LOCUS       NZ_CP096281            10798 bp    DNA     linear   CON 08-JUN-2024
DEFINITION  Streptomyces sp. WAC00303 chromosome.
ACCESSION   NZ_CP096281
VERSION     NZ_CP096281.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. WAC00303
  ORGANISM  Streptomyces sp. WAC00303
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8548838)
  AUTHORS   Meyer,K.J., Tan,S. and Nodwell,J.R.
  TITLE     Genome Sequence of WAC00303 Direct Submission
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8548838)
  AUTHORS   Meyer,K.J., Tan,S. and Nodwell,J.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Biochemistry, University of Toronto, 1 Kings
            College Circle 5207, Toronto, ON M5S 1A8, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-MAR-2022
            Assembly Method                   :: Flye v. 2.8.1
            Assembly Name                     :: WAC00303
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58.0x
            Sequencing Technology :: Oxford Nanopore; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023210535.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 05:21:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,823
            CDSs (total)                      :: 7,731
            Genes (coding)                    :: 7,293
            CDSs (with protein)               :: 7,293
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 438
            CDSs (without protein)            :: 438
            Pseudo Genes (ambiguous residues) :: 0 of 438
            Pseudo Genes (frameshifted)       :: 228 of 438
            Pseudo Genes (incomplete)         :: 241 of 438
            Pseudo Genes (internal stop)      :: 26 of 438
            Pseudo Genes (multiple problems)  :: 54 of 438
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:23:54
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7610884
            Orig. end                         :: 7621682
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096281.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Justin Nodwell, University
            of Toronto.
FEATURES             Location/Qualifiers
     region          1..10798
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="28"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10798
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10798
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5798](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5798)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            122..547
                     /gene="panD"
                     /locus_tag="MWG59_RS34770"
                     /old_locus_tag="MWG59_34770"
     CDS             122..547
                     /EC_number="4.1.1.11"
                     /GO_function="GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="panD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964661.1"
                     /locus_tag="MWG59_RS34770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_34770"
                     /product="aspartate 1-decarboxylase"
                     /protein_id="WP_050361929.1"
                     /transl_table=11
                     /translation="MLRTMFKSKIHRATVTQADLHYVGSVTIDAALMEAADLLPGELVH
                     IVDIDNGARLETYVIEGERGSGVIGINGAAAHLVHPGDLVILISYAQVDDAEARALVPR
                     VVHVDADNRIVALGADASAPVPGTRTERSPQAVVAGG"
     misc_feature    complement(126..140)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.95"
                     /tool="antismash"
     gene            609..962
                     /locus_tag="MWG59_RS34775"
                     /old_locus_tag="MWG59_34775"
     CDS             609..962
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955645.1"
                     /locus_tag="MWG59_RS34775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_34775"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_097931249.1"
                     /transl_table=11
                     /translation="MAEKAAVEIRDDREHGSLVAYEDGKPTGTIAYFVMDPAPGALVAV
                     HTVVRPEHEGKGIAGALVREFYTMAAREGVPVVPLCPYAAKWAQRHPDEAPEAPAELVR
                     EAERQLRNHPELF"
     gene            <963..1427
                     /locus_tag="MWG59_RS34780"
                     /old_locus_tag="MWG59_34780"
                     /pseudo=""
     CDS             <963..1427
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1097: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558180.1"
                     /locus_tag="MWG59_RS34780"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_34780"
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="RQRTGLPWRDLPPCFGSWKAVHDCHRRWSADGAWERILRAVQADA
                     EGRIDWSMVSVDSTSCRAHHRNRRHLRRRQIKHTIPEPRDQRANRRRRGSQGGRPTSFD
                     KTIYRRRNEVERTMNRLKNFRAVATRFDKRGYVFHGTVTVAAIRLWLRPQ"
     gene            complement(1533..1982)
                     /locus_tag="MWG59_RS34785"
                     /old_locus_tag="MWG59_34785"
     CDS             complement(1533..1982)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014044020.1"
                     /locus_tag="MWG59_RS34785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_34785"
                     /product="hypothetical protein"
                     /protein_id="WP_097931251.1"
                     /transl_table=11
                     /translation="MSRDAEVIVLARWSDEVMEPLTQDDPERTWRGRFVPIAGHWGYEF
                     GWALELEKVHARKGLLKHLESLPWPHPHTVQVLLRDQDDDCFGLWMFHEGRLVEVTLAR
                     TERFHQPAPPNEDFEPDPGMLLRTDQNTALPEQTPEACRDARSPW"
     gene            complement(2270..3040)
                     /locus_tag="MWG59_RS34790"
                     /old_locus_tag="MWG59_34790"
     CDS             complement(2270..3040)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PIG-L"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955646.1"
                     /locus_tag="MWG59_RS34790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_34790"
                     /product="PIG-L family deacetylase"
                     /protein_id="WP_248586679.1"
                     /sec_met_domain="PIG-L (E-value: 2.8e-21, bitscore: 75.4,
                     seeds: 137, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPLPSVLAVHAHPDDESLFCGGVLAQHAAAGARTAVVTATWAEGT
                     HRAVELAGALAALGAGAPRLLGYADARVPESAPGRPRFLDAPLDDAVEAVVSHIRDVRP
                     EIVITVDAYGGMTGHPDHVHAHRITALAVRAAGLPRFCPGSGDPWQPSALHLATHPRSA
                     AVPVGGRLARPGTPADALYCSEDAWITTTVDVGPWFPQKLAAILAHRSEVERGAAPGRI
                     AALPQAVQREVLGTEWYIRHDLAPRSGSATELSA"
     gene            complement(3527..4909)
                     /locus_tag="MWG59_RS34795"
                     /old_locus_tag="MWG59_34795"
     CDS             complement(3527..4909)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_DAP_Arg_deC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264: diaminopimelate decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955647.1"
                     /locus_tag="MWG59_RS34795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_34795"
                     /product="diaminopimelate decarboxylase"
                     /protein_id="WP_248586680.1"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 7.4e-37, bitscore:
                     125.9, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 5.4e-08,
                     bitscore: 31.8, seeds: 500, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQTTSAHHQGGTAPPAARFDAVVRAAVGQGLLGEGCPVAGFIDT
                     EGVRASVGALHDAFAATPEVLHTFAAKACSLVPVLRLLADCGMGCEVASPGELRLAVEA
                     GFAASRVVLDSPAKTREEIRLALALGVAVNADSLDELRRIDALRNPGSASVLGLRVNPQ
                     VGGGSIGPMSTATDTSKFGVALRDPGVREAIVEAFRKRPWLTRLHTHVGSQGCPLELIA
                     DGVARTYELAEEINATLGVRRVTSLDIGGGLPVNFADETVRPGYADYVAALTAAAPGLF
                     SGRYSLVTEFGRSLLAKNGFIGARVEYTKDAGGRRIALTHAGAQTATRTVLMPDAWPLR
                     IGAFGPDGTPKTGPSLAQDIAGPCCFAGDVVAYGRELPELAEDDVVVLYDTGAYYFSTP
                     WAYNSLPRPAVYGFRAVAGPEDGSGHDGPVTFATVRDAQSLDDIAAESGLAHADALIRR
                     DG"
     gene            complement(5001..5798)
                     /locus_tag="MWG59_RS34800"
                     /old_locus_tag="MWG59_34800"
     CDS             complement(5001..5798)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955648.1"
                     /locus_tag="MWG59_RS34800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_34800"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_097935861.1"
                     /sec_met_domain="Linocin_M18 (E-value: 3.5e-88, bitscore:
                     293.8, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPVTPSAWDEIEEEARRTFRRHVAGRRVVDVSDPDGP
                     TLAAVGDGHLTDIDPPTPDVVARARTSMPVIEWRVPFTVTRRAVDDVERGSADSDWQPV
                     KDAARTCAFAEDMAIIDGYAAAGITGLRDGSSHDPLPLPADARDYPVAVSQAVTRLRLA
                     GVDGPYRLLLGADAFTEAAETSDHGYPVKTHLSRLVDDEILWAPAVKGGVLLSARGGDF
                     ELCLGQDLSIGYADHDATSIHLYFQQAFTFRMLTPEAVVGLIA"
     gene            complement(5798..6871)
                     /locus_tag="MWG59_RS34805"
                     /old_locus_tag="MWG59_34805"
     CDS             complement(5798..6871)
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456253.1"
                     /locus_tag="MWG59_RS34805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_34805"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_097935864.1"
                     /transl_table=11
                     /translation="MPDAVPDPVSVQPVVAPLTSAALFLVGTIEPGGESAVRDVLPDLA
                     AVARSLGFRYPDAGLACVAGFGSSAWDRLFGGPRPAHLHPFPELRGPLHHAPATPGDVL
                     LHIRAERMDVCYAWAAQLLDKLGGALRIVDETHGFRYLDHRDLLGFVDGTENPVGDDAR
                     AAALVGAGDDPDFEGGSYVVVQKYLHDLASWNALSVEEQERVIGRTKLDDVEFPDDEKP
                     ADSHLALNTITDPDGTERDILRANMPFGSFGKGEFGTYFIGYAADPDVTERMLRNMFLG
                     DPPGTHDRILDFSTAVTGSLFFAPRADFLDAPPPPPVLTSSGDRPEPVPAPVRQEPVTA
                     GADHGSLRIGSLQESAQ"
     gene            complement(7019..7648)
                     /locus_tag="MWG59_RS34810"
                     /old_locus_tag="MWG59_34810"
                     /pseudo=""
     CDS             complement(7019..7648)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122776.1"
                     /locus_tag="MWG59_RS34810"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_34810"
                     /product="TetR/AcrR family transcriptional regulator"
                     /pseudo=""
                     /transl_table=11
                     /translation="MPQASTYHHGDLRAACLRAARELLEEDGSAALSLRAVARRAGVSA
                     TAPYRLRRP"
     gene            complement(7712..8686)
                     /locus_tag="MWG59_RS34815"
                     /old_locus_tag="MWG59_34815"
     CDS             complement(7712..8686)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1066: alpha/beta hydrolase domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018100029.1"
                     /locus_tag="MWG59_RS34815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_34815"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_097935869.1"
                     /transl_table=11
                     /translation="MTDLPPPITPYLEPAAKELCEATDPHPRIYEVPPEKGRDILLGLQ
                     SDRSVPRPEVDEEWVDVDAGQWGTVRTRIIRPKGATGQLPVVFYIHGAGWVFGDDKTHD
                     RLFRELSVGAGAAGVFPVYDRAPEAKYPTQVEQNYAVGQWVLEHGAQHGLDTSRIAVTG
                     ESVGGCMSAVFALMNKERGGIDLKAQVLLYPVADADFNTPSYLQFAEGYYLTRDGMKWF
                     WDAYTGNLAHRTEAYASPLRSSLEQLRGLPTTLVITDEADVLRDEGEKYANRLREAGVD
                     VTSVRVAGMVHDFLLLDSLRDTRAANVARKLAVEALRTALHDD"
     misc_feature    8693..8703
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.79"
                     /tool="antismash"
     gene            complement(8944..10383)
                     /gene="gndA"
                     /locus_tag="MWG59_RS34820"
                     /old_locus_tag="MWG59_34820"
     CDS             complement(8944..10383)
                     /EC_number="1.1.1.44"
                     /GO_function="GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_process="GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="gndA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1100: 3-hydroxyisobutyrate dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007389208.1"
                     /locus_tag="MWG59_RS34820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_34820"
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="WP_248586681.1"
                     /transl_table=11
                     /translation="MSGTAQIGVTGLAVMGRNLARNFARNGYTVALHNRTVARTHELVE
                     EFGDEGDFIATESAQDFVAALERPRRLVIMVKAGEATDAVIAEFAPLLETGDVIIDGGN
                     AHFEDTRRRERELREQGIHFVGTGISGGEEGALEGPSIMPGGSEESYDSLGPMLEKISA
                     KAKDGAPCVTHIGPDGAGHFVKMTHNGIEYADMQLIGEAYQLLRDVAGYSPAQIADIFR
                     TWNTGRLDSYLIEITAEVLSHVDASTGEPFVDVVLDQAEQKGTGRWTVQIALDLGVPVS
                     AIAEAVFARSVSGHADLRAASRHLTGPTARRLGADEAAAFADQVEQALYASKIVAYTQG
                     FHEIAAGSERYDWNIDLGEVASIWRGGCIIRAAFLDRITSAYEARPRLPSLLADKSFAE
                     EIAAAQGDWRTVIATAVTRGVPTPAFAATLAYYDSLRAERLPAALTQGQRDYFGAHTYH
                     RTDREGTFHTLWGGDRTEVES"
ORIGIN
        1 gccgtctcgc ccggccgaag cgatgttcct cacaaacacc gttcggcgcg tgccggtgta
       61 aagtcgaggg cgcccttgac ctgcgcaaag caggcaggga gccgtctcgg aggagtccca
      121 agtgctgcgc acgatgttca agtccaagat tcaccgcgcc accgtcaccc aggccgacct
      181 gcactacgtc ggttccgtga cgatcgacgc cgccctgatg gaggccgccg acctgctgcc
      241 cggcgagctg gtccatatcg ttgacatcga caacggcgcc cgcctggaga cgtacgtcat
      301 cgagggcgag cgcggctccg gtgtcatcgg gatcaacggc gcggccgcac acctcgtgca
      361 ccccggtgac ctggtcatcc tgatcagcta cgcacaggtc gacgacgcgg aggcccgtgc
      421 cctcgtgccg cgggtcgtgc acgtggacgc ggacaaccgg atcgtggcgc tcggcgccga
      481 cgcctcggcg ccggtccccg ggacccgtac ggagcggagc ccgcaggccg tcgtcgcggg
      541 cggctgaggt cgccgtccgt gcttccggac gggtgacgtc cacgtccacg aacgagaagg
      601 caggggccat ggccgagaag gcagcggtag agatcaggga cgaccgggag cacggcagtc
      661 tcgtggcgta cgaggacggc aagccgaccg ggaccatcgc gtacttcgtc atggacccgg
      721 cccccggggc gctggtggcc gtgcacaccg tcgtccggcc cgaacacgag ggcaagggca
      781 tcgcgggtgc gctcgtccgg gagttctaca ccatggcggc ccgcgaggga gtccccgtcg
      841 ttccgctgtg cccgtacgcg gcgaagtggg cgcagcgtca ccccgacgag gcgcccgaag
      901 caccggccga gctcgtccgg gaggcggagc ggcagctcag gaaccacccc gagctgttct
      961 gacggcagcg gaccggcctg ccctggcggg acctgcctcc ctgcttcggt agttggaagg
     1021 cggttcacga ttgtcatcgc aggtggtcgg cggacggcgc gtgggagagg atcctgcggg
     1081 ccgtccaggc cgatgccgag ggccggatcg actggagcat ggtcagcgtc gattccacga
     1141 gctgccgcgc tcatcaccgt aatcggcgtc acctgcgcag acgccagatc aagcacacga
     1201 tccccgagcc gagagaccaa cgggccaacc gccggcgccg cggcagccaa ggaggccgac
     1261 ccacaagttt cgacaagacg atctaccgcc gccgcaacga ggtcgagcgg accatgaacc
     1321 ggctcaagaa cttccgagcc gtcgccacgc gcttcgacaa acgggggtac gtcttccacg
     1381 ggacggtcac cgtggccgcg atccgcctat ggctccgccc gcagtgaagc gccgcatctg
     1441 agcgtttcgg aacctgggca gtgatggcgc agttcgccga gtgggcccgc ctcgacagcc
     1501 ctgacgtccc acgatgagct gtcgaactcg ctctaccagg gtgagcgggc gtcgcggcat
     1561 gcttccgggg tctgctcggg cagagccgtg ttctggtcgg tgcgcagcag catgcctggg
     1621 tcgggttcga agtcctcatt gggcggtgct ggctggtgga aacgctcggt gcgcgcaagg
     1681 gtgacttcca ccagtcggcc ctcgtggaac atccatagcc cgaagcagtc gtcgtcctgg
     1741 tcgcgaagca gaacttgcac cgtatgcggg tgcggccacg gcagcgactc caggtgcttg
     1801 agcaggccct tgcgggcatg cactttctcc aactccaggg cccacccgaa ctcgtagccc
     1861 caatgccccg ctatcgggac gaaacggcct cgccatgtgc gttccgggtc gtcctgggtc
     1921 agcggttcca tcacttcgtc cgaccagcgg gcgagcacga tgacctcggc gtctctactc
     1981 atgccggagt atcaccgcat ccgcctgatc caccgcaacc gaattcctca accagccgac
     2041 gcaggcaagg gtgggcggga ttggcggcaa aaccccagtt ccagcgagcc cgacgacgac
     2101 gccttcgggg aagctcatct gcgcgtaagc cacgactctg atcagctcac acgacccatc
     2161 ggacacgccc taggggtggg gagtcgccgt agcggccgtt gcggaatcgc ggccgctgcc
     2221 cgtgcagggg ctaccggccc ggccgccccc gggcaaggcc tggggcatct cacgcgctca
     2281 gttccgtggc cgacccgctc cggggtgcga ggtcgtggcg gatgtaccac tcggtgccca
     2341 gcacctcgcg ctgaactgcc tgaggcaggg cggcgatccg gccgggcgcc gctcctcgtt
     2401 ccacctcgct gcggtgcgcc aggatcgcgg cgagcttctg cggaaaccag gggcccacgt
     2461 cgacggtcgt ggtgatccac gcgtcctccg agcagtacag cgcgtcggcc ggggttcccg
     2521 gccgggccag ccgcccgccc accgggacgg ccgccgagcg ggggtgcgtg gcgaggtgga
     2581 gggcgctcgg ctgccaggga tcaccggatc ctggacagaa acgggggagg ccggcggccc
     2641 ggaccgcaag cgcggtgatc cggtgggcgt gtacgtggtc ggggtggccg gtcatgcctc
     2701 cgtaggcgtc caccgtgatc acgatctccg gccggacgtc acggatatgg ctgacgacgg
     2761 cctccacggc atcgtccagc ggcgcgtcga ggaaccgggg ccggccgggg gccgactccg
     2821 gcacacgggc gtccgcgtag ccgagcagtc gtggggcgcc cgcgcccaga gcggcgaggg
     2881 ccccggcgag ctcgacggcc ctgtgcgtgc cctccgccca ggtcgccgtg accaccgcgg
     2941 tgcgggctcc ggcggccgcg tgctgggcga ggacgcctcc gcagaacagg gactcgtcgt
     3001 ccggatgggc gtggacggcg agaacgctcg gcaggggcat gggggacacc tttcgcatcg
     3061 acggcgccgg tcgcggatcc ggacggatcc ggtcaggtcc gggcggatcg caggagttct
     3121 gcccactccg gacggcccgg cacacgtcgg cccgcgtggc tgtcgcccgg cgcaggtgtg
     3181 gactcggcag gcgcgggcag ccgctccggg agacggtgga ggaaagctcc atctgtccat
     3241 ttcatgccgt tgtggaggaa cgtgatgacc cacccgtacc ccgaccccgt accgccctcg
     3301 ccgacgcctc cgcccgtacc gggcccgccc actccggtgc cgggcccgcc gcccgtgccg
     3361 ccgggcccgg ggccggtacc gccgggtccc gcgccggacc cgatcccgca gccgcccgcg
     3421 ccgggcccgg accccgtacc gggtccggag cccgagccgc agcccgccgg gacgtgaccg
     3481 gcggccgggt ccggttccgg ccggtgccag ggcccggccc gcccgttcag ccgtcccgcc
     3541 ggatcagcgc gtccgcgtgc gcgaggccgc tctccgccgc gatgtcgtcc agcgactgcg
     3601 cgtcccgcac cgtcgcgaaa gtcaccggtc cgtcgtgccc cgacccgtcc tccggccccg
     3661 ccaccgcgcg gaatccgtac accgcgggac ggggcaggct gttgtacgcc cacggggtgg
     3721 agaagtagta cgcccccgtg tcgtacagca cgaccacatc gtcctcggcc agctccggca
     3781 gctccctgcc gtacgccacc acgtcccccg cgaagcagca cggccccgcg atgtcctgcg
     3841 ccagcgacgg cccggtcttg ggagtgccgt ccgggccgaa cgccccgatc cgcaagggcc
     3901 aggcgtcggg catgaggacg gtacgggtgg cggtctgggc tcccgcgtgg gtgagtgcga
     3961 tccggcggcc cccggcatcc ttcgtgtact ccacccgggc cccgatgaac ccgttcttcg
     4021 ccagcagcga ccggccgaac tcggtgacca gggagtagcg gccggagaag aggccggggg
     4081 cggccgccgt cagggccgcc acgtagtccg cgtaaccggg gcgcaccgtc tcgtcggcga
     4141 agttgaccgg gaggccaccg ccgatgtcca ggctggtgac ccgccggacg cccagcgtgg
     4201 cgttgatctc ctcggccagc tcgtacgtcc gtgccacccc gtccgcgatc agctccaacg
     4261 ggcagccctg ggagcccaca tgggtgtgca gccgggtcag ccacggccgc ttccggaagg
     4321 cctccacgat cgcctcgcgg acccccgggt cgcgcagcgc gaccccgaat ttcgacgtgt
     4381 ccgtcgccgt gctcatcggc ccgatcgagc caccgcccac ttgagggttg acccgcagtc
     4441 cgaggaccga ggcgctgccc gggttacgga gggcgtcgat gcgccgcagt tcgtcgaggc
     4501 tgtcggcgtt gacggccacg ccgagggcga gcgccagccg gatctcctcg cgggtcttgg
     4561 cgggggagtc caggacgaca cgggacgcgg cgaacccggc ctccacggcc agccgcagtt
     4621 caccggggct cgccacctcg catcccatgc cgcagtcggc gagcagccgc agcaccggga
     4681 cgagggagca ggccttggcc gcgaaggtgt gcagcacctc gggcgtggcg gcgaaggcgt
     4741 cgtgcagggc gccgacggag gcgcgcacgc cctccgtgtc gatgaatccg gcgaccgggc
     4801 agccctcgcc cagcagcccc tggccgacgg ccgcccggac gacggcgtcg aagcgggctg
     4861 ccggaggtgc cgtgccgccc tggtggtgcg cggaagtcgt ctgcgtcata cggccagagt
     4921 ccctgatggc tgcccccagg tcttcggcgg aacgggggcg ctcggtcgcc cggccacgcg
     4981 ccggtgagcg cccccgggga tcaggcgatc aggccgacca cggcctccgg tgtcagcatc
     5041 cggaaggtga aggcctgctg gaagtagagg tggatgctcg tggcgtcgtg gtccgcgtag
     5101 ccgatcgaca ggtcctggcc caggcagagt tcgaagtccc cgccccgggc cgacaggagc
     5161 accccgccct tgaccgcggg cgcccacagg atctcgtcgt ccaccaggcg gctcaagtgc
     5221 gtcttgaccg gatagccgtg gtcggacgtc tccgcggcct cggtgaacgc gtccgcgccg
     5281 agcagcagcc ggtacgggcc gtcgacaccg gccagccgca gtcgcgtcac ggcctggctg
     5341 accgccaccg gatagtcccg cgcgtcggcg ggcagaggca gcggatcgtg cgaggagccg
     5401 tcccgcaggc cggtgatccc ggccgccgcg tacccgtcga tgatcgccat gtcctcggcg
     5461 aacgcacagg tgcgtgccgc gtccttgacg ggctgccagt cgctgtcggc ggagccgcgc
     5521 tccacgtcgt ccacggcccg gcgggtcacc gtgaacggca cccgccactc gatgaccggc
     5581 atcgacgtgc gggcccgtgc caccacgtcc ggggtgggcg gatcaatatc ggtcagatgg
     5641 ccgtcgccga cggcggccag cgtcggaccg tccggatcgg acacgtcgac gacacggcgg
     5701 ccggcgacgt ggcggcggaa ggtgcgccgt gcctcctcct cgatctcgtc ccaggcggac
     5761 ggggtgaccg gagcgagttc gcgatgcaga ttgttcatca ctgggcgctt tcttgcaggc
     5821 tgccgatgcg cagcgaacca tggtccgccc cggccgtgac aggctcctgc cggaccggtg
     5881 cgggcaccgg ttccggccgg tccccggagc tggtcaggac gggcgggggc ggcggagcgt
     5941 cgaggaagtc ggcgcggggg gcgaagaaga gggaaccggt gaccgccgtg gagaagtcga
     6001 ggatgcggtc gtgcgtcccg ggcggatcgc cgaggaacat gttgcggagc atccgctcgg
     6061 tcacgtcggg atcggcggcg tacccgatga agtacgtacc gaactcgccc ttcccgaagc
     6121 tcccgaacgg catgttcgcc cgcaggatgt cccgttcggt gccgtccggg tcggtgatcg
     6181 tgttgagcgc gaggtgggag tcggcgggct tctcgtcgtc ggggaactcc acgtcgtcca
     6241 gtttggtgcg gccgatgacg cgttcctgct cctccacgga gagcgcgttc caggacgcca
     6301 ggtcgtgcag gtacttctgt acgacgacgt agctgccgcc ctcgaagtcc gggtcgtccc
     6361 cggccccgac gagcgccgcc gcacgggcgt cgtcgcccac cgggttctcc gtaccgtcca
     6421 cgaagccgag caggtcccgg tggtccaggt agcggaagcc gtgcgtctcg tccacgatcc
     6481 gcagggctcc gccgagcttg tcgagcaact gggccgccca cgcgtagcag acgtccatcc
     6541 gctcggcccg gatgtgcagc agtacgtcgc cgggggtcgc cggggcgtgg tgaagaggcc
     6601 ctcgcagctc cgggaacggg tgcaggtgag ccgggcgtgg cccgccgaac agccggtccc
     6661 aggcgctgga gccgaagccc gccacacagg cgagaccggc gtccgggtac cggaaaccga
     6721 gcgaccgggc gacggccgcg agatccggca gcacgtcccg caccgcgctc tcgccgcccg
     6781 gctcgatcgt gccgaccagg aacagcgccg cgctggtcaa cggcgccacg acaggctgga
     6841 cggagacggg atcagggacg gcgtcgggca tgcgcgcact ccattacgac ggtgtacgga
     6901 cggcggtgag cagccggcgg tccacggacc ccgacgaaca gggctcgcag ccactaaccc
     6961 acgtcccgtc cccggccgca cgccgggagc gcccggccgg gggagcgcgc ggccccggtc
     7021 aggatccgcc gggcacgccc tggtcgccgg ccggtgcggc agccgcccgg gacatcgcgg
     7081 gggaggcgtc gaacagtgcg tggacggcgg attcgacctg ggcggcgacg acctcggggg
     7141 tggagatgtc gagcttgccg tcgaggtgcg ggaacgcgag accgtggacg agggcccgca
     7201 ccgtggtcga cagggcgtcc gcgtccacgc cggggaaggc ggcgcggacg atgccgcgga
     7261 cgtactccga gatggcggcg gtggcggcga cccgttcttc gctggtcggg tcgcagtgcc
     7321 cggcgaacat cgcccggaac agggccgggt gatcgagtgc gaaccggacg tgggcgaccg
     7381 cgacctcggc gagtccgtcg ggcgtcgagg gggcgggatg ggcccggcca ggtgtccggc
     7441 gagctcgccg tacccctcgg cggccaccgc ggagacgaga gcgtcacggt cggcgaaggc
     7501 ggtagggggc cgtcgccgac actccggcgc gccgggcgac cgcgcgcagc gaaagggcgg
     7561 cgctgccgtc ctcctccagg agttcgcgtg cggcacgcag gcaggcggcg cgcagatccc
     7621 cgtggtggta cgtgctggcc tgcggcacag gtcacacccc tcgaagttca cggtgctcac
     7681 ctgcccgggc cgtcggcgcg gcggggcggc ctcagtcgtc gtgcagtgct gttcgcaggg
     7741 cttcgacggc gagcttgcgg gcgacgttcg cggcgcgggt gtcgcgcagg ctgtccagga
     7801 ggaggaagtc gtggaccatg cccgccacgc ggacggaggt cacgtccacg cctgcctcgc
     7861 gcagcctgtt ggcgtacttc tcgccctcgt cgcgcaggac gtcggcctcg tcggtgatga
     7921 ccagcgtggt gggcaggccc cggagctgct ccagggagga ccggagcggt gaagcgtatg
     7981 cctccgtgcg gtgggcgagg ttgccggtgt aggcgtccca gaaccacttc atgccgtccc
     8041 gggtgaggta gtagccctcg gcgaactgga ggtacgaagg agtgttgaag tcggcgtcgg
     8101 cgaccgggta cagcaggacc tgcgccttga ggtcgatgcc gccgcgctcc ttgttcatca
     8161 gcgcgaagac ggcggacatg cagccgccga ccgactcgcc ggtgacggcg atccgggagg
     8221 tgtccaggcc gtgctgcgcg ccgtgttcca gcacccactg gcccaccgcg tagttctgtt
     8281 cgacctgggt ggggtacttc gcctcgggtg cgcggtcgta gacggggaag acgcccgccg
     8341 ccccggcgcc cacggagagc tcgcggaaga ggcggtcgtg cgtcttgtcg tcgccgaaga
     8401 cccagccggc accgtggatg tagaacacca ccggcagctg tccggtggcc cccttgggcc
     8461 ggatgatgcg ggtgcggacc gtaccccact ggcccgcgtc cacgtcgacc cactcctcgt
     8521 cgacctcggg acggggcacg ctccggtcgc tctgcaggcc gagcaggatg tcgcggccct
     8581 tctccggggg gacctcgtag atccgcggat gcgggtccgt ggcctcgcac agttccttcg
     8641 cggcgggttc gaggtaaggc gtgatcggag gaggaagatc ggtcatgtcg gctcccatgt
     8701 cacgggcgcg cgggcaccct gttcacgcgg cttcgcgggc cgccgctgct gttcgccccc
     8761 gggtccgccc cctcgttcca tcctcgcccc ccgcccgtgg agcgcaaccg cgccgggcgc
     8821 ttcggatgtt gacgccgcac acgccggtac ttgatgtatg ccttgcgaac attgaggtcg
     8881 cgggtacgcg ggcatcctca cccacccggt cggatcgacc ggagcgcgag tctcgaaggc
     8941 gcgtcagctt tcgacctcgg tcctgtcgcc gccccagaga gtgtggaacg tgccctcgcg
     9001 gtcggtgcgg tggtaggtgt gtgcgccgaa gtagtcgcgc tggccctggg tgagggcggc
     9061 ggggaggcgt tcggcgcgga gcgagtcgta gtaggcgagg gtggcggcga aggccggggt
     9121 ggggacgccc cgggtgacgg cggtggcgat cacggtgcgc cagtcgccct gtgcggcggc
     9181 gatctcctcc gcgaaactct tgtcggcgag gaggctgggc agcctcggcc gggcttcgta
     9241 ggcgctggtg atcctgtcga ggaacgcggc ccggatgatg catccgccgc gccagatcga
     9301 ggcgacctcg ccgaggtcga tgttccagtc gtaccgctcg ctgccggccg cgatctcgtg
     9361 gaagccctgg gtgtaggcga cgatcttcga ggcgtacaac gcctgctcga cctggtcggc
     9421 gaatgccgcc gcctcgtccg cgccgagcct gcgggcggtc ggtccggtga ggtggcgtga
     9481 ggcggcacgg aggtcggcgt ggccggagac ggagcgggcg aagacggcct cggcgatggc
     9541 ggagacggga acgcccaggt cgagggcgat ctgcacggtc cagcggccgg tgcccttctg
     9601 ctccgcctgg tcgagcacga cgtccacgaa gggttcgccg gtcgacgcgt cgacatgtga
     9661 gaggacttcc gcggtgatct cgatgaggta ggagtccagg cgcccggtgt tccaggtgcg
     9721 gaagatgtcc gcgatctgcg cgggggagta tccggccacg tcgcgcagga gttggtaggc
     9781 ctcgccgatc agctgcatgt ccgcgtactc gatgccgttg tgtgtcattt tcacgaagtg
     9841 cccggcaccg tccggcccga tatgtgtgac gcagggagca ccgtccttgg ccttcgcgga
     9901 gatcttctcc agcatcggcc cgagtgagtc gtacgactcc tcgctgccgc ccggcatgat
     9961 gcttggtcct tcgagcgcgc cctcctcgcc tccggagata ccggtgccga cgaagtggat
    10021 gccctgctcg cgcagctcgc gctcccggcg gcgcgtgtcc tcgaagtgcg cgttgcctcc
    10081 gtcgatgatc acatcgccgg tttccaggag aggcgcgaac tccgcgatca ccgcgtccgt
    10141 ggcctccccg gccttcacca tgatgaccag gcgtcgtggc ctttccaggg ccgccacgaa
    10201 atcctgggcg ctctcggtcg cgatgaaatc accctcgtcg ccgaactctt cgacgagttc
    10261 atgggtgcgg gcgaccgtgc ggttgtgcag agcgacggtg tatccgttgc gggcgaagtt
    10321 ccgggccagg ttgcgtccca tgacggcaag tccggtgacg ccgatctgag cagtgccgct
    10381 catctgtgtg ctcctgggga tccgagtacg tcggtgcgtc cggtgcgatc ggaggtgcgt
    10441 ggtgcgttgg gcggtgcgtg gggtgcttcg ctcaggggag tacgggcccg gggacggccg
    10501 cgttcaggag tgggggctcg ccgacggtcc gctcagggcg agggcgaggc tgccgcgcca
    10561 ccggtggccg ggccggtccc ggtggccgga ccggcctcca ggagcgcttc gtcgtccccc
    10621 ggctcgggga tcacgtgcat cgcggcctcc tcggccgacg ccgccgcccc gtcgatgccc
    10681 acgtcgctgg cggtgagggt ggccggggcg cttccgtccg cctgctgggt cagccgcccg
    10741 gcgcgggcca cgccgacctc gtcgtcccag ggctcgccgt cgccgcccac cagatcgc
//
