LOCUS       NZ_JALDUX010000002     20876 bp    DNA     linear   CON 12-MAY-2024
DEFINITION  Streptococcus mitis strain S25 NODE_2_length_327649_cov_162.316239,
            whole genome shotgun sequence.
ACCESSION   NZ_JALDUX010000002
VERSION     NZ_JALDUX010000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus mitis
  ORGANISM  Streptococcus mitis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 327649)
  AUTHORS   Palmer,K., Luke,J. and Madison,Y.
  TITLE     Whole genome sequencing of viridans group streptococci associated
            with infective endocarditis and the human mouth
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 327649)
  AUTHORS   Palmer,K., Luke,J. and Madison,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2022) Biological Sciences, The University of Texas
            at Dallas, 800 W Campbell Rd, BSB 12, Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 209x
            Sequencing Technology             :: Illumina NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023109755.1-RS_2024_05_12
            Annotation Date                   :: 05/12/2024 04:19:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,953
            CDSs (total)                      :: 1,906
            Genes (coding)                    :: 1,839
            CDSs (with protein)               :: 1,839
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 45 of 67
            Pseudo Genes (internal stop)      :: 16 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:27:13
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 256263
            Orig. end                         :: 277139
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALDUX010000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20876
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="1"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..20876
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20876
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10876](+)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10876
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     misc_feature    complement(530..544)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.28"
                     /tool="antismash"
     misc_feature    1175..1188
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: strong, score: 23.59"
                     /tool="antismash"
     gene            2228..2971
                     /locus_tag="M1F92_RS05980"
     CDS             2228..2971
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /locus_tag="M1F92_RS05980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class A sortase"
                     /protein_id="WP_033681684.1"
                     /transl_table=11
                     /translation="MSHKKNNKKIKRKNLLTNILAVFLILISIALIFNAKIRDAFIAWN
                     SNKYQVSQVSKEKIEENQDTEGNFDFDSVKAISSEAVLASQWDNQKLPVIGGIAIPELE
                     INLPIFKGIANVNLLYGAGTMKRDQEMGKGNYSLASHHIFTGDNASQMLFSPLDRAKKG
                     MKIHLTDKNKVYTYEISEVKRVTPDRVDEIDDRTGVDEITLVTCEDAAATERIIVKGDL
                     KETKDYSQTSDEILTAFNQPYKQFY"
     misc_feature    3016..3030
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.72"
                     /tool="antismash"
     misc_feature    3048..3059
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.77"
                     /tool="antismash"
     misc_feature    3076..3091
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.55"
                     /tool="antismash"
     gene            3114..3911
                     /locus_tag="M1F92_RS05985"
     CDS             3114..3911
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /locus_tag="M1F92_RS05985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="formate/nitrite transporter family protein"
                     /protein_id="WP_247932932.1"
                     /transl_table=11
                     /translation="MVSSEFISKIEFACKKKESLYSQSKFKYAIRSMFAGAFLTFSTAA
                     GAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISWRK
                     TAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELILLEAIL
                     ANIFVNIAILSFVLVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVAADTI
                     ANFGVGNMLRHWGVTFVGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            4059..5339
                     /locus_tag="M1F92_RS05990"
     CDS             4059..5339
                     /NRPS_PKS="Domain: Aminotran_1_2 (58-224). E-value:
                     2.9e-12. Score: 38.3. Matches aSDomain:
                     nrpspksdomains_M1F92_RS05990_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1168: O-succinylhomoserine sulfhydrylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /locus_tag="M1F92_RS05990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="WP_247932933.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.5e-11, bitscore:
                     38.3, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 1.1e-16,
                     bitscore: 55.2, seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_5 (E-value: 2.1e-10, bitscore:
                     34.2, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRDFKFETLQLHAGQVVDPATKSRAVPIYQTTSFVFDDTQEGAD
                     LFALRKPGNIYTRITNPTTAAFEERIAALEGGVGALATASGMAAVTYTILALAHAGDHV
                     VAASTIYDGTFNLLKETLPRYGITTTFVDVDNLEEVEAAINDNTKLVLIETLGNPLINI
                     PDLEKLAEIAHKHQIPLVSDNTFATPYLINVFSHGVDISIHSATKFIGGHGTTIGGVIV
                     DSGRFDWEASGKFPQFVEEDPSYHNLSYTRDVGAAAFIIAVRVQLLRDTGAALSPFNAF
                     LLLQGLETLSLRVERHVQNAEKIVDFLVNHPKVEKVNYPKLADSPYHALAEKYLPKGVG
                     SIFTFHVKGGEAEARKVIDHLEIFSDLANVADAKSLVVHPATTTHGQLSEKDLEAAGVT
                     PNQIRLSIGLENVEDLIEDLRLALEKI"
     aSDomain        4233..4730
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_M1F92_RS05990_Aminotran_1_2.1"
                     /evalue="2.90E-12"
                     /label="M1F92_RS05990_Aminotran_1_2.1"
                     /locus_tag="M1F92_RS05990"
                     /protein_end="224"
                     /protein_start="58"
                     /score="38.3"
                     /tool="antismash"
                     /translation="ITNPTTAAFEERIAALEGGVGALATASGMAAVTYTILALAHAGDH
                     VVAASTIYDGTFNLLKETLPRYGITTTFVDVDNLEEVEAAINDNTKLVLIETLGNPLIN
                     IPDLEKLAEIAHKHQIPLVSDNTFATPYLINVFSHGVDISIHSATKFIGGHGTTIGGVI
                     VDS"
     gene            5435..6709
                     /locus_tag="M1F92_RS05995"
     CDS             5435..6709
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /locus_tag="M1F92_RS05995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF2130 domain-containing protein"
                     /protein_id="WP_247932934.1"
                     /transl_table=11
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQELA
                     LAEQKAMNEQQTKLAQKNQEIAQLQSQIQNFDTEKELAKKEVEQTSNQALLAKDKEVQA
                     LENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVTQNYEAQLKAA
                     SEQVEFYKNFKAQQSTKAIGESLEHYAESEFNKVRSFAFPNAYFEKDNKVSARGSKGDF
                     IFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLVTMLEA
                     DNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVREQNIDIT
                     HFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQLRLANNKL
                     EDVSVKKLTRKNPTMKAKFEALKEE"
     gene            6720..7598
                     /gene="truB"
                     /locus_tag="M1F92_RS06000"
     CDS             6720..7598
                     /EC_number="5.4.99.25"
                     /GO_function="GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /gene="truB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145474.1"
                     /locus_tag="M1F92_RS06000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="WP_247932935.1"
                     /transl_table=11
                     /translation="MNGIINLRKEAGMTSHDVVFKLRKILGTKKIGHGGTLDPDVVGVL
                     PIAVGKATRMVEFMQDEGKVYEGEITLGYSTTTEDASGEVVAETPVLSPLDEIIVDEAI
                     ASLTGPITQIPPMYSAVKVNGRKLYEYARSGQEVERPERQVTIYQFDRTSPISYEGELA
                     RFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAEKVEA
                     GQLDFLHPLEIGTGDLVKVFLSPEEATEVRFGRFIELDQTEQELAAFEDNKLLAILEKR
                     GNLYKPRKVFS"
     gene            7708..8346
                     /gene="udk"
                     /locus_tag="M1F92_RS06005"
     CDS             7708..8346
                     /EC_number="2.7.1.48"
                     /GO_function="GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="udk"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /locus_tag="M1F92_RS06005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="uridine kinase"
                     /protein_id="WP_001181376.1"
                     /transl_table=11
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYKD
                     QSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQEP
                     QDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVIDQYLG
                     VVKPMYHQFIEPTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            8474..9814
                     /gene="xseA"
                     /locus_tag="M1F92_RS06010"
     CDS             8474..9814
                     /EC_number="3.1.11.6"
                     /GO_component="GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA]"
                     /GO_function="GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /GO_process="GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="xseA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000165.1"
                     /locus_tag="M1F92_RS06010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="WP_247932936.1"
                     /transl_table=11
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQYF
                     SLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDGVG
                     ALAIQFEQLKKKLTEEGLFQERFKQPMPQFSKRIGVVTSRSGAVIRDIITTVSRRFPGV
                     DILLYPTKVQGEGSAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVVRAIF
                     ESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDLLAHLQNQEKRMATA
                     VRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDNKQVVQA
                     RTHRLVQLSPVTKIQRSQDRLGQLDKLLRSQMALVYDAKVAEVKRLSEALLMLDTSRIV
                     ARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     misc_feature    complement(8514..8528)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 23.47"
                     /tool="antismash"
     gene            9792..10004
                     /locus_tag="M1F92_RS06015"
     CDS             9792..10004
                     /EC_number="3.1.11.6"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /locus_tag="M1F92_RS06015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="WP_000043226.1"
                     /transl_table=11
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAIAAFQKGMVLSKEL
                     QATLDKAEKTLVKVMQEDGTESDFE"
     gene            10001..10876
                     /locus_tag="M1F92_RS06020"
     CDS             10001..10876
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /locus_tag="M1F92_RS06020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_247932937.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 4.9e-56, bitscore:
                     184.1, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRPF
                     LLLEVLEALQVAIKPAHAQVAAALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFGEA
                     MAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQHLS
                     LEELQTIHANKTGKLLAYPFQAAAIIAELVPEMQVKLKTVGELIGLAFQVRDDVLDVTA
                     SFEEIGKTPQKDLQAEKSTYPALLGLEEAIAFCNQTLDQANAKLEEIAQQVPFDIGSIV
                     SVVESLRING"
     gene            10869..11684
                     /locus_tag="M1F92_RS06025"
     CDS             10869..11684
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /locus_tag="M1F92_RS06025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="WP_247932938.1"
                     /transl_table=11
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPGE
                     KIPDDTELKLKSEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQNG
                     AELVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEKEPSFASIDVSFISLSLI
                     LPALHRVLAGQGQVVALVKPQFEAGREQIGKNGIIRDAKVHQKVLESVTAMAVEEGFSV
                     LGLNFSPIQGGHGNIEFLAYLKKEESASNQVLAEIEEVVERAHSQFKNE"
     gene            11677..12108
                     /locus_tag="M1F92_RS06030"
     CDS             11677..12108
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /locus_tag="M1F92_RS06030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="arginine repressor"
                     /protein_id="WP_020900686.1"
                     /transl_table=11
                     /translation="MNKKERLEKIRRFVTDYQIGTQEEIVEHLKEAGITATQATVSRDI
                     KELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVKAQ
                     LIETFADKIFSCLADDSSILVIARSESLAEEIFEQVKNW"
     gene            12115..13782
                     /gene="recN"
                     /locus_tag="M1F92_RS06035"
     CDS             12115..13782
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /locus_tag="M1F92_RS06035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DNA repair protein RecN"
                     /protein_id="WP_247932939.1"
                     /transl_table=11
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNMM
                     LGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDDQGLEMGDEIIIRREILQNGRSI
                     SRVNGQMVNLSVLRAIGQHLVDIHGQHDQEELMRPQLHIQMLDEFGDTGFWALKETYQT
                     SFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLSLNQERDKLLNH
                     KNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYYVLEDI
                     SKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEYNLLTGN
                     NLSSEDMEAELKKLEVNLVDLAGQLASARHDLAQQLEAEIKQELQDLYMEKAQFQVRFS
                     KGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKEGKTSIVFD
                     EVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKISNEHSTVSTV
                     RLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            13784..14512
                     /locus_tag="M1F92_RS06040"
     CDS             13784..14512
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /locus_tag="M1F92_RS06040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="metallophosphoesterase family protein"
                     /protein_id="WP_247932940.1"
                     /transl_table=11
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSRR
                     VLEMVKDLVDNQGAICLSGNHEYMFLTWLDNPEESYDHYRRNGGDTTINSILGRPLDAP
                     VDGVEDTKRVTTEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKVWLR
                     KPFHEAENHTGKTIVFGHTPVYGLLNQDRGTAELWTTEDGKIGMDGGAVYGGVLHGIVF
                     TDQGMTEHHFIENDGFVAED"
     gene            14591..16414
                     /gene="lepA"
                     /locus_tag="M1F92_RS06045"
     CDS             14591..16414
                     /EC_number="3.6.5.n1"
                     /GO_function="GO:0003924 - GTPase activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /gene="lepA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1190: GTP-binding protein LepA"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /locus_tag="M1F92_RS06045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="translation elongation factor 4"
                     /protein_id="WP_001047207.1"
                     /transl_table=11
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSREM
                     QAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLAAC
                     EGAILVVDAAQGIEAQTLANVYLALDNDLEIMPVINKIDLPAADPERVRTEIEDVIGLD
                     ASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQVRVM
                     DGVVKPGDKIQLMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYIAASIKTVQDTRVGD
                     TVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQFEPETS
                     QALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMDVSNPSEF
                     PDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVNVIYQIPLA
                     EIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFIVHKDFAYER
                     GKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGGDVSRKRKLLE
                     KQKAGKKRMKAIGSVEVPQEAFLSVLSMDEE"
     misc_feature    16462..16478
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 23.49"
                     /tool="antismash"
     gene            16522..17133
                     /locus_tag="M1F92_RS06050"
     CDS             16522..17133
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260659.1"
                     /locus_tag="M1F92_RS06050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_247932941.1"
                     /transl_table=11
                     /translation="MKKSLVLATIFISSFALTACSNKSQTESSTNVITSSSSSQEASTS
                     TTTSSDKKTEETQVIGSDEYGFVKVPKSWIKFNEVQGGNDIQYSDGTDINIVTLNTFKA
                     EQFGISDNEFQKLETVRISDSIFTSKESNPEFTKVWGSKSTIGGYEAYVVNAISKSGKY
                     IITWIFKSSDGKFRYVALEGTPETLKNLLPMIEESWTIQK"
     misc_feature    17157..17166
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     misc_feature    17276..17290
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 20.19"
                     /tool="antismash"
     gene            17340..17816
                     /locus_tag="M1F92_RS06055"
     CDS             17340..17816
                     /GO_component="GO:1902495 - transmembrane transporter
                     complex [Evidence IEA]"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /locus_tag="M1F92_RS06055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="WP_247932942.1"
                     /transl_table=11
                     /translation="MGLDHFFDKNLVFCLEADNQEHLFDQVATLLEEREIVTPTYREAL
                     ITREKSFPTGLDMEFLGKDLPNVAIPHTDIAHNLAEKVVVVRLEKPVTFHNMIAPDKEV
                     EVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEALYAYIAKAIA"
     gene            17855..18160
                     /locus_tag="M1F92_RS06060"
     CDS             17855..18160
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /locus_tag="M1F92_RS06060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="WP_000590547.1"
                     /transl_table=11
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGFDVHCDAVMVKDVNE
                     DLIKGYDIFTPIAATDLGFDPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     misc_feature    complement(18171..18188)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.83"
                     /tool="antismash"
     gene            18208..19683
                     /locus_tag="M1F92_RS06065"
     CDS             18208..19683
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /locus_tag="M1F92_RS06065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="WP_247932943.1"
                     /transl_table=11
                     /translation="MDVIIELANKLFKPILDMGGPIIMLIILTVLALLFGVKFSKALEG
                     GIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLNITDVGWAPLATITWGSAWTLY
                     FLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIATAA
                     VVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFFPGLD
                     KYDFDAAKLNKKIGFWGSKFFIGFILGIVIGLMGTPHPVEGLKDAANWQEVIKGWLSLG
                     LTAGVALELFSLIGSWFIAAVEPLSQGITNVATKRLEGRKFNIGLDWPFIAGRAEIWAC
                     ANVLAPIMLIEAVLLSNVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFGTLLLPLF
                     LLSGTLIAPFATELAKGVGAFPAGVSQTQLITHSTLEGPIEKLLGWTIGNTTTGDIKAI
                     LGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            19887..20783
                     /locus_tag="M1F92_RS06070"
     CDS             19887..20783
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234200.1"
                     /locus_tag="M1F92_RS06070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aldose 1-epimerase family protein"
                     /protein_id="WP_247932944.1"
                     /transl_table=11
                     /translation="MVIELKSEQLSVQFNSFGGALSSIKDADGLEYLWQGDATYWSGQA
                     PVLFPICGSLREDTAFYIDEKGQESKGKIPRHGLVRKKEFELVEQTENSVTFAIEDDEN
                     SYQNYPYHFRLEITYIFTGKTVRTQYKIFNKETSKVMPYFIGGHPGFNCPLLDDEVYED
                     YYLEFEKEETCSVPHSFPETGMLDFQDRSPWLEGQKEVDLSYDLFSTDAVTLDELQSRT
                     IALRSRKHDKGLKVHFAEFPNLIIWSTLNKGPFIAFEPWSGLSTSLEEGDHLEDKKNVR
                     LLEHGQVDQIAFDIEIF"
     misc_feature    complement(20785..20794)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     misc_feature    complement(20797..20810)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 20.13"
                     /tool="antismash"
ORIGIN
        1 cctctatcta tgaagccatg gtccgtatgg ctcaatggtg gagttaccgt tacatgcttg
       61 tagatggtca tgggaacttt ggttccatgg atggtgatgg tgctgccgcc cagcgttata
      121 ctgaggcacg tatgagcaag attgctctgg agatgcttcg tgatatcaat aaaaatacgg
      181 ttgatttcgt tgataactat gatgccaatg aacgtgaacc tttagtcctt ccagctcgtt
      241 ttccaaatct tttagttaat ggagctactg gtatcgctgt cggtatggcg accaatattc
      301 cacctcataa cttgggtgaa actattgatg ctgtgaagtt ggtcatggac aatcctgaag
      361 tgaccactaa ggatttgatg gaagtcttgc ctgggccaga ttttccaact ggtgccctgg
      421 ttatggggaa atcaggcatt cataaggctt acgaaactgg taaaggttct attgtccttc
      481 gttcacgcac tgaaattgaa gaaactaaaa ctggccgtga gcgtattgtt gtaacagaat
      541 ttccttatat ggtcaataaa acaaaggtgc atgagcatat tgttcgtttg gttcaggaaa
      601 aacgtattga gggtattact gcagttcgtg atgaatcaaa ccgtgaaggg gttcgtttcg
      661 taatcgaggt caaacgtgat gcctcggcca atgttatcct taataatctc ttcaagatga
      721 cccagatgca aaccaacttt ggtttcaata tgctagctat ccaaaatggt gtaccgaaga
      781 ttttgtctct tcgtcagatt ttggatgctt atattgagca ccaaaaagaa gtggttgttc
      841 gtcgtactcg ttttgataag gaaaaagcag aaacgcgtgc tcatatcttg gaaggtctct
      901 taattgcact agaccatatc gatgaagtga ttcgtatcat ccgtgctagt gaaacggatg
      961 cggaagcaca agctgagttg atgagcaagt ttaagctttc tgaacgtcaa agtcaagcca
     1021 ttcttgatat gcgtcttcgt cgtttgacag gtttggaacg tgataagatt cagtctgagt
     1081 acgacgattt aattgctttg attgctgact taactgatat tcttgctaaa ccagaccgag
     1141 tagcgcaaat catcaaggat gaattggatg aagtcaaacg taagtttgga gataatcgtc
     1201 gtacagagtt gatggtggga gaagtcttga cccttgaaga tgaagatttg attgaagaat
     1261 cggatgtctt aattacactg tctaacaaag gttacatcaa acgtttggat caaggagaat
     1321 tcactgctca aaaacgtggt ggtcgtggtg tccaagggac aggtgttaag gatgacgatt
     1381 ttgttcgtga gttagtgtca actagcaccc atgatcacct actcttcttt acaaataaag
     1441 gacgtgtcta tcgcctgaaa ggctatgaaa ttcctgagta tggtcgtaca gctaagggct
     1501 tgccaatcgt caatcttttg aaattagatg aaggtgaaag tatccagacc atcatcaatg
     1561 ttgagtctga acgtagtgat gatgcttacc tcttcttcac aacccgtcat ggtattgtaa
     1621 agagaaccag cgttaaggaa ttcgcaaaca ttcgtcagaa tggtctcaag gctttgaacc
     1681 tcaaggatga agatgagttg attaatgtat tgttgacgga agaagacacg gatattatca
     1741 ttggtaccaa gtttggttat gcagttcgct ttaatcaatc agccgttcgc ggcatgagtc
     1801 gtattgccac aggtgtgaaa ggtgtgaatc tccgtgaggg agacacagtt gttggtgcta
     1861 gtgtgattac agaccaggac gaggttctta tcatcacaga aaagggctat ggtaagcgca
     1921 cagttgcgac cgaatatcca actaaaggtc gtggtggtaa aggaatgcaa acagctaaga
     1981 ttacagaaaa gaacggttct cttgctggtc ttatgaccgt taaaggtgat gaagatttga
     2041 tgattatcac tgatactggt gtcatgatta gaaccaacgt tgccaatatt tcacaaacag
     2101 gtcgctcaac tatgggagtt aaggtgatgc gcctcgatca agatgccaaa attgtaacct
     2161 ttactacggt tgcagcggca gaaaaagaag aagttgggac agaaaacgaa acagaaggtg
     2221 aagcataatg tctcataaaa agaataataa gaaaatcaaa cgaaaaaatc tgctgacaaa
     2281 tatcttggct gtatttctca tactgatctc tattgcttta atttttaatg caaagattcg
     2341 tgatgcattt attgcttgga atagtaataa ataccaagtt agtcaagtat caaaagaaaa
     2401 aatagaagaa aatcaggata cagagggaaa ttttgatttt gattcagtga aagctatttc
     2461 ctctgaagct gttttagcct ctcaatggga taatcaaaaa ttacctgtta tcggaggaat
     2521 tgcaatacct gagcttgaaa ttaatttacc tatctttaag gggattgcaa atgtcaatct
     2581 tttatatggt gcgggtacca tgaagcgtga ccaagaaatg ggtaaaggga attattcatt
     2641 agctagccat catattttca caggtgataa tgctagtcag atgctctttt ctccactaga
     2701 tcgtgcgaaa aaagggatga aaattcactt gacagataaa aataaagtgt atacctatga
     2761 aattagtgaa gtgaaacgcg ttacaccaga tagagtagat gaaattgatg accgaactgg
     2821 tgttgatgaa attactttag taacttgtga ggatgcagca gctactgagc ggattattgt
     2881 aaagggtgat ttgaaagaaa caaaagatta ttcacaaaca tctgatgaaa ttctgacagc
     2941 cttcaatcaa ccatataaac aattttatta gttctaaaag aatcagtgaa gattaagttt
     3001 cattgattct tttaatattt tcataaaata aacttttatg aaatacagaa aacgtttcct
     3061 taattctaaa gtttatgtta taattatcac aaataaaagt ttaggagttt attatggttt
     3121 cttcagaatt tatctcaaag attgaatttg cttgcaagaa gaaggaaagt ctttatagtc
     3181 aaagcaaatt taagtatgca attcgttcta tgttcgcagg tgcattttta accttcagta
     3241 cggctgcagg tgcagttggg gctgatttga ttaataaaat tgcaccaggt agtggacgat
     3301 tcctctttcc atttgtcttt gcttggggct tggcctacat tgttttcttg aatgctgagt
     3361 tggtaacatc aaacatgatg tttttgactg ctggtagttt cttgaaaaaa atctcttgga
     3421 gaaaaacagc tgagatttta ctttactgta ccttgttcaa ccttattggg gccttgatag
     3481 caggttgggg ctttgctcat tcggcagcct atgcaaatct gacacacgat agtttcatct
     3541 caggtgttgt tgagatgaag ttaggtcgct caaatgaatt aatcttgctt gaggcgattt
     3601 tggcaaatat tttcgtaaat attgcgattc tttcatttgt tttggtcaaa gatggtggtg
     3661 ccaaactttg gcttgtgttg tcagcgattt acatgtttgt atttttaaca aacgagcaca
     3721 ttgcggcgaa ctttgcttct ttcgcgattg taaaattcag tgttgctgct gatactattg
     3781 ctaacttcgg tgttggaaat atgcttcgtc actggggtgt aacctttgtt ggaaacttta
     3841 tcggaggagg cctcttgatg ggtcttccat atgccttcct caataaaaac gaagatactt
     3901 atgtagatta agaaaatgag cacgattgag tcgtgctttt ttcgttttta aaatagagta
     3961 atagctattt cttatatcaa aatatagaaa actaatattg gtaaactata acttaaggtg
     4021 ctacaatatc cttaatcaaa ctatatggag gtcgccttat gactcgtgat tttaaatttg
     4081 aaaccttaca attgcatgct ggtcaagttg tggatccagc tactaagtct cgtgcagtac
     4141 cgatttatca aacaacatct tttgtttttg atgacacgca ggaaggtgcc gatttgtttg
     4201 ccttgagaaa accagggaac atttatactc gtatcaccaa ccctacaaca gctgcctttg
     4261 aagaaagaat cgctgccctt gaaggtggtg ttggagctct tgcaacagca tcaggtatgg
     4321 cggcagtgac ttatacgatt ttggctcttg cccatgctgg tgaccatgta gtggctgctt
     4381 cgactattta cgatggaact ttcaatctct tgaaggaaac ccttcctcgt tatgggatca
     4441 caacaacctt tgtcgatgtg gataatttgg aagaagtgga agcagcaatc aatgacaata
     4501 ccaagcttgt cttgattgaa accttaggga atcccttgat aaacattcct gacttggaaa
     4561 aattagctga gattgcgcat aaacaccaga ttccacttgt ttctgacaat acctttgcta
     4621 caccttattt gattaatgtc ttttctcacg gtgtagatat ttccattcac tctgcgacta
     4681 agtttatcgg tgggcatggt acgactattg gaggagtgat tgtcgatagc ggtcgttttg
     4741 actgggaggc ttcagggaaa ttccctcaat ttgttgagga agacccaagc taccacaatt
     4801 tgagctatac tcgtgatgtg ggtgcagcag cctttattat cgctgttcgt gttcaattgc
     4861 tccgtgatac aggtgcagcc ttgtcgccat ttaatgcctt tctcttgctc caaggacttg
     4921 aaaccctttc tcttcgtgtc gaacgtcatg tgcaaaatgc agagaaaatt gttgatttcc
     4981 ttgtcaacca tcctaaggta gaaaaggtaa actatccaaa acttgcagac agtccttatc
     5041 atgccttggc tgagaaatac ttgccaaaag gtgtgggctc aatctttact ttccatgtca
     5101 aaggtgggga ggcagaagca cgcaaggtaa ttgatcattt agaaatcttc tctgaccttg
     5161 caaatgtggc agatgccaag tctcttgttg ttcacccagc aacaactact cacggtcaat
     5221 tgtcagaaaa agacctggaa gcagctggcg tcacaccaaa ccaaatccgc ttgtctatcg
     5281 gtcttgaaaa tgtagaagat ttgattgaag acttgcgctt ggccttggaa aaaatttaaa
     5341 gaaaaagaag ttaaacggtg ggtttcgact cactgttttt gattttccct caggcatgat
     5401 ataatggtta cagaagccta gaaagaggaa cgatatgaac gaaatcaaat gtcccaactg
     5461 tggagaagtc tttacagtaa atgaaagtca gtatgctgaa cttttatccc aagtgagaac
     5521 agcagagttt gataaggaat tgcacgatcg catgaagcag gaactggcct tggcagagca
     5581 aaaggccatg aatgagcaac agactaaact ggctcaaaaa aaccaagaaa ttgcgcaatt
     5641 acagagtcaa atccaaaact ttgatacaga aaaagaattg gccaagaaag aggttgaaca
     5701 gacaagcaat caggccttat tggctaagga taaggaagta caggccttgg aaaaccaatt
     5761 ggctacctta cgtttggagc atgaaaatca actacaaaag accctttctg acctagaaaa
     5821 agaacgcgat caggtcaaaa atcagcttct tttacaagaa aaagaaaatg agctttctct
     5881 tgcttctgtt acacaaaact atgaggcaca gcttaaagca gctagtgaac aagtcgaatt
     5941 ttataagaat tttaaggctc aacaatccac aaaagccatc ggggaaagtc tggaacacta
     6001 tgctgaaagt gagttcaata aggtccgcag ttttgcattt ccaaatgcct actttgagaa
     6061 ggataacaag gtctctgcgc gtgggtctaa gggggacttt atcttccgtg aatgtgatga
     6121 aaatggggtc gaaatcattt ccattatgtt tgagatgaaa aatgaagcgg atggaacaga
     6181 gaagaagcac aagaatgcag atttttataa ggaattggac aaggaccgtc gggaaaagaa
     6241 ctgtgagtat gccgttttgg tgactatgct tgaggccgat aacgactatt ttaacacggg
     6301 gattgttgat gtcagccacg agtatgaaaa gatgtatgtg gttcgtcctc aattctttat
     6361 ccagttgatt gggctcttgc gaaatgcggc gcttaattcc ttaaaataca agcaagagtt
     6421 ggccttggtt cgcgaacaga atattgacat tacccatttt gaagaagact tggatgcctt
     6481 taagctagct tttgctaaga actataattc agcttcgact aactttggaa aagctattga
     6541 tgaaatcgac aaggccatca aacgcatgga ggaggtcaag aaattcctga ccacatcaga
     6601 aaatcaactc cgtctcgcaa acaataaatt ggaagatgtt tccgtcaaga aattgacccg
     6661 taaaaatcca accatgaaag ctaagtttga agcgctgaag gaggagtaga aagcaagaca
     6721 tgaacggtat tatcaactta agaaaagaag cagggatgac ctcgcatgat gtggttttta
     6781 aactgcgtaa gattttggga accaagaaaa ttggtcatgg tgggactttg gatccggatg
     6841 tagtgggagt tctgcccatt gcggttggca aagcgacacg catggtcgag tttatgcagg
     6901 atgagggcaa ggtctatgag ggtgaaatca ctcttggcta ttccacgacg actgaggatg
     6961 ccagtgggga agtggtcgca gagacacccg ttctgtcgcc cttggatgaa atcattgttg
     7021 atgaagcgat tgccagtttg actgggccta ttacccagat tccgcctatg tattcggctg
     7081 taaaggtcaa tggtcgtaag ctttatgagt atgcgcgttc gggtcaggaa gtagagcgcc
     7141 cagaacgtca agtaaccatt tatcaatttg atcgaacaag tccgatttct tatgaaggcg
     7201 aacttgcacg attcactttt cgtgtgaaat gtagcaaggg aacctatatt cgtaccttat
     7261 cggttgactt gggagaaaaa cttggttatg cggcccatat gtcacatttg acacggacta
     7321 gtgcagctgg tttgcaacta gaagatgctc ttaccttgga agaaattgct gaaaaagtgg
     7381 aggctggtca actggacttt ctccatcctc tagaaattgg gacaggagac ctcgtcaaag
     7441 ttttcctaag tccagaagag gctacagaag tgcgttttgg tcgttttatc gagctcgacc
     7501 aaacagaaca agaattagct gcctttgaag ataataaatt gctagccatt ctagaaaaac
     7561 gaggcaatct ctacaaacca cgaaaggttt ttagctagtc taactggggt gtggggagtg
     7621 ttgtttcccc tagactatcc aaatcagact ataaatttcc ccaaaaatgt gatagaatag
     7681 acgacggata aaaaaacgga ggatagcatg caaaatagac caatcattat cggagtgaca
     7741 ggtggttctg gtggtggtaa aaccagtgtt tcaagagcca ttttatcgca ttttcctgat
     7801 gaaaagattt ccatgattga gcatgattca tactacaagg atcagtctca tttgaccttt
     7861 gaagagcgtg tcaaaaccaa ctacgaccat ccttttgcct ttgatacaga cttgatgatt
     7921 gagcagatta aggaattgtt ggcagggcgt ccggtggaca tcccgactta tgactataca
     7981 gagcatacac gaagtagcaa gacctatcgt caggagcctc aagatgtctt tatcgttgag
     8041 gggattttgg tcttggagga caagcgcctg cgcgatttga tggatatcaa gatttttgtg
     8101 gatacagatg atgatgtgcg tattattcgg cgtatcaagc gtgatatgga ggagcgtggc
     8161 cgtagccttg atagcgttat tgaccagtat ctaggtgtgg tcaaacccat gtaccaccag
     8221 tttatcgagc caaccaagcg ttatgctgat atcgtcattc ctgagggagt cagcaataca
     8281 gttgctatcg acctgttgac gaccaagatt gcaaagattt tggaagaagc tagaaatagt
     8341 aaataaacag aagtggaggc cttgcctcct ttttctattt ttccttcata atggtacata
     8401 aatggagatt tttaggcata tttttggtat aataataccc atgaaagagc aaaaaaatga
     8461 atagtaggtg gagatggaaa agtatttatc ggtaacaact ttgaccaagt atctgaaaat
     8521 gaaattcgat aaagaccctt acttggaacg ggtctattta actggtcaag tttccaactt
     8581 tcgtaaacga cctactcacc aatatttctc cctaaaggat gaccatgcag ttattcaagc
     8641 gaccatctgg tctggaattt atcagaaatt aggcttcgac ctggaagaag ggatgaaaat
     8701 caatgtgatt gggcgtgtac aggtctacga accaagtggt agctactcca tcatcattga
     8761 aaaggcagag cctgatgggg ttggtgcgct tgcgattcag tttgagcaac tcaagaaaaa
     8821 attgacagaa gaaggcctgt ttcaagagag gttcaagcaa cctatgcccc aattttctaa
     8881 gagaataggt gtagtaacca gtcgcagtgg agccgttatt cgagatatta tcacgactgt
     8941 cagcaggcga tttccaggtg tggatattct tctctatccg accaaggttc aaggtgaagg
     9001 ctctgcggag gaaattgctc gaaatattgc gcgtgctaat caacgggatg atttggactt
     9061 gctgattatt ggtcgtggtg gaggttctat tgaggatctc tgggcattta acgaagaaat
     9121 tgtggtacgt gctatttttg agtctcgttt gccagttatt tctagtgtgg ggcatgagac
     9181 ggatgtgacc ttggcagatt ttgtggcaga tagacgggct gcgacgccga ctgcagcggc
     9241 tgaactggca acacctgtga ccaagttgga tttattagct catttgcaaa atcaggaaaa
     9301 acggatggct acagcagtcc gaaatgtcct atctaagaaa caagaagctt tgaaaaaatg
     9361 cagtcagtct gttatcttta gacagccaga gcgcttgtat gatggctatt tgcaacgctt
     9421 ggatcaacta caactgcgct taaaacaaag tttacgtaca cggatttctg ataacaaaca
     9481 agtcgttcaa gcaaggacac atcgactggt tcaattatca cctgttacca aaattcaacg
     9541 ctctcaagac cgtctaggtc agttggacaa gctcctccgt agccaaatgg cgctggttta
     9601 tgatgccaag gttgctgaag tgaagcgact ttcggaagcc ttgttgatgt tggatactag
     9661 ccgaatcgtg gcgcgtggtt atgctattgt caaaaaagaa gagtccgttg tagattcggt
     9721 tgagagtttg aagaaaaaag accaagtgac gcttttgatg cgagatggtc aagtagaatt
     9781 agaggttaaa gatgtcaaaa caaaagaaat ttgaggaaaa tcttgcagaa ctggagacta
     9841 ttgtccaaag tttggaaaat ggtgaaattg ctttggaaga tgcgattgca gcctttcaaa
     9901 agggcatggt cttgtcaaaa gagctccaag cgacgctgga caaggctgaa aagaccttgg
     9961 tcaaggtcat gcaagaagac ggaacagaaa gtgattttga atgaaaaagc aagaaaaatt
    10021 agctcttgtt gagtcggctt tggaagactt ttatggagac cagcagtttg cctctagttt
    10081 gagagagtcg gttctctatt ccattcatgc tggtggcaag cgtattcggc cttttctctt
    10141 gttggaagtt ttggaagcct tgcaggtcgc tattaaacct gctcacgcgc aggtggctgc
    10201 tgccttggaa atgattcata cagggagctt gattcacgat gaccttcctg ctatggatga
    10261 tgatgattat cgtcggggac gcttgacaaa tcacaagaaa ttcggtgaag ctatggccat
    10321 tttggcagga gatgccttgt tcctagatcc ttacgccttg atagcgcagg cagatttgcc
    10381 aagtcagatc aaggtggact tgattgccaa cttatcgctt gcttcaggaa gtttaggcat
    10441 ggtagcaggg caggttttgg acatggaagg ggaacaccag catttgtctt tggaagagct
    10501 ccaaaccatt catgccaata aaactgggaa gttactagct tatcccttcc aagctgcagc
    10561 cattatagct gaattagtac ctgaaatgca ggtgaagctg aaaactgttg gcgaattgat
    10621 tggacttgcc tttcaagtta gagacgatgt gctagatgtg acagctagtt ttgaggaaat
    10681 cggcaagact ccacaaaagg acttgcaggc agagaagtca acctacccag ccttgttagg
    10741 cttggaagag gcaattgcct tttgtaacca aaccttggat caagctaatg caaaattaga
    10801 agaaattgcc cagcaggtcc cttttgatat aggatcgatt gttagtgtag tagaaagttt
    10861 gagaatcaat ggctaaggaa agagtggatg tactagctta taaacagggc ttgtttgaaa
    10921 cgcgagaaca ggcgaagcgc ggcgttatgg ctggtctagt cgtagcagtc ctgaatggag
    10981 aacggtttga caaaccagga gaaaaaatcc cagatgacac cgagctaaaa ctcaagagtg
    11041 aaaaactcaa gtatgttagt cgtggtggct tgaaactgga aaaggccttg caggtctttg
    11101 atttatcagt tgatggagcg accacaattg atatcggtgc ttctactggt ggttttaccg
    11161 atgtcatgtt gcaaaatggt gctgagttgg tctttgcagt cgatgttggt accaatcagt
    11221 tggcttggaa actacgccag gacccgagag ttgtcagcat ggagcagttt aatttccgct
    11281 atgctgaaaa gactgatttc gaaaaggaac cgagctttgc cagtattgat gtgagtttca
    11341 tttctctcag tctgatcctt ccggccttgc atcgagtctt ggcaggtcaa ggtcaggttg
    11401 tggctctagt taagcctcag tttgaagcag gacgtgagca gattgggaaa aatggaatta
    11461 ttcgcgatgc caaggtccat cagaaagtcc ttgaatctgt aacagctatg gcagtagagg
    11521 aaggtttttc agtccttggt ttgaactttt ctcccatcca aggtgggcat ggaaatattg
    11581 aatttttagc gtatttgaaa aaagaagagt cagcaagcaa tcaggttctt gctgagattg
    11641 aagaagtagt agagagggcg catagtcaat ttaaaaatga ataaaaaaga gagacttgaa
    11701 aaaattagac gatttgtgac agattatcaa atcggtacac aagaagaaat cgtagaacat
    11761 ttgaaagaag ctggcattac tgccactcaa gcaacggtat cccgagatat caaagagcta
    11821 ggtattgtca aaattccttt gagagacaac acctatgtct atgaattgcc aaaatcaatc
    11881 gtgaaaagtt tgcaactggc tgaagacaat attgagtcag ctgaattgat ggacaagatg
    11941 atcaatctcc aagttattcc aggaaacaca gcctttgtaa aagctcagtt aatcgagact
    12001 tttgcggata agatttttag ctgtttggct gatgatagtt cgattttagt cattgccaga
    12061 agtgaaagtc tagctgagga aatctttgaa caagtaaaaa attggtaggt ctatatgtta
    12121 cttgaaattt cgataaaaaa ctttgccatt attgaggcta tatctctcaa ttttgaaaag
    12181 gggatgacgg ttctgaccgg tgaaacaggt gcagggaagt cgattatcat tgatgccatg
    12241 aatatgatgt tgggagctcg tgcgacgaca gatgttattc gtcatggtgc gccaaaggca
    12301 gagattgagg ggcttttctc agttgagaat agtcgccttt tgcaggaaat tttcgatgat
    12361 caaggtttgg aaatggggga tgagattatc atccgtcggg aaattctgca aaatggtcgt
    12421 agtatcagcc gtgtcaatgg tcagatggtt aatctgtctg ttttgcgcgc tattgggcaa
    12481 catctggtag atattcatgg tcagcatgac caagaggagt taatgcgtcc ccaactgcat
    12541 attcaaatgt tggatgaatt tggtgataca ggcttttggg ccttgaaaga aacctatcaa
    12601 acgagttttg atgcctatcg aaaaatgcgt aagcaggttc tggaagtcaa gaaaaaccaa
    12661 caggaacaca aggcacgtat tgaaatgttg gaatttcaaa tggcagagat tgaggcggca
    12721 aacttgcagg ctggagaaga cctgtctctc aaccaagagc gtgataaact tcttaatcat
    12781 aaaaatattg cggatacgct gaccaatgcg tacagtatgt tggacaatga agacttttca
    12841 agtctggcca atgttcgttc agccatgaat gacatggaaa gtgtcgaaga gtatgaccct
    12901 gaataccgtg aaatttcaag ctcactgtct gagacctact atgtcttaga agacattagc
    12961 aaacgtttgg aagctattat tgaggacctt gattttgatg gcaatcgcct catgcaggtt
    13021 gagaatcgct tggatctcct tcatactatt acccgcaagt atggtgggac tgtagacgat
    13081 gttttgcttt attttgccaa gattacggaa gaatataatc tcttgacagg caataatctt
    13141 tcatctgagg acatggaagc agagctcaag aaattggaag ttaatcttgt cgatttggca
    13201 ggtcagcttg catctgctcg tcatgacttg gctcagcagc tagaagcaga gattaaacaa
    13261 gagcttcaag atctttatat ggaaaaagcc cagtttcagg ttcgttttag caagggcaaa
    13321 ttcagtcgtg agggcaatga aatggtcgag ttttacattt caaccaaccc tggcgaagac
    13381 tttaaaccct tggtcaaggt tgcatctggc ggtgaattat ctcgtctcat gctagctatt
    13441 aagtctgcct tttcacgtaa agaaggcaag acaagtattg tctttgatga agtggataca
    13501 ggagtctcag gccgtgtagc tcaggccatt gcacagaaga ttcataaaat tggtcagcat
    13561 ggtcaggttt tggctatttc tcatttgcca caagtgattg cgattgcgga ttatcaattc
    13621 tttattgaga agattagcaa tgagcattca acggtttcga ctgttcgtct cttgacggtt
    13681 gaagagcgag tggaggaagt cgctaaaatg ttggcaggtg atgatgtgac ggaagcagcc
    13741 ctgacgcaag ctagagaatt gttgagaaac agggagaaat aagatgacag actattatgt
    13801 aattggagat gttcacggaa aagctgggat gctggaagac cttctcaaaa catgggatgg
    13861 tcagacccag ttgctctttc taggggattt gattgaccgt ggtgaggata gtcgccgtgt
    13921 ccttgaaatg gtcaaggact tggtcgataa tcaaggggca atctgtttgt caggaaacca
    13981 cgagtatatg tttctgactt ggctcgataa cccagaagaa agttatgacc attatcgtcg
    14041 caatggtgga gatacaacca ttaactctat cctaggtcgt cccttggatg caccagttga
    14101 tggggtagaa gataccaagc gggttaccac tgaagcggca gacttggtcg aatttattcg
    14161 tcaaatgccg tttgtggtag agacagataa gtatatcttt gttcacgcag gcattgattt
    14221 gaccttagac gactggcatg aaaccacaga ttacaagaaa gtctggctta gaaaaccatt
    14281 ccacgaagcc gaaaatcata ctgggaaaac tattgtcttt ggtcacacac cagtttatgg
    14341 tttgttgaat caagaccgag gtacagctga gctttggact acagaagatg gcaagattgg
    14401 gatggacgga ggagctgtct atggtggtgt ccttcacggg attgtcttta cagaccaagg
    14461 aatgacagaa caccacttta tcgaaaatga cggctttgtc gctgaagatt agtactccta
    14521 acagggtatg gtcttgtcaa aatgtcaaaa acaatttata ataaatagat accctgaaag
    14581 gaagagaatc atgaacttag aagaattgaa aaaacgacag gagaagatcc gtaacttctc
    14641 tattatcgcc catattgacc acgggaaatc gactcttgca gaccgcattt tggaaaagac
    14701 agagacagta tccagtcgtg aaatgcaagc ccagcttttg gatagcatgg atttagagcg
    14761 agaacgtggg attaccatta aactcaatgc catcgagctg aattatactg caaaagatgg
    14821 ggaaacttat attttccact tgattgacac gccggggcac gtagacttta cctatgaagt
    14881 ttcacgttcg ctagctgcct gtgagggagc gattttggtg gtcgatgccg ctcaaggtat
    14941 tgaggctcaa acacttgcca acgtttatct agccttggac aatgatttgg aaatcatgcc
    15001 agtcattaat aaaattgacc tgccagcagc agaccctgag cgcgtgcgta cagaaattga
    15061 agatgtcatt ggtttggatg ccagcgaagc agtattagca tcagctaaag ctggtattgg
    15121 tatcgaagaa attctcgagc aaatcgtaga aaaagtgcca gctccaacag gtgatgtgac
    15181 ggcacctctt aaggccttga ttttcgactc ggtttacgat gcttaccgtg gggttatcct
    15241 ccaagttcgt gtcatggatg gggttgtcaa acctggtgat aagattcagc tcatgagcaa
    15301 tggcaagacc tttgatgtta cggaagttgg tattttcaca ccaaaagcag ttggtcgtga
    15361 tttccttgcg actggtgacg ttggttatat tgcggcttct atcaagacgg ttcaggatac
    15421 tcgtgtgggt gataccgtta ccttggcaac caatcctgcg gcggaaccat tacatggtta
    15481 caagcagatg aatcctatgg tctttgcggg tctatatcct atcgagtcaa acaagtacaa
    15541 tgacctacgt gaagcccttg aaaaattgca actgaacgat gctagtctcc aatttgaacc
    15601 agaaacatct caggcacttg gatttggttt ccgttgtggt ttccttggac ttctccatat
    15661 ggatgttatc caagagcgtt tggaacgtga gttcaacatt gacctcatca tgacagctcc
    15721 atccgttatc tataaggtta atttgaccga cggcgagtca atggatgtgt ctaacccatc
    15781 tgagttccca gatcctacca agattgcgac cattgaagag ccttatgtta aggcgcaaat
    15841 catggtacca caggagttcg taggagctgt aatggagcta gctcagcgca agcgtgggga
    15901 ctttgtaacg atggactata ttgatgataa tcgtgttaat gttatctatc aaattcctct
    15961 tgctgaaatt gtctttgact tctttgataa gctcaagtct tcaactcgtg gttatgcaag
    16021 ctttgactac gaattgtcag agtatcgccc atctaagctg gtcaaaatgg acatccttct
    16081 caatggagac aaggtggatg ccctcagctt tatcgttcac aaggactttg cctacgaacg
    16141 tggaaaactc atcgttgata aacttaagaa aattatccct cgccaacaat ttgaggttcc
    16201 aattcaagca gctattggac acaagattgt ggctcgtact gatatcaagg cccttcgtaa
    16261 gaacgtactt gctaaatgtt atggtggtga cgtttctcgt aagcgtaaac tccttgaaaa
    16321 acaaaaagct ggtaagaaac gcatgaaagc tatcggatca gtagaagttc cacaagaagc
    16381 cttccttagc gtcttgagta tggatgagga atagaaatga aaacaaaact cttgaagatt
    16441 tctcaagagt ttttgttatt actgttataa tttgacatct atccttattt ttactatagt
    16501 aggtggtagt ggaggaagtt tatgaaaaaa agtttagttt tagcaacaat ttttatatca
    16561 agttttgcat taacggcatg ttcaaataag agtcaaacag aatctagcac gaatgtgatt
    16621 actagttctt caagtagtca ggaagcgtct acctcgacga ctacaagttc tgataagaag
    16681 actgaagaaa cacaggttat tggttctgat gagtatgggt tcgttaaagt gccaaagtct
    16741 tggattaagt ttaatgaagt acagggaggc aatgatattc aatattcaga cggtacggat
    16801 atcaatattg tcaccttgaa tacttttaaa gcagaacaat ttggtatttc tgataatgaa
    16861 tttcaaaaac ttgaaacagt tcgtatttct gatagtattt tcacttcaaa agaaagtaat
    16921 ccagaattca ctaaagtttg gggttctaag tcaactattg gaggttatga agcctatgta
    16981 gttaacgcaa tttctaagtc tggaaaatac atcattacat ggatttttaa atctagtgat
    17041 ggtaagttca gatatgttgc tttagaaggg actccagaaa cattaaaaaa tctacttccc
    17101 atgattgaag agagttggac aattcaaaaa taattattaa agcaggattg gtatcctgtt
    17161 tttttaaaag tcattaaaaa ccgttttaaa tgtttgtttt aaatgtttgt tttgcttata
    17221 aagataaaaa ataaacaaaa caatcaaaaa aaggacaaaa aacaacaaaa aatattgaca
    17281 gcggtttcat atagtgttat acttatagca taaagttaat ggaaagaagg gaaaacctta
    17341 tgggattaga tcatttcttt gacaaaaacc ttgtgttttg cttagaagcg gataatcaag
    17401 aacatctctt tgatcaggtt gcaactttat tggaagaacg agaaatcgta actccaactt
    17461 atcgtgaagc cttgatcacg cgtgaaaagt catttccaac tggcttagat atggaatttt
    17521 tgggtaagga tttgccgaat gtggctattc ctcacacgga tattgctcat aatctagccg
    17581 aaaaagttgt ggtcgttcga ttagaaaaac cagtgacttt ccacaatatg attgcgccag
    17641 ataaggaagt agaagtatcc ttactcttct ttatcattaa taactcaagt tcaagccaaa
    17701 ccaacattct tgctcagttg atggacttct ttactggaaa tggtcatctt gaagacctat
    17761 caaaaatttc tgaaccagaa gccctctatg cttatattgc taaagcaatt gcttaatctt
    17821 gtctattaaa aatattaaat cggaggaaat ccatatgatt aaaattcttg ctgcctgcgg
    17881 tgcaggtgtt aactcaagcc accaaattaa aagtgctctc gaagaagaac tttcaaaccg
    17941 cggttttgat gttcactgtg atgcagttat ggtaaaagac gttaacgaag accttatcaa
    18001 aggttatgac atcttcacac caattgctgc gacagatctt ggttttgacc caggtattcc
    18061 agttatcgaa gctggaccaa tcttattccg tattccagca atgagtgctc cagtatttga
    18121 caatattgaa gcagctatca aagaacacgg attaagctaa ttttaatttg ttaacattca
    18181 tataactata aaaagggagg aactattatg gatgtcatta tcgaactagc caataagctg
    18241 ttcaaaccta tcttggatat gggtggacca atcatcatgc tgatcatttt gacagtattg
    18301 gctctacttt ttggagtgaa attctccaaa gcgcttgaag gtggtatcaa acttgccatc
    18361 gctcttacag gtatcggtgc catcattggt atgttaaatg gtgctttctc agcatcactt
    18421 gcaaaatttg ttgaaaacac tggtattcaa ttgaacatca ccgacgttgg ttgggcacca
    18481 cttgctacaa tcacttgggg atctgcttgg acactttact tcttgctcat catgttgatt
    18541 gtcaacgtag tgatgcttgc tatgaagaaa actgacacac ttgacgttga tatcttcgat
    18601 atctggcact tgtctatcac aggtcttttg attaaatggt atgcagacaa caatggagtt
    18661 agccaaggag tttcactctt catcgcgact gctgcagttg ttcttgttgg ggttcttaaa
    18721 atcatcaact ctgacttgat gaaaccaaca tttgacgatc ttcttaatgc accaagttca
    18781 tcaccaatga cttcaactca catgaactac atgatgaacc cagttatcat ggttttggat
    18841 aagatttttg aaaaattctt cccaggtctt gataaatatg actttgatgc tgctaaattg
    18901 aacaaaaaaa tcggtttctg gggatctaaa ttcttcatcg gtttcatcct tggtatcgtt
    18961 atcggtttga tgggaactcc acatccagtt gaaggactta aagatgcagc aaactggcaa
    19021 gaagttatca aaggttggtt gtctcttggt ttgacagccg gtgtagcttt ggaactcttc
    19081 tcacttatcg gttcatggtt cattgctgcc gtagaaccac tttcacaagg tattacaaac
    19141 gttgctacta aacgtttgga aggacgtaag tttaacatcg gtctagactg gccattcatc
    19201 gctggtcgtg ctgaaatctg ggcttgtgca aacgtacttg caccaattat gttgattgaa
    19261 gctgtgcttc tttcaaacgt cggaaatggt atcttgccac ttgcaggtat catcgctatg
    19321 ggtgttactc cagctctctt ggttgtaaca cgtggtaaat tgctccgtat gattatcttc
    19381 ggaacactct tgttgccact cttccttctt tcaggtacac ttatcgcacc atttgcaaca
    19441 gaacttgcta aaggtgtagg tgccttccca gcaggtgtga gccaaactca attgattact
    19501 cactcaactc ttgaaggacc aatcgaaaaa cttcttggtt ggacaattgg taacactaca
    19561 acaggtgata tcaaagcaat ccttggtgct gtagtcttcc ttgtattcta tatcggtatc
    19621 tttgcttggt acagaaaaca aatgatcaaa cgtaacgaag agtacgcagc aaaagcaaaa
    19681 taatgcgctc ctataaaatg taagctcaat gaaaatcaaa gagcaaacta ggaagctagc
    19741 cgcagcctgt actaaagtac ggcaaggtga cgctgacgaa gtttgaattg gattttcgca
    19801 gagtgtaaat tgttgaaact aggttgtcat accattagga gcgacagcct agttttttat
    19861 aaactattaa gaaatcggag aaaataatgg taattgaatt aaaatcagaa caattaagtg
    19921 ttcaatttaa cagttttggt ggggctcttt cttctataaa agatgctgac ggtcttgagt
    19981 atttgtggca aggggatgct acttattgga gtggacaagc tcctgttctc ttccctattt
    20041 gtggttcatt gagagaggat acagccttct atatagatga gaaagggcaa gaaagtaaag
    20101 gaaagattcc tcgtcatggt ctggttcgta agaaagaatt tgaattagtt gaacaaacgg
    20161 aaaacagtgt aacttttgca attgaagacg atgagaatag ctatcagaac tacccatatc
    20221 atttccgcct agaaatcact tatatcttta ctggaaagac agtacggact caatacaaaa
    20281 tatttaataa agaaactagc aaggtcatgc cttactttat tggtggacat ccaggtttta
    20341 attgtcctct acttgatgat gaagtctatg aagattacta tttagagttt gagaaagaag
    20401 agacttgctc tgttcctcat tcattcccag aaacaggtat gttggatttt caagatagaa
    20461 gtccatggct agaaggtcaa aaagaagtag acctcagcta tgatttgttt agcacagatg
    20521 cagttacctt agatgaattg caatctagaa caattgccct tcgttctcgt aaacatgata
    20581 agggattgaa agtgcacttt gcagagtttc caaatcttat tatctggtca actttgaata
    20641 agggaccttt cattgctttt gaaccatggt ctggcttgtc aacatccctt gaagaaggag
    20701 atcatctaga agataagaaa aacgttcgcc tcctagaaca tggtcaagtg gatcaaattg
    20761 cttttgatat cgaaatcttt tagataaaaa aacacaaaaa caaacatttg ctgttgactt
    20821 tttcgacaaa taagagtata ttatgtttgt aagcaacaaa tgatgtttgt cgcaaa
//
