LOCUS       NZ_CP096281            12853 bp    DNA     linear   CON 08-JUN-2024
DEFINITION  Streptomyces sp. WAC00303 chromosome.
ACCESSION   NZ_CP096281
VERSION     NZ_CP096281.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. WAC00303
  ORGANISM  Streptomyces sp. WAC00303
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8548838)
  AUTHORS   Meyer,K.J., Tan,S. and Nodwell,J.R.
  TITLE     Genome Sequence of WAC00303 Direct Submission
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8548838)
  AUTHORS   Meyer,K.J., Tan,S. and Nodwell,J.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Biochemistry, University of Toronto, 1 Kings
            College Circle 5207, Toronto, ON M5S 1A8, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-MAR-2022
            Assembly Method                   :: Flye v. 2.8.1
            Assembly Name                     :: WAC00303
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58.0x
            Sequencing Technology :: Oxford Nanopore; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023210535.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 05:21:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,823
            CDSs (total)                      :: 7,731
            Genes (coding)                    :: 7,293
            CDSs (with protein)               :: 7,293
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 438
            CDSs (without protein)            :: 438
            Pseudo Genes (ambiguous residues) :: 0 of 438
            Pseudo Genes (frameshifted)       :: 228 of 438
            Pseudo Genes (incomplete)         :: 241 of 438
            Pseudo Genes (internal stop)      :: 26 of 438
            Pseudo Genes (multiple problems)  :: 54 of 438
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:23:54
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6450684
            Orig. end                         :: 6463537
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096281.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Justin Nodwell, University
            of Toronto.
FEATURES             Location/Qualifiers
     region          1..12853
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="21"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12853
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12853
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7853](+)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7853
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     gene            complement(1729..2256)
                     /locus_tag="MWG59_RS29710"
                     /old_locus_tag="MWG59_29710"
     CDS             complement(1729..2256)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MWG59_RS29710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MWG59_29710"
                     /product="hypothetical protein"
                     /protein_id="WP_248586227.1"
                     /transl_table=11
                     /translation="MQRGEVWWVQFDERRLVVLLSGDDASGIRVMQVVAPAGVDISGLG
                     IEVTVGAGEGLPFEGVLRLAFPRPGFTPCTWLTTVSRNDLMERAAVLSSAKLSEIDDAL
                     RRAEQPQEPTGFRPSSAHRHPFDRPPTTAGSGSIPTSHADVRSAKSPMGASTHCVDAGP
                     ADRPRPAVSARK"
     gene            complement(2545..2757)
                     /locus_tag="MWG59_RS29715"
                     /old_locus_tag="MWG59_29715"
     CDS             complement(2545..2757)
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /locus_tag="MWG59_RS29715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_29715"
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="WP_282581355.1"
                     /transl_table=11
                     /translation="MSGPAAAADGADVICTVLPDSEEVRTAVGEVLTAAKPGAVVVETT
                     SHFPQAARELVERAGSPGTPPPPPR"
     misc_feature    2712..2728
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.35"
                     /tool="antismash"
     gene            2904..3131
                     /locus_tag="MWG59_RS29720"
                     /old_locus_tag="MWG59_29720"
     CDS             2904..3131
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MWG59_RS29720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MWG59_29720"
                     /product="hypothetical protein"
                     /protein_id="WP_097932064.1"
                     /transl_table=11
                     /translation="MGATFVDGLRLPVVREGPLRCEGGPLRGRTWWLRDVRAPAPYRTA
                     LAPRGDAPFAVRVHGFLDLARPGTAVRRVP"
     gene            complement(3202..4854)
                     /locus_tag="MWG59_RS29725"
                     /old_locus_tag="MWG59_29725"
     CDS             complement(3202..4854)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007454559.1"
                     /locus_tag="MWG59_RS29725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_29725"
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="WP_248586228.1"
                     /sec_met_domain="Aldedh (E-value: 1.4e-48, bitscore: 164.4,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNPTGNPAPPTDPFLNGPTPPTGHSLLSGHSLLSGHSLLAGREVP
                     GTGDAWYAVAAATGEPFGPPHRDASTEQVAEAARLAAADARAFRALPPERRAAFLDACA
                     EEIEALGDALPALAARETGLPAARLTGERGRTCGQLRLFAELVRSGSALGARIDSGPSG
                     TDVRLRRVPLGPVAVFGASNFPLAFSVAGGDTASALAAGCPVVVKAHPAHPGTGEAVAR
                     AVTAAAARTGMPAGVFSLLVGRGHDVGLALVGDPRITAVGFTGSRAGGLALIAAGRSRP
                     VPVPVHAEMSAVNPVIMLDGALAEPEPAAASYVASLTAGAGQFCTNPGLLLLPAGPAGD
                     AFLSASARALKVAEGQVMLTPDIARAYTEGVRRWENVPGVREVARGAAGTGPYAPAPVV
                     LECDAAAYTAHQELTGEVFGAAGLVVRWSGTDELLGLLEELEGQLTATLHAADADRTTA
                     LELLPVLEERAGRVLWGGWPTGVEVCHAMVHGGPWPATSSPGTTSVGTLAVERWLRPVC
                     YQSFPDALLPPELRDGNPLRVPRQTGSGLTRGA"
     misc_feature    complement(4955..4975)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 21.93"
                     /tool="antismash"
     misc_feature    complement(4977..4987)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: strong, score: 22.4"
                     /tool="antismash"
     gene            5001..6161
                     /locus_tag="MWG59_RS29730"
                     /old_locus_tag="MWG59_29730"
     CDS             5001..6161
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007454561.1"
                     /locus_tag="MWG59_RS29730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_29730"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_097937085.1"
                     /sec_met_domain="DAO (E-value: 1.1e-68, bitscore: 231.2,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVVVVGAGIVGAACAFHAAAAGLDVTVLDRGPVGAGTTSRGEGN
                     ILLSDKEPGPELELARLSRDLWDEAAEELGPETVEFETKGGLVVASTPDALGALHAFAA
                     RQAAAGVRTEAVERVGELEPHVAPGLPGGVHYPQDAQVQPVLAAAGLLRAAVRHGARTH
                     TGEAVAAVTGTDGRITGVRTADGTVLPADAVVNAAGTWGGEVGRRLGAPVEILPRRGFV
                     LVTEPLPPMIRHKVYSADYVANVASSDEGLETSCVVEGTRAGTILIGASRERVGFDTSM
                     NPAVVARLAAQACRLFPFLRGVHLIRAYRGFRPYCPDHLPVIGPDPRVPGVLHACGHEG
                     AGIGLAPGTGALVTAQLLGRPWRDADPADHTGLLPDRFLTTGGVPK"
     gene            6158..6421
                     /locus_tag="MWG59_RS29735"
                     /old_locus_tag="MWG59_29735"
     CDS             6158..6421
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018957038.1"
                     /locus_tag="MWG59_RS29735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_29735"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_007454563.1"
                     /sec_met_domain="Fer2_4 (E-value: 6.4e-24, bitscore: 82.7,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAVTAFTVDGEPLPFVPGQTLAAALVASGRVAWRTTRGGQRPRG
                     IFCGIGVCYDCLVTVDGTGGQRACLVQARAGMAVTTGEGDDE"
     gene            6414..7853
                     /locus_tag="MWG59_RS29740"
                     /old_locus_tag="MWG59_29740"
     CDS             6414..7853
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059558.1"
                     /locus_tag="MWG59_RS29740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_29740"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_248586229.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 3.9e-22, bitscore:
                     77.6, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 8.1e-11, bitscore:
                     41.0, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSESADLVVVGAGPAGMAAATTALDGGLRVVLIDSGAAPGGQFWR
                     HPPLSARAAVPTADLHHGLSTYRALCATLSAHERTGRLTLLLNHHVWTTAREEDGFAVH
                     AMDRGRAPEERAVVLRAPALLVATGAYDRQLPFPGWDLPGVLTAGGLQSLLKGGGVVAG
                     SRVVLGGTGPFLLPVAAALAARGAKVVAVCEAAAPSAWLRHPAALLRNPAKWAEGARYA
                     GTLARHRVPVRTRTAIVGAEGEGRVAVVRTAALDAAGRPLPGTERRIEADTVGVGWGFV
                     PQLDLLLPLGCDLADAGDGTVAAAVDDGQRTTVPGLYTAGETCGVGGAALAVSEGRVAA
                     VSVLTDLSAPARPGTRRPAAERRAVARHRSFARAMAQAHPLPPAWPAWLTDDTPVCRCE
                     EVTAGAVRAARADDAAADHRQVKQLTRAGMGWCQGRMCGPAVHCLVSARDQPYAPAERL
                     IATPVTLGALADSGEPTTDPS"
     gene            7863..8771
                     /locus_tag="MWG59_RS29745"
                     /old_locus_tag="MWG59_29745"
     CDS             7863..8771
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /GO_process="GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007454567.1"
                     /locus_tag="MWG59_RS29745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_29745"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_097937087.1"
                     /transl_table=11
                     /translation="MDRTSLPTRMPWHGVIVATSLPFDQDLAVDHGAYGDNVAWLAEQG
                     LHGVAPNGSLGEYQTLTYEERDRVVETAVANAPEGFTVMPGVGAYGAIEARRHARFAKD
                     AGCQAVMCLPPNSYRADDRAVLEHYAMVASAGLPVTAYNNPIDTKVDLRPELLAKLHAE
                     GFIVGVKEFSGDVRRCYAINELAPGLDLIIGTDDTLLEVGVAGAKGWVAGYPQVFPRAC
                     LDLYNASLAGDLDTALPLYRQLHSVLRWDSRTEFVQAIKLGQDMIGRTGGVCRPPRQTL
                     DPETEAVVRAATQALIDAGVN"
     gene            8773..9774
                     /locus_tag="MWG59_RS29750"
                     /old_locus_tag="MWG59_29750"
     CDS             8773..9774
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378674.1"
                     /locus_tag="MWG59_RS29750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_29750"
                     /product="proline racemase family protein"
                     /protein_id="WP_097937088.1"
                     /transl_table=11
                     /translation="MRSTVCYHAVDSHTEGMPTRVVTGGVGVLPGATMAERRQRFMAQR
                     DGLRTLLMCEPRGHGAMSGAILQPPTRPDADFGVLFIEVSGCLPMCGHGTIGVATVLVE
                     TGMVEAVEPETLIRLDTPAGLVTARVAVRDGRAESVTLENVASYSHALDQVVEVEGFGP
                     VRYDMAYGGNFYAIVRTEDLGIPFDRAEKGRLLEAGLAVMGAINERNPVVHPENPAIDV
                     CHHVYLEAPGSTAEHSRHAMAIHPGWFDRSPCGTGTSARMAQLHARGLLKTGQDFVNES
                     FIGSRFTGRVLAETTVGGRPAVLPSVTGRAWITGTAQYLLDPTDPYPTGFTV"
     gene            9771..10847
                     /locus_tag="MWG59_RS29755"
                     /old_locus_tag="MWG59_29755"
     CDS             9771..10847
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007454570.1"
                     /locus_tag="MWG59_RS29755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_29755"
                     /product="proline racemase family protein"
                     /protein_id="WP_097937089.1"
                     /transl_table=11
                     /translation="MTTDPYTVPIGTAYTGPIDTVDWHTGGEPFRIVPGSPPAVTGHGP
                     TVAERRTAAMADAGVQWTRALLCGEPRGHADMYGAFLVPPDDAGAHLGALFWHKDGFST
                     ACGHGTIALGAWAVATGLVPAPADGTTDVVIDVPSGRVTASVRTAGGRVADVTFVNVPG
                     HVHARGVEVATSLGTVTVDIAYGGAMYAVLPAHRLGLRVRPRDVTAIVAAGREIRDALN
                     AARAAEHPEDDRLSGVYGTVFTEEAGAPVERPDGTWLLHQRNVTVFADGQVDRSPCGSG
                     TAARVALLADAGELRLGDELRHESVVGSVFRARIERSTTGHGRPSVVPAVTGTAYATGT
                     SRFTVDPDDALVPGFVLR"
     gene            10844..11787
                     /locus_tag="MWG59_RS29760"
                     /old_locus_tag="MWG59_29760"
                     /pseudo=""
     CDS             10844..11787
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1158: ornithine cyclodeaminase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072438.1"
                     /locus_tag="MWG59_RS29760"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_29760"
                     /product="ornithine cyclodeaminase family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTVTLDAADVFALGPAGAVAAVREALAGGLDPDGDVPRAVVPLAH
                     GQGLLMPSESGGWFGVKIATVAPGNAARGLRRINATYLLHDSATLTPVALLDGVALTTL
                     RTPAVSVAACLDRLRALADGGGGLCLVVFGAGPQGEGHVAAVRAHVPVADVTVVTRSAG
                     PVPGWADRRLAAAAAGKWRLRCGARTSSSPRRPRADRWSTGGWCGTARWCWRSAPTNRT
                     RGSSTAF"
     gene            complement(11936..12646)
                     /locus_tag="MWG59_RS29765"
                     /old_locus_tag="MWG59_29765"
     CDS             complement(11936..12646)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006128212.1"
                     /locus_tag="MWG59_RS29765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_29765"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_050357013.1"
                     /transl_table=11
                     /translation="MSELKSRKLISVQEHLRDQVANALRAALIAGELQPGVIYSAPTLA
                     AEYGVSATPVREAMLDLAREGLVEAVRNKGFRVTELSERDLDEYTEIRALIEIPTIGRV
                     TRTASREQLEAVRPVAEEIVTAAQGGDLIAYLESDRRFHLDLLALAGNARLVETVADLR
                     KRSRLYGLTDLSQEGSLVGSAREHTALLDIMIAGDAEGAEEHMRRHLAHVRTLWAARKR
                     EAERAAPSRKLGAR"
     misc_feature    12675..12691
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.35"
                     /tool="antismash"
     misc_feature    12679..12689
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 20.42"
                     /tool="antismash"
     misc_feature    12710..12725
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.96"
                     /tool="antismash"
ORIGIN
        1 gcacccgtga cgagaaggat ggcgagtgag agagcggaga gggtgatgat gccgttggag
       61 aaggcgagcc ggtgcccgcg ccgggtgagc ggccgtggca ggaagcggtc ctgtgcgacg
      121 aagctggcca ggtacgggaa cccggtgaag ggagtgttgg cgccggtgta gaggatgagt
      181 gcggttgcga gctggacgaa gaccagaccg atcgtccccg ctgtcccgtt cccgaaggcg
      241 agccgtgcct cctgtgcgat gacggtcggg gttccgtcgg tgtacgggac cgcgtgggtg
      301 aagtgcgcga gggtggagac gccgaggacc agggcggcga gcacgcagct catggtgatg
      361 agggtgtgcc gggcgttgcg gccctgcggt tcgcggaagg cggagatgcc gttcgagatc
      421 gcttcgagcc cagtcagtga cgagccgccg ttggcgaagg aacgcagcac gatgaagagc
      481 gacgcaccgt agagccagcc atcgccggga gttcccagag gcaccacccc cggagcattc
      541 aggtcagcat gcggcagctc gcccatcagt gcgcgcaccg ccgcgacgag gaagaccagg
      601 ctcattgcgg cgatgaagag atacgcgggc agcgcgaaca tccgaccggc ctcgcgaaca
      661 ccgcgcagat tgccgtacgc gagcagcacg atcaataccg cggagaacgg caactggagg
      721 tggtccagcc ccgtccagcc gttgccggcc agatgggcga gggagatcag tgcgttcgtg
      781 ccggccgata cctggacggc gacggtgacg acgtagtcga ccagcagggc cacggcagcg
      841 acctgcgcga cgttgggccc gaaggtctcc cgggccacca cataggagcc accggcccgg
      901 gtgtagatcg tgacgacatc gctgtagcag agcgtcagca gcagcagcac cagcagaatt
      961 gcacccgtca ccggcatgac catcgtgaag gcagctgctc cgaccaccgg cacaaggacc
     1021 cggagcatct gctcggagcc gtaggccgac gaactgatgc agtcggacgc gagcacaccg
     1081 agcgccgtcc ggttgctcag cttctcctcg ctgacacgtt cagtggtgag cggcttgcca
     1141 agaagccgcc gcttgacgcg gtacatcggc cgatcaaggg tcatacgacc atcagaccgg
     1201 gcggctgctc caggaccggt cagacaccac cggcaggcac tcacacatac ccgccgggcc
     1261 cctttctccc ggtggtcccg ccgccaggtc cgtggtcagc cggcccgacc acagcagggc
     1321 catcagccgg ccgcgccgcc ccgggctcgc ggtctcgagc caggcgccgc tcggcgagtg
     1381 ccggctcctc gccgtcgacc acctcgcgcc acgcacgttc cacatcggcg tcgggcacga
     1441 caccgcggtg gcggtaggcg gccaggaacc ggacgttctc cagcaggtcg gctcggcgcc
     1501 cgaccagatc tcgcgctccc agccgtggcg ctcaggaggt gtttgggcca ggccagcgat
     1561 cgggcgacct cggggtcctc caggcgcgag gccggcttga cgtcgacgac gcgcacgcgc
     1621 gccgttcggt ccgccggcat gcgtgtctca tccagaggcg cagcatgtca ggcaccgagt
     1681 cggtctccgc cgcaggtcgt cgactgcatg gctcgccgcg cccgttcgtc acttccgggc
     1741 gctgaccgcg ggtcgcggcc ggtccgcagg gcctgcgtca acgcaatgcg tggaggcacc
     1801 cattggtgac tttgccgacc ggacgtccgc atggcttgtt ggaatcgatc cagaaccggc
     1861 tgtagtgggc ggccggtcga agggatgacg atgggcactg ctcggacgga agccggtcgg
     1921 ctcctgcggt tgttcagcgc gtcggagggc gtcgtcgatc tcgctgagct tcgcggagga
     1981 caggacggcc gcccgctcca tcaggtcgtt ccgggacacc gtggtcagcc acgtgcacgg
     2041 ggtgaagccc ggacgtggga acgcgagccg cagtacgccc tcgaacggca gtccttcacc
     2101 ggcgccgacc gtcacttcga tgcccagacc gctgatgtcg acgcccgccg gagcgacgac
     2161 ctgcatcacc cggatcccgg acgcgtcgtc acccgacagc agtacgacca gcctccgctc
     2221 gtcgaactgg acccaccaga cttcgccacg ttgcacaagt cctccagacc atgacggcag
     2281 acagacgctg cgccaccctg acgcgctgtg gagacgggtt cggccaccgt tgatcatcga
     2341 tctgtgaggg ccgaagatca cgcggtggca cgcaggtcac agcgtagacc ccgctcgccg
     2401 gcgggtcccc tgccgcggac tcatcgagcg tgcgaggtgt gcggcggcgt cacgccgggg
     2461 ccgggtccac gcgttccagg gcggtgaggg accacgaacg ggcgctgctc gccagcagca
     2521 ggacgcgtcg gccgtgcatc tagctcaacg aggtggagga ggcggcgtcc ctggcgatcc
     2581 tgcccgctca acgagttcgc gggcagcctg cgggaagtgg gaggtcgtct ccacgacgac
     2641 cgcgccgggc ttcgcggccg tgagcacctc cccgacggcc gtgcggacct cctcggagtc
     2701 gggcagcacg gtgcagatga catcggcacc gtcggccgcc gccgcggggc cgctcaccgt
     2761 gtgccattcc ggcggcagcg ccgcggggcg cgggacgaga ccacgacgtc gtgccccgcc
     2821 gcgatcagac gctccaccat gggcgccccc atgggtccgg cgccgatgaa tcccacgcgc
     2881 agggaaatgt ccccgtcaag gcggtggggg cgacgttcgt cgacgggctg cggctgccgg
     2941 tcgtgcggga ggggccgctg cgctgcgagg gcgggccgtt gcgcggtcgc acgtggtggc
     3001 tgcgcgatgt gcgggcgccc gccccgtacc gcacggccct cgctccgcgg ggcgacgccc
     3061 ccttcgccgt acgggtgcac ggcttcctgg atctcgcccg cccgggaacc gccgttcggc
     3121 gcgttccgtg acccggaggc gtcggctccg ggtccacggc ggaggggcga ccggcccgtt
     3181 cggccccggt cctgccgggg ttcaggctcc ccgggtgagg ccggaccccg tctgccgggg
     3241 gacccgcagg gggttgccgt cgcggagttc ggggggcagg agggcgtcgg ggaaggactg
     3301 gtaacagacc ggacgcagcc agcgctcgac ggcgagggtg ccgacactcg tcgtaccggg
     3361 cgaactcgtc gcgggccacg ggcccccgtg caccatcgcg tggcagacct cgacgccggt
     3421 cggccagccg ccccacagaa cgcggcccgc ccgctcctcc agtaccggca gcagctccag
     3481 ggccgtcgtc cggtcggcgt ccgccgcgtg cagcgtggcc gtcaactggc cctccagctc
     3541 ctccagcagg ccgaggagtt catccgtacc ggaccagcgc acgacgagcc cggcggcgcc
     3601 gaagacctcg ccggtgagct cctggtgcgc ggtgtacgcg gccgcgtcgc actccaggac
     3661 gacgggggcc ggtgcatacg ggccggtccc ggccgcgccc cgggccacct cgcgtacgcc
     3721 ggggacgttc tcccaccgcc gtacgccctc ggtgtacgca cgggcgatgt ccggggtgag
     3781 catgacctgc ccctccgcga ccttcagggc ccgggccgag gccgagagga aggcgtcgcc
     3841 cgccggtccg gccggcagca gcaggaggcc ggggttcgtg cagaactgcc ccgcgccggc
     3901 cgtcagcgac gccacgtacg aggcggccgc gggttccggt tcggcgagcg ccccgtcgag
     3961 catgatcacg ggattgaccg cggacatctc ggcgtggacc ggcacgggta ccgggcgtga
     4021 ccggccggcc gcgatcagcg ccagcccgcc ggcgcgggag ccggtgaacc ccaccgcggt
     4081 gatccgcgga tcgccgacca gggcgaggcc gacgtcgtgc ccccggccca ccagcaggga
     4141 gaacacgccg gccggcatgc cggtgcgcgc ggccgcggcc gtgacggcgc gggccaccgc
     4201 ctcgccggtg cccgggtgcg cagggtgggc cttgaccacc accgggcagc cggcggccag
     4261 cgcggacgcg gtgtcgccgc ccgcgaccga gaacgccagg gggaagttgc tcgcaccgaa
     4321 cacggccacc gggccgagag gcacgcgccg gagccgtacg tccgtgcccg agggcccgga
     4381 gtcgatccgg gcgccgaggg cgctcccgct cctgacgagc tcggcgaaga ggcgcaactg
     4441 cccgcaggtg cgaccccgtt caccggtcag ccgggccgcc gggagtccgg tctcccgggc
     4501 cgcgagtgcc ggcagggcgt cgccgagcgc ctcgatctcc tccgcgcagg cgtcgaggaa
     4561 ggccgcccgc cgctcgggcg gcagggcgcg gaaggcgcgg gcgtccgccg cggcgagccg
     4621 ggccgcctcg gcgacctgtt ccgtcgaggc gtcccggtgc ggggggccga acggttcgcc
     4681 ggtcgcggcg gcgaccgcgt accaggcgtc cccggtgccg gggacctcgc ggccggccag
     4741 gagcgagtgc ccggacagga gcgagtgccc ggacaggagc gagtgcccgg tcgggggggt
     4801 gggtccgttc aggaacgggt ccgtgggcgg agcggggttt ccggtcgggt tcacagcgat
     4861 gcgtctccct gtgcgcgccg gtgggcggcg cgcggtcgtc gggtgtccgg gtgaaagtca
     4921 ccggggtggt ggcgttgtcc tcgggtcgtc cggatgtata gcctaagcaa tggcatgtga
     4981 cattgcacta gggagcggcc atgaaagtcg tagtcgtcgg agcgggcatc gtgggcgccg
     5041 cgtgcgcgtt ccacgctgcc gcggcggggc tcgacgtcac ggtcctcgac cgtggtcccg
     5101 tcggcgcggg cacgaccagc cggggggagg gcaacatcct cctctccgac aaggagcccg
     5161 gccccgaact ggagctcgcc cggctcagcc gcgatctctg ggacgaggcc gccgaggaac
     5221 tcggaccgga gaccgtcgag ttcgagacca agggcggcct cgtcgtcgcg agcacacccg
     5281 acgcactcgg ggccctgcac gcgttcgccg cgcgccaggc ggccgcaggc gtccgcaccg
     5341 aagccgtcga gcgcgtcggc gaactcgaac cccatgtcgc gcccggcctc cccggcggcg
     5401 tccactaccc gcaggacgcc caggtgcagc ccgtcctcgc ggcggccgga ctgctgcgcg
     5461 ccgcggtccg gcacggggcc cggacgcaca ccggcgaggc cgtggccgcg gtcaccggga
     5521 ccgacggcag gatcaccgga gtgcgcaccg ccgacggcac ggtgctgccc gccgacgccg
     5581 tggtcaacgc cgccggcacc tggggcggcg aggtcggccg ccgactcggc gcccccgtcg
     5641 agatcctgcc ccgccggggc ttcgtcctcg tcaccgagcc gctgccgccg atgatccggc
     5701 acaaggtcta ctccgccgac tacgtggcca atgtcgcctc cagcgacgag ggtctggaga
     5761 cctcgtgcgt cgtcgagggc acccgcgcgg gcacgatcct gatcggcgcc agccgcgaac
     5821 gcgtcggctt cgacacctcg atgaacccgg ccgtggtggc caggctcgcc gcccaggcat
     5881 gccgcctctt cccgttcctg cgcggcgtcc acctgatccg cgcctaccgg ggcttccggc
     5941 cgtactgtcc ggaccatctg ccggtcatcg gcccggaccc ccgcgtcccc ggcgttctcc
     6001 acgcgtgcgg ccacgagggc gccggcatcg gcctcgcccc cgggaccggc gccctggtca
     6061 ccgcccaact cctcggccgc ccgtggcgcg acgccgatcc ggccgaccac accggcctcc
     6121 tgcccgaccg attcctcacc accggaggtg tgccgaagtg accgcagtga ccgcgttcac
     6181 cgtggacggc gaaccgctgc cgttcgtacc gggacagacc ctggccgccg cgctcgtcgc
     6241 ctcgggccgg gtcgcctggc ggaccacccg gggcggacaa cggccccggg ggatcttctg
     6301 cgggatcggc gtctgttacg actgcctggt gaccgtcgac ggcacgggcg ggcagcgcgc
     6361 ctgcctggtg caggcccgcg cgggcatggc cgtcacgacg ggggagggcg acgatgagtg
     6421 agtccgccga cctcgtcgtg gtgggcgcgg gcccggccgg catggccgcc gccaccaccg
     6481 ccctggacgg cgggctgcgg gtcgttctca tcgactcggg cgccgccccg ggcggccagt
     6541 tctggcgcca ccccccgctc tccgcgcgcg cggccgtgcc gaccgccgat ctgcaccacg
     6601 ggctgagcac ctaccgggcg ctctgcgcca ccctctccgc ccacgagcgc accggacggc
     6661 tcacgctgct gctgaaccac catgtgtgga ccacggcccg cgaggaggac ggcttcgccg
     6721 tccacgccat ggaccggggc cgggcgcccg aggagcgggc cgtcgtgctg cgcgcaccgg
     6781 ccctgctggt ggcgaccggc gcctacgacc gccaactccc cttccccggc tgggacctgc
     6841 ccggggtgct caccgccggc gggctccagt cgctgctcaa gggcggggga gtcgtcgcgg
     6901 gcagccgggt ggtcctcggt ggcacgggcc ccttcctgct gccggtcgcc gccgccctcg
     6961 ccgcacgcgg ggcgaaggtc gtcgccgtgt gcgaggcggc tgcgccctcg gcctggctgc
     7021 gccaccccgc ggccctgctg cgcaaccccg ccaagtgggc cgagggcgcc cggtacgcgg
     7081 gcaccctcgc ccggcaccgc gtcccggtcc ggacccgcac cgcgatcgtc ggcgccgaag
     7141 gggaggggcg ggtcgccgtc gtgcgcaccg ccgccctgga cgctgccggg cgcccgctcc
     7201 cgggcacgga gcgccggatc gaggccgaca ccgtgggcgt cggctggggc ttcgtccccc
     7261 agctcgacct gcttctcccg ctcggctgcg atctcgccga cgcgggcgac ggcaccgtgg
     7321 ccgcggccgt cgacgacgga cagcgcacca cggtgcccgg gctgtacacg gcgggcgaga
     7381 cctgcggcgt gggcggggcc gcactcgcgg tgagcgaggg ccgcgtcgcc gccgtctcgg
     7441 tgctcaccga cctctccgcc ccggcccggc ccggtacccg gcgcccggcc gcggagcgcc
     7501 gggcggtggc ccggcaccgc tccttcgccc gggcgatggc acaggcacac cccctgcccc
     7561 cggcctggcc cgcgtggctg accgacgaca caccggtctg ccggtgcgaa gaggtcaccg
     7621 cgggggcggt ccgggccgcc cgcgccgacg acgcggcagc cgaccaccgg caggtcaaac
     7681 aactgaccag agccggcatg ggctggtgcc agggccggat gtgcgggccc gccgtgcact
     7741 gcctcgtctc cgcccgcgac cagccgtacg cccccgccga gcggctgatc gcgacccccg
     7801 tcaccctcgg tgcgctcgcc gattccggcg agcccaccac cgatcccagc tgaggagttc
     7861 ccatggaccg cacctccctt cccacccgca tgccctggca cggcgtcatc gtcgcgacga
     7921 gcctcccctt cgaccaggac ctcgccgtcg accacggcgc ctacggtgac aacgtcgcct
     7981 ggctcgccga gcagggcctg cacggcgtcg ccccgaacgg ctcgctgggc gagtaccaga
     8041 ccctgacgta cgaggaacgc gaccgcgtcg tggagaccgc cgtcgcgaac gcccccgagg
     8101 gcttcaccgt gatgccgggt gtcggagcgt acggcgcgat cgaggcgagg cggcacgcgc
     8161 ggttcgccaa ggacgccggc tgccaggccg tcatgtgcct gccgcccaac tcctaccggg
     8221 ccgacgaccg tgccgtcctg gagcactacg cgatggtggc ctccgccgga cttcccgtca
     8281 ccgcctacaa caaccccatc gacaccaagg tcgacctgcg cccggagctg ctggccaagc
     8341 tgcacgccga gggattcatc gtgggcgtga aggagttctc cggtgacgtc cgccggtgct
     8401 acgccatcaa cgaactcgcc cccggactcg acctgatcat cggcaccgac gacaccctcc
     8461 tggaagtcgg cgtcgccggc gccaagggct gggtcgccgg ctacccccag gtcttcccgc
     8521 gcgcctgcct cgacctctac aacgcctcgc tggccggcga tctcgacacc gcgctcccgc
     8581 tctaccggca gctccactcc gtactgcgat gggacagcag gaccgagttc gtccaggcca
     8641 tcaagctcgg ccaggacatg atcggccgca ccggcggcgt ctgccgtccg ccccggcaga
     8701 ccctggaccc ggagaccgag gccgtcgtcc gcgccgccac ccaggcactc atcgacgccg
     8761 gggtgaactg acgtgcgctc cacggtctgt taccacgcgg tcgactcgca caccgagggc
     8821 atgccgaccc gggtcgtcac cggcggggtg ggcgtcctgc cgggcgccac gatggccgag
     8881 cggcggcagc ggttcatggc gcagcgcgac gggctgcgca ccctgctcat gtgcgagccg
     8941 cgcgggcacg gggccatgtc cggagccatc ctccagcctc cgacccggcc cgacgccgac
     9001 ttcggcgtgc tgttcatcga ggtctccggc tgcctgccca tgtgcggcca cggcaccatc
     9061 ggcgtggcga cggtcctcgt ggagacgggc atggtcgaag cggtcgaacc ggagacgctc
     9121 atccgcctcg acacccccgc cggcctcgtc acggcacggg tcgccgtgcg cgacggccgc
     9181 gcggagtccg tcaccctgga gaacgtcgcc tcctacagcc acgcgctgga ccaggtcgtc
     9241 gaggtcgagg ggttcggccc ggtccgctac gacatggcgt acggcggcaa cttctacgcc
     9301 atcgtgcgca ccgaggacct cggcatcccc ttcgaccggg cggagaaggg gcggctgctg
     9361 gaggcgggcc tggccgtcat gggggcgatc aacgagcgga acccggtggt gcatccggag
     9421 aaccccgcga tcgacgtctg ccaccacgtc tatctggagg cgcccggctc gaccgccgag
     9481 cactcgcggc acgccatggc catccatccc ggctggttcg accgctcgcc ctgcgggacg
     9541 ggaaccagtg ctcggatggc ccaactgcac gcgcgggggc tgctgaagac cgggcaggac
     9601 tttgtcaacg agtccttcat cggctcccgc ttcacgggcc gggtcctggc ggagaccacg
     9661 gtcggcggca ggcccgccgt actgccctcc gtcacgggcc gcgcctggat caccgggacc
     9721 gcgcagtacc tgctcgaccc caccgacccc tatccgacgg gattcacggt atgacgaccg
     9781 atccgtacac cgtgccgatc gggacggcgt acaccgggcc gatcgacacg gtcgactggc
     9841 acaccggggg cgagccgttc cggatcgtgc ccgggtcgcc gccggccgtg accgggcacg
     9901 gtcccaccgt cgccgagcgc cgcacggccg ccatggccga cgccggggtg cagtggaccc
     9961 gggccctgct gtgcggcgaa ccgcgcggcc acgccgacat gtacggggcc ttcctggtgc
    10021 cgccggacga cgccggagcc cacctcgggg ccctcttctg gcacaaggac ggcttctcca
    10081 cggcctgcgg ccacgggacg atcgccctcg gcgcctgggc ggtcgccacc ggcctggtcc
    10141 cggccccggc cgacggcacg acggacgtcg tcatcgacgt cccctccggc cgggtcacgg
    10201 cgagcgtgcg cacggccggc ggccgcgtcg cggacgtgac gttcgtcaac gtccccggcc
    10261 atgtccacgc gcgcggtgtc gaggtggcca cctcgctggg aaccgtcacc gtcgacatcg
    10321 cctacggcgg cgccatgtac gccgtgctcc cggcacaccg gctcggcctg cgggtccggc
    10381 cgcgtgacgt caccgcgatc gtcgcggccg gccgcgagat ccgcgacgcg ctgaacgccg
    10441 cccgcgccgc cgaacacccc gaggacgacc ggctgtccgg cgtctacggg acggtgttca
    10501 ccgaggaggc aggcgccccc gtcgaacggc ccgacggcac ctggctgctg caccagcgca
    10561 acgtcacggt gttcgcggac ggccaggtcg accgctcccc gtgcggctcg ggtaccgcgg
    10621 cccgggtggc gctcctcgcc gacgccgggg aactgcgcct cggcgacgag ttgaggcacg
    10681 agtcggtggt gggctccgtc ttccgcgccc ggatcgagcg gtccaccacg ggccacgggc
    10741 gcccctcggt cgtcccggcc gtgacgggca ccgcgtacgc cacgggtacc agccgcttca
    10801 ccgtcgatcc cgacgacgcc ctcgtacccg ggttcgtgct gcgatgacgg tgacgctcga
    10861 tgccgcggac gtgttcgcgc tcggtccggc cggggccgtc gccgcggtcc gggaggcgct
    10921 ggccgggggg ctcgacccgg acggcgacgt accgagggcg gtcgtgccgc tggcccatgg
    10981 tcaggggctg ctcatgccct ccgagtccgg cggctggttc ggggtgaaga tcgccaccgt
    11041 cgcgcccggc aacgccgcgc gcgggctgcg gcggatcaac gccacgtacc tgctgcacga
    11101 cagcgcgacc ctgacgccgg tggccctgct ggacggggtg gcgctgacga ccctgcgcac
    11161 ccccgccgtc tcggtcgcgg cctgcctgga ccggctgcgc gcgctcgccg acgggggcgg
    11221 ggggctgtgc ctggtggtct tcggcgcggg tccccagggg gaggggcacg tcgcggccgt
    11281 acgggctcat gtaccggtgg ccgacgtcac ggtcgtcacc cggagcgcag ggcccgtgcc
    11341 cggctgggcg gaccgccgcc tggcggcggc tgcagcgggg aagtggcggc tgcggtgcgg
    11401 cgcgcggacg tcgtcgtcac cgcgacgtcc gcgcgccgac cggtggtcga cgggcggctg
    11461 gtgcgggacg gcgcggtggt gctggcggtc ggctcccacg aaccggacgc gcgggagctc
    11521 gacagcgttc tgatggggcg tgccaccgtc gtggtggaga gccgtgcgac cgccctccgc
    11581 gaggcgggcg acgtcgtcat ggcgatccac gagggctcgc tcgacccgga cgacctcgtc
    11641 accctggcgg atccgaccgg ttcgcggggg gacatcccgg ccgaccgccc gctggtcgtg
    11701 aagacgtgcg ggatgggctg gcaggacctg gtggtcgccg tcgcggccca ccggcggaag
    11761 gaggggacgc cggcggagcg gagctgaccg tccgccgacg cgggccccga caccgcgccc
    11821 gccgggcggt ccgccacgtc aacacgcgcc gccccgccct gacttcgggg cgaggggcgc
    11881 agagtccgcg cggccccgtc acgtcaacgc gcgccgcccc aaacgtactt cggcgtcagc
    11941 gcgcgccgag cttgcgggac ggggcggcgc gttcggcttc gcgcttacgg gccgcccaca
    12001 gggtccgcac atgcgcgaga tgccggcgca tgtgctcctc ggccccctcg gcgtcccccg
    12061 cgatcatgat gtccaggagc gcggtgtgct cgcgcgccga acccaccagc gacccctcct
    12121 gggacaggtc ggtgagcccg tagagccggg agcgcttgcg caggtcggcc acggtctcca
    12181 cgaggcgggc gttcccggcg agggcgagca ggtcgaggtg gaagcggcgg tccgactcca
    12241 ggtaggcgat caggtcgccg ccctgcgcgg cggtaacgat ctcctcggcc accggacgca
    12301 ccgcctccaa ctgctcgcgg gaggcggtgc gggtcacgcg gccgatggtg gggatctcga
    12361 tgagggcgcg gatctcggtg tactcgtcca ggtcgcgctc ggagagttcg gtgacgcgga
    12421 accccttgtt gcgcacggcc tcgaccaggc cctcgcgggc cagatccagc atggcctcgc
    12481 gcacgggagt ggcggagacg ccgtactcgg cggcgagcgt gggcgccgag tagatcacgc
    12541 cgggctggag ttcccccgcg atcagcgcgg cacgcagggc gttggccacc tggtcgcgca
    12601 gatgctcctg caccgagatg agtttgcggg acttcagctc actcatgcat tccccttcaa
    12661 tgatgtggca ccgcactata caatgtcacg ttgcgcggtg cctcatcgct cgaaggtctg
    12721 tgctgtcagc tgtccaggag ccgggccatc caaccctcga caccgtcgac cgtacggggc
    12781 agcgcgccgg agagcagctt cgccccgtcc gccgtgatca ccaggtcgtc ctcgatgcgc
    12841 acgcccatgc cgc
//
