LOCUS       NZ_CP051627            10405 bp    DNA     circular CON 21-APR-2024
DEFINITION  Thermobifida alba strain DSM 43795 chromosome, complete genome.
ACCESSION   NZ_CP051627
VERSION     NZ_CP051627.1
KEYWORDS    RefSeq.
SOURCE      Thermobifida alba
  ORGANISM  Thermobifida alba
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsaceae; Thermobifida.
REFERENCE   1  (bases 1 to 4903256)
  AUTHORS   Luzics,S., Horvath,B., Nagy,I., Toth,A., Nagy,I. and Kukolya,J.
  TITLE     Thermobifida alba genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4903256)
  AUTHORS   Luzics,S., Horvath,B., Nagy,I., Toth,A., Nagy,I. and Kukolya,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2020) NGS Platform, Seqomics Ltd., Vallakozok u.
            7., Morahalom 6782, Hungary
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: RA v. v0.2.1; CLC Genomics Workbench v. 11.0.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 331.0x
            Sequencing Technology             :: PacBio Sequel; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023208015.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 13:45:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,356
            CDSs (total)                      :: 4,290
            Genes (coding)                    :: 4,246
            CDSs (with protein)               :: 4,246
            Genes (RNA)                       :: 66
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 10 of 44
            Pseudo Genes (incomplete)         :: 35 of 44
            Pseudo Genes (internal stop)      :: 3 of 44
            Pseudo Genes (multiple problems)  :: 4 of 44
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:37:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4317075
            Orig. end                         :: 4327480
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP051627.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="13"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            338..1102
                     /locus_tag="FOF52_RS19285"
                     /old_locus_tag="FOF52_19165"
     CDS             338..1102
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290744.1"
                     /locus_tag="FOF52_RS19285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FOF52_19165"
                     /product="protein phosphatase 2C domain-containing protein"
                     /protein_id="WP_068753613.1"
                     /transl_table=11
                     /translation="MGVARGAVEVTVLTHRGAVRQANEDAVVMGALTVAGAEMNAPAHC
                     WLPIADPVIIAVADGLGGHAAGEIASEHAVHRMAESGPQLTGPESVDLLLKHIDDEIKE
                     HAGQHVEFSGMGTTVAGVLLTPDANYWFNVGDSRTYRLKDSRLIQLSEDDSPVLPPAED
                     GRPVTTNYITQSLGGSGNTAMVPHVGQDTEPGPGAWLMCSDGLSDLVPLEEMERIIAEA
                     GDDNIAVYNLWKAAMNASGRDNISILLVRRHS"
     gene            complement(1167..1790)
                     /locus_tag="FOF52_RS19290"
                     /old_locus_tag="FOF52_19170"
     CDS             complement(1167..1790)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290745.1"
                     /locus_tag="FOF52_RS19290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FOF52_19170"
                     /product="copper resistance CopC family protein"
                     /protein_id="WP_248591317.1"
                     /transl_table=11
                     /translation="MPATSASSPGGRITALLTGLLLALALSVGLAPAAQAHNTLVSSSP
                     EDGATLDAPPEEVVLTFNANVMEGGNGIVVTGPDGTDHADGEVVIDGVTASVGLTPLTQ
                     PGEYTVEYRIISADGHPLSDSLSFTLDESAVPEPAESESPSPAEQPGEDDTSSPVSDEE
                     EAATDARSLAGPLAPVLGVLAAIGGIAVIAIIVIRLRRRPGSGD"
     gene            complement(1998..2264)
                     /locus_tag="FOF52_RS19295"
                     /old_locus_tag="FOF52_19175"
     CDS             complement(1998..2264)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017538765.1"
                     /locus_tag="FOF52_RS19295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FOF52_19175"
                     /product="WhiB family transcriptional regulator"
                     /protein_id="WP_248591318.1"
                     /transl_table=11
                     /translation="MNRLYHESEDWVRQGACRNYDPELFFPVSTTGPGRETVELAKAIC
                     RSCTVRQECLRWALRAGEAHGVWGATTPEERRYLRRELLPAAS"
     misc_feature    complement(2618..2633)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.13"
                     /tool="antismash"
     misc_feature    complement(2846..2863)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.63"
                     /tool="antismash"
     misc_feature    complement(2889..2907)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 22.32"
                     /tool="antismash"
     gene            2985..3491
                     /gene="ectA"
                     /locus_tag="FOF52_RS19300"
                     /old_locus_tag="FOF52_19180"
     CDS             2985..3491
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546867.1"
                     /locus_tag="FOF52_RS19300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FOF52_19180"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_282573721.1"
                     /transl_table=11
                     /translation="MAAETGDVLLEPPSLDDGRRLWQLARDSGLDVNSPYAYVLWCRDF
                     AATSLVARDSAGTVRGFVTGYVRPEAPDTYFLWQVAVDSAFRGRRLARRMLDRVGARIA
                     ERGIRYLEATVTPDNTASRALFTSFARARSADLSWSPLFDRGHFPAENSEQHEREDLVR
                     IGPLS"
     gene            3567..4832
                     /gene="ectB"
                     /locus_tag="FOF52_RS19305"
                     /old_locus_tag="FOF52_19185"
     CDS             3567..4832
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (22-354). E-value: 3.1e-71.
                     Score: 232.1. Matches aSDomain:
                     nrpspksdomains_FOF52_RS19305_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610153.1"
                     /locus_tag="FOF52_RS19305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FOF52_19185"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_248591319.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.4e-71, bitscore:
                     239.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFSHLESEVRSYCRGWPTLFDKARGSRVYDTSGRDYLDFFAGA
                     GSLNYGHNNPALKSALLDYLGSDSVVHSLDAYTVAKRDFLSTFEEVILRPRGLDYKVQF
                     PGPAGNNAVEAALKLARKVTGRETVISFTNSFHGMTLGALAVTGNSMKRGGAGVPLSHT
                     ATMPFDNYMDGQVPDFLWLRSLLEDSGSGLDRPAAVIVETVQGEGGINVASAKWLRGLA
                     ELCQEYELLLIVDDIQMGCGRTGPFFSFEEAGIVPDIVTLSKSISGYGLPLALTLFKRE
                     LDVWEPGEHNGTFRGFNPAFLTATVALDTYWRDEVFQKETLAKGDLIAERLDAIADEHA
                     EAGATTRGRGMARGLVLPGEGDAKRVCAEAFERGLLMETSGPEDEVAKLLPPLTTSVTE
                     LEEGLDILAESVRAAIRTPQPA"
     aSDomain        3633..4628
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FOF52_RS19305_Aminotran_3.1"
                     /evalue="3.10E-71"
                     /label="FOF52_RS19305_Aminotran_3.1"
                     /locus_tag="FOF52_RS19305"
                     /protein_end="354"
                     /protein_start="22"
                     /score="232.1"
                     /tool="antismash"
                     /translation="DKARGSRVYDTSGRDYLDFFAGAGSLNYGHNNPALKSALLDYLGS
                     DSVVHSLDAYTVAKRDFLSTFEEVILRPRGLDYKVQFPGPAGNNAVEAALKLARKVTGR
                     ETVISFTNSFHGMTLGALAVTGNSMKRGGAGVPLSHTATMPFDNYMDGQVPDFLWLRSL
                     LEDSGSGLDRPAAVIVETVQGEGGINVASAKWLRGLAELCQEYELLLIVDDIQMGCGRT
                     GPFFSFEEAGIVPDIVTLSKSISGYGLPLALTLFKRELDVWEPGEHNGTFRGFNPAFLT
                     ATVALDTYWRDEVFQKETLAKGDLIAERLDAIADEHAEAGATTRGRGMARG"
     misc_feature    4908..4921
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: strong, score: 19.09"
                     /tool="antismash"
     misc_feature    complement(4924..4937)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.88"
                     /tool="antismash"
     misc_feature    complement(4998..5011)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.58"
                     /tool="antismash"
     gene            5001..5405
                     /locus_tag="FOF52_RS19310"
                     /old_locus_tag="FOF52_19190"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290749.1"
                     /locus_tag="FOF52_RS19310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FOF52_19190"
                     /product="ectoine synthase"
                     /protein_id="WP_248591320.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.4e-53, bitscore:
                     177.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLDDVNGTDADIVTENWRSRRIVLARDGVGFSFHETVLYAG
                     TETSMWYANHIELVHCIEGEAEVTNDETGETFLIKPGMLYLLDGHEHHTVRPKTDFRVL
                     CVFNPPVTGREVHDENGAYPLLTEEAVAAD"
     gene            5467..6375
                     /gene="thpD"
                     /locus_tag="FOF52_RS19315"
                     /old_locus_tag="FOF52_19195"
     CDS             5467..6375
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017621331.1"
                     /locus_tag="FOF52_RS19315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FOF52_19195"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_248591321.1"
                     /sec_met_domain="PhyH (E-value: 3.9e-38, bitscore: 129.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTALTNEAARQTADDYPTRKASEPALLYRKHPVVWGDREGPIDSE
                     TLDSYEAKGYLQVEQLLTADELAACRAELDRLSSDEELKRDERVVVERTSNEVRSVFDV
                     HKISPLFADLVRDPRVVGRARQILGSDVYVHQSRINYKPGFGGGDFYWHSDFETWHAED
                     GMPQPRATSISIALTENYVHNGALMIMPGSHKTFVSCVGRTPEDHYRESLKKQEIGVPD
                     QNSLTILAEKHGIDVLTGPAGSAIMFDCNCMHGSGGNITPFPRSNIFIVFNSVENTCGA
                     PFSAPRPRPAFLGARDFTPVM"
     gene            6558..7448
                     /gene="ehuB"
                     /locus_tag="FOF52_RS19320"
                     /old_locus_tag="FOF52_19200"
     CDS             6558..7448
                     /GO_component="GO:0009897 - external side of plasma
                     membrane [Evidence IEA]"
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033294 - ectoine binding [Evidence IEA]"
                     /GO_function="GO:0033295 - hydroxyectoine binding [Evidence
                     IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188113.1"
                     /locus_tag="FOF52_RS19320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FOF52_19200"
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="WP_248591322.1"
                     /transl_table=11
                     /translation="MTDHKNLDRRGLFRAGGIGLAAVALGPALAACSRTEDTVTLDSLR
                     EQGYIRVAINNEAPFGYIGEDGEVTGSSPELLRAIFAELGVDDVRAESVAWDGLIPGLK
                     AKRFDAVAAGMYVTPERCAEVAFAEPDYRILQAFLVPSGNPHGLSTFDDIAADPDVTVA
                     VLNASVEQGYAEGAGVPADQIETVDNQVSAYELLQKGRVDAVALSTISLNRLLEQRGGD
                     FEVTEGFIPVVDGEEVTPAGALAFRKSDTALLDEVNRVLAEFKENGRLLEIIEPFGFTE
                     QELPGDLTIEQLCQA"
     gene            7463..8188
                     /gene="ehuC"
                     /locus_tag="FOF52_RS19325"
                     /old_locus_tag="FOF52_19205"
     CDS             7463..8188
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuC"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156203.1"
                     /locus_tag="FOF52_RS19325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FOF52_19205"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="WP_248591323.1"
                     /transl_table=11
                     /translation="MDFFIDRLPLYLSGAGTTLVITLLGAALAFVLAMVVGLLGTLRNL
                     AARAVATCYTETFRGVAALVLMYWMAFAIPQLTPYEIDPVFAAVLALGLNVGAYGAEVV
                     RAAVNAVPPTQIEATVALNMSWWRRTWAVVLPQAWAQMLPTFGNLVIELMKATAIVSLI
                     GVADLTRIAQEQRAATGDTIAAFAGALVVYFVLAQLLIIGMRLLERRANRRLGRLPASD
                     SGLWSLLRPPPVTAAGGGR"
     gene            8188..8826
                     /gene="ehuD"
                     /locus_tag="FOF52_RS19330"
                     /old_locus_tag="FOF52_19210"
     CDS             8188..8826
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuD"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017538772.1"
                     /locus_tag="FOF52_RS19330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FOF52_19210"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="WP_248591324.1"
                     /transl_table=11
                     /translation="MTWDWEFTGKIVPDLLDGLVVTVQATLLGYVLALVLGLAVALARR
                     VPVLGTAVFAVMEFIRCTPLLVQLVFVFYLAPSYVSAFTVGVAVLGVHYAAYTAEVYRS
                     GIEGVPPGQWEACRALSLPAHRTWGAVVLPQAVRKVIPALGNYLIAMFKDTPLLFAITV
                     GELMYQASRIGGVHFRYLEAMTLVGLFFLLVSVPSAIIIRQLERRYAAV"
     gene            8813..9622
                     /gene="ehuA"
                     /locus_tag="FOF52_RS19335"
                     /old_locus_tag="FOF52_19215"
     CDS             8813..9622
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290753.1"
                     /locus_tag="FOF52_RS19335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FOF52_19215"
                     /product="ectoine/hydroxyectoine ABC transporter
                     ATP-binding protein EhuA"
                     /protein_id="WP_248591325.1"
                     /transl_table=11
                     /translation="MPQSEPSPGTDQHEQEPLIRFDGVVKRFGANTVLDHLDFTVAPGE
                     RVTLIGPSGSGKTTILRLLMTLEQVTNGVIWVGGEPLSHMHRGGRLVRANEQHQRRVRR
                     QIGMVFQQFNLFPNMTVRQNITEGPVHSLGVPRVEADRRAAELLEMVDLADKIDAHPTQ
                     LSGGQQQRVAIARALAMSPRILLLDEVTSALDPELVTGVLDVLRTVAETTDITMLLVTH
                     EMQFARDVSHRVLMFDQGRIVEEGPPEQIFGDPRQERTRAFLHAVLD"
     regulatory      9778..9841
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /regulatory_class="riboswitch"
     gene            9895..10095
                     /locus_tag="FOF52_RS19340"
                     /old_locus_tag="FOF52_19220"
     CDS             9895..10095
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290754.1"
                     /locus_tag="FOF52_RS19340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FOF52_19220"
                     /product="CbtB-domain containing protein"
                     /protein_id="WP_068753598.1"
                     /transl_table=11
                     /translation="MSAVHAPRVGAELGPVPLALTATAVTVLALLVLYLVGFDQGAVSR
                     TGMYLHELMHDGRHLMGVPCH"
ORIGIN
        1 cccgccgggc atcacactcc ccgtagggag tcgggggccc ggagcggtcc ggtggttccc
       61 gcgtggagga cggccaggaa ccgtcggccg gatacggcgg taaggaaggt ggcggtcgtc
      121 tcgtacggcg gtggcgcacc gccgggaggc gatctcggag gtgaaggcgc cggggactgc
      181 gcaccagcac gatttccgcc atgatggaca cggattttga ccacggccga ccgtccatcg
      241 gtacggttac atgatcaatg cgcctcggtt gccgtccagc tggggcgccg cttggcactg
      301 ttcggctgca atgccgacga aacaggaggt agggcgcatg ggtgtcgctc gtggggcagt
      361 cgaggtcacg gtcctcacgc acagaggcgc tgtccgccag gccaatgagg acgccgtggt
      421 catgggcgcg ctcacggtcg cgggcgccga gatgaacgca ccggcacact gctggctgcc
      481 catcgctgat cccgtcatca tcgcggtcgc cgacgggttg ggaggacacg ccgccggtga
      541 gatcgcctcg gagcacgcgg tccaccggat ggccgagagc ggcccccagc tgaccggtcc
      601 ggagtcggtg gacctgctcc tcaagcacat cgacgacgag atcaaggagc acgccgggca
      661 gcacgtggag ttctcgggca tggggaccac ggtcgcgggg gtgctgctga cacccgacgc
      721 gaactactgg ttcaacgtcg gcgactcccg gacgtaccgg ttgaaggact cccggctgat
      781 ccagctgtcc gaggacgact ccccggtgct gccgcccgcg gaggacggcc gtccggtcac
      841 gacgaactac atcacccagt cgctgggcgg cagcggcaac accgccatgg tgccgcacgt
      901 gggccaggac accgagccgg ggcccggtgc ctggctgatg tgcagtgacg gactgtccga
      961 cctggtaccg ctggaggaga tggagcgcat catcgcggag gcgggcgacg acaacatcgc
     1021 cgtctacaac ctgtggaagg ccgcaatgaa cgcgtccggg cgcgacaaca tcagcatcct
     1081 gctggtgcgc cgccactcct gacctccggt cccgcggtac cggtggtacc ccctccggtg
     1141 ggggcaggga gggggcgttc gcggcgtcag tccccggaac cggggcggcg gcgcaggcgg
     1201 atgacgatga tggcgatcac cgcgatgccg ccgatggccg cgaggacacc caggaccggg
     1261 gcgagagggc cggcgaggga gcgggcgtcg gtcgccgctt cctcttcgtc cgagacgggg
     1321 gacgatgtgt cgtcctcgcc cggctgttcg gccggggacg ggctttcgct ctcggcgggt
     1381 tcggggacgg cggactcgtc cagggtgaag ctcagggagt ccgagagcgg gtggccgtcg
     1441 gcggagatga tccggtactc gacggtgtac tcgccgggct gggtcagcgg ggtcaggccg
     1501 acggaggcgg tcaccccgtc gatgacgacc tctccgtcag cgtggtcggt cccgtccggg
     1561 ccggtcacca cgattccgtt gccgccttcc atgacgttgg cgttgaaggt cagcaccacc
     1621 tcttccgggg gggcgtcgag cgtggctccg tcctcggggg acgaggagac cagggtgttg
     1681 tgcgcttgcg cggcgggggc gaggcccacc gacagcgcca gggcgagcag gagacctgtc
     1741 agcagcgcgg tgatccgacc gccgggggac gaagcggaag tagctggcat cgactggacc
     1801 tttccgatgt ctgcgtcgtg atcgggcgga ggacacgtgt ccaccgcagg gcacgcttct
     1861 ccgggaggat gcccgtcacc cccgacgcta ccccccgggc cgccgtcatg ccgttcggga
     1921 agaaccgggc cggtacacgc gtgagccgcc gggcccgacc acccggcggc tcacgcacga
     1981 cctttccgag gctggagtca gcttgccgct ggcagcagct ccctgcgcag atagcggcgc
     2041 tcctcggggg tggtcgcccc ccacaccccg tgtgcctcac cggctcgcag ggcccaccgc
     2101 agacattctt ggcgaacagt gcagctgcgg cagatggcct tggcgagttc cacggtttcg
     2161 cgtccggggc cggtcgtgct caccggaaag aaaagctcgg ggtcgtagtt gcggcacgct
     2221 ccctgccgga cccagtcttc gctctcgtgg taaaggcggt tcaccaggcg cctccgatcc
     2281 ctggctcgtg gctctgactc cccggcggaa ccgtcgggga ccgtggccct gtcgcggctg
     2341 gttctgactc cgtcggccgt ggtgcggctg tcgttgtcgg tgtacggtcg ggcgccccgg
     2401 ccgcggcgac aggtggtagg gcgcctcagc gcctcctgtc gaccgattgt agtactacgt
     2461 tcggtcgtac tacgcaaagt cctagaaaag tcggcccaaa ggtcgtgctg gtgcggccgg
     2521 gagacccctc tcccgcacgg gccgggaagc cctggcaggg cggggaatca gcgcggcggt
     2581 ggcggccgtg attgatcggt cctttgcacc gaattcgtgt gagctaattc acccttaaag
     2641 ttctcttgga tttctcacag gtaagcgtgg ggcccgcact acccgtggga cggcggttca
     2701 caccacgagc ttccggagac ttgggaagac cggcggtatc ccctggtgag ccaccgggca
     2761 gaagaggggg cggcgctcgc tgcgagaggg ggagacggtg gcgcaaagta gcggccccag
     2821 gcaaagggtc ctgtcctacc cggcggcaac accagcgaaa catatgccgg gtctgtcacg
     2881 aaaatcctgc tcagaaacgt tcgctagggt ccttcgacgt gtttgaacaa acgaccccac
     2941 cggtcgaccg acatagcctg gacacagagt gtaaccattc gactgtggcc gccgagaccg
     3001 gcgacgtgct cctggaaccg ccctcgctgg acgacggccg gcgactgtgg cagctcgcca
     3061 gggactcggg cctggacgtg aactccccct acgcctacgt gctgtggtgc cgcgacttcg
     3121 ccgccacatc gctggtggcc cgcgacagcg cgggcaccgt gcgcgggttc gtcaccggat
     3181 acgtgcgacc cgaggcgccc gacacctact tcctctggca ggtcgcggtg gactccgcgt
     3241 tccgcggccg ccgactggcc cgcaggatgc tcgaccgcgt cggagcccgc atcgccgaac
     3301 gcggcatccg ctacctcgaa gccacggtca cccccgacaa cacggcgtcg cgggccctct
     3361 tcacctcctt cgcccgtgcc aggtcagccg acctttcctg gtccccgttg tttgaccgcg
     3421 gacatttccc ggcagagaac tccgagcagc acgaacgcga ggacctcgtc cgcatcggcc
     3481 ccctgagctg acggacccgg acccgcccgg gcggttccga ccgccgcaag cgtgccctga
     3541 aaaccaggag aaaaaggaga tccgtgatgg aaaccttctc ccaccttgag tccgaagtcc
     3601 gcagctactg ccggggatgg ccgaccctct tcgacaaggc gcgcggcagc cgcgtctacg
     3661 acacctccgg ccgcgactac ctcgacttct tcgccggagc cgggtcgctc aactacggcc
     3721 acaacaaccc cgcgctcaag tccgcgctgc tggactacct gggcagcgac tcggtcgtgc
     3781 acagcctgga cgcctacacc gtcgccaaac gcgacttcct gagcaccttc gaggaggtga
     3841 tcctccgacc ccggggcctg gactacaagg tgcagttccc cggcccagcg ggcaacaacg
     3901 cggtcgaggc ggccctcaaa ctcgcccgca aggtcaccgg ccgcgagacg gtcatcagct
     3961 tcaccaacag cttccacggc atgacgctgg gggcgctcgc ggtgaccgga aactcgatga
     4021 agcgcggcgg cgcgggagtg ccgctcagcc acaccgccac catgccgttc gacaactaca
     4081 tggacggcca ggtgcccgac ttcctgtggc tgcgcagcct gctggaggac agcggcagcg
     4141 gactggaccg gcccgccgcg gtcatcgtgg agacggtcca gggcgagggc ggcatcaacg
     4201 tggccagcgc caagtggctg cgcggcctgg ccgagctgtg ccaggagtac gagctgctgc
     4261 tcatcgtcga cgacatccag atgggctgcg gccgcaccgg tcccttcttc agcttcgagg
     4321 aggccgggat cgtgccggac atcgtcaccc tgtccaagtc gatcagcggc tacggcctgc
     4381 cgctggcgct gacgctgttc aagcgggagc tggacgtgtg ggagcccggt gagcacaacg
     4441 gcaccttccg cgggttcaac ccggccttcc tcaccgcgac cgtggcgctc gacacctact
     4501 ggcgggacga ggtcttccag aaggagaccc tggccaaggg agacctgatc gccgagcggc
     4561 tcgacgcgat cgccgacgag cacgccgagg ccggagcgac cacgcgcggc cgcggcatgg
     4621 cgcgcggcct ggtgctcccg ggcgagggcg acgccaagcg ggtgtgcgcc gaggcgttcg
     4681 agcgcggcct gctcatggag acctccggcc ccgaggacga ggtggcgaaa ctgctgccgc
     4741 cgctgaccac ctccgtcacc gaactggagg agggcctgga catcctcgcc gagtcggtgc
     4801 gcgcggcgat ccgcactccc cagcccgcct gacccgtccc cgcgggcagg ccaccgttcc
     4861 cgacggcccc ggtcggggcg cgttcccggc ggtcctgcgc ggtcgcctgg atgcacatgc
     4921 aggtgcatat gcatccaggg cggttgtgcg gggccgcctc ccgccggaag ccgtcaccac
     4981 caccgaaagg aaaccaccgc atgatcgtgc gcagtctgga cgacgtcaac ggcaccgacg
     5041 ccgacatcgt caccgagaac tggcgcagcc gccgcatcgt cctggcccgg gacggcgtcg
     5101 gcttctcgtt ccacgaaacc gtcctgtacg cgggcaccga aacgtcgatg tggtacgcca
     5161 accacatcga actggtgcac tgcatcgagg gtgaggccga ggtcaccaac gacgagaccg
     5221 gcgagacgtt cctcatcaag ccaggcatgc tctacctgct tgacgggcat gagcaccaca
     5281 cggttcggcc caagaccgac ttccgggtgt tgtgcgtgtt caacccgccg gtcaccggac
     5341 gcgaggtgca cgacgagaac ggcgcctacc cgctgctgac tgaggaggcc gtagccgccg
     5401 actgatacgg aatccgtgcg cgtcgaaccg ttcgcagaca agagagaacc cgtgaggggg
     5461 aaccgaatga ctgctctgac caacgaggct gcacgacaga ccgccgacga ctaccccacc
     5521 cgcaaggcga gcgagcccgc cctgctctac cgcaagcacc cggtcgtctg gggagaccgc
     5581 gagggcccca tcgactccga gaccctcgac agctacgagg ccaagggcta cctgcaggtc
     5641 gagcagctgc tcaccgccga cgaactggcc gcctgccggg ccgaactgga ccggctgagc
     5701 tccgacgagg aactcaagcg cgacgagcgc gtcgtggtcg agcgcacctc caacgaggtc
     5761 cgctccgtct tcgacgtgca caagatcagc ccgctcttcg ccgacctggt gcgcgacccg
     5821 cgcgtcgtgg gccgcgcccg ccagatcctc ggctccgacg tctacgtgca ccagagccgc
     5881 atcaactaca agccgggctt cggcggcggc gacttctact ggcactccga cttcgagacc
     5941 tggcacgccg aggacgggat gccgcagccg cgggcgacca gcatctccat cgcgctcacc
     6001 gagaactacg tgcacaacgg cgcgctgatg atcatgcccg gctcgcacaa gacgttcgtc
     6061 tcgtgcgtcg gccggacccc ggaggaccac taccgggagt ccctgaagaa gcaggagatc
     6121 ggcgttcccg accagaacag cctcaccatc ctggccgaga agcacggcat cgacgtgctc
     6181 accggcccgg ctggcagcgc gatcatgttc gactgcaact gcatgcacgg atccggcggc
     6241 aacatcacgc cgttcccgcg gtcgaacatc ttcatcgtgt tcaacagcgt cgagaacacc
     6301 tgcggcgcac cgttctccgc accccgtccg cgccccgcat tcctgggggc gcgtgacttc
     6361 acacccgtga tgtagcggac cggcggcggt gtcccgcacc ccgcgggacg ccgccgcccc
     6421 cgacccgccg acgcggcccc cccggccgga tccccgacaa ccgcccccgc gagggcctac
     6481 cccttcgaac cccccacccc ccacccgagc gttgcggccc cccgtcgccg ccaacgctca
     6541 cggacaggag cgacaccatg accgatcaca agaacctgga ccgccgcggc ctcttccgcg
     6601 ccggaggaat cggactggcc gcggtcgccc tgggccccgc cctggctgcc tgctcccgca
     6661 ccgaggacac cgtgaccctg gacagcttgc gggaacaggg ctacatccgg gtcgcgatca
     6721 acaacgaggc ccccttcggc tacatcggcg aggacggcga ggtgaccggc tcctcccccg
     6781 agctgctccg cgccatcttc gcggaactgg gcgtcgacga cgtccgagcc gagtccgtgg
     6841 cctgggacgg actcatcccc ggactgaagg ccaagcggtt cgacgcggtg gccgccggaa
     6901 tgtacgtcac ccccgagcgg tgcgccgagg tcgcgttcgc cgaacccgac taccgcatcc
     6961 tccaggcgtt cctcgtcccc tccggcaacc cccacggcct gagcaccttc gacgacatcg
     7021 ccgccgaccc cgacgtcacg gtggccgtgc tcaacgcctc cgtggagcag ggctacgccg
     7081 agggcgcggg cgtgcccgcc gaccagatcg agaccgtcga caaccaggtc agcgcctacg
     7141 aactgctgca gaagggccgg gtcgacgcgg tcgcgctgag caccatctcg ctcaaccggc
     7201 tgctggagca gcgcggcggc gacttcgagg tcaccgaagg cttcatcccg gtggtggacg
     7261 gcgaggaggt cacccccgcc ggagccctcg ccttccgcaa gagcgacacc gcactcctcg
     7321 acgaggtcaa ccgggtgctc gccgagttca aggagaacgg ccggctgctg gagatcatcg
     7381 agccgttcgg cttcaccgag caggagctgc ccggcgacct gaccatcgaa cagctctgcc
     7441 aggcctgagg gcggcagccg acgtggactt cttcatcgac cggctcccgc tctacctgag
     7501 cggggccgga accaccctcg tgatcaccct gctcggggcc gcgctcgcct tcgtcctcgc
     7561 catggtcgtg ggcctgctcg ggaccctgcg caacctggcc gcgcgcgccg tcgccacctg
     7621 ttacaccgag acgttccgcg gtgtggccgc gctggtcctg atgtactgga tggcgttcgc
     7681 gatcccccag ctcaccccct acgaaatcga cccggtgttc gccgcggtgc tggcgctcgg
     7741 cctcaacgtc ggcgcctacg gggccgaggt ggtgcgggcc gccgtcaacg cggtgccccc
     7801 gacgcagatc gaagccaccg tcgcgctcaa catgtcgtgg tggcggcgca cgtgggcggt
     7861 ggtgctgccg caggcgtggg cgcagatgct gcccaccttc ggcaacctcg tcatcgaact
     7921 gatgaaggcc accgccatcg tgtcgctgat cggggtcgcc gacctgaccc gcatcgccca
     7981 ggagcagcgg gccgccaccg gagacacgat cgcggccttc gccggggccc tggtcgtcta
     8041 cttcgtgctc gcccagctgc tcatcatcgg catgcggctg ctggaacgcc gggccaaccg
     8101 gaggctgggg cggctccccg cctccgacag cggactgtgg tccctgctgc ggcccccgcc
     8161 cgtcaccgcc gccggaggtg gccgctgatg acctgggact gggagttcac cgggaagatc
     8221 gtccccgacc tgctcgacgg gctggtcgtc accgtccagg ccaccctgct gggctacgtg
     8281 ctggcgctcg tcctgggact ggcggtggcg ctggcgcgcc gcgtgccggt gctcgggacc
     8341 gcggtgttcg cggtcatgga gttcatccgc tgcaccccgc tgctggtgca gctggtcttc
     8401 gtgttctacc tggcgccgag ctacgtctcg gcgttcacgg tcggcgtcgc ggtcctgggc
     8461 gtgcactacg ccgcctacac cgccgaggtg taccgctccg ggatcgaggg cgtgccgccg
     8521 ggacagtggg aggcgtgccg ggcgctgagc ctgcccgcgc accgcacctg gggcgcggtg
     8581 gtgctgccgc aggcggtccg caaggtgatc cccgcactcg gcaactacct gatcgccatg
     8641 ttcaaggaca cgcccctgct gttcgcgatc accgtcggcg aactgatgta ccaggcctcg
     8701 cggatcggcg gcgtgcactt ccgctacctg gaggccatga ccctggtcgg cctgttcttc
     8761 ctcctggtga gcgtgccttc ggccatcatc atccgacaac tggagcgacg ctatgccgca
     8821 gtctgaaccc tcccccggca ccgaccagca cgagcaggag ccgctcatcc gcttcgacgg
     8881 ggtggtcaag cggttcggcg ccaacaccgt cctcgaccac ctggacttca ccgtcgcccc
     8941 cggcgaacgg gtcaccctca tcggcccgag cggttccggc aagaccacca tcctgcgtct
     9001 gctgatgacg ttggagcagg tcaccaacgg cgtcatctgg gtcggcgggg aaccgctcag
     9061 ccacatgcac cggggcggcc gcctggtccg cgccaacgag cagcaccagc gccgggtgcg
     9121 caggcagatc ggcatggtct tccagcagtt caacctgttc ccgaacatga ccgtgcggca
     9181 gaacatcacc gaggggcccg tccacagcct gggcgtgccc cgcgtggagg ccgaccggcg
     9241 ggccgcggaa ctgctggaga tggtggacct cgccgacaag atcgacgccc accccaccca
     9301 gctgtccggc ggccagcagc agcgcgtcgc catcgcgcgc gccttggcga tgagcccgag
     9361 aatcctgctg ctggacgagg tcacctccgc cctcgacccg gaactggtca ccggagtcct
     9421 cgacgtgctg cgcaccgtcg ccgagaccac cgacatcacc atgctgctgg tcacgcacga
     9481 gatgcagttc gcccgggacg tctcccaccg ggtgctgatg ttcgaccagg gccgcatcgt
     9541 cgaggaaggg ccgcccgagc agatcttcgg cgacccccgc caggagcgca cccgcgcctt
     9601 cctgcacgcg gtcctggact agggccccgc cgggccggta ccgcgcaccc ccgcggcacc
     9661 ggcccggcgg cgcggacgag gaggagcccc gggaagggtt ccccgcccgg catgacccac
     9721 cacgcggctg ggtacactcc ccccggacaa gagaacaggg atgcgtctgg tgcaggcaat
     9781 ccggtcgaaa cccggagcgg tcgcgccact gtggacgggc acaagcccgt gagccagaaa
     9841 actcacccga cgcgtgtggt ctctgtggga cgcgtaatcc tgtaaggagt gaccatgtca
     9901 gccgtccatg ccccccgggt cggcgcggag ttgggccccg tcccgctcgc gctcaccgcc
     9961 actgcggtga ccgtcctcgc cctcctcgtc ctctacctgg tcggcttcga ccagggagcc
    10021 gtctcccgca cgggaatgta cctgcacgaa ctgatgcacg acggccgcca cctcatgggg
    10081 gtcccgtgtc actgacctcc cccgccgtct ccccgccggt ccgtccgctg cggggcacct
    10141 acctgcaact gctgctccgc gggctgctgg ccggactgct ggccgggctg gtcgcgggcg
    10201 ccgtcgcctt cctcctcggc gaaccccaga tcgaggcggc catcgccctg gaagagcagg
    10261 cagccgtctc ccacgaccac ggagcagacg aaggccacgc ccactcccac ggagaggacg
    10321 cactggtcag ccgtaccgga cagcaggtgg gactgttcct cgccaccggc cttgcgggta
    10381 tggcgctggg agcgctctac gcctc
//
