LOCUS       NZ_CP096281            10423 bp    DNA     linear   CON 08-JUN-2024
DEFINITION  Streptomyces sp. WAC00303 chromosome.
ACCESSION   NZ_CP096281
VERSION     NZ_CP096281.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. WAC00303
  ORGANISM  Streptomyces sp. WAC00303
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8548838)
  AUTHORS   Meyer,K.J., Tan,S. and Nodwell,J.R.
  TITLE     Genome Sequence of WAC00303 Direct Submission
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8548838)
  AUTHORS   Meyer,K.J., Tan,S. and Nodwell,J.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Biochemistry, University of Toronto, 1 Kings
            College Circle 5207, Toronto, ON M5S 1A8, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-MAR-2022
            Assembly Method                   :: Flye v. 2.8.1
            Assembly Name                     :: WAC00303
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58.0x
            Sequencing Technology :: Oxford Nanopore; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023210535.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 05:21:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,823
            CDSs (total)                      :: 7,731
            Genes (coding)                    :: 7,293
            CDSs (with protein)               :: 7,293
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 438
            CDSs (without protein)            :: 438
            Pseudo Genes (ambiguous residues) :: 0 of 438
            Pseudo Genes (frameshifted)       :: 228 of 438
            Pseudo Genes (incomplete)         :: 241 of 438
            Pseudo Genes (internal stop)      :: 26 of 438
            Pseudo Genes (multiple problems)  :: 54 of 438
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:23:54
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5464549
            Orig. end                         :: 5474972
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096281.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Justin Nodwell, University
            of Toronto.
FEATURES             Location/Qualifiers
     region          1..10423
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="16"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10423
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10423
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5423](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5423)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            1075..2634
                     /locus_tag="MWG59_RS25400"
                     /old_locus_tag="MWG59_25400"
     CDS             1075..2634
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455545.1"
                     /locus_tag="MWG59_RS25400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_25400"
                     /product="glycosyltransferase"
                     /protein_id="WP_248585936.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 2.1e-27,
                     bitscore: 94.8, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGLQLSVVVPCFDEAEVIEAFHTALVGVLDPLGDSFEICYVDDG
                     SRDRTRLLLSALAARDPRAHYTAFSRNFGKEAAMLAGLRMSRGAAVVIMDADLQHPPEL
                     IPRMLELHRHGYDQVIPRRDRAGEGLLRSTLSHTYYALVRRCMDVELIDGSGDFRLLSR
                     RAVESVLSLPESNRFSKGIFSWIGFDTVTFRYRNSERVAGRSKWGSKRLLNYGIDGLLS
                     FNNRPLRLAIYTGFWVFVSALVYALWTVVGVVLHGADTPGYATLLTAVVALSGIQLVTL
                     GVIGEYVGRIYHEAKHRPPYVVRETDATCGALPDSPPPRPPLTGGPGEPAAPALATTAA
                     PALATTAAPAPATASTAPAPATGAPGRSRTVRQFGSFVLIGCVNTAVYLGIYASLTRWI
                     PYLTAHVIGYAVSIVCSFLLNSYVTCRTRPTWHGFVRYPLSSLVNLVASGALLYGAVSG
                     LGMDKNLAALAAGVLVTPVSFLLARWAITSGRARTDAVPQAEPVPGRPAPAPLPTRSSQ
                     DR"
     gene            2644..4152
                     /locus_tag="MWG59_RS25405"
                     /old_locus_tag="MWG59_25405"
     CDS             2644..4152
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455543.1"
                     /locus_tag="MWG59_RS25405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_25405"
                     /product="DUF6056 family protein"
                     /protein_id="WP_248585937.1"
                     /transl_table=11
                     /translation="MSDDVPMTAAPEGLRGEERADPAGRPADRPWTALWVSALALPPLA
                     LLAAASWFGRWVRPGADDWCFLPAVRDGGVTGLVGKFYLDDNGRVANALLVGAYAKFQV
                     AGHQWYPLASGVLVLAVLWTVAVLALRRARLRTPRGLPLLLAALTTALFLFVTPNTYKT
                     YLWPAASVSHTLPPVLACAALIPFLLARSRRGRAVALAVAALMGAFLATLSEETAIVVV
                     VVLLTALLLSGRVVAGPDRGFVRRWCAAGIAGTAAGAVVLITSPGSMRRRERFGAETTS
                     LLAPDSLTASLRAFAEIAVTVATTWQYAGAVAAGVLLGLLCRRADGTPPRPPAHWPLLT
                     AAGVLALLVSGYLCTVIAHPVFGDRVSDPSANRLWNDYLLLYVILLVGAGALLGLGVRR
                     LTRRTAPAKAVCAALCVLVCVGPAVSLTDLDTAMRARAEKWDAQDRRLREGAEAGRRVM
                     PYERLVISDMLEPFSAGGRRYWPGGCVADLYGLDRVTDGARSAP"
     gene            complement(4160..4984)
                     /locus_tag="MWG59_RS25410"
                     /old_locus_tag="MWG59_25410"
     CDS             complement(4160..4984)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012379204.1"
                     /locus_tag="MWG59_RS25410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_25410"
                     /product="tyrosinase family protein"
                     /protein_id="WP_248585938.1"
                     /transl_table=11
                     /translation="MTVRKNQANLTGPEKKAFVDAVLEVKRIGKYDEFVLAHRTRFSID
                     MNTGVYVGHFGPSFLPWHRKFLIDFENELQKIDPSVSIPYWDWTADNTVGSSLWAPDFL
                     GGTGRPLDDQVMDGPFAHSGGNWTINVSVDSRAFLARQLAYRVPTLPPRSDVDAALAMP
                     TYDSPPFRDGSAGFRSTLEGSAGYMSMHNRVHTWVWGHMETSVSPNDPVFWLHHAFVDK
                     LWADWHALHPTSAEYLPAGGTTDVIDLNDTMPPWPNTTPQSMLDHTQFYTYA"
     gene            complement(5001..5423)
                     /locus_tag="MWG59_RS25415"
                     /old_locus_tag="MWG59_25415"
     CDS             complement(5001..5423)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966560.1"
                     /locus_tag="MWG59_RS25415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_25415"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_097931609.1"
                     /sec_met_domain="melC (E-value: 3.2e-39, bitscore: 133.1,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPRPTRRQALGAVIGVAASGALVGMAAASTETAAAEAESSGIAPG
                     GTGAPGSFDEVFEGRRIQGTPAEARTDAHAHAGHAGHAGYRVLIDGRELPVMQHGKSGW
                     SSTINHYERFATPLEAARTAVISLKGAVVVPFDPTA"
     misc_feature    5571..5585
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 25.09"
                     /tool="antismash"
     misc_feature    complement(5987..6000)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.25"
                     /tool="antismash"
     gene            5994..6662
                     /locus_tag="MWG59_RS25420"
                     /old_locus_tag="MWG59_25420"
     CDS             5994..6662
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455530.1"
                     /locus_tag="MWG59_RS25420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_25420"
                     /product="DUF4232 domain-containing protein"
                     /protein_id="WP_097931607.1"
                     /transl_table=11
                     /translation="MFGKRRKAFLVSAALVGGALLMTACQDTEDGAAPSASATDVPAAD
                     AAGSDASKGTDEGAEQGAGQEPDAKDGGSAEGSGADAGDGTKVPVQQACGANDISWSTR
                     SESQAGGYILIMAKAKQGITCILPAALPTVAFGSDGTEAGPAEQSVGDAVTLSGATTAY
                     AGVNPKTTDTDGGKELDSIIVGVGNEDPDPVSLEVGTITVDKPVVTNWHTAPADAVPAA
                     "
     gene            complement(6718..7737)
                     /locus_tag="MWG59_RS25425"
                     /old_locus_tag="MWG59_25425"
     CDS             complement(6718..7737)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956218.1"
                     /locus_tag="MWG59_RS25425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_25425"
                     /product="hypothetical protein"
                     /protein_id="WP_248585939.1"
                     /transl_table=11
                     /translation="MENTIPRGNWLDDDTFRELVREVTPLRFGSWSLSEFEQATSELGW
                     ELREPKEVVGRVWRRFAPRKGPWGGYGTVIADASEPDQVRKLNVRVVDLPAEDTATAAG
                     LVRAAWWVMEEELGPPTMWGGDSGPWMLWRRPGVCLLVHTHDEGEVSLELLPPDADTDA
                     AGGGYSRGRWRAAELAGLPSAPAAPPAPGTTWEEVEKRLSEALRSLDHDTPFFPGRFIL
                     HLGSADDPQRFVQCWSQDLGLVIEATGYLHRPDAADPARLTRNGWVPSPSVWQRRFPDA
                     MDDPAHAATAARMLVEELRHLGVGLADLSYDGTMTGRGRGFHLELPDLGIPRVHQPPV"
     gene            7841..8293
                     /locus_tag="MWG59_RS25430"
                     /old_locus_tag="MWG59_25430"
     CDS             7841..8293
                     /EC_number="3.1.2.-"
                     /GO_function="GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1144: thioesterase superfamily protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956219.1"
                     /locus_tag="MWG59_RS25430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_25430"
                     /product="PaaI family thioesterase"
                     /protein_id="WP_248585940.1"
                     /transl_table=11
                     /translation="MEAHASELVFGQKVLEAQPFSVLLGARLTAFGDGAAVLELDVRDE
                     LRQQNGFVHGGVLSYAADNALTFAAGAAVGPSVLTAGFTIDYLRPARGEVLRARAQVVR
                     PGRTRVVCRCDLLMVDAAGVETLCAVAQGTIAVAGDSGSRPPHSPL"
     misc_feature    complement(7858..7871)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.45"
                     /tool="antismash"
     gene            complement(8346..9122)
                     /locus_tag="MWG59_RS25435"
                     /old_locus_tag="MWG59_25435"
     CDS             complement(8346..9122)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950999.1"
                     /locus_tag="MWG59_RS25435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_25435"
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="WP_097931601.1"
                     /transl_table=11
                     /translation="MRSRPVGFLLSALAGAALAFGGTAAASATPPSAPGSAPTGNVGTA
                     DACYTWGGNLQEGSSGEAVRQLQIRVAGYPGTGNQIAIDGVFGPATKAAVQRFQAAYGL
                     AADGIAGAQTFSKIYALQDDDCTPVNFTYAELNRCNSDWSGGKVSAATARANALVTMWK
                     LQAMRHAMGDRPITVNGGFRSVSCNSAVGGAANSRHMYGHAADLGAGSQGFCGLAQAAR
                     NHGFTEILGPGYPGHNDHTHVAGGSGRFWSAPSCGI"
     gene            9254..9784
                     /locus_tag="MWG59_RS25440"
                     /old_locus_tag="MWG59_25440"
     CDS             9254..9784
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015609230.1"
                     /locus_tag="MWG59_RS25440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_25440"
                     /product="MepB family protein"
                     /protein_id="WP_097931599.1"
                     /transl_table=11
                     /translation="MAIDRLWAGLHGDLRAAKSSVYDPSGFTCSQPVPEPESADYAAHG
                     FTLDGRVVAFRVARTTPTKAGQFVTVWQRSAEGPIRPFDTDDGVDLFVIGSRDNGHFGQ
                     FVFPREVLCERGIVSRNGVGGKRGFRVYPPGAITVGRQARGTQAWQVKYFLDLGEDGHG
                     PVDLVRARALHHP"
     misc_feature    complement(9846..9861)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.26"
                     /tool="antismash"
ORIGIN
        1 ccagcacctc cttgacctgc ccgtccagct ccggcagtgg tgtcggcggc tccggcgcac
       61 catctgcccc cgcgccccgg ccccagccgg tggccgccgg ggtggtgcgg gtcgtcgtgc
      121 gccgtttgcg ccgggcgtac gcgtaggcgc cgaccgcgcc cacgcccacc acgagggccg
      181 ccggcaggat gaagtcgcgg gcgccggtgg agccggagtc gcccgttccg gggtcggcgg
      241 gccccggggt gatggcgggg gcggtcaccg ggcgtccggc cagtgcggag ccgtagccgt
      301 cggccgcgcc gatcgcggcc cccgcccagt cgttctcccg cagcgcgggt tcgatcgcgg
      361 tgcccgccac gtcccgcagc tgctcgtcgg tgagtgggga gccggcgtcg acggagtagg
      421 cgtactgccg gtcgtgggtg gcgacggaga gcagcacgtc gtcctggccc agaccgttgc
      481 ggtcggcggt ctcgtcgctc caggtctgtc cggaccggcc ggagaaatcg cgtacgtaca
      541 cgacgaagag ctggatgcgc tcgtcgtcgt acaaccggtc gagcgcgtcc tccacctgcc
      601 ccgtccggtc gcccagggct cccacccggt cggtgatctg cccgtcccgg gacagctcga
      661 cggggtcgtc ggcgcgggcg gtcgcggctg cggggagcaa cagccagcac acggcggaca
      721 gcacggcgag caggccggtg agcgtaccgg cgagaggccc ggtggaccgg ccgggaagcc
      781 gggccctgcc gggtgcggtg ctccgggtcc gtggcagtcg aggagatatc acctttgcga
      841 ggctatgtgt gcttttttgc ccccgcgacc cggcgttctg ccgacccgcg tccgctgttc
      901 gcatgaacgg gtgcgggaaa gaggcgtccc cgcgcgtgta cggagagacg gaggccgccg
      961 cccgtcgtca gatggggcgg acgtggcgtg ttccggggcc cggtgccgga gggcgacggt
     1021 gccgcgccgg tgaggcccgg cacccacgag gggcccgaag tccggaggtg gcgtgtgact
     1081 ggccttcagc tctcggtcgt cgtgccgtgt ttcgacgagg ccgaggtgat cgaggccttc
     1141 cacaccgccc tggtcggcgt cctggacccc ctcggggaca gcttcgagat ctgttacgtg
     1201 gacgacggca gccgggaccg cacccgactg ctgctgagcg ccctcgccgc gcgcgacccg
     1261 cgggcccact acaccgcctt cagccgcaac ttcggcaagg aggccgccat gctcgccggc
     1321 ctccgcatgt cgcggggtgc ggccgtggtg atcatggacg ccgacctcca gcacccgccc
     1381 gagctgatcc cccgcatgct ggaactgcac cggcacggct acgaccaggt catcccgcgc
     1441 cgcgaccgcg cgggcgaggg cctgctccgc agcaccctca gccacaccta ctacgcgctc
     1501 gtccgccgct gcatggacgt ggagctcatc gacggctcgg gggacttccg gctgctgtcg
     1561 agacgggccg tggagagcgt cctgtccctc cccgagagca accgcttctc caaggggatc
     1621 ttctcctgga tcggcttcga cacggtcacc ttccgctacc gcaacagcga gcgggtggcg
     1681 ggccggtcga aatggggaag caaacggctg ctgaactacg gcatcgacgg actgctctcc
     1741 ttcaacaacc gcccgctccg cctcgcgatc tacaccgggt tctgggtctt cgtctcggcc
     1801 ctggtctacg ccctgtggac ggtcgtgggc gtcgtcctgc acggcgcgga caccccgggt
     1861 tacgccaccc tcctcaccgc cgtcgtcgcc ctcagcggca tccagttggt gaccctcggg
     1921 gtcatcggcg aatacgtggg ccgcatctac cacgaggcga aacaccgccc gccctacgtc
     1981 gtgcgggaga ccgacgcgac ctgtggggcg ctgccggaca gcccgccgcc gcgaccgcct
     2041 ctcaccggag gcccgggcga acccgccgcc ccggccctcg cgaccaccgc cgccccggcc
     2101 ctcgcgacca ccgccgcccc ggccccggcg accgcctcca ccgccccggc cccagcgacc
     2161 ggcgcccccg gccggtcccg tacggtccgc cagttcggca gcttcgtcct catcggctgc
     2221 gtgaacaccg ccgtctacct cgggatctac gcctcgctga cccgctggat cccctacctg
     2281 accgcccacg tcatcggcta cgccgtcagc atcgtgtgct cgttcctgct caactcctac
     2341 gtcacctgcc ggacgaggcc gacgtggcac gggttcgtcc gctatcccct ctccagcctg
     2401 gtcaacctgg tggcgtccgg agcgctgctc tacggggcgg tgagcggtct cgggatggac
     2461 aagaacctcg ccgcgctggc ggcgggcgtt ctcgtcaccc cggtctcgtt cctgctggcc
     2521 cggtgggcga tcacctcggg ccgggccagg accgacgccg taccgcaggc cgaaccggtg
     2581 cccggccgtc cggcgcccgc accgctgccc acccgctcgt cccaggaccg gtgaggggcc
     2641 gcgatgtccg acgacgttcc catgaccgcc gccccggagg gactgcgggg cgaggagagg
     2701 gcagacccgg ccgggcgtcc cgcggaccgc ccctggaccg ccctctgggt gagcgccctc
     2761 gcgctcccgc ccctcgccct gctcgccgcc gcctcctggt tcgggcggtg ggtgcggccc
     2821 ggcgcggacg actggtgctt cctgcccgcc gtacgggacg gcggcgtcac cggcctcgtc
     2881 gggaagttct acctcgacga caacgggcgg gtcgccaacg cgctcctggt cggcgcgtac
     2941 gcgaagttcc aggtcgcggg acaccagtgg taccccctgg ccagcggggt cctggtgctc
     3001 gcggtcctct ggacggtggc cgtcctggca ctgcgccggg cccggctgcg caccccgcgc
     3061 gggctgccgc tcctgctcgc ggccctgacc accgcgctct tcctgttcgt caccccgaac
     3121 acgtacaaga cgtatctctg gcccgccgcg tccgtctcgc acacgctgcc gcccgtcctg
     3181 gcctgcgccg cgctgatccc gttcctgctc gcccgctccc gccgggggcg ggccgtcgcg
     3241 ctcgccgtcg cggcgctcat gggagccttc ctcgccacgc tgtccgagga gacggcgatc
     3301 gtcgtcgtgg tggtgctcct gaccgcgctc ctgctgtccg gccgggtcgt cgccggcccc
     3361 gaccgggggt tcgtccggcg ctggtgcgcg gcgggaatcg ccgggacggc ggccggggcc
     3421 gtcgtcctca tcacctcacc cggctcgatg cggcggaggg agcggttcgg cgcggagacc
     3481 acctccctgc tcgcccccga ctcactcacc gcgtcgctgc gcgcgttcgc cgagatcgcc
     3541 gtcaccgtgg ccacgacctg gcagtacgcc ggcgcggtcg ccgcaggcgt actgctgggg
     3601 ctgctgtgcc gacgggcgga cgggacaccg ccccgtccgc ccgcgcactg gcccctgctc
     3661 accgccgccg gggtgctcgc tctcctcgtc tccggctacc tctgcacggt catcgcccat
     3721 ccggtgttcg gcgaccgggt gagcgacccg agcgccaacc ggctgtggaa cgactacctc
     3781 ctgctgtacg tgatcctgct cgtcggcgcg ggcgccctgc tcggcctggg cgtgcgccgc
     3841 ctcacccgcc gcaccgcccc ggcgaaggcg gtgtgcgccg cgctctgcgt gctggtctgc
     3901 gtcggcccgg ccgtgtcgct caccgacctg gacaccgcga tgcgggcccg ggccgagaag
     3961 tgggacgccc aggaccgccg gctgcgcgaa ggggcggagg ccgggagacg ggtcatgccg
     4021 tacgagcgtc tggtgatcag cgacatgctg gagccgttca gcgcaggcgg gcgccggtac
     4081 tggcccggcg ggtgcgtcgc cgacctctac gggctcgacc gggtcacgga cggggccagg
     4141 agcgccccgt gacgaggact caggcgtacg tatagaactg tgtgtggtcc agcatgctct
     4201 gcggggtcgt gttcggccag ggcggcatgg tgtcgttcag atcgatcaca tcggtcgtcc
     4261 ccccggcggg gaggtactcg gcactggtcg gatggagtgc gtgccagtcg gcccacagct
     4321 tgtcgacgaa cgcgtggtgc agccagaaca ccggatcgtt cggggagacg ctcgtctcca
     4381 tatgacccca gacccaggtg tgcacccggt tgtgcatgct catgtatccc gcggagccct
     4441 ccagcgtgga gcggaagccc gccgagccgt cacggaaggg cggggaatcg taggtgggca
     4501 tggcgagggc ggcgtcgacg tcggaccggg gcgggagcgt gggaacgcga taggcgagct
     4561 ggcgcgcgag gaaggcgcgg ctgtcgaccg agacgttgat cgtccagttt ccgccggaat
     4621 gcgcgaacgg cccgtccatc acctggtcgt ccagggggcg cccggtaccg ccgaggaagt
     4681 ccggggccca gagcgaggag ccgacggtgt tgtcggcggt ccagtcccag taggggatcg
     4741 agaccgacgg gtcgatcttc tggagttcgt tctcgaaatc gatgaggaac ttacggtgcc
     4801 agggcaggaa cgacggcccg aagtggccca cgtacacacc ggtgttcatg tctatcgaga
     4861 accgcgtgcg gtgggcgagg acgaattcgt cgtacttccc tatgcgtttg acttccagta
     4921 ccgcgtcgac aaaggccttc ttctcggggc ccgtgaggtt cgcctggttc ttccgtacgg
     4981 tcatggctgc tgctcccggt tcaggcggtg gggtcgaacg gaacgacgac ggcgcccttg
     5041 agcgagatga ccgcggtgcg agcggcctcc agcggggtcg cgaatctctc gtagtggttg
     5101 atcgtgctgc tccagccgct cttcccgtgc tgcatcacgg ggagttcgcg gccgtcgatc
     5161 agcacgcggt agccggcgtg ccccgcgtgc ccggcgtgtg cgtgtgcgtc ggtccgggcc
     5221 tcggcgggcg tgccctggat gcggcggccc tcgaagacct cgtcgaagga accgggtgct
     5281 ccggtcccgc cgggtgcgat gccgctgctt tcggcctcgg ccgccgcggt ttccgtggac
     5341 gcggcggcca taccgacgag cgctccgctc gcggcgacgc cgatgacggc cccgagggcc
     5401 tgacggcgag tgggtctggg catggcggaa gtacccccaa aacatggcgg aggcggcccc
     5461 ccggatgagt gtctggggcc gccgtgatca tgtctacacg ggggtgaccg tccgcataag
     5521 cagggaaaaa ggggttggct ctcatatgga cacgtcttca cgtacataga ggggcgaccg
     5581 gccccgcgtc ccgctccgcg ccggtgccgg aggggccgga aggcgcaggt cttgccgtgt
     5641 atgtgccctg cctgtgaagg atcttcggaa tccgtcgcgc tgtcgcccat cggaacgatc
     5701 atccgcgcat ggctggaagt aggagtgggt gctcgctccg gggagaggtt cctgcgcacc
     5761 cgagttcgtc gggggagccg gagcgggccg gacgcgttcc gtccggaggg tgacgtttgt
     5821 gtgcgggccg agcgcccacg ccgtgatcat gtcttcggtc tgtgacagcg ccgtcctgga
     5881 acggcacacg gggccccctg ctgccctcgg cccctcatag tgatcaagga acacccgggc
     5941 ggtcggactc gcttcggtgc cacgaccacg ctcacgacca ccaaggggat ctcatgttcg
     6001 gcaagcgtcg caaggccttc ctcgtctccg cggccctcgt gggcggcgcc ctgctgatga
     6061 cggcgtgcca ggacacggag gacggcgccg cgccgagtgc ctccgccacc gacgtgcccg
     6121 ccgcggacgc ggccgggtcc gacgcctcga agggcaccga cgagggcgcg gagcagggtg
     6181 ccggccagga gcccgacgcg aaggacgggg gcagcgcgga ggggtccggc gccgacgccg
     6241 gcgacggcac gaaggtgccg gtccagcagg cctgcggcgc caacgacatc tcctggagca
     6301 cccggtccga gagccaggcc ggcggctaca tcctgatcat ggcgaaggcc aagcagggga
     6361 tcacctgcat cctgccggcc gcgctcccga cggtggcgtt cggctccgac ggcaccgagg
     6421 cgggccccgc cgagcagtcg gtcggcgacg cggtcacgct gagcggcgcc accaccgcct
     6481 acgccggggt gaacccgaag accaccgaca ccgacggcgg caaggagctg gactccatca
     6541 tcgtcggcgt cggcaacgag gaccccgacc cggtctccct ggaggtcggc accatcacgg
     6601 tcgacaagcc ggtcgtcacc aactggcaca ccgccccggc ggacgccgtt cccgccgcct
     6661 gagagaacgg gcgccgggcc ggggccggtg cgttgatcgc gagacctcga tccggcgtca
     6721 cacggggggc tggtggacgc gggggattcc caggtccggc agttcgaggt ggaagccgcg
     6781 cccgcggccg gtcatggttc cgtcgtagga gaggtcggcc agaccgacgc ccagatgacg
     6841 cagttcctcg acgagcatgc gggcggccgt cgcggcgtgc gcggggtcgt ccatggcgtc
     6901 ggggaagcgg cgctgccaga ccgaggggga gggaacccag ccgttccgcg tcaggcgggc
     6961 ggggtcggcc gcgtccggcc ggtgcaggta gccggtggct tcgatgacga gacccagatc
     7021 ctggctccag cactggacga agcgctgggg atcgtcggcg gatcccaggt ggaggatgaa
     7081 cctgccgggg aagaacgggg tgtcgtggtc gagggagcgc agggcttcgg acaggcgctt
     7141 ctccacctct tcccacgtgg tgccgggcgc gggcggcgcg gcgggcgcgc tcggcagacc
     7201 ggcgagctcg gcggcacgcc agcggccacg ggagtagccg cctccggcgg cgtcggtgtc
     7261 cgcgtcggga ggcaggagtt cgaggctcac ctcgccctcg tcgtgcgtgt gcacgaggag
     7321 gcagacgccg gggcggcgcc aaagcatcca cgggccggag tcgcctcccc acatcgtggg
     7381 cgggcccagc tcctcctcca tgacccacca ggcggcgcga acgaggcccg cggcggtggc
     7441 tgtgtcctcg gcgggcaggt cgacgacgcg tacgttcagc ttgcgcacct gatcgggttc
     7501 agaggcgtcg gcgatcaccg tgccgtagcc gccccagggc cccttccggg gagcgaatct
     7561 gcgccagacc cggcccacga cctccttggg ctcccggagt tcccagccca gctccgaggt
     7621 ggcctgctcg aactccgaca ggctccacga gccgaagcgg agcggcgtga cctcgcggac
     7681 cagctcgcgg aaggtgtcgt cgtcgagcca attgccccgg gggatcgtgt tctccatccg
     7741 gcagagcgta gtgggcggtg cgcacggggg ccgatgggcc gacgttgacg gaacggtccc
     7801 gggaggcgac gctcacccca ccaggagaga gaggtacagc atggaggcac atgcctcgga
     7861 actcgtgttc gggcagaagg tgttggaggc ccagccgttc agcgtcctgc tgggcgcacg
     7921 gctgacggcc ttcggcgacg gagcggcagt cctggaactg gacgtacggg acgagctgcg
     7981 ccagcagaac ggcttcgttc acggaggtgt cctgagctac gcggccgaca acgccctgac
     8041 cttcgcggcg ggtgcggccg tcggcccgtc cgtcctgacg gccggtttca ccatcgacta
     8101 tctgcgtccg gcccggggcg aggtgctgcg cgcgcgggcg caggtggtcc gacccggccg
     8161 tacccgggtc gtctgccgct gcgatctgct gatggtggac gccgcagggg tggaaaccct
     8221 ctgcgcggtg gcccagggca cgatcgccgt cgccggggac tccggaagcc gaccgccgca
     8281 ctcgcccctg tgagcgggcg gggaggcgtg gcccgccggg ccacgcctcc ccgtcacgcc
     8341 gtgcctcaga tgccgcagct gggcgcggac cagaagcggc cgctgccccc ggcgacgtgg
     8401 gtgtggtcgt tgtggccggg gtagcccggg ccgaggatct cggtgaagcc gtggttgcgt
     8461 gccgcctggg cgaggccgca gaagccctgg gagcccgcgc cgaggtccgc cgcgtgcccg
     8521 tacatgtggc ggctgttggc ggctccgccg accgcgctgt tgcaggacac gctgcggaag
     8581 ccgccgttga cggtgatggg ccggtccccc atcgcgtgcc gcatggcctg cagcttccac
     8641 atggtcacca gggcgttggc gcgcgcggtg gcggcgctga ccttgccgcc ggaccagtcg
     8701 gagttgcagc ggttcagctc ggcgtaggtg aagttgaccg gggtgcagtc gtcgtcctgc
     8761 aaggcgtaga tcttgctgaa cgtctgggcg ccggcgatgc cgtcggccgc caggccgtac
     8821 gcggcctgga agcgctgcac ggctgccttc gtggccgggc cgaacacccc gtcgatggcg
     8881 atctggttgc cggtgccggg atagcccgcg acgcggatct ggagctgacg taccgcctcg
     8941 ccgctggagc cctcctggag attgccgccc caggtgtaac aggcgtcggc cgtaccgacg
     9001 ttcccggtcg gggcggaacc gggggcagag ggcggggtcg cggaggcggc tgcggtgccg
     9061 ccgaacgcga gggccgcgcc cgcgagggcc gagaggagga atccgacggg gcgtgatcgc
     9121 atggggctgc acctcattgt cgtcaagctg tccacggggg gttccgagcc tcgggcgcag
     9181 agccggatgt gtcaaggtca tgtcaacttg ctgccatgga gccgggctcc tggcggggtg
     9241 gcaggatgtg aatatggcga tagatcgact gtgggcgggg cttcacggtg acctgcgggc
     9301 agcgaagtca tcggtgtacg acccgagtgg tttcacctgc tcgcagccgg tgcccgaacc
     9361 ggagagcgcc gactacgcgg ctcacgggtt cacgctcgac gggcgggtgg tcgcctttcg
     9421 cgtggccaga accaccccga ccaaggccgg acagttcgtc actgtgtggc agcggtccgc
     9481 ggagggcccg atccggccct tcgacaccga tgacggggtt gacctcttcg tcatcggcag
     9541 ccgcgacaac gggcacttcg ggcagttcgt gttcccacga gaggttctgt gcgagcgcgg
     9601 catcgtgtcc cggaacggcg tgggcgggaa gcggggcttc cgcgtctacc ctccgggcgc
     9661 gatcaccgtc ggtcgtcagg cgcgcgggac tcaggcgtgg caggtgaagt acttcctgga
     9721 cctcggggag gacgggcacg ggcccgtcga cctggtgcgc gcccgggctc tccaccaccc
     9781 gtagcgcacc accggcccgg aagcctttcg gcaccgatga gtcccgggag cccttcaccg
     9841 tggtccgtga aggtactcac cgattccaga ccaacgtgta gcgccagaac agccgtcggc
     9901 gcagccgcgc gccgggcagc acgaggcggg cgtcgcgaac gatgtccgga aaggtcgtgg
     9961 tcgccggtcg ggtcggtgcg gtcatggcgg cggggcgtgg cgctttgcgg cccttgttct
    10021 tgatccaggc catggcgatg ttcgacggaa tggcgacggc gtcgatcagg tggtcgctcg
    10081 gggaccgcgg gcggtagagg ccgacgacga tcagggttcc cccgggtgcc aggcgctcgc
    10141 ggaagcgggt gagcgcgtcg gtcaacggca tgtggtggat ggtggcgacg caggtgatga
    10201 cgtcgtacgg cccggacggt acgtcctcca gcgcgtcgcc gacgaagaag gtcaccgggg
    10261 ccccgggagc ggtgcgctcc cgcgcgcgat cgacgatggc ggggtcggcg tcgatgccgt
    10321 gcaccagctg ggcccgggtg gccagcagcc gggcgaggtc accactgccc gatccgacgt
    10381 ccagggcgcc ggcgcaggat ccatcggtgg tagtgggcgt tgt
//
