LOCUS       NZ_CP096281            11329 bp    DNA     linear   CON 08-JUN-2024
DEFINITION  Streptomyces sp. WAC00303 chromosome.
ACCESSION   NZ_CP096281
VERSION     NZ_CP096281.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. WAC00303
  ORGANISM  Streptomyces sp. WAC00303
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8548838)
  AUTHORS   Meyer,K.J., Tan,S. and Nodwell,J.R.
  TITLE     Genome Sequence of WAC00303 Direct Submission
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8548838)
  AUTHORS   Meyer,K.J., Tan,S. and Nodwell,J.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Biochemistry, University of Toronto, 1 Kings
            College Circle 5207, Toronto, ON M5S 1A8, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-MAR-2022
            Assembly Method                   :: Flye v. 2.8.1
            Assembly Name                     :: WAC00303
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58.0x
            Sequencing Technology :: Oxford Nanopore; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023210535.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 05:21:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,823
            CDSs (total)                      :: 7,731
            Genes (coding)                    :: 7,293
            CDSs (with protein)               :: 7,293
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 438
            CDSs (without protein)            :: 438
            Pseudo Genes (ambiguous residues) :: 0 of 438
            Pseudo Genes (frameshifted)       :: 228 of 438
            Pseudo Genes (incomplete)         :: 241 of 438
            Pseudo Genes (internal stop)      :: 26 of 438
            Pseudo Genes (multiple problems)  :: 54 of 438
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:23:54
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6700014
            Orig. end                         :: 6711343
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096281.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Justin Nodwell, University
            of Toronto.
FEATURES             Location/Qualifiers
     region          1..11329
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="23"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11329
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11329
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6329](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6329)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    351..366
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.74"
                     /tool="antismash"
     misc_feature    complement(902..918)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.3"
                     /tool="antismash"
     gene            1353..2081
                     /gene="hemQ"
                     /locus_tag="MWG59_RS30765"
                     /old_locus_tag="MWG59_30765"
     CDS             1353..2081
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965515.1"
                     /locus_tag="MWG59_RS30765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_30765"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_026290345.1"
                     /transl_table=11
                     /translation="MSAPETVTSSKSPNAGKKAKDLNEVIRYTLWSVFKLRDVLPLDRA
                     GYADEVQELFDQLAAKDITVRGTYDVSGLRADADLMIWWHAETSDELQEAYNLFRRTKL
                     GRALDPVWSNMALHRPAEFNKSHVPAFLADETPRDYISVYPFVRSYDWYLLPDEDRRRM
                     LADHGKMARGYPDVRANTVASFSLGDYEWVLAFEADELYRIVDLMRHLRASEARLHVRE
                     EVPFYTGRRKSVADLVAGLA"
     assembly_gap    2187..2196
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2231..3793)
                     /locus_tag="MWG59_RS30770"
                     /old_locus_tag="MWG59_30770"
     CDS             complement(2231..3793)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (101-476). E-value:
                     2.2e-19. Score: 62.0. Matches aSDomain:
                     nrpspksdomains_MWG59_RS30770_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956907.1"
                     /locus_tag="MWG59_RS30770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_30770"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_248586322.1"
                     /sec_met_domain="PF00561 (E-value: 1.4e-24, bitscore: 86.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTAALYTSIGSLVLSALAAAPAGAWEGRGSAEARGTAIAAARAT
                     AAGIDFVSCPEKEMLPDSLKCGTVKVPLDYAEPDGKQLELTVSRTGATGPADARQGAFV
                     YNPGGPGASSITFPMAGELPEWKEIAEAYDLVGYAPRGVNGSSAPLTCQDPAAYTKGPT
                     DAPTHPTQEYKERRVARAKAYAEGCATHAGETLRHYTSLNNARDLDVLRAALGEPRLTF
                     MGASYGTYFGALYATLFPSHVRRMVFDSVVDPDPEQIWYRSNMGQSLAFEKRWEDFRRW
                     VAKHDDVYHLGDTPEAVQRHYDEVRAELAEKPAGGKVGPGQLHSAFLGAGYYDDYWAMR
                     ATALSEYVRGNPEPLVAQASPRAVAAEDNENAQAVYTAVECNDAPWPEDFEVWDRDHTD
                     LARVAPFETWDNAFANLPCAFWPAPRQRPLEVGTRWGELPPVLILAAERDAATPYKGAL
                     ELRKRLPGSSLVTERDAGTHGIGGAGNTCVDDHLRRYLLTGEVPGRAADCAAHPEPNPV
                     SLD"
     aSDomain        complement(2366..3490)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MWG59_RS30770_Abhydrolase_1.1"
                     /evalue="2.20E-19"
                     /label="MWG59_RS30770_Abhydrolase_1.1"
                     /locus_tag="MWG59_RS30770"
                     /protein_end="476"
                     /protein_start="101"
                     /score="62.0"
                     /tool="antismash"
                     /translation="AFVYNPGGPGASSITFPMAGELPEWKEIAEAYDLVGYAPRGVNGS
                     SAPLTCQDPAAYTKGPTDAPTHPTQEYKERRVARAKAYAEGCATHAGETLRHYTSLNNA
                     RDLDVLRAALGEPRLTFMGASYGTYFGALYATLFPSHVRRMVFDSVVDPDPEQIWYRSN
                     MGQSLAFEKRWEDFRRWVAKHDDVYHLGDTPEAVQRHYDEVRAELAEKPAGGKVGPGQL
                     HSAFLGAGYYDDYWAMRATALSEYVRGNPEPLVAQASPRAVAAEDNENAQAVYTAVECN
                     DAPWPEDFEVWDRDHTDLARVAPFETWDNAFANLPCAFWPAPRQRPLEVGTRWGELPPV
                     LILAAERDAATPYKGALELRKRLPGSSLVTERDAG"
     gene            complement(3943..4995)
                     /locus_tag="MWG59_RS30775"
                     /old_locus_tag="MWG59_30775"
     CDS             complement(3943..4995)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059404.1"
                     /locus_tag="MWG59_RS30775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_30775"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_248586323.1"
                     /transl_table=11
                     /translation="MNVLALLATLAVVVSSVLLIVKAVAERSRRPSPGPGVVLHDLYEV
                     AFLNGGPARVVDTALTALHADGRLAVGGPGIVAVQRAQANDPVERSVFQELAAAPNGAL
                     HVLREAVMRHPAVQEVGDGLAARGLLVPPHRLRPLRQWGLTQGIACMIALPLSLLLTFV
                     QFATDDSLDFSVPFIVKMLPAIVIGSFTGMIVASSSAGRLTASGRHAAQSFRAAYAYVV
                     TPAHLVATLGLRALPDPVFQAQLMAAARLSAPRRRSSARVAGSTATGVTAVGAATVWCA
                     ASGPGGSSCGGSSGGGSGSSCGGGSGCSSGSGCGGGSGSSCGGGSSCGSSSGSSCGGGG
                     GGSSCGGGGS"
     misc_feature    complement(4997..5008)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: strong, score: 21.4"
                     /tool="antismash"
     gene            complement(5001..6329)
                     /locus_tag="MWG59_RS30780"
                     /old_locus_tag="MWG59_30780"
     CDS             complement(5001..6329)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452208.1"
                     /locus_tag="MWG59_RS30780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_30780"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_097931624.1"
                     /sec_met_domain="DUF692 (E-value: 9.3e-91, bitscore: 302.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELGIGIGWRPEIAAEVESLSGIDWVEAVAENLCADHLPDSLVRL
                     RERGVTVVPHGVALGLGGADRPDPVRLASLAARAELLGAPLVTEHIAFVRAGGPLTASP
                     RLEAGHLLPVPRTWDALDVLCENVRIAQDSLPVPLALENIAALITWPDEELTEGQFLAE
                     LVERTGVRLLIDVANLHTNHVNLGQDPAKALDELPVEAIAYVHVAGGVERNGVWHDTHA
                     HPVTAPVLEVLAELRSRVDPPGVLLERDDAFPPGAELAGELDAIRATLRKAAPPADPRP
                     GPAPASDDSAGAGAGTRTAAPTPVPAGSRDRTAVAQTALLSALVAGTPAPAGFDHRRLR
                     VQSRALAAKRADVVAKVAPELPEILGDGYRAAFLAYAGSRPMSGGYRRDALDFAEHVLI
                     AGGPADPVARRRLTYWWQDRSGARPPRRTTRLVRAARAALVRK"
     gene            complement(6452..7198)
                     /locus_tag="MWG59_RS30785"
                     /old_locus_tag="MWG59_30785"
     CDS             complement(6452..7198)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072530.1"
                     /locus_tag="MWG59_RS30785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_30785"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_179890087.1"
                     /transl_table=11
                     /translation="MRRINGTALIIAALVATLGALAFPVWSYADRSGTGEANLNASSVA
                     TQWGPLSATDRDFLVKVRLAGLWELPAGQQAIERAPSEATKAAGDHLVVGHADLDRRAR
                     DVAAKLGVELPNQPTAQQQGWLRELTAASGDAYERKFANILRAAHGKVFGLIGQVRHTT
                     RNTLIRQLASDANQTVLDHITMLEATGFVDFDALAREAASGSTAGPSGPPMPQDGQAPQ
                     VPLPVTPTGDQSFTSRPVPPTMMPMP"
     gene            complement(7339..8067)
                     /locus_tag="MWG59_RS30790"
                     /old_locus_tag="MWG59_30790"
     CDS             complement(7339..8067)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072531.1"
                     /locus_tag="MWG59_RS30790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_30790"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_248586324.1"
                     /transl_table=11
                     /translation="MTSDDTPAPVPESPFHDGPTDRDQAQQFVLPLVLHLEKTDPPART
                     DALRTAARAVLTILSDERSLGDGEWAGAMRDWQDARIRKVVRRARGAEWRKAAALDGIT
                     VTGEDGAEVRVFPPIPLDGWPKELAKLQVSGTDLEDPEPPAAPDPAGPVLWLNPDLDMS
                     AGKTMAQAGHGAQLAWWELSDTERKAWREAGFPLAVATPGAERWRELTVSGLPVVRDAG
                     FTEIAPGSCTVIADHPALRT"
     gene            8139..8813
                     /locus_tag="MWG59_RS30795"
                     /old_locus_tag="MWG59_30795"
     CDS             8139..8813
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378552.1"
                     /locus_tag="MWG59_RS30795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_30795"
                     /product="AIM24 family protein"
                     /protein_id="WP_047179395.1"
                     /transl_table=11
                     /translation="MKSDLFSSDHLAQPATAPGMTLQNTKSIKYAVNGEMHARQGSMIA
                     FRGNLQFERKGQGIGGMLKRAVTGEGLALMAVRGQGEAWFAHEAANCFIVELDQGDSIT
                     INGRNVLCFDPTLSYEIKTVKGAGMVGGGLFNSVFTGYGKLGLMCEGTPIVIPVTAAQP
                     VYVDTDAVVGWSQQLTTSLNRSQSVGSMVRGGSGEAVQLMLQGEGFVIVRPSEARPEKT
                     SN"
     gene            8864..9988
                     /locus_tag="MWG59_RS30800"
                     /old_locus_tag="MWG59_30800"
                     /pseudo=""
     CDS             8864..9988
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018852476.1"
                     /locus_tag="MWG59_RS30800"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_30800"
                     /product="ATP-binding cassette domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLRGVDLTLPARTVVRVDGGNGAGKSTLLRLLAGIDAPAEGRITG
                     RRARTAYVPERFPAALPLTATGYLVHLGSFVRIRSGSRGPARTSAALSSVADAPRLLPP
                     PPCSCTHRTPLPDPT"
     gene            9985..10652
                     /locus_tag="MWG59_RS30805"
                     /old_locus_tag="MWG59_30805"
                     /pseudo=""
     CDS             9985..10652
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452202.1"
                     /locus_tag="MWG59_RS30805"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_30805"
                     /product="ABC transporter"
                     /pseudo=""
                     /transl_table=11
                     /translation="MIALLRYQAALLARSQRWLAPLLLYAAVLAVGVRSGQPVLGSLGV
                     AAAAVLPVTAWAVHLCVAQEPPAARTVASAAAGPGGSTWPRC"
     gene            10667..10870
                     /locus_tag="MWG59_RS30810"
                     /old_locus_tag="MWG59_30810"
     CDS             10667..10870
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378551.1"
                     /locus_tag="MWG59_RS30810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_30810"
                     /product="hypothetical protein"
                     /protein_id="WP_248586325.1"
                     /transl_table=11
                     /translation="MDTDTDDTYCETPGPIAPDPSGGAAEGPPYAECVLCREPTEFPES
                     TKGATLCPVCAWQEAGRTACSG"
ORIGIN
        1 cggcgggaag ctcatgaccg gcgaggtcgc gggcgtccag gtcgacctgg gggccgagtc
       61 gatgctcgcc cggcgccccg aggcggtcgc cctggcccgc gaggtgggcc tcgccgaccg
      121 cctccagccg cccgccaccg ccaccgcctc gctgtggacg cgcgacgcgc tgcgccccat
      181 gccgaagggg cacgtcatgg gcgtgcccgg cgacccggcg gtgctcggcg aggtgctgtc
      241 ccccgagggc ctggcccgga tcgccgagga gcgcgacctc accccgaccc ccgtcggcga
      301 cgacgtcgcg gtcggcgcgt acgtcgccga ccggctcggc cgcgaggtcg tggaccggct
      361 ggtggagccc ctcctcggcg gggtctacgc cggtgacgcc taccggatct cgatgcgcgc
      421 cgccgtcccc cagctcttcg aggcggtgaa ggagggcggg ccgctgctgg agggcgtacg
      481 ccgtatccag gagcgggccg ccgcccggca gcagaccggt cccgtcttcc agggcatcga
      541 gggcggcatc ggcaccctcc cggccgccgt cgccgacgcc gtacgggccg gtggcggcga
      601 gatcctcgac cggacccccg tcctcggcct gacccgtacg gattcgggct gggcggtccg
      661 caccgacacc cggacgatcg ccgccgacgg catcgtcctg gccacccccg cctggtccgc
      721 cggcaccctg ctggccgccg agtccccggc cgcgtccgcc gagctgtccg gcgtcgagta
      781 cgcctcgatg gccctggtca ccctggcctt ccggcgggcc gacatcgagc gccacgaggc
      841 cctgcgcggc cgttccggct tcctcgtacc gccggtcgac gggcacacga tcaaggcgtc
      901 cacgttctcc agcaacaaat ggcagtgggt cgcggacagc gcccccgacc tgttcgtgct
      961 ccgcacctcc gtcggccggt acggcgagga ggaccatctg caccgcgagg acgaggagct
     1021 cgtcgccgtc tccctgcgcg acctcgccgc ggccaccgga ctcaccgccc ggcccgtgga
     1081 caccgaggtc acccgctgga tcggcggcct gccgcagtac ccggtcggcc acctcgcccg
     1141 ggtcgcccgg atccgtgacg aggtcgccaa gctccccgcc ctgcgggtct gcggcgcggt
     1201 ctacgacggg gtcggcatcc ccgcctgcat cgcgagcgcg cgccgggcgg ccgacgagat
     1261 cgtgggggag ctcacggggg agatcgcggg agagatcatc gccacgtcga ccctggttcg
     1321 gggcacgggg agcgaggcgg gacaatagcc gtatgagtgc tcctgagact gtgacatcaa
     1381 gcaagagtcc gaacgccggc aagaaggcca aggacctcaa cgaggtcatc cgctacaccc
     1441 tgtggtcggt cttcaaactg cgcgacgtgc tcccgctgga ccgcgcggga tacgccgacg
     1501 aggtccagga gctgttcgac cagctcgcgg ccaaggacat caccgtccga ggcacctacg
     1561 acgtgtccgg tctgcgggcc gacgccgacc tcatgatctg gtggcacgcc gagacctcgg
     1621 acgagctcca ggaggcgtac aacctcttca ggcgcaccaa gctcggccgc gccctcgacc
     1681 cggtctggtc gaacatggcg ctgcaccggc cggccgagtt caacaagtcc cacgtcccgg
     1741 cgttcctcgc cgacgagacg ccgcgcgact acatcagcgt ctaccccttc gtccggtcct
     1801 acgactggta cctgctgccc gacgaggacc gccgccgtat gctcgcggac cacggcaaga
     1861 tggcccgggg ctacccggac gtccgcgcca acaccgtcgc ctcgttctcg ctgggcgact
     1921 acgagtgggt cctcgccttc gaagcggacg agctgtaccg catcgtcgac ctgatgcgtc
     1981 acctgcgcgc ctccgaggcg cgtctccacg tgcgcgagga ggtcccgttc tacacgggcc
     2041 gcaggaagag cgtcgcagac ctggtggccg ggctcgcata accagcgagt cccgcaccac
     2101 cccaacccgg aagatcagaa gggcgggcac cgggcaccga tcgtgccgtc cgctcctcca
     2161 gcgacaccgg gttcggcttc ggggggnnnn nnnnnncccc cccccgctcc gtaccgcccc
     2221 cgccggggga tcagtccagc gacaccgggt tcggctccgg gtgcgcggcg cagtccgcag
     2281 cccgccccgg cacctcaccg gtcagcagat accggcgcag atgatcgtcg acgcaggtgt
     2341 tcccggcccc gccgatgcca tgcgtcccgg cgtcccgctc ggtcaccagc gacgacccgg
     2401 gcagccgctt ccgcagctcc agcgccccct tgtacggggt cgccgcgtcc cgctccgccg
     2461 ccaggatcag cacgggcggc agctcgcccc accgcgtccc gacctccagc ggccgctgcc
     2521 ggggcgcggg ccagaaggcg cacggcaggt tcgcgaaggc gttgtcccag gtctcgaacg
     2581 gtgcgacgcg cgccaggtcc gtgtggtcgc ggtcccacac ctcgaagtcc tcgggccagg
     2641 gcgcgtcgtt gcactcgacg gccgtgtaca ccgcctgcgc gttctcgttg tcctcggccg
     2701 ccaccgcacg cggggaggcc tgcgcgacca gcggctccgg attgccccgc acgtactccg
     2761 acagcgccgt cgcccgcatc gcccagtagt cgtcgtagta gccggccccc aggaacgccg
     2821 agtgcagctg ccccggcccg accttcccgc ccgccggctt ctccgccagc tcggcgcgca
     2881 cctcgtcgta gtgccgctgc accgcctcgg gtgtgtcccc caggtggtag acgtcgtcgt
     2941 gcttcgccac ccagcggcgg aagtcctccc accgcttctc gaaggccagc gactggccca
     3001 tgttcgagcg gtaccagatc tgctcggggt cggggtcgac caccgagtcg aagaccatgc
     3061 gccgcacgtg ggaggggaac agcgtcgcgt acagcgcgcc gaagtacgtc ccgtaggacg
     3121 cccccatgaa cgtcagacgc ggctcgccga gagcggcccg caggacgtcc aggtcacggg
     3181 cgttgttcag cgacgtgtag tgccgcagcg tctcgcccgc gtgcgtcgcg cagccctccg
     3241 cgtacgcctt cgcacgggcg acgcgccgct ccttgtactc ctgcgtcggg tgggtcggcg
     3301 cgtcggtcgg gcccttcgtg tacgccgccg ggtcctggca ggtcagcggc gcggaggagc
     3361 cgttcacccc gcgcggggcg tagccgacca ggtcgtacgc ctcggcgatc tccttccact
     3421 ccggcagctc cccggccatc gggaaggtga tgctggacgc gccggggccg cccgggttgt
     3481 agacgaacgc gccctgccgg gcgtcggccg ggccggtggc cccggtgcgg ctgacggtca
     3541 gttcgagctg cttcccgtcc ggttcggcgt agtcgagggg caccttcacc gtgccgcact
     3601 tcagggagtc cgggagcatc tccttctccg ggcaggagac gaaatcgatc ccggcggccg
     3661 tggcgcgggc ggcggcgatc gcggtgcccc gggcctcggc ggagccccgg ccctcccagg
     3721 ctcccgcggg agcggcggcg agggcggaca ggaccaggga gccgatcgat gtgtagagcg
     3781 cagcagttct cacgcgcggt ggcctctctg cggatgcgga gaggaagagt cgggcggccg
     3841 gacggatatg tcgagcaccg ccccggaggg tcgccggaat ccatcctgat ggacgctcaa
     3901 gaaccgtacg tacgagactg attcaggtcg cgggagaccg attcaggagc ccccgccccc
     3961 acagctggag ccgccgcccc cgcccccaca gctggagccg ctggagctgc cgcaactgga
     4021 gccgcctccg cagctcgacc cgctgccgcc gccgcacccg gaccccgagg agcacccgga
     4081 gccgcctccg cagctcgatc cggagccgcc cccggacgac ccgccgcaac tggagccacc
     4141 aggaccggac gcggcgcacc agaccgtcgc cgcgccgacg gcggtgaccc ccgtcgccgt
     4201 ggacccggcg acgcgcgcgg aggaccgtcg gcgcggagcg ctcagccgtg ccgcggccat
     4261 caactgggcc tggaacaccg ggtccggcag ggcgcgcagg cccagggtgg cgacgaggtg
     4321 ggcgggggtc accacgtagg cgtaggcggc ccggaacgac tgggcggcgt ggcgccccga
     4381 cgccgtgagg cggcctgcgg aggagctggc gacgatcatc ccggtgaacg agccgatcac
     4441 gatcgcgggc agcatcttca cgatgaacgg gaccgagaag tccagggagt cgtccgtggc
     4501 gaactggacg aacgtcagca gcaggctcag cggcagggcg atcatgcacg cgattccctg
     4561 ggtcaggccc cactgccgca gcgggcggag ccggtggggc ggcaccagca gcccgcgcgc
     4621 cgcgagcccg tcgccgacct cctggaccgc cgggtgccgc atcaccgctt cccgcaggac
     4681 gtgcagggcg ccgttcgggg cggcggccag ctcctggaac accgagcgtt ccaccgggtc
     4741 gttcgcctgt gcccgctgta cggcgacgat gccgggcccg ccgacggcca gccggccgtc
     4801 cgcgtgcagg gcggtcaggg cggtgtccac gaccctggcc ggaccgccgt tcaggaacgc
     4861 gacctcgtac aggtcgtgaa ggacgacgcc ggggcccgga gaggggcggc gggaccgctc
     4921 ggccaccgcc ttgacgatga gcaggaccga tgagacgacg accgcgaggg tcgccagcag
     4981 agcgagcacg ttcacggcgt tcacttcctc acgagggcgg cacgggccgc ccggaccagg
     5041 cgggtggtac gacgcggcgg ccgggcgccc gaccggtcct gccaccagta cgtcagccgg
     5101 cgccgcgcca ccgggtcggc gggaccgccc gcgatgagca cgtgctccgc gaagtccagg
     5161 gcgtcgcgcc ggtacccgcc ggacatcggg cggctcccgg cgtacgcgag gaacgcggcg
     5221 cgatagccgt cgccgaggat ctccggcagt tcgggggcca ccttggcgac gacgtccgcg
     5281 cgcttggcgg ccagcgcccg gctctgcacc cgcagccgcc ggtggtcgaa gcccgcgggg
     5341 gcgggcgttc cggcgaccag ggcggagagc agggcggtct gcgcgacggc ggtacggtcg
     5401 cgggatccgg cgggcacggg ggtgggggcg gccgtacggg tcccggcgcc ggcgcccgcg
     5461 ctgtcgtcgg acgcgggagc cgggcccgga cgcgggtccg ccgggggagc cgccttccgc
     5521 agtgtcgccc ggatcgcgtc cagttcgccc gccagctccg cgcccggcgg gaaggcgtcg
     5581 tcgcgctcca gcagcacgcc cggcgggtcg acccgggagc gcagctcggc cagcacctcc
     5641 agcaccggag cggtcaccgg gtgggcgtgc gtgtcgtgcc agacgccgtt cctctcgacc
     5701 ccgcccgcca catggacgta cgcgatggcc tccaccggca gttcgtccag cgccttcgcc
     5761 gggtcctggc cgaggttgac gtgattggtg tgcaggttgg cgacgtcgat cagcagccgt
     5821 accccggtcc gctcgaccag ctccgccagg aactgcccct cggtcagctc ctcgtccggc
     5881 caggtgatca gcgccgcgat gttctccagg gcgagcggca ccggcagcga gtcctgcgcg
     5941 atccgcacgt tctcgcacag cacgtccagc gcgtcccagg tgcgcggcac cggcagcaga
     6001 tgccccgcct ccagcctcgg cgacgcggtc agcggccccc cggcccgtac gaacgcgatg
     6061 tgctcggtca ccaggggcgc ccccagcagc tcggcgcgcg ccgcgaggga ggcgaggcgc
     6121 acggggtccg ggcggtcggc gccgccgaga ccgagcgcca ccccgtgcgg cacgacggtc
     6181 accccgcgct cccggagccg caccagcgag tccgggaggt ggtcggcgca gaggttctcc
     6241 gcgaccgcct ccacccagtc gatcccgctc agcgactcca cctcggccgc gatctccggc
     6301 cgccagccga taccgatacc cagttccatg acgccccccg tccccctgct cacctcgttc
     6361 tgtccagggg tcatggcccc gtcacgcggc gatgaacccg accaggggga cgttcagagc
     6421 tggatttgag gttcccgtac cgccggtccc gttacggcat cggcatcatc gtgggcggca
     6481 ccgggcgtga ggtgaaggac tggtcaccgg tcggcgtcac cggcagcggg acctgcggcg
     6541 cctggccgtc ctggggcatc ggcgggcccg aggggccggc cgtcgacccg ctcgccgcct
     6601 cgcgcgccag ggcgtcgaag tcgacgaagc cggtggcctc cagcatggtg atgtggtcca
     6661 gcacggtctg gttggcgtcg ctcgcgagct gccggatcag cgtgttccgg gtggtgtggc
     6721 ggacctggcc gatcaggccg aagaccttgc cgtgggcggc ccgcaggata ttggcgaact
     6781 tccgctcgta cgcgtccccg ctcgccgccg tcagctcccg cagccacccc tgctgctggg
     6841 cggtcggctg gttgggcagc tccaccccga gcttcgccgc cacgtcccgg gcccgccggt
     6901 ccaggtccgc gtgcccgacg accaggtggt ccccggccgc cttcgtcgcc tcgctggggg
     6961 cccgttcgat cgcctgctgc ccggcgggca gctcccacag cccggcgagc cgcaccttga
     7021 ccaggaagtc ccggtccgtc gcggagagcg ggccccactg ggtggcgacg ctggaggcgt
     7081 tgaggttcgc ctcgccggtg cccgatcggt ccgcgtagga ccagaccggg aacgcgagcg
     7141 caccgagcgt ggcgaccagc gccgcgatga tgagggccgt tccgttgatg cgtcgcaaca
     7201 aggtgtctcc cgggccgagg cgtgacagcg tcgatcgaac aggcgtcaat caaccgccaa
     7261 tcgactggct gcgcaggcta cttggcggta atgccgatag ggcagtaccg ggagatacgt
     7321 aagaggaacc ggaagtgttc aggtgcgcag cgccgggtgg tcggcgatca ccgtgcagga
     7381 ccccggggcg atctcggtga accccgcgtc gcgcaccacc ggcagcccgc tcaccgtcag
     7441 ctcccgccag cgctccgcac cgggggtggc gacggcgagc gggaacccgg cctcgcgcca
     7501 ggccttgcgc tcggtgtcgg acagctccca ccaggcgagc tgcgccccgt gccccgcctg
     7561 cgccatcgtc ttgcccgccg acatgtccag atccgggttg agccacagca ccgggccggc
     7621 gggatcgggc gcggcgggcg gctcggggtc ctccagatcg gtgcccgaaa cctggagctt
     7681 ggcgagctcc ttgggccagc cgtccagcgg gatcggcggg aacacccgta cctccgcgcc
     7741 gtcctcgccc gtcaccgtga tgccgtccag cgccgcggcc ttgcgccact ccgcgccgcg
     7801 cgcccggcgc acgacctttc ggatccgggc gtcctgccag tcccgcatcg ccccggccca
     7861 ctcgccgtcc ccgagggacc gctcgtcgga gaggatcgtc agcaccgcac gggcggcggt
     7921 ccggagcgcg tcggtgcggg cgggcgggtc cgtcttctcc aggtggagca ccagcggcag
     7981 cacgaactgc tgcgcctggt cgcggtcggt cggaccgtcg tggaacgggc tctcggggac
     8041 gggggcgggg gtgtcatcgc tggtcaccgt ttcagtctgc caggtcgaag ccgtcacctg
     8101 cttggcggaa cgaccgggtc cgggtgagga tgctccgcat gaagagcgat ctcttttcca
     8161 gcgaccacct ggcccagccg gccaccgccc ccggtatgac cctgcagaac accaaatcga
     8221 tcaagtacgc cgtcaacggc gagatgcacg cccggcaggg ctcgatgatc gctttccggg
     8281 gcaacctcca gttcgagcgc aagggccagg gcatcggcgg catgctcaag cgtgcggtca
     8341 ccggcgaggg cctggctctc atggcggtcc ggggccaggg cgaggcgtgg ttcgcgcacg
     8401 aggcggccaa ctgcttcatc gtcgagctgg accagggcga ctccatcacc atcaacggcc
     8461 gcaacgtcct ctgtttcgac cccacgctct cctacgagat caagacggtg aagggcgccg
     8521 ggatggtcgg cggcggcctc ttcaacagcg tcttcacggg ctacggcaag ctcggcctga
     8581 tgtgcgaggg caccccgatc gtgatccccg tgacggccgc ccagccggtg tacgtggaca
     8641 cggacgcggt cgtcggctgg agccagcagc tcacgaccag cctgaaccgc tcgcagagcg
     8701 tcggctcgat ggtgcgcggc ggatccggcg aggccgtcca gctgatgctc cagggcgagg
     8761 gcttcgtcat cgtgcggccc agcgaggcca ggcccgagaa gacctccaac tgaccctgaa
     8821 cctaaggggc gtgggccacc ggtacggcct ccgcggcgcc tgggtgctgc gcggggtcga
     8881 cctcacgctg cccgcgcgga ccgtcgtccg ggtcgatggg ggcaacggcg ccgggaagtc
     8941 gaccctgctg cggctgctcg ccgggatcga cgcccccgcc gaggggcgga tcaccggccg
     9001 tcgggcccgt acggcgtacg tccccgagcg cttcccggcg gcgctgcccc tgaccgccac
     9061 cggctacctc gtccacctag ggtctttcgt tcggatcagg tcgggctcgc ggggtccggc
     9121 acgcacatct gccgcgttgt cgtcggtcgc cgacgctccg cgtctactcc ctcctccgcc
     9181 ttgcagctgc acgcaccgga ccccgctccc tgatccgacc tgatccgaac gaaagaccct
     9241 gagccggatc cacggactgc ggagcgccga ggcgaagcgg cgggcgcggg agtggctgac
     9301 ccggttcggg gcggcggacc acgcccgtac cccgctcgcc gaactctcca agggcaccag
     9361 ccagaaggtc gccgtcgccc aggcgctgct cgccgaaccg gatctgctgg tcctggacga
     9421 ggcctggacc ggcctcgaca ccgccgcccg cgccgaactc gaccgggcgg tggcggaacg
     9481 cgtcgcggcg ggcgccaccg tggtcttcgt cgaccacgac ccgcgacgcc tcgccggggc
     9541 ggcggacctc accctgcgga ccgggggcgg cggggtgcac cgggtgacgg ctccggcgtc
     9601 aacgggaccc gcctccggcc cccacgtcct gatcgaggcc gtgggcccgc cgggcgcacc
     9661 gctgcccgag gacctgccgg gtgacccggc ccgggaccgg cggaccggcg cggtccccgg
     9721 cctcgtacgc ctcaccgtcc ccgccccgta ctccgacgcc gtcctggcgg ccctgctcac
     9781 ggcccgtccg ccctggcaca tccggcaggt cgccccggcg gaagcgcacg ggccggccga
     9841 agcaccggca cacggggcgg ccgaggcacc ggcgtacggg ccgacgccga ccgggccggc
     9901 cggtagcgag gtgtccgcga aggcgcccgc gcaggctccc gtacaggcca ggacccccga
     9961 ggcccccgag gtgtccgaag ctccatgatc gccctcctcc gctaccaggc cgccctgctc
    10021 gcccgttccc agcgctggct ggcgcccctc ctgctctacg cggccgtcct cgccgtcggc
    10081 gtgcggagcg ggcagcccgt gctgggctcg ctcggcgtgg cggccgccgc cgtgctgccc
    10141 gtcaccgcgt gggccgtaca cctctgcgtc gcccaggagc cgcccgccgc ccggacggtc
    10201 gcctcggccg ccgcgggccc cggcgggtcc acctggccgc gctgctgacc gccacgggct
    10261 gtgcggcggc gctcgccgtg gtgaccggcg ccgtcgtgct gctcatcagc agcccggtca
    10321 ccgacgacgg cgcccgcgcc gtctcccggc cgtccgccgc gctcgccgga ctgctcgccg
    10381 tcctgtgctg tgtgctgctg ggcgcggccg tgggggcgct cagcacccgc ccggtgccgc
    10441 tcgaccgggg ctggtccgtc gcggcggccg tgctcggttc ggtcctcgcg ctcgtgacca
    10501 ccggatcccc ggcgcgcctc gcggtgaccg ccctggtcac gggatcgcgg accggggcgg
    10561 tccagccgtc cgtcctgccc ctgctcgggg cggcggccct cgccgcggcg tcggcgttct
    10621 tcgtctgccg gatcgccgcc cggcgcgggt gagtaggctc gtgggcgtgg acacggacac
    10681 ggacgacacc tactgcgaga ccccggggcc gatcgcaccg gacccgtcgg gcggggcggc
    10741 cgaaggccct ccgtacgccg agtgcgtgct gtgccgcgag ccgaccgaat tcccggagtc
    10801 gacgaagggc gccacgctct gcccggtctg cgcctggcag gaggcgggcc gcacggcctg
    10861 ctccggctag gaagaggccc agggggtcgg acggggctac tttcgcagca cgcccgggga
    10921 ccggtcctcc atcaactccg acaccttcac gaagcgatag ccgcgctcgc gcagttccgg
    10981 cacgatccgg cgtacggccg cctcggtggc cggggcggcg ctgcgggtgc agtgcatcac
    11041 cacgagggag cccggccgca ccccgtccag cacctgctcg gccaccgcgt ccgcgtccgt
    11101 ggcgaaggcg tcgccgctga ccacgtccca ctgcacggcc gtcacgttcg ccggggccag
    11161 cgcgcgcagt gccgtgtcgt cgtaacagcc gccggggaag cggaagtacg gcaccacgtt
    11221 tcgcgccccc gccttccgga acgccgagaa ggcacgttcc acctcgcggg ccatcgcggc
    11281 cctgccgacc accggcaggc cgtagcaggg ggagctgaag gcgtggtgg
//
