcg atgtgctcag cgagattgtg
     2881 gacactgtgg tggccgcgca cggtgccgtc ctcgtccagg aaggctccca gacgggaggg
     2941 atcgccccgg gaaaactctt acggtcgatg tcgtgcggaa ctcaccggat gccctcctgg
     3001 acaaccccgc gccgtctcgc cgccgccgtg gtcctctgcg cggtgttcgc gtccggctgg
     3061 ttcctcggcc agcccgtgta cgtccccgaa tgcaccatag gcccccaccg ctacagcgat
     3121 cacctggagg cggaaaactg cgacacccga ccgcgtctgc tcgcgtggtt gaacggcgac
     3181 tggagctgac cgcgaaccgg cccgctcaat gcccctcgtt gagcgggccg gtaccgttcg
     3241 tgtgccagag ttcgccggtg agggcgagga cggtgagggt gaagaggaca accgttgcga
     3301 ggaggatgag cgcggccagc gcatacacct tcccctcccc atctttccgc tgcggccgct
     3361 gtgccgcctc gatgctggcc tgaggagcag cccgcagcga cggtagccag cacccagccg
     3421 tcgttctcca ggtcccagta ctccaggaga ctgccgcacc gtatgcagtt gaccgtgtat
     3481 cgctgctgca ccatcacccc gtcagcaggg cgaaggcggc gaggaccagg acgaggccgg
     3541 cggcgacgat ggcgacggac gcccaggaac ggcggtatcc ggagtcggtg aaccgcgcag
     3601 gccgcacacc tgggtccggg tccgcgcgga acggctgcgg tccgggcggg ttgtccttcc
     3661 accgtgcggc gagcatacgg gcacgcgccg atggctcctt cggcccttgc tccgcccact
     3721 tgatatcgaa ctcgtcgggt atgtcgccct gtcgctctgt cataacgagc actgtagcga
     3781 cgccgccgga gcacgagtgg tgagatgggg cggtacagac caatccttcg ggaaggagca
     3841 ccccgccatg cgcatgcgta tgcccctcat cctcctcggc tccctcgtcg tcctggccgt
     3901 cggcgccgat ctcgccgccg agcagatagc cgagaacata gccgccaaca agttcggtga
     3961 gagactgccg gggcaacagg gcagcgccga ggtggacatc aagggcttcc ccttcctcac
     4021 ccaggccgcg accgggacgt tcgacgagat cagcgtcacc gtgaaggaca cggctgcccc
     4081 ggccggctcc tccacggtga ccctgcccag agccacgctc accgcgcgcg gagtgaaagt
     4141 ggagggcacc tcatccgccg tggccgatct ggtcgagggg caggcaaccg tcccctacag
     4201 cgcactcgca accgccctcg aacagcaaaa cccctcgggc ggaccggtgg agatagctcc
     4261 tgtcaccggc ggtgccgccc gcgtacggat cacccagggc ggacgggacc tcatcgcccg
     4321 actctcgggc cagggcgagg acatcgcgtt caccccggag gtcgagggtg cgccgccgca
     4381 ggccgtcgcc gtggctaccg ggcccctgcg ggcggacatc accggcgcca ctccggatga
     4441 gggcggagtt cggctccact tcgccgggcg gctggtcagc ctcggttagg cacctcgcgg
     4501 gactcacggc gcgcgtgagg accgccttcg aaacttgcac ccgggccccc ggacttgcca
     4561 acccgggggc cgcgcgctgc cagaagaccg cccttgaccg ggcacctctt atccggacag
     4621 gggatacctg caggtgagag cagaatgcaa ggtcccgccc ccgaacgaga tcgcaccggg
     4681 ttcgtgcacg ttgtcgccga tcatcccgca ttgcggggcc gccggcgccg acacgggcga
     4741 gagcgttccc gcctccgggc acagcgggca gggccacgag ctgtcggcgg gcgcggtcgc
     4801 aggggtcacg agctctcacc gggcacggcg ggcagggcca cgagccgcca ccgggcacgg
     4861 cggccggggt cacgagccgc cacgccccgc ccccaccgca ccaccggaag ctcaaatcaa
     4921 cctctgaacc ccgcgcgcga agggttcggc tcgccgtaac gggccatgag taccgacacc
     4981 acggagcgag agggggaagc atggagcgcc ttggcacggg aatcggctgg cggccggaga
     5041 tcgccgccga gatcgcgggg ctgcccggtg tggactgggt ggaggtcgtg gccgagaaca
     5101 tctgccccgg ccatctgccg cccgccctgc gggagctgcg cgagcgcggc acgaccgtcg
     5161 tcccgcacgg tgtctcgctc ggcctcggcg gcgcggaccg tccggccccc gggcggctga
     5221 ccgccctcgc cgaacgcgcc gtggcactgg gcgcgccgct ggtcaccgaa cacatcgcgt
     5281 tcgtccgcgc cggcgggccg ctcaccgcct caccggtggt ggaggccggg catctgctgc
     5341 ccgtgccgcg cacccgcgac gccctggacg tgctgtgcga gaacgtacgg atcgcccagg
     5401 acgcgctgcc cgtaccgctg gccctggaga acatcgcggc gctgatcaac tggccgggcg
     5461 aggagctgac cgaggggcgg ttcctccgcg aactcgtcga gcggaccggc gtacggctgc
     5521 tcatcgatgt cgccaacctc catacgaacc acgtcaaccg gggcgaggac ccggccgtcg
     5581 cactcgacga gctgccggtc gaggccatcg cgtacgtcca tgtcgcgggc ggggtggagc
     5641 gggacggggt gtggcacgac agccatgcgc acccggtgac ccagccggtg ctggacgtgc
     5701 tggccgagct ctgcgcccgg atcacgcccc ccggtgtgct gctggaacgg gatgaggact
     5761 tccccgaggg cggcgagctg gccggggagc ttgatgcgat ccgcgccgtg ctgaaggagg
     5821 cccgtcgtgc cgaggcggct tgagccggtg acggaccggt cccagggcgg gacggcagcg
     5881 ccgcccgggt ccaccgaggc ggcgcggcag cggctggcgc tcgcccagac cgcgctgctg
     5941 tccgcgctcg tcgccgggac gcccgccccg gagggtttcg accgcgcccg cttgcgggtg
     6001 cagagccggg cgctgaccgc caagcgggcc tccgtcgtcg ccaaggtggc ccccgagctg
     6061 ccggagatcc tcagcgagga cttccggatg gcgttcctcg gctacgcccg gcaccggccc
     6121 atgagcggcg gctaccggca ggacgccctg gacttcgcgg catatctgct ggcgcagggc
     6181 cgccccgacg accctgcggc acggcggcgg ttggcccagt ggtggcatga tcgcgccgga
     6241 cccgaaccgc cgtccacggg ccccgcgtcc cgtctcaccc acgccatccg gcgcgcgctg
     6301 caccgaaggt gagcgccccg cccggctgac gccgatagcc ccctgccaca cagcccacaa
     6361 acggccgcgt aacacgcaag acggaccttc gtggctcaat ccctcccggc tcccggacgc
     6421 cgccctcacc gccatagggc ccgacggcca gcgccccatc cccgcgatac gcgacggcct
     6481 ggccaccgcc gatgaggtac gggagctgtc cgaccggttc gtcggctccg gtctcgcgct
     6541 gcccgccgcg gcccctacgg ccatcgtccg ggcggtccgc caggtgaagg gcgccgttct
     6601 gctggtgctg ccgaccgccg cggcatccgt gtggactgcc cccctccggc acggagaccg
     6661 ggctgctcct ggcgtggttc gcgctgccgc tgatccttgc cgccggcacc cgggcgatgg
     6721 cccacttcga ggtcaacccg tccgccacat gggcgaccgc ggcgggcgaa cgcctgctgc
     6781 ggcgcgtcgc cgccggacac gccggaaacc cccgccccgc gctcaccgcg ctcgccgtcc
     6841 acggccccgg aaccctcacc gaacccgcac tgcggaccgc cctccactct tccggggcat
     6901 agcgcccgac accggtgcca ggtgctttac ttcggcaacc accccctcaa ctatcccttt
     6961 tgtatgcaca ccgtgcggcc cagcggagtg agcctctcgc cgtgcgccac gcaccatacg
     7021 aagggacggc tcgtgagagc actcgcgttg tacggcacca tcggatcgct ggtcctgacc
     7081 gcgctcgcca ccgccccggc ggggagcgcc gacggccctc ccctcaagcc ccccgccccg
     7141 gtgccgcacc ccatcgactt cggcacatgc tctcccgtcg agcatctgcc ggactccgtc
     7201 gaatgcggca aggtcaccgt gccgctcgac tacgcgcacc ccgacggcaa gaagatcagc
     7261 ctcaccgtca gccggatccg ggccaccgcg cccaaggagc ggcagggcgc cctggtcttc
     7321 aatcccggcg gtcccggtgc gagcagcatg gatttcccgc tctacggcgc gctcgccaga
     7381 tggcgcaagg tcgctcgcgt ctatgacttc gtcggatacg cgccgcgcgg tgtcggccgg
     7441 tccgcgccgc tctcctgcca ggacccggag aagttcgcca aggctccgac ggacagccag
     7501 cggcacccca ccgaggagtt caagcggaag aaggtggcac aggcgaaggc gtacgcgcgg
     7561 gggtgcgtcc gcaacgcggg cgccgacctg cccttttaca actcggtcaa caacgcccgc
     7621 gacctggaca tgatccgggc cgccctgggc gagaagaagc tgacgttcat gggcgcctcg
     7681 tacgggacgt acttcggtgc cgtttacgcg gcgctcttcc ccggccacat ccgccgcatg
     7741 gtcctcgaca gcgtggtcaa ccccgagccc agccagatct ggtacgccaa caacctcaac
     7801 cagaacatcg ccttccagcg ccgctggcgc gactggctca agtgggtcgc caaacaccac
     7861 gcccgatacg acctcggcaa gacgccgaag gcggtgcaac gctcgtacga caaggccgtc
     7921 cggcaggtcc gccgagcgcc cgtcaacggc aagatcggcc ccgcccagct gcaggcggcg
     7981 tatctcaaga ccggctacaa cgacgcgtac tgggcgatgc gcgcccaggc gctctcggat
     8041 tatctgcacg gcaatccgaa gcagctgatc gcccaggcgg agcccaaggc cggcgccgcc
     8101 aaggaggagg agaacagcaa cgccgtctac acggcggtcg agtgcaatga cgcgccctgg
     8161 ccgcgcgact ggcgcgtctg ggaccgcgac aacacacggg tcgcgaaggt ggcgccgttc
     8221 gagacctggg acaacgcctg gatgaatctg ccgtgcgcct tctggccgga gcggtcggag
     8281 caggccgtgg ccgggctcga agacgcgatc agcagggcca ccgaccgcgc caccgggaag
     8341 aacgccgaca gcgccaacgg cgacgatgtg gtggacgggc tggccgccgc caccagccgt
     8401 acggtcgagg ccggtctgga cgacgtgttg aacggtggtt cccggcccct cgacatccgt
     8461 acggcgcccg gcgcgctgcc cccggtgctg ctgctggcgg cggagcggga cgcggcgacg
     8521 ccgtatccgg gggcgctgaa tctgcagcgg cgactgcacg gcgcctcgct ggtcaccgag
     8581 aagggggccg gcacacacgg catcgcgctc aacgacaacg aatgcgtcaa caagtacacg
     8641 ctggcgtatc tgctgcgcgg ggaggccccg gggcaccggg tgtactgccc ggcgcgcgcg
     8701 gaaccgaagc cggacgaccg ggcgaagccc atgaagtagc gccaggtgcc gacggaaaag
     8761 cgctgcgggc cgtcccctgg gggacggccc gcagcgcttg cgcggcgagg tcaggcgagc
     8821 cccgcgacca gctcggacac cggcttccgc cgccccgtgc tgcatccccc gggggataac
     8881 ccccggaccc ccagcccaac gccccgcgca ccccgactac gccaagcccg cgaccagctc
     8941 ggacaccggc ttccgccgcc ccgtgtagaa cgggatctcc tcgcggacgt gcagacgcgc
     9001 ctcggagccg cgcaggtgcc gcatcaggtc gacgatgcgg tagaggtcat tggcctcgaa
     9061 cgccaggatc cactcgtagt cgcccagcga gaacgccggc accgtgttgg cgcggacgtc
     9121 cgggaagccg cgggccatct tgccgtggtc ggcgagcatc cggcggcggt cctcgtcggg
     9181 gaggaggtac cagtcgtagg agcgtacgaa ggggtagacg ctcacatagt cgcggggctc
     9241 ctcgtcggcc aggaaggccg ggatgtgcga cttgttgaac tcggcggggc ggtgcagcgc
     9301 catgttcgac cacaccggct ccagcacacg gccgaggcgg gtgcggcgga agaggttgta
     9361 cgcctcctgg agggcgtccg agctctccga gtgccaccag atcatgacgt cggcgtcggc
     9421 acgcaggccg gacacgtcgt aggtgccgcg cacgacgacg tccttggcgg cgagctgggc
     9481 gaacagctcc tcgacctcgt cggcgtagcc gccgcggtcc tccgggagga cgtcacgcag
     9541 cttgaacacc gaccagaggg tgtagcggat gacctcgttg aggtccttcg ccttcttacc
     9601 ggcgttcggg gtcttctcgg gagcggtggc gggggtggaa gcgtctgtca tgtcgtccat
     9661 tctcactcgc cgcccgcggt gcccgccgcc agggtggcca ggacgtcgtc ggcggcgcgg
     9721 tgcgcggagc cgatgcaggc cgggatgccg acaccgtcgt agaccgcgcc gcacacccgc
     9781 agcgccccgg gcagcccggc gaccgcgtca cggatcctgg ccacccgccg cacatggccg
     9841 acggagtact gcggcagccc gccgtgccag cgggtgaccg tgttcgcgac gggccgggcc
     9901 cgcaggccga ccgccgcgcc gaggtcggcc agcgagagat cgaccagctc ggagtcgtcg
     9961 cgcttgaggt cggcgtcgtc ctcgtaccgg ccgatggagg tgcgcagtac gaagaggtcg
    10021 gggtcggcgt ccccgatcca gccccatttc tggccggcga acgtcgatgc cttgatccgg
    10081 tgcccgtcga cgggcggtac gaggaagccg ctgccgctgg ggacgctgcc caggtccgag
    10141 cggcggaagg ccatggtgac cagcgccatc gaggcgtagt cgacggtgtc cagctcgacg
    10201 gcggcggagg ggcagtcgcg gcggagcagg cgcgccgccg ctccggccgg ggcggccatg
    10261 atcaccgcgt ccgcctggaa catccgttcg tcggcgcgga tctgccagcc gtcgtcgccg
    10321 gcccaccgca gctcccgcac ggcggagtcg aggaggatct caccgccccc cgcgcgcacg
    10381 gcgtcggcga cggcaccggg cagcgtcccg atcccgccgt cgatccctat gaagaccgga
    10441 ccttcgggcg gtgccgtggc ggccgcgcgc gcctggatgg cccgtacgcc ttcgagcagc
    10501 gaacgctcgg cacgcgcggc ctcgaagagc tgggggacgg cggcgcgcat cgagatgcgg
    10561 taggcaccgc ccgcgtagac gccgccgagc agcggctcga cgagccggtc gacgacctcg
    10621 cggccgagcc gggccgcgac atactcgccg accgcgacat cctcaccggc ctcggtccgc
    10681 gccagcgtct cgtcctcggc gatccgcgcc agaccggccg gggagatcac tccggacgcg
    10741 gcgagcggat cgaggtcccc cgggacgccc atgacatggc ccttgggcat cgggcggagc
    10801 gcgtcacggg tccacaggga cgcggtggtg gtggcgggcg gctgcagccg ttcaccgagt
    10861 ccgacggcac gcgcgagccg caccgcctcc ggccgcctgg ccagcatcga ttcggccccg
    10921 aggtcgaccg gtacacccgc gatctcaccg gcgcgcagct tgccgccgag ccggtcggac
    10981 gactccagga cggtgacctg gacgccgcct tccagaaggc ggtgcgcggc ggccagtccc
    11041 gagatgccgc ctccgatgac gacgacatga cggacggtgc ccggcgcccg gccggtactg
    11101 gtgtgcgcag ggctcatgcc cccaactctc tcagagtcag gaatgcgccg gtttcgggga
    11161 ggaggtgagg gccgggccag gacggccgca ccccccgccg gccgcccggc aaggagtccg
    11221 tccggagtcc ggaacgtgac cgcatcgcga cccgcggacg gcaacccgtc gcccctgggc
    11281 ggcgtcgaac gggcaacacc atcaagccac tt
//
