LOCUS       NZ_CP096281            10945 bp    DNA     linear   CON 08-JUN-2024
DEFINITION  Streptomyces sp. WAC00303 chromosome.
ACCESSION   NZ_CP096281
VERSION     NZ_CP096281.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. WAC00303
  ORGANISM  Streptomyces sp. WAC00303
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8548838)
  AUTHORS   Meyer,K.J., Tan,S. and Nodwell,J.R.
  TITLE     Genome Sequence of WAC00303 Direct Submission
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8548838)
  AUTHORS   Meyer,K.J., Tan,S. and Nodwell,J.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Biochemistry, University of Toronto, 1 Kings
            College Circle 5207, Toronto, ON M5S 1A8, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-MAR-2022
            Assembly Method                   :: Flye v. 2.8.1
            Assembly Name                     :: WAC00303
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58.0x
            Sequencing Technology :: Oxford Nanopore; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023210535.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 05:21:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,823
            CDSs (total)                      :: 7,731
            Genes (coding)                    :: 7,293
            CDSs (with protein)               :: 7,293
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 438
            CDSs (without protein)            :: 438
            Pseudo Genes (ambiguous residues) :: 0 of 438
            Pseudo Genes (frameshifted)       :: 228 of 438
            Pseudo Genes (incomplete)         :: 241 of 438
            Pseudo Genes (internal stop)      :: 26 of 438
            Pseudo Genes (multiple problems)  :: 54 of 438
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:23:54
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 40582
            Orig. end                         :: 51527
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP096281.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Justin Nodwell, University
            of Toronto.
FEATURES             Location/Qualifiers
     region          1..10945
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10945
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10945
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5945](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5945)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    8..22
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
     gene            complement(1026..2066)
                     /locus_tag="MWG59_RS00295"
                     /old_locus_tag="MWG59_00295"
     CDS             complement(1026..2066)
                     /EC_number="1.1.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (19-268). E-value: 4.6e-12.
                     Score: 37.6. Matches aSDomain:
                     nrpspksdomains_MWG59_RS00295_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019761872.1"
                     /locus_tag="MWG59_RS00295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_00295"
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="WP_097935166.1"
                     /sec_met_domain="ADH_N (E-value: 4e-28, bitscore: 96.3,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 1.5e-22, bitscore:
                     78.8, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVAAYAAPRAKAPLERTTIERRPVGEFDVLIDIKFAGICHSDI
                     HQARDGWGEGIFPMVPGHEIAGIVAETGSGVTRFKVGDRVGVGCMVDSCGKCDACLMGR
                     EQHCAEGNTQTYNALDRSGEPTYGGYSTHLVVAETFALSIPEGIALDEAAPLLCAGITT
                     YSPLKRWGAGPGKKVAVVGLGGLGHMAVKIAHALGAEVTVLSQSLRKKDDGLKLGADHY
                     YATSDPRTFEELAGTFDVILSTVSAPLDFGAYLGLLRTDGTLVNVGAPEEPISLNLFSL
                     ILGNKSIAGSAIGGIKETQEMLDFCAVHGLGAEIEVIDAGQVNEAYERILASDVRYRFV
                     IDTATI"
     aSDomain        complement(1263..2009)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MWG59_RS00295_PKS_ER.1"
                     /evalue="4.60E-12"
                     /label="MWG59_RS00295_PKS_ER.1"
                     /locus_tag="MWG59_RS00295"
                     /protein_end="268"
                     /protein_start="19"
                     /score="37.6"
                     /tool="antismash"
                     /translation="TIERRPVGEFDVLIDIKFAGICHSDIHQARDGWGEGIFPMVPGHE
                     IAGIVAETGSGVTRFKVGDRVGVGCMVDSCGKCDACLMGREQHCAEGNTQTYNALDRSG
                     EPTYGGYSTHLVVAETFALSIPEGIALDEAAPLLCAGITTYSPLKRWGAGPGKKVAVVG
                     LGGLGHMAVKIAHALGAEVTVLSQSLRKKDDGLKLGADHYYATSDPRTFEELAGTFDVI
                     LSTVSAPLDFGAYLGLLRTDGTLVNVG"
     misc_feature    2129..2144
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.13"
                     /tool="antismash"
     gene            2229..3155
                     /locus_tag="MWG59_RS00300"
                     /old_locus_tag="MWG59_00300"
     CDS             2229..3155
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459219.1"
                     /locus_tag="MWG59_RS00300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_00300"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_248583832.1"
                     /transl_table=11
                     /translation="MDDQPEPAPEPLDRRAELSEFLRTRRAKLRPGDVGLPEFGRHRRV
                     PGLRREELAQLAGVSVAYYTRLEQGNGRNVSAEVLDAIARALRLTDAESAHLTHLAKPA
                     RHKKKRRPARVQRVRKGLLYLLDSMDGIPAYVTGARSDVLAWNAMAAAVFGDWGALPPA
                     ERNWARLVFLSPAYRDLFVNWDSKASDMVSYLRLYAGCQPDDPELSALVGELSVKSDEF
                     RRLWATHNVKEKGHGVKLLRHPLVGELTLSYETLNLPDDEEQHLVTYHAEPGSESAQSL
                     RLLASWGADAGLTDVIGDAGRGPADER"
     gene            complement(3115..3861)
                     /locus_tag="MWG59_RS00305"
                     /old_locus_tag="MWG59_00305"
     CDS             complement(3115..3861)
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459221.1"
                     /locus_tag="MWG59_RS00305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_00305"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_097935164.1"
                     /transl_table=11
                     /translation="MADENFLHPRLAALYDPLDPDRGDLEPYVRLAEESGARRVLDIGC
                     GTGVFALMLADRGIEVVGVDPARASLDVARAKPGGDRVRWIHGDAAALPPLRVDLVTLT
                     ANAAQEIVDPSDWEATLHGAHEALRPGGQLVFETRDPARRAWETWNRESSHRVTEIPDV
                     GPVETWVEVTEIVGPLVSFRWTYVFRRDGQVLTSDSTLRFRERQEAEADLAAQGFVVRE
                     VRGAPDRPGRELVLIARRPGPAPRRL"
     misc_feature    4062..4073
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.43"
                     /tool="antismash"
     gene            complement(4119..4988)
                     /locus_tag="MWG59_RS00310"
                     /old_locus_tag="MWG59_00310"
     CDS             complement(4119..4988)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012382293.1"
                     /locus_tag="MWG59_RS00310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_00310"
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_097935163.1"
                     /transl_table=11
                     /translation="MILVTGATGAVGREVAGQLAAAGPVRILARRPERLTVRGEGVEVV
                     EGEYGDRAALDRALRGVASVFLVTNSPTEPDDERVAAAAAAAGVRHLVKLSMMAVEEPG
                     ADDFITRRQRENEQAVRESGVPWTFVRPRTFMSNTLSWAPGIRSTGVVRALYGDSPVAC
                     VDPRDVAAVAVAVLTGTGHEGRAYAVSGPEAITAREQTAQLSRVLERPLRFEELGVDAA
                     RTALLAKYPPPVAEAFLQSAERQRAGAKAAVVPTIQDLTGRPARPYRIWSAEHAEAFAP
                     EAGRFAGE"
     gene            complement(5001..5945)
                     /locus_tag="MWG59_RS00315"
                     /old_locus_tag="MWG59_00315"
     CDS             complement(5001..5945)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971211.1"
                     /locus_tag="MWG59_RS00315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_00315"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_097935162.1"
                     /sec_met_domain="AfsA (E-value: 1e-25, bitscore: 88.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEAAVLIDPVPTMDAEAEVVHPVEIDMVHRTRPEDAFPRNWVRL
                     GHDRFSVGAVLPHDHPFFAPVGDDRHDPLLVAEAMRQAAMLAFHAGYGIPVGYQFLMSG
                     LDYVCHQEHLTVGGAPTEIDVEVFCSDLKWRGGLPAQGRVGWAVHRDGRLAATGMGETR
                     FSTPKAYRRMRGEAPAEGISIPQTAPVSPVLAGRARAEDVVLSETGRDGVWELRVDTRH
                     PTLFQRPNDHVPGMLLLEAARQAAWLAVGPAGIVPAEARTRFHRYSEFGSPCWIGAVVL
                     PETAEDTVTVQVTGHQDGEAVFSTILSGPRADG"
     gene            complement(6470..7081)
                     /locus_tag="MWG59_RS00320"
                     /old_locus_tag="MWG59_00320"
     CDS             complement(6470..7081)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459226.1"
                     /locus_tag="MWG59_RS00320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_00320"
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="WP_248583833.1"
                     /transl_table=11
                     /translation="MTNALTTAAAGFGTGLSLIVAIGAQNALVLRQGVRRDGVLAVVGI
                     CALSDAVLIALGVAGVGALVVAWPSALTVVGLLGGVFLIGYGLLAARRVMRPSALHAGD
                     APAASRRGVVLTCLAMTWLNPHVYLDTVFLLGSVAADRGPLRWTFGLGAVLASVCWFAA
                     LGFGARLLSGVLARPSAWRVLDGLVAVMMIGLGAKLALTA"
     misc_feature    complement(7104..7118)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.23"
                     /tool="antismash"
     misc_feature    complement(7139..7153)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.4"
                     /tool="antismash"
     gene            7149..8069
                     /locus_tag="MWG59_RS00325"
                     /old_locus_tag="MWG59_00325"
     CDS             7149..8069
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1031: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971209.1"
                     /locus_tag="MWG59_RS00325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_00325"
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="WP_248583834.1"
                     /transl_table=11
                     /translation="MMSELPLDQVRTLLAVVDEGTFDSAANALRLTPSAVSQRVKALEQ
                     RTGRVLLMRTKPVRPTESGEVVVRFARQLARLEHDAQTALGMSGPGEPTILPIAVNSDS
                     LATWFLPALRRVPEELGLCYELRREDQDHTAALLREGLAMAAVTSSPEAVTGCSVRRLG
                     RMRYLPVASPGFADRWLGRREGTALRELIADAPVIGFDRRDDLQDAFVRRLGPGPRASA
                     RRHLVPTSEGFANAVATGMGWGMVPEVQAEPLLSSGRLVRLTPDHTVDVPLYWQQWKLE
                     SPALAAVSEAVAAEAAEALDGDWPD"
     misc_feature    8138..8156
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.41"
                     /tool="antismash"
     misc_feature    8147..8162
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 19.47"
                     /tool="antismash"
     gene            8178..9425
                     /locus_tag="MWG59_RS00330"
                     /old_locus_tag="MWG59_00330"
     CDS             8178..9425
                     /GO_function="GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042: O-methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459230.1"
                     /locus_tag="MWG59_RS00330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_00330"
                     /product="acetylserotonin O-methyltransferase"
                     /protein_id="WP_248583835.1"
                     /transl_table=11
                     /translation="MTDRHGQEAGNGREQPMDWAARGAVARLVLGQMATQALGTAVRLG
                     VFDRIGPDELSAEALAGSLGTHPQATLRLLRALAGLQLLSETEPSAFRTTTAGDALRAD
                     TPGTMVAMARMFTDPVMLRGWDLLDESVRTGETTFDTVFGTDFFGHLKEHPELSASFNE
                     AMSQGTRLTATTVPHHYDFGRFHTLVDIGGGDGTLLASVLREHPKLRGVLFDTAEGLAQ
                     APRACFESSAVRPEGGLGGVWCVRSQGGGSPRYWTYRGDPDNAASVRARRRRAGGTFET
                     RPRRLAQEGLTERVALETGDFFASAPAGGDLYLLKSIIHDWDDERCAAILRHIRDVVPD
                     HGSLLIVEPVLPATVPPDQQDNVYLSDLNMLVNVGGRERTADDFAALCTAGGFTLRSVT
                     PLPAPNIFQIVEASPV"
     gene            9510..10184
                     /locus_tag="MWG59_RS00335"
                     /old_locus_tag="MWG59_00335"
     CDS             9510..10184
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971207.1"
                     /locus_tag="MWG59_RS00335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MWG59_00335"
                     /product="response regulator transcription factor"
                     /protein_id="WP_248583836.1"
                     /transl_table=11
                     /translation="MRVLLVEDDDDLRDVIASGLRDGGFAVDCASDWPEADELLHVTAY
                     DCVVLDRMVPSGDTLGPLESRRRAGWSVPVLCLTALDSLHERLRGLESGADDYLAKPFS
                     MRELVLRVRGLSRRAPARLPSFLSCADVVMDVARHEVRRGGVLLSLSPKEYAVLQQLLV
                     HRDAVVTRTGLLEHCWDEMADPVSNVVDAVVAGLRRKLGSPGLVHTVRGQGFLLSAEPG
                     PS"
ORIGIN
        1 gccaccacga gggcgtcggc cagccggtcc cggtaggagg gcgcggccgg atcgggcagc
       61 aggacttcgc aggcggtgag gagcagcacg cccgccgtca ggcccccgag ccgctcgacg
      121 agcgtgccgg gggcgtacgg cggaaagcag gcgaggatgt agaacagctg gaggccgggg
      181 gcgatgcccg cggggcgggg gccggcgacg gcggcgaacg ccagcaggaa tccgacgagg
      241 agcatgccgc cgacggcggc cggggtgtcg gccgccagca gggttcccag ggtcgcgagg
      301 accagtgccg ggggcagggc ccgcagcatg gtggaagccc gttggcggcc ggagccgggc
      361 acggcggaga gcagacccat ggcgatgggc gcgaagagcg cgtacagcgc cgcgacgggg
      421 agtccggccc cgtacagcag cggatagaac ccggcgctcg ccgcgccggc gacgcggacg
      481 gcccggcgca ggatcgtcgc ccggtcctgc gcggcggtca cttccccgct ccgctcatcc
      541 ggcgctcctc tcccggccgg gccgcggtgg cgtacctccg tccggctccc gccattgtcg
      601 gacgcctacg aaccgcccgc gcgcgctgga ccggagaacg cggcccggag agcggcgcgg
      661 gccgccatgc ctcggcctgg gcggcgtcga ggatcaggcc cgcgacggag cagggaggcc
      721 ggtgggttcg ccgggcctgt cccacccatg tccaccacag cccgcggagc cgcgtccggg
      781 cgacggctca tccctccgct ccgggcggag atccgtccgg ggacggtcct gcgcgcctcg
      841 gccgacccca tcgggccgcg cagcgcccat gagccggcgg acgggcacgt cgacatggcc
      901 tggaaggggc gctccggtcg gcgggaaccc cggacccgga gcatgggcga gcgtccgccc
      961 cgagcacctc atcaggcgct cggggcggac gcttgcgtgg tgggggcggc cgggcggccg
     1021 acgcctcaga tcgtcgccgt gtcgatcacg aagcggtagc ggacgtcgct ggcgagaatc
     1081 cgctcgtacg cctcgttgac ctggcccgcg tcgatcacct cgatctcggc gccgagtccg
     1141 tgcacggcgc agaagtcgag catctcctgg gtctccttga tcccgccgat cgccgaaccc
     1201 gcgatcgact tgttgccgag gatcagggag aagaggttca gcgagatcgg ctcctcgggg
     1261 gcgcccacat tgaccagagt gccgtcggtc cgcaggagac cgaggtaggc gccgaagtcc
     1321 agcggggccg agaccgtgga gaggatgacg tcgaaggtgc cggccagttc ctcgaacgtc
     1381 cgggggtcgc tggtcgcgta gtagtggtcg gcgcccagct tgagcccgtc gtccttcttg
     1441 cgcagcgact gggagaggac cgtgacctcg gctccgagcg cgtgcgcgat cttgacggcc
     1501 atgtggccga gaccgccgag gccgacgacg gcgaccttct tgccggggcc cgcgccccag
     1561 cgcttgagcg gggagtacgt ggtgatgccc gcacacagga gcggcgcggc ctcgtccagg
     1621 gcgatgccct cgggaatgga gagggcgaaa gtctcggcca cgacgaggtg cgtcgagtag
     1681 ccgccgtagg tgggctcgcc gctgcggtcg agagcgttgt acgtctgggt gttcccctcg
     1741 gcgcagtgct gctcgcggcc catcaggcac gcgtcgcact ttccgcagga gtcgaccatg
     1801 cagccgacgc cgacgcggtc gccgaccttg aaccgggtga ccccggagcc ggtctcggcg
     1861 acgatcccgg cgatctcgtg gccgggcacc atcgggaaga tgccctcgcc ccagccgtcg
     1921 cgggcctggt ggatgtcgga gtggcagatc ccggcgaact tgatgtcgat caggacgtcg
     1981 aactcgccca ccgggcggcg ttcgatcgtg gtgcgctcca gcggggcctt ggcacgggga
     2041 gcggcatagg cggcaacggt ggtcatgccg gaggttctcc tctgggtgat gcggtaggaa
     2101 cgtctcccag catcaccccg cgacggatgt ttacccaggt caccgctttg cctacgtcca
     2161 gcggtcctac tactgacggg gacaggctcc ggcgcgtacg accaggaatc acaggaataa
     2221 tggaagccat ggacgatcag ccagagcccg cccccgagcc gctggaccgg cgcgccgagc
     2281 tcagcgagtt cctgcgcacc cggagggcca agctccggcc gggcgacgtg gggcttccgg
     2341 agttcggccg gcaccgccgg gtgcccggtc tgcgccggga ggagctggca cagctggccg
     2401 gggtgtccgt ggcgtactac acgcgcctgg agcagggcaa cgggcgcaac gtgtcggccg
     2461 aggtcctcga cgcgatcgcg cgggccctgc ggctcacgga cgccgagagc gcgcatctga
     2521 cgcacctggc caagcccgcg cggcacaaga agaagcgccg cccggcccgg gtgcagcggg
     2581 tgcggaaggg gctgctgtat ctgctcgaca gcatggacgg catccccgcg tacgtgaccg
     2641 gggcgcgctc cgacgtgctc gcctggaatg cgatggcggc ggcggtgttc ggcgactggg
     2701 gcgcgctgcc gccggccgag cgcaactggg cgcggctggt gttcctctcc cccgcctacc
     2761 gggacctgtt cgtgaactgg gactccaagg cctcggacat ggtcagctat ctgcggctgt
     2821 acgcgggctg ccaacccgac gatccggaac tgtcggcgct ggtgggcgaa ctctccgtca
     2881 agagcgatga gttccggcgg ctgtgggcca cgcacaacgt caaggagaag ggccacggcg
     2941 tcaagctgct gcggcatccg ctcgtcgggg agctgaccct gtcgtacgag acgctgaacc
     3001 tcccggacga cgaggagcag cacctggtga cctaccacgc ggagccgggc tcggagtcgg
     3061 cccagtcctt gcgcctgctg gcgagctggg gtgccgacgc gggattgacg gacgtcatag
     3121 gcgacgcggg gcggggcccg gccgacgagc gatgaggacg agctcccggc ctggccggtc
     3181 gggtgcgccg cgcacctcgc gcacgacgaa cccctgtgcc gccaggtccg cctcggcctc
     3241 ctgacgttca cggaagcgga gggtggagtc ggaggtgagg acctgcccgt cgcgccggaa
     3301 cacgtaggtc cagcggaacg acacgagcgg cccgacgatc tcggtcacct cgacccaggt
     3361 ctcgacgggg ccgacgtcgg ggatctcggt gacgcggtgg gaactctccc ggttccatgt
     3421 ctcccaggcg cgccgggccg gatccctggt ctcgaagacc agctgcccgc cgggccgcag
     3481 cgcttcgtgg gcgccgtgca gtgtcgcctc ccagtcgctc gggtcgacga tctcctgcgc
     3541 tgcgttggcc gtcagggtga ccaggtcgac ccgcaacggc ggaagggccg cggcgtcacc
     3601 gtggatccaa cggacccggt caccgcccgg cttggcgcgc gccacgtcga gcgaggcccg
     3661 agcagggtcg accccgacga cctcgatccc acggtccgcc agcatcaggg cgaacacgcc
     3721 cgtaccgcag ccgatgtcca gcacccgccg cgcgccggac tcctcggcga ggcggacgta
     3781 cggttccagg tcgccccggt cgggatcgag tgggtcgtag agggcagcca gccggggatg
     3841 caggaaattc tcgtcagcca tggggcggaa ggtacagggt cctgtcgctg gagcaacgtg
     3901 cggaactgga cccgcgtacc cgcgtacccg ggagatcggg agatcgggag attgtccgac
     3961 cgactcaccg gtttcccttt cggataaacg gaggaatgcg gctgaggctc ccgggccctt
     4021 ttttcgggcc cgcctatcct gagaaccgtg aaagcggggc ggaaaaggaa tacgcgcgat
     4081 gccgcacgta tccgctgccc gcaaggagcc gatgcgtcct actcgcccgc gaaacggccg
     4141 gcttccggcg cgaacgcctc cgcgtgctcg gccgaccaga tgcggtacgg cctggccgga
     4201 cggcccgtca ggtcctggat cgtgggcacc acggccgcct tcgcgcccgc ccgctgccgc
     4261 tccgcgctct gcaggaaggc ctcagcgacg ggcggcggat acttcgccag cagggccgtg
     4321 cgcgcggcgt ccacgcccag ttcctcgaag cgcaacggcc gctccagcac ccgggagagc
     4381 tgagccgtct gctcccgcgc ggtgatcgcc tccgggcccg acacggcgta cgccctgccc
     4441 tcgtgtcccg taccggtgag aacggcgacg gccaccgcgg cgacgtcacg tgggtcgacg
     4501 cacgcgaccg gggagtcgcc gtacagggcg cgcaccactc ccgtcgaccg gatgccgggc
     4561 gcccaggaca gcgtgttgga catgaaggtc ctggggcgga cgaacgtcca cgggaccccc
     4621 gattccctga ccgcctgttc gttctcccgc tggcgccggg tgatgaagtc gtcggcgccc
     4681 ggctcctcca ccgccatcat ggacagcttg accaggtggc gtacgcctgc cgcggccgcc
     4741 gccgcggcga cccgctcgtc gtcgggttcc gtggggctgt tcgtcaccag gaacaccgag
     4801 gcgacgcccc gcagggcccg gtcgagagcg gcgcggtccc cgtactcccc ctcgacgacc
     4861 tcgacccctt ctccccgtac cgtgagccgc tcgggacggc gcgcgagaat ccgcacgggg
     4921 ccggccgccg ccagctgtcc ggcgacctca cggcccaccg ctccggtcgc acccgtcacg
     4981 agaatcactc gggaaacctc tcagccgtcg gcccgggggc cggacaggat ggtggagaac
     5041 accgcttcac cgtcctgatg gcctgtcacc tggaccgtca ccgtatcctc ggccgtctcc
     5101 ggcaggacca ccgcccctat ccagcacggg ctgccgaact cggagtaccg gtggaaccgg
     5161 gtgcgcgcct ccgccggcac gattccggcg ggccccaccg ccagccacgc cgcctggcgg
     5221 gccgcctcca gcagcaacat gcccgggacg tggtcgttgg ggcgctggaa cagggtggga
     5281 tgccgggtgt ccacacgcag ctcccagact ccgtcccgcc cggtctccga gagcaccacg
     5341 tcctcggcgc gggcacggcc cgccaggacg ggcgaaaccg gcgcggtctg cggtatggat
     5401 atcccctcgg ccggggcctc gccgcgcatc cgcctgtacg ccttgggggt gctgaaccgg
     5461 gtctccccca tccccgtcgc ggcgagccgg ccgtcccggt gcaccgccca gccgacacgc
     5521 ccctgcgccg ggagcccgcc ccgccacttc aggtcggagc agaacacctc cacgtcgatc
     5581 tcggtggggg cgccccccac ggtcaggtgc tcctgatggc agacgtagtc cagtcccgac
     5641 atcaggaact ggtagccgac cgggatcccg taaccggcgt ggaaggcgag catggccgcc
     5701 tgccgcatcg cctccgcgac cagcagcggg tcgtgccggt cgtcacccac cggggcgaag
     5761 aacggatggt cgtgcgggag aaccgccccc accgagaacc ggtcgtgccc caggcgtacc
     5821 cagttccgcg gaaaggcgtc ctccggcctg gtccggtgca ccatgtcgat ctccacggga
     5881 tgcaccacct cggcctccgc gtccatggtc ggcaccggat cgatcaagac tgctgcttcg
     5941 ggcataactc cccccagggg tcatgtagtg gcgagccgtt cctgcgatgc acataccggc
     6001 aagattaggt acagactaat aggttttctt ctccggccag ggtgagaccc cccacaccga
     6061 cccggccgat tcgccggccc cgctcgggaa tccggatgac agcggcgcgg gcgtccggta
     6121 aatgcgtccg ccaatccgcg agaatcaccg gaattcccaa aagcgccaac tgcgcgaaaa
     6181 gcgatatccg ctgccgtttc acatgcggcc acgggcgccc ggcccgggag accgggcacg
     6241 aacagatgcg acccgtccag ggcgagaacg acggcccgtt gcacgcttcc catggcacga
     6301 tcctgtcgga agatggcgat catggcatgg tacgccgggc gagcaccctc gaccctggac
     6361 gcctgaagcg cccagggagg tccctgggcg caggcggtca ggcggtcagg cggtcaggcg
     6421 gtcaggcggt caggcggtca ggcggtcagg cggtcaggcg gtcaggcggt caggcggtca
     6481 gagccagttt cgcgccgagg ccgatcatca tcacggcgac caggccgtcc agcacccgcc
     6541 acgcggaagg ccgcgcgagg actccgctca gcaaccgggc gccgaacccg agcgcggcga
     6601 accagcacac gctggcgaga acggcgccga ggccgaaggt ccagcgcaac ggccctcggt
     6661 cggcggcgac ggacccgagc aggaacacgg tgtcgaggta cacgtgcggg ttgagccagg
     6721 tcatcgccag gcaggtcagc accacgcccc ggcgtgaggc ggccggggcg tcccccgcgt
     6781 gcagggccga cgggcgcatg accctgcgcg cggcgagcag gccgtacccg atcaggaaca
     6841 cgccgccgag cagccccacc accgtcagcg ccgacggcca ggcgacgacg agagcgccga
     6901 ccccggcgac ccccagcgcg atgagcaccg catcggacag ggcgcagatc ccgaccaccg
     6961 cgagcacgcc gtcacggcgg acgccctggc gcagcaccag ggcgttctgg gctccgatgg
     7021 cgacgatgag cgagaggccg gtgccgaaac cggcggccgc ggtggtcagg gcgttcgtca
     7081 tgcgctcgac gctacggagc ggaagaacat ggtgtccagc taaagattct tacgtatcat
     7141 taacagtcat gatgtccgag ctccctctcg accaggtgcg gaccctgctg gccgtggtcg
     7201 acgaaggcac gttcgactcg gcggcgaacg ctctgcggct gacgccgtcg gcggtcagcc
     7261 agcgggtcaa ggcactggag cagcgcacgg gccgggtgct gctgatgcgc acgaagccgg
     7321 tgcggcccac cgagtccggt gaggtggtgg tgcgcttcgc ccgccagctg gccaggctgg
     7381 agcacgacgc gcagacggcg ctcggaatgt ccggtcccgg cgagccgacc atcctgccga
     7441 tcgcggtgaa ctccgattcc ctggcgacct ggttcctgcc cgccctgcgg cgcgtgccgg
     7501 aggagttggg gctctgttac gagctgcgcc gggaggacca ggaccatacg gccgccttgc
     7561 tgcgggaggg actggcgatg gccgcggtga cctcctcgcc ggaggcggtg acgggctgct
     7621 cggtgcgccg gctgggccgg atgcggtatt tgccggtggc cagtcccggt ttcgccgacc
     7681 ggtggctggg acggcgggaa ggcaccgcgt tgcgcgagct gatcgccgac gccccggtga
     7741 tcggcttcga ccggcgcgac gaccttcagg acgccttcgt gcgccgactc ggccccggac
     7801 ctcgggcgag cgcgcggcgc cacctggttc ccacgtcgga ggggttcgcg aacgccgtgg
     7861 cgacgggcat gggctggggc atggtgcccg aggttcaggc ggagccgttg ctgagcagcg
     7921 gccggctggt gcggctcacc ccggaccaca ccgtggacgt gccgctgtac tggcagcagt
     7981 ggaagctgga atcaccggcc ctggcggccg tctccgaggc ggtggcggcg gaggccgccg
     8041 aagcgctcga cggcgactgg cccgactgag cggcacacac cgcccgccac cggaaccccg
     8101 ctcgccacgc ctatgggtga ttgccacccc acccctccaa ggtcaggttt acctaactaa
     8161 acagaaaggg gcaggcgatg acggaccgtc acggccagga ggcgggcaac ggccgggagc
     8221 agcccatgga ttgggcggca cggggggcgg tggcccggct cgtgctcggc cagatggcca
     8281 cccaggcgct gggcacggcg gtgcggttgg gcgtcttcga ccggatcggg ccggacgagc
     8341 tgagcgccga ggcgctcgcc ggttccctcg gcacccaccc gcaggcgacg ctgcgtctgc
     8401 tgcgcgccct ggccggactc cagctgctgt ccgagacgga accgagcgcc ttccggacca
     8461 ccacggccgg cgacgccctg cgcgccgaca cacccggcac gatggtcgcc atggcccgga
     8521 tgttcaccga cccggtcatg ctgcggggct gggacctgct cgacgagagc gtccgtacgg
     8581 gagagaccac gttcgacacg gtgttcggga ccgacttctt cggccacctc aaggaacacc
     8641 ccgagctgtc cgcctcgttc aacgaggcga tgagccaggg cacacggctg accgccacga
     8701 ccgtcccgca ccactacgac ttcgggcgct tccacaccct cgtggacatc ggcggcggcg
     8761 acggcacgct gctggcctcg gtccttcgcg agcacccgaa actgcggggc gtcctgttcg
     8821 acaccgccga ggggctcgcg caggctccta gggcgtgttt cgaaagtagc gccgtccgcc
     8881 cggagggcgg gctcggcggc gtctggtgcg tgcgatcgca aggcggaggg tcaccccgat
     8941 actggacgta tcggggtgat cccgacaacg cggcgagcgt gcgtgccagg cgtcgccgag
     9001 caggcgggac tttcgaaaca cgccctagac ggctggccca ggagggtctg accgagcgcg
     9061 tcgccctgga gaccggcgac ttcttcgcct cggccccggc cggcggcgac ctctatctgc
     9121 tcaagagcat catccacgac tgggacgacg agaggtgcgc ggccatcctg cggcacatcc
     9181 gcgacgtcgt ccccgaccac ggctccctgc tgatcgtcga accggtgctg cccgccaccg
     9241 tgccgccgga ccagcaggac aacgtctacc tcagcgacct caacatgctc gtcaacgtcg
     9301 gcggccggga gcggaccgcg gacgacttcg cggccctgtg cacggcgggc ggcttcaccc
     9361 tccggagcgt gacaccactg cccgcgccca acatcttcca gatcgtcgag gcctccccgg
     9421 tctgacgggc cccccggggg cgggtgcccg gctcgcctta gaatgacgcg tccctccccc
     9481 ccggaagaag actcggaggc atcctcccca tgcgtgtgct gctggtggag gacgacgacg
     9541 acctgcggga cgtgatcgcg tccgggctgc gggacggcgg cttcgccgtg gactgcgcgt
     9601 cggactggcc ggaggcggac gagctgctgc acgtcaccgc gtacgactgt gtggtgctgg
     9661 accggatggt gccctccggc gacaccctcg ggccgctgga gagcaggcgt cgggccggct
     9721 ggtcggtgcc cgtgctgtgc ctcaccgctc tggactccct gcacgaacgg ctgcgggggc
     9781 tggagagcgg ggcggacgac tacctggcca agccgttctc gatgcgcgag ctcgtcctgc
     9841 gcgtccgggg cctgagccgt cgggcgccgg cccggctccc ttccttcctg agctgtgcgg
     9901 acgtggtcat ggacgtggcg cggcacgagg tgcggcgcgg tggtgtgctg ctgtcgctga
     9961 gcccgaagga gtacgccgtg ctccagcagc tcctcgtcca ccgggacgcc gtcgtcaccc
    10021 gcaccgggct gctggagcac tgctgggacg agatggccga cccggtctcc aatgtggtgg
    10081 acgccgtcgt cgcgggcctg cggcgcaagc tcggctcccc cggcctggtg cacaccgtgc
    10141 gggggcaggg gttcctgctg tcggcggagc cggggccgtc ctgatggcga agcccctgcg
    10201 gcctcgccgc tccccggcca ggagacggct gcaccggctg cggctgcggc tgaccgccgc
    10261 gtacaccctg atcaccgtcg tcgggctggc ctgtctgtcc tggctggtga tccgtaccga
    10321 cgaccgggcg cggctggacg ccgagtacga cgagatgcgc cgccgggcgt ccgtcgcggc
    10381 ctcgctcgtc tattacgagg acggccggat ccgtctggac gggctcgacg acgacgaggc
    10441 gacctcgggg actccgcagg tcctggtgct ggagggccgg cccggcggtg atccggtcat
    10501 cgtgttccgg ggccgcaccg cgcagttcgc cgtgccggcc ggggcgctcc gggctgcggc
    10561 tctcgctgcg atggacgacg aagggccggt gcgggccgag gcacgcgacc gcgcgggaga
    10621 gcccgttcac gtgctggccg tgcccttcta ccacgacgcc accgacgagg tggcgggcgc
    10681 ggccgtcgcg gtcggcgatc cggagcgcgg ggcggccgag caccgcagtc tggtcctctc
    10741 cctcgtcgtc ggggccggtg cgctgacggc gctggccgcg ctgaccgggc acgtcctctc
    10801 cgcgcggagc atgcggccgg cctgggaggc gctggagcag caggagcggc tgctggccga
    10861 cgcggcgcac gagttgcgga ccccggtcgc ggtgatgcgc ggatcggtgg aggtggccgc
    10921 cggtgctccg ggcgggctgg aggct
//
