LOCUS       NZ_CP116958            11227 bp    DNA     circular CON 27-MAR-2024
DEFINITION  Streptococcus pasteurianus strain WUSP074 chromosome, complete
            genome.
ACCESSION   NZ_CP116958
VERSION     NZ_CP116958.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pasteurianus
  ORGANISM  Streptococcus pasteurianus
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2371672)
  AUTHORS   Wang,S. and Wu,Z.
  TITLE     The identification of Streptococcus pasteurianus by multiplex PCR
            assay and the characteristics of pathogenicity and antimi-crobial
            resistance of this zoonotic pathogen
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2371672)
  AUTHORS   Wang,S. and Wu,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) College of Veterinary Medicine, Nangjing
            Agricultural University, Weigang No. 1, Nanjing, Jiangsu 210095,
            China
REFERENCE   3  (bases 1 to 2371672)
  AUTHORS   Wang,S. and Wu,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2022) College of Veterinary Medicine, Nangjing
            Agricultural University, Weigang No. 1, Nanjing 210095, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 500.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023162015.2-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 06:55:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,411
            CDSs (total)                      :: 2,319
            Genes (coding)                    :: 2,195
            CDSs (with protein)               :: 2,195
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 56 of 124
            Pseudo Genes (incomplete)         :: 84 of 124
            Pseudo Genes (internal stop)      :: 36 of 124
            Pseudo Genes (multiple problems)  :: 40 of 124
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:54:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 89361
            Orig. end                         :: 100588
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116958.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11227
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11227
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11227
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6227](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6227
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    54..69
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 20.83"
                     /tool="antismash"
     misc_feature    complement(54..70)
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.55"
                     /tool="antismash"
     misc_feature    complement(303..319)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.69"
                     /tool="antismash"
     gene            complement(356..814)
                     /locus_tag="M0P28_RS00670"
                     /old_locus_tag="M0P28_00670"
     CDS             complement(356..814)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013642636.1"
                     /locus_tag="M0P28_RS00670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M0P28_00670"
                     /product="hypothetical protein"
                     /protein_id="WP_117480067.1"
                     /transl_table=11
                     /translation="MTIEYVDIAKQTGKSISTIKKWRKKIEKLSGYEFTKTKARVSRHS
                     VQVFFDFSKDEVAKFIELSKEVDKTKDLDSSIKKVWGDLNTQQAQSLEEDLLELEFDFY
                     NFKKEINKTVTALQADNCILSQRLTSMLERLNELEDDQNKGFFKKFKK"
     misc_feature    864..881
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.23"
                     /tool="antismash"
     gene            954..1589
                     /locus_tag="M0P28_RS00675"
                     /old_locus_tag="M0P28_00675"
     CDS             954..1589
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1148: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030883.1"
                     /locus_tag="M0P28_RS00675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M0P28_00675"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_117480069.1"
                     /transl_table=11
                     /translation="MNDYLIQNFGKNVAQLRKKKGVTQAELAEVIGVKKAAISKIELGT
                     SYPTFANLDKIARYFKATPNELFGTSTDIELERAVIKTDKYSEKAEKIIRAVSKIEEYS
                     EKADRILYTLERIDELERILDSSQDYSNEVIYILDRLTYAPIMETDKGEPLYRDDFGHL
                     TADPSEGVKYARHLSQLQLLLRQADKIKEIANNVNFILKHKDLLDKDS"
     misc_feature    complement(1921..1936)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 19.72"
                     /tool="antismash"
     misc_feature    complement(1936..1945)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     gene            1999..2952
                     /locus_tag="M0P28_RS00680"
                     /old_locus_tag="M0P28_00680"
     CDS             1999..2952
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /locus_tag="M0P28_RS00680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M0P28_00680"
                     /product="IS30 family transposase"
                     /protein_id="WP_003067143.1"
                     /transl_table=11
                     /translation="MQNYYTPKSKHLTLTERRMIERWLQEGLSNREIARRLAKAPQTIH
                     NEVKRGQVRQQVRKGKFEVIYSADFAQKAYQNNRKRSVKQVSLTKGLKEKITHYIEQKY
                     SPEMMIKSKGIPVPISTIYYWIHHGHLGLTKADMLYPRQEKAKKKHASPNFKPAGKSIE
                     ERPESINKRENIGDFEIDTVIQTRAKNECLLTLTDRKSRYQIIRLIPDKSAFSVNQALK
                     AILKDYQMNSITADNGAEFSRLAEVFDPTHIYYAHPYSSWERGTNENHNRLIRRWLPKG
                     SKNATQQQVAFIENWINNYPKKLFNYKSPKEFLQTG"
     gene            3162..3455
                     /locus_tag="M0P28_RS00685"
                     /old_locus_tag="M0P28_00685"
     CDS             3162..3455
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="M0P28_RS00685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="M0P28_00685"
                     /product="hypothetical protein"
                     /protein_id="WP_117480071.1"
                     /transl_table=11
                     /translation="MKCAYENKDLIFLLNIDINNKNSYCEQCFFQLLSDEFAQTLKSSN
                     TNKLELQNNIFNKLELNQNQYGVYHKITKKWEIIDIFPVPGCKGLKHEVTYS"
     gene            3436..3915
                     /locus_tag="M0P28_RS00690"
                     /old_locus_tag="M0P28_00690"
     CDS             3436..3915
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /locus_tag="M0P28_RS00690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M0P28_00690"
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="WP_117480074.1"
                     /transl_table=11
                     /translation="MKLLIVNGSGRQYNNSRLVCKQVSILCEDLKIKSRTIDLYDVYNT
                     KDKLKKIFQNYNCILWVSPEYNRSFSANIKHFIEILGVELFQSKINGIISTSIGESSRA
                     GLNQMANLLFSMDTLIVPPGIEYNHILHVDDTTIDKILILLKNMQYFSKKIYGSN"
     gene            3902..5011
                     /locus_tag="M0P28_RS00695"
                     /old_locus_tag="M0P28_00695"
     CDS             3902..5011
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /locus_tag="M0P28_RS00695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M0P28_00695"
                     /product="CocE/NonD family hydrolase"
                     /protein_id="WP_248488384.1"
                     /transl_table=11
                     /translation="MVQIKEINLKGLHTLYCEGNDTFFVIRTPYNSKGYKKWIEEVRKR
                     YGYTVIVQDVRGRYGSMGNWKPYFYENEDAQILAKFILQHNVSRVIIFGSSYEALFNGE
                     SFRLADYYWWNMVHGKGSESNLSLEIPYAKLEMPKLKLPKKNNVVYPDVPVILIGGWND
                     GYYQYAFEDYINWKNPNAHLYIGNFGHELYMINKLFDEVDWYKNLEIIGRSEKIEYKNQ
                     KGYYSQYYGKTQDFINIEISYCEKVYDNAYLLKNNYFYINNKNTFNVVIKGLSVVKSLK
                     LQHNFLETSIYGQLICIDNNIKISITEFILEKGCTETILPPLLIKNNGNIRLEFLLSTS
                     MFPRYTRPIKDSQIVEVKKIIVGGTRYEG"
     gene            5001..6227
                     /locus_tag="M0P28_RS00700"
                     /old_locus_tag="M0P28_00700"
     CDS             5001..6227
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="M0P28_RS00700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M0P28_00700"
                     /product="YcaO-like family protein"
                     /protein_id="WP_117480078.1"
                     /sec_met_domain="YcaO (E-value: 1.1e-45, bitscore: 153.9,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVNSALYNPYTGIIKNISLLQNELTPDYYIIKSSVTDRKKFDTE
                     FLFDEVGAGCSLSYEKAILSSIGEAVERYCGNIPMNEIIEKSKQELENSEEKYFPIESF
                     ISYPESTKKAVGFPFKKLENKEKIPWIRGYNLFNKQEEYLVPITMVYVNYKTEGKNYNF
                     PNLSGLASGMSLNQAISGAVLEIIERDASIRWWYFDAEEKLIRKDITDNYVFLSYQIDA
                     IVPTVASFLFDLKNELVSVGFASRFDFEEAIEKSKAEAVQLNMNMVNILNDNLSDIDPV
                     KKYLKPYRKDRNYIDSFKKDKTDMFDLIHNIQYYVDKRAFKKMSNRMSQTVEKIQEEKI
                     IKNADELFTYFKKSKQEIIIVEITTKDIESMGWYTVRVLIPGFIINTPTAFLPIDKKII
                     RNRMPLPHS"
     gene            6238..6783
                     /locus_tag="M0P28_RS00705"
                     /old_locus_tag="M0P28_00705"
     CDS             6238..6783
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /locus_tag="M0P28_RS00705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M0P28_00705"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_117480080.1"
                     /transl_table=11
                     /translation="MASYSQAMYQLVADVVEYQSLFFGRHKNILIIGLNNERQNMERYP
                     NLNYTILDKNIEYEKLDFKYYNFIQCDLNKEFPHLNSKFDLIIGNFILEYIKDKREFLA
                     NLEKVLSSNGELVLCLWYHNRELDNDFKMKLKKYFSKSNLQIYSLKLFEGENVDELSLF
                     PKVLQLQVKGRKNEKIMG"
     gene            7069..8214
                     /locus_tag="M0P28_RS00710"
                     /old_locus_tag="M0P28_00710"
     CDS             7069..8214
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1237: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826497.1"
                     /locus_tag="M0P28_RS00710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M0P28_00710"
                     /product="IS110 family transposase"
                     /protein_id="WP_041973040.1"
                     /transl_table=11
                     /translation="MLYVGIDVAKNKHDVTVIDNTGKMVLKPITITNRKQGFELLHNTL
                     KQLNQNCLIAMEDTGHYALNLLTFLHQNNYQIYTYNPLLIKEFTKSLSLRKTKTDKKDA
                     RTIALKLLSDPNRALFMHDNRQEELKIMTRHMNRLKKHQSDWKVQYTKCLDIIFPELHQ
                     VVTKHSDYVYELLKGFPSPEKMVTAGFNKLIEIKRLTAKHALDILKLAPDSIGTTSFAR
                     ELELIEIIENIQHYEKLIKQAEKKMDELMAELNSVITTVPGISNRLGSVILAEIRNINT
                     FNNPAQLQAFAGLEPAIYQSGQLDTRGKMVKRGSSHLRWALIQAAIKVARYSPTFKAYF
                     RTKLAQGKHYNVAISHVAKKLIRVLFHLLQNNEAFEEDKLR"
     gene            8341..9468
                     /locus_tag="M0P28_RS00715"
                     /old_locus_tag="M0P28_00715"
     CDS             8341..9468
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /locus_tag="M0P28_RS00715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M0P28_00715"
                     /product="MFS transporter"
                     /protein_id="WP_274513095.1"
                     /transl_table=11
                     /translation="MLIIFYFSSQFQSTTQIAYIMLADSLPFLLLGPFIANLVNKYNRK
                     VLMSLSLGFDSLLTLIFLTVIHLGYNNFLFTLILISVNNVTTSLFQISETSYFPLLVSN
                     KELAKYNSTLFFMSSLSLIISPNISLVLSNQSLILLILCFIWFVQIIALINSLLLKNIS
                     IIPKEESENKKLSLISSFLYTFKSKTLFIALLVLIIGNFFDAPLETVILGKYTAINKVS
                     QVGLLFSMSGLGSLLGSSVVNMVSKDTQKFELIMKLSSILIIFSGLALFLFDNYIIYLI
                     TFICLYFSMGIRTIYIITFRQIKSPSEILGSINTLFKYIAFGISPISIWLFSQLGKFVP
                     DNRLLQLIGLGFIVAGLISILSLKNDGKLLLEEQK"
     gene            9465..10289
                     /locus_tag="M0P28_RS00720"
                     /old_locus_tag="M0P28_00720"
     CDS             9465..10289
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="M0P28_RS00720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="M0P28_00720"
                     /product="hypothetical protein"
                     /protein_id="WP_117480084.1"
                     /transl_table=11
                     /translation="MKYGLMLPLIEGEVTENIDLLKNIINTNIDSIWVRDVVISSKDNN
                     DNGSRVSPFLHLSKLAEANLNNYIFGTGVLSALYRNVEVTIQDMTSLSELHPNSNFIYA
                     VGIGGKKEAFDALQFNWEKKDLYYDKWLQKYKVYYESQFYGGSPKNQKSNLIWSKNQKL
                     PKLTVATRNLEILQKNHSIIERNLIWFSDVDTIKDLKEKFPDIELNMFLSLKIIDGENT
                     IRYVDKGQKFFEISVDKLKDLSKEYDSLGIHRLILSFGGNVGDSDFHLLNTV"
     gene            10336..10884
                     /locus_tag="M0P28_RS00725"
                     /old_locus_tag="M0P28_00725"
     CDS             10336..10884
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /locus_tag="M0P28_RS00725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M0P28_00725"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_117480086.1"
                     /transl_table=11
                     /translation="MMNTTRLLVNFSKNTILETDRLVLKPLSVNDLDDYHEYSSDDETL
                     KYDYLPHKTLKESEEGLVTWHLSAPLGKYGLFLKSSGKLIGNINFRPETNEKVFVGYTL
                     NKNYWKKGYATEALQELISLSFTQLGIKQILAKVHSENGASIALLLRLGFSLVNVESNV
                     KHYRGYTFDELTYEKRKDI"
ORIGIN
        1 ttgaccaaag caatatcatc agctttagca ctcaattcta gtgatacaaa ttcagacccc
       61 ttagtggtac gtgtataaac accgacatta aggttggtaa atttaacagt aactgaacct
      121 tgttcagcta acttttcctt agaaataata ggtgcttcac catctggatt cggcacttta
      181 acagccactt tttcatagcg tagtcttggt agaactacca tgtatttcca tcctagatga
      241 acacgttcat cattattaag ttcataccaa tcttggattt caacgagttg gtaatctttt
      301 tcttcccagt ttttaaccat aacttcggta gttttaattg acatattttg tcctcctatt
      361 ttttaaattt cttgaagaat cctttatttt ggtcatcttc taactcattc aaacgctcta
      421 acatacttgt taaacgttgt gataaaatac aattgtctgc ttggagagca gttactgttt
      481 tgttgatttc ttttttaaag ttgtagaaat caaattcaag ttccaataaa tcctcttcta
      541 aactttgcgc ttgctgggta tttaggtcac cccaaacctt tttaatagaa ctatctaaat
      601 cttttgtctt atcaacttct ttagataact caatgaactt agcgacctca tcttttgaaa
      661 agtcaaagaa tacttgtacg ctatgccgac tgactctagc tttagttttc gtaaactcat
      721 agccagatag tttttcaatc tttttacgcc actttttaat ggtgctaata gattttccag
      781 tttgtttcgc tatgtccaca tattcaatag tcatcacctc ttgttttttg ttttagatat
      841 tttatatctt tcaaagatat tataatctta taaagtggat aagtcaagat atttttatgt
      901 ttttttagat attttatatc tttttatgat ataatcttac tagaggtgat aaaatgaacg
      961 actatttaat acaaaacttc ggaaaaaatg ttgcacagtt aagaaagaaa aaaggagtta
     1021 ctcaagcaga actagcagaa gttattggag tgaaaaaagc agctatttct aaaattgaat
     1081 taggcacaag ctatccaaca ttcgcaaatc tagataaaat tgctaggtat tttaaagcaa
     1141 cacctaatga actttttgga acaagtacgg acattgaatt agaaagggct gttattaaga
     1201 cggataagta tagtgaaaaa gctgaaaaaa ttatccgtgc tgtatcgaaa atagaagaat
     1261 atagtgagaa agcggatcgg attttgtata ctttagaacg tattgatgag ttagaaagaa
     1321 tcctcgattc ttctcaagat tattcaaatg aagttatcta tattcttgat aggttaactt
     1381 atgcaccaat tatggaaact gataaaggag aaccattata tcgtgatgac tttggtcatc
     1441 taactgcaga cccaagtgaa ggagttaaat acgctcgtca tctttcacaa ctccaattac
     1501 ttttaagaca ggctgataag attaaagaaa ttgcaaataa tgtcaatttt atcttgaaac
     1561 ataaggattt attggataag gattcttgat ttaaaaaagt ttttaaacaa aaaaaacaag
     1621 gcttaaaatg tttaacaagc cttgtttttt gtaaacaata aatagaaaat ttgcaatatt
     1681 ttgaaatgag catatttttt gacttttcag tatacggtta gatatactga aagtggtttt
     1741 ataattaaac cttatatcca cataaaaaag atagtaaaaa gtattctcct atgggatgga
     1801 agaatactat aagttaacta aataaattat agcagaaaaa agaggtgttg catatgtcaa
     1861 ataaaaaatt accaacaatt gaagaacttt ctcaacaaag gaaagttcta aaatgaagtt
     1921 agccacctaa gggtatcaaa aaacatctca gagagatata attgtaatca ccacaacaac
     1981 aattagaaag actctgagat gcaaaactat tatacaccaa aaagtaaaca tttaacacta
     2041 actgaacgta gaatgattga acgttggctt caagaagggc tctcaaaccg tgaaatcgct
     2101 aggagattag ctaaagctcc tcaaaccatt cacaacgaag tcaaacgtgg tcaggttaga
     2161 caacaagtgc gtaaaggaaa atttgaagtg atctactcag ctgattttgc tcaaaaagcc
     2221 tatcaaaaca atcgcaaacg ttctgttaaa caagtctccc taaccaaggg actcaaagaa
     2281 aagataactc actacatcga acagaaatac tctcccgaga tgatgataaa gtcaaaaggg
     2341 atacctgttc ccatctctac catttactac tggattcatc atggacactt aggattgacc
     2401 aaggctgata tgctttatcc tcgacaagag aaagctaaga aaaagcatgc tagtcccaat
     2461 tttaagccag ctggaaagtc tattgaggaa cgaccagaaa gcattaataa gcgtgagaat
     2521 atcggtgatt ttgaaattga tacagttatt caaacacggg caaaaaacga gtgtctgttg
     2581 actctaaccg atagaaagag tcgttatcaa atcattcgac tcattcccga taagtccgcg
     2641 ttttcagtca atcaagctct gaaagcaatc ctcaaggatt atcaaatgaa ctctatcaca
     2701 gctgataacg gggctgagtt cagtcgttta gcagaagttt ttgaccctac tcatatctac
     2761 tatgcccacc cgtattcttc ttgggagcgt ggtactaatg agaatcataa tagactcatc
     2821 cggcgttggt tgcctaaggg aagcaaaaat gcgactcaac aacaagtcgc atttattgaa
     2881 aactggatta acaactatcc aaagaaacta ttcaattata aatctcctaa agagttttta
     2941 cagactggct aatttgaact tgaaatttgg cctttctcaa caaatggaaa gagattataa
     3001 ttctggctct gataaaatct ctacagcttt gaattgtcat cattcacttg ttcataactc
     3061 atttgaatgg gtagatgaag ataaataatt tttatcttct taattaataa ttaaatattt
     3121 tactatcttt ttattggata ttaaatgttg gaggtaattt tatgaagtgt gcatatgaaa
     3181 ataaagactt aatatttctt ctaaatattg atataaataa caaaaatagc tattgtgaac
     3241 agtgcttttt tcagttgcta tctgatgaat ttgcacaaac attaaaaagt agtaatacaa
     3301 ataaacttga gttacagaat aatatattta ataaacttga acttaatcaa aatcagtatg
     3361 gagtctatca taaaatcaca aaaaaatggg aaattataga tatttttcca gttccaggtt
     3421 gtaaaggatt gaagcatgaa gttacttata gttaatggtt caggcagaca atataataac
     3481 tcacggcttg tatgcaagca ggttagtatt ctgtgtgaag acttaaaaat aaagagtcga
     3541 actatagatt tatatgatgt atataatact aaagataagt taaaaaaaat attccagaat
     3601 tataattgta ttttgtgggt aagccctgaa tataatcgga gtttttcagc aaacattaaa
     3661 catttcattg aaatattagg agttgaatta tttcaatcaa aaattaatgg aattatttca
     3721 acgtctattg gagaatcctc tagggcagga ttaaatcaaa tggcaaattt attattctca
     3781 atggatacgt taattgttcc accgggaata gagtataatc acattttaca tgtagatgat
     3841 acaactattg acaaaatttt gatattatta aaaaatatgc aatattttag taaaaaaatt
     3901 tatggttcaa attaaagaaa ttaatctaaa aggtttacat actttatatt gtgaaggaaa
     3961 cgataccttc tttgttatcc gaactccgta taattctaaa ggctataaaa aatggataga
     4021 agaagttaga aaaagatatg gatatactgt aatagtccaa gatgtacgcg gtaggtatgg
     4081 ttctatggga aattggaaac catattttta tgaaaatgag gatgctcaaa tattagcaaa
     4141 atttatttta caacataatg ttagtagggt tattatattt ggttcttcat atgaagcttt
     4201 gtttaatggt gaaagtttta gattagctga ctattattgg tggaatatgg tacatggtaa
     4261 aggaagtgag agtaatctgt cgttagaaat accatatgct aaattagaaa tgccgaagct
     4321 aaaacttcct aaaaaaaata atgtagtata tccagatgtt ccagtaattt taattggagg
     4381 atggaatgat ggttactatc aatatgcttt tgaagattat ataaattgga aaaatccaaa
     4441 cgcacatctc tacattggta attttggtca cgaactttat atgataaata aattatttga
     4501 tgaagtggac tggtataaaa atttagaaat tataggaagg tctgaaaaga ttgagtataa
     4561 gaaccagaaa ggatactata gccagtatta cggtaaaact caagatttta tcaatattga
     4621 aatatcttac tgtgaaaaag tttacgacaa tgcctattta ttaaaaaata attattttta
     4681 tataaataat aaaaatactt ttaatgttgt cataaaagga ctatcggtag taaaaagtct
     4741 taaattacaa cataattttt tagaaacatc catttatggt caactaatat gtattgataa
     4801 taatataaag atttctataa ctgaatttat attggaaaag ggatgcacgg agaccatttt
     4861 gcctccgtta ttaataaaaa ataatggtaa cataagatta gaatttctat tatctacaag
     4921 tatgtttcca aggtacacaa gaccaattaa agattcacaa attgtagagg taaaaaaaat
     4981 tatagtaggt ggaactcgct atgaaggtta attcagcatt atataatccg tatacaggaa
     5041 taattaaaaa tataagtcta ttacaaaatg aacttacacc agattattat attataaagt
     5101 ctagtgtaac agatagaaaa aaatttgata cagaattttt atttgatgaa gttggtgcag
     5161 gttgttcgct cagctatgaa aaagcaattt tatcaagtat tggtgaagca gttgagagat
     5221 attgtgggaa cataccaatg aacgaaatta tcgaaaaaag caaacaagaa ttagaaaatt
     5281 ctgaagaaaa atattttcca atagaatcgt ttatctctta tccagaatct acaaaaaagg
     5341 cggttggttt tccttttaaa aaattagaaa ataaagaaaa aattccatgg ataaggggat
     5401 ataatctatt taataagcaa gaagagtacc ttgttcccat tacaatggtt tatgtaaatt
     5461 ataaaacgga aggtaaaaat tataactttc caaatttatc agggcttgct agtggtatga
     5521 gtttaaatca ggcaatcagt ggtgcagtat tagaaattat agaaagagac gcttctattc
     5581 gatggtggta ttttgatgca gaagaaaaat taataaggaa agatatcaca gataactatg
     5641 tatttttgtc gtatcaaatt gatgcgatag ttcctacagt agcatcattt ttattcgatt
     5701 taaagaacga attagtatca gttggttttg cttctcgatt tgattttgaa gaggctattg
     5761 aaaaatcaaa agcagaagcg gtgcaattaa atatgaatat ggtgaatatt ttaaatgata
     5821 atttgagcga tattgaccca gtaaagaaat atttaaagcc atatagaaaa gatagaaatt
     5881 atattgattc ttttaaaaaa gataaaacag atatgtttga tttaatacat aatattcaat
     5941 attatgttga taaacgagca tttaaaaaaa tgagtaatag aatgagtcaa acagttgaaa
     6001 aaattcaaga agaaaaaatt ataaaaaatg cagatgaact ttttacttat tttaaaaaat
     6061 ctaaacaaga aattattatt gtagaaatta ctacaaaaga tatagagagt atgggatggt
     6121 ataccgtaag agtacttatt cctggtttta taataaatac acctactgcg ttcctaccaa
     6181 tagataaaaa aataatacga aatcgaatgc ctttaccaca tagttaggag ataaaatatg
     6241 gcttcatatt cacaagctat gtaccaacta gtagcagatg tagttgagta tcaatcttta
     6301 ttttttggta gacacaagaa tattctaatt ataggattaa ataatgaacg acaaaatatg
     6361 gagaggtatc ctaacctaaa ttatacgatt ttagataaaa atatagaata tgaaaagctt
     6421 gattttaagt actataattt tattcaatgt gaccttaata aggagtttcc gcacttaaat
     6481 tcaaaatttg atttaattat tggaaatttt attttagaat atataaaaga taagagagaa
     6541 tttcttgcaa acctagaaaa ggtattatca agtaatggag aattagtttt atgtctttgg
     6601 tatcataata gagagctaga taatgatttt aaaatgaaat taaaaaaata ttttagtaag
     6661 agtaatttgc aaatatattc actgaaacta tttgaggggg aaaatgtaga tgaattatct
     6721 ctttttccca aagtgttgca attacaagta aaagggagaa aaaatgaaaa aattatggga
     6781 taactttttt atatctgcaa gtgttattga ttttattaat gtaaccttat attatattta
     6841 gtgcagttag ctactgccaa agcccaagtg ttatacttgg aaaagccact gtggattagt
     6901 ttattgtgtc caatcgcttg acgatttacg aaaggatcta accacagctt tttgttttca
     6961 ttggtaatga gtttgataac gctagacgtt taatcttcag taaggtaaca aggcaaaaag
     7021 taagtggata ccaagtgggc ttaatactta atatctggag gtacttttat gctctatgtt
     7081 ggcattgatg tcgctaaaaa caaacacgat gtgacagtta ttgataatac agggaaaatg
     7141 gtgttaaaac ccatcactat tacaaaccgt aaacaaggat ttgaactact tcataatacc
     7201 ttgaaacaac tcaaccagaa ttgtcttatc gccatggaag ataccggaca ttatgcctta
     7261 aatctcttaa cattcttaca tcaaaacaat tatcagattt acacctataa tcccttactt
     7321 atcaaagaat tcacaaagtc tctctcactt cgtaaaacga aaacggataa gaaagatgca
     7381 cgtacaatag ctcttaagtt actttcagac cctaatcgtg ccttatttat gcatgacaac
     7441 aggcaagaag aacttaaaat tatgacacgt cacatgaatc gtttaaagaa acatcaatca
     7501 gattggaaag tccaatatac caagtgttta gatattattt tccctgaact acatcaggtc
     7561 gttacgaagc actctgacta tgtttatgag ttgctcaaag gttttccttc tcctgagaaa
     7621 atggtaactg ctggctttaa taaacttatt gagattaaac gattaacagc taaacatgca
     7681 ttggatattc tcaaactagc tccagattct attggcacaa cttcttttgc tcgtgaattg
     7741 gaattgattg agattattga aaatatccaa cactacgaaa aactcatcaa acaagctgaa
     7801 aagaaaatgg atgagctgat ggctgaactc aattcggtca tcactacggt accagggatt
     7861 agtaaccgtc taggttctgt tattctagct gaaatcagaa atattaatac tttcaataac
     7921 ccagctcaac tccaagcttt tgcaggatta gaaccagcta tctaccaatc tggacagtta
     7981 gatactcgag ggaaaatggt caaacgaggg tcatcacatc tacgttgggc actcatacaa
     8041 gctgccataa aagtagctag atactcgcct acgtttaaag cttattttag aaccaagtta
     8101 gcgcaaggaa aacactacaa tgtcgctatt tctcatgtgg ctaagaaact cattcgagtg
     8161 ctgtttcatc tacttcagaa caatgaagcc tttgaagagg ataaactaag ataaaccaac
     8221 aaaaacatag atgaaaaact ttttaaaaca gttgttttaa aaggattttt gtcgtgtcta
     8281 aaacacgttc taaaaaaatt tagaacaaac tattgacaac tcttatagtt tgtctttcgt
     8341 atgctgatta ttttttactt ttctagtcag tttcaatcta cgacccaaat tgcatatatt
     8401 atgttagcag atagtttacc atttttattg ttaggtccat ttatagctaa tctggttaat
     8461 aaatataata gaaaagtatt aatgagttta tctcttggat ttgattcatt acttactttg
     8521 atttttctga cagtaataca tttaggttac aataattttt tatttacatt aatactaata
     8581 tctgttaata acgtaacaac ttctcttttt cagattagtg aaacatcata tttcccatta
     8641 ttagtaagca ataaagaatt ggctaaatat aattctacgc tattttttat gagtagttta
     8701 tcattaatta tttctcccaa catttcatta gtcttatcaa atcaatcgct catcctctta
     8761 attttatgtt tcatatggtt tgttcaaata attgctttga taaattcact gttattgaag
     8821 aatatatcaa ttattccaaa agaagagagt gagaataaaa aactttccct tatttcttca
     8881 tttctttata catttaaaag taagacacta tttattgctc tactggtact cattataggg
     8941 aatttttttg atgccccctt agaaactgta attttgggta aatatacagc aattaataaa
     9001 gtgtctcaag taggacttct attttccatg agtggtttag gctcactatt aggatcttca
     9061 gtagttaata tggtatcaaa agatactcaa aaatttgaac taattatgaa actttcttca
     9121 atattaatta ttttcagtgg tcttgctctt ttcttatttg ataattatat catctatcta
     9181 attacattta tttgcttata tttttcaatg ggcataagaa ctatatatat tataactttt
     9241 aggcaaataa aatcaccatc tgaaatttta ggtagcataa atactctatt caagtatata
     9301 gcatttggaa tatctcctat ttccatatgg ttgttttcac aattaggtaa gtttgtgcca
     9361 gataatagat tattacaatt aattggtttg ggttttatag ttgcaggttt aatctcaatc
     9421 ctatctctaa aaaatgatgg aaaattgttg ttggaggaac aaaaatgaag tatggactta
     9481 tgctaccttt aatagaggga gaagtaacag aaaatattga tttactaaaa aatattatca
     9541 atacaaatat agatagtatt tgggtaaggg atgttgtgat atcaagcaaa gataataatg
     9601 ataatggaag tagagtatca ccttttttgc atttatcaaa attggcagaa gcaaacttaa
     9661 ataattacat ttttggtacg ggagttttga gtgctctcta tcgaaatgtg gaagttacta
     9721 tacaagatat gacatcatta tctgaattgc atccaaactc aaattttata tatgctgtgg
     9781 gtataggtgg aaaaaaagaa gcctttgatg ccttacagtt taattgggaa aaaaaagatt
     9841 tatattatga taaatggtta cagaaatata aagtctacta tgaaagtcag ttctatggtg
     9901 gttctcccaa aaatcaaaaa tcaaatttaa tttggagtaa aaatcaaaaa ttacctaaat
     9961 tgacggtagc aacacgaaat ttagaaattt tacaaaagaa tcattccata atagaaagaa
    10021 accttatctg gtttagtgat gtagatacaa taaaagattt aaaagagaaa tttcctgata
    10081 ttgaattaaa tatgttttta tcactaaaaa taattgatgg agaaaataca ataagatatg
    10141 ttgataaggg gcaaaagttc tttgaaattt ctgtagataa gttaaaagat ttatcaaaag
    10201 aatatgattc tctaggtatc catcgattaa ttttatcgtt tggtggaaat gttggcgata
    10261 gtgatttcca tctattaaat actgtttaag cattttaaag gattaattaa atatacaaga
    10321 aagagaaaca gatgaatgat gaatacaaca agattattag taaatttttc taagaatact
    10381 atattagaaa cagataggtt agtattaaaa cctttaagtg ttaatgattt agatgattat
    10441 cacgaatatt ctagtgatga cgagacacta aaatatgact atttacctca taaaacattg
    10501 aaagagagtg aagaaggttt agtgacatgg catttatcag cacctttagg gaaatatgga
    10561 ctttttttaa aatcaagtgg taaattaatc ggaaatatta attttagacc tgagaccaat
    10621 gaaaaagttt ttgttggata cactttaaat aaaaactatt ggaagaaggg ctatgctaca
    10681 gaagcattac aagaattaat atcattatca tttacgcaat taggcattaa acaaatttta
    10741 gcaaaagttc attcagaaaa tggagcctct atagcacttt tattgagatt gggattttct
    10801 ttagttaatg ttgaaagtaa tgttaaacat taccgaggtt atacttttga tgagttaact
    10861 tatgaaaaaa gaaaagacat ttaagatatt ttgagtagtt tctaaaaagt atctaaaaaa
    10921 taaaaaaaat taatattttt aaggggatag aatgataaga agcctattaa attagtcttt
    10981 ttagctttaa aaaataacgg tgttgcactc aaaatccagt gtccgcaagg acgtgccggt
    11041 tcgaccccgg ccgccggtat aaaatagcaa aaagtcctaa cctataggac tttttgctat
    11101 ttttctgtta tttgttaaga ataatgggat tttttattct tgaatttaca atataagtgc
    11161 ataataaaaa ctcataagat tttctagtaa aatagactta accaaacctc ttattacgaa
    11221 aggaaat
//
