GCF_023277365.1 - 7 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_CP096975.1 (Stenotrophomonas sp. NY11291)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	RiPP-like	1,258,693	1,269,538
Region 2	RiPP-like	1,544,759	1,555,634
Region 3	lanthipeptide-class-ii	1,769,226	1,792,123
Region 4	lassopeptide	2,564,704	2,587,259
Region 5	NRP-metallophore,NRPS	2,777,832	2,826,199	High	2,3-dihydroxybenzoylserine	NRPS:Type I
Region 6	terpene-precursor	3,417,565	3,438,443
Region 7	arylpolyene	4,762,735	4,806,337	Low	APE Ec	other:other

NZ_CP096975.1 - Region 1 - RiPP-like

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Location: 1,258,693 - 1,269,538 nt. (total: 10,846 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000043.5	0.37	PKS	curacin A	Moorea producens 19L
BGC0001484.3	0.33	other (other)	2'-chloropentostatin, 2'-amino-2'-deoxyadenosine	Actinomadura sp. ATCC 39365
BGC0002000.4	0.31	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0000527.5	0.31	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0002571.2	0.31	NRPS	pyoverdine DC3000	Pseudomonas syringae pv. tomato str. DC3000
BGC0001789.3	0.31	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000453.5	0.31	NRPS	valinomycin	Streptomyces tsusimaensis
BGC0000536.3	0.31	ribosomal	nisin Q	Lactococcus lactis
BGC0000535.4	0.31	ribosomal	nisin A	Lactococcus lactis subsp. lactis
BGC0000537.3	0.31	ribosomal	nisin U	Streptococcus uberis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000043.5	0.37	PKS	curacin A	Moorea producens 19L
BGC0001484.3	0.33	other (other)	2'-chloropentostatin, 2'-amino-2'-deoxyadenosine	Actinomadura sp. ATCC 39365
BGC0002000.4	0.31	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0000527.5	0.31	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0002571.2	0.31	NRPS	pyoverdine DC3000	Pseudomonas syringae pv. tomato str. DC3000
BGC0001789.3	0.31	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000453.5	0.31	NRPS	valinomycin	Streptomyces tsusimaensis
BGC0000536.3	0.31	ribosomal	nisin Q	Lactococcus lactis
BGC0000535.4	0.31	ribosomal	nisin A	Lactococcus lactis subsp. lactis
BGC0000537.3	0.31	ribosomal	nisin U	Streptococcus uberis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (3):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.47 out of 29.38

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.90 out of 20.01

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 18.21 out of 30.97

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description

NZ_CP096975.1 - Region 2 - RiPP-like

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Location: 1,544,759 - 1,555,634 nt. (total: 10,876 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001962.3	0.27	PKS	hierridin B, hierridin C	Cyanobium sp. LEGE 06113
BGC0000188.5	0.23	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0001650.4	0.23	NRPS	Le-pyrrolopyrazines	Lysobacter enzymogenes
BGC0000452.5	0.21	NRPS	tyrocidine	Brevibacillus brevis NBRC 100599
BGC0000651.5	0.20	terpene	KS-505a	Streptomyces argenteolus
BGC0001283.3	0.18	PKS	Bisenarsan	Streptomyces lividans 1326

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001962.3	0.27	PKS	hierridin B, hierridin C	Cyanobium sp. LEGE 06113
BGC0000188.5	0.23	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0001650.4	0.23	NRPS	Le-pyrrolopyrazines	Lysobacter enzymogenes
BGC0000452.5	0.21	NRPS	tyrocidine	Brevibacillus brevis NBRC 100599
BGC0000651.5	0.20	terpene	KS-505a	Streptomyces argenteolus
BGC0001283.3	0.18	PKS	Bisenarsan	Streptomyces lividans 1326

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.19 out of 19.26

Confidence: medium

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.64 out of 26.85

Confidence: medium

Additional weak hits (2):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.95 out of 30.25

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.06 out of 26.33

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
RutR	Regulator of the pyrimidine and purine metabolism

NZ_CP096975.1 - Region 3 - lanthipeptide-class-ii

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Location: 1,769,226 - 1,792,123 nt. (total: 22,898 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase

M1L61_RS08380: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.52	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000554.4	0.48	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0002346.3	0.47	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000503.5	0.46	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0001579.4	0.46	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0002698.2	0.43	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0001576.3	0.43	ribosomal	divamide A	Prochloron didemni
BGC0000500.5	0.41	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0002316.2	0.41	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0002704.2	0.41	ribosomal	archalan β	Halorussus salinus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.52	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000554.4	0.48	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0002346.3	0.47	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000503.5	0.46	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0001579.4	0.46	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0002698.2	0.43	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0001576.3	0.43	ribosomal	divamide A	Prochloron didemni
BGC0000500.5	0.41	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0002316.2	0.41	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0002704.2	0.41	ribosomal	archalan β	Halorussus salinus

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for M1L61_RS08380

M1L61_RS08375

Leader:

MDLNDDVVSGWLNGADDVDGCANPAGSLFIYGAAATEAALTGASDTMITGG

Core:

TNECTTTVSVINHCLCC

Core with dehydrations:

DhbNECysDhbDhbDhbVDhaVINHCysLCysCys

antiSMASH-DB 4.0 matches for M1L61_RS08375

100.0%

NZ_CP011305.1and 19 others:

NZ_CP031058.1 Lanthipeptides: DUW70_RS15590_lanthipeptideNZ_CP031167.1 Lanthipeptides: MG068_RS07815_lanthipeptideNZ_CP040436.1 Lanthipeptides: FEO91_RS08085_lanthipeptideNZ_CP046588.1 Lanthipeptides: GOC52_RS08100_lanthipeptideNZ_CP060259.1 Lanthipeptides: H7F32_RS08235_lanthipeptideNZ_CP071784.1 Lanthipeptides: J3U96_RS13765_lanthipeptideNZ_CP077679.1 Lanthipeptides: KS456_RS07940_lanthipeptideNZ_CP096975.1 Lanthipeptides: M1L61_RS08375_lanthipeptideNZ_CP104071.1 Lanthipeptides: K7565_RS07800_lanthipeptideNZ_CP104287.1 Lanthipeptides: K7567_RS07875_lanthipeptideNZ_CP104324.1 Lanthipeptides: K7569_RS08320_lanthipeptideNZ_JACLBK010000002.1 Lanthipeptides: ID844_RS05155_lanthipeptideNZ_JAEDVP010000027.1 Lanthipeptides: JAK38_RS15550_lanthipeptideNZ_JAEDVW010000007.1 Lanthipeptides: JAK53_RS03160_lanthipeptideNZ_JAEDWD010000009.1 Lanthipeptides: JAK45_RS02000_lanthipeptideNZ_JAFLWT010000006.1 Lanthipeptides: J1F00_RS06425_lanthipeptideNZ_JAQQGI010000014.1 Lanthipeptides: PQS91_RS16925_lanthipeptideNZ_KB907502.1 Lanthipeptides: G370_RS0114170_lanthipeptideNZ_RHPZ01000014.1 Lanthipeptides: EGJ03_RS16365_lanthipeptide

Lanthipeptides: YH67_RS07965_lanthipeptide

1

TNECTTTVSVINHCLCC

17

TNECTTTVSVINHCLCC

1

TNECTTTVSVINHCLCC

17

24.1%

NZ_CP089924.1:

Lanthipeptides: LVB77_RS02735_lanthipeptide

4

CTTTVSVINHC

14

CT+ SV NHC

8

CTSGSSVSNHC

18

22.9%

NZ_CP011010.1and 27 others:

NZ_CP015612.1 Lanthipeptides: A7326_RS08120_lanthipeptideNZ_CP027562.1 Lanthipeptides: C6Y55_RS08375_lanthipeptideNZ_CP028358.1 Lanthipeptides: C9J74_RS08630_lanthipeptideNZ_CP040435.1 Lanthipeptides: FEO92_RS08800_lanthipeptideNZ_CP040438.1 Lanthipeptides: FEO89_RS08350_lanthipeptideNZ_CP040439.1 Lanthipeptides: FEO88_RS08780_lanthipeptideNZ_CP043578.1 Lanthipeptides: F0Q13_RS08255_lanthipeptideNZ_CP047310.1 Lanthipeptides: GS396_RS07720_lanthipeptideNZ_CP056088.1 Lanthipeptides: HUZ96_RS08220_lanthipeptideNZ_CP067993.1 Lanthipeptides: JJL50_RS07700_lanthipeptideNZ_CP090418.1 Lanthipeptides: LYZ64_RS08310_lanthipeptideNZ_CP104169.1 Lanthipeptides: NDR96_RS06465_lanthipeptideNZ_CP109812.1 Lanthipeptides: FQS62_RS08595_lanthipeptideNZ_DS999412.1 Lanthipeptides: SSKA14_RS15505_lanthipeptideNZ_JABDRV010000001.1 Lanthipeptides: HRL60_RS01085_lanthipeptideNZ_JAEDUZ010000008.1 Lanthipeptides: JAK52_RS03215_lanthipeptideNZ_JAEDVS010000025.1 Lanthipeptides: JAK50_RS15965_lanthipeptideNZ_JAEDWK010000010.1 Lanthipeptides: JAK51_RS04155_lanthipeptideNZ_JAJNMF010000022.1 Lanthipeptides: LQ423_RS11465_lanthipeptideNZ_JVHP01000063.1 Lanthipeptides: ADI44_RS21105_lanthipeptideNZ_JVPM01000038.1 Lanthipeptides: ADH50_RS19075_lanthipeptideNZ_JVUI01000059.1 Lanthipeptides: ADG58_RS15825_lanthipeptideNZ_LR134301.1 Lanthipeptides: EL200_RS07985_lanthipeptideNZ_OBEE01000002.1 Lanthipeptides: CRO53_RS02030_lanthipeptideNZ_OU943334.1 Lanthipeptides: LNN95_RS09005_lanthipeptideNZ_RHQE01000015.1 Lanthipeptides: EGJ89_RS13150_lanthipeptideNZ_SOCO01000001.1 Lanthipeptides: N440_RS05690_lanthipeptide

Lanthipeptides: VN11_RS08230_lanthipeptide

8

VSVINHCLCC

17

+SV+NHCLCC

26

MSVVNHCLCC

35

with 1 more sequence matches

13.8%

NZ_CP007597.1:

Lanthipeptides: DX03_RS09815_lanthipeptide

8

VSVINHCLCC

17

+SVINHC+CC

49

MSVINHCMCC

58

allorf_1778660_1778776

Leader:

MSLRLPSIRSRMKAA

Core:

RPLIPSCFRAAHAALPRACRSAA

Core with dehydrations:

RPLIPDhaCysFRAAHAALPRACysRDhaAA

antiSMASH-DB 4.0 matches for allorf_1778660_1778776

100.0%

NZ_CP011305.1and 4 others:

NZ_CP046588.1 Lanthipeptides: allorf_1748975_1749091_lanthipeptideNZ_CP096975.1 Lanthipeptides: allorf_1778660_1778776_lanthipeptideNZ_JAEDVP010000027.1 Lanthipeptides: allorf_23874_23990_lanthipeptideNZ_RHPZ01000014.1 Lanthipeptides: allorf_016179_016295_lanthipeptide

Lanthipeptides: allorf_1722617_1722733_lanthipeptide

1

RPLIPSCFRAAHAALPRACRSAA

23

RPLIPSCFRAAHAALPRACRSAA

1

RPLIPSCFRAAHAALPRACRSAA

23

91.3%

NZ_CP047310.1:

Lanthipeptides: allorf_1658784_1658924_lanthipeptide

1

RPLIPSCFRAAHAALPRACRSAA

23

RP IPS FRAAHAALPRACRSAA

1

RPRIPSRFRAAHAALPRACRSAA

23

34.8%

NZ_OBEE01000002.1and 1 other:

NZ_RHQE01000015.1 Lanthipeptides: allorf_094530_094610_lanthipeptide

Lanthipeptides: allorf_016795_016875_lanthipeptide

16

PRACRSAA

23

PRACRSAA

2

PRACRSAA

9

with 5 more sequence matches

29.2%

NZ_QGTL01000006.1:

Lanthipeptides: allorf_311998_312129_lanthipeptide

3

LIPSCFRAAHAALPR

17

LIP+C + +ALP

10

LIPACAGSTPSALPT

24

26.1%

NZ_CP011130.1and 3 others:

NZ_CP023465.1 Lanthipeptides: allorf_5424882_5424968_lanthipeptideNZ_CP076103.1 Lanthipeptides: allorf_5482973_5483059_lanthipeptideNZ_LMHM01000001.1 Lanthipeptides: allorf_734482_734568_lanthipeptide

Lanthipeptides: allorf_5359517_5359603_lanthipeptide

10

AAHAALPRACRSA

22

A + +PR CR A

5

AGTSCMPRKCRGA

17

15.1%

NZ_FNIE01000012.1:

Lanthipeptides: allorf_231668_231853_lanthipeptide

4

IPSCFRAAHAALPRAC

19

+P RA + ALPR C

12

LPHRRRATYWALPRVC

27

15.0%

NZ_CP015405.2and 1 other:

NZ_CP053228.1 Lanthipeptides: HL650_RS16700_lanthipeptide

Lanthipeptides: A4V09_RS22690_lanthipeptide

2

PLIPSCFRAAHAA

14

I SCF A H

6

DYIYSCFEALHTT

18

15.0%

NZ_JALAKW010000008.1and 2 others:

NZ_JALAME010000008.1 Lanthipeptides: allorf_051109_051318_lanthipeptideNZ_JALAYD010000020.1 Lanthipeptides: allorf_33824_34033_lanthipeptide

Lanthipeptides: allorf_059632_059841_lanthipeptide

3

LIPSCFRAAHAALPR

17

L+P C + A++PR

16

LLPHCLNSFPASIPR

30

MIBiG 4.0 matches for allorf_1778660_1778776

7.8%

BGC0002120.3 (NQE92413.1):

Lanthipeptide: nocathioamide A

1

RPLIPSC

7

RP P+C

34

RPAAPAC

40

allorf_1786054_1786242

Leader:

MRDVYVIDQLFRCSRFCKTGGKHNARYCDAQGRLPGS

Core:

HSERACRGIGTPPSLTCNGRIRRGT

Core with dehydrations:

HDhaERACysRGIGDhbPPDhaLDhbCysNGRIRRGDhb

antiSMASH-DB 4.0 matches for allorf_1786054_1786242

100.0%

NZ_CP011305.1and 12 others:

NZ_CP047310.1 Lanthipeptides: allorf_1666200_1666388_lanthipeptideNZ_CP060259.1 Lanthipeptides: allorf_1761351_1761539_lanthipeptideNZ_CP077679.1 Lanthipeptides: allorf_1698369_1698557_lanthipeptideNZ_CP096975.1 Lanthipeptides: allorf_1786054_1786242_lanthipeptideNZ_CP104169.1 Lanthipeptides: allorf_1387914_1388102_lanthipeptideNZ_JABDRV010000001.1 Lanthipeptides: allorf_0209381_0209569_lanthipeptideNZ_JAEDVP010000027.1 Lanthipeptides: allorf_16408_16596_lanthipeptideNZ_JAEDVS010000025.1 Lanthipeptides: allorf_344793_344981_lanthipeptideNZ_JAQQGI010000014.1 Lanthipeptides: allorf_023910_024098_lanthipeptideNZ_JVPM01000038.1 Lanthipeptides: allorf_471579_471767_lanthipeptideNZ_OU943334.1 Lanthipeptides: allorf_1884881_1885069_lanthipeptideNZ_RHPZ01000014.1 Lanthipeptides: allorf_023573_023761_lanthipeptide

Lanthipeptides: allorf_1730011_1730199_lanthipeptide

1

HSERACRGIGTPPSLTCNGRIRRGT

25

HSERACRGIGTPPSLTCNGRIRRGT

1

HSERACRGIGTPPSLTCNGRIRRGT

25

MIBiG 4.0 matches for allorf_1786054_1786242

20.0%

BGC0000563.4 (AEA03263.1):

Lanthipeptide: venezuelin

6

CRGIGTPPSLTCNG

19

C G+ T + C G

5

CVGLLTLLNTVCIG

18

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 21.78 out of 29.72

Confidence: medium

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 27.95 out of 32.74

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

Additional weak hits (5):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.46 out of 32.74

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.79 out of 29.65

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.90 out of 20.01

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.90 out of 30.22

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.77 out of 23.43

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

M1L61_RS08375 - Class II

Cleavage pHMM score: -4.40

RODEO score: 0

Monoisotopic mass: 1749.8 Da

Molecular weight: 1751.0 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 1769.0 Da N = 1

allorf_1778660_1778776 - Class II

Cleavage pHMM score: -5.80

RODEO score: 0

Monoisotopic mass: 2398.3 Da

Molecular weight: 2399.8 Da

Number of bridges: 2

allorf_1786054_1786242 - Class II

Cleavage pHMM score: -6.30

RODEO score: 0

Monoisotopic mass: 2604.4 Da

Molecular weight: 2605.9 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2624.0 Da N = 1; 2642.0 Da N = 2; 2660.0 Da N = 3

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
AfsR	Pleiotropic regulatory for antibiotic production
FuR variant 2	Ferric Uptake Regulator
NtrC	Nitrogen regulatory protein C

NZ_CP096975.1 - Region 4 - lassopeptide

Download region GenBank file

Download region SVG

Location: 2,564,704 - 2,587,259 nt. (total: 22,556 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

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Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

M1L61_RS12075: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000576.3	0.51	ribosomal	rubrivinodin	Rubrivivax gelatinosus IL144
BGC0000577.3	0.50	ribosomal	sphingonodin II	Sphingobium japonicum UT26S
BGC0001786.3	0.48	ribosomal	subterisin	Novosphingobium subterraneum
BGC0000573.3	0.45	ribosomal	caulonodin I, caulonodin II	Caulobacter sp. K31
BGC0001779.5	0.44	ribosomal	siamycin	Streptomyces nodosus
BGC0002015.4	0.42	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0002307.3	0.42	ribosomal	humidimycin	Streptomyces humidus
BGC0001645.3	0.42	ribosomal	lagmysin	Streptomyces sp. NRRL S-118
BGC0000519.4	0.41	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000616.4	0.40	ribosomal	amylocyclicin	Bacillus velezensis FZB42

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000576.3	0.51	ribosomal	rubrivinodin	Rubrivivax gelatinosus IL144
BGC0000577.3	0.50	ribosomal	sphingonodin II	Sphingobium japonicum UT26S
BGC0001786.3	0.48	ribosomal	subterisin	Novosphingobium subterraneum
BGC0000573.3	0.45	ribosomal	caulonodin I, caulonodin II	Caulobacter sp. K31
BGC0001779.5	0.44	ribosomal	siamycin	Streptomyces nodosus
BGC0002015.4	0.42	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0002307.3	0.42	ribosomal	humidimycin	Streptomyces humidus
BGC0001645.3	0.42	ribosomal	lagmysin	Streptomyces sp. NRRL S-118
BGC0000519.4	0.41	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000616.4	0.40	ribosomal	amylocyclicin	Bacillus velezensis FZB42

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lasso peptide predictions

Lassopeptide(s) for M1L61_RS12085

M1L61_RS12085

Leader:

MNELIELGVVAGEQNHEDPSELIELGSVVSETK

Full core:

AHNHGTLWDGVFPTTSP

Putative macrolactam:

AHNHGTLWD

antiSMASH-DB 4.0 matches for M1L61_RS12085

100.0%

NC_011071.1and 41 others:

NZ_AP021867.1 Lassopeptides: GJU96_RS20605_lassopeptideNZ_CP011010.1 Lassopeptides: VN11_RS22530_lassopeptideNZ_CP011305.1 Lassopeptides: YH67_RS21020_lassopeptideNZ_CP015612.1 Lassopeptides: A7326_RS21600_lassopeptideNZ_CP027562.1 Lassopeptides: C6Y55_RS23185_lassopeptideNZ_CP029773.1 Lassopeptides: DM611_RS22725_lassopeptideNZ_CP031058.1 Lassopeptides: DUW70_RS11975_lassopeptideNZ_CP031167.1 Lassopeptides: MG068_RS21080_lassopeptideNZ_CP037858.1 Lassopeptides: EZ304_RS20935_lassopeptideNZ_CP040432.1 Lassopeptides: FEO95_RS22840_lassopeptideNZ_CP040438.1 Lassopeptides: FEO89_RS21625_lassopeptideNZ_CP040439.1 Lassopeptides: FEO88_RS23220_lassopeptideNZ_CP043578.1 Lassopeptides: F0Q13_RS11365_lassopeptideNZ_CP046588.1 Lassopeptides: GOC52_RS10870_lassopeptideNZ_CP047310.1 Lassopeptides: GS396_RS20180_lassopeptideNZ_CP060259.1 Lassopeptides: H7F32_RS11180_lassopeptideNZ_CP067993.1 Lassopeptides: JJL50_RS10815_lassopeptideNZ_CP077679.1 Lassopeptides: KS456_RS10490_lassopeptideNZ_CP080573.1 Lassopeptides: K1516_RS11235_lassopeptideNZ_CP090418.1 Lassopeptides: LYZ64_RS11980_lassopeptideNZ_CP096975.1 Lassopeptides: M1L61_RS12085_lassopeptideNZ_CP104169.1 Lassopeptides: NDR96_RS09175_lassopeptideNZ_CP106759.1 Lassopeptides: N8888_RS09665_lassopeptideNZ_CP120400.1 Lassopeptides: PVE91_RS09960_lassopeptideNZ_JABDRV010000002.1 Lassopeptides: HRL60_RS08285_lassopeptideNZ_JADCQA010000003.1 Lassopeptides: IMX13_RS03920_lassopeptideNZ_JAEDVS010000016.1 Lassopeptides: JAK50_RS09365_lassopeptideNZ_JAEDVW010000040.1 Lassopeptides: JAK53_RS10000_lassopeptideNZ_JAEDWA010000005.1 Lassopeptides: JAK63_RS01795_lassopeptideNZ_JAEDWK010000019.1 Lassopeptides: JAK51_RS11395_lassopeptideNZ_JAEDWU010000002.1 Lassopeptides: JAK37_RS06750_lassopeptideNZ_JAKJQX010000001.1 Lassopeptides: MT466_RS00850_lassopeptideNZ_JAQQGI010000001.1 Lassopeptides: PQS91_RS01155_lassopeptideNZ_JVHP01000037.1 Lassopeptides: ADI44_RS25485_lassopeptideNZ_JVPM01000019.1 Lassopeptides: ADH50_RS20965_lassopeptideNZ_JVUI01000038.1 Lassopeptides: ADG58_RS23065_lassopeptideNZ_OBEE01000001.1 Lassopeptides: CRO53_RS21815_lassopeptideNZ_OU943334.1 Lassopeptides: LNN95_RS11880_lassopeptideNZ_RHPZ01000001.1 Lassopeptides: EGJ03_RS21120_lassopeptideNZ_RHQE01000001.1 Lassopeptides: EGJ89_RS22430_lassopeptideNZ_SOCO01000001.1 Lassopeptides: N440_RS20340_lassopeptide

Lassopeptides: SMAL_RS21110_lassopeptide

1

AHNHGTLWDGVFPTTSP

17

AHNHGTLWDGVFPTTSP

1

AHNHGTLWDGVFPTTSP

17

88.2%

NZ_CP058243.1:

Lassopeptides: AD14011_RS10295_lassopeptide

1

AHNHGTLWDGVFPTTSP

17

AH HGTLWDG+FPTTSP

1

AHGHGTLWDGIFPTTSP

17

61.1%

NZ_CP049017.1:

Lassopeptides: G4Q83_RS07645_lassopeptide

1

AHNHGTLWDGVF-PTTSP

17

AHNHGT + F TTSP

1

AHNHGTFLEASFIATTSP

18

with 5 more sequence matches

29.2%

NZ_CP033362.1:

Lassopeptides: EB232_RS01185_lassopeptide

6

TLWDGVFPTTSP

17

T DGVFP +P

6

TALDGVFPIHTP

17

22.2%

NZ_CP044492.1and 1 other:

NZ_CP066294.2 Lanthipeptides: IGS65_RS08185_lanthipeptide

Lanthipeptides: FSA31_RS00605_lanthipeptide

8

WDGVFPTTS

16

W GV PT S

4

WQGVVPTVS

12

22.2%

NZ_CP049223.1and 4 others:

NZ_CP049225.1 Lanthipeptides: G6Z85_RS06555_lanthipeptideNZ_CP049228.1 Lanthipeptides: G6Z83_RS06555_lanthipeptideNZ_JAKWZB010000003.1 Lanthipeptides: MMO78_RS01405_lanthipeptideNZ_PNGO01000002.1 Lanthipeptides: CJ224_RS02730_lanthipeptide

Lanthipeptides: G6Z86_RS06445_lanthipeptide

8

WDGVFPTTS

16

W GV PT S

4

WQGVLPTVS

12

22.2%

NZ_FNUH01000004.1and 1 other:

NZ_FOGM01000011.1 Lanthipeptides: BMX20_RS07220_lanthipeptide

Lanthipeptides: BLU50_RS05815_lanthipeptide

7

LWDGVFPTTS

16

W GV PT S

3

FWQGVVPTVS

12

13.9%

NZ_CP015883.1and 1 other:

NZ_JAASJP010000011.1 Lanthipeptides: allorf_43013_43183_lanthipeptide

Lanthipeptides: allorf_0205278_0205448_lanthipeptide

3

NHGTLWDGVF

12

N+G +W+ VF

16

NNGCMWNTVF

25

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 20.85 out of 23.79

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.26 out of 19.26

Confidence: medium

Additional weak hits (9):

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 25.78 out of 33.14

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.29 out of 24.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.39 out of 26.06

Confidence: weak

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 22.27 out of 32.93

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 26.30 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 20.01 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.98 out of 28.19

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.64 out of 29.65

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.67 out of 29.65

Confidence: weak

Lasso peptides

RREFinder

TFBS Finder

Core peptide predictions

M1L61_RS12085 - Class II: Cleavage pHMM score: 10.60; RODEO score: 23; Monoisotopic mass: 1817.8 Da; Molecular weight: 1818.9 Da; Number of bridges: 0

RRE predictions

: RRE type: Lasso_Fused_RRE; Location: 5 - 76; Score: 54.9

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
IolR	Regulator of the inositol catabolism

NZ_CP096975.1 - Region 5 - NRP-metallophore,NRPS

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Location: 2,777,832 - 2,826,199 nt. (total: 48,368 nt) Show pHMM detection rules used

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

M1L61_RS13060: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

M1L61_RS13100: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

M1L61_RS13120: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

Hydrolases: hydrolase

M1L61_RS13075: isochorismatase

smcogs: SMCOG1027: isochorismatase

Isomerases: isomerase

M1L61_RS13085: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase

M1L61_RS13065: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

M1L61_RS13080: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002689.2	1.33	NRPS	2,3-dihydroxybenzoylserine	Stenotrophomonas maltophilia K279a
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000343.5	0.63	NRPS	enterobactin	Pseudomonas sp. J465
BGC0001135.5	0.60	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002689.2	0.87	NRPS	2,3-dihydroxybenzoylserine	Stenotrophomonas maltophilia K279a
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.68	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.67	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.66	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000343.5	0.65	NRPS	enterobactin	Pseudomonas sp. J465
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.12 out of 23.79

Confidence: medium

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.54 out of 26.33

Confidence: medium

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 18.67 out of 20.01

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.19 out of 19.26

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

Additional weak hits (25):

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.97 out of 30.98

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.36 out of 20.01

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.81 out of 26.06

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.98 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.08 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.16 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.59 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 26.18 out of 32.74

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 23.91 out of 30.97

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 21.81 out of 31.73

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 27.09 out of 39.62

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.91 out of 29.38

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.92 out of 26.85

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.54 out of 30.25

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 18.70 out of 26.12

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 27.45 out of 39.62

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.56 out of 28.14

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 23.92 out of 39.62

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 27.05 out of 39.62

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.64 out of 21.84

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.08 out of 26.85

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 26.35 out of 33.14

Confidence: weak

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.94 out of 19.52

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.44 out of 33.14

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 5, location 2777831 - 2826199:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

M1L61_RS13065: Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (458..845): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWELHFFTSGEHNLYGPTEAAVDVSY
Nearest Stachelhaus code(s):: Ser DVWHFSLVDK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

M1L61_RS13080: diOH-Bz

Search NORINE for peptide: strict or relaxed

AMP-binding (39..444): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: SASLFPMSSPGGGLQVGGAQQVFGMAEGLVNYTR
Nearest Stachelhaus code(s):: diOH-Bz PMPAQGVVNK (94% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
AfsR	Pleiotropic regulatory for antibiotic production
ArgR	Regulator of arginine biosynthesis genes
CRP	Cyclic AMP receptor protein
IolR	Regulator of the inositol catabolism

NZ_CP096975.1 - Region 6 - terpene-precursor

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Location: 3,417,565 - 3,438,443 nt. (total: 20,879 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: synthetase

M1L61_RS16065: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000643.3	0.33	terpene	carotenoid	Brevundimonas vesicularis
BGC0000634.3	0.32	terpene	carotenoid	Brevundimonas sp. SD212
BGC0000640.3	0.32	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000630.3	0.31	terpene	(2R,3S,3'S)-2-hydroxyastaxanthin	Paracoccus haeundaensis
BGC0002219.2	0.31	terpene	(-)-(R)-nephthenol	Stereum hirsutum FP-91666 SS1
BGC0000641.3	0.30	terpene	carotenoid	Enterobacteriaceae bacterium DC416
BGC0000639.3	0.30	terpene	carotenoid	Enterobacteriaceae bacterium DC260

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000643.3	0.33	terpene	carotenoid	Brevundimonas vesicularis
BGC0000634.3	0.32	terpene	carotenoid	Brevundimonas sp. SD212
BGC0000640.3	0.32	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000630.3	0.31	terpene	(2R,3S,3'S)-2-hydroxyastaxanthin	Paracoccus haeundaensis
BGC0002219.2	0.31	terpene	(-)-(R)-nephthenol	Stereum hirsutum FP-91666 SS1
BGC0000641.3	0.30	terpene	carotenoid	Enterobacteriaceae bacterium DC416
BGC0000639.3	0.30	terpene	carotenoid	Enterobacteriaceae bacterium DC260

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

M1L61_RS16065: PT_FPPS_like

Domain 1 (32 - 292): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: DMAPP, IPP → FPP

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.20 out of 23.79

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.13 out of 19.26

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 19.52 out of 19.52

Confidence: strong

Additional weak hits (5):

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.63 out of 18.57

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.42 out of 18.57

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.54 out of 26.06

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.64 out of 21.84

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.56 out of 29.25

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
CRP	Cyclic AMP receptor protein
IolR	Regulator of the inositol catabolism

NZ_CP096975.1 - Region 7 - arylpolyene

Download region GenBank file

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Location: 4,762,735 - 4,806,337 nt. (total: 43,603 nt) Show pHMM detection rules used

arylpolyene: (APE_KS1 or APE_KS2)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

M1L61_RS22275: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

M1L61_RS22340: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acyltransferase halogenase

M1L61_RS22360: conventional flavin-dependent halogenase

halogenases: conventional flavin-dependent halogenase
smcogs: SMCOG1119: halogenase

M1L61_RS22415: acyltransferase

smcogs: SMCOG1141: acyltransferase

Hydrolases: hydrolase

M1L61_RS22295: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Ligases: ligase synthase

M1L61_RS22335: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

M1L61_RS22350: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

M1L61_RS22390: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

M1L61_RS22395: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002341.2	0.75	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002608.2	0.75	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0002008.3	0.63	other (other)	aryl polyenes	Xenorhabdus doucetiae
BGC0000836.5	0.62	other (other)	APE Ec	Escherichia coli CFT073
BGC0002276.2	0.60	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001895.4	0.50	PKS	huanglongmycin A, huanglongmycin B, huanglongmycin C	Streptomyces sp. CB09001
BGC0002000.4	0.49	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0001723.5	0.49	PKS	oviedomycin	Streptomyces antibioticus
BGC0002047.2	0.49	PKS	brasiliquinone A, brasiliquinone B	Nocardia brasiliensis
BGC0000837.4	0.49	other (other)	APE Vf	Aliivibrio fischeri ES114

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002341.2	0.75	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002608.2	0.75	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0002008.3	0.63	other (other)	aryl polyenes	Xenorhabdus doucetiae
BGC0000836.5	0.62	other (other)	APE Ec	Escherichia coli CFT073
BGC0002276.2	0.60	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001895.4	0.50	PKS	huanglongmycin A, huanglongmycin B, huanglongmycin C	Streptomyces sp. CB09001
BGC0002000.4	0.49	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0001723.5	0.49	PKS	oviedomycin	Streptomyces antibioticus
BGC0002047.2	0.49	PKS	brasiliquinone A, brasiliquinone B	Nocardia brasiliensis
BGC0000837.4	0.49	other (other)	APE Vf	Aliivibrio fischeri ES114

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.09 out of 20.47

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.99 out of 20.47

Confidence: medium

Additional weak hits (12):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.07 out of 21.84

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.42 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.06 out of 26.06

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.06 out of 26.33

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.00 out of 23.43

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.31 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.06 out of 26.06

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.76 out of 27.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.07 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.01 out of 26.06

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.21 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.57 out of 28.19

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

TFBS Finder

NRPS/PKS substrate predictions

M1L61_RS22395: X

AMP-binding (15..302): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: THQ-----LFRWVAFSSGGTEVYGSSETGGIAWR
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems