LOCUS       NZ_CP096281            10399 bp    DNA     linear   CON 08-JUN-2024
DEFINITION  Streptomyces sp. WAC00303 chromosome.
ACCESSION   NZ_CP096281
VERSION     NZ_CP096281.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. WAC00303
  ORGANISM  Streptomyces sp. WAC00303
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8548838)
  AUTHORS   Meyer,K.J., Tan,S. and Nodwell,J.R.
  TITLE     Genome Sequence of WAC00303 Direct Submission
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8548838)
  AUTHORS   Meyer,K.J., Tan,S. and Nodwell,J.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Biochemistry, University of Toronto, 1 Kings
            College Circle 5207, Toronto, ON M5S 1A8, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-MAR-2022
            Assembly Method                   :: Flye v. 2.8.1
            Assembly Name                     :: WAC00303
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58.0x
            Sequencing Technology :: Oxford Nanopore; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023210535.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 05:21:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,823
            CDSs (total)                      :: 7,731
            Genes (coding)                    :: 7,293
            CDSs (with protein)               :: 7,293
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 438
            CDSs (without protein)            :: 438
            Pseudo Genes (ambiguous residues) :: 0 of 438
            Pseudo Genes (frameshifted)       :: 228 of 438
            Pseudo Genes (incomplete)         :: 241 of 438
            Pseudo Genes (internal stop)      :: 26 of 438
            Pseudo Genes (multiple problems)  :: 54 of 438
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:23:54
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1509035
            Orig. end                         :: 1519434
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096281.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Justin Nodwell, University
            of Toronto.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="8"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(315..1415)
                     /locus_tag="MWG59_RS07050"
                     /old_locus_tag="MWG59_07050"
     CDS             complement(315..1415)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123619.1"
                     /locus_tag="MWG59_RS07050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_07050"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_097935814.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 5.3e-17, bitscore:
                     60.9, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDQEEQRVLRVKGRVLVGPDEVRDELWAVGGRITYERPPGADRA
                     VTVRGWALPGLVDAHCHVGLDRHGPVDAATAEKQALTDREAGTLLIRDAGSPSDTRWID
                     DREDLPKIIRAGRHIARTRRYIRNYAHEIEPGDLVAYVAREARRGDGWVKLVGDWIDRD
                     AGDLTACWPRGEVEAAIAEAHRLGARVTAHCFAEESLTDLVEAGIDCIEHATGLTEDTI
                     PLFAERGVAIVPTLVNIATFPDLAAGGEGKFPRWSAHMRQLYDRRYDTVRAAYDAGIPV
                     YAGTDAGGSLAHGLVAGEVAELVKAGIPPLEALSATAWGARRWLGRPALEEGAPADLVV
                     YDEDPRADVRVLAAPRHIVLNGRVTG"
     gene            1547..2653
                     /locus_tag="MWG59_RS07055"
                     /old_locus_tag="MWG59_07055"
     CDS             1547..2653
                     /NRPS_PKS="Domain: Aminotran_5 (17-282). E-value: 2.6e-28.
                     Score: 90.9. Matches aSDomain:
                     nrpspksdomains_MWG59_RS07055_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018957694.1"
                     /locus_tag="MWG59_RS07055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_07055"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_248584398.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.7e-21, bitscore:
                     75.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLPPLTADHFAAIERRVARLLATEQDVVITQGEALLPLE
                     GCIRGGARPGSTALNVVTGPYGQAFGNWLRDCGAEVVDLTVPFHAAVTADQVAEALAEH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSARAWERIAANPAAPRRSYLSLLDWKERWIDGGRTALPHAP
                     AQLEMLGLEACVARIEDEGLDTVMTRHASAAAATRAGAVALGGGLEPYVHEARDAAPVA
                     TTLRAPAGTDAAALVTAALAADPALPLVAGGGALSKEMIRVNHYGADATRGAVLSSLAA
                     LGAVLTDAGRRVDIEAARRAVSETWPSL"
     aSDomain        1598..2392
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MWG59_RS07055_Aminotran_5.1"
                     /evalue="2.60E-28"
                     /label="MWG59_RS07055_Aminotran_5.1"
                     /locus_tag="MWG59_RS07055"
                     /protein_end="282"
                     /protein_start="17"
                     /score="90.9"
                     /tool="antismash"
                     /translation="AIERRVARLLATEQDVVITQGEALLPLEGCIRGGARPGSTALNVV
                     TGPYGQAFGNWLRDCGAEVVDLTVPFHAAVTADQVAEALAEHPEIDFVSLVHAEAATGN
                     TNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDAWGVDLCVIGAQKAMGGPAGVSAVS
                     VSARAWERIAANPAAPRRSYLSLLDWKERWIDGGRTALPHAPAQLEMLGLEACVARIED
                     EGLDTVMTRHASAAAATRAGAVALGGGLEPYVHEARDAAPVAT"
     misc_feature    2673..2691
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.85"
                     /tool="antismash"
     misc_feature    complement(2675..2688)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.47"
                     /tool="antismash"
     misc_feature    2679..2694
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.25"
                     /tool="antismash"
     misc_feature    complement(2706..2722)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.98"
                     /tool="antismash"
     misc_feature    2780..2797
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.75"
                     /tool="antismash"
     misc_feature    complement(2782..2797)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    2792..2807
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    2926..2938
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    complement(2938..2952)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.51"
                     /tool="antismash"
     gene            2963..3493
                     /gene="ectA"
                     /locus_tag="MWG59_RS07060"
                     /old_locus_tag="MWG59_07060"
     CDS             2963..3493
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123622.1"
                     /locus_tag="MWG59_RS07060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_07060"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_097935926.1"
                     /transl_table=11
                     /translation="MTAAPADFARARSEFLSIDAPRVEDGAAIWRIARDSQVLDLNSSY
                     SYLLWCRDFAATSAVARGENGEPIAFVTGYVRPDRPQTLVVWQVAVDQAHRGKGLAAAL
                     LDSLTARVAADQGLSSVETTITPDNTASDRLFTSYAQRHDVALEKEVLFDGELFPEETH
                     LPEVLYRIGPFAT"
     gene            3652..4914
                     /gene="ectB"
                     /locus_tag="MWG59_RS07065"
                     /old_locus_tag="MWG59_07065"
     CDS             3652..4914
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-363). E-value: 7.4e-72.
                     Score: 234.1. Matches aSDomain:
                     nrpspksdomains_MWG59_RS07065_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="MWG59_RS07065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_07065"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_097935925.1"
                     /sec_met_domain="Aminotran_3 (E-value: 9.6e-77, bitscore:
                     257.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRGWPAVFDRAQGARLTDEDGHSY
                     LDFFAGAGSLNYGHNNPVLKRALLDYIERDGITHGLDMATTAKRAFLETFQNVILRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALQELCHRQDMLLIVDDIQMGCGRTGGFFSFEEAGIVPDIVTLSKSISGYGMPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTAAAVLDAYWADGQMEKQTLARGEQVEQTLL
                     AICAEEPTAQFRGRGLVWGMEFEDKARASAVCARAFELGLLLETSGPQSEVVKLLPPLT
                     ITPEELDEGLRTLARCVRETA"
     aSDomain        3736..4740
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MWG59_RS07065_Aminotran_3.1"
                     /evalue="7.40E-72"
                     /label="MWG59_RS07065_Aminotran_3.1"
                     /locus_tag="MWG59_RS07065"
                     /protein_end="363"
                     /protein_start="28"
                     /score="234.1"
                     /tool="antismash"
                     /translation="FDRAQGARLTDEDGHSYLDFFAGAGSLNYGHNNPVLKRALLDYIE
                     RDGITHGLDMATTAKRAFLETFQNVILRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALQELCHRQDMLLIVDDIQMGCGR
                     TGGFFSFEEAGIVPDIVTLSKSISGYGMPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAVLDAYWADGQMEKQTLARGEQVEQTLLAICAEEPTAQFRGRGLVWGMEFE"
     gene            5001..5399
                     /locus_tag="MWG59_RS07070"
                     /old_locus_tag="MWG59_07070"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045195.1"
                     /locus_tag="MWG59_RS07070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_07070"
                     /product="ectoine synthase"
                     /protein_id="WP_007450483.1"
                     /sec_met_domain="ectoine_synt (E-value: 9.8e-54, bitscore:
                     179.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFSDIENTDRHVKAASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCTEGEAELTNDETGETHWITPGTMYLLDGHERHTMRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEA"
     gene            5406..6299
                     /gene="thpD"
                     /locus_tag="MWG59_RS07075"
                     /old_locus_tag="MWG59_07075"
     CDS             5406..6299
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156966.1"
                     /locus_tag="MWG59_RS07075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_07075"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_097935924.1"
                     /sec_met_domain="PhyH (E-value: 1.1e-37, bitscore: 129.2,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTEVRADLYPSRGAAEMTTPRQDPVIWSAPGAPGPVAAKDLQGY
                     EHDGFLTVDQLIAPDEVAVYQAELNRLISDPAVRADERSIVEKQSQNVRSVFEVHRISE
                     VFAGLVRDERVVGRARQILGSDVYVHQSRINVKPGFGATGFYWHSDFETWHAEDGLPNM
                     RTVSVSIALTENFDTNGGLMIMPGSHKTFLGCAGETPKDNYKKSLQMQDAGTPSDEALT
                     KMADRHGIRLFTGKAGSATWFDCNAMHGSGDNITPYARSNVFIVFNSVENAAQEPFAAP
                     IRRPEFIGARDFTPVK"
     gene            6401..7438
                     /locus_tag="MWG59_RS07080"
                     /old_locus_tag="MWG59_07080"
     CDS             6401..7438
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010070337.1"
                     /locus_tag="MWG59_RS07080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_07080"
                     /product="VOC family protein"
                     /protein_id="WP_248584399.1"
                     /transl_table=11
                     /translation="MTSHVRHITIDCADAYALGAFWAQVLGAPLSDEDFPGDPEALVEA
                     PGAAILFVQVDEKKQTKNRVHLDVQPQDRSRDEEVERLLALGATLVGDHRRPNGRGWAT
                     LADPEGNEFCVECSAAERAALTGTRLPVTADDVTSAVRLAVDVLAGAPADRWDAPAGSL
                     DWTCWETVEHLSDDLFAYAVQLGPRTPPLDRDVPYRWAPERQGGPHNAVFADRDAGPAG
                     LLATLEASGALLASMARTTPPEVRSYHGYGISDPEGFAAMGVVETLVHTYDLAEGLGLD
                     WSPSPALCDRVLARLFPDAPAGGDRWTVLLWATGRAELPDHPRRTSWRWDGRPREDAQT
                     ESSAG"
     gene            complement(7414..8484)
                     /locus_tag="MWG59_RS07085"
                     /old_locus_tag="MWG59_07085"
     CDS             complement(7414..8484)
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969934.1"
                     /locus_tag="MWG59_RS07085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_07085"
                     /product="alkene reductase"
                     /protein_id="WP_248584400.1"
                     /transl_table=11
                     /translation="MTTAFDPIDLSGAQLANRIALAPMTRSRAGEGGTATELTATYYAQ
                     RASAGLVITEGIQPSVVGQGYPTTPGLHSAEQVASWRKVTDAVHAEGGRIFAQIMHAGR
                     IGHPVLLPDGLVPVSASPVKAEGQVYTHVGPKDFVEPRELTDAEIHATVADFVTASRNA
                     VEAGFDGVELHGANGYLIQQFLAPNTNRRTDAWGGSDEARIRFAVEVVKAVAAEIGPER
                     TALRISPGNPYNDIDEPAPEATYTALVQAIEPLGIAYLHILEAAPIRELTLALRKAFSG
                     TLVLNVHSDGATGPGDHTVIDDGIADLISYGALFLANPDLPARLKAGGPFNTPDPSTFF
                     GGDAKGYTDYPALDSV"
     gene            8636..9142
                     /locus_tag="MWG59_RS07090"
                     /old_locus_tag="MWG59_07090"
     CDS             8636..9142
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007450490.1"
                     /locus_tag="MWG59_RS07090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_07090"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_248584401.1"
                     /transl_table=11
                     /translation="MTTGRATPAEDACTNALPMAALSGPVSHAMSRVARLHRTAAGKAL
                     KGVDLYPGQELLMMHLWDRGAVRQTELIRALDLDPSTVTKMLQRLEQTGHVRRRPDPAD
                     RRAVLVEATDESCALHTAVRDAWGSLEEHTLAGLDPAERAELVRLLAKVEENLCRETAD
                     CPDRC"
     gene            complement(9159..10217)
                     /locus_tag="MWG59_RS07095"
                     /old_locus_tag="MWG59_07095"
     CDS             complement(9159..10217)
                     /NRPS_PKS="Domain: Aminotran_5 (19-346). E-value: 1.5e-24.
                     Score: 78.6. Matches aSDomain:
                     nrpspksdomains_MWG59_RS07095_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012381431.1"
                     /locus_tag="MWG59_RS07095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_07095"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_248584402.1"
                     /sec_met_domain="Aminotran_5 (E-value: 4.4e-17, bitscore:
                     60.9, seeds: 34, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_1_2 (E-value: 5.2e-05, bitscore:
                     21.4, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTILAPIRAAAVEFAPEVTYLNTSSWGLLPRRTIAAVKALADENA
                     AGRRVGAGSFDAVEAARAGFARLAGVRPDRVATGSSVTVHVGLIAASLPPGAEVLCPEG
                     EFSSVVSPFALRGDLRMRYAPLDGLAAAVRPSTSLVAFSAVQSADGRVADLAAVRAAAA
                     AHGARTLLDGTQAAGWFPLEAGAYDYTVTGGFKFLLCPRGTSFLTVTEEAQDTLPPLFA
                     GWVSAGAPWTSNYGPLERLAPTARGFDEPPAFLSYHGAEHSLDLLAEVGADALYAHATG
                     LAARLRAGLAKLGHATVPGESAIVSVPGLESRQPDLVKAGVAVSAPAGNLRISCHLYNT
                     EADVDRALEVLA"
     aSDomain        complement(9180..10160)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MWG59_RS07095_Aminotran_5.1"
                     /evalue="1.50E-24"
                     /label="MWG59_RS07095_Aminotran_5.1"
                     /locus_tag="MWG59_RS07095"
                     /protein_end="346"
                     /protein_start="19"
                     /score="78.6"
                     /tool="antismash"
                     /translation="YLNTSSWGLLPRRTIAAVKALADENAAGRRVGAGSFDAVEAARAG
                     FARLAGVRPDRVATGSSVTVHVGLIAASLPPGAEVLCPEGEFSSVVSPFALRGDLRMRY
                     APLDGLAAAVRPSTSLVAFSAVQSADGRVADLAAVRAAAAAHGARTLLDGTQAAGWFPL
                     EAGAYDYTVTGGFKFLLCPRGTSFLTVTEEAQDTLPPLFAGWVSAGAPWTSNYGPLERL
                     APTARGFDEPPAFLSYHGAEHSLDLLAEVGADALYAHATGLAARLRAGLAKLGHATVPG
                     ESAIVSVPGLESRQPDLVKAGVAVSAPAGNLRISCHLYNTEADVDR"
     misc_feature    complement(10253..10266)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.28"
                     /tool="antismash"
ORIGIN
        1 agatggtgga acccccacaa gagacatgga gccgccggca cagtccaatg ggggacatcg
       61 cctgcgccga cggcctcgac tccgtgaatc tttacgcact gagggtgaac tggcggatac
      121 ctggcgtgag ttcaccccaa aggggggttt ccgcgcgcat attcgaatct ttcggcacgt
      181 gcgttcgctg atgtcactcg tttcacccgg cgtgagccgt acggagcgcg ctcgcgccgg
      241 agcatgcgca gaggtgcatc aacgagtccg ggaccgtatc cgtcactgct cgtcaacaca
      301 gcaccgcgcc gctctcagcc ggtcacccgc ccgttcagca cgatgtggcg aggcgccgcc
      361 agcacccgga cgtccgcgcg cgggtcctcg tcgtacacga ccaggtccgc cggagcgccc
      421 tcctccagcg cgggccgccc cagccagcgc ctggcccccc acgcggtggc ggagagggcc
      481 tccaggggcg ggatgcccgc cttgacgagt tcggcgacct cgcccgcgac cagcccgtgc
      541 gccagggagc cgcccgcgtc ggtgcccgcg tagacgggga tcccggcgtc gtacgcggcg
      601 cgcacggtgt cgtagcggcg gtcgtacagc tgccgcatat gggccgacca gcgggggaac
      661 ttgccctcgc cgcccgccgc gagatcgggg aacgtggcga tgttgaccag cgtcgggacg
      721 atcgccaccc cccgctcggc gaagagcggg atggtgtcct cggtgaggcc ggtggcgtgc
      781 tcgatacagt cgatgcccgc ctccaccagg tcggtgagcg actcctcggc gaagcagtgc
      841 gcggtgacgc gggcgccgag ccggtgcgcc tcggcgatgg ccgcctcgac ctcgccgcgc
      901 ggccagcagg cggtcaggtc cccggcgtcg cggtcgatcc agtcccccac cagcttgacc
      961 cagccgtccc cgcgccgggc ctcccgggcc acgtacgcga cgaggtcgcc gggttcgatc
     1021 tcatgggcgt agttgcggat gtagcggcgg gtcctggcga tgtggcggcc tgccctgatg
     1081 atcttcggaa ggtcctcgcg gtcgtcgatc caccgggtgt cggagggcga tcccgcgtcc
     1141 cggatcaaca gggttccggc ctcccggtcg gtgagcgcct gcttctcggc ggtcgccgcg
     1201 tcgaccgggc cgtgccggtc caggcccaca tggcagtgcg cgtcgaccag tccgggcagc
     1261 gcccatcccc tgaccgtcac cgcgcggtcg gcgccgggcg gccgctcgta ggtgatccgc
     1321 ccgcccacgg cccacagttc gtcccgcacc tcgtccgggc cgacgagcac ccggcccttc
     1381 acccgcagca cccgctgctc ctcctgatca ctcatacgcg gcactgtacg aggccgtcgg
     1441 taggctgctg gggcccgtgg acgatcccgc cgcccgcccg gaaggcggga acgtttcacc
     1501 ggcgcctgcc catggacccg cgaacgaacc cgaagagagc cccaccgtga cgcacccctt
     1561 cctcgacctc cccccgctca ctgccgacca cttcgcggcg atcgagcggc gcgtggcccg
     1621 gctcctcgcc acggagcagg acgtcgtcat cacccagggc gaggcgctgc tgccgctgga
     1681 gggctgcatc cgcggcggcg cacggcccgg ctcgaccgcg ctgaacgtgg tcaccggacc
     1741 gtacgggcag gccttcggca actggctgcg ggactgcggc gccgaggtcg tcgacctcac
     1801 ggtgcccttc catgccgcgg tcaccgccga ccaggtcgcc gaggcgctcg ccgagcaccc
     1861 ggagatcgac ttcgtctcgc tggtgcacgc ggaggcggcg accggcaaca ccaacccggt
     1921 cgcggagatc ggcgaggtgg tccgggcgca cggcgcgctg ttcatgctgg acgcggtcgc
     1981 ctcggtgggc gccgagccgc tgctgccgga cgcctggggc gtcgacctgt gcgtgatcgg
     2041 cgcacagaag gccatgggcg ggcccgccgg ggtgtcggcg gtgtcggtga gcgcgcgggc
     2101 ctgggagcgg atcgccgcca acccggccgc cccgcgccgc tcctacctct ccctcctgga
     2161 ctggaaggag cgctggatcg acggcggccg cacggcgctg ccgcacgccc cggcgcagtt
     2221 ggagatgctg ggcctggagg cctgcgtggc gcggatcgag gacgagggcc tggacacggt
     2281 gatgacccgc cacgcctcgg ccgccgcggc cacccgggcc ggtgcggtgg ccctgggcgg
     2341 cgggctggag ccgtacgtcc acgaagcgcg ggacgcggcc ccggtggcga cgacgctgcg
     2401 cgccccggcg gggacggacg cggccgcgct ggtcacggcg gcgctggcgg cggaccccgc
     2461 gctgccgctc gtcgcgggcg gcggggcgct gtccaaggag atgatccggg tcaaccatta
     2521 cggggcggat gcgacgcggg gcgcggtgct gtcctcgctc gcggccctgg gggcggtgct
     2581 gaccgatgcc ggccggcggg tcgatatcga ggctgcccgt agggctgttt cggaaacctg
     2641 gccgagcctc tgatatttct catggggaaa gctgctttat tgaaaagcag cttcccacat
     2701 tcttctgccc gctttcccgt cacctaaacg ggccagtttt ttctggccgc gaacttcatg
     2761 attcggacac atctcgcgag agttacaacc ctgtgaccga ctccacaggg tcggcattat
     2821 gcccgaaaaa ttcaggacaa agcgcatgaa aacgcacctg aatcaccggg aagctcgcgc
     2881 acgcctgata acacaaagaa gccctccacc caaccctcta cgtcgatgca atttcgaaat
     2941 ttgctgggta aattcaattc gcatgaccgc cgcaccagca gattttgccc gtgcccgaag
     3001 cgaattcctc agtatcgatg cgccacgagt ggaggacgga gccgcgatct ggcgcatagc
     3061 ccgcgactcc caggtcctgg acctgaactc ctcgtacagc tacctgctgt ggtgccgtga
     3121 cttcgccgcg acctccgccg tcgcccgtgg cgagaacggc gagcccatcg ccttcgtgac
     3181 gggctacgtc aggcccgacc gcccccagac cctcgtcgtc tggcaggtgg ccgtcgacca
     3241 ggcccaccgc ggcaaggggc tggccgccgc tctgctggac tcgctgaccg cccgggtcgc
     3301 cgccgaccag ggcctgtcgt ccgtggagac gacgatcacg ccggacaaca ccgcatccga
     3361 ccggctgttc acgtcctacg cccagcgcca cgacgtcgcg ctggagaagg aagtgctctt
     3421 cgacggcgag ctgttccccg aagagacgca cctgcccgag gtgctctacc ggataggccc
     3481 gttcgccacc tgagccgccc cggccaccgc accccggtgt acccgacccg tacgccggat
     3541 ccccctgact tttcgcgccg accgaccact cggcgcggtc ccggccgcac ccgtgcggcc
     3601 gggagcactc gaccactgcc cgtcacaccc ccctcacgca ggagatccgc tgtgaccatc
     3661 accccgcccg ccctgagcgt cttcgagacc ctggagtccg aggtacggag ctactgccgc
     3721 ggctggcccg ccgtgttcga ccgcgcgcag ggcgcccggc tgaccgacga ggacggccac
     3781 tcctacctcg acttcttcgc cggggccggc tcgctcaact acggccacaa caacccggtg
     3841 ctgaaacgag cgctgctgga ctacatcgag cgcgacggca tcacccacgg cctggacatg
     3901 gcgaccaccg ccaagcgggc gttcctggag accttccaga acgtgatcct gcggccgcgc
     3961 gatctgccgt acaaggtgat gttccccggc ccgaccggca ccaacgccgt cgaggcggcg
     4021 ctgaagctgg cccgtaaggt caagggccgc gagtcggtcg tctcgttcac caacgccttc
     4081 cacggcatgt cgctgggctc actcgcggtg accggcaacg cgttcaagcg ggccggggcc
     4141 ggcatcccgc tggtgcacgg caccccgatg ccgttcgaca actacttcga cggcacggtc
     4201 cccgacttcc tctggttcga gcgtctcctt gaggaccagg gctccgggct caacaagccc
     4261 gccgcggtga tcgtggagac cgtccagggc gagggcggca tcaacgtcgc ccgcgccgag
     4321 tggctgcgcg cgctgcagga gctgtgccac cgccaggaca tgctgctgat cgtcgacgac
     4381 atccagatgg gctgcggccg caccggcggc ttcttctcct tcgaggaggc cggcatcgtc
     4441 ccggacatcg tcacgctctc caagtcgatc agcggctacg gcatgccgat gtcgctctgc
     4501 ctgttcaagc ccgagctgga catctgggag ccgggcgagc acaacgggac cttccgcggc
     4561 aacaacccgg ccttcgtcac ggccgctgcg gtgctggacg cctactgggc ggacggtcag
     4621 atggagaagc agaccctcgc ccgcggcgaa caggtcgagc agaccctgct ggccatctgc
     4681 gccgaggagc cgaccgcgca gttccgcggc cggggcctgg tgtggggcat ggagttcgag
     4741 gacaaggcgc gcgcctcggc ggtctgcgcc cgcgccttcg agctggggct cctcctggag
     4801 acctcgggcc cgcagagcga ggtcgtcaag ctgctgccgc cgctgaccat cacccccgag
     4861 gagctggacg agggcctgcg cacgctggcc cgctgcgtcc gcgaaacggc ctgacccggc
     4921 actgccgggg caggcggaga agagaggtcc cgggccgcga ggcccgggac gctgtacaga
     4981 gagaaaggca accgctcacc gtgatcgtcc gatcgttcag tgacatcgag aacaccgacc
     5041 ggcatgtgaa ggcggcatcc ggtacctggg agagcaagcg catcgtgctc gccaaggaga
     5101 aggtgggctt ctcgctccac gagaccgtgc tgtacgcggg cacggagacc tccatgtggt
     5161 acgcgaacca catcgaggcg gtgctgtgca ccgagggcga ggccgagctc accaacgacg
     5221 agaccggcga gacgcactgg atcacgccgg gcaccatgta cctgctggac gggcacgagc
     5281 ggcacaccat gcggcccaag accgacttcc gctgcgtgtg tgtcttcaac ccccccgtca
     5341 ccggacggga ggaccatgac gagaacggtg tctacccact gctgacagag gaggcctgaa
     5401 ccaccatgac caccgaagta cgcgccgatc tgtacccctc gcgcggcgcc gccgagatga
     5461 ccactccccg ccaggacccg gtcatctggt ccgcgccggg cgcaccgggt ccggtcgccg
     5521 ccaaggacct ccagggatac gagcacgacg gcttcctcac cgtcgaccag ctcatcgccc
     5581 cggacgaggt cgccgtctac caggcggagc ttaaccggct gatctccgac ccggcggtcc
     5641 gcgccgacga gcgctcgatc gtcgagaagc agtcgcagaa cgtgcggtcc gtcttcgagg
     5701 tccaccggat cagcgaggtc ttcgccggtc tggtccgcga cgagcgggtg gtgggccggg
     5761 cccgccagat cctcggctcg gacgtgtacg tccaccagtc ccggatcaac gtgaagccgg
     5821 gcttcggggc cacgggcttc tactggcact cggacttcga gacctggcac gcggaggacg
     5881 gtctgccgaa catgcggacg gtgtccgtgt cgatcgcgct gaccgagaac ttcgacacca
     5941 acggcgggct gatgatcatg cccggttcgc acaagacgtt cctcggctgt gcgggcgaga
     6001 cgccgaagga caactacaag aagtcgctcc agatgcagga cgccggcacc ccgtccgacg
     6061 aggcgctgac gaagatggcc gaccgccacg gcatcaggct cttcacgggc aaggccggtt
     6121 cggcgacctg gttcgactgc aacgccatgc acggctcggg cgacaacatc accccgtacg
     6181 cgcgcagcaa cgtcttcatc gtcttcaaca gcgtggagaa cgcggcgcag gagcccttcg
     6241 cggctccgat ccgccggccc gagttcatcg gggcgcggga cttcaccccg gtgaagtagc
     6301 gggccgcacg ggcgagacgg ggccgggacg ggtacgccgt cccggccctt cccgggtaac
     6361 tcgctggacc ggggccgggt cagcggagag ggtgtccgcc atgacttccc atgtacgtca
     6421 catcacgatc gactgcgccg acgcctacgc gctcggcgcc ttctgggccc aggtgctcgg
     6481 cgcgccgctc tccgacgagg acttccccgg cgatccggag gctctggtcg aggctcccgg
     6541 cgcggcgatc ctcttcgtcc aggtggacga gaagaagcag accaagaacc gcgtccatct
     6601 ggacgtccag ccgcaggacc gctcccggga cgaggaggtg gagcggctgc tcgcgctcgg
     6661 cgcgaccctg gtgggcgacc accgcaggcc gaacgggcgc ggctgggcga cgctcgccga
     6721 tcccgagggc aacgagttct gtgtggagtg ctccgccgcc gagcgggcgg cgctcaccgg
     6781 gacccggctg ccggtgaccg ccgacgatgt gacctcggcg gtgcggctcg cggtggacgt
     6841 gctggccgga gccccggcgg accgctggga cgcgcccgcg ggaagccttg actggacctg
     6901 ctgggagacg gtggagcacc tgagcgacga tctgttcgcg tacgccgtgc agttgggacc
     6961 ccgtacgcct ccgctggacc gggacgtgcc ctaccggtgg gcgcccgaac ggcagggcgg
     7021 gccccacaac gcggtcttcg ccgaccgtga cgccggtccc gccgggctgc tggccacgct
     7081 ggaggcgagc ggggcgctgc tggcctcgat ggcgcggacg acgccgcccg aggtgcgctc
     7141 gtaccacggg tacgggatct ccgacccgga gggcttcgcc gcgatgggcg tggtggagac
     7201 cctggtgcac acgtatgacc tcgccgaggg cctggggctg gactggtcgc cgtccccggc
     7261 gctctgcgac cgggtgctgg cccggctctt cccggacgcc ccggcgggcg gcgaccggtg
     7321 gaccgtcctg ctgtgggcca ccggccgcgc cgagctgccg gaccacccgc gccgtacctc
     7381 gtggcggtgg gacgggaggc cgcgcgagga cgctcagacg gagtcgagcg ccgggtagtc
     7441 ggtgtagccc ttggcgtcgc cgccgaagaa ggtcgacggg tcgggggtgt tgaacgggcc
     7501 gcccgccttc agccgggcgg gcaggtccgg gttggcgagg aagagcgccc cgtaggagat
     7561 caggtcggcg atgccgtcgt cgatcaccgt gtggtcaccg ggtccggtgg cgccgtcgga
     7621 gtgcacgttg aggacgaggg tcccggagaa ggccttgcgc agggcgaggg tcagctcccg
     7681 gatcggggcg gcctccagga tgtgcagata ggcgatgccg agcggctcga tcgcctgcac
     7741 cagcgcggtg tacgtggcct ccggagcggg ctcgtcgatg tcgttgtacg ggttgccggg
     7801 cgagatgcgc agtgcggtgc gctccgggcc gatctcggcg gcgacggcct tgacgacctc
     7861 gacggcgaag cggatgcggg cctcgtcgga accgccccac gcgtcggtgc gccggttggt
     7921 gttgggggcc aggaactgct ggatgagata gccgttggca ccgtgcagct cgaccccgtc
     7981 gaatccggct tccacggcgt tgcgggaggc ggtgacgaag tcggcgacgg tcgcgtggat
     8041 ctcggcgtcc gtcagctcgc gcggctcgac gaagtccttc ggcccgacgt gggtgtagac
     8101 ctgtccctcg gccttgaccg gggaggcgct gacggggacg agaccgtccg gcagcaggac
     8161 cgggtggccg atgcggcccg cgtgcatgat ctgggcgaag atccggccgc cctcggcgtg
     8221 caccgcgtcg gtgaccttgc gccaggaggc gacctgttcg gcgctgtgca gacccggcgt
     8281 ggtggggtag ccctggccaa cgacggacgg ctggatgcct tcggtgatca cgaggccggc
     8341 cgaggcgcgc tgggcgtagt acgtggcggt gagctcggtg gcggtgccgc cctcaccggc
     8401 ccggctgcgg gtcatcgggg ccagcgcgat gcggttggcc agctgggcgc cggacaggtc
     8461 gatcgggtcg aaagcggtgg tcatgaaggc tcccaagaaa gtatgtggtc ggccaagcat
     8521 cggcgacagc ggcaactgta acccattgat tggtcgacca aggtaaagtt gcgggaaacc
     8581 gtcgggcatc atcgagagcc gccgcctgca accccgagga ccccaggagc ccgccatgac
     8641 caccggccgc gccacccccg cggaagacgc ctgtacgaac gcgctgccca tggcggcgct
     8701 cagcgggccc gtcagccacg ccatgtcccg ggtggcccgg ctgcaccgga cggcggcggg
     8761 caaggcgctc aagggggtgg acctctaccc ggggcaggag ttgctgatga tgcatctgtg
     8821 ggaccggggc gcggtccgtc agacggagct gatcagggcc ctggacctgg acccgtccac
     8881 ggtcacgaag atgctccagc ggctggagca gaccgggcat gtgcgccgcc gccccgaccc
     8941 ggccgaccgg cgggccgtgc tggtcgaggc gacggacgag agctgcgcgc tgcacacggc
     9001 ggtccgggac gcctggggca gcctggagga gcacacgctc gcgggcctcg acccggccga
     9061 gcgcgcggag ctggtccggc tgctggcgaa ggtcgaggag aacctctgcc gggagaccgc
     9121 ggactgcccg gaccggtgct gaaccgcacc cgtacggatc aggccaggac ctccagcgcc
     9181 cggtccacat cggcctcggt gttgtagagg tggcaggaga tacgcagatt cccggccggg
     9241 gccgagacgg cgacaccggc cttcaccagg tcgggctgac gactctccag acccggcacg
     9301 gagacgatcg ccgactcccc gggaaccgtc gcgtgcccga gcttcgccag ccccgcccgc
     9361 agccgggccg cgagcccggt ggcgtgcgcg tacagggcgt cggcgcccac ctcggcgagc
     9421 aggtccaggg agtgttcggc cccgtggtac gaaaggaagg cgggcggctc gtcgaagccg
     9481 cgggcggtgg gggcgaggcg ctccagcggg ccgtagttgc tcgtccaggg agcgccggcg
     9541 gagacccagc cggcgaagag gggcggcagg gtgtcctgcg cctcctcggt gacggtcagg
     9601 aacgaggtac cgcgcggaca gagcaggaac ttgaagccgc cggtgacggt gtagtcgtac
     9661 gcccccgcct ccagcgggaa ccagcccgca gcctgggtgc cgtccagcag ggtccgggcc
     9721 ccgtgcgccg cggcggccgc gcggacggcg gcgagatcgg ccacccggcc gtcggccgac
     9781 tgcacggcgg agaaggcgac gagggaggtc gaggggcgca cggccgcggc gaggccgtcg
     9841 agcggcgcgt accgcatgcg cagatcgccg cggagcgcga acgggctgac cacggaggag
     9901 aactcgccct cggggcagag gacttcggcg cccggcggca gtgaggcggc gatcagaccg
     9961 acgtggacgg tgacggaact gccggtggcg acccggtccg gacggacccc ggcgagccgg
    10021 gcgaagccgg cccgggcggc ctccaccgcg tcgaagctgc ccgctcccac ccggcgcccg
    10081 gcggcgttct cgtcggccag ggccttgacg gcggcgatcg tccggcgggg cagcaggccc
    10141 cagctggagg tgttgaggta ggtgacctcg ggcgcgaatt ccacggctgc cgcccggatg
    10201 ggcgcaagga tcgtcatagg tccaccctga agcccccttg acccaaagtc aatcacggag
    10261 agtcaagggg gcatcaaagg aatgcttata gtcgagttct cgggcgggct cagctccgcg
    10321 gaacctcgca ggacccgtcc gtgtcgcagg aagccgcatc cccgccgacg gcggtgagag
    10381 cggtgacggg acggtcctt
//
