LOCUS       NZ_CP096281            11368 bp    DNA     linear   CON 08-JUN-2024
DEFINITION  Streptomyces sp. WAC00303 chromosome.
ACCESSION   NZ_CP096281
VERSION     NZ_CP096281.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. WAC00303
  ORGANISM  Streptomyces sp. WAC00303
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8548838)
  AUTHORS   Meyer,K.J., Tan,S. and Nodwell,J.R.
  TITLE     Genome Sequence of WAC00303 Direct Submission
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8548838)
  AUTHORS   Meyer,K.J., Tan,S. and Nodwell,J.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Biochemistry, University of Toronto, 1 Kings
            College Circle 5207, Toronto, ON M5S 1A8, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-MAR-2022
            Assembly Method                   :: Flye v. 2.8.1
            Assembly Name                     :: WAC00303
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58.0x
            Sequencing Technology :: Oxford Nanopore; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023210535.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 05:21:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,823
            CDSs (total)                      :: 7,731
            Genes (coding)                    :: 7,293
            CDSs (with protein)               :: 7,293
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 438
            CDSs (without protein)            :: 438
            Pseudo Genes (ambiguous residues) :: 0 of 438
            Pseudo Genes (frameshifted)       :: 228 of 438
            Pseudo Genes (incomplete)         :: 241 of 438
            Pseudo Genes (internal stop)      :: 26 of 438
            Pseudo Genes (multiple problems)  :: 54 of 438
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:23:54
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7854077
            Orig. end                         :: 7865445
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096281.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Justin Nodwell, University
            of Toronto.
FEATURES             Location/Qualifiers
     region          1..11368
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="30"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11368
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11368
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6368](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6368
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            45..1100
                     /locus_tag="MWG59_RS35915"
                     /old_locus_tag="MWG59_35915"
     CDS             45..1100
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1011: transport
                     system permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955794.1"
                     /locus_tag="MWG59_RS35915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_35915"
                     /product="iron ABC transporter permease"
                     /protein_id="WP_097937465.1"
                     /transl_table=11
                     /translation="MTLIETMPGPARRALCAPGVRMACLALLVAAVACADLFAGRGVTP
                     EGVREVLLGGGDPTAEHIVLQLRLPRLLVALVAGACLGVAGLVLQSALRNPLAGPEVTG
                     VTPGAVLGAVTATGLGLAGWESPTAVVVAACLGGFAGAGLLWLLAGREAGDPEQTAVYG
                     VLVSAVLAGLTAVVLLVAPGELGSVVQWLIGSTEGRVWQHWNLLWPWAVFWGAAAWLLA
                     GPLTLLRCGDEQASAAGLATGRGRGAALVCAVALTAGAVSAVGALGFVGLLVPHLALAV
                     FGADLRITLPGAALLGATVVCGADAAAQLLSRLLATALEADRLTLPVGALTTFVGAGLL
                     MVVARRRADER"
     gene            1267..2097
                     /locus_tag="MWG59_RS35920"
                     /old_locus_tag="MWG59_35920"
     CDS             1267..2097
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006129276.1"
                     /locus_tag="MWG59_RS35920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_35920"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_098014281.1"
                     /transl_table=11
                     /translation="MPGPEVVRAEGLAFGYPGRMVLRGVDLSVRAGELVALIGLNGCGK
                     STFLKLAAGLLAPSGGRVLLEGDDVTRLPRRTAARRVALLHQSAPTVPGLTVRQLVRQG
                     RYAARGPLGMLREGDDEVTSRALADVGVTTWADRPVDALSGGERQRVRLAMALAQDARV
                     LFLDEPTTYLDLRHQLEVLQTVVRLRSERRLTVVMVLHDLNHAARFADRIVALREGRVA
                     ADGPPAEVVTRRLLAEVLGVEGRVGIDSEGGWPVCYPDHPLDPLQLLRNENHIH"
     misc_feature    complement(2165..2182)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 13.87"
                     /tool="antismash"
     gene            2172..2306
                     /gene="amiA"
                     /locus_tag="MWG59_RS35925"
                     /old_locus_tag="MWG59_35925"
     CDS             2172..2306
                     /codon_start=1
                     /gene="amiA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508296.1"
                     /locus_tag="MWG59_RS35925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_35925"
                     /product="streptamidine family RiPP"
                     /protein_id="WP_248586791.1"
                     /transl_table=11
                     /translation="MENNKVFSPIADQGQLAHLSATHSNALVENPFDDAVEADIAAEK"
     gene            2390..3364
                     /locus_tag="MWG59_RS35930"
                     /old_locus_tag="MWG59_35930"
     CDS             2390..3364
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452727.1"
                     /locus_tag="MWG59_RS35930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_35930"
                     /product="hypothetical protein"
                     /protein_id="WP_248586792.1"
                     /transl_table=11
                     /translation="MTRSRLAPGTGIVTVPGGAPVLRTSGGEFLRIDTGGVTGEDLVRR
                     LTGEGEPEEETPDEGAHTAELTRLVQAFEAAGYALSTPPRAPLAGRTVHVLGDPALTAP
                     VVRCAGAEGAEVHRITPDGVAELARATPDEGAEGRVAVVWCLDSPVPDGLWDTADRLPG
                     RRIAWLRCHREGAHSWIEPLAAAPGDITSRHIRLRRLAATPAHRELAAYWAGHRTPDTG
                     PRPTEASAALIAALLTADLIAWAEAEAEAEAGEGEGEGEGEGVGAGAGTGRAAVLPARR
                     RLRRIDLRDLTLTEHPVLPVPDVAPLPAPAAVRTRAGTAEARL"
     gene            3361..5004
                     /locus_tag="MWG59_RS35935"
                     /old_locus_tag="MWG59_35935"
     CDS             3361..5004
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377868.1"
                     /locus_tag="MWG59_RS35935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_35935"
                     /product="CocE/NonD family hydrolase"
                     /protein_id="WP_248586793.1"
                     /transl_table=11
                     /translation="MSTRTVYVPTPDGTALATDVCLPDGTRPLPAVLIRTPYGREAHRT
                     ELRAWAAHGFAALAQDVCGRHGSPGEWHPYRDHERTNGAATVAWVRAQAWSNGEVVAVG
                     ASYAAYCALVTALATPEAACDTGPDADPGDFYRDGVPDAVIAAVPALGLTETAREPGGP
                     ERLWARAGWWAAHGDRRDSDPDALARALADDPRLLEHLPVARLAERLAASLGRELPSWS
                     GLWADRARGRLVALGSTARLPLLAVGGTRDPFAEDTVALWRGWGGPSRLLLGPWGHRLT
                     ADPAAPARARVNLGALYVRWARAALSGRLEPTRHGVIALGDSGRWHTTRLRSTPRPASA
                     QDATPQDATEREGADRPEAESRWSFDSPTGLRLLHGAEFNADPDRPVRSDDLAVPADGD
                     RADRCLLLSPPLPRPLDLAGAAVARINVTADTPCADWAVRLTALDPTGRADPLAFGIVR
                     RTGPPGEAADISVPLGTLGRRLPAGTRLRAEIAGHHFPAHARNPHTGEDPVTATRLAPS
                     RRTVDPRGSALHLPVVARRRYVEPAPEICR"
     gene            5001..6368
                     /locus_tag="MWG59_RS35940"
                     /old_locus_tag="MWG59_35940"
     CDS             5001..6368
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059075.1"
                     /locus_tag="MWG59_RS35940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_35940"
                     /product="YcaO-like family protein"
                     /protein_id="WP_248587350.1"
                     /sec_met_domain="YcaO (E-value: 1.3e-43, bitscore: 148.8,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSALPIEALVDPVSGIIRDVAPVEHPTGAPPRYTAMTADVADAR
                     RLGAWPADRVSLGTTFGDPRGAWIAAVAEAVERYCGNRLPPPGHPQEPRRATAAELAAE
                     GHRLYGPGALPAYAPWQYVRPGFPYSELTSETETLWTRVTENGRPCWAPVALTHLNWRQ
                     GELRSLPRTHHLNYAGIATGQGLDDAVERGLLEVVERDALELWWHLDGPTRGIDPASVP
                     GLTEDLAGCGLDVHIVEMPSEFAPCVAALAHDPVRGIYAAGFACRYDPAEAARKAVLEA
                     VHTWVFTQGAVDADGWVYRAVEAGMLARGLYLDHRADRRYLDDCGPEFAAVRDLGAHVQ
                     VWLDDRMAPLARRFTEPALGTVPVTAIDPGSPTALDTALAAGGHRVITADLTTEDVAET
                     SLRVARVLVSGLIPNAPAAFGYFGCPRFADAALARGWRTRPPAAPGDFTLAPPPHM"
     gene            6383..>7774
                     /locus_tag="MWG59_RS35945"
                     /old_locus_tag="MWG59_35945"
                     /pseudo=""
     CDS             6383..>7774
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452724.1"
                     /locus_tag="MWG59_RS35945"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /old_locus_tag="MWG59_35945"
                     /product="nitroreductase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MPDTSARTVAPESGTRPTSGLLRRALADVRSSRAPGLAPYVPERP
                     FPRSGAGLPLAGDEARPGAGPGAGGRAAIDLDRVLRLSLAAPADDTGPAAPRPLGGSPA
                     PGARPPPDRSGVFAAARTLRLRPARPPRPPARPGARRRVPGCARRPHRGRVAHRRALRP
                     PRLAAAAAGHGPRRRRPGTGSAPAADVLVCLDADAALLSAAAGLPAAPDRPDTWPEAEP
                     ELPLAAVRLTPTGVTADAPGALTAWAGPPRTGTPSARPGAGTAAPAELAATRHLLHHIA
                     RAPELPGGTWYPAPRPGPVTDGALEKRRSAPPDDLTHPPGEDLLSRLLTTARTARPEGP
                     GWTAAVGGDAPALYAKAPGSRGLDVRASGDARPTLARWAAGQQWIGGAGAVLLAHGCPG
                     DAPAAAVRTCHLAAGYAAGAVQAHATALGLRSRPIGSWQQADLGAALGEAPGRDWIVHG
                     LAVGGG"
     assembly_gap    7775..7784
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8030..9769
                     /locus_tag="MWG59_RS35950"
                     /old_locus_tag="MWG59_35950"
     CDS             8030..9769
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955801.1"
                     /locus_tag="MWG59_RS35950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_35950"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_248587351.1"
                     /transl_table=11
                     /translation="MMLHPELLRAASTARRPLGLATALLAAVTTTHLAQAVLLALVLAR
                     IARGETAALAPLLTAVIAVVLVRAGLGRAQRLAAVTAGATVRIGLRDTLLERLGALGPT
                     AITGARAGAVRATLVDGVEGVDAYVSRYLPQALITCAVPPMLLVAVALVEPYAALALGP
                     ALLLALFGPRWWDRLLARRGKEHWDSYEALAADYLEALQGMPTLRATGAVGRVRERLER
                     RSAALHRTTVAKLRVSLVDTGLTDLAVQGGTAAAVLVACSSAATGRTAATGTYLLLLLA
                     SECFRPVRDLSREWHAGYLGVSAADGIAGLRTAEAAVPDRGTVAADWARAPEIRFEDVH
                     FTHPGSGRPALDGVSFTAAAGRTTAVVGPSGAGKSTLLSLLLRQRDPDRGSLTVAGTAT
                     AAYTLASLRRGIAVVSQDTYLFHATIAENLRIARPAATDEQLRAAARTAGIDQEIARLP
                     DGYATLVGERGATLSGGQRQRLALARALLADAPVLILDEATSAVDERGQARIVRELSAA
                     GRGRTCVVVAHRLDAVRHADHIVVLDAGRVAATGDHTTLLAGGGVYASLVAAGRTAQEE
                     RAA"
ORIGIN
        1 acgccgccgg acctgcccgc ccccgtcctg cggaaggcgg aacgatgacg ctcatcgaga
       61 ccatgcccgg accggcccgt cgggccctat gtgcgccggg cgtccggatg gcctgtctgg
      121 cgctgctcgt cgccgcggtg gcctgcgccg atctgttcgc cgggaggggc gtcacccccg
      181 aaggcgtacg ggaggtgctg ctcggcggcg gcgaccccac cgccgagcac atcgtgctcc
      241 aactccggtt gccccggctg ctggtggcgc tggtcgccgg ggcctgcctc ggggtggccg
      301 ggctggtgct ccagtcggcc ctgcgcaacc cgctggccgg acccgaggtc accggcgtca
      361 cccccggtgc ggtgctcggc gcggtcaccg cgaccggcct cggcctcgcg ggttgggaat
      421 cccccacggc cgtggtcgtg gcggcctgcc tcggcggctt cgccggagcg ggcctgctgt
      481 ggctgctcgc cgggcgggag gcgggcgacc cggaacagac ggccgtctac ggggtcctcg
      541 tctcggcggt gctcgccggg ctcaccgcgg tcgtccttct ggtggccccc ggcgaactcg
      601 gcagcgttgt gcagtggttg atcggttcca ccgagggccg ggtgtggcag cactggaatc
      661 tgctctggcc gtgggccgtc ttctggggcg cggcggcctg gctgctcgcc ggcccgctga
      721 ccctgttgcg ctgcggcgac gaacaggcct ctgcggcggg cctggccacc ggccggggcc
      781 ggggtgcggc gctcgtctgc gccgtggcac tgacggccgg cgcagtgtcg gccgtgggcg
      841 cgctcggctt cgtggggctg ctggtgcccc atctggcgct ggccgtcttc ggggccgacc
      901 tgcgcatcac ccttccgggg gccgcgctgc tcggcgcgac ggtggtgtgc ggggcggacg
      961 ccgccgccca actgctgtcc cgactgctgg ccaccgcgct cgaagcggac cggctgaccc
     1021 tcccggtcgg cgccctgacc accttcgtgg gggcggggct gctgatggtc gtggcacggc
     1081 gacgggcgga cgagcgatga cggagagcgg tacgtggacg ggacacgccg atgtcggcga
     1141 cgccgtcgac tccgtcgacg gaagtggagg acaacggatg aggagggtgg catggcggaa
     1201 cgcctgaccg gacaggtgga gtcgagcccg gctgaggcgg ccgggccgga agcggaaccg
     1261 ggcgtgatgc ccggcccgga ggtcgtccgg gcggaggggc tggccttcgg ctacccggga
     1321 cgcatggtcc tgcgcggggt cgacctgagc gtccgcgccg gcgaactggt cgccctgatc
     1381 gggctgaacg gctgcggcaa gagcaccttt ctcaagctcg ccgccggact gctcgccccg
     1441 agcggcgggc gcgtcctgct ggaaggcgac gacgtgacgc ggcttccccg ccgcaccgcc
     1501 gcccggcgcg tcgccctgct ccatcagtcg gcgcccaccg tgccggggct gaccgtgcgg
     1561 caactcgtcc gacaggggcg ctatgccgcc cgggggccgc tcgggatgtt gcgggagggc
     1621 gacgacgagg tgacgtcccg ggcgctcgcg gacgtcggcg tcaccacctg ggccgaccgg
     1681 cccgtggacg cgctgtccgg aggcgaacgc cagcgggtcc ggctggccat ggcgctggcc
     1741 caggacgccc gcgtgctctt cctcgacgag ccgaccacct atctcgatct gcggcaccag
     1801 ttggaggtgc tccagacggt cgtacggctg cgctccgaac gccgcctgac ggtcgtgatg
     1861 gtgctgcacg acctcaacca cgccgctcgg ttcgccgatc ggatcgtcgc gctgcgcgag
     1921 ggtcgcgtcg ccgccgacgg acctccggcc gaagtggtca cccggcgtct gctggccgaa
     1981 gtcctgggcg tggagggccg ggtggggatc gacagcgagg gcggctggcc cgtctgttac
     2041 ccagatcacc ctctcgatcc gctccagcta ttgcggaatg aaaatcatat tcattagtgt
     2101 ctgtgctgcg gcacgcacca acgccgcgac cgaaacaccc atccgatccg accgaatgga
     2161 gacctcaact catggagaac aacaaggtgt tcagcccgat cgccgaccag ggtcagctcg
     2221 cccacctctc cgccacccac tcgaacgccc tcgtcgagaa ccccttcgac gacgcggtcg
     2281 aggccgacat cgcggccgag aagtaggcga cgcctgccgc tgacggcagg tttcccgccc
     2341 cgctcggccc ccgaacgttc cgtccctgct ttccgatccg gaggattccg tgacccgcag
     2401 tcgactcgct cccggtactg ggatcgtcac cgttcccggt ggcgcccccg tgctccgcac
     2461 ctccggaggg gagttcctgc gcatcgacac cggcggtgtc accggcgagg acctggtgcg
     2521 ccggctgacg ggggaggggg agccggagga ggagacaccg gacgagggcg cacacacggc
     2581 cgaactcacc cggctcgtcc aggcgttcga agccgccggg tacgcgctgt ccaccccgcc
     2641 gcgggccccg ctcgccggcc gcaccgtcca tgtcctcggc gaccccgcgc tcaccgcgcc
     2701 ggtggtgcgc tgcgccgggg cggagggggc cgaggtccac cggatcaccc cggacggtgt
     2761 ggcggagctg gcccgggcga ccccggacga aggcgccgaa gggcgcgtcg ccgtggtgtg
     2821 gtgcctcgac tcgcccgtgc ccgatgggct ctgggacacg gcggaccggc tgcccgggcg
     2881 gcgcatcgcc tggctgcgct gccaccgcga gggcgcccac agctggatcg aacccctcgc
     2941 cgcggccccg ggtgacatca cttcccgtca catccgtctc cgcagactcg cggctacccc
     3001 tgcccatcgc gaactcgccg cgtactgggc cggacaccgg acccccgaca ccggcccccg
     3061 ccccaccgaa gcctcggccg ccctgatcgc cgcgctcctc accgccgatc tgatcgcctg
     3121 ggccgaagcc gaagccgaag ccgaagccgg agagggagag ggagagggag agggagaagg
     3181 ggtgggggcc ggagccggga cgggtcgggc ggccgttctt ccggcgcgcc gtcggctccg
     3241 ccggatcgac ctgcgcgatc tcaccctcac cgagcacccc gtgctccccg tcccggacgt
     3301 cgctccccta cccgccccgg cggcggtgcg gacgcgtgcc gggaccgccg aggcccgcct
     3361 gtgagcaccc gtaccgtgta cgtccccacg ccggacggca ccgcgctcgc caccgacgta
     3421 tgcctcccgg acgggacccg cccccttccc gctgtcctga tcagaacccc ctacgggcgc
     3481 gaggcccacc gcaccgagct gcgcgcctgg gcggcgcacg gattcgccgc actcgcccag
     3541 gacgtatgcg gccgccacgg ctcaccgggg gagtggcacc cctaccggga ccacgagagg
     3601 acgaacggcg cggccaccgt cgcctgggtg cgcgcacagg cgtggagtaa cggggaggtc
     3661 gtcgccgtcg gcgcttccta cgccgcgtac tgcgccctcg tcaccgcact cgccacccct
     3721 gaagccgcct gcgatacggg ccccgacgcc gaccccggcg acttttaccg cgacggagtc
     3781 cccgacgccg tcatcgccgc cgtgcccgcg ctcgggctca ccgagacggc gcgcgagccc
     3841 ggtgggccgg agcggctgtg ggcccgggcc ggctggtggg cggcgcacgg cgaccgtcgc
     3901 gactccgacc cagacgcgct ggcccgcgcc ctggcggacg acccccggct gctcgaacac
     3961 ctcccggtcg cccgcctcgc ggagcggctg gccgcgagcc tggggcgcga actgccgtcc
     4021 tggtccgggc tctgggccga ccgcgcacga ggccgactcg tcgcgctggg ctccaccgcc
     4081 cgccttccgc tgctcgccgt cggcggcacc cgtgacccgt tcgccgagga caccgtggcc
     4141 ctgtggcgcg gctggggcgg accgtcccgt ctgctgctcg ggccctgggg ccaccgcctc
     4201 accgccgacc ccgccgcacc ggcacgcgca cgtgtcaacc tcggtgcgct gtacgtacgg
     4261 tgggcccgcg cggcgctctc cgggcggctg gagccgacac ggcacggggt catcgccctc
     4321 ggcgacagcg gccgctggca caccacccgg ctgcggagca ccccccgccc cgcgagcgcg
     4381 caggatgcca ccccacagga cgccaccgag cgggaggggg cggaccggcc ggaggcggag
     4441 agccgctggt ccttcgactc gcccaccggt ctgcggctcc tccacggagc cgagttcaac
     4501 gccgacccgg accgtcccgt gcgctccgac gacctcgccg tcccggccga cggcgatcgt
     4561 gccgaccgct gtctgctcct ctcgccgccg ctgccccgcc cgctcgacct ggcgggagcc
     4621 gccgtcgccc ggatcaacgt cacggccgac acgccctgtg ccgactgggc cgtacgcctg
     4681 accgcactcg accccaccgg ccgcgcggat ccgctcgctt tcggcatcgt ccggcgcacc
     4741 ggcccgcccg gcgaggccgc agacatcagc gtcccgctcg gcacactggg ccgtcggctg
     4801 cccgccggga cccgattgcg cgcggagatc gccggacacc acttccccgc ccacgcccgc
     4861 aacccgcaca ccggcgagga ccccgtcacc gccacccggc tcgccccgtc ccgccgcaca
     4921 gtggaccccc ggggcagcgc cctccacctg cccgtcgtcg cccgacgccg ctacgtcgag
     4981 cccgcaccgg agatatgccg ttgaccagcg ccctgcccat cgaagccctc gtggaccccg
     5041 tcagcgggat catccgcgac gtcgcgcccg tcgagcaccc caccggtgcg ccgccccgct
     5101 acaccgcgat gaccgccgac gtcgccgacg cccgccgtct cggcgcctgg ccagccgacc
     5161 gcgtctcgct cggcaccacg ttcggcgacc cccggggcgc gtggatcgcc gcggtggccg
     5221 aggccgtcga gcgctactgc ggcaaccgcc tcccaccgcc cggccacccc caggagcccc
     5281 gccgggccac cgccgccgaa ctggccgccg agggccaccg cctctacggt cccggcgctc
     5341 tgccggccta cgccccctgg cagtacgtcc gcccgggatt cccgtactcc gaactcactt
     5401 ccgaaacaga gacgttgtgg acccgggtca ccgagaatgg gcggccctgc tgggcccccg
     5461 tcgcgctgac ccacctcaac tggcggcagg gcgaactgcg ttcgctgccc cgcacccacc
     5521 acctcaacta tgcgggcatc gccaccgggc agggcctcga cgacgccgtc gagcgcggcc
     5581 tcctcgaagt cgtcgagcgc gacgccctgg agctgtggtg gcacctggac gggcccaccc
     5641 ggggcatcga tccggccagc gtccccggac tcaccgagga tctggccggc tgcgggctgg
     5701 acgtgcacat cgtggagatg ccctccgagt tcgccccctg tgtggcggcc ctcgcgcacg
     5761 atccggtacg cggcatatac gcggccgggt tcgcctgccg gtacgacccg gcggaggcgg
     5821 cccgcaaggc cgtgctggag gccgtgcaca cctgggtgtt cacccagggc gcggtggacg
     5881 ccgacggctg ggtgtaccgg gcggtcgaag ccgggatgct cgcccgtggc ctctacctcg
     5941 accaccgcgc cgaccggcgc tatctcgacg actgcgggcc cgagttcgcc gccgtccggg
     6001 acctcggtgc ccatgtccag gtctggctgg acgaccggat ggccccgctc gcccgacgct
     6061 tcaccgaacc cgctctcggc acggtcccgg tcaccgcgat cgaccccggc agccccaccg
     6121 cgctcgacac ggcgctcgcc gccggggggc accgggtgat caccgcggac ctcaccacgg
     6181 aggacgtcgc cgagacgagc ctgcgcgtcg cccgcgtact ggtctccggg ctgatcccca
     6241 acgcgcccgc ggccttcggc tacttcggct gcccgcgatt cgcggacgcc gcgctcgccc
     6301 ggggctggcg aacccgaccg cccgccgcac cgggggactt caccctcgcc ccgcccccgc
     6361 acatgtgaag gagcgcgccg ccatgcccga cacctcggcc cggaccgtcg cacccgaatc
     6421 cgggacgcgc cccacgtccg gcctcctgcg ccgggccctc gcagacgtcc gctcctcgcg
     6481 cgcgccgggc ctcgccccgt acgttcccga gcgcccgttc ccccggagcg gggcggggtt
     6541 gccgttggcc ggggacgagg cccggcccgg ggcgggtcca ggggccgggg gccgggccgc
     6601 gatcgacctg gaccgcgtcc tgcggctctc gctggccgcg cccgccgacg acaccgggcc
     6661 ggctgcgccc cgtcccctcg gcgggagccc tgcacccggt gcgcgcccac ctcctgatag
     6721 gtccggggtg ttcgctgccg cccggacgct acgcctacga cccgcgcgcc caccgcgccc
     6781 accggcgcgg cccggtgccc gacggcgtgt ccccgggtgt gctcgtcgtc ctcaccgtgg
     6841 ccgcgtcgcg caccgtcgcg cactacggcc accgcgcctg gccgctgctg ctgctggaca
     6901 cgggccacgc cgccgccgcc ctggcactgg ctcagccccc gccgccgacg tactggtctg
     6961 cctcgacgcg gacgccgccc tcctctccgc cgccgccgga ctgcccgccg ccccggaccg
     7021 gccggacacc tggcccgaag ccgagccgga actgccgctc gcagccgtca ggttgacgcc
     7081 aacgggcgtg accgccgacg cccccggggc actgaccgcc tgggccggcc cgccccgtac
     7141 cggaaccccg tcggcgcgac ccggcgcggg gaccgcagcg cccgccgaac tcgcggcgac
     7201 acggcacctg ttgcaccaca tcgcgcgagc ccccgagctg cccggcggca cctggtaccc
     7261 ggccccccgc ccgggaccgg tgaccgacgg ggcgctggag aagcgccgga gcgcaccgcc
     7321 cgacgacctg acccacccgc ccggagagga cctgctctcc cggctcctga ccaccgcgcg
     7381 gaccgcacgg ccggaggggc ccggctggac agcggcggtc ggtggtgacg ccccggccct
     7441 gtacgccaag gctcccggca gccgtggtct cgacgtccgc gcctccgggg acgcacgtcc
     7501 caccctcgcc cgctgggccg cgggccagca gtggatcggc ggggcgggcg cggtgctcct
     7561 cgcccacggc tgccccggtg acgccccggc ggccgctgtt cgcacctgcc acctcgccgc
     7621 cgggtacgcg gccggagcgg tccaggccca tgccaccgca ctgggcctgc gttcccggcc
     7681 catcggctcc tggcagcagg cggacctggg cgcggcgctc ggcgaggccc ccggacgcga
     7741 ctggatcgtg cacggcctgg ccgtgggggg ggggnnnnnn nnnnccccca ccgccgggcc
     7801 gtgtcgcccc gggcttcgtc cgacatccac cgacggccga aaggccgccc gcgccaacgc
     7861 cgcctgtcac gacggcaccc gcctcaacgg cacccgcccc gacgccaccc gcctcaacgc
     7921 cacccgcccc agaggcacgt ctcccgacgc cacctgcccc gacgccaccc gccacagcga
     7981 ccacgacacc cgccgccccg gcgactccgc ccggcgagga agaacgccca tgatgctcca
     8041 ccccgaactc ctgcgcgccg cgagcaccgc gcgccgcccc ctcggcctcg ccaccgcact
     8101 gctcgcggcg gtcaccacta cccacctcgc ccaggccgtc cttctcgccc tcgtgctcgc
     8161 ccggatcgcc cggggggaga cggccgccct cgccccgctg ctgaccgccg tcatcgccgt
     8221 cgtcctggtc cgcgccggcc tcggccgcgc gcagcgcctg gccgccgtca cggcgggagc
     8281 caccgtacgg atcgggcttc gcgacaccct gctggagcgg ctcggcgccc tcggccccac
     8341 cgcgatcacc ggtgcgcgtg ccggagctgt gcgcgccaca ctggtggacg gcgtcgaggg
     8401 agtcgacgcg tacgtctcgc gctatctgcc ccaagcgctc atcacctgcg ccgttccgcc
     8461 gatgctgctc gtcgccgtcg ccctcgtcga gccgtacgcg gccctcgccc tcggccccgc
     8521 cctgctgctc gccctcttcg ggccgcgctg gtgggaccgg ctgctcgcgc ggcgcggcaa
     8581 ggagcactgg gactcctacg aagccctcgc cgccgactac ctggaggcac tccaggggat
     8641 gccgaccctg cgtgccaccg gtgcggtggg ccgcgtacgg gaacggctgg agcggcggtc
     8701 ggccgccctg caccggacca ccgtcgccaa actccgggtg tcgctcgtcg acaccggcct
     8761 caccgacctg gccgtccagg gcggcacggc cgccgccgtt ctcgtggcgt gctcgtcggc
     8821 ggccaccggc cgtaccgccg ccaccggcac ctacctcctg ctgctcctcg cctccgaatg
     8881 cttccgcccc gtccgcgacc tctcccgcga gtggcacgcg ggctatctcg gggtgtccgc
     8941 ggccgacgga atcgccggcc tgcgcaccgc cgaggccgcc gtgcccgacc ggggcaccgt
     9001 ggccgccgac tgggcgaggg cacccgaaat ccgtttcgag gacgtgcact tcacccaccc
     9061 tgggagcggg cggcccgccc tcgacggcgt cagcttcacc gccgccgccg ggcggaccac
     9121 ggcggtcgtc ggtccgtccg gggccggtaa gtcgaccctg ctgagcctgc tgctgcggca
     9181 acgggacccg gaccggggca gcctcaccgt cgccgggacg gccaccgccg cgtacaccct
     9241 cgcctcgctg cggcggggca tcgccgtggt ctcgcaggac acctacctct tccacgccac
     9301 gatcgcggag aacctgcgga tcgcccgccc ggccgccacc gacgaacaac tgcgcgccgc
     9361 cgcccgcacc gcagggatcg accaggagat cgcccggctg ccggacggtt acgccaccct
     9421 cgtcggcgaa cgcggtgcca cgctctccgg cggacagcgc caacggctcg ccctcgcacg
     9481 ggcgttgctg gccgacgccc cggtcctgat cctggacgag gccaccagcg cggtcgacga
     9541 acgcggccag gcccgcatcg tgcgggaact ctccgcggcc ggccgaggac gcacgtgcgt
     9601 cgtcgtcgcc caccggctcg acgccgtacg ccacgccgac cacatcgtcg tcctggacgc
     9661 gggccgtgtc gccgcgacgg gcgaccacac caccctgctc gcgggcggcg gcgtgtacgc
     9721 ctcgctcgtc gcggccgggc gcaccgcaca ggaggagcgc gccgcatgac cccgaccccg
     9781 gccccgaccg atcccgcccg ttcccctgtc cccgccgccc ccgtccccgc cgccctgcgc
     9841 tcgctcctcc cggtcctcgg cgcgcaccgc gccaccgtgc tgcgtacctg cctggccgcc
     9901 ctcgtcgacc aggcggccct ggtcgccctc gtgaccctcg ccgcgtacac ggtgggcaca
     9961 gccgtcacgg agcaccgccc gcccaccacc gggaccgtcg ccgtcctgat cgggcttgtc
    10021 ctgctccggg ccctcgccac ctggcgggag atggacctct cgcacgacct cgcctaccgg
    10081 gtcctcgccg aactgcgcgt gcgggtcttc gacgggctgg cccgcagcgc ccccgcccgg
    10141 atcgcgggcc ggcgcagcgg cgacctcgcg gccaccgcgc tcggggacgt cgaggcactg
    10201 gagttcttct acgcccacgc catcgcccaa ctcctcgcct ccggtgtggt cttcgcggtg
    10261 tcggcggctg tgctggcggc cctcggcccc tggctgctgc tcgccgtcgt gcccgccgcg
    10321 ctcctcctga tctggtcgcc gctgttcgaa gcacgcgggc gcgcggaacg cggggcgcgg
    10381 acccggagcg cgctcgccga ggtctcgtcc gagaccgtgg agagcgtcga cgggctgcgc
    10441 gaactcctga tggcgggggc gctgaaccgc aggcgggcca ggctgcggtc cgccgggcgc
    10501 gggctcgccc gcgcccagcg cgccgaacag tcctgggaga ccggtgcggg cgcgacgcgc
    10561 gatctgctgg tcgtcgccgc cgtcatcggg gtcgtggcgg ccgccgccca cgccgcgtcc
    10621 gccggacgtc tggacggggc gtgggccccg gccgccatgg ccctggcgct gggagccctg
    10681 gccccggtcg ccgaatcggc ggccgcactc ggccgggcgg gcagtctgcg cgccgccgcg
    10741 gcacgggtcc gggccgccct gcacgccccc gccggagccc ccgcccccgc cgccccccgc
    10801 cccctaccgg cgggcccgct gggcctgcgg ctgcgcggag tccggttcgg ctacgggggc
    10861 gagccggtcc tggacggcct cgacctgacc gtccggccgg gcgagaccgt ggccctcgtc
    10921 ggagcgtccg gggcgggcaa gtcgacctgc gcacaccttc tggcgcggta ctgggacccc
    10981 gaggccggcg ccgtggagct cgttccccca gccccggctc accccgtgga cctgcgggac
    11041 ctcaccgagg aggacctgcg cgccgccgtg tccgtggtgg gccaggaggc cccgctcttc
    11101 cacggcaccc tcgccgagaa cctccgcctg ggcactccgg acgccacggc ggacgaactg
    11161 ggcgcggtga cccggatctg cggcatcgcc cccatagccg acgccctgcc cgacggtctt
    11221 gacaccgccg tcggcgagcg cggcgcgacc ctgtccggcg gccagcgcgc ccgggtggcc
    11281 ctggcccggg ggctgctggg cgggccgcgc gtactcgtcc tggacgagac gacggcccat
    11341 ctggaccacc ggggcgacgc ggagctgt
//
