GCF_023094165.1 - 55 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

NZ_JALMDK010000010.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	terpene	67,645	88,814

NZ_JALMDK010000011.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 2.1	terpene	206,979	227,908
Region 2.2	hglE-KS,azole-containing-RiPP,RRE-containing	243,420	299,141

NZ_JALMDK010000013.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 4.1	terpene	195,143	216,054

NZ_JALMDK010000014.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 5.1	RRE-containing	80,609	101,715

NZ_JALMDK010000015.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 6.1	CDPS	52,556	73,266
Region 6.2	oligosaccharide	97,333	120,753

NZ_JALMDK010000016.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 7.1	NRPS,ectoine,lanthipeptide-class-iv	1	95,421
Region 7.2	indole,terpene	116,595	144,886

NZ_JALMDK010000017.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 8.1	lanthipeptide-class-ii	30,251	72,720	Medium	SRO15-3108	ribosomal:RiPP:Lanthipeptide
Region 8.2	arylpolyene,lanthipeptide-class-iii,terpene	94,554	171,683	Medium	pactamycin	PKS
Region 8.3	NRPS,T1PKS	180,512	230,412

NZ_JALMDK010000018.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 9.1	T1PKS,NRPS-like,NRPS	137,264	223,194	Medium	rotihibin A	NRPS:Type I

NZ_JALMDK010000019.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 10.1	NRPS	23,971	87,000	Low	thiazostatin/watasemycin A/watasemycin B/2-hydroxyphenylthiazoline enantiopyochelin/isopyochelin	NRPS:Type I
Region 10.2	T1PKS	153,661	202,948

NZ_JALMDK010000001.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 11.1	butyrolactone	419,356	430,255
Region 11.2	HR-T2PKS	677,811	719,969
Region 11.3	redox-cofactor	869,302	891,410	Low	lankacidin C	NRPS:Type I+PKS
Region 11.4	T1PKS	1,048,811	1,095,332
Region 11.5	terpene	1,125,863	1,152,638	High	2-methylisoborneol	terpene:Monoterpene
Region 11.6	terpene	1,164,665	1,185,939

NZ_JALMDK010000020.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 12.1	T1PKS,NRPS-like	55,545	130,535	Low	niphimycin C/niphimycin D/niphimycin E/17-O-methylniphimycin/niphimycin Iα/19-O-malonylniphimycin	PKS

NZ_JALMDK010000021.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 13.1	lanthipeptide-class-iii	93,534	116,092

NZ_JALMDK010000023.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 15.1	NRPS,NRP-metallophore	22,958	99,518

NZ_JALMDK010000025.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 17.1	lassopeptide	40,517	62,816

NZ_JALMDK010000027.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 19.1	NAPAA,lanthipeptide-class-ii	26,063	62,048	High	ε-Poly-L-lysine	NRPS:Type I

NZ_JALMDK010000002.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 22.1	azole-containing-RiPP	74,384	96,722	Medium	streptamidine	ribosomal:unmodified
Region 22.2	CDPS	638,957	659,652
Region 22.3	lanthipeptide-class-i	743,846	770,333

NZ_JALMDK010000031.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 24.1	T1PKS	1	22,999

NZ_JALMDK010000003.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 33.1	terpene	26,837	49,218	Low	peucechelin	NRPS:Type I
Region 33.2	terpene-precursor	197,322	218,428	Low	isorenieratene	terpene
Region 33.3	lanthipeptide-class-i	608,197	632,751
Region 33.4	terpene	820,734	841,789
Region 33.5	other,RRE-containing	855,885	897,234	Low	phosphoramidon	other:other
Region 33.6	sactipeptide	919,260	940,774
Region 33.7	terpene	949,853	973,866

NZ_JALMDK010000004.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 36.1	lassopeptide	62,366	84,932
Region 36.2	hydrogen-cyanide,betalactone	192,137	227,445
Region 36.3	indole	289,053	310,150
Region 36.4	RRE-containing	451,469	472,575
Region 36.5	NI-siderophore	594,774	626,399	High	branched-chain fatty acids	other:other
Region 36.6	terpene	642,012	663,055
Region 36.7	furan	814,281	835,279

NZ_JALMDK010000005.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 37.1	terpene-precursor	58,193	79,284
Region 37.2	RiPP-like	396,490	407,305
Region 37.3	T1PKS,NRPS	488,960	645,687	Low	carrimycin	PKS

NZ_JALMDK010000006.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 38.1	T1PKS,PKS-like,T2PKS,lanthipeptide-class-iii	600,793	698,907	Medium	hedamycin	PKS

NZ_JALMDK010000007.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 39.1	NRPS	58,774	126,307
Region 39.2	lanthipeptide-class-v	224,199	278,366	High	branched-chain fatty acids	other:other
Region 39.3	lanthipeptide-class-i	358,658	383,003
Region 39.4	T1PKS	393,564	539,781	Medium	nystatin	PKS

NZ_JALMDK010000008.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 40.1	lassopeptide	26,026	48,829
Region 40.2	lanthipeptide-class-v	303,862	351,358

NZ_JALMDK010000009.1 (Crossiella sp. S99.2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 41.1	lassopeptide	308,283	330,858

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	terpene	67,645	88,814
Region 2.1	terpene	206,979	227,908
Region 2.2	hglE-KS,azole-containing-RiPP,RRE-containing	243,420	299,141
Region 4.1	terpene	195,143	216,054
Region 5.1	RRE-containing	80,609	101,715
Region 6.1	CDPS	52,556	73,266
Region 6.2	oligosaccharide	97,333	120,753
Region 7.1	NRPS,ectoine,lanthipeptide-class-iv	1	95,421
Region 7.2	indole,terpene	116,595	144,886
Region 8.1	lanthipeptide-class-ii	30,251	72,720	Medium	SRO15-3108	ribosomal:RiPP:Lanthipeptide
Region 8.2	arylpolyene,lanthipeptide-class-iii,terpene	94,554	171,683	Medium	pactamycin	PKS
Region 8.3	NRPS,T1PKS	180,512	230,412
Region 9.1	T1PKS,NRPS-like,NRPS	137,264	223,194	Medium	rotihibin A	NRPS:Type I
Region 10.1	NRPS	23,971	87,000	Low	thiazostatin/watasemycin A/watasemycin B/2-hydroxyphenylthiazoline enantiopyochelin/isopyochelin	NRPS:Type I
Region 10.2	T1PKS	153,661	202,948
Region 11.1	butyrolactone	419,356	430,255
Region 11.2	HR-T2PKS	677,811	719,969
Region 11.3	redox-cofactor	869,302	891,410	Low	lankacidin C	NRPS:Type I+PKS
Region 11.4	T1PKS	1,048,811	1,095,332
Region 11.5	terpene	1,125,863	1,152,638	High	2-methylisoborneol	terpene:Monoterpene
Region 11.6	terpene	1,164,665	1,185,939
Region 12.1	T1PKS,NRPS-like	55,545	130,535	Low	niphimycin C/niphimycin D/niphimycin E/17-O-methylniphimycin/niphimycin Iα/19-O-malonylniphimycin	PKS
Region 13.1	lanthipeptide-class-iii	93,534	116,092
Region 15.1	NRPS,NRP-metallophore	22,958	99,518
Region 17.1	lassopeptide	40,517	62,816
Region 19.1	NAPAA,lanthipeptide-class-ii	26,063	62,048	High	ε-Poly-L-lysine	NRPS:Type I
Region 22.1	azole-containing-RiPP	74,384	96,722	Medium	streptamidine	ribosomal:unmodified
Region 22.2	CDPS	638,957	659,652
Region 22.3	lanthipeptide-class-i	743,846	770,333
Region 24.1	T1PKS	1	22,999
Region 33.1	terpene	26,837	49,218	Low	peucechelin	NRPS:Type I
Region 33.2	terpene-precursor	197,322	218,428	Low	isorenieratene	terpene
Region 33.3	lanthipeptide-class-i	608,197	632,751
Region 33.4	terpene	820,734	841,789
Region 33.5	other,RRE-containing	855,885	897,234	Low	phosphoramidon	other:other
Region 33.6	sactipeptide	919,260	940,774
Region 33.7	terpene	949,853	973,866
Region 36.1	lassopeptide	62,366	84,932
Region 36.2	hydrogen-cyanide,betalactone	192,137	227,445
Region 36.3	indole	289,053	310,150
Region 36.4	RRE-containing	451,469	472,575
Region 36.5	NI-siderophore	594,774	626,399	High	branched-chain fatty acids	other:other
Region 36.6	terpene	642,012	663,055
Region 36.7	furan	814,281	835,279
Region 37.1	terpene-precursor	58,193	79,284
Region 37.2	RiPP-like	396,490	407,305
Region 37.3	T1PKS,NRPS	488,960	645,687	Low	carrimycin	PKS
Region 38.1	T1PKS,PKS-like,T2PKS,lanthipeptide-class-iii	600,793	698,907	Medium	hedamycin	PKS
Region 39.1	NRPS	58,774	126,307
Region 39.2	lanthipeptide-class-v	224,199	278,366	High	branched-chain fatty acids	other:other
Region 39.3	lanthipeptide-class-i	358,658	383,003
Region 39.4	T1PKS	393,564	539,781	Medium	nystatin	PKS
Region 40.1	lassopeptide	26,026	48,829
Region 40.2	lanthipeptide-class-v	303,862	351,358
Region 41.1	lassopeptide	308,283	330,858

No secondary metabolite regions were found in these records:: NZ_JALMDK010000012.1 (Crossiella sp. S99.2); NZ_JALMDK010000022.1 (Crossiella sp. S99.2); NZ_JALMDK010000024.1 (Crossiella sp. S99.2); NZ_JALMDK010000026.1 (Crossiella sp. S99.2); NZ_JALMDK010000028.1 (Crossiella sp. S99.2); NZ_JALMDK010000029.1 (Crossiella sp. S99.2); NZ_JALMDK010000030.1 (Crossiella sp. S99.2); NZ_JALMDK010000032.1 (Crossiella sp. S99.2); NZ_JALMDK010000033.1 (Crossiella sp. S99.2); NZ_JALMDK010000034.1 (Crossiella sp. S99.2); NZ_JALMDK010000035.1 (Crossiella sp. S99.2); NZ_JALMDK010000036.1 (Crossiella sp. S99.2); NZ_JALMDK010000037.1 (Crossiella sp. S99.2); NZ_JALMDK010000038.1 (Crossiella sp. S99.2); NZ_JALMDK010000039.1 (Crossiella sp. S99.2); NZ_JALMDK010000040.1 (Crossiella sp. S99.2); NZ_JALMDK010000041.1 (Crossiella sp. S99.2)

Compact view

NZ_JALMDK010000010.1 - Region 1 - terpene

Download region GenBank file

Download region SVG

Location: 67,645 - 88,814 nt. (total: 21,170 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.46	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002306.2	0.42	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0002318.2	0.38	ribosomal	canucin A, canucin B	Streptomyces canus
BGC0000503.5	0.38	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002316.2	0.37	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0002346.3	0.36	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000516.4	0.36	ribosomal	geobacillin II	Geobacillus thermodenitrificans
BGC0002575.2	0.35	ribosomal	cochonodin I	Streptococcus suis
BGC0000527.5	0.35	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0001787.3	0.35	ribosomal	suicin 3908	Streptococcus suis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.46	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002306.2	0.42	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0002318.2	0.38	ribosomal	canucin A, canucin B	Streptomyces canus
BGC0000503.5	0.38	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002316.2	0.37	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0002346.3	0.36	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000516.4	0.36	ribosomal	geobacillin II	Geobacillus thermodenitrificans
BGC0002575.2	0.35	ribosomal	cochonodin I	Streptococcus suis
BGC0000527.5	0.35	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0001787.3	0.35	ribosomal	suicin 3908	Streptococcus suis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
tetraterpene	C40 carotenoid		C40	unknown	unknown

Genes with terpene domains:

MZC64_RS32945: Lycopene_cycl

Domain 1 (5 - 376): Lycopene cyclase

Reactions:: C40_carotenoid → C40_carotenoid

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (6):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.47 out of 26.33

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.20 out of 30.11

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.94 out of 36.54

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.86 out of 29.25

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.00 out of 25.88

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.20 out of 30.22

Confidence: weak

Terpene

TFBS Finder

Glossary

No acronyms to display

TFBS definitions

Regulator	Description

NZ_JALMDK010000011.1 - Region 1 - terpene

Download region GenBank file

Download region SVG

Location: 206,979 - 227,908 nt. (total: 20,930 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Translocases: transporter

MZC64_RS35065: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002285.2	0.44	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0000650.3	0.42	terpene	carotenoid	Algoriphagus sp. KK10202C
BGC0001456.3	0.41	terpene	isorenieratene	Streptomyces argillaceus
BGC0000641.3	0.40	terpene	carotenoid	Enterobacteriaceae bacterium DC416
BGC0000664.5	0.40	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000639.3	0.40	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000640.3	0.40	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0001227.3	0.39	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000633.3	0.39	terpene	carotenoid	Streptomyces avermitilis
BGC0001364.3	0.38	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002285.2	0.44	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0000650.3	0.42	terpene	carotenoid	Algoriphagus sp. KK10202C
BGC0001456.3	0.41	terpene	isorenieratene	Streptomyces argillaceus
BGC0000641.3	0.40	terpene	carotenoid	Enterobacteriaceae bacterium DC416
BGC0000664.5	0.40	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000639.3	0.40	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000640.3	0.40	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0001227.3	0.39	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000633.3	0.39	terpene	carotenoid	Streptomyces avermitilis
BGC0001364.3	0.38	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
triterpene	C30 carotenoid	diapophytoene	C30	acyclic	none
triterpene		PSPP	C30	acyclic	PP
triterpene		squalene	C30	acyclic	none
tetraterpene	C40 carotenoid	phytoene	C40	acyclic	none
triterpene		hydroxysqualene	C30	acyclic	OH

Genes with terpene domains:

MZC64_RS35075: PT_phytoene_like

Domain 1 (16 - 297): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → diapophytoene; FPP → PSPP; FPP → squalene; GGPP → phytoene; PSPP → hydroxysqualene

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.19 out of 20.47

Confidence: strong

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 25.44 out of 30.22

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.13 out of 19.26

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 27.13 out of 30.11

Confidence: strong

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 18.86 out of 25.31

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 25.66 out of 30.11

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 23.67 out of 30.11

Confidence: medium

Additional weak hits (7):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.04 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.80 out of 29.38

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.41 out of 26.33

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.11 out of 28.19

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.64 out of 23.43

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 22.56 out of 36.53

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
PP	Diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
CatR	H2O2-responsive repressor
CelR	Cellobiose uptake repressor
ClgR	Caseinolytic protease gene regulator
VqsM	Regulator of quorum-sensing signalling systems

NZ_JALMDK010000011.1 - Region 2 - RRE-containing,azole-containing-RiPP,hglE-KS

Download region GenBank file

Download region SVG

Location: 243,420 - 299,141 nt. (total: 55,722 nt) Show pHMM detection rules used

hglE-KS: (hglE or hglD)
azole-containing-RiPP: ((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)
RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Azole RiPPs

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

MZC64_RS35210: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

Transferases: glycosyltransferase methyltransferase

MZC64_RS35235: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

MZC64_RS35340: methyltransferase

smcogs: SMCOG1089: methyltransferase

Hydrolases: hydrolase

MZC64_RS35230: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

MZC64_RS49195: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Ligases: synthase

MZC64_RS35215: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

MZC64_RS35220: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001174.4	0.81	ribosomal	corynazolicin	Corynebacterium urealyticum DSM 7111
BGC0001173.5	0.70	ribosomal	plantazolicin	Bacillus pumilus ATCC 7061
BGC0000569.5	0.66	ribosomal	plantazolicin	Bacillus velezensis FZB42
BGC0002013.4	0.49	ribosomal	curacozole	Streptomyces curacoi
BGC0000563.4	0.41	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0001226.4	0.41	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0001146.5	0.40	ribosomal	cyclothiazomycin C	Streptomyces sp. NRRL WC-3908
BGC0000605.5	0.39	ribosomal	GE37468	Streptomyces sp. ATCC 55365
BGC0002346.3	0.39	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0002316.2	0.38	ribosomal	cebulantin	Saccharopolyspora cebuensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002154.2	0.62	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001174.4	0.61	ribosomal	corynazolicin	Corynebacterium urealyticum DSM 7111
BGC0001164.3	0.60	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.60	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001273.3	0.60	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.59	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.59	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.59	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002192.3	0.59	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.59	PKS	ACT-Toxin II	Alternaria alternata

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.26 out of 19.26

Confidence: medium

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 23.74 out of 27.04

Confidence: medium

Additional weak hits (10):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.28 out of 26.33

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.89 out of 23.43

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.98 out of 27.78

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.42 out of 31.73

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.50 out of 30.22

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.24 out of 29.38

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 23.57 out of 33.73

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.97 out of 24.78

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.39 out of 26.85

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.85 out of 28.19

Confidence: weak

(Thi)azol(in)e containing peptide predictions

No core peptides found.

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

RREFinder

TFBS Finder

Azole RiPPs

Predicted core structure(s)

For candidate cluster 2, location 243419 - 299141:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (mal)
Non-colinear ordering:: MZC64_RS35215 + MZC64_RS35220 + MZC64_RS35225

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 243419 - 294785:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (mal)
Non-colinear ordering:: MZC64_RS35215 + MZC64_RS35220 + MZC64_RS35225

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MZC64_RS35215: mal

PKS_AT (1209..1500): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 58.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 66.4
Methoxymalonyl-CoA: 21.5
inactive: 21.1
Methylmalonyl-CoA: 16.6
Propionyl-CoA: 1.6
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

MZC64_RS35220: mal

PKS_AT (609..819): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 3.8
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

RRE predictions

: RRE type: Plantazolicin_C_RRE; Location: 14 - 94; Score: 25.2
: RRE type: Plantazolicin_C_RRE; Location: 14 - 94; Score: 25.2

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
DasR	N-acetylglucosamine dependent repressor

Core peptide predictions

No core peptides found.

NZ_JALMDK010000013.1 - Region 1 - terpene

Download region GenBank file

Download region SVG

Location: 195,143 - 216,054 nt. (total: 20,912 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

MZC64_RS37675: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

MZC64_RS37680: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

Hydrolases: hydrolase

MZC64_RS37645: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000941.3	0.42	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000677.5	0.42	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001364.3	0.41	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919
BGC0002449.2	0.41	other (other)	sarasinoside	uncultured bacterium
BGC0002554.2	0.41	other (other)	carquinostatin A, carquinostatin B	Streptomyces exfoliatus
BGC0001456.3	0.41	terpene	isorenieratene	Streptomyces argillaceus
BGC0001294.5	0.41	other (other)	7-prenylisatin	Streptomyces sp. MBT28
BGC0000633.3	0.40	terpene	carotenoid	Streptomyces avermitilis
BGC0000189.4	0.40	PKS	3-(2'-hydroxy-3'-oxo-4'-methylpentyl)-indole, 3-(2'-hydroxy-3'-oxo-4'-methylhexyl)-indole, 3-(2'-acetoxy-3'-oxo-4'-methylpentyl)-indole, 3-(2'-acetoxy-3'-oxo-4'-methylhexyl)-indole	Xenorhabdus bovienii SS-2004
BGC0001605.3	0.39	terpene	gibberellin	Sinorhizobium fredii NGR234

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000941.3	0.42	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000677.5	0.42	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001364.3	0.41	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919
BGC0002449.2	0.41	other (other)	sarasinoside	uncultured bacterium
BGC0002554.2	0.41	other (other)	carquinostatin A, carquinostatin B	Streptomyces exfoliatus
BGC0001456.3	0.41	terpene	isorenieratene	Streptomyces argillaceus
BGC0001294.5	0.41	other (other)	7-prenylisatin	Streptomyces sp. MBT28
BGC0000633.3	0.40	terpene	carotenoid	Streptomyces avermitilis
BGC0000189.4	0.40	PKS	3-(2'-hydroxy-3'-oxo-4'-methylpentyl)-indole, 3-(2'-hydroxy-3'-oxo-4'-methylhexyl)-indole, 3-(2'-acetoxy-3'-oxo-4'-methylpentyl)-indole, 3-(2'-acetoxy-3'-oxo-4'-methylhexyl)-indole	Xenorhabdus bovienii SS-2004
BGC0001605.3	0.39	terpene	gibberellin	Sinorhizobium fredii NGR234

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
triterpene	C30 carotenoid	diapophytoene	C30	acyclic	none
triterpene		PSPP	C30	acyclic	PP
triterpene		squalene	C30	acyclic	none
tetraterpene	C40 carotenoid	phytoene	C40	acyclic	none
triterpene		hydroxysqualene	C30	acyclic	OH

Genes with terpene domains:

MZC64_RS37665: PT_phytoene_like

Domain 1 (18 - 293): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → diapophytoene; FPP → PSPP; FPP → squalene; GGPP → phytoene; PSPP → hydroxysqualene

Transcription Factor Binding Sites

Binding sites found:

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 27.85 out of 36.53

Confidence: medium

Additional weak hits (12):

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 18.72 out of 35.63

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.37 out of 35.63

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 23.23 out of 36.53

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.80 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.64 out of 28.14

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 18.12 out of 26.12

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 23.17 out of 36.54

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.74 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.76 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.33 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.10 out of 26.06

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.21 out of 28.19

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
PP	Diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description
DmdR1	Iron(II)-dependent repressor

NZ_JALMDK010000014.1 - Region 1 - RRE-containing

Download region GenBank file

Download region SVG

Location: 80,609 - 101,715 nt. (total: 21,107 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002064.2	0.26	ribosomal	felipeptin A2, felipeptin A1	Amycolatopsis sp. YIM 10
BGC0001366.5	0.24	PKS	BE-24566B, zunyimycin A	Streptomyces sp. FJS31-2
BGC0001513.3	0.23	PKS	anthrabenzoxocinone	Actinobacteria bacterium
BGC0000821.5	0.21	other (other)	rebeccamycin	Lechevalieria aerocolonigenes
BGC0000877.5	0.21	other (other)	polyoxin A, polyoxin H	Streptomyces cacaoi subsp. asoensis
BGC0000197.5	0.21	PKS, saccharide	aranciamycin	Streptomyces echinatus
BGC0000395.5	0.20	NRPS	nocardicin A	Nocardia uniformis subsp. tsuyamanensis
BGC0002443.3	0.20	PKS	mycemycin C, mycemycin A, mycemycin B	Streptomyces olivaceus
BGC0001801.5	0.19	NRPS	thiazostatin, watasemycin A, watasemycin B, 2-hydroxyphenylthiazoline enantiopyochelin, isopyochelin	Streptomyces venezuelae ATCC 10712
BGC0002130.3	0.19	ribosomal	cacaoidin	Streptomyces cacaoi CA-170360

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002064.2	0.26	ribosomal	felipeptin A2, felipeptin A1	Amycolatopsis sp. YIM 10
BGC0001366.5	0.24	PKS	BE-24566B, zunyimycin A	Streptomyces sp. FJS31-2
BGC0001513.3	0.23	PKS	anthrabenzoxocinone	Actinobacteria bacterium
BGC0000821.5	0.21	other (other)	rebeccamycin	Lechevalieria aerocolonigenes
BGC0000877.5	0.21	other (other)	polyoxin A, polyoxin H	Streptomyces cacaoi subsp. asoensis
BGC0000197.5	0.21	PKS, saccharide	aranciamycin	Streptomyces echinatus
BGC0000395.5	0.20	NRPS	nocardicin A	Nocardia uniformis subsp. tsuyamanensis
BGC0002443.3	0.20	PKS	mycemycin C, mycemycin A, mycemycin B	Streptomyces olivaceus
BGC0001801.5	0.19	NRPS	thiazostatin, watasemycin A, watasemycin B, 2-hydroxyphenylthiazoline enantiopyochelin, isopyochelin	Streptomyces venezuelae ATCC 10712
BGC0002130.3	0.19	ribosomal	cacaoidin	Streptomyces cacaoi CA-170360

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.65 out of 18.46

Confidence: strong

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 21.64 out of 26.33

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 18.33 out of 19.52

Confidence: strong

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.11 out of 19.52

Confidence: medium

Additional weak hits (5):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.40 out of 39.62

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 22.44 out of 36.53

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 22.90 out of 30.98

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.08 out of 26.85

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.92 out of 28.19

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: Pantocin_Microcin_RRE; Location: 0 - 93; Score: 49.4

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
RpoN	Regulator of nitrogen assimilation and virulence

NZ_JALMDK010000015.1 - Region 1 - CDPS

Download region GenBank file

Download region SVG

Location: 52,556 - 73,266 nt. (total: 20,711 nt) Show pHMM detection rules used

CDPS: CDPS

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Isomerases: mutase

MZC64_RS39420: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000948.5	0.46	other (other)	α-galactosylceramide	Bacteroides fragilis NCTC 9343
BGC0002103.2	0.45	other (other)	pulcherriminic acid	Bacillus subtilis subsp. subtilis str. 168
BGC0001817.5	0.42	other (other)	vancosamine	Streptomyces sp. WAC2288
BGC0000650.3	0.37	terpene	carotenoid	Algoriphagus sp. KK10202C
BGC0000844.5	0.36	other (other)	clavulanic acid	Streptomyces clavuligerus
BGC0002436.2	0.34	other (other)	coformycin	Streptomyces citricolor
BGC0000214.5	0.31	PKS	cosmomycin B	Streptomyces olindensis
BGC0000192.3	0.29	PKS	aclacinomycin	Streptomyces galilaeus
BGC0000826.5	0.29	other (other)	staurosporine	Streptomyces clavuligerus ATCC 27064
BGC0000825.5	0.29	other (other)	staurosporine	Streptomyces sp. TP-A0274

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000948.5	0.46	other (other)	α-galactosylceramide	Bacteroides fragilis NCTC 9343
BGC0002103.2	0.45	other (other)	pulcherriminic acid	Bacillus subtilis subsp. subtilis str. 168
BGC0001817.5	0.42	other (other)	vancosamine	Streptomyces sp. WAC2288
BGC0000650.3	0.37	terpene	carotenoid	Algoriphagus sp. KK10202C
BGC0000844.5	0.36	other (other)	clavulanic acid	Streptomyces clavuligerus
BGC0002436.2	0.34	other (other)	coformycin	Streptomyces citricolor
BGC0000214.5	0.31	PKS	cosmomycin B	Streptomyces olindensis
BGC0000192.3	0.29	PKS	aclacinomycin	Streptomyces galilaeus
BGC0000826.5	0.29	other (other)	staurosporine	Streptomyces clavuligerus ATCC 27064
BGC0000825.5	0.29	other (other)	staurosporine	Streptomyces sp. TP-A0274

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.19 out of 20.47

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.39 out of 19.26

Confidence: strong

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 21.45 out of 25.31

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 22.52 out of 29.72

Confidence: medium

Additional weak hits (4):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.64 out of 21.84

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.69 out of 20.97

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.15 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.14 out of 28.19

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
CatR	H2O2-responsive repressor
NtrC	Nitrogen regulatory protein C
VqsM	Regulator of quorum-sensing signalling systems

NZ_JALMDK010000015.1 - Region 2 - oligosaccharide

Download region GenBank file

Download region SVG

Location: 97,333 - 120,753 nt. (total: 23,421 nt) Show pHMM detection rules used

oligosaccharide: (repeatable(3, [DUF1205, Glyco_transf_28, Glycos_transf_1, Glycos_transf_2, MGT]) and minscore(MGT, 150))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: aminotransferase glycosyltransferase

MZC64_RS39595: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

MZC64_RS39600: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

MZC64_RS39605: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

MZC64_RS39655: aminotransferase

smcogs: SMCOG1019: aminotransferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000277.5	0.52	PKS	urdamycin	Streptomyces fradiae
BGC0002458.3	0.47	NRPS	alazopeptin	Streptacidiphilus griseoplanus
BGC0000587.5	0.45	ribosomal	microcin H47	Escherichia coli
BGC0002573.2	0.43	other (other)	phosphoramidon	Streptomyces mozunensis
BGC0001496.3	0.43	ribosomal	actinokineosin	Actinokineospora spheciospongiae
BGC0002306.2	0.42	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0002700.2	0.42	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0001210.5	0.41	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0001552.3	0.41	ribosomal	citrulassin F	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000513.3	0.41	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000277.5	0.52	PKS	urdamycin	Streptomyces fradiae
BGC0002458.3	0.47	NRPS	alazopeptin	Streptacidiphilus griseoplanus
BGC0000587.5	0.45	ribosomal	microcin H47	Escherichia coli
BGC0002573.2	0.43	other (other)	phosphoramidon	Streptomyces mozunensis
BGC0001496.3	0.43	ribosomal	actinokineosin	Actinokineospora spheciospongiae
BGC0002306.2	0.42	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0002700.2	0.42	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0001210.5	0.41	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0001552.3	0.41	ribosomal	citrulassin F	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000513.3	0.41	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 20.64 out of 23.79

Confidence: strong

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.37 out of 20.47

Confidence: medium

Additional weak hits (7):

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.46 out of 25.75

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.74 out of 30.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 18.59 out of 26.06

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.86 out of 29.25

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.31 out of 20.97

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.32 out of 30.98

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.48 out of 29.25

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
VqsM	Regulator of quorum-sensing signalling systems

NZ_JALMDK010000016.1 - Region 1 - NRPS,ectoine,lanthipeptide-class-iv

Download region GenBank file

Download region SVG

Location: 1 - 95,421 nt. (total: 95,421 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
ectoine: ectoine_synt
lanthipeptide-class-iv: (cds(LANC_like and Pkinase) and not micKC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

MZC64_RS40275: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

MZC64_RS40375: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

MZC64_RS40600: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

MZC64_RS40630: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acetyltransferase aminotransferase methyltransferase

MZC64_RS40350: methyltransferase

smcogs: SMCOG1089: methyltransferase

MZC64_RS40445: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

MZC64_RS40500: aminotransferase

smcogs: SMCOG1019: aminotransferase

MZC64_RS40505: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

MZC64_RS40645: methyltransferase

smcogs: SMCOG1248: methyltransferase

Hydrolases: esterase hydrolase protease

MZC64_RS40285: isochorismatase

smcogs: SMCOG1027: isochorismatase

MZC64_RS40315: enterobactin/ferric enterobactin esterase

smcogs: SMCOG1098: enterobactin/ferric enterobactin esterase

MZC64_RS40540: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Ligases: ligase synthase

MZC64_RS40280: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

MZC64_RS40310: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

MZC64_RS40320: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

MZC64_RS40435: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

MZC64_RS40455: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

MZC64_RS40475: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

MZC64_RS40480: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

MZC64_RS40610: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

MZC64_RS48980: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	1.24	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	1.23	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	1.22	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	1.21	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	1.21	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	1.20	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001261.3	1.20	NRPS	AM-toxin	Alternaria alternata
BGC0002286.3	1.19	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	1.18	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001166.5	1.17	NRPS	HC-toxin	Alternaria jesenskae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002075.3	0.73	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001132.4	0.71	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.71	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.70	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.69	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.69	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001641.4	0.67	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.67 out of 19.26

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 32.19 out of 36.53

Confidence: strong

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.22 out of 18.46

Confidence: strong

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 30.90 out of 36.53

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.23 out of 19.52

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 25.16 out of 30.11

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 23.79 out of 30.11

Confidence: medium

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 26.50 out of 30.12

Confidence: medium

Additional weak hits (30):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.74 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.16 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.05 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.23 out of 28.14

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.40 out of 21.84

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.78 out of 32.74

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.13 out of 30.97

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.25 out of 30.25

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.24 out of 30.97

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.47 out of 26.33

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 24.40 out of 36.53

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 26.02 out of 36.53

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.46 out of 27.55

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.70 out of 36.54

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.93 out of 39.62

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 21.56 out of 36.53

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 26.02 out of 36.53

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 19.20 out of 26.12

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.06 out of 33.73

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.75 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.75 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.57 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.23 out of 29.38

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.57 out of 29.25

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.80 out of 36.54

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.46 out of 29.38

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.79 out of 22.40

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.60 out of 21.84

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.79 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.34 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptides

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Core peptide predictions

No core peptides found.

Predicted core structure(s)

For candidate cluster 1, location 0 - 95421:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Thr) + (Ile) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 36463:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 25998 - 95421:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ile) + (D-Thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MZC64_RS40280: diOH-Bz

Search NORINE for peptide: strict or relaxed

AMP-binding (25..424): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: SASLYPLSSPGGGVQVGGAQQVFGMAEGLVNYTR
Nearest Stachelhaus code(s):: diOH-Bz PLPAQGVVNK (91% 8Å match)
Stachelhaus code match:: 90% (moderate)

MZC64_RS40310: X

AMP-binding (735..1129): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LTQAFDAAFWQGGLVVGGEVNAYGPTETTVNATL
Nearest Stachelhaus code(s):: Fo-OH-Orn DLWGMGAVNK (71% 8Å match)
Stachelhaus code match:: 80% (moderate)

MZC64_RS40320: Lys

Search NORINE for peptide: strict or relaxed

AMP-binding (449..721): Lys

nrpys: Lys

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAAAFDASVEELDLIIGGEVNSYGPTETTVVATT
Nearest Stachelhaus code(s):: Lys DAEDIGSVVK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

MZC64_RS40435: X

AMP-binding (10..414): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VWQAFDISYLEAFIFAGGELNHWGTVEVFISTTH
Nearest Stachelhaus code(s):: Gly DILQFGLIWK (44% 8Å match)
Stachelhaus code match:: 70% (weak)

MZC64_RS40475: X

AMP-binding (33..497): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SAGL-GARMVDGMGQVGGELAKAGLDECLRAVSG
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

MZC64_RS40480: Ile

Search NORINE for peptide: strict or relaxed

AMP-binding (446..836): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: LEDCFDGASYEGVIYFGGEVHSYGPTENSFTSTY
Nearest Stachelhaus code(s):: OH-Orn DGEVVGGVTK (44% 8Å match); Fo-OH-Orn DGEVCGGVTK (38% 8Å match)
Stachelhaus code match:: 60% (weak)

MZC64_RS48980: Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (462..868): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LATHFDFSVWEGSQTFGGEVNMYGITETTVHVTQ
Nearest Stachelhaus code(s):: Thr DFWSVGMVHK (94% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
CRP	Cyclic AMP receptor protein
CelR	Cellobiose uptake repressor
DmdR1	Iron(II)-dependent repressor
OsdR	Development and stress management regulator
RpoN	Regulator of nitrogen assimilation and virulence

NZ_JALMDK010000016.1 - Region 2 - indole,terpene

Download region GenBank file

Download region SVG

Location: 116,595 - 144,886 nt. (total: 28,292 nt) Show pHMM detection rules used

indole: (indsynth or dmat or indole_PTase)
terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

MZC64_RS40785: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

MZC64_RS40830: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

MZC64_RS40840: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

MZC64_RS40860: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

MZC64_RS48990: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: methyltransferase

MZC64_RS40760: methyltransferase

smcogs: SMCOG1089: methyltransferase

Hydrolases: hydrolase

MZC64_RS40805: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Lyases: cyclase

MZC64_RS40835: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Ligases: ligase

MZC64_RS40825: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000683.3	0.91	terpene	avermitilol	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001746.3	0.91	terpene	pristinol	Streptomyces pristinaespiralis ATCC 25486
BGC0002135.2	0.53	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001261.3	0.53	NRPS	AM-toxin	Alternaria alternata
BGC0000307.4	0.53	NRPS	AbT1	Aureobasidium pullulans
BGC0001166.5	0.52	NRPS	HC-toxin	Alternaria jesenskae
BGC0001250.4	0.52	NRPS	epichloenin A	Epichloe festucae
BGC0001251.4	0.52	NRPS	epichloenin A	Epichloe festucae
BGC0001240.4	0.52	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001249.5	0.52	NRPS	dimethylcoprogen	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000683.3	0.76	terpene	avermitilol	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001746.3	0.76	terpene	pristinol	Streptomyces pristinaespiralis ATCC 25486
BGC0002135.2	0.60	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001261.3	0.60	NRPS	AM-toxin	Alternaria alternata
BGC0000307.4	0.60	NRPS	AbT1	Aureobasidium pullulans
BGC0001166.5	0.60	NRPS	HC-toxin	Alternaria jesenskae
BGC0001250.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0001251.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0001240.4	0.60	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001249.5	0.59	NRPS	dimethylcoprogen	Alternaria alternata

Similar known clusters from MIBiG 4.0

Similar subclusters

Detailed terpene predictions

Product predictions:

No product predictions

Genes with terpene domains:

MZC64_RS40835: T1TS

Domain 1 (24 - 325): Type 1 terpene synthase

Subtype: unknown

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.41 out of 20.97

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 24.07 out of 30.11

Confidence: medium

Additional weak hits (8):

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.16 out of 31.73

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 19.59 out of 33.73

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.44 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.76 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.28 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.04 out of 26.06

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.19 out of 29.65

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.20 out of 30.11

Confidence: weak

Terpene

TFBS Finder

Glossary

No acronyms to display

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A
CelR	Cellobiose uptake repressor

NZ_JALMDK010000017.1 - Region 1 - lanthipeptide-class-ii

Download region GenBank file

Download region SVG

Location: 30,251 - 72,720 nt. (total: 42,470 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: sulfotransferase

MZC64_RS41405: Sulfotransferase domain

extras: Sulfotransfer_3: Sulfotransferase domain

Hydrolases: esterase

MZC64_RS41370: thioesterase superfamily protein

smcogs: SMCOG1144: thioesterase superfamily protein

Lyases: cyclase

MZC64_RS41340: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

MZC64_RS41395: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000554.4	0.58	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000527.5	0.55	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0001210.5	0.55	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000516.4	0.54	ribosomal	geobacillin II	Geobacillus thermodenitrificans
BGC0001555.4	0.52	ribosomal	colicin V	Escherichia coli chi7122
BGC0002005.2	0.51	ribosomal	RaxX	Xanthomonas oryzae pv. oryzae
BGC0000590.5	0.51	ribosomal	microcin N	Escherichia coli
BGC0000525.5	0.51	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0002698.2	0.50	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0000528.5	0.50	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000554.4	0.58	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000527.5	0.55	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0001210.5	0.55	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000516.4	0.54	ribosomal	geobacillin II	Geobacillus thermodenitrificans
BGC0001555.4	0.52	ribosomal	colicin V	Escherichia coli chi7122
BGC0002005.2	0.51	ribosomal	RaxX	Xanthomonas oryzae pv. oryzae
BGC0000590.5	0.51	ribosomal	microcin N	Escherichia coli
BGC0000525.5	0.51	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0002698.2	0.50	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0000528.5	0.50	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for MZC64_RS41340

MZC64_RS41345

Leader:

MDIVRVWKDAEYRRSLGVVAHPAGEVDLSDATLATVAGG

Core:

AAGTWVCPSKFLCQTTQLTTTTTTAQTAKSVCHCP

Core with dehydrations:

AAGDhbWVCysPDhaKFLCysQDhbDhbQLDhbDhbDhbDhbDhbDhbAQDhbAKDhaVCysHCysP

antiSMASH-DB 4.0 matches for MZC64_RS41345

100.0%

NZ_JALMDK010000017.1:

Lanthipeptides: MZC64_RS41345_lanthipeptide

1

AAGTWVCPSKFLCQTTQLTTTTTTAQTAKSVCHCP

35

AAGTWVCPSKFLCQTTQLTTTTTTAQTAKSVCHCP

1

AAGTWVCPSKFLCQTTQLTTTTTTAQTAKSVCHCP

35

17.1%

NZ_CP016279.1:

Lanthipeptides: AVL59_RS25175_lanthipeptide

4

TWVCPSKF

11

TW CPS F

23

TWACPSSF

30

MIBiG 4.0 matches for MZC64_RS41345

8.6%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

32

CHC

34

CHC

26

CHC

28

Lanthipeptide(s) for MZC64_RS41395

allorf_059127_059285

Leader:

MTPFRRNENFSVMYA

Core:

EIAYKGEPISGSWWVGVGAVAAMCAACINGVWAPYAS

Core with dehydrations:

EIAYKGEPIDhaGDhaWWVGVGAVAAMCysAACysINGVWAPYADha

antiSMASH-DB 4.0 matches for allorf_059127_059285

100.0%

NZ_JALMDK010000017.1:

Lanthipeptides: allorf_059127_059285_lanthipeptide

1

EIAYKGEPISGSWWVGVGAVAAMCAACINGVWAPYAS

37

EIAYKGEPISGSWWVGVGAVAAMCAACINGVWAPYAS

1

EIAYKGEPISGSWWVGVGAVAAMCAACINGVWAPYAS

37

15.6%

NZ_CP009280.1and 1 other:

NZ_CP009280.1 Lanthipeptides: allorf_0384816_0385007_lanthipeptide

Lanthipeptides: allorf_0384816_0385007_lanthipeptide

15

VGVGAVAAMCAACINGVWAPY

35

+G G V CIN + Y

8

IGAGEVTFRLKRCINALLRVY

28

13.5%

NZ_CP022111.1:

Lanthipeptides: allorf_0450476_0450709_lanthipeptide

1

EIAYKGEPISGSW

13

+ +G+P SG W

1

RFSRRGQPRSGGW

13

MIBiG 4.0 matches for allorf_059127_059285

10.8%

BGC0002144.3 (RCCGEPOP_RS37510):

LAP: phazolicin

22

AMCAAC

27

A CA C

1

ATCARC

6

allorf_059288_059443

Leader:

MATQSVTDWTSTSNPAGLGFGELGREDLMEERVAAM

Core:

ASGGYICTLTWECNC

Core with dehydrations:

ADhaGGYICysDhbLDhbWECysNCys

antiSMASH-DB 4.0 matches for allorf_059288_059443

100.0%

NZ_JALMDK010000017.1:

Lanthipeptides: allorf_059288_059443_lanthipeptide

1

ASGGYICTLTWECNC

15

ASGGYICTLTWECNC

1

ASGGYICTLTWECNC

15

60.0%

NZ_LWKZ01000001.1:

Lanthipeptides: A6A06_RS41385_lanthipeptide

2

SGGYICTLTWECNC

15

S GY CT+T EC C

2

SSGYFCTITTECFC

15

45.5%

NZ_CP034669.1:

Lanthipeptides: EJ065_RS32040_lanthipeptide

2

SGGYICTLTWECNC

15

S GY+CTLT EC C

9

SCGYVCTLTTECGC

22

with 86 more sequence matches

Only the first 10 are displayed here.

42.9%

NZ_AUBN01000022.1:

Lanthipeptides: G448_RS16025_lanthipeptide

1

ASGGYICTLTWEC

13

AS G+ CTLT EC

1

ASSGWFCTLTIEC

13

42.9%

NZ_CP061274.1and 1 other:

NZ_JADKRR010000001.1 Lanthipeptides: ITJ40_RS10085_lanthipeptide

Lanthipeptides: H9X71_RS09850_lanthipeptide

1

ASGGYICTLTWEC

13

AS G++CTLT EC

1

ASSGWLCTLTIEC

13

42.9%

NZ_CP086242.1and 1 other:

NZ_CP086245.1 Lanthipeptides: LL037_RS25615_lanthipeptide

Lanthipeptides: LL038_RS25355_lanthipeptide

1

ASGGYICTLTWECN

14

AS G ICT T EC

1

ASSGAICTATTECT

14

40.0%

NZ_CP036455.1and 4 others:

NZ_JAGPXN010000019.1 Lanthipeptides: KBX06_RS22045_lanthipeptideNZ_JAODUN010000003.1 Lanthipeptides: BXY51_RS10560_lanthipeptideNZ_KB895893.1 Lanthipeptides: C569_RS0129325_lanthipeptideNZ_KB900614.1 Lanthipeptides: SALPAC6_RS0114155_lanthipeptide

Lanthipeptides: EKD16_RS25235_lanthipeptide

2

SGGYICTLTWEC

13

S G++CTLT EC

1

SSGWVCTLTIEC

12

38.1%

NC_009480.1and 10 others:

NZ_CP033724.1 Lanthipeptides: EFE39_RS10220_lanthipeptideNZ_CP047051.1 Lanthipeptides: GRD74_RS10365_lanthipeptideNZ_CP047054.1 Lanthipeptides: GRD61_RS10460_lanthipeptideNZ_CP053860.1 Lanthipeptides: DQP62_RS10825_lanthipeptideNZ_CP076352.1 Lanthipeptides: KN218_RS09900_lanthipeptideNZ_CP085137.1 Lanthipeptides: LHJ48_RS10290_lanthipeptideNZ_CP085140.1 Lanthipeptides: LHJ47_RS10305_lanthipeptideNZ_CP095274.1 Lanthipeptides: MU580_RS10460_lanthipeptideNZ_PSTW01000024.1 Lanthipeptides: C5C03_RS09675_lanthipeptideNZ_SISU01000002.1 Lanthipeptides: EOG37_RS09890_lanthipeptide

Lanthipeptides: CMM_RS10575_lanthipeptide

1

ASGGYICTLTWEC

13

+S G++CTLT EC

1

SSSGWLCTLTIEC

13

38.1%

NZ_CP015515.1and 1 other:

NZ_PSUO01000011.1 Lanthipeptides: C5C18_RS08595_lanthipeptide

Lanthipeptides: A6122_RS12855_lanthipeptide

2

SGGYICTLTWECN

14

S G++CTLT EC

2

SSGWMCTLTIECG

14

38.1%

NZ_CP028130.1:

Lanthipeptides: C7V51_RS14690_lanthipeptide

1

ASGGYICTLTWECN

14

S G++CTLT EC

1

GSSGWMCTLTIECG

14

37.0%

NZ_CP071875.1:

Lanthipeptides: J4I02_RS02880_lanthipeptide

2

SGGYICTLTWEC

13

SGG ICTLT EC

7

SGGAICTLTHEC

18

34.6%

NC_017912.1and 15 others:

NZ_BORO01000007.1 Lanthipeptides: KI343_RS07745_lanthipeptideNZ_CP007242.1 Lanthipeptides: KHU1_RS17410_lanthipeptideNZ_CP031424.1 Lanthipeptides: DXX91_RS18935_lanthipeptideNZ_CP032855.1 Lanthipeptides: D9C11_RS10440_lanthipeptideNZ_CP043809.1 Lanthipeptides: ETZ92_RS14260_lanthipeptideNZ_CP049904.1 Lanthipeptides: V753_RS18470_lanthipeptideNZ_CP053764.1 Lanthipeptides: HPQ66_RS18325_lanthipeptideNZ_CP061938.1 Lanthipeptides: IEN91_RS19800_lanthipeptideNZ_CP063768.1 Lanthipeptides: IM712_RS05205_lanthipeptideNZ_CP087137.1 Lanthipeptides: LOK79_RS18580_lanthipeptideNZ_CP092383.1 Lanthipeptides: MID02_RS19390_lanthipeptideNZ_JAEACM010000001.1 Lanthipeptides: I5F15_RS04355_lanthipeptideNZ_JAJNPN010000015.1 Lanthipeptides: LQ568_RS13285_lanthipeptideNZ_SDKF01000002.1 Lanthipeptides: EUA41_RS09950_lanthipeptideNZ_VDUT01000007.1 Lanthipeptides: FS412_RS14545_lanthipeptide

Lanthipeptides: MUS_RS20065_lanthipeptide

3

GGYICTLTWECNC

15

GG +CTLT EC C

14

GGGVCTLTSECIC

26

33.3%

NZ_CP114875.1:

Lanthipeptides: O4Z47_RS14375_lanthipeptide

2

SGGYICTLTWECN

14

S G ICTLT EC+

7

SSGDICTLTSECD

19

MIBiG 4.0 matches for allorf_059288_059443

26.7%

BGC0002110.3 (QKV49893.1):

Lanthipeptide: birimositide

2

SGGYICTLTWEC

13

+GG+ CTLT EC

15

NGGW-CTLTKEC

25

25.0%

BGC0000517.4 (BAB04173.1):

Lanthipeptide: haloduracin β or haloduracin α

4

GYICTLTWEC

13

G CTLT EC

14

GAYCTLTVEC

23

12.8%

BGC0001229.4 (SBI_06990):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

7

CTLTWECN

14

CTL+ EC

28

CTLSKECQ

35

allorf_059370_059591

Leader:

MKERSALSVIPRLPHDSAHFSCQAGAAGGAPSAPPAAHNDLAAA

Core:

LCRYRVSSCTPRSECRCNLRWPWQRRAPP

Core with dehydrations:

LCysRYRVDhaDhaCysDhbPRDhaECysRCysNLRWPWQRRAPP

antiSMASH-DB 4.0 matches for allorf_059370_059591

100.0%

NZ_JALMDK010000017.1:

Lanthipeptides: allorf_059370_059591_lanthipeptide

1

LCRYRVSSCTPRSECRCNLRWPWQRRAPP

29

LCRYRVSSCTPRSECRCNLRWPWQRRAPP

1

LCRYRVSSCTPRSECRCNLRWPWQRRAPP

29

20.7%

NZ_CP051884.1and 2 others:

NZ_CP051884.1 Lanthipeptides: allorf_3265908_3266039_lanthipeptideNZ_CP051884.1 Lanthipeptides: allorf_3265908_3266039_lanthipeptide

Lanthipeptides: allorf_3265908_3266039_lanthipeptide

22

PWQRRAPP

29

PW RR PP

20

PWGRRGPP

27

13.7%

NZ_CP020040.1:

Lanthipeptides: allorf_284366_284596_lanthipeptide

20

RWPWQRRAPP

29

RW Q RAPP

32

RWQIQSRAPP

41

MIBiG 4.0 matches for allorf_059370_059591

13.8%

BGC0000517.4 (BAB04173.1):

Lanthipeptide: haloduracin β or haloduracin α

5

RVSSCTPRSEC

15

+ + CT EC

13

KGAYCTLTVEC

23

13.3%

BGC0002110.3 (QKV49893.1):

Lanthipeptide: birimositide

9

CTPRSECR

16

CT EC+

19

CTLTKECQ

26

12.8%

BGC0001229.4 (SBI_06990):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

9

CTPRSECRCN

18

CT EC+ N

28

CTLSKECQPN

37

allorf_059489_059593

Leader:

MGQTALRPRHPPDKRNEQNHAG

Core:

DAVSRSARCVLS

Core with dehydrations:

DAVDhaRDhaARCysVLDha

antiSMASH-DB 4.0 matches for allorf_059489_059593

100.0%

NZ_JALMDK010000017.1:

Lanthipeptides: allorf_059489_059593_lanthipeptide

1

DAVSRSARCVLS

12

DAVSRSARCVLS

1

DAVSRSARCVLS

12

allorf_071404_071634

Leader:

MALCACLLRREGAGFPLECGKSCLVVKGVYQWSRLSWLRSPSNSAPVIRSSDFMTSLGG

Core:

ARLCATQAPCFEFCNVC

Core with dehydrations:

ARLCysADhbQAPCysFEFCysNVCys

antiSMASH-DB 4.0 matches for allorf_071404_071634

100.0%

NZ_JALMDK010000017.1:

Lanthipeptides: allorf_071404_071634_lanthipeptide

1

ARLCATQAPCFEFCNVC

17

ARLCATQAPCFEFCNVC

1

ARLCATQAPCFEFCNVC

17

16.7%

NZ_CP050504.1:

Lanthipeptides: allorf_0161193_0161369_lanthipeptide

1

ARLCATQAPCFEFCNV

16

AR C T P +E C

8

ARPCGTACPGYEHCRA

23

14.3%

NZ_CP097263.1:

Lanthipeptides: M3Q35_RS22105_lanthipeptide

1

ARLCATQAPCFE

12

AR+C T A C +

27

ARVCLTDASCHK

38

with 1 more sequence matches

10.3%

NZ_CP101913.1:

Lanthipeptides: allorf_1841460_1841675_lanthipeptide

3

LCATQAPCFEFCNVC

17

+ +T A F F N+C

10

IASTTATTFPFINLC

24

allorf_071480_071626

Leader:

MCLPFAPRGRRFSVGM

Core:

REKLPRCEGGVSMVKIELVEESIELRACNSIF

Core with dehydrations:

REKLPRCysEGGVDhaMVKIELVEEDhaIELRACysNDhaIF

antiSMASH-DB 4.0 matches for allorf_071480_071626

100.0%

NZ_JALMDK010000017.1:

Lanthipeptides: allorf_071480_071626_lanthipeptide

1

REKLPRCEGGVSMVKIELVEESIELRACNSIF

32

REKLPRCEGGVSMVKIELVEESIELRACNSIF

1

REKLPRCEGGVSMVKIELVEESIELRACNSIF

32

25.0%

NZ_AQRJ01000038.1and 1 other:

NZ_AQRJ01000038.1 Lanthipeptides: YW7_RS06850_lanthipeptide

Lanthipeptides: YW7_RS06850_lanthipeptide

5

PRCEGGVSMVKIELVEES

22

P C+GG S VE+S

13

PTCQGGASACN-SFVEDS

29

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.34 out of 20.97

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 19.02 out of 25.31

Confidence: medium

Additional weak hits (11):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.45 out of 30.22

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.64 out of 21.84

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.78 out of 21.55

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.10 out of 29.38

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.69 out of 30.97

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.44 out of 26.85

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 19.40 out of 26.12

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.45 out of 31.73

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.25 out of 21.55

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.64 out of 26.85

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.48 out of 33.14

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

MZC64_RS41345 - Class II

Cleavage pHMM score: 0.60

RODEO score: 0

Monoisotopic mass: 3468.7 Da

Molecular weight: 3471.0 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 3489.0 Da N = 1; 3507.0 Da N = 2; 3525.0 Da N = 3; 3543.0 Da N = 4; 3561.1 Da N = 5; 3579.1 Da N = 6; 3597.1 Da N = 7; 3615.1 Da N = 8

allorf_059127_059285 - Class II

Cleavage pHMM score: -5.30

RODEO score: 0

Monoisotopic mass: 3683.8 Da

Molecular weight: 3686.3 Da

Number of bridges: 1

Additional modifications: AviCys

Alternative weights: (assuming N unmodified Ser/Thr residues); 3704.3 Da N = 1

allorf_059288_059443 - Class II

Cleavage pHMM score: -6.00

RODEO score: 0

Monoisotopic mass: 1519.6 Da

Molecular weight: 1520.8 Da

Number of bridges: 2

Additional modifications: AviCys

allorf_059370_059591 - Class II

Cleavage pHMM score: -5.50

RODEO score: 0

Monoisotopic mass: 3457.7 Da

Molecular weight: 3460.1 Da

Number of bridges: 3

Additional modifications: AviCys

allorf_059489_059593 - Class II

Cleavage pHMM score: -6.60

RODEO score: 0

Monoisotopic mass: 1162.6 Da

Molecular weight: 1163.4 Da

Number of bridges: 0

Additional modifications: AviCys

Alternative weights: (assuming N unmodified Ser/Thr residues); 1181.4 Da N = 1; 1199.4 Da N = 2

allorf_071404_071634 - Class II

Cleavage pHMM score: -5.00

RODEO score: 0

Monoisotopic mass: 1810.8 Da

Molecular weight: 1812.2 Da

Number of bridges: 0

Additional modifications: AviCys

allorf_071480_071626 - Class II

Cleavage pHMM score: -6.10

RODEO score: 0

Monoisotopic mass: 3533.9 Da

Molecular weight: 3536.2 Da

Number of bridges: 1

Additional modifications: AviCys

Alternative weights: (assuming N unmodified Ser/Thr residues); 3554.2 Da N = 1

TFBS definitions

Regulator	Description
CatR	H2O2-responsive repressor
CcpA	Catabolite control protein A

NZ_JALMDK010000017.1 - Region 2 - arylpolyene,lanthipeptide-class-iii,terpene

Download region GenBank file

Download region SVG

Location: 94,554 - 171,683 nt. (total: 77,130 nt) Show pHMM detection rules used

arylpolyene: (APE_KS1 or APE_KS2)
lanthipeptide-class-iii: (micKC and not PKS_KS)
terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

Terpene

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

MZC64_RS41565: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

MZC64_RS41655: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

MZC64_RS41690: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

MZC64_RS41795: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

MZC64_RS41810: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Transferases: aminotransferase

MZC64_RS41590: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

MZC64_RS41670: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Hydrolases: hydrolase protease

MZC64_RS41660: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

MZC64_RS41830: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: cyclase

MZC64_RS41770: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

MZC64_RS41785: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

MZC64_RS41790: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Ligases: ligase synthase synthetase

MZC64_RS41665: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

MZC64_RS41805: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

MZC64_RS49000: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.54	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0000673.3	0.41	terpene	pimara-8(14),15-diene	Aspergillus nidulans FGSC A4
BGC0002309.2	0.36	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000519.4	0.35	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000551.5	0.34	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000501.3	0.33	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0002007.2	0.33	terpene	atolypene A, atolypene B	Amycolatopsis tolypomycina
BGC0001605.3	0.32	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0000677.5	0.31	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001647.3	0.30	PKS	lagunapyrone A, lagunapyrone B, lagunapyrone C	Streptomyces sp. MP131-18

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.81	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0000118.5	0.58	PKS	pactamycin	Streptomyces pactum
BGC0002162.3	0.57	terpene	sesterfisherol, sesterfisheric acid, compound 6, compound 7a, compound 5, compound 8	Aspergillus fischeri NRRL 181
BGC0000657.5	0.55	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000513.3	0.54	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000519.4	0.53	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000551.5	0.53	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000673.3	0.53	terpene	pimara-8(14),15-diene	Aspergillus nidulans FGSC A4
BGC0002309.2	0.53	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000496.5	0.53	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for MZC64_RS41735

allorf_138249_138365

Leader:

MVILDLQSLEPDDVQQGCGAS

Core:

NFSIGCSFSVLCTGKGM

Core with dehydrations:

NFDhaIGCysDhaFDhaVLCysDhbGKGM

antiSMASH-DB 4.0 matches for allorf_138249_138365

100.0%

NZ_JALMDK010000017.1:

Lanthipeptides: allorf_138249_138365_lanthipeptide

1

NFSIGCSFSVLCTGKGM

17

NFSIGCSFSVLCTGKGM

1

NFSIGCSFSVLCTGKGM

17

13.5%

NZ_JAENHX010000011.1:

Lanthipeptides: JIN86_RS20375_lanthipeptide

1

NFSIGCSFSVLCTG

14

N+ + C++S C G

20

NYGMACTWSAECNG

33

12.5%

NZ_KZ859514.1and 1 other:

NZ_KZ859514.1 Lanthipeptides: DPQ64_RS19150_lanthipeptide

Lanthipeptides: DPQ64_RS19155_lanthipeptide

2

FSIGCSFSVLCTGK

15

+S+ C+ S LC+G

27

WSLSCTDSCLCSGD

40

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene		germacradienol	C15	C1-C10	OH

Genes with terpene domains:

MZC64_RS41770: PT_FPPS_like + T1TS

Domain 1 (49 - 335): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Domain 2 (326 - 682): Type 1 terpene synthase

Subtype: unknown

MZC64_RS41785: T1TS

Domain 1 (3 - 324): Type 1 terpene synthase

Subtype: Geosmin synthase, N terminal domain

Reactions:: FPP → germacradienol

MZC64_RS41790: T1TS

Domain 1 (1 - 311): Type 1 terpene synthase

Subtype: unknown

MZC64_RS41805: PT_FPPS_like

Domain 1 (55 - 339): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.00 out of 20.47

Confidence: medium

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.55 out of 18.46

Confidence: strong

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 22.35 out of 29.72

Confidence: medium

Additional weak hits (21):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.46 out of 27.78

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.62 out of 31.73

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.61 out of 32.74

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.18 out of 29.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.36 out of 29.38

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.24 out of 26.06

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.42 out of 26.85

Confidence: weak

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 23.69 out of 29.51

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.46 out of 25.88

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.46 out of 26.06

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.46 out of 25.75

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.97 out of 30.22

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.43 out of 27.78

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.87 out of 31.73

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.94 out of 32.93

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.74 out of 24.78

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.34 out of 35.63

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.34 out of 29.65

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.63 out of 26.85

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.05 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.86 out of 28.19

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptides

NRPS/PKS substrates

Terpene

TFBS Finder

Core peptide predictions

allorf_138249_138365 - Class III

Cleavage pHMM score: 2.90

RODEO score: 0

Monoisotopic mass: 1677.8 Da

Molecular weight: 1679.0 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 1697.0 Da N = 1; 1715.0 Da N = 2

NRPS/PKS substrate predictions

MZC64_RS41665: X

AMP-binding (11..378): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: EAYHFGLCAL-APGLTAGMGQVYGTTETGPICVR
Nearest Stachelhaus code(s):: Leu DALVLAVSIK (35% 8Å match)
Stachelhaus code match:: 60% (weak)

Link to PARAS substrate predictor

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate

TFBS definitions

Regulator	Description
NtrC	Nitrogen regulatory protein C
RpoN	Regulator of nitrogen assimilation and virulence
VqsM	Regulator of quorum-sensing signalling systems

NZ_JALMDK010000017.1 - Region 3 - NRPS,T1PKS

Download region GenBank file

Download region SVG

Location: 180,512 - 230,412 nt. (total: 49,901 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase oxygenase

MZC64_RS42010: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

MZC64_RS42030: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: acyltransferase aminotransferase glycosyltransferase transferase

MZC64_RS41955: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

MZC64_RS41965: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

MZC64_RS41980: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

MZC64_RS41995: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

MZC64_RS42000: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

Hydrolases: hydrolase

MZC64_RS41920: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Ligases: ligase synthase

MZC64_RS41990: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

MZC64_RS42020: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

MZC64_RS42025: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001244.3	0.74	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.73	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.73	PKS	asperlactone	Aspergillus ochraceus
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001261.3	0.65	NRPS	AM-toxin	Alternaria alternata
BGC0001166.5	0.63	NRPS	HC-toxin	Alternaria jesenskae
BGC0001833.4	0.62	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.62	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002192.3	0.61	PKS	FR901512	fungal sp. No.14919
BGC0001132.4	0.61	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001244.3	0.74	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.74	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.74	PKS	asperlactone	Aspergillus ochraceus
BGC0002135.2	0.67	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001261.3	0.66	NRPS	AM-toxin	Alternaria alternata
BGC0000041.3	0.65	PKS	oronofacic acid	Pseudomonas syringae
BGC0001166.5	0.65	NRPS	HC-toxin	Alternaria jesenskae
BGC0001833.4	0.65	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.64	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002192.3	0.64	PKS	FR901512	fungal sp. No.14919

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 19.52 out of 19.52

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.38 out of 19.26

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.56 out of 23.79

Confidence: medium

Additional weak hits (5):

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.03 out of 19.26

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.87 out of 21.55

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.83 out of 21.55

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.61 out of 23.43

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 21.72 out of 35.63

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 6, location 180511 - 230412:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly) + (mal - ohmal) + (pk - mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 7, location 180511 - 223613:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly) + (mal - ohmal) + (pk - mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 8, location 191839 - 230412:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly) + (mal - ohmal) + (pk - mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MZC64_RS41990: Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (461..843): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: VHFTFDISELELQASSGGEWNFYGPTETTIWSTT
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (79% 8Å match)
Stachelhaus code match:: 80% (moderate)

MZC64_RS42020: mal - mal

PKS_AT (530..813): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 170.9
inactive: 88.7
Methoxymalonyl-CoA: 49.7
Methylmalonyl-CoA: 32.1
Isobutyryl-CoA: 17.1
fatty_acid: 14.8
Propionyl-CoA: 13.7
Benzoyl-CoA: 10.9
Acetyl-CoA: 10.9
Ethylmalonyl-CoA: 9.5
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (1491..1765): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 170.7
inactive: 87.3
Methoxymalonyl-CoA: 50.1
Methylmalonyl-CoA: 31.4
Isobutyryl-CoA: 21.4
fatty_acid: 17.6
Propionyl-CoA: 17.2
Acetyl-CoA: 11.6
Benzoyl-CoA: 10.4
trans-1,2-CPDA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

MZC64_RS42025: pk - mal

PKS_AT (564..860): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 202.8
Methylmalonyl-CoA: 202.5
Methoxymalonyl-CoA: 160.4
2-Methylbutyryl-CoA: 132.3
trans-1,2-CPDA: 123.0
Malonyl-CoA: 121.5
Propionyl-CoA: 120.8
CHC-CoA: 87.9
Isobutyryl-CoA: 70.4
Benzoyl-CoA: 53.2
inactive: 41.7
Acetyl-CoA: 40.5
3-Methylbutyryl-CoA: 28.1
fatty_acid: 27.2

PKS_AT (2270..2544): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 167.7
inactive: 85.2
Methoxymalonyl-CoA: 52.0
Methylmalonyl-CoA: 31.7
fatty_acid: 19.2
Isobutyryl-CoA: 17.7
Propionyl-CoA: 17.1
Benzoyl-CoA: 10.2
trans-1,2-CPDA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
CRP	Cyclic AMP receptor protein
IolR	Regulator of the inositol catabolism

NZ_JALMDK010000018.1 - Region 1 - NRPS,NRPS-like,T1PKS

Download region GenBank file

Download region SVG

Location: 137,264 - 223,194 nt. (total: 85,931 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

MZC64_RS42635: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

MZC64_RS42645: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

MZC64_RS42760: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

MZC64_RS42785: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

MZC64_RS42825: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

MZC64_RS42835: lysine/ornithine N-monooxygenase

smcogs: SMCOG1080: lysine/ornithine N-monooxygenase

Transferases: aminotransferase glycosyltransferase methyltransferase

MZC64_RS42665: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

MZC64_RS42685: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

MZC64_RS42720: methyltransferase

smcogs: SMCOG1248: methyltransferase

MZC64_RS42735: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

MZC64_RS42830: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

MZC64_RS42855: glycosyltransferase

smcogs: SMCOG1102: glycosyltransferase

Hydrolases: esterase hydrolase

MZC64_RS42650: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

MZC64_RS42790: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

MZC64_RS42755: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

MZC64_RS42705: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

MZC64_RS42780: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

MZC64_RS42805: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

MZC64_RS42810: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001132.4	1.14	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	1.13	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	1.12	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	1.08	NRPS	AM-toxin	Alternaria alternata
BGC0001758.4	1.07	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	1.07	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	1.06	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001825.4	1.06	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002075.3	1.05	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001641.4	1.04	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.77	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002286.3	0.77	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.77	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.77	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.77	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.76	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002075.3	0.76	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001758.4	0.75	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001825.4	0.75	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001261.3	0.75	NRPS	AM-toxin	Alternaria alternata

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.33 out of 27.78

Confidence: medium

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 16.04 out of 18.57

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 20.63 out of 28.19

Confidence: medium

Additional weak hits (23):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.27 out of 30.22

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.81 out of 21.84

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.48 out of 27.78

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.38 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.33 out of 29.38

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 20.39 out of 36.54

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 24.45 out of 36.54

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.84 out of 21.84

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 19.58 out of 31.73

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.42 out of 26.85

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.50 out of 27.78

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.49 out of 30.97

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.26 out of 27.78

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.32 out of 28.14

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.77 out of 21.55

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.04 out of 30.25

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.54 out of 28.14

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.70 out of 20.97

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.09 out of 29.38

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.03 out of 24.78

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.79 out of 29.25

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 22.52 out of 29.65

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.37 out of 26.06

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 137263 - 223194:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Thr) + (fatty_acid) + (D-X - X - X - Asn - X - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 137263 - 182969:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Thr) + (fatty_acid)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 156661 - 199028:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Thr) + (fatty_acid) + (D-X - X - X - Asn - X - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 164621 - 223194:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Thr) + (fatty_acid) + (D-X - X - X - Asn - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MZC64_RS42705: mal

PKS_AT (618..844): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 72.5
Methoxymalonyl-CoA: 53.3
Methylmalonyl-CoA: 45.6
inactive: 34.9
Propionyl-CoA: 31.1
Isobutyryl-CoA: 25.5
fatty_acid: 19.5
Ethylmalonyl-CoA: 18.8
trans-1,2-CPDA: 7.6
2-Methylbutyryl-CoA: 2.5
3-Methylbutyryl-CoA: 2.4
CHC-CoA: 2.3
Acetyl-CoA: 0.6
Benzoyl-CoA: 0.0

MZC64_RS42780: Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (229..615): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LATHFDFSVWEGSQIFGGEVNMFGITETTVHVTH
Nearest Stachelhaus code(s):: Thr DFWSIGMVHK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

MZC64_RS42805: fatty_acid

CAL_domain (42..478): fatty_acid

minowa: fatty_acid

Prediction, score:

fatty_acid: 66.0
AHBA: 29.3
NH2: 19.3
shikimic_acid: 8.7
Acetyl-CoA: 7.0

MZC64_RS42810: X - X - X - Asn - X - X

Search NORINE for peptide: strict or relaxed

AMP-binding (441..810): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: MWAAFDVSVYEWEFTAGGELNTYGPAETAVNATW
Nearest Stachelhaus code(s):: Dab DVCETGTIEK (38% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (2380..2761): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHLESGDETNAYGPTEAAIDVTR
Nearest Stachelhaus code(s):: Dab DVWHVGAVDK (88% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (3409..3795): X

nrpys: OH-Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWASFDLTVTGGKAVLGGELNEYGPTETTVGCTV
Nearest Stachelhaus code(s):: OH-Asn DLTKVGEVGK (91% 8Å match); Asn DLTKVGEVGK (85% 8Å match); Trp DLTKVGEVGK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4429..4800): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YEASFDMPVTTTKVIIGGETNEYGPTEATVGCVV
Nearest Stachelhaus code(s):: Asn DMTKVGEVGK (91% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (5440..5828): X

nrpys: Ile, Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LDESFDAATYEVFLWTGGEVHVYGPTETTTFATF
Nearest Stachelhaus code(s):: Ile or Val DAYFWGVTFK (91% 8Å match); Val DAYFWGVTFK (88% 8Å match); Leu DAYFWGVTFK (88% 8Å match); Ile DAYFWGVTFK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (6482..6876): X

nrpys: Glu, Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HWLTFTVSVWEWHFFVGGEYYKYGSTEVTCDATF
Nearest Stachelhaus code(s):: Ser DVWHFSLVDK (65% 8Å match)
Stachelhaus code match:: 60% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
ToxT	Regulator of MSHA biosynthesis
ZuR variant 1	Zinc-responsive repressor

NZ_JALMDK010000019.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 23,971 - 87,000 nt. (total: 63,030 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

MZC64_RS42985: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

MZC64_RS43035: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

MZC64_RS43045: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

MZC64_RS43055: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

MZC64_RS43060: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

MZC64_RS43165: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: aminotransferase methyltransferase

MZC64_RS43070: methyltransferase

smcogs: SMCOG1089: methyltransferase

MZC64_RS43150: aminotransferase

smcogs: SMCOG1019: aminotransferase

Hydrolases: hydrolase

MZC64_RS43065: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Ligases: ligase

MZC64_RS42975: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

MZC64_RS43050: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

MZC64_RS43090: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Translocases: permease

MZC64_RS43140: phenylalanine-specific permease

smcogs: SMCOG1038: phenylalanine-specific permease

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.64	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.64	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.64	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.63	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002075.3	0.63	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001135.5	0.63	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000307.4	0.63	NRPS	AbT1	Aureobasidium pullulans
BGC0002135.2	0.63	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.64	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.64	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.64	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.63	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002075.3	0.63	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001135.5	0.63	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000307.4	0.63	NRPS	AbT1	Aureobasidium pullulans
BGC0002135.2	0.63	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 30.03 out of 36.53

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 21.91 out of 28.19

Confidence: medium

Additional weak hits (8):

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 22.23 out of 33.73

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 18.16 out of 30.97

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.46 out of 30.25

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 23.33 out of 36.53

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.50 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 22.43 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.41 out of 29.38

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.60 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 23970 - 87000:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-Cys) + (Ser - Cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MZC64_RS42975: X

AMP-binding (17..385): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FGVGADVVLEEPELLVRGEHHVYGMAETTVTNTV
Nearest Stachelhaus code(s):: Ahp DVENAGVVTK (44% 8Å match)
Stachelhaus code match:: 70% (weak)

MZC64_RS43050: Ser - Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (535..904): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: TDLSFDLSVYDGNCLLGGDAGLGGTTETAIHSTV
Nearest Stachelhaus code(s):: Ser DLYNLGLIHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1541..1929): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: TDISFDLSVYDGNTLVSGDVALGGATEASIWSNA
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

MZC64_RS43090: Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (522..914): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: TDIAFDLSVYDGNSLLSGDVSLGGATEAAIWSIH
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
DmdR1	Iron(II)-dependent repressor
ZuR variant 1	Zinc-responsive repressor

NZ_JALMDK010000019.1 - Region 2 - T1PKS

Download region GenBank file

Download region SVG

Location: 153,661 - 202,948 nt. (total: 49,288 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

MZC64_RS43550: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

MZC64_RS43570: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Hydrolases: amidase hydrolase

MZC64_RS43520: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

MZC64_RS43620: amidase

smcogs: SMCOG1105: amidase

Ligases: synthase

MZC64_RS43565: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

MZC64_RS43575: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

MZC64_RS43580: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001275.3	0.74	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.74	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.74	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.74	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.66	PKS	oronofacic acid	Pseudomonas syringae
BGC0001160.5	0.64	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001257.3	0.64	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001164.3	0.64	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002175.3	0.63	PKS	YWA1	Aspergillus oryzae RIB40

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001275.3	0.74	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.74	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.74	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.74	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.66	PKS	oronofacic acid	Pseudomonas syringae
BGC0001160.5	0.64	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001257.3	0.64	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001164.3	0.64	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002175.3	0.63	PKS	YWA1	Aspergillus oryzae RIB40

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 21.87 out of 29.72

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.81 out of 23.79

Confidence: medium

Additional weak hits (3):

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 19.67 out of 30.97

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.33 out of 29.38

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.96 out of 24.78

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 2, location 153660 - 202948:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal - ohmal) + (ccmal) + (pk)
Non-colinear ordering:: MZC64_RS43565 + MZC64_RS43575 + MZC64_RS43580

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MZC64_RS43565: mmal - mal

PKS_AT (528..821): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 202.5
Ethylmalonyl-CoA: 190.5
Methoxymalonyl-CoA: 162.1
2-Methylbutyryl-CoA: 132.9
Propionyl-CoA: 99.1
trans-1,2-CPDA: 91.7
CHC-CoA: 90.2
Malonyl-CoA: 83.7
Isobutyryl-CoA: 63.5
Benzoyl-CoA: 62.6
fatty_acid: 30.6
3-Methylbutyryl-CoA: 25.9
Acetyl-CoA: 24.4
inactive: 12.3

PKS_AT (2155..2428): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 163.5
Methylmalonyl-CoA: 73.6
Methoxymalonyl-CoA: 70.6
inactive: 62.8
Isobutyryl-CoA: 39.5
Ethylmalonyl-CoA: 33.9
Propionyl-CoA: 24.1
Benzoyl-CoA: 21.0
fatty_acid: 13.8
Acetyl-CoA: 13.0
CHC-CoA: 12.5
trans-1,2-CPDA: 7.7
2-Methylbutyryl-CoA: 6.8
3-Methylbutyryl-CoA: 0.0

MZC64_RS43575: mal

PKS_AT (560..848): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 209.2
inactive: 95.4
Methoxymalonyl-CoA: 64.3
Methylmalonyl-CoA: 61.1
Propionyl-CoA: 31.0
Ethylmalonyl-CoA: 26.6
CHC-CoA: 20.0
Isobutyryl-CoA: 19.9
Acetyl-CoA: 19.7
Benzoyl-CoA: 16.4
2-Methylbutyryl-CoA: 14.7
trans-1,2-CPDA: 13.2
fatty_acid: 8.8
3-Methylbutyryl-CoA: 0.0

MZC64_RS43580: pk

PKS_AT (564..861): pk

ATSignature: Methoxymalonyl-CoA

Top 3 matches:: Methoxymalonyl-CoA: 79.2%; Methylmalonyl-CoA: 75.0%; Ethylmalonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 152.4
Ethylmalonyl-CoA: 134.1
Methoxymalonyl-CoA: 126.8
Malonyl-CoA: 85.8
CHC-CoA: 81.5
Isobutyryl-CoA: 78.1
trans-1,2-CPDA: 75.1
2-Methylbutyryl-CoA: 72.4
Propionyl-CoA: 70.2
Benzoyl-CoA: 66.5
fatty_acid: 27.8
Acetyl-CoA: 25.5
inactive: 22.6
3-Methylbutyryl-CoA: 17.4

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
NtrC	Nitrogen regulatory protein C

NZ_JALMDK010000001.1 - Region 1 - butyrolactone

Download region GenBank file

Download region SVG

Location: 419,356 - 430,255 nt. (total: 10,900 nt) Show pHMM detection rules used

butyrolactone: AfsA

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

MZC64_RS02035: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000849.4	0.77	other (butyrolactone)	SCB1, SCB2, SCB3	Streptomyces coelicolor A3(2)
BGC0000848.5	0.76	other (other)	A-factor	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000850.5	0.68	other (other)	γ-butyrolactone	Streptomyces acidiscabies
BGC0001135.5	0.56	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002277.2	0.56	NRPS	atromentin	Suillus grevillei
BGC0001833.4	0.55	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286.3	0.55	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001160.5	0.52	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.51	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002578.2	0.49	other (other)	gaburedin A, gaburedin B, gaburedin C, gaburedin D, gaburedin E, gaburedin F	Streptomyces venezuelae ATCC 10712

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000849.4	0.77	other (butyrolactone)	SCB1, SCB2, SCB3	Streptomyces coelicolor A3(2)
BGC0000848.5	0.76	other (other)	A-factor	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000850.5	0.68	other (other)	γ-butyrolactone	Streptomyces acidiscabies
BGC0001135.5	0.56	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002277.2	0.56	NRPS	atromentin	Suillus grevillei
BGC0001833.4	0.55	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286.3	0.55	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001160.5	0.52	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.51	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002578.2	0.49	other (other)	gaburedin A, gaburedin B, gaburedin C, gaburedin D, gaburedin E, gaburedin F	Streptomyces venezuelae ATCC 10712

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (3):

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.08 out of 28.19

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.81 out of 20.01

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.17 out of 27.18

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description

NZ_JALMDK010000001.1 - Region 2 - HR-T2PKS

Download region GenBank file

Download region SVG

Location: 677,811 - 719,969 nt. (total: 42,159 nt) Show pHMM detection rules used

HR-T2PKS: (hr-t2pks-ksa and (t2fas or APE_KS2 or ketoacyl-synt))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase protease

MZC64_RS03395: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

MZC64_RS03555: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Lyases: cyclase

MZC64_RS03435: diguanylate cyclase

smcogs: SMCOG1025: diguanylate cyclase

Ligases: synthase

MZC64_RS03485: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000272.3	0.45	PKS	spore pigment	Streptomyces collinus
BGC0002510.2	0.44	PKS	youssoufene A1, youssoufene B1, youssoufene B2, youssoufene B3, youssoufene B4	Streptomyces sp.
BGC0001343.3	0.44	PKS	aurachin A, aurachin D	Stigmatella aurantiaca Sg a15
BGC0001366.5	0.44	PKS	BE-24566B, zunyimycin A	Streptomyces sp. FJS31-2
BGC0000231.5	0.44	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0002629.2	0.43	ribosomal	limnothamide	Limnothrix sp. CACIAM 69d
BGC0000234.5	0.43	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0002591.2	0.42	PKS, terpene	aurachin C, aurachin D, aurachin SS	Streptomyces sp.
BGC0001075.5	0.42	PKS, terpene	aurachin RE	Rhodococcus erythropolis
BGC0001513.3	0.42	PKS	anthrabenzoxocinone	Actinobacteria bacterium

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000272.3	0.45	PKS	spore pigment	Streptomyces collinus
BGC0002510.2	0.44	PKS	youssoufene A1, youssoufene B1, youssoufene B2, youssoufene B3, youssoufene B4	Streptomyces sp.
BGC0001343.3	0.44	PKS	aurachin A, aurachin D	Stigmatella aurantiaca Sg a15
BGC0001366.5	0.44	PKS	BE-24566B, zunyimycin A	Streptomyces sp. FJS31-2
BGC0000231.5	0.44	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0002629.2	0.43	ribosomal	limnothamide	Limnothrix sp. CACIAM 69d
BGC0000234.5	0.43	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0002591.2	0.42	PKS, terpene	aurachin C, aurachin D, aurachin SS	Streptomyces sp.
BGC0001075.5	0.42	PKS, terpene	aurachin RE	Rhodococcus erythropolis
BGC0001513.3	0.42	PKS	anthrabenzoxocinone	Actinobacteria bacterium

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (4):

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.99 out of 29.25

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 19.13 out of 30.97

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.05 out of 30.25

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 20.30 out of 27.04

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

TFBS Finder

TFBS definitions

Regulator	Description

NZ_JALMDK010000001.1 - Region 3 - redox-cofactor

Download region GenBank file

Download region SVG

Location: 869,302 - 891,410 nt. (total: 22,109 nt) Show pHMM detection rules used

redox-cofactor: (cds(PF04055 and (TIGR04085 or SPASM)) and (TIGR02111 or TIGR03966) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acetyltransferase

MZC64_RS04360: acetyl-CoA acetyltransferase

smcogs: SMCOG1124: acetyl-CoA acetyltransferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.43	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002316.2	0.41	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0002346.3	0.41	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0001496.3	0.38	ribosomal	actinokineosin	Actinokineospora spheciospongiae
BGC0000874.5	0.36	other (other)	blasticidin S	Streptomyces griseochromogenes
BGC0002015.4	0.36	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0002307.3	0.36	ribosomal	humidimycin	Streptomyces humidus
BGC0000559.4	0.36	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0001920.4	0.36	ribosomal	siamycin I	Streptomyces sp.
BGC0001674.5	0.35	ribosomal	MS-271	Streptomyces mirabilis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.43	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002316.2	0.41	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0002346.3	0.41	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0001496.3	0.38	ribosomal	actinokineosin	Actinokineospora spheciospongiae
BGC0000874.5	0.36	other (other)	blasticidin S	Streptomyces griseochromogenes
BGC0002015.4	0.36	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0002307.3	0.36	ribosomal	humidimycin	Streptomyces humidus
BGC0000559.4	0.36	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0001920.4	0.36	ribosomal	siamycin I	Streptomyces sp.
BGC0001674.5	0.35	ribosomal	MS-271	Streptomyces mirabilis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.28 out of 19.52

Confidence: medium

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 21.67 out of 27.04

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.41 out of 23.79

Confidence: medium

Additional weak hits (6):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.70 out of 26.33

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 23.79 out of 30.22

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.49 out of 27.78

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.66 out of 20.01

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.37 out of 20.97

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 19.59 out of 33.73

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: PqqD_RRE; Location: 1 - 89; Score: 79.1

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
DasR	N-acetylglucosamine dependent repressor
IolR	Regulator of the inositol catabolism

NZ_JALMDK010000001.1 - Region 4 - T1PKS

Download region GenBank file

Download region SVG

Location: 1,048,811 - 1,095,332 nt. (total: 46,522 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

MZC64_RS05185: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: acetyltransferase

MZC64_RS05175: acetyl-CoA acetyltransferase

smcogs: SMCOG1124: acetyl-CoA acetyltransferase

Hydrolases: esterase hydrolase

MZC64_RS05260: putative esterase

smcogs: SMCOG1104: putative esterase

MZC64_RS05345: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Lyases: dehydratase hydratase

MZC64_RS05170: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

MZC64_RS05190: L-carnitine dehydratase/bile acid-inducible

smcogs: SMCOG1189: L-carnitine dehydratase/bile acid-inducible

MZC64_RS05325: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

MZC64_RS05250: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

MZC64_RS05255: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Translocases: transport

MZC64_RS05270: MMPL domain-containing transport protein

smcogs: SMCOG1035: MMPL domain-containing transport protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001160.5	0.68	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.67	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001254.3	0.64	PKS	ACT-Toxin II	Alternaria alternata
BGC0002191.2	0.64	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001275.3	0.63	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.63	PKS	asperlactone	Aspergillus ochraceus
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001276.3	0.63	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001258.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001160.5	0.68	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.67	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001254.3	0.64	PKS	ACT-Toxin II	Alternaria alternata
BGC0002191.2	0.64	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001275.3	0.63	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.63	PKS	asperlactone	Aspergillus ochraceus
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001276.3	0.63	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001258.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.00 out of 20.47

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.13 out of 19.26

Confidence: medium

Additional weak hits (6):

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.67 out of 28.14

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.67 out of 25.75

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.04 out of 21.84

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.07 out of 30.25

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.67 out of 18.57

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 19.52 out of 27.04

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 4, location 1048810 - 1095332:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MZC64_RS05250: mmal

PKS_AT (623..912): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 79.2%; Ethylmalonyl-CoA: 70.8%; Malonyl-CoA: 70.8%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 131.1
Ethylmalonyl-CoA: 111.6
Malonyl-CoA: 99.6
Methoxymalonyl-CoA: 97.5
trans-1,2-CPDA: 85.2
Propionyl-CoA: 82.2
2-Methylbutyryl-CoA: 81.4
Isobutyryl-CoA: 67.9
CHC-CoA: 58.6
Acetyl-CoA: 55.4
fatty_acid: 53.1
Benzoyl-CoA: 47.4
3-Methylbutyryl-CoA: 40.5
inactive: 39.0

MZC64_RS05255: NH2

CAL_domain (51..494): NH2

minowa: NH2

Prediction, score:

NH2: 158.3
fatty_acid: 19.8
AHBA: 5.4
Acetyl-CoA: 4.3
shikimic_acid: 0.0

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
VqsM	Regulator of quorum-sensing signalling systems

NZ_JALMDK010000001.1 - Region 5 - terpene

Download region GenBank file

Download region SVG

Location: 1,125,863 - 1,152,638 nt. (total: 26,776 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: methyltransferase

MZC64_RS05550: methyltransferase

smcogs: SMCOG1089: methyltransferase

Lyases: cyclase

MZC64_RS05545: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

MZC64_RS05555: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

MZC64_RS05560: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Ligases: synthetase

MZC64_RS05535: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.81	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0000658.3	0.79	terpene	2-methylisoborneol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000657.5	0.78	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000659.3	0.62	terpene	2-methylisoborneol	Saccharopolyspora erythraea NRRL 2338
BGC0002309.2	0.52	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000677.5	0.45	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000640.3	0.44	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0001911.3	0.43	terpene	raimonol	Streptomyces anulatus
BGC0000630.3	0.43	terpene	(2R,3S,3'S)-2-hydroxyastaxanthin	Paracoccus haeundaensis
BGC0000639.3	0.41	terpene	carotenoid	Enterobacteriaceae bacterium DC260

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.81	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0000658.3	0.79	terpene	2-methylisoborneol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000657.5	0.78	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000659.3	0.62	terpene	2-methylisoborneol	Saccharopolyspora erythraea NRRL 2338
BGC0002309.2	0.52	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000677.5	0.45	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000640.3	0.44	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0001911.3	0.43	terpene	raimonol	Streptomyces anulatus
BGC0000630.3	0.43	terpene	(2R,3S,3'S)-2-hydroxyastaxanthin	Paracoccus haeundaensis
BGC0000639.3	0.41	terpene	carotenoid	Enterobacteriaceae bacterium DC260

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
monoterpene	2-methylisoborneol	C11	C1-C6	OH
monoterpene	2-methylenebornane	C11	C1-C6	none
sesquiterpene	germacradienol	C15	C1-C10	OH

Genes with terpene domains:

MZC64_RS05535: PT_FPPS_like

Domain 1 (60 - 347): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

MZC64_RS05545: T1TS

Domain 1 (68 - 383): Type 1 terpene synthase

Subtype: 2-Methylisoborneol synthase type

Reactions:: 2-MeGPP → 2-methylisoborneol; 2-MeGPP → 2-methylenebornane

MZC64_RS05555: T1TS

Domain 1 (11 - 362): Type 1 terpene synthase

Subtype: unknown

MZC64_RS05560: T1TS

Domain 1 (2 - 331): Type 1 terpene synthase

Subtype: Geosmin synthase, N terminal domain

Reactions:: FPP → germacradienol

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (6):

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.46 out of 25.75

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.47 out of 26.06

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.35 out of 30.25

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.60 out of 30.25

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.47 out of 20.97

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.75 out of 27.18

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
2-MeGPP	2-methylgeranyl diphosphate
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate

TFBS definitions

Regulator	Description

NZ_JALMDK010000001.1 - Region 6 - terpene

Download region GenBank file

Download region SVG

Location: 1,164,665 - 1,185,939 nt. (total: 21,275 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001910.4	0.38	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0001911.3	0.37	terpene	raimonol	Streptomyces anulatus
BGC0002303.3	0.37	ribosomal	pristinin A3	Streptomyces pristinaespiralis
BGC0002316.2	0.32	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0002346.3	0.30	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0001374.5	0.29	other (other)	thienodolin	Streptomyces albogriseolus
BGC0002130.3	0.28	ribosomal	cacaoidin	Streptomyces cacaoi CA-170360
BGC0000069.5	0.28	PKS	gephyronic acid	Archangium violaceum Cb vi76
BGC0000503.5	0.27	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002448.2	0.27	NRPS	Q6402A	Streptomyces violaceusniger

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001910.4	0.38	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0001911.3	0.37	terpene	raimonol	Streptomyces anulatus
BGC0002303.3	0.37	ribosomal	pristinin A3	Streptomyces pristinaespiralis
BGC0002316.2	0.32	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0002346.3	0.30	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0001374.5	0.29	other (other)	thienodolin	Streptomyces albogriseolus
BGC0002130.3	0.28	ribosomal	cacaoidin	Streptomyces cacaoi CA-170360
BGC0000069.5	0.28	PKS	gephyronic acid	Archangium violaceum Cb vi76
BGC0000503.5	0.27	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002448.2	0.27	NRPS	Q6402A	Streptomyces violaceusniger

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

No product predictions

Genes with terpene domains:

No domains found

Transcription Factor Binding Sites

Binding sites found:

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.13 out of 26.85

Confidence: medium

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.22 out of 18.46

Confidence: strong

Additional weak hits (10):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.51 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.55 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.03 out of 27.78

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.75 out of 30.25

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 20.05 out of 27.04

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.34 out of 26.06

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.56 out of 28.19

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.63 out of 32.93

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.78 out of 32.93

Confidence: weak

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 17.99 out of 25.31

Confidence: weak

Terpene

TFBS Finder

Glossary

No acronyms to display

TFBS definitions

Regulator	Description
RpoN	Regulator of nitrogen assimilation and virulence
RutR	Regulator of the pyrimidine and purine metabolism

NZ_JALMDK010000020.1 - Region 1 - NRPS-like,T1PKS

Download region GenBank file

Download region SVG

Location: 55,545 - 130,535 nt. (total: 74,991 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

MZC64_RS43945: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

MZC64_RS43975: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Transferases: acylase acyltransferase aminotransferase methyltransferase

MZC64_RS43890: methyltransferase

smcogs: SMCOG1089: methyltransferase

MZC64_RS43930: acyltransferase

smcogs: SMCOG1141: acyltransferase

MZC64_RS43990: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

MZC64_RS44010: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

MZC64_RS44020: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

MZC64_RS44025: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Lyases: decarboxylase

MZC64_RS43935: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

Ligases: ligase synthase

MZC64_RS43955: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

MZC64_RS44015: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

MZC64_RS44040: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001276.3	1.17	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	1.17	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.87	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001284.4	0.85	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000041.3	0.83	PKS	oronofacic acid	Pseudomonas syringae
BGC0002171.2	0.63	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002275.2	0.62	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger CBS 101883
BGC0002191.2	0.60	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001160.5	0.59	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.59	PKS	1-heptadecene	Cyanothece sp. PCC 7822

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.74	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.74	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000041.3	0.69	PKS	oronofacic acid	Pseudomonas syringae
BGC0001160.5	0.66	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.66	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002191.2	0.64	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0000107.3	0.63	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002192.3	0.62	PKS	FR901512	fungal sp. No.14919

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.31 out of 26.85

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

Additional weak hits (12):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.67 out of 26.33

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.81 out of 27.78

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.50 out of 21.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.79 out of 27.78

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.29 out of 18.57

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.14 out of 21.55

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.24 out of 26.06

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.56 out of 28.19

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 19.27 out of 27.04

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 17.22 out of 26.12

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.04 out of 32.74

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 18.13 out of 33.14

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 55544 - 130535:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (AHBA - ccmal) + (AHBA - mal - Me-ccmal) + (pk)
Non-colinear ordering:: MZC64_RS43955 + MZC64_RS44015 + MZC64_RS44010

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MZC64_RS43955: AHBA - mal

CAL_domain (34..376): AHBA

minowa: AHBA

Prediction, score:

AHBA: 108.0
shikimic_acid: 103.6
fatty_acid: 0.0
NH2: 0.0
Acetyl-CoA: 0.0

PKS_AT (954..1211): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 139.0
inactive: 61.6
Methoxymalonyl-CoA: 61.5
Methylmalonyl-CoA: 57.1
Ethylmalonyl-CoA: 34.6
CHC-CoA: 27.1
2-Methylbutyryl-CoA: 25.1
Propionyl-CoA: 20.4
trans-1,2-CPDA: 19.8
Acetyl-CoA: 18.1
Isobutyryl-CoA: 16.5
Benzoyl-CoA: 15.3
fatty_acid: 14.5
3-Methylbutyryl-CoA: 0.0

MZC64_RS44010: pk

PKS_AT (558..845): pk

ATSignature: Methoxymalonyl-CoA

Top 3 matches:: Methoxymalonyl-CoA: 91.7%; Methylmalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 209.2
Methylmalonyl-CoA: 203.9
Methoxymalonyl-CoA: 170.6
2-Methylbutyryl-CoA: 156.8
trans-1,2-CPDA: 121.1
Malonyl-CoA: 115.5
Propionyl-CoA: 112.0
CHC-CoA: 99.6
Isobutyryl-CoA: 82.6
Benzoyl-CoA: 60.9
inactive: 39.8
Acetyl-CoA: 39.4
3-Methylbutyryl-CoA: 27.3
fatty_acid: 26.6

MZC64_RS44015: AHBA - mal - mmal

CAL_domain (36..423): AHBA

minowa: AHBA

Prediction, score:

AHBA: 286.3
shikimic_acid: 231.7
fatty_acid: 0.0
NH2: 0.0
Acetyl-CoA: 0.0

PKS_AT (1293..1573): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 194.3
inactive: 98.0
Methoxymalonyl-CoA: 53.9
Methylmalonyl-CoA: 43.3
Propionyl-CoA: 22.9
Acetyl-CoA: 21.8
CHC-CoA: 13.2
Isobutyryl-CoA: 12.3
Benzoyl-CoA: 10.3
fatty_acid: 9.5
Ethylmalonyl-CoA: 9.5
2-Methylbutyryl-CoA: 8.3
trans-1,2-CPDA: 7.8
3-Methylbutyryl-CoA: 0.0

PKS_AT (2242..2522): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 205.6
Ethylmalonyl-CoA: 199.9
Methoxymalonyl-CoA: 165.0
2-Methylbutyryl-CoA: 143.2
Malonyl-CoA: 113.3
CHC-CoA: 102.8
trans-1,2-CPDA: 101.1
Propionyl-CoA: 100.3
Benzoyl-CoA: 61.2
Isobutyryl-CoA: 60.9
inactive: 41.4
Acetyl-CoA: 37.3
3-Methylbutyryl-CoA: 25.8
fatty_acid: 23.8

MZC64_RS44040: X

AMP-binding (38..435): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LPLPHNATISVSFAYNTGDVSGPGP------SLR
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
RutR	Regulator of the pyrimidine and purine metabolism

NZ_JALMDK010000021.1 - Region 1 - lanthipeptide-class-iii

Download region GenBank file

Download region SVG

Location: 93,534 - 116,092 nt. (total: 22,559 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-iii: (micKC and not PKS_KS)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase synthase

MZC64_RS44560: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

MZC64_RS44565: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001641.4	0.55	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.55	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001164.3	0.52	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000528.5	0.50	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002700.2	0.49	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0000496.5	0.48	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000551.5	0.47	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000519.4	0.46	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000553.3	0.46	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998
BGC0000501.3	0.46	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001641.4	0.55	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.55	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001164.3	0.52	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000528.5	0.50	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002700.2	0.49	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0000496.5	0.48	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000551.5	0.47	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000519.4	0.46	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000553.3	0.46	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998
BGC0000501.3	0.46	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for MZC64_RS44575

MZC64_RS44570

Leader:

MDVLDLQAIEPAEVDGPAE

Core:

FGAGLISTLSLLICW

Core with dehydrations:

FGAGLIDhaDhbLDhaLLICysW

antiSMASH-DB 4.0 matches for MZC64_RS44570

100.0%

NZ_JALMDK010000021.1:

Lanthipeptides: MZC64_RS44570_lanthipeptide

1

FGAGLISTLSLLICW

15

FGAGLISTLSLLICW

1

FGAGLISTLSLLICW

15

41.2%

NZ_JAGFNS010000021.1:

Lanthipeptides: J5X75_RS45620_lanthipeptide

2

GAGLISTLSLLIC

14

A S+LSLL+C

5

AASTDSSLSLLVC

17

40.0%

NZ_FMZZ01000007.1:

Lanthipeptides: BLS56_RS16085_lanthipeptide

7

STLSLLIC

14

STLSLLIC

12

STLSLLIC

19

with 11 more sequence matches

Only the first 10 are displayed here.

34.8%

NZ_CP021121.1and 1 other:

NZ_CP084541.1 Lanthipeptides: LC193_RS06630_lanthipeptide

Lanthipeptides: CAG99_RS21195_lanthipeptide

4

GLISTLSLLIC

14

G +S+LSLLIC

1

GDLSSLSLLIC

11

31.8%

NZ_CP024894.1and 2 others:

NZ_CP091381.1 Lanthipeptides: L3Q65_RS08355_lanthipeptideNZ_SDLS01000004.1 Lanthipeptides: EIZ45_RS09385_lanthipeptide

Lanthipeptides: CU254_RS16295_lanthipeptide

7

STLSLLICW

15

STLSLL+C+

14

STLSLLLCY

22

31.8%

NZ_CP097289.1:

Lanthipeptides: M4V62_RS29260_lanthipeptide

7

STLSLLICW

15

ST SLL+CW

5

STASLLLCW

13

30.8%

NZ_KB913037.1:

Lanthipeptides: A3CE_RS56710_lanthipeptide

7

STLSLLICW

15

STLSLL CW

18

STLSLLTCW

26

30.0%

NZ_BNBL01000010.1and 1 other:

NZ_CP071595.1 Lanthipeptides: J3S04_RS21315_lanthipeptide

Lanthipeptides: IE295_RS24405_lanthipeptide

7

STLSLLICW

15

S SLL+CW

3

SNASLLLCW

11

29.2%

NZ_KB912942.1and 1 other:

NZ_LWSF01000028.1 Lanthipeptides: allorf_078284_078409_lanthipeptide

Lanthipeptides: AMYBE_RS44660_lanthipeptide

7

STLSLLIC

14

STLSLL+C

16

STLSLLLC

23

28.6%

NZ_CP021744.1and 3 others:

NZ_CP097123.1 Lanthipeptides: M1P56_RS05765_lanthipeptideNZ_JACHJF010000016.1 Lanthipeptides: FHS36_RS22695_lanthipeptideNZ_JAJQQQ010000006.1 Lanthipeptides: LUZ28_RS13020_lanthipeptide

Lanthipeptides: SMD11_RS23510_lanthipeptide

7

STLSLLICW

15

S +SLL+CW

4

SNVSLLLCW

12

25.9%

NZ_CP023865.1and 1 other:

NZ_JAGFNS010000004.1 Lanthipeptides: J5X75_RS08295_lanthipeptide

Lanthipeptides: ACTEI_RS22430_lanthipeptide

2

GAGLISTLSLLIC

14

G G+ ST SL++C

4

GCGVESTASLVVC

16

25.0%

NZ_CP064192.1:

Lanthipeptides: IOD16_RS04595_lanthipeptide

1

FGAGLISTLSLLIC

14

G G ST+SL+IC

19

LGCGAHSTVSLVIC

32

24.1%

NZ_CP080519.1:

Lanthipeptides: K1T34_RS20225_lanthipeptide

7

STLSLLICW

15

STLSLL C

21

STLSLLTCH

29

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.39 out of 19.26

Confidence: strong

Additional weak hits (10):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 24.02 out of 30.22

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.90 out of 18.57

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.68 out of 30.25

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.92 out of 31.73

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.31 out of 29.25

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.35 out of 30.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.93 out of 27.78

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.27 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.80 out of 24.78

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.96 out of 29.25

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

MZC64_RS44570 - Class III

Cleavage pHMM score: 1.80

RODEO score: 0

Monoisotopic mass: 1538.9 Da

Molecular weight: 1539.9 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1557.9 Da N = 1; 1575.9 Da N = 2

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein

NZ_JALMDK010000023.1 - Region 1 - NRP-metallophore,NRPS

Download region GenBank file

Download region SVG

Location: 22,958 - 99,518 nt. (total: 76,561 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

MZC64_RS45120: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

MZC64_RS45185: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

MZC64_RS45335: lysine/ornithine N-monooxygenase

smcogs: SMCOG1080: lysine/ornithine N-monooxygenase

MZC64_RS45350: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Hydrolases: amidase

MZC64_RS45345: amidase

smcogs: SMCOG1105: amidase

Lyases: cyclase

MZC64_RS45385: diguanylate cyclase

smcogs: SMCOG1025: diguanylate cyclase

Isomerases: isomerase

MZC64_RS45340: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase

MZC64_RS45215: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

MZC64_RS45220: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

MZC64_RS45225: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

MZC64_RS45230: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

MZC64_RS45265: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002075.3	0.85	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0000342.4	0.84	NRPS	enniatin	Fusarium equiseti
BGC0001825.4	0.82	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001833.4	0.77	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.77	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.74	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001133.4	0.73	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.73	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0000465.4	0.72	NRPS	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.71	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0000342.4	0.73	NRPS	enniatin	Fusarium equiseti
BGC0001825.4	0.73	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001833.4	0.71	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.71	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001133.4	0.69	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.69	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0000465.4	0.69	NRPS	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.36 out of 19.26

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 26.57 out of 36.53

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 27.61 out of 36.53

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 28.57 out of 36.53

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 29.86 out of 36.53

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.44 out of 23.79

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 29.86 out of 36.53

Confidence: medium

Additional weak hits (17):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.88 out of 26.85

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.63 out of 30.22

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.58 out of 21.55

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.19 out of 18.57

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.47 out of 26.33

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.56 out of 21.55

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.36 out of 28.19

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.09 out of 23.43

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.54 out of 39.62

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.92 out of 25.75

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.98 out of 27.78

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.38 out of 20.97

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 22.65 out of 36.53

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 23.98 out of 36.53

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 20.29 out of 27.04

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 25.29 out of 36.53

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 20.55 out of 29.25

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 22957 - 99518:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser) + (X - D-X) + (NMe-OH-Orn - OH-Orn)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MZC64_RS45215: X

AMP-binding (37..442): X

nrpys: Sal

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: NASLFPLACPGGALQVGGAQQVFGMAEGLLNYTR
Nearest Stachelhaus code(s):: Sal PLPAQGVLNK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

MZC64_RS45220: Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (553..931): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: TDLSFDLSVFDGNCLLGGDVGLGGTTETAIHSTV
Nearest Stachelhaus code(s):: Ser DLFNLGLIHK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

MZC64_RS45225: X - X

AMP-binding (500..889): X

nrpys: bAla, me-bAla

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: RWGSFIDGSTEGTVITSGEVNLYGCSEAAGDSLA
Nearest Stachelhaus code(s):: me-bAla LDTTVSLGDK (76% 8Å match); bAla VDTVISLGDK (59% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1541..1946): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LWLTFDVSGWEWHFFCGGEYNMYGPAEATIDVTF
Nearest Stachelhaus code(s):: Dab or Asp DVWHFSLIDK (76% 8Å match); Glu DVWHFGRINK (65% 8Å match)
Stachelhaus code match:: 80% (moderate)

MZC64_RS45230: OH-Orn - OH-Orn

Search NORINE for peptide: strict or relaxed

AMP-binding (869..1284): OH-Orn

nrpys: OH-Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: MNHVFDMSWEEWNVLLGGEYNLYGPTEYTINTLG
Nearest Stachelhaus code(s):: OH-Orn DMENLGLINK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2347..2765): OH-Orn

nrpys: OH-Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: MNHVFDMSWEEWNVLLGGEYNLYGPTEYTINTLG
Nearest Stachelhaus code(s):: OH-Orn DMENLGLINK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

MZC64_RS45265: X

AMP-binding (31..490): X

nrpys: Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: NTLPAEGLLLGASAFVDGARCGYGP----VGAAA
Nearest Stachelhaus code(s):: Ant LGGSFAGLGK (26% 8Å match)
Stachelhaus code match:: 60% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
DmdR1	Iron(II)-dependent repressor
IolR	Regulator of the inositol catabolism

NZ_JALMDK010000025.1 - Region 1 - lassopeptide

Download region GenBank file

Download region SVG

Location: 40,517 - 62,816 nt. (total: 22,300 nt) Show pHMM detection rules used

Region on contig edge.

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lasso peptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001307.3	0.50	ribosomal	chaxapeptin	Streptomyces leeuwenhoekii
BGC0002006.2	0.49	ribosomal	albusnodin	Streptomyces albus
BGC0000575.5	0.48	ribosomal	lariatin	Rhodococcus jostii
BGC0001549.3	0.48	ribosomal	citrulassin B	Streptomyces aurantiacus JA 4570
BGC0001551.4	0.47	ribosomal	citrulassin E	Streptomyces glaucescens
BGC0002064.2	0.44	ribosomal	felipeptin A2, felipeptin A1	Amycolatopsis sp. YIM 10
BGC0002425.3	0.41	ribosomal	stlassin	Streptomyces sp.
BGC0001435.4	0.28	NRPS, PKS	ikarugamycin	Streptomyces sp. ZJ306
BGC0000197.5	0.25	PKS, saccharide	aranciamycin	Streptomyces echinatus
BGC0000213.4	0.23	PKS	colabomycin E	Streptomyces aureus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001307.3	0.50	ribosomal	chaxapeptin	Streptomyces leeuwenhoekii
BGC0002006.2	0.49	ribosomal	albusnodin	Streptomyces albus
BGC0000575.5	0.48	ribosomal	lariatin	Rhodococcus jostii
BGC0001549.3	0.48	ribosomal	citrulassin B	Streptomyces aurantiacus JA 4570
BGC0001551.4	0.47	ribosomal	citrulassin E	Streptomyces glaucescens
BGC0002064.2	0.44	ribosomal	felipeptin A2, felipeptin A1	Amycolatopsis sp. YIM 10
BGC0002425.3	0.41	ribosomal	stlassin	Streptomyces sp.
BGC0001435.4	0.28	NRPS, PKS	ikarugamycin	Streptomyces sp. ZJ306
BGC0000197.5	0.25	PKS, saccharide	aranciamycin	Streptomyces echinatus
BGC0000213.4	0.23	PKS	colabomycin E	Streptomyces aureus

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lasso peptide predictions

Lassopeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.83 out of 20.47

Confidence: medium

Additional weak hits (2):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.77 out of 26.85

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.52 out of 29.65

Confidence: weak

Lasso peptides

RREFinder

TFBS Finder

Core peptide predictions

No core peptides found.

RRE predictions

: RRE type: Stand_Alone_Lasso_RRE; Location: 4 - 75; Score: 35.9

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems

NZ_JALMDK010000027.1 - Region 1 - NAPAA,lanthipeptide-class-ii

Download region GenBank file

Download region SVG

Location: 26,063 - 62,048 nt. (total: 35,986 nt) Show pHMM detection rules used

Region on contig edge.

NAPAA: (AMP-binding and TIGR02353)
lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acetyltransferase

MZC64_RS46540: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Lyases: cyclase

MZC64_RS46640: diguanylate cyclase

smcogs: SMCOG1025: diguanylate cyclase

MZC64_RS46655: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Ligases: ligase

MZC64_RS46600: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002174.2	0.48	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0000525.5	0.36	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0002704.2	0.32	ribosomal	archalan β	Halorussus salinus
BGC0002316.2	0.27	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0002135.2	0.26	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000516.4	0.26	ribosomal	geobacillin II	Geobacillus thermodenitrificans
BGC0001641.4	0.26	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.25	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.25	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0000527.5	0.25	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002174.2	0.78	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0002135.2	0.60	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001641.4	0.59	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.59	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.59	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001268.4	0.55	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0002704.2	0.47	ribosomal	archalan β	Halorussus salinus
BGC0002518.3	0.46	NRPS	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0002171.2	0.46	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger ATCC 1015

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for MZC64_RS46655

MZC64_RS46660

Leader:

MSKHIQTPELHDFTEPVGALRLLPELNFGARAVALTRLALPVGLLAVGFFGSAAGA

Core:

PVDESTAAGITTCCPPVTL

Core with dehydrations:

PVDEDhaDhbAAGIDhbDhbCysCysPPVDhbL

antiSMASH-DB 4.0 matches for MZC64_RS46660

100.0%

NZ_JALMDK010000027.1:

Lanthipeptides: MZC64_RS46660_lanthipeptide

1

PVDESTAAGITTCCPPVTL

19

PVDESTAAGITTCCPPVTL

1

PVDESTAAGITTCCPPVTL

19

36.8%

NZ_CP116936.1:

Lanthipeptides: PCA76_RS13015_lanthipeptide

8

AGITTCCPPVTL

19

A +TTCCP L

1

APLTTCCPTFAL

12

35.0%

NZ_CP022961.1:

Lanthipeptides: CIK06_RS16610_lanthipeptide

8

AGITTCCPPVTL

19

G TTCCPP +

9

GGSTTCCPPSAM

20

with 5 more sequence matches

31.8%

NZ_FOTE01000003.1:

Lassopeptides: BM210_RS34275_lassopeptide

3

DESTAAGITTCCPPVT

18

DE+ AGI + P T

6

DEAYIAGIRSKLPRFT

21

25.6%

NC_017803.1:

Lanthipeptides: ACPL_RS19820_lanthipeptide

6

TAAGI--TTCCPP

16

TA GI TTCCPP

23

TAFGIASTTCCPP

35

25.0%

NZ_ALVU02000002.1and 1 other:

NZ_ALVU02000002.1 Lanthipeptides: SYN7509_RS28800_lanthipeptide

Lanthipeptides: SYN7509_RS28800_lanthipeptide

3

DESTAAGITTCCPPV

17

DES +GI C P+

8

DESCNSGIIACTLPL

22

24.0%

NZ_FOGI01000013.1and 2 others:

NZ_JAMTCR010000003.1 Lanthipeptides: LV79_RS16640_lanthipeptideNZ_RCDD01000003.1 Lanthipeptides: CLV68_RS20325_lanthipeptide

Lanthipeptides: BMY07_RS27390_lanthipeptide

9

GITTCCP

15

G TTCCP

15

GSTTCCP

21

21.1%

NZ_LISX01000002.1and 3 others:

NZ_LISX01000002.1 Lanthipeptides: allorf_740642_740746_lanthipeptideNZ_LISX01000002.1 Lanthipeptides: allorf_740642_740746_lanthipeptideNZ_LISX01000002.1 Lanthipeptides: allorf_740642_740746_lanthipeptide

Lanthipeptides: allorf_740642_740746_lanthipeptide

1

PVDESTAAGITTC

13

P+D+ A +++C

1

PIDDQEAVSLSSC

13

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (5):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.88 out of 30.25

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.16 out of 27.55

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.15 out of 30.25

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.05 out of 29.65

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.52 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptides

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Core peptide predictions

MZC64_RS46660 - Class II

Cleavage pHMM score: -6.10

RODEO score: 0

Monoisotopic mass: 1783.9 Da

Molecular weight: 1785.0 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 1803.0 Da N = 1; 1821.1 Da N = 2; 1839.1 Da N = 3

Predicted core structure(s)

For candidate cluster 1, location 26062 - 62048:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 26062 - 59917:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MZC64_RS46600: X

AMP-binding (22..393): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAELFDASCEELSTIFGGEWNTYGPTEATVVACA
Nearest Stachelhaus code(s):: Lys DAEDIGTVVK (71% 8Å match); Gln or Tyr DAESVGTVVK (65% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator

NZ_JALMDK010000002.1 - Region 1 - azole-containing-RiPP

Download region GenBank file

Download region SVG

Location: 74,384 - 96,722 nt. (total: 22,339 nt) Show pHMM detection rules used

azole-containing-RiPP: ((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Azole RiPPs

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

MZC64_RS06795: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

MZC64_RS06800: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Transferases: acetyltransferase aminotransferase

MZC64_RS06785: aminotransferase class V

smcogs: SMCOG1139: aminotransferase class V

MZC64_RS06805: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

MZC64_RS06810: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002115.3	0.66	ribosomal	streptamidine	Streptomyces albidoflavus J1074
BGC0002073.3	0.57	NRPS	dehydroxynocardamine	Corynebacterium propinquum
BGC0000772.5	0.47	saccharide	lipopolysaccharide	Brucella melitensis
BGC0000947.5	0.46	other (other)	vibrioferrin	Vibrio alginolyticus
BGC0000888.5	0.45	other (other)	bacilysin	Bacillus sp. CS93
BGC0002683.2	0.44	NRPS	schizokinen	Nostoc sp. PCC 7120 = FACHB-418
BGC0001870.3	0.44	other (other)	putrebactin, avaroferrin	Xenorhabdus budapestensis
BGC0000231.5	0.44	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0002476.2	0.44	NRPS	enterobactin	Escherichia coli str. K-12 substr. MG1655
BGC0000708.5	0.43	saccharide	lividomycin	Streptomyces lividus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002115.3	0.66	ribosomal	streptamidine	Streptomyces albidoflavus J1074
BGC0002073.3	0.57	NRPS	dehydroxynocardamine	Corynebacterium propinquum
BGC0000772.5	0.47	saccharide	lipopolysaccharide	Brucella melitensis
BGC0000947.5	0.46	other (other)	vibrioferrin	Vibrio alginolyticus
BGC0000888.5	0.45	other (other)	bacilysin	Bacillus sp. CS93
BGC0002683.2	0.44	NRPS	schizokinen	Nostoc sp. PCC 7120 = FACHB-418
BGC0001870.3	0.44	other (other)	putrebactin, avaroferrin	Xenorhabdus budapestensis
BGC0000231.5	0.44	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0002476.2	0.44	NRPS	enterobactin	Escherichia coli str. K-12 substr. MG1655
BGC0000708.5	0.43	saccharide	lividomycin	Streptomyces lividus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 25.54 out of 29.38

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.37 out of 20.47

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 21.63 out of 28.19

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.98 out of 28.19

Confidence: medium

Additional weak hits (14):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 20.05 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.29 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.73 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.92 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 22.07 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 22.37 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.72 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 19.51 out of 28.14

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.61 out of 20.97

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.30 out of 27.78

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.69 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.93 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.69 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.35 out of 28.19

Confidence: weak

(Thi)azol(in)e containing peptide predictions

No core peptides found.

TFBS Finder

Azole RiPPs

TFBS definitions

Regulator	Description
FuR variant 1	Ferric Uptake Regulator
VqsM	Regulator of quorum-sensing signalling systems
ZuR variant 1	Zinc-responsive repressor

Core peptide predictions

No core peptides found.

NZ_JALMDK010000002.1 - Region 2 - CDPS

Download region GenBank file

Download region SVG

Location: 638,957 - 659,652 nt. (total: 20,696 nt) Show pHMM detection rules used

CDPS: CDPS

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: phosphatase

MZC64_RS09510: inositol monophosphatase

smcogs: SMCOG1207: inositol monophosphatase

Lyases: cyclase

MZC64_RS09575: diguanylate cyclase

smcogs: SMCOG1025: diguanylate cyclase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000851.5	0.50	other (other)	albonoursin	Streptomyces noursei ATCC 11455
BGC0001986.3	0.48	other (other)	purincyclamide	Streptomyces chrestomyceticus
BGC0000878.5	0.36	other (other)	puromycin	Streptomyces alboniger
BGC0001824.4	0.36	NRPS	xefoampeptides A-G	Xenorhabdus beddingii
BGC0000285.5	0.35	PKS, saccharide	flaviolin, flaviolin rhamnoside, 3,3'-diflaviolin	Saccharopolyspora erythraea
BGC0001861.4	0.35	ribosomal	bacillicin BAG2O	Bacillus cereus BAG2O-1
BGC0001863.4	0.34	ribosomal, terpene	bacillicn CER074	Bacillus mycoides
BGC0002316.2	0.33	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0000528.5	0.32	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002346.3	0.31	ribosomal	kyamicin	Saccharopolyspora sp.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000851.5	0.50	other (other)	albonoursin	Streptomyces noursei ATCC 11455
BGC0001986.3	0.48	other (other)	purincyclamide	Streptomyces chrestomyceticus
BGC0000878.5	0.36	other (other)	puromycin	Streptomyces alboniger
BGC0001824.4	0.36	NRPS	xefoampeptides A-G	Xenorhabdus beddingii
BGC0000285.5	0.35	PKS, saccharide	flaviolin, flaviolin rhamnoside, 3,3'-diflaviolin	Saccharopolyspora erythraea
BGC0001861.4	0.35	ribosomal	bacillicin BAG2O	Bacillus cereus BAG2O-1
BGC0001863.4	0.34	ribosomal, terpene	bacillicn CER074	Bacillus mycoides
BGC0002316.2	0.33	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0000528.5	0.32	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002346.3	0.31	ribosomal	kyamicin	Saccharopolyspora sp.

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.25 out of 20.47

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.25 out of 20.47

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.83 out of 28.19

Confidence: medium

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 20.33 out of 23.43

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 20.59 out of 28.19

Confidence: medium

Additional weak hits (19):

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.69 out of 29.65

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.21 out of 23.43

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.88 out of 29.38

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.82 out of 25.88

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 22.53 out of 33.73

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 19.15 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.70 out of 26.06

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.08 out of 30.25

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.76 out of 30.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.52 out of 27.78

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.05 out of 19.26

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.73 out of 27.78

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.13 out of 25.75

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.36 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.42 out of 29.38

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.35 out of 27.18

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.04 out of 28.19

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.50 out of 30.98

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.39 out of 28.19

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
BldD	Development and antibiotic global regulator
VqsM	Regulator of quorum-sensing signalling systems
ZuR variant 1	Zinc-responsive repressor

NZ_JALMDK010000002.1 - Region 3 - lanthipeptide-class-i

Download region GenBank file

Download region SVG

Location: 743,846 - 770,333 nt. (total: 26,488 nt) Show pHMM detection rules used

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: glycosyltransferase

MZC64_RS10080: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

Hydrolases: hydrolase

MZC64_RS10155: HAD-superfamily hydrolase, subfamily IA, variant

smcogs: SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant

Lyases: cyclase dehydratase

MZC64_RS10130: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

MZC64_RS10135: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.75	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0002316.2	0.47	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0001289.5	0.46	ribosomal	penisin	Paenibacillus ehimensis
BGC0002635.2	0.46	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0002120.3	0.45	ribosomal	nocathioamide A	Nocardia terpenica IFM 0706
BGC0000881.3	0.45	other (other)	toyocamycin	Streptomyces ahygroscopicus subsp. wuzhouensis
BGC0001808.5	0.45	other (other)	toyocamycin	Streptomyces diastatochromogenes
BGC0002354.4	0.42	other (other)	huimycin	Kutzneria albida DSM 43870
BGC0000544.4	0.42	ribosomal	planosporicin	Planomonospora alba
BGC0000529.5	0.40	ribosomal	microbisporicin A2	Microbispora corallina

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.75	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0002316.2	0.47	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0001289.5	0.46	ribosomal	penisin	Paenibacillus ehimensis
BGC0002635.2	0.46	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0002120.3	0.45	ribosomal	nocathioamide A	Nocardia terpenica IFM 0706
BGC0000881.3	0.45	other (other)	toyocamycin	Streptomyces ahygroscopicus subsp. wuzhouensis
BGC0001808.5	0.45	other (other)	toyocamycin	Streptomyces diastatochromogenes
BGC0002354.4	0.42	other (other)	huimycin	Kutzneria albida DSM 43870
BGC0000544.4	0.42	ribosomal	planosporicin	Planomonospora alba
BGC0000529.5	0.40	ribosomal	microbisporicin A2	Microbispora corallina

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for MZC64_RS10130

MZC64_RS10125

Leader:

MTVQMEWSPAAVATPAAEVEMGSEFDLDITLVEVVDPA

Core:

HLINMTDDGCGTTCEKSTCISAA

Core with dehydrations:

HLINMDhbDDGCysGDhbDhbCysEKDhaDhbCysIDhaAA

antiSMASH-DB 4.0 matches for MZC64_RS10125

100.0%

NZ_CM001439.1and 3 others:

NZ_CP015163.1 Lanthipeptides: A4R43_RS24965_lanthipeptideNZ_FOKG01000036.1 Lanthipeptides: BM199_RS31630_lanthipeptideNZ_JALMDK010000002.1 Lanthipeptides: MZC64_RS10125_lanthipeptide

Lanthipeptides: SACMADRAFT_RS28825_lanthipeptide

1

HLINMTDDGCGTTCEKSTCISAA

23

HLINMTDDGCGTTCEKSTCISAA

1

HLINMTDDGCGTTCEKSTCISAA

23

95.7%

NZ_BBOJ01000005.1and 2 others:

NZ_CM001439.1 Lanthipeptides: SACMADRAFT_RS29465_lanthipeptideNZ_CM001439.1 Lanthipeptides: SACMADRAFT_RS29465_lanthipeptide

Lanthipeptides: AOM45_RS49405_lanthipeptide

1

HLINMTDDGCGTTCEKSTCISAA

23

HLINMTDDGCG TCEKSTCISAA

1

HLINMTDDGCGHTCEKSTCISAA

23

95.7%

NZ_SWMS01000027.1:

Lanthipeptides: FCN18_RS31945_lanthipeptide

1

HLINMTDDGCGTTCEKSTCISAA

23

HLINMTDDGCG TCEKSTCISAA

1

HLINMTDDGCGQTCEKSTCISAA

23

with 177 more sequence matches

Only the first 10 are displayed here.

91.3%

NZ_FOUP01000006.1:

Lanthipeptides: BM016_RS18395_lanthipeptide

1

HLINMTDDGCGTTCEKSTCISAA

23

HLINMTDDGCG TCEKSTCIS+A

1

HLINMTDDGCGHTCEKSTCISSA

23

60.9%

NZ_AZXF01000017.1:

Lanthipeptides: B042_RS24865_lanthipeptide

3

INMTDDGCGTTCEKSTCISAA

23

IN+TDDGCGTTC STC+++

1

INLTDDGCGTTC-PSTCVTSV

20

60.9%

NZ_JAMXJO010000001.1:

Lanthipeptides: NGM33_RS06840_lanthipeptide

2

LINMTDDGCGTTCEKSTCISAA

23

LIN+TDDGCG++C S SAA

1

LINLTDDGCGSSCPNSCTTSAA

22

60.9%

NZ_QOIL01000028.1:

Lanthipeptides: DQ384_RS35715_lanthipeptide

2

LINMTDDGCGTTCEKSTCISA

22

LIN+TDDGCG+TC S C +A

1

LINLTDDGCGSTC-ASPCATA

20

56.5%

NC_007777.1and 1 other:

NC_007777.1 Lanthipeptides: FRANCCI3_RS10225_lanthipeptide

Lanthipeptides: FRANCCI3_RS10225_lanthipeptide

1

HLINMTDDGCGTTCEKS

17

LI MTDDGCGTTCE +

1

RLIRMTDDGCGTTCETA

17

56.5%

NZ_CP044407.1:

Lanthipeptides: F7P10_RS18930_lanthipeptide

2

LINMTDDGCGTTCEKSTCISAA

23

L+N+TDDGC +TC CIS A

2

LVNVTDDGCTSTC--GACISNA

21

56.5%

NZ_KB906876.1and 1 other:

NZ_VWVS01000014.1 Lanthipeptides: FYW99_RS19210_lanthipeptide

Lanthipeptides: G011_RS31385_lanthipeptide

2

LINMTDDGCGTTCEKSTCISA

22

LIN+TDDGCGT+C +TC+++

1

LINLTDDGCGTSC-PTTCVTS

20

56.5%

NZ_RBWU01000008.1:

Lanthipeptides: BZB76_RS31240_lanthipeptide

3

INMTDDGCGTTCEKSTCISAA

23

+N+TDDGC TC +S C SAA

1

VNLTDDGCNPTCPES-CTSAA

20

56.0%

NZ_CP031264.1and 1 other:

NZ_CP031264.1 Lanthipeptides: C7M71_RS17720_lanthipeptide

Lanthipeptides: C7M71_RS17720_lanthipeptide

2

LINMTDDGCGTTCE----KSTCISA

22

L+N T DGCG+TC+ STCIS

1

LLNDTGDGCGSTCQSACSNSTCISG

25

52.2%

NZ_BCBX01000061.1and 1 other:

NZ_BCBX01000061.1 Lanthipeptides: AP234_RS35640_lanthipeptide

Lanthipeptides: AP234_RS35640_lanthipeptide

1

HLINMTDDGCGTTCEKSTCIS

21

LI MTDDGC +TC + C+S

1

QLIRMTDDGCNSTC-ATACVS

20

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (6):

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.50 out of 25.75

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.77 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.58 out of 28.14

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.14 out of 30.22

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.27 out of 30.22

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.65 out of 35.63

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

MZC64_RS10125 - Class I

Cleavage pHMM score: 1.70

RODEO score: 22

Monoisotopic mass: 2216.0 Da

Molecular weight: 2217.5 Da

Number of bridges: 2

Additional modifications: AviCys

Alternative weights: (assuming N unmodified Ser/Thr residues); 2235.5 Da N = 1; 2253.6 Da N = 2; 2271.6 Da N = 3

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 161 - 253; Score: 67.2

TFBS definitions

Regulator	Description

NZ_JALMDK010000031.1 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 22,999 nt. (total: 22,999 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

MZC64_RS47205: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

MZC64_RS47215: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Ligases: synthase

MZC64_RS47210: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001244.3	0.73	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.70	PKS	oronofacic acid	Pseudomonas syringae
BGC0001276.3	0.64	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002192.3	0.62	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.62	PKS	ACT-Toxin II	Alternaria alternata
BGC0001164.3	0.61	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.61	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001257.3	0.61	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001244.3	0.73	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.70	PKS	oronofacic acid	Pseudomonas syringae
BGC0001276.3	0.64	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002192.3	0.62	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.62	PKS	ACT-Toxin II	Alternaria alternata
BGC0001164.3	0.61	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.61	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001257.3	0.61	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 22999:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk - redmal) + (Me-ccmal)
Non-colinear ordering:: MZC64_RS47210 + MZC64_RS47205

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MZC64_RS47205: mmal - mal

PKS_AT (550..844): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 191.5
Ethylmalonyl-CoA: 190.6
Methoxymalonyl-CoA: 148.7
2-Methylbutyryl-CoA: 114.0
trans-1,2-CPDA: 108.2
Propionyl-CoA: 108.2
Malonyl-CoA: 105.4
CHC-CoA: 81.8
Isobutyryl-CoA: 64.1
Benzoyl-CoA: 56.9
Acetyl-CoA: 32.4
inactive: 29.7
fatty_acid: 27.2
3-Methylbutyryl-CoA: 17.3

PKS_AT (2235..2459): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 75.0%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 142.0
inactive: 74.1
Methoxymalonyl-CoA: 40.8
Methylmalonyl-CoA: 26.4
Isobutyryl-CoA: 17.6
Acetyl-CoA: 12.5
Propionyl-CoA: 10.7
fatty_acid: 10.1
Benzoyl-CoA: 9.9
trans-1,2-CPDA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

MZC64_RS47210: pk - mal

PKS_AT (562..857): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 195.5
Methylmalonyl-CoA: 194.3
Methoxymalonyl-CoA: 153.8
Propionyl-CoA: 117.8
2-Methylbutyryl-CoA: 117.5
trans-1,2-CPDA: 114.9
Malonyl-CoA: 106.0
CHC-CoA: 82.9
Isobutyryl-CoA: 69.2
Benzoyl-CoA: 53.6
Acetyl-CoA: 38.5
inactive: 30.8
fatty_acid: 25.5
3-Methylbutyryl-CoA: 25.3

PKS_AT (2600..2874): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 172.0
inactive: 85.1
Methoxymalonyl-CoA: 55.2
Methylmalonyl-CoA: 36.6
Isobutyryl-CoA: 22.3
Propionyl-CoA: 17.8
fatty_acid: 17.5
Ethylmalonyl-CoA: 11.6
Acetyl-CoA: 10.6
Benzoyl-CoA: 10.0
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

NZ_JALMDK010000003.1 - Region 1 - terpene

Download region GenBank file

Download region SVG

Location: 26,837 - 49,218 nt. (total: 22,382 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

MZC64_RS11450: alpha/beta hydrolase domain-containing protein

smcogs: SMCOG1066: alpha/beta hydrolase domain-containing protein

Lyases: cyclase

MZC64_RS11435: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.81	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0002073.3	0.48	NRPS	dehydroxynocardamine	Corynebacterium propinquum
BGC0002260.3	0.45	terpene	trichobrasilenol, xylarenic acid B, brasilane A, brasilane F, brasilane E, brasilane D	Annulohypoxylon truncatum
BGC0001870.3	0.44	other (other)	putrebactin, avaroferrin	Xenorhabdus budapestensis
BGC0002306.2	0.43	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0000551.5	0.42	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0001211.5	0.42	NRPS	albachelin	Amycolatopsis alba DSM 44262
BGC0000558.5	0.42	ribosomal	sublancin 168	Bacillus subtilis subsp. subtilis str. 168
BGC0000501.3	0.42	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000519.4	0.42	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.81	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0002073.3	0.48	NRPS	dehydroxynocardamine	Corynebacterium propinquum
BGC0002260.3	0.45	terpene	trichobrasilenol, xylarenic acid B, brasilane A, brasilane F, brasilane E, brasilane D	Annulohypoxylon truncatum
BGC0001870.3	0.44	other (other)	putrebactin, avaroferrin	Xenorhabdus budapestensis
BGC0002306.2	0.43	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0000551.5	0.42	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0001211.5	0.42	NRPS	albachelin	Amycolatopsis alba DSM 44262
BGC0000558.5	0.42	ribosomal	sublancin 168	Bacillus subtilis subsp. subtilis str. 168
BGC0000501.3	0.42	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000519.4	0.42	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene		germacradienol	C15	C1-C10	OH
sesquiterpene		geosmin	C12	C1-C10	OH

Genes with terpene domains:

MZC64_RS11435: T1TS + T1TS

Domain 1 (1 - 328): Type 1 terpene synthase

Subtype: Geosmin synthase, N terminal domain

Reactions:: FPP → germacradienol

Domain 2 (329 - 706): Type 1 terpene synthase

Subtype: Geosmin synthase, C terminal domain

Reactions:: germacradienol → geosmin

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.50 out of 20.47

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 20.80 out of 23.79

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.37 out of 23.79

Confidence: medium

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.70 out of 26.85

Confidence: medium

Additional weak hits (5):

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 24.08 out of 36.53

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.73 out of 29.38

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.43 out of 26.85

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.23 out of 28.19

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.64 out of 29.65

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
RutR	Regulator of the pyrimidine and purine metabolism
VqsM	Regulator of quorum-sensing signalling systems

NZ_JALMDK010000003.1 - Region 2 - terpene-precursor

Download region GenBank file

Download region SVG

Location: 197,322 - 218,428 nt. (total: 21,107 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

MZC64_RS12180: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

MZC64_RS12195: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Hydrolases: hydrolase

MZC64_RS12170: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Ligases: synthetase

MZC64_RS12185: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000664.5	0.49	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.49	terpene	isorenieratene	Streptomyces argillaceus
BGC0001757.3	0.49	other (other)	rhabduscin	Xenorhabdus nematophila ATCC 19061
BGC0000923.3	0.48	other (other)	paerucumarin	Pseudomonas aeruginosa PAO1
BGC0000649.3	0.48	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000633.3	0.47	terpene	carotenoid	Streptomyces avermitilis
BGC0001227.3	0.47	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000677.5	0.42	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000648.3	0.42	terpene	carotenoid	Myxococcus xanthus
BGC0000843.5	0.40	other (non-nrp beta-lactam)	alanylclavam, 2-hydroxymethylclavam, 2-formyloxymethylclavam, clavam-2-carboxylate	Streptomyces clavuligerus ATCC 27064

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000664.5	0.49	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.49	terpene	isorenieratene	Streptomyces argillaceus
BGC0001757.3	0.49	other (other)	rhabduscin	Xenorhabdus nematophila ATCC 19061
BGC0000923.3	0.48	other (other)	paerucumarin	Pseudomonas aeruginosa PAO1
BGC0000649.3	0.48	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000633.3	0.47	terpene	carotenoid	Streptomyces avermitilis
BGC0001227.3	0.47	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000677.5	0.42	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000648.3	0.42	terpene	carotenoid	Myxococcus xanthus
BGC0000843.5	0.40	other (non-nrp beta-lactam)	alanylclavam, 2-hydroxymethylclavam, 2-formyloxymethylclavam, clavam-2-carboxylate	Streptomyces clavuligerus ATCC 27064

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

MZC64_RS12185: PT_FPPS_like

Domain 1 (43 - 367): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.90 out of 23.79

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 20.57 out of 25.31

Confidence: medium

Additional weak hits (5):

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.83 out of 29.25

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.71 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.10 out of 29.38

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.76 out of 27.55

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.77 out of 35.63

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
CatR	H2O2-responsive repressor
IolR	Regulator of the inositol catabolism

NZ_JALMDK010000003.1 - Region 3 - lanthipeptide-class-i

Download region GenBank file

Download region SVG

Location: 608,197 - 632,751 nt. (total: 24,555 nt) Show pHMM detection rules used

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

MZC64_RS14195: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

Hydrolases: deacetylase

MZC64_RS14135: acetylornithine deacetylase

smcogs: SMCOG1247: acetylornithine deacetylase

Lyases: cyclase dehydratase

MZC64_RS14180: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

MZC64_RS14185: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.75	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0000542.3	0.58	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000541.5	0.58	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0001289.5	0.57	ribosomal	penisin	Paenibacillus ehimensis
BGC0002640.2	0.56	ribosomal	thalassomonasin A, thalassomonasin B	Thalassomonas actiniarum
BGC0002635.2	0.56	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0000544.4	0.53	ribosomal	planosporicin	Planomonospora alba
BGC0000515.4	0.53	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2
BGC0000506.4	0.52	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0001727.3	0.51	ribosomal	paenilan	Paenibacillus polymyxa E681

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.75	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0000542.3	0.58	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000541.5	0.58	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0001289.5	0.57	ribosomal	penisin	Paenibacillus ehimensis
BGC0002640.2	0.56	ribosomal	thalassomonasin A, thalassomonasin B	Thalassomonas actiniarum
BGC0002635.2	0.56	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0000544.4	0.53	ribosomal	planosporicin	Planomonospora alba
BGC0000515.4	0.53	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2
BGC0000506.4	0.52	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0001727.3	0.51	ribosomal	paenilan	Paenibacillus polymyxa E681

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for MZC64_RS14185

MZC64_RS14175

Leader:

MDAFDLDVRITTPAGADGAAGPL

Core:

SIISKLACSRTPVCSKTTCQCSRTCTPLCTSGSTR

Core with dehydrations:

DhaIIDhaKLACysDhaRDhbPVCysDhaKDhbDhbCysQCysDhaRDhbCysDhbPLCysDhbDhaGDhaDhbR

antiSMASH-DB 4.0 matches for MZC64_RS14175

100.0%

NZ_JALMDK010000003.1:

Lanthipeptides: MZC64_RS14175_lanthipeptide

1

SIISKLACSRTPVCSKTTCQCSRTCTPLCTSGSTR

35

SIISKLACSRTPVCSKTTCQCSRTCTPLCTSGSTR

1

SIISKLACSRTPVCSKTTCQCSRTCTPLCTSGSTR

35

38.9%

NZ_JALMDK010000007.1:

Lanthipeptides: MZC64_RS28180_lanthipeptide

1

SIISKLACSRTPVCSKTTCQCSR

23

S+I++LAC+RTP+CS+ TCQC++

4

SVITRLACTRTPLCSRFTCQCTQ

26

34.9%

NZ_CP047318.1:

Lanthipeptides: GSY65_RS20765_lanthipeptide

3

ISKLACSRTPVCSKTTCQ------CSRTCTPLCTSG

32

IS++A +RT VC+K+ CQ C+ CT C+ G

9

ISQIA-TRT-VCTKSVCQVTSTCACTSVCTVFCSPG

42

with 7 more sequence matches

30.2%

NZ_JAAVVL010000001.1:

Lanthipeptides: GWK25_RS02255_lanthipeptide

2

IISKLACSRTPVCSKT-TCQCSRTCTPLCTSG

32

I +K C+++ VC T TC C+ CT C+ G

12

IATKTVCTKS-VCQVTSTCACTSVCTVFCSPG

42

28.6%

NZ_CP033464.1:

Lanthipeptides: EEL30_RS11035_lanthipeptide

1

SIISKLACSRTPVCSKTTCQ

20

S++S L C T CS T CQ

1

SVLSDLIC--TTFCSATFCQ

18

28.6%

NZ_CP099837.1:

Lanthipeptides: NE857_RS03345_lanthipeptide

16

KTTCQCSRTCTPLCTSGS

33

T+ CS TC CTSGS

5

DTSNGCSSTCPSACTSGS

22

26.8%

NZ_CP017248.1and 1 other:

NZ_CP071839.1 Lanthipeptides: S1361_RS32765_lanthipeptide

Lanthipeptides: BFF78_RS08045_lanthipeptide

13

VCSKTTCQ--CSRTCTPLCTSG

32

VC++ TC+ C+ CT +CTSG

12

VCTRVTCKANCTYVCTTVCTSG

33

25.6%

NZ_JALGBX010000020.1and 1 other:

NZ_JANAVF010000016.1 Lanthipeptides: NLX94_RS30150_lanthipeptide

Lanthipeptides: MTF69_RS28785_lanthipeptide

13

VCSKTTCQCSRT------CTPLCTSG

32

VC++ TCQ S+T CT C G

17

VCTRVTCQASKTCVCTAMCTVFCVGG

42

16.7%

NZ_KK853997.1and 1 other:

NZ_KK853997.1 Lanthipeptides: KCH_RS04815_lanthipeptide

Lanthipeptides: KCH_RS04815_lanthipeptide

21

CSRTCTPLCTSGS

33

C+R CT CT G

15

CTRNCTNGCTGGG

27

14.3%

NZ_SGWQ01000010.1:

Lassopeptides: EV193_RS25540_lassopeptide

6

LACSRTPVC

14

L C R P+C

11

LGCGRAPIC

19

MIBiG 4.0 matches for MZC64_RS14175

17.1%

BGC0000553.3 (SSGG_RS09925):

Lanthipeptide: SRO15-2212

15

SKTTCQCSRTCTPLCT

30

S C+ S LCT

6

SLLVCEYSSLSVVLCT

21

14.3%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

25

CTPLCTSGS

33

CTP C +G+

7

CTPGCKTGA

15

11.4%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

18

TCQCS

22

TC CS

25

TCHCS

29

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.90 out of 20.97

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.57 out of 28.19

Confidence: medium

Additional weak hits (10):

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 19.59 out of 33.73

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 20.51 out of 36.54

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 23.90 out of 36.54

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 19.28 out of 27.04

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.16 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.88 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.11 out of 29.38

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.23 out of 31.73

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.07 out of 36.54

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.37 out of 31.73

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

MZC64_RS14175 - Class I

Cleavage pHMM score: 4.40

RODEO score: 23

Monoisotopic mass: 3426.7 Da

Molecular weight: 3429.0 Da

Number of bridges: 6

Alternative weights: (assuming N unmodified Ser/Thr residues); 3447.0 Da N = 1; 3465.1 Da N = 2; 3483.1 Da N = 3; 3501.1 Da N = 4; 3519.1 Da N = 5; 3537.1 Da N = 6; 3555.2 Da N = 7; 3573.2 Da N = 8

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 157 - 246; Score: 45.7

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A
ZuR variant 1	Zinc-responsive repressor

NZ_JALMDK010000003.1 - Region 4 - terpene

Download region GenBank file

Download region SVG

Location: 820,734 - 841,789 nt. (total: 21,056 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

MZC64_RS15040: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

MZC64_RS15080: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Lyases: cyclase

MZC64_RS15070: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.76	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0001387.5	0.48	other (other)	nucleocidin	Streptomyces calvus
BGC0000563.4	0.42	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0001779.5	0.41	ribosomal	siamycin	Streptomyces nodosus
BGC0000486.4	0.40	ribosomal	butyrivibriocin AR10	Butyrivibrio fibrisolvens
BGC0001336.5	0.40	other (other)	erdasporine A, erdasporine B, erdasporine C	uncultured bacterium
BGC0001743.3	0.39	ribosomal	plantaricyclin	Lactobacillus plantarum
BGC0000526.5	0.39	ribosomal	macedocin	Streptococcus macedonicus
BGC0002669.2	0.39	ribosomal	TVA-YJ-2	Streptomyces sp. NRRL S-87
BGC0001778.5	0.38	other (other)	showdomycin	Streptomyces showdoensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.76	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0001387.5	0.48	other (other)	nucleocidin	Streptomyces calvus
BGC0000563.4	0.42	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0001779.5	0.41	ribosomal	siamycin	Streptomyces nodosus
BGC0000486.4	0.40	ribosomal	butyrivibriocin AR10	Butyrivibrio fibrisolvens
BGC0001336.5	0.40	other (other)	erdasporine A, erdasporine B, erdasporine C	uncultured bacterium
BGC0001743.3	0.39	ribosomal	plantaricyclin	Lactobacillus plantarum
BGC0000526.5	0.39	ribosomal	macedocin	Streptococcus macedonicus
BGC0002669.2	0.39	ribosomal	TVA-YJ-2	Streptomyces sp. NRRL S-87
BGC0001778.5	0.38	other (other)	showdomycin	Streptomyces showdoensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

No product predictions

Genes with terpene domains:

MZC64_RS15070: T1TS

Domain 1 (12 - 323): Type 1 terpene synthase

Subtype: unknown

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.58 out of 23.79

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.50 out of 20.47

Confidence: medium

Additional weak hits (5):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.51 out of 21.84

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.51 out of 21.84

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.69 out of 30.98

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 15.94 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.16 out of 33.14

Confidence: weak

Terpene

TFBS Finder

Glossary

No acronyms to display

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
IolR	Regulator of the inositol catabolism
VqsM	Regulator of quorum-sensing signalling systems

NZ_JALMDK010000003.1 - Region 5 - RRE-containing,other

Download region GenBank file

Download region SVG

Location: 855,885 - 897,234 nt. (total: 41,350 nt) Show pHMM detection rules used

other: (Neocarzinostat or bcpB or frbD or mitE or prnB or CaiA or bacilysin or orf2_PTase)
RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase reductase

MZC64_RS15245: alkyl hydroperoxide reductase subunit

smcogs: SMCOG1176: alkyl hydroperoxide reductase subunit

MZC64_RS15305: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

Transferases: glycosyltransferase methyltransferase

MZC64_RS15230: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

MZC64_RS15280: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

MZC64_RS15295: methyltransferase

smcogs: SMCOG1089: methyltransferase

Hydrolases: protease

MZC64_RS15215: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: lyase

MZC64_RS15290: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002573.2	0.40	other (other)	phosphoramidon	Streptomyces mozunensis
BGC0000585.5	0.32	ribosomal	microcin C7	Escherichia coli
BGC0000888.5	0.28	other (other)	bacilysin	Bacillus sp. CS93
BGC0001184.4	0.25	other (other)	bacilysin	Bacillus velezensis FZB42
BGC0000933.3	0.23	other (other)	xiamenmycin A	Streptomyces xiamenensis
BGC0000665.5	0.21	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0001366.5	0.21	PKS	BE-24566B, zunyimycin A	Streptomyces sp. FJS31-2
BGC0001513.3	0.21	PKS	anthrabenzoxocinone	Actinobacteria bacterium
BGC0002064.2	0.20	ribosomal	felipeptin A2, felipeptin A1	Amycolatopsis sp. YIM 10
BGC0000597.4	0.20	ribosomal	SWA-2138	Streptomyces sp. e14

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002573.2	0.57	other (other)	phosphoramidon	Streptomyces mozunensis
BGC0000585.5	0.47	ribosomal	microcin C7	Escherichia coli
BGC0000277.5	0.46	PKS	urdamycin	Streptomyces fradiae
BGC0000888.5	0.45	other (other)	bacilysin	Bacillus sp. CS93
BGC0001184.4	0.43	other (other)	bacilysin	Bacillus velezensis FZB42
BGC0000595.3	0.43	ribosomal	SCO-2138	Streptomyces coelicolor A3(2)
BGC0000902.4	0.42	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0001782.3	0.42	other (other)	spiroindimicin A, spiroindimicin B, spiroindimicin C, spiroindimicin D, indimicin A, indimicin B, indimicin C, indimicin D, indimicin E, lynamicin A, lynamicin D, lynamicin F, lynamicin G	Streptomyces sp. SCSIO 03032
BGC0000285.5	0.42	PKS, saccharide	flaviolin, flaviolin rhamnoside, 3,3'-diflaviolin	Saccharopolyspora erythraea
BGC0000019.5	0.41	PKS	angolamycin	Streptomyces eurythermus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.60 out of 19.26

Confidence: medium

Additional weak hits (10):

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.44 out of 20.97

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.49 out of 21.55

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.38 out of 21.55

Confidence: weak

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 24.43 out of 32.93

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.01 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.15 out of 27.78

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 18.83 out of 35.63

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 22.98 out of 36.53

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.46 out of 29.65

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.39 out of 32.93

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: Pantocin_Microcin_RRE; Location: 0 - 90; Score: 65.5

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein

NZ_JALMDK010000003.1 - Region 6 - sactipeptide

Download region GenBank file

Download region SVG

Location: 919,260 - 940,774 nt. (total: 21,515 nt) Show pHMM detection rules used

sactipeptide: (subtilosin or thuricin or TIGR04404 or Subtilosin_A or skfc)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Sactipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000601.4	0.48	ribosomal	sporulation killing factor	Bacillus subtilis subsp. subtilis str. 168
BGC0002306.2	0.47	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0002150.2	0.44	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.44	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0000623.3	0.43	ribosomal	plantaricin W α, plantaricin W β	Lactobacillus plantarum
BGC0001639.3	0.41	ribosomal	klebsidin	Klebsiella pneumoniae
BGC0000504.5	0.40	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0000500.5	0.40	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0000583.5	0.38	ribosomal	grisemycin	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000517.4	0.37	ribosomal	haloduracin β, haloduracin α	Bacillus halodurans C-125

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000601.4	0.48	ribosomal	sporulation killing factor	Bacillus subtilis subsp. subtilis str. 168
BGC0002306.2	0.47	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0002150.2	0.44	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.44	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0000623.3	0.43	ribosomal	plantaricin W α, plantaricin W β	Lactobacillus plantarum
BGC0001639.3	0.41	ribosomal	klebsidin	Klebsiella pneumoniae
BGC0000504.5	0.40	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0000500.5	0.40	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0000583.5	0.38	ribosomal	grisemycin	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000517.4	0.37	ribosomal	haloduracin β, haloduracin α	Bacillus halodurans C-125

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Sactipeptide predictions

Sactipeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 24.85 out of 30.12

Confidence: medium

Additional weak hits (11):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.37 out of 30.22

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.23 out of 21.55

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.81 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.33 out of 28.14

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.08 out of 21.55

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.31 out of 25.88

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.11 out of 25.75

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 24.27 out of 30.12

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.65 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.06 out of 28.19

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.84 out of 32.93

Confidence: weak

Sactipeptides

TFBS Finder

Core peptide predictions

No core peptides found.

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
OsdR	Development and stress management regulator

NZ_JALMDK010000003.1 - Region 7 - terpene

Download region GenBank file

Download region SVG

Location: 949,853 - 973,866 nt. (total: 24,014 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

MZC64_RS15600: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

MZC64_RS15625: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

MZC64_RS15645: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

MZC64_RS15660: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

MZC64_RS15665: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Ligases: synthetase

MZC64_RS15630: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001605.3	0.54	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0002631.2	0.54	ribosomal	Myxarylin	Pyxidicoccus fallax
BGC0002043.3	0.54	ribosomal	cittilin A, cittilin B	Myxococcus xanthus DK 1622
BGC0002347.3	0.53	other (other)	cyanogramide, cyanogramide B, cyanogramide C, cyanogramide D	Actinoalloteichus caeruleus WH1-2216-6
BGC0002397.2	0.52	terpene	astallatene	Arabidopsis thaliana
BGC0001529.5	0.51	other (other)	benzastatin J, 7-hydroxyl benzastatin B, 7-hydroxyl O-demethylbenzastatin A, JBIR-67, 7-hydroxyl benzastatin F, 7-hydroxyl O-demethylbenzastatin D, O-demethylvirantmycin, 7-hydroxyl benzastatin D, virantmycin, benzastatin K, methylbenzastatin K	Streptomyces sp. RI18
BGC0000677.5	0.51	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001310.5	0.51	PKS	naringenin	Streptomyces clavuligerus ATCC 27064
BGC0002400.2	0.49	terpene	boleracene	Brassica oleracea var. oleracea
BGC0001528.4	0.48	terpene	betaestacin I, betaestacin II	Phoma betae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001605.3	0.54	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0002631.2	0.54	ribosomal	Myxarylin	Pyxidicoccus fallax
BGC0002043.3	0.54	ribosomal	cittilin A, cittilin B	Myxococcus xanthus DK 1622
BGC0002347.3	0.53	other (other)	cyanogramide, cyanogramide B, cyanogramide C, cyanogramide D	Actinoalloteichus caeruleus WH1-2216-6
BGC0002397.2	0.52	terpene	astallatene	Arabidopsis thaliana
BGC0001529.5	0.51	other (other)	benzastatin J, 7-hydroxyl benzastatin B, 7-hydroxyl O-demethylbenzastatin A, JBIR-67, 7-hydroxyl benzastatin F, 7-hydroxyl O-demethylbenzastatin D, O-demethylvirantmycin, 7-hydroxyl benzastatin D, virantmycin, benzastatin K, methylbenzastatin K	Streptomyces sp. RI18
BGC0000677.5	0.51	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001310.5	0.51	PKS	naringenin	Streptomyces clavuligerus ATCC 27064
BGC0002400.2	0.49	terpene	boleracene	Brassica oleracea var. oleracea
BGC0001528.4	0.48	terpene	betaestacin I, betaestacin II	Phoma betae

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

MZC64_RS15620: T2TS

Domain 1 (277 - 556): Type 2 terpene synthase

Subtype: unknown

MZC64_RS15630: PT_FPPS_like

Domain 1 (37 - 308): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.79 out of 23.79

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.99 out of 20.47

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.11 out of 19.52

Confidence: medium

Additional weak hits (12):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.86 out of 26.85

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.12 out of 31.73

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.87 out of 24.78

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.51 out of 21.84

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.27 out of 25.75

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 23.65 out of 30.98

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.82 out of 18.57

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.41 out of 33.14

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 19.14 out of 22.40

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.66 out of 33.14

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.02 out of 32.93

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.21 out of 32.93

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
IolR	Regulator of the inositol catabolism
VqsM	Regulator of quorum-sensing signalling systems

NZ_JALMDK010000004.1 - Region 1 - lassopeptide

Download region GenBank file

Download region SVG

Location: 62,366 - 84,932 nt. (total: 22,567 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lasso peptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acetyltransferase

MZC64_RS16050: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

MZC64_RS16075: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Hydrolases: esterase hydrolase

MZC64_RS16065: thioesterase superfamily protein

smcogs: SMCOG1144: thioesterase superfamily protein

MZC64_RS16085: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002006.2	0.68	ribosomal	albusnodin	Streptomyces albus
BGC0001548.4	0.43	ribosomal	citrulassin A	Streptomyces albulus
BGC0000902.4	0.42	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0002318.2	0.42	ribosomal	canucin A, canucin B	Streptomyces canus
BGC0001552.3	0.41	ribosomal	citrulassin F	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001550.3	0.40	ribosomal	citrulassin D	Streptomyces katrae
BGC0002825.2	0.40	ribosomal	nocapeptin A	Nocardia terpenica IFM 0406
BGC0001551.4	0.39	ribosomal	citrulassin E	Streptomyces glaucescens
BGC0001889.2	0.39	ribosomal	fusilassin	Thermobifida fusca YX
BGC0000285.5	0.37	PKS, saccharide	flaviolin, flaviolin rhamnoside, 3,3'-diflaviolin	Saccharopolyspora erythraea

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002006.2	0.68	ribosomal	albusnodin	Streptomyces albus
BGC0001548.4	0.43	ribosomal	citrulassin A	Streptomyces albulus
BGC0000902.4	0.42	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0002318.2	0.42	ribosomal	canucin A, canucin B	Streptomyces canus
BGC0001552.3	0.41	ribosomal	citrulassin F	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001550.3	0.40	ribosomal	citrulassin D	Streptomyces katrae
BGC0002825.2	0.40	ribosomal	nocapeptin A	Nocardia terpenica IFM 0406
BGC0001551.4	0.39	ribosomal	citrulassin E	Streptomyces glaucescens
BGC0001889.2	0.39	ribosomal	fusilassin	Thermobifida fusca YX
BGC0000285.5	0.37	PKS, saccharide	flaviolin, flaviolin rhamnoside, 3,3'-diflaviolin	Saccharopolyspora erythraea

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lasso peptide predictions

Lassopeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.39 out of 19.26

Confidence: strong

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 22.87 out of 26.33

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 18.08 out of 25.31

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

Additional weak hits (5):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.15 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.92 out of 27.78

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.35 out of 27.55

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.65 out of 32.93

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.48 out of 20.01

Confidence: weak

Lasso peptides

TFBS Finder

Core peptide predictions

No core peptides found.

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
AfsR	Pleiotropic regulatory for antibiotic production
CRP	Cyclic AMP receptor protein
CatR	H2O2-responsive repressor

NZ_JALMDK010000004.1 - Region 2 - betalactone,hydrogen-cyanide

Download region GenBank file

Download region SVG

Location: 192,137 - 227,445 nt. (total: 35,309 nt) Show pHMM detection rules used

hydrogen-cyanide: (Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)
betalactone: (HMGL-like and AMP-binding)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase reductase

MZC64_RS16705: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

MZC64_RS16720: alkyl hydroperoxide reductase subunit

smcogs: SMCOG1176: alkyl hydroperoxide reductase subunit

MZC64_RS16730: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

MZC64_RS16765: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

MZC64_RS16770: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

MZC64_RS16785: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

MZC64_RS16790: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

MZC64_RS16795: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

MZC64_RS16800: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

MZC64_RS16815: acyl-CoA dehydrogenase type 2

smcogs: SMCOG1191: acyl-CoA dehydrogenase type 2

Transferases: acetyltransferase

MZC64_RS16755: acetyl-CoA acetyltransferase

smcogs: SMCOG1124: acetyl-CoA acetyltransferase

Hydrolases: dehalogenase peptidase

MZC64_RS16700: methionine aminopeptidase

smcogs: SMCOG1172: methionine aminopeptidase

MZC64_RS16810: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

Lyases: hydratase

MZC64_RS16780: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: ligase synthase

MZC64_RS16775: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

MZC64_RS16820: 2-isopropylmalate synthase

smcogs: SMCOG1271: 2-isopropylmalate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000866.5	0.36	other (other)	polyhydroxyalkanoate	Burkholderia sp. DSM 9242
BGC0000867.3	0.34	other (other)	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0002276.2	0.24	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0002274.2	0.23	NRPS	aspulvinone H, aspulvinone B1	Aspergillus terreus NIH2624
BGC0002345.2	0.21	other (other)	hydrogen cyanide	Pseudomonas fluorescens
BGC0001945.3	0.19	NRPS	chloromyxamide	Myxococcus sp.
BGC0000274.5	0.18	PKS	tetarimycin A, tetarimycin B	uncultured bacterium
BGC0002556.2	0.18	other (other)	cervinomycin B3, cervinomycin C1, cervinomycin C2, cervinomycin C3, cervinomycin C4	Streptomyces sp.
BGC0000131.5	0.18	PKS	pyrrolomycin	Streptomyces sp. UC 11065
BGC0000883.5	0.18	other (other)	arginomycin	Streptomyces arginensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002276.2	0.57	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0002274.2	0.56	NRPS	aspulvinone H, aspulvinone B1	Aspergillus terreus NIH2624
BGC0002345.2	0.53	other (other)	hydrogen cyanide	Pseudomonas fluorescens
BGC0000866.5	0.53	other (other)	polyhydroxyalkanoate	Burkholderia sp. DSM 9242
BGC0000867.3	0.51	other (other)	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0000131.5	0.50	PKS	pyrrolomycin	Streptomyces sp. UC 11065
BGC0001464.3	0.47	other (other)	tetrabromopyrrole	Pseudoalteromonas sp. PS5
BGC0001928.2	0.46	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0000888.5	0.46	other (other)	bacilysin	Bacillus sp. CS93
BGC0000207.5	0.45	PKS	chartreusin	Streptomyces chartreusis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 21.43 out of 27.04

Confidence: medium

Additional weak hits (6):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.65 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.33 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.19 out of 18.57

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.14 out of 36.54

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.83 out of 21.55

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 21.69 out of 36.53

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
DasR	N-acetylglucosamine dependent repressor

NZ_JALMDK010000004.1 - Region 3 - indole

Download region GenBank file

Download region SVG

Location: 289,053 - 310,150 nt. (total: 21,098 nt) Show pHMM detection rules used

indole: (indsynth or dmat or indole_PTase)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

MZC64_RS17145: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001294.5	0.54	other (other)	7-prenylisatin	Streptomyces sp. MBT28
BGC0002128.3	0.43	other (other)	5-dimethylallylindole-3-acetonitrile	Streptomyces coelicolor A3(2)
BGC0001483.4	0.38	other (other)	5-isoprenylindole-3-carboxylate β-D-glycosyl ester	Streptomyces sp. RM-5-8
BGC0002535.2	0.35	NRPS	γ-poly-L-2,4-diaminobutyric acid	Streptomyces celluloflavus
BGC0001197.5	0.33	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0002643.2	0.33	other (other)	aetokthonotoxin	Aetokthonos hydrillicola Thurmond2011
BGC0000878.5	0.32	other (other)	puromycin	Streptomyces alboniger
BGC0001911.3	0.32	terpene	raimonol	Streptomyces anulatus
BGC0001910.4	0.31	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0002253.3	0.31	PKS	waikikiamide A, waikikiamide B, waikikiamide C, (+)-semivioxanthin, semivioxanthin	Aspergillus sp. FM242

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001294.5	0.54	other (other)	7-prenylisatin	Streptomyces sp. MBT28
BGC0002128.3	0.43	other (other)	5-dimethylallylindole-3-acetonitrile	Streptomyces coelicolor A3(2)
BGC0001483.4	0.38	other (other)	5-isoprenylindole-3-carboxylate β-D-glycosyl ester	Streptomyces sp. RM-5-8
BGC0002535.2	0.35	NRPS	γ-poly-L-2,4-diaminobutyric acid	Streptomyces celluloflavus
BGC0001197.5	0.33	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0002643.2	0.33	other (other)	aetokthonotoxin	Aetokthonos hydrillicola Thurmond2011
BGC0000878.5	0.32	other (other)	puromycin	Streptomyces alboniger
BGC0001911.3	0.32	terpene	raimonol	Streptomyces anulatus
BGC0001910.4	0.31	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0002253.3	0.31	PKS	waikikiamide A, waikikiamide B, waikikiamide C, (+)-semivioxanthin, semivioxanthin	Aspergillus sp. FM242

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 24.54 out of 30.22

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.38 out of 19.26

Confidence: medium

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.07 out of 26.85

Confidence: medium

Additional weak hits (7):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.38 out of 21.84

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.20 out of 25.88

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.85 out of 30.97

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.79 out of 30.25

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.01 out of 28.19

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.20 out of 30.11

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 23.07 out of 30.11

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
ClgR	Caseinolytic protease gene regulator
RutR	Regulator of the pyrimidine and purine metabolism

NZ_JALMDK010000004.1 - Region 4 - RRE-containing

Download region GenBank file

Download region SVG

Location: 451,469 - 472,575 nt. (total: 21,107 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002317.2	0.49	ribosomal	herbicolin O, mcceh252, pantocin A	Pantoea agglomerans
BGC0000908.3	0.40	other (other)	melanin	Streptomyces avermitilis
BGC0000528.5	0.40	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000585.5	0.40	ribosomal	microcin C7	Escherichia coli
BGC0000503.5	0.37	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002316.2	0.36	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0000043.5	0.36	PKS	curacin A	Moorea producens 19L
BGC0002346.3	0.36	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0002307.3	0.32	ribosomal	humidimycin	Streptomyces humidus
BGC0002064.2	0.30	ribosomal	felipeptin A2, felipeptin A1	Amycolatopsis sp. YIM 10

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002317.2	0.49	ribosomal	herbicolin O, mcceh252, pantocin A	Pantoea agglomerans
BGC0000908.3	0.40	other (other)	melanin	Streptomyces avermitilis
BGC0000528.5	0.40	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000585.5	0.40	ribosomal	microcin C7	Escherichia coli
BGC0000503.5	0.37	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002316.2	0.36	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0000043.5	0.36	PKS	curacin A	Moorea producens 19L
BGC0002346.3	0.36	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0002307.3	0.32	ribosomal	humidimycin	Streptomyces humidus
BGC0002064.2	0.30	ribosomal	felipeptin A2, felipeptin A1	Amycolatopsis sp. YIM 10

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.46 out of 20.47

Confidence: strong

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 18.25 out of 20.01

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (7):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.76 out of 29.38

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.87 out of 21.55

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.41 out of 32.74

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 21.17 out of 27.55

Confidence: weak

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 23.04 out of 29.51

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.13 out of 25.75

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.18 out of 31.73

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: Pantocin_Microcin_RRE; Location: 0 - 92; Score: 49.2

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
ArgR	Regulator of arginine biosynthesis genes
VqsM	Regulator of quorum-sensing signalling systems

NZ_JALMDK010000004.1 - Region 5 - NI-siderophore

Download region GenBank file

Download region SVG

Location: 594,774 - 626,399 nt. (total: 31,626 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

MZC64_RS18620: lysine/ornithine N-monooxygenase

smcogs: SMCOG1080: lysine/ornithine N-monooxygenase

Transferases: aminotransferase

MZC64_RS18630: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Hydrolases: protease

MZC64_RS18510: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: decarboxylase

MZC64_RS18585: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

MZC64_RS18625: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

Ligases: synthase

MZC64_RS18550: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001478.5	0.65	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0001535.3	0.64	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0000941.3	0.62	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001572.3	0.61	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0001531.5	0.60	other (other)	bisucaberin B	Tenacibaculum mesophilum
BGC0002305.2	0.59	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0001534.3	0.57	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0002469.2	0.57	other (other)	woodybactin A, woodybactin B, woodybactin C, woodybactin D	Shewanella woodyi ATCC 51908
BGC0002527.2	0.55	other (other)	vibrioferrin	Azotobacter vinelandii CA
BGC0001453.5	0.53	other (other)	desferrioxamine	Streptomyces argillaceus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001478.5	0.65	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0001535.3	0.64	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0000941.3	0.62	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001572.3	0.61	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0001531.5	0.60	other (other)	bisucaberin B	Tenacibaculum mesophilum
BGC0002305.2	0.59	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0001534.3	0.57	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0002469.2	0.57	other (other)	woodybactin A, woodybactin B, woodybactin C, woodybactin D	Shewanella woodyi ATCC 51908
BGC0002527.2	0.55	other (other)	vibrioferrin	Azotobacter vinelandii CA
BGC0001453.5	0.53	other (other)	desferrioxamine	Streptomyces argillaceus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.24 out of 19.26

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.52 out of 23.79

Confidence: medium

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 23.43 out of 26.85

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.09 out of 23.79

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 22.15 out of 24.78

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.74 out of 20.47

Confidence: medium

Additional weak hits (9):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.06 out of 26.33

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.62 out of 26.06

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.92 out of 26.33

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.26 out of 27.78

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.42 out of 21.55

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.37 out of 36.54

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.58 out of 27.78

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.91 out of 24.78

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.33 out of 29.65

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CRP	Cyclic AMP receptor protein
IolR	Regulator of the inositol catabolism
LexA variant 2	Repressor of DNA damage response
RutR	Regulator of the pyrimidine and purine metabolism
VqsM	Regulator of quorum-sensing signalling systems

NZ_JALMDK010000004.1 - Region 6 - terpene

Download region GenBank file

Download region SVG

Location: 642,012 - 663,055 nt. (total: 21,044 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

MZC64_RS18725: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

MZC64_RS18730: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: glycosyltransferase

MZC64_RS18755: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

MZC64_RS48725: glycosyltransferase

smcogs: SMCOG1102: glycosyltransferase

Hydrolases: protease

MZC64_RS18770: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: cyclase

MZC64_RS18760: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002631.2	0.54	ribosomal	Myxarylin	Pyxidicoccus fallax
BGC0000844.5	0.48	other (other)	clavulanic acid	Streptomyces clavuligerus
BGC0002103.2	0.45	other (other)	pulcherriminic acid	Bacillus subtilis subsp. subtilis str. 168
BGC0001336.5	0.43	other (other)	erdasporine A, erdasporine B, erdasporine C	uncultured bacterium
BGC0000597.4	0.42	ribosomal	SWA-2138	Streptomyces sp. e14
BGC0001483.4	0.41	other (other)	5-isoprenylindole-3-carboxylate β-D-glycosyl ester	Streptomyces sp. RM-5-8
BGC0000665.5	0.41	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0002482.2	0.40	other (other)	tjipanazole D, tjipanazole I, tjipanazole L, tjipanazole K, tjipanazole M	Symphyonema bifilamentata 97.28
BGC0001605.3	0.40	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0000595.3	0.39	ribosomal	SCO-2138	Streptomyces coelicolor A3(2)

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002631.2	0.54	ribosomal	Myxarylin	Pyxidicoccus fallax
BGC0000844.5	0.48	other (other)	clavulanic acid	Streptomyces clavuligerus
BGC0002103.2	0.45	other (other)	pulcherriminic acid	Bacillus subtilis subsp. subtilis str. 168
BGC0001336.5	0.43	other (other)	erdasporine A, erdasporine B, erdasporine C	uncultured bacterium
BGC0000597.4	0.42	ribosomal	SWA-2138	Streptomyces sp. e14
BGC0001483.4	0.41	other (other)	5-isoprenylindole-3-carboxylate β-D-glycosyl ester	Streptomyces sp. RM-5-8
BGC0000665.5	0.41	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0002482.2	0.40	other (other)	tjipanazole D, tjipanazole I, tjipanazole L, tjipanazole K, tjipanazole M	Symphyonema bifilamentata 97.28
BGC0001605.3	0.40	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0000595.3	0.39	ribosomal	SCO-2138	Streptomyces coelicolor A3(2)

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

No product predictions

Genes with terpene domains:

MZC64_RS18760: T1TS

Domain 1 (12 - 313): Type 1 terpene synthase

Subtype: unknown

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.68 out of 19.52

Confidence: medium

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 20.01 out of 23.43

Confidence: medium

Additional weak hits (8):

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.32 out of 31.73

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.82 out of 30.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.54 out of 27.78

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.88 out of 29.38

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.55 out of 30.25

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 22.43 out of 29.65

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.50 out of 26.06

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.36 out of 28.19

Confidence: weak

Terpene

TFBS Finder

Glossary

No acronyms to display

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
BldD	Development and antibiotic global regulator

NZ_JALMDK010000004.1 - Region 7 - furan

Download region GenBank file

Download region SVG

Location: 814,281 - 835,279 nt. (total: 20,999 nt) Show pHMM detection rules used

furan: (mmyO or AvrD)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: synthase

MZC64_RS19380: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000914.5	0.39	other (other)	methylenomycin A	Streptomyces coelicolor A3(2)
BGC0002554.2	0.38	other (other)	carquinostatin A, carquinostatin B	Streptomyces exfoliatus
BGC0001903.3	0.36	other (other)	neocarazostatin A	Streptomyces sp. MA37
BGC0001364.3	0.35	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919
BGC0002711.2	0.34	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0001810.3	0.33	PKS	trehangelin	Polymorphospora rubra
BGC0000286.3	0.33	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0001366.5	0.33	PKS	BE-24566B, zunyimycin A	Streptomyces sp. FJS31-2
BGC0002037.3	0.33	NRPS	azetidomonamide A, azetidomonamide B	Pseudomonas aeruginosa PAO1
BGC0001080.5	0.33	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000914.5	0.39	other (other)	methylenomycin A	Streptomyces coelicolor A3(2)
BGC0002554.2	0.38	other (other)	carquinostatin A, carquinostatin B	Streptomyces exfoliatus
BGC0001903.3	0.36	other (other)	neocarazostatin A	Streptomyces sp. MA37
BGC0001364.3	0.35	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919
BGC0002711.2	0.34	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0001810.3	0.33	PKS	trehangelin	Polymorphospora rubra
BGC0000286.3	0.33	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0001366.5	0.33	PKS	BE-24566B, zunyimycin A	Streptomyces sp. FJS31-2
BGC0002037.3	0.33	NRPS	azetidomonamide A, azetidomonamide B	Pseudomonas aeruginosa PAO1
BGC0001080.5	0.33	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.00 out of 20.47

Confidence: strong

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 22.96 out of 29.72

Confidence: medium

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.73 out of 18.46

Confidence: medium

Additional weak hits (2):

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.33 out of 25.75

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.57 out of 24.78

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
NtrC	Nitrogen regulatory protein C
RpoN	Regulator of nitrogen assimilation and virulence
VqsM	Regulator of quorum-sensing signalling systems

NZ_JALMDK010000005.1 - Region 1 - terpene-precursor

Download region GenBank file

Download region SVG

Location: 58,193 - 79,284 nt. (total: 21,092 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: synthetase

MZC64_RS20095: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000563.4	0.48	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0001226.4	0.48	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0001579.4	0.41	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0000616.4	0.40	ribosomal	amylocyclicin	Bacillus velezensis FZB42
BGC0000528.5	0.40	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000503.5	0.39	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0001684.3	0.38	other (other)	N-acyloxyacyl glutamine	Klebsiella variicola
BGC0001660.3	0.38	ribosomal	mathermycin	Marinactinospora thermotolerans
BGC0002346.3	0.38	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0002309.2	0.38	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000563.4	0.48	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0001226.4	0.48	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0001579.4	0.41	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0000616.4	0.40	ribosomal	amylocyclicin	Bacillus velezensis FZB42
BGC0000528.5	0.40	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000503.5	0.39	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0001684.3	0.38	other (other)	N-acyloxyacyl glutamine	Klebsiella variicola
BGC0001660.3	0.38	ribosomal	mathermycin	Marinactinospora thermotolerans
BGC0002346.3	0.38	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0002309.2	0.38	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

MZC64_RS20095: PT_FPPS_like

Domain 1 (46 - 355): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.21 out of 20.47

Confidence: medium

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 27.38 out of 29.65

Confidence: strong

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 23.11 out of 24.78

Confidence: strong

Additional weak hits (4):

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 21.38 out of 36.54

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 20.09 out of 36.54

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.12 out of 27.78

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.39 out of 24.78

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
LexA variant 1	Repressor of DNA damage response
LexA variant 2	Repressor of DNA damage response
VqsM	Regulator of quorum-sensing signalling systems

NZ_JALMDK010000005.1 - Region 2 - RiPP-like

Download region GenBank file

Download region SVG

Location: 396,490 - 407,305 nt. (total: 10,816 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	RiPP-like	Similarity score	Type	Compound(s)	Organism
BGC0000904.5		0.24	other (other)	FR-900098	Streptomyces rubellomurinus

Reference	Aggregated	Similarity score	Type	Compound(s)	Organism
BGC0000904.5		0.24	other (other)	FR-900098	Streptomyces rubellomurinus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.22 out of 26.85

Confidence: medium

Additional weak hits (2):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.80 out of 27.78

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 21.66 out of 27.18

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
RutR	Regulator of the pyrimidine and purine metabolism

NZ_JALMDK010000005.1 - Region 3 - NRPS,T1PKS

Download region GenBank file

Download region SVG

Location: 488,960 - 645,687 nt. (total: 156,728 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase reductase

MZC64_RS22080: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

MZC64_RS22120: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

MZC64_RS22190: 3-hydroxyisobutyrate dehydrogenase

smcogs: SMCOG1100: 3-hydroxyisobutyrate dehydrogenase

MZC64_RS22290: alkyl hydroperoxide reductase subunit

smcogs: SMCOG1176: alkyl hydroperoxide reductase subunit

Transferases: PPTase acylase acyltransferase aminotransferase glycosyltransferase methyltransferase

MZC64_RS22050: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1060: 4'-phosphopantetheinyl transferase

MZC64_RS22110: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

MZC64_RS22125: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

MZC64_RS22130: methyltransferase

smcogs: SMCOG1248: methyltransferase

MZC64_RS22145: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

MZC64_RS22215: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

MZC64_RS22265: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1060: 4'-phosphopantetheinyl transferase

MZC64_RS47940: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase hydrolase

MZC64_RS22115: thioesterase

smcogs: SMCOG1004: thioesterase

MZC64_RS22210: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

MZC64_RS22280: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Ligases: ligase synthase

MZC64_RS22060: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

MZC64_RS22065: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

MZC64_RS22100: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

MZC64_RS22105: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

MZC64_RS22220: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000898.5	0.49	saccharide	desosamine, pikromycin, neomethymycin, narbomycin	Streptomyces venezuelae
BGC0002135.2	0.44	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001273.3	0.44	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.43	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.43	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.43	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001135.5	0.42	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000041.3	0.41	PKS	oronofacic acid	Pseudomonas syringae
BGC0001261.3	0.41	NRPS	AM-toxin	Alternaria alternata
BGC0001128.4	0.40	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001273.3	0.74	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.74	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.74	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.74	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000041.3	0.73	PKS	oronofacic acid	Pseudomonas syringae
BGC0001261.3	0.73	NRPS	AM-toxin	Alternaria alternata
BGC0002286.3	0.72	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.00 out of 20.47

Confidence: strong

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.56 out of 24.78

Confidence: medium

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.47 out of 26.85

Confidence: medium

Additional weak hits (19):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.70 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.86 out of 29.38

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.81 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.48 out of 27.78

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.77 out of 28.14

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.98 out of 27.55

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.40 out of 26.33

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.40 out of 21.84

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.07 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.84 out of 27.78

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.47 out of 21.55

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.30 out of 21.55

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.38 out of 21.84

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 23.20 out of 30.22

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.16 out of 26.06

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.31 out of 28.19

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 24.02 out of 36.53

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.69 out of 24.78

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.09 out of 23.43

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 3, location 488959 - 645687:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - ccmal) + (ohmal - ohmal - ccmal) + (Me-ohmal - Me-redmal) + (Me-ohmal) + (ccmal) + (Me-ohmal - Me-ccmal) + (Me-mal) + (Leu - D-Ala - D-Val - D-Val - Leu) + (D-Val - Orn - D-Val - Orn - D-X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 488959 - 585833:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - ccmal) + (ohmal - ohmal - ccmal) + (Me-ohmal - Me-redmal) + (Me-ohmal) + (ccmal) + (Me-ohmal - Me-ccmal) + (Me-mal)
Non-colinear ordering:: MZC64_RS22060 + MZC64_RS22065 + MZC64_RS22080 + MZC64_RS47940 + MZC64_RS22100 + MZC64_RS22105 + MZC64_RS22110

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 5, location 567958 - 645687:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Leu - D-Ala - D-Val - D-Val - Leu) + (D-Val - Orn - D-Val - Orn - D-X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MZC64_RS22060: mal - mal

PKS_AT (534..804): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 220.2
inactive: 114.3
Methoxymalonyl-CoA: 63.1
Methylmalonyl-CoA: 57.1
Propionyl-CoA: 35.6
Isobutyryl-CoA: 26.9
Acetyl-CoA: 26.2
Benzoyl-CoA: 21.1
Ethylmalonyl-CoA: 20.1
CHC-CoA: 18.2
fatty_acid: 9.3
2-Methylbutyryl-CoA: 7.6
3-Methylbutyryl-CoA: 0.0
trans-1,2-CPDA: -1.6

PKS_AT (1473..1758): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.0
inactive: 114.2
Methoxymalonyl-CoA: 69.8
Methylmalonyl-CoA: 59.1
Propionyl-CoA: 37.9
Isobutyryl-CoA: 31.4
Acetyl-CoA: 29.8
Benzoyl-CoA: 21.5
Ethylmalonyl-CoA: 21.1
CHC-CoA: 18.9
2-Methylbutyryl-CoA: 15.5
trans-1,2-CPDA: 13.5
fatty_acid: 12.6
3-Methylbutyryl-CoA: 0.0

MZC64_RS22065: mal - mal - mal

PKS_AT (573..861): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 229.3
inactive: 123.2
Methoxymalonyl-CoA: 73.5
Methylmalonyl-CoA: 71.3
Isobutyryl-CoA: 47.2
Propionyl-CoA: 41.6
Benzoyl-CoA: 30.8
Ethylmalonyl-CoA: 28.3
Acetyl-CoA: 27.6
2-Methylbutyryl-CoA: 21.5
CHC-CoA: 18.2
trans-1,2-CPDA: 11.8
fatty_acid: 10.4
3-Methylbutyryl-CoA: 8.6

PKS_AT (1991..2274): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 217.9
inactive: 119.9
Methoxymalonyl-CoA: 68.7
Methylmalonyl-CoA: 63.0
Propionyl-CoA: 50.4
Isobutyryl-CoA: 30.4
Ethylmalonyl-CoA: 28.6
2-Methylbutyryl-CoA: 27.9
Acetyl-CoA: 25.8
Benzoyl-CoA: 24.6
CHC-CoA: 19.5
trans-1,2-CPDA: 13.9
fatty_acid: 10.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (3457..3749): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 234.3
inactive: 127.3
Methoxymalonyl-CoA: 74.6
Methylmalonyl-CoA: 71.4
Ethylmalonyl-CoA: 42.8
Propionyl-CoA: 40.2
Isobutyryl-CoA: 36.2
2-Methylbutyryl-CoA: 27.8
Acetyl-CoA: 26.3
Benzoyl-CoA: 23.9
trans-1,2-CPDA: 22.9
fatty_acid: 8.4
CHC-CoA: 7.5
3-Methylbutyryl-CoA: 6.4

MZC64_RS22080: mmal - mmal

PKS_AT (553..849): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 221.6
Ethylmalonyl-CoA: 216.1
Methoxymalonyl-CoA: 183.3
2-Methylbutyryl-CoA: 159.4
trans-1,2-CPDA: 125.7
Propionyl-CoA: 112.6
CHC-CoA: 94.1
Malonyl-CoA: 92.1
Isobutyryl-CoA: 85.0
Benzoyl-CoA: 68.4
Acetyl-CoA: 55.0
3-Methylbutyryl-CoA: 49.7
inactive: 42.0
fatty_acid: 22.6

PKS_AT (2049..2340): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 214.4
Ethylmalonyl-CoA: 195.5
Methoxymalonyl-CoA: 175.7
2-Methylbutyryl-CoA: 145.1
trans-1,2-CPDA: 129.7
Propionyl-CoA: 109.4
Malonyl-CoA: 95.7
CHC-CoA: 95.7
Isobutyryl-CoA: 77.1
Benzoyl-CoA: 51.4
Acetyl-CoA: 43.6
3-Methylbutyryl-CoA: 39.3
inactive: 34.6
fatty_acid: 22.2

MZC64_RS47940: mmal

PKS_AT (563..855): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 227.1
Ethylmalonyl-CoA: 217.6
Methoxymalonyl-CoA: 186.7
2-Methylbutyryl-CoA: 161.4
trans-1,2-CPDA: 134.1
Propionyl-CoA: 111.1
Malonyl-CoA: 96.9
Isobutyryl-CoA: 93.6
CHC-CoA: 91.5
Benzoyl-CoA: 81.4
Acetyl-CoA: 53.6
3-Methylbutyryl-CoA: 42.0
fatty_acid: 29.3
inactive: 26.4

MZC64_RS22100: mal

PKS_AT (556..834): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 221.0
inactive: 112.8
Methoxymalonyl-CoA: 67.7
Methylmalonyl-CoA: 61.1
Propionyl-CoA: 49.5
Isobutyryl-CoA: 33.5
Acetyl-CoA: 28.7
2-Methylbutyryl-CoA: 19.6
Ethylmalonyl-CoA: 18.7
CHC-CoA: 17.3
Benzoyl-CoA: 14.1
trans-1,2-CPDA: 11.2
fatty_acid: 7.7
3-Methylbutyryl-CoA: 0.0

MZC64_RS22105: mmal - mmal

PKS_AT (549..836): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 221.7
Ethylmalonyl-CoA: 213.3
Methoxymalonyl-CoA: 187.6
2-Methylbutyryl-CoA: 158.1
trans-1,2-CPDA: 126.1
Propionyl-CoA: 115.1
CHC-CoA: 103.2
Malonyl-CoA: 96.6
Isobutyryl-CoA: 81.8
Benzoyl-CoA: 68.8
Acetyl-CoA: 54.6
3-Methylbutyryl-CoA: 46.2
inactive: 39.6
fatty_acid: 27.9

PKS_AT (2050..2344): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 215.3
Ethylmalonyl-CoA: 208.0
Methoxymalonyl-CoA: 175.9
2-Methylbutyryl-CoA: 158.0
trans-1,2-CPDA: 133.5
Propionyl-CoA: 115.1
CHC-CoA: 99.5
Malonyl-CoA: 98.8
Isobutyryl-CoA: 97.7
Benzoyl-CoA: 71.9
3-Methylbutyryl-CoA: 55.5
Acetyl-CoA: 53.6
inactive: 46.7
fatty_acid: 26.7

MZC64_RS22110: mmal

PKS_AT (566..859): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 223.0
Ethylmalonyl-CoA: 210.0
Methoxymalonyl-CoA: 184.8
trans-1,2-CPDA: 152.1
2-Methylbutyryl-CoA: 150.4
Propionyl-CoA: 115.4
Malonyl-CoA: 102.7
CHC-CoA: 94.3
Isobutyryl-CoA: 77.3
Benzoyl-CoA: 69.5
Acetyl-CoA: 47.8
3-Methylbutyryl-CoA: 44.6
inactive: 34.2
fatty_acid: 22.5

MZC64_RS22215: Leu - Ala - Val - Val - Leu

Search NORINE for peptide: strict or relaxed

AMP-binding (51..429): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAESFDASVLEMLIIVGGDINSYGPTESTVVATW
Nearest Stachelhaus code(s):: Leu or 3OH-Leu DALLIGAVVK (85% 8Å match); Leu DALLIGAVVK (65% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (1074..1459): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: LWATFDVSVFECSAVLAGEANIYGPTEATVYATA
Nearest Stachelhaus code(s):: Ala DVFSVAIVYK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2532..2925): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LHRSFDAVTYEMFLWTGGEVDVYGPTETTTFATH
Nearest Stachelhaus code(s):: Val DAYFWGVTFK (88% 8Å match); Leu DAYFWGVTFK (85% 8Å match); Ile DAYFWGVTFK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4009..4414): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LDRSFDAATYEVWLWTGGDVDGYGPTETTTFATH
Nearest Stachelhaus code(s):: Val DAYWWGGTFK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5484..5861): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAESFDASVLEMLIIVGGDINSYGPTESTVVTTW
Nearest Stachelhaus code(s):: Leu or 3OH-Leu DALLIGAVVK (82% 8Å match); Leu DALLIGAVVK (62% 8Å match)
Stachelhaus code match:: 90% (moderate)

MZC64_RS22220: Val - Orn - Val - Orn - X

Search NORINE for peptide: strict or relaxed

AMP-binding (493..887): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LEHSFDASTYEVFLWTGGDSDVYGPTETTTYATQ
Nearest Stachelhaus code(s):: Ile DAYFWGVTFK (82% 8Å match); Val DAYFWGVTFK (82% 8Å match); Leu DAYFWGVTFK (79% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (1995..2377): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LNHAFDTSWEGGDLMLGGEYNYYGPTECVVDAVY
Nearest Stachelhaus code(s):: Orn DTEDMGYVDK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3013..3401): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LDRSFDASTYEVWLWTGGDVDGYGPTETTTFAST
Nearest Stachelhaus code(s):: Val DAYWWGGTFK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4487..4870): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LNHAFDTSLEGLDLMLGGEYNFYGPTECTVDALS
Nearest Stachelhaus code(s):: Orn DTEDMGFVDK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5514..5889): X

nrpys: Leu, 3OH-Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAEAFDASVLEMLIIVGGDINAYGPTESTVVATW
Nearest Stachelhaus code(s):: Leu or 3OH-Leu DALLIGAVVK (85% 8Å match); Leu DALLIGAVVK (65% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
LexA variant 2	Repressor of DNA damage response
RutR	Regulator of the pyrimidine and purine metabolism
VqsM	Regulator of quorum-sensing signalling systems

NZ_JALMDK010000006.1 - Region 1 - PKS-like,T1PKS,T2PKS,lanthipeptide-class-iii

Download region GenBank file

Download region SVG

Location: 600,793 - 698,907 nt. (total: 98,115 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
PKS-like: (t2pks2 or ksIII)
T2PKS: (t2ks and t2clf)
lanthipeptide-class-iii: (micKC and not PKS_KS)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

MZC64_RS25995: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

MZC64_RS26070: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

MZC64_RS26080: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

MZC64_RS26155: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acetyltransferase acylase aminotransferase glycosyltransferase methyltransferase transferase

MZC64_RS26115: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

MZC64_RS26120: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

MZC64_RS26190: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

MZC64_RS26200: methyltransferase

smcogs: SMCOG1248: methyltransferase

MZC64_RS26210: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

MZC64_RS26215: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

MZC64_RS26225: aminotransferase

smcogs: SMCOG1019: aminotransferase

MZC64_RS26305: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

MZC64_RS26335: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: hydrolase phosphatase

MZC64_RS26055: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

MZC64_RS26265: inositol monophosphatase

smcogs: SMCOG1207: inositol monophosphatase

Lyases: cyclase dehydratase

MZC64_RS26060: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

MZC64_RS26110: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

MZC64_RS26185: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

MZC64_RS26205: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

MZC64_RS26000: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

MZC64_RS26075: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

MZC64_RS26170: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

MZC64_RS26175: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

MZC64_RS26295: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

MZC64_RS26340: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	1.15	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	1.14	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	1.14	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	1.13	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002191.2	0.89	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.85	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001254.3	0.84	PKS	ACT-Toxin II	Alternaria alternata
BGC0002192.3	0.84	PKS	FR901512	fungal sp. No.14919
BGC0000107.3	0.80	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0000037.4	0.78	PKS	cichorine	Aspergillus nidulans FGSC A4

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000231.5	0.64	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001164.3	0.64	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001273.3	0.64	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.64	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.64	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.64	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001254.3	0.62	PKS	ACT-Toxin II	Alternaria alternata

Similar known clusters from MIBiG 4.0

Similar subclusters

Lanthipeptide predictions

Lanthipeptide(s) for MZC64_RS26310

MZC64_RS26315

Leader:

MNTMAILDLQGLDTNAAE

Core:

LTMTGSTVSNGC

Core with dehydrations:

LDhbMDhbGDhaDhbVDhaNGCys

antiSMASH-DB 4.0 matches for MZC64_RS26315

100.0%

NZ_JALMDK010000006.1:

Lanthipeptides: MZC64_RS26315_lanthipeptide

1

LTMTGSTVSNGC

12

LTMTGSTVSNGC

1

LTMTGSTVSNGC

12

91.7%

NZ_JALMDK010000006.1:

Lanthipeptides: MZC64_RS26320_lanthipeptide

1

LTMTGSTVSNGC

12

LTMTGSTVSN C

1

LTMTGSTVSNNC

12

58.3%

NZ_BMVC01000018.1and 2 others:

NZ_BMVC01000018.1 Lanthipeptides: IE282_RS34450_lanthipeptideNZ_BMVC01000018.1 Lanthipeptides: IE282_RS34445_lanthipeptide

Lanthipeptides: IE282_RS34455_lanthipeptide

5

GSTVSNGC

12

GSTVSN C

2

GSTVSNNC

9

with 8 more sequence matches

58.3%

NZ_JANBBG010000004.1and 1 other:

NZ_JANBBG010000004.1 Lanthipeptides: allorf_1567737_1567829_lanthipeptide

Lanthipeptides: allorf_1567890_1567982_lanthipeptide

3

MTGSTVSNGC

12

+ GSTVSN C

1

LPGSTVSNNC

10

50.0%

NZ_JANAVP010000029.1:

Lanthipeptides: NLX86_RS27125_lanthipeptide

7

TVSNGC

12

TVSNGC

1

TVSNGC

6

50.0%

NZ_KB900388.1:

Lanthipeptides: allorf_4143261_4143347_lanthipeptide

1

LTMTGSTVSNGC

12

+ STVSN C

1

IAAPASTVSNSC

12

40.0%

NZ_CP068437.1and 2 others:

NZ_CP068437.1 Lanthipeptides: JK628_RS03400_lanthipeptideNZ_CP068437.1 Lanthipeptides: JK628_RS03395_lanthipeptide

Lanthipeptides: allorf_0778008_0778106_lanthipeptide

3

MTGSTVSNGC

12

M ST+SN C

2

MEWSTISNHC

11

31.8%

NZ_LN868937.1:

Lanthipeptides: BN2144_RS19915_lanthipeptide

1

LTMTGSTVSNGC

12

L +T ST+SN C

11

LLVTTSTISNHC

22

30.0%

NZ_JAFFJY010000011.1:

Lanthipeptides: JVX89_RS12320_lanthipeptide

6

STVSNGC

12

ST+SNGC

14

STISNGC

20

27.6%

NZ_FOHE01000012.1:

Lanthipeptides: BMX40_RS20340_lanthipeptide

1

LTMTGSTVSNGC

12

LT STVSN C

14

LTTFWSTVSNNC

25

27.3%

NZ_CP066711.1:

Lanthipeptides: JGZ56_RS07825_lanthipeptide

3

MTGSTVSNGC

12

+ GST+SN C

13

LGGSTISNHC

22

MIBiG 4.0 matches for MZC64_RS26315

21.7%

BGC0002111.3 (EVG22_32245)and 1 other:

BGC0002111.3 (multiple genes) Lanthipeptide: andalusicin A or andalusicin B

Lanthipeptide: andalusicin A or andalusicin B

5

GSTVSNGC

12

ST+SN C

16

ASTISNNC

23

MZC64_RS26320

Leader:

MNTMAILDLQGLDTNAAE

Core:

LTMTGSTVSNNC

Core with dehydrations:

LDhbMDhbGDhaDhbVDhaNNCys

antiSMASH-DB 4.0 matches for MZC64_RS26320

100.0%

NZ_JALMDK010000006.1:

Lanthipeptides: MZC64_RS26320_lanthipeptide

1

LTMTGSTVSNNC

12

LTMTGSTVSNNC

1

LTMTGSTVSNNC

12

91.7%

NZ_JALMDK010000006.1:

Lanthipeptides: MZC64_RS26315_lanthipeptide

1

LTMTGSTVSNNC

12

LTMTGSTVSN C

1

LTMTGSTVSNGC

12

66.7%

NZ_BMVC01000018.1and 2 others:

NZ_BMVC01000018.1 Lanthipeptides: IE282_RS34450_lanthipeptideNZ_BMVC01000018.1 Lanthipeptides: IE282_RS34445_lanthipeptide

Lanthipeptides: IE282_RS34455_lanthipeptide

5

GSTVSNNC

12

GSTVSNNC

2

GSTVSNNC

9

with 13 more sequence matches

Only the first 10 are displayed here.

66.7%

NZ_JANBBG010000004.1and 1 other:

NZ_JANBBG010000004.1 Lanthipeptides: allorf_1567737_1567829_lanthipeptide

Lanthipeptides: allorf_1567890_1567982_lanthipeptide

3

MTGSTVSNNC

12

+ GSTVSNNC

1

LPGSTVSNNC

10

50.0%

NZ_KB900388.1:

Lanthipeptides: allorf_4143261_4143347_lanthipeptide

1

LTMTGSTVSNNC

12

+ STVSN+C

1

IAAPASTVSNSC

12

40.0%

NZ_CP068437.1and 2 others:

NZ_CP068437.1 Lanthipeptides: JK628_RS03400_lanthipeptideNZ_CP068437.1 Lanthipeptides: JK628_RS03395_lanthipeptide

Lanthipeptides: allorf_0778008_0778106_lanthipeptide

3

MTGSTVSNNC

12

M ST+SN+C

2

MEWSTISNHC

11

38.9%

NZ_FOHE01000012.1and 1 other:

NZ_FOHE01000012.1 Lanthipeptides: BMX40_RS20540_lanthipeptide

Lanthipeptides: BMX40_RS20545_lanthipeptide

1

LTMTGSTVSNNC

12

+T+ STVSN+C

7

VTIFLSTVSNHC

18

35.3%

NZ_CP113837.1and 1 other:

NZ_CP113837.1 Lanthipeptides: OU787_RS28895_lanthipeptide

Lanthipeptides: OU787_RS28905_lanthipeptide

6

STVSNNC

12

STVSN+C

2

STVSNHC

8

31.8%

NZ_LN868937.1:

Lanthipeptides: BN2144_RS19915_lanthipeptide

1

LTMTGSTVSNNC

12

L +T ST+SN+C

11

LLVTTSTISNHC

22

31.6%

NC_014551.1and 54 others:

NC_014551.1 Lanthipeptides: BAMF_RS41665_lanthipeptideNC_014551.1 Lanthipeptides: BAMF_RS41670_lanthipeptideNC_014551.1 Lanthipeptides: BAMF_RS41655_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20950_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20940_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20945_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20930_lanthipeptideNZ_CP026010.1 Lanthipeptides: BS11774_RS22085_lanthipeptideNZ_CP026010.1 Lanthipeptides: BS11774_RS22075_lanthipeptideNZ_CP026010.1 Lanthipeptides: BS11774_RS22090_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20775_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20765_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20770_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20755_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21845_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21825_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21840_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21835_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04870_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04850_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04865_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04860_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03890_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03880_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03885_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03870_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19000_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19020_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19010_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19005_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20385_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20395_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20380_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21930_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21925_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21920_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21910_lanthipeptideNZ_JALAPV010000012.1 Lanthipeptides: MOC91_RS19790_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18885_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18865_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18880_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18875_lanthipeptideNZ_JALAUK010000033.1 Lanthipeptides: MOE38_RS18580_lanthipeptideNZ_JALAVZ010000001.1 Lanthipeptides: MOF00_RS00410_lanthipeptideNZ_JALAVZ010000001.1 Lanthipeptides: MOF00_RS00405_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19510_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19505_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19490_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19500_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19860_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19855_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19840_lanthipeptideNZ_LN868937.1 Lanthipeptides: BN2144_RS20095_lanthipeptideNZ_LN868937.1 Lanthipeptides: BN2144_RS20100_lanthipeptide

Lanthipeptides: BAMF_RS41675_lanthipeptide

6

STVSNNC

12

ST+SNNC

12

STISNNC

18

31.0%

NZ_FOHE01000012.1:

Lanthipeptides: BMX40_RS20340_lanthipeptide

1

LTMTGSTVSNNC

12

LT STVSNNC

14

LTTFWSTVSNNC

25

27.3%

NZ_CP018933.1and 24 others:

NZ_CP018933.1 Lanthipeptides: BEI49_RS29525_lanthipeptideNZ_CP018933.1 Lanthipeptides: BEI49_RS29520_lanthipeptideNZ_CP018933.1 Lanthipeptides: BEI49_RS29515_lanthipeptideNZ_CP018933.1 Lanthipeptides: BEI49_RS29510_lanthipeptideNZ_CP018933.1 Lanthipeptides: BEI49_RS29505_lanthipeptideNZ_CP018933.1 Lanthipeptides: BEI49_RS29500_lanthipeptideNZ_CP018933.1 Lanthipeptides: BEI49_RS29495_lanthipeptideNZ_CP018933.1 Lanthipeptides: BEI49_RS29490_lanthipeptideNZ_CP018933.1 Lanthipeptides: BEI49_RS29485_lanthipeptideNZ_CP018933.1 Lanthipeptides: BEI49_RS29480_lanthipeptideNZ_CP018933.1 Lanthipeptides: BEI49_RS29475_lanthipeptideNZ_CP085399.1 Lanthipeptides: LIS81_RS04325_lanthipeptideNZ_CP085399.1 Lanthipeptides: LIS81_RS04320_lanthipeptideNZ_CP085399.1 Lanthipeptides: LIS81_RS04315_lanthipeptideNZ_CP085399.1 Lanthipeptides: LIS81_RS04310_lanthipeptideNZ_CP085399.1 Lanthipeptides: LIS81_RS04305_lanthipeptideNZ_CP085399.1 Lanthipeptides: LIS81_RS04300_lanthipeptideNZ_CP085399.1 Lanthipeptides: LIS81_RS04295_lanthipeptideNZ_CP085399.1 Lanthipeptides: LIS81_RS04290_lanthipeptideNZ_CP085399.1 Lanthipeptides: LIS81_RS04285_lanthipeptideNZ_CP085399.1 Lanthipeptides: LIS81_RS04280_lanthipeptideNZ_NTVN01000027.1 Lanthipeptides: CN415_RS28750_lanthipeptideNZ_PJCU01000036.1 Lanthipeptides: CWE34_RS31080_lanthipeptideNZ_PJCU01000036.1 Lanthipeptides: CWE34_RS31075_lanthipeptide

Lanthipeptides: BEI49_RS29530_lanthipeptide

5

GSTVSNNC

12

STVSN+C

15

ASTVSNHC

22

27.3%

NZ_CP066711.1:

Lanthipeptides: JGZ56_RS07825_lanthipeptide

3

MTGSTVSNNC

12

+ GST+SN+C

13

LGGSTISNHC

22

MIBiG 4.0 matches for MZC64_RS26320

26.1%

BGC0002111.3 (EVG22_32245):

Lanthipeptide: andalusicin A or andalusicin B

5

GSTVSNNC

12

ST+SNNC

16

ASTISNNC

23

26.1%

BGC0002111.3 (multiple genes):

Lanthipeptide: andalusicin A or andalusicin B

3

MTGSTVSNNC

12

+ ST+SNNC

14

VWASTISNNC

23

allorf_688904_688996

Leader:

MTTMAILDLQGMETGAVE

Core:

LNMTGSSFSMHC

Core with dehydrations:

LNMDhbGDhaDhaFDhaMHCys

antiSMASH-DB 4.0 matches for allorf_688904_688996

100.0%

NZ_JALMDK010000006.1:

Lanthipeptides: allorf_688904_688996_lanthipeptide

1

LNMTGSSFSMHC

12

LNMTGSSFSMHC

1

LNMTGSSFSMHC

12

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.24 out of 26.85

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.38 out of 19.26

Confidence: medium

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 21.55 out of 27.04

Confidence: medium

Additional weak hits (30):

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 22.85 out of 32.93

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.65 out of 36.54

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.68 out of 36.54

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 22.67 out of 36.54

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.57 out of 26.33

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.42 out of 33.73

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.22 out of 26.06

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.70 out of 29.25

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.82 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.85 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.79 out of 28.14

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.68 out of 30.22

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.35 out of 39.62

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.43 out of 28.14

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.80 out of 31.73

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.99 out of 30.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.85 out of 26.06

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.81 out of 31.73

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.21 out of 27.78

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.14 out of 32.93

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.03 out of 35.63

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 19.72 out of 27.04

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.93 out of 39.62

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.94 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.11 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.58 out of 18.57

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 20.41 out of 27.04

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.85 out of 28.19

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.62 out of 20.01

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptides

NRPS/PKS products

NRPS/PKS substrates

Type II PKS

TFBS Finder

Core peptide predictions

MZC64_RS26315 - Class III

Cleavage pHMM score: 3.50

RODEO score: 0

Monoisotopic mass: 1079.5 Da

Molecular weight: 1080.2 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1098.2 Da N = 1; 1116.3 Da N = 2; 1134.3 Da N = 3; 1152.3 Da N = 4

MZC64_RS26320 - Class III

Cleavage pHMM score: 3.50

RODEO score: 0

Monoisotopic mass: 1136.5 Da

Molecular weight: 1137.3 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1155.3 Da N = 1; 1173.3 Da N = 2; 1191.3 Da N = 3; 1209.3 Da N = 4

allorf_688904_688996 - Class III

Cleavage pHMM score: 4.00

RODEO score: 0

Monoisotopic mass: 1241.5 Da

Molecular weight: 1242.4 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1260.5 Da N = 1; 1278.5 Da N = 2; 1296.5 Da N = 3

Predicted core structure(s)

For candidate cluster 1, location 600792 - 698907:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - Me-redmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 600792 - 649948:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - Me-redmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 610017 - 651061:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - Me-redmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 614299 - 686776:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - Me-redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MZC64_RS26000: X

AMP-binding (60..450): X

nrpys: Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LASAHTWGLAALFAASSGSYNLYGSTEVSWVSVA
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

MZC64_RS26070: mal - mmal

PKS_AT (511..797): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 66.7%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 186.2
inactive: 89.7
Methoxymalonyl-CoA: 66.2
Methylmalonyl-CoA: 62.3
Isobutyryl-CoA: 48.9
Propionyl-CoA: 37.6
Ethylmalonyl-CoA: 27.2
Benzoyl-CoA: 24.6
Acetyl-CoA: 24.4
2-Methylbutyryl-CoA: 24.2
fatty_acid: 22.3
CHC-CoA: 15.7
3-Methylbutyryl-CoA: 5.0
trans-1,2-CPDA: -2.0

PKS_AT (1492..1789): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 215.4
Ethylmalonyl-CoA: 203.6
Methoxymalonyl-CoA: 181.1
2-Methylbutyryl-CoA: 146.1
trans-1,2-CPDA: 132.4
Propionyl-CoA: 120.5
CHC-CoA: 103.4
Malonyl-CoA: 103.0
Isobutyryl-CoA: 84.7
Benzoyl-CoA: 66.9
3-Methylbutyryl-CoA: 50.8
Acetyl-CoA: 49.8
fatty_acid: 41.9
inactive: 30.2

MZC64_RS26190: mal

PKS_AT (14..292): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 58.3%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 54.2
Methoxymalonyl-CoA: 45.7
Methylmalonyl-CoA: 43.8
Isobutyryl-CoA: 24.2
Propionyl-CoA: 16.8
inactive: 16.5
Benzoyl-CoA: 11.5
fatty_acid: 10.7
2-Methylbutyryl-CoA: 10.0
CHC-CoA: 8.1
trans-1,2-CPDA: 0.0
Ethylmalonyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

MZC64_RS26295: X

AMP-binding (13..259): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ARALHPYGLVLLDG-------------------V
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

MZC64_RS26335: mxmal

PKS_AT (53..290): mxmal

ATSignature: (unknown)

No matches above 50%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 15.5
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

Type II PKS gene cluster prediction details

Cluster section:: 614299 to 686776
Starter unit(s):: hexadienyl-CoA (Score: 908.0; E-value: 0)
propionyl-CoA (Score: 120.0; E-value: 5.73e-32)
Product class(es):: benzoisochromanequinone

MZC64_RS26070: AT (Score: 167.6; E-value: 8.4e-52); AT ABC00730.1_Cosmomycin_propionyl-CoA (Score: 120.0; E-value: 5.73e-32)
MZC64_RS26075: KSIII (Score: 501.7; E-value: 2.2e-153); KSIII AAP85337.1_Hedamycin_hexadienyl-CoA (Score: 498.0; E-value: 0.0)
MZC64_RS26120: GT (Score: 345.2; E-value: 1.5e-105)
MZC64_RS26155: KR C9 (Score: 427.2; E-value: 5.1e-131)
MZC64_RS26160: CYC C5-C14 (Score: 479.6; E-value: 7.5e-147)
MZC64_RS26170: KS (Score: 716.0; E-value: 3.1e-218)
MZC64_RS26175: CLF (Score: 566.3; E-value: 8e-173)
MZC64_RS26180: ACP (Score: 99.8; E-value: 1.5e-31)
MZC64_RS26185: CYC C7-C12 (Score: 419.1; E-value: 1.9e-128)
MZC64_RS26190: AT (Score: 399.0; E-value: 3.7e-122); AT AAP85359.1_Hedamycin_hexadienyl-CoA (Score: 410.0; E-value: 1.5e-147)
MZC64_RS26210: GT (Score: 357.9; E-value: 2.1e-109)

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
DasR	N-acetylglucosamine dependent repressor
RutR	Regulator of the pyrimidine and purine metabolism

NZ_JALMDK010000007.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 58,774 - 126,307 nt. (total: 67,534 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

MZC64_RS26915: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

MZC64_RS27045: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

MZC64_RS27070: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

MZC64_RS27115: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: aminotransferase carbamoyltransferase

MZC64_RS26990: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

MZC64_RS26995: ornithine carbamoyltransferase

smcogs: SMCOG1114: ornithine carbamoyltransferase

Hydrolases: deaminase hydrolase

MZC64_RS26875: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

MZC64_RS26970: ornithine cyclodeaminase

smcogs: SMCOG1158: ornithine cyclodeaminase

Ligases: ligase synthase

MZC64_RS26975: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

MZC64_RS26985: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

MZC64_RS27005: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

MZC64_RS27010: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

MZC64_RS27030: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Translocases: transporter

MZC64_RS27085: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.74	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.73	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.72	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001166.5	0.71	NRPS	HC-toxin	Alternaria jesenskae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.74	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.73	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.72	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001166.5	0.71	NRPS	HC-toxin	Alternaria jesenskae

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.33 out of 24.78

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 29.02 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 29.02 out of 30.11

Confidence: strong

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 18.39 out of 25.31

Confidence: medium

Additional weak hits (8):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.71 out of 29.38

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.21 out of 26.33

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.96 out of 26.33

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 19.67 out of 30.97

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.42 out of 26.85

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.49 out of 25.75

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.86 out of 29.38

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.10 out of 25.88

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 58773 - 126307:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Asn - Ser - X) + (Thr - Asn)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MZC64_RS26985: Asn - Ser - X

Search NORINE for peptide: strict or relaxed

AMP-binding (456..836): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWASFDLPVTSPKLVAGGEFNEYGPTETTVGCSV
Nearest Stachelhaus code(s):: Asn DLTKVGEVGK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1477..1873): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWTTFDASMWEAQLFCAGETNTYGPTECAIDVTA
Nearest Stachelhaus code(s):: Gln DAWQFGLIDK (76% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (2524..2927): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HVISFDVAMDQAEINVGSDLCNYGPTEATVTCLL
Nearest Stachelhaus code(s):: Ahp DVENAGVVTK (53% 8Å match); Orn DVGEIGSVDK (38% 8Å match)
Stachelhaus code match:: 60% (weak)

MZC64_RS27005: X

AMP-binding (9..396): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: YFLAYDASIRDALSVSSGEVNQYGPTECTMTSTR
Nearest Stachelhaus code(s):: Glu DPRSLSQMAK (62% 8Å match)
Stachelhaus code match:: 60% (weak)

MZC64_RS27030: Thr - Asn

Search NORINE for peptide: strict or relaxed

AMP-binding (495..904): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LACHFDFSVWEGNQVFGGEVNMYGITETTVHVTY
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1555..1955): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWCSFDLPVTSPKLIAGGEDNEYGPTETTVGCSW
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CatR	H2O2-responsive repressor
CelR	Cellobiose uptake repressor
LexA variant 2	Repressor of DNA damage response

NZ_JALMDK010000007.1 - Region 2 - lanthipeptide-class-v

Download region GenBank file

Download region SVG

Location: 224,199 - 278,366 nt. (total: 54,168 nt) Show pHMM detection rules used

lanthipeptide-class-v: (APH and HopA1)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

MZC64_RS27610: dehydrogenase

smcogs: SMCOG1072: dehydrogenase

MZC64_RS27715: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

Transferases: acetyltransferase methyltransferase

MZC64_RS27670: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

MZC64_RS27740: methyltransferase

smcogs: SMCOG1248: methyltransferase

MZC64_RS27770: methyltransferase

smcogs: SMCOG1248: methyltransferase

Hydrolases: protease

MZC64_RS27690: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

MZC64_RS27815: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: dehydratase

MZC64_RS27660: L-carnitine dehydratase/bile acid-inducible

smcogs: SMCOG1189: L-carnitine dehydratase/bile acid-inducible

Ligases: synthase

MZC64_RS27705: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001534.3	0.81	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0001535.3	0.62	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0000622.5	0.50	ribosomal	megacin	Bacillus megaterium
BGC0002303.3	0.49	ribosomal	pristinin A3	Streptomyces pristinaespiralis
BGC0000597.4	0.46	ribosomal	SWA-2138	Streptomyces sp. e14
BGC0002536.3	0.46	NRPS	alazopeptin	Kitasatospora azatica KCTC 9699
BGC0000513.3	0.45	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0002130.3	0.45	ribosomal	cacaoidin	Streptomyces cacaoi CA-170360
BGC0000595.3	0.44	ribosomal	SCO-2138	Streptomyces coelicolor A3(2)
BGC0002635.2	0.42	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001534.3	0.81	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0001535.3	0.62	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0000622.5	0.50	ribosomal	megacin	Bacillus megaterium
BGC0002303.3	0.49	ribosomal	pristinin A3	Streptomyces pristinaespiralis
BGC0000597.4	0.46	ribosomal	SWA-2138	Streptomyces sp. e14
BGC0002536.3	0.46	NRPS	alazopeptin	Kitasatospora azatica KCTC 9699
BGC0000513.3	0.45	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0002130.3	0.45	ribosomal	cacaoidin	Streptomyces cacaoi CA-170360
BGC0000595.3	0.44	ribosomal	SCO-2138	Streptomyces coelicolor A3(2)
BGC0002635.2	0.42	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa

Similar known clusters from MIBiG 4.0

Similar subclusters

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.19 out of 20.47

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.37 out of 23.79

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 23.69 out of 30.11

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 25.47 out of 30.11

Confidence: medium

Additional weak hits (16):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.86 out of 21.55

Confidence: weak

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 21.43 out of 32.93

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.84 out of 26.33

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 21.06 out of 30.25

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.74 out of 30.25

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 22.24 out of 32.38

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.76 out of 18.57

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.54 out of 26.85

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.30 out of 25.88

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.25 out of 32.74

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.13 out of 24.78

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.77 out of 23.43

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.73 out of 29.38

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.22 out of 29.25

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 18.42 out of 33.14

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 21.25 out of 36.53

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

No core peptides found.

TFBS definitions

Regulator	Description
CelR	Cellobiose uptake repressor
IolR	Regulator of the inositol catabolism
VqsM	Regulator of quorum-sensing signalling systems

NZ_JALMDK010000007.1 - Region 3 - lanthipeptide-class-i

Download region GenBank file

Download region SVG

Location: 358,658 - 383,003 nt. (total: 24,346 nt) Show pHMM detection rules used

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

MZC64_RS28155: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

MZC64_RS28200: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

MZC64_RS28235: dioxygenase, TauD/TfdA

smcogs: SMCOG1121: dioxygenase, TauD/TfdA

Hydrolases: hydrolase

MZC64_RS28140: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Lyases: cyclase dehydratase

MZC64_RS28185: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

MZC64_RS28190: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.75	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0001289.5	0.57	ribosomal	penisin	Paenibacillus ehimensis
BGC0002640.2	0.57	ribosomal	thalassomonasin A, thalassomonasin B	Thalassomonas actiniarum
BGC0000560.5	0.55	ribosomal	subtilomycin	Bacillus subtilis
BGC0000542.3	0.54	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000541.5	0.54	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000515.4	0.52	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2
BGC0000506.4	0.51	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000559.4	0.50	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0000538.3	0.47	ribosomal	nisin Z	Lactococcus lactis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.75	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0001289.5	0.57	ribosomal	penisin	Paenibacillus ehimensis
BGC0002640.2	0.57	ribosomal	thalassomonasin A, thalassomonasin B	Thalassomonas actiniarum
BGC0000560.5	0.55	ribosomal	subtilomycin	Bacillus subtilis
BGC0000542.3	0.54	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000541.5	0.54	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000515.4	0.52	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2
BGC0000506.4	0.51	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000559.4	0.50	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0000538.3	0.47	ribosomal	nisin Z	Lactococcus lactis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for MZC64_RS28190

MZC64_RS28180

Leader:

MDAFDLDARVSTPAAANGQAQPL

Core:

SITSVITRLACTRTPLCSRFTCQCTQGACTAGCPKR

Core with dehydrations:

DhaIDhbDhaVIDhbRLACysDhbRDhbPLCysDhaRFDhbCysQCysDhbQGACysDhbAGCysPKR

antiSMASH-DB 4.0 matches for MZC64_RS28180

100.0%

NZ_JALMDK010000007.1:

Lanthipeptides: MZC64_RS28180_lanthipeptide

1

SITSVITRLACTRTPLCSRFTCQCTQGACTAGCPKR

36

SITSVITRLACTRTPLCSRFTCQCTQGACTAGCPKR

1

SITSVITRLACTRTPLCSRFTCQCTQGACTAGCPKR

36

41.5%

NZ_CP017248.1and 1 other:

NZ_CP071839.1 Lanthipeptides: S1361_RS32765_lanthipeptide

Lanthipeptides: BFF78_RS08045_lanthipeptide

1

SITSVITRLACTRTPLCSRFTCQCTQGACTAG-CPKR

36

SI+S++TR CTR + T CT CT+G CPK

3

SISSLLTRAVCTRVTCKANCTYVCTT-VCTSGICPKE

38

38.9%

NZ_JACHJE010000002.1:

Lanthipeptides: FHS32_RS05815_lanthipeptide

1

SITSVITRLACTRTPLCSRFTCQCTQG-ACTAGC

33

SI+++ TR +C+R TCQ T+ ACT+ C

1

SISTIATR------TICTRVTCQVTKTCACTSMC

28

with 10 more sequence matches

38.9%

NZ_JALMDK010000003.1:

Lanthipeptides: MZC64_RS14175_lanthipeptide

4

SVITRLACTRTPLCSRFTCQCTQ

26

S+I++LAC+RTP+CS+ TCQC++

1

SIISKLACSRTPVCSKTTCQCSR

23

33.3%

NZ_BNCE01000001.1:

Lanthipeptides: IE320_RS02935_lanthipeptide

1

SITSVITRLACTRTPLCSRFTCQCTQ

26

SI+ + TR CTR TC CTQ

1

SISGIATRTICTRVTCQVTKTCACTQ

26

33.3%

NZ_CP007574.1:

Lanthipeptides: DC74_RS44240_lanthipeptide

1

SITSVITRLACTRTPLCSRFTCQCTQ

26

SI+SV TR CT+ TC CT

1

SISSVATRTVCTKVGCSVTKTCVCTS

26

25.6%

NZ_JAJJMU010000017.1:

Lanthipeptides: LOC25_RS25050_lanthipeptide

1

SITSVITRLACTRTPLCSRFTCQCTQ

26

SI+++ TR CTR TC CT

8

SISTIATRTVCTRVGCSVTKTCACTS

33

25.6%

NZ_JALGBX010000020.1and 1 other:

NZ_JANAVF010000016.1 Lanthipeptides: NLX94_RS30150_lanthipeptide

Lanthipeptides: MTF69_RS28785_lanthipeptide

1

SITSVITRLACTRTPLCSRFTCQCT

25

SI+ + TR CTR + TC CT

8

SISGIATRTVCTRVTCQASKTCVCT

32

21.1%

NZ_CP104794.1:

Lanthipeptides: N6J21_RS16070_lanthipeptide

1

SITSVITRLACTRT

14

SI+S++TR CTR

3

SISSLLTRSLCTRV

16

19.4%

NC_014976.1and 9 others:

NZ_BDDH01000005.1 Lanthipeptides: A7P71_RS15855_lanthipeptideNZ_CP029461.1 Lanthipeptides: DJ572_RS00745_lanthipeptideNZ_CP031675.1 Lanthipeptides: DYS67_RS00910_lanthipeptideNZ_CP045824.1 Lanthipeptides: GII78_RS01205_lanthipeptideNZ_CP046591.1 Lanthipeptides: GO004_RS12840_lanthipeptideNZ_CP049924.1 Lanthipeptides: G4G26_RS01205_lanthipeptideNZ_CP073265.1 Lanthipeptides: KCX77_RS01160_lanthipeptideNZ_CP095736.1 Lanthipeptides: MX663_RS01330_lanthipeptideNZ_JALAUD010000002.1 Lanthipeptides: MOE28_RS05435_lanthipeptide

Lanthipeptides: BSN5_RS12800_lanthipeptide

17

CSRFTCQCTQGACT

30

C FTC C+ G+C+

16

CRTFTCGCSLGSCS

29

19.4%

NZ_CP073819.1:

Lanthipeptides: KFV12_RS00360_lanthipeptide

24

CTQGACTAGCPK

35

CT G C CPK

7

CTPGTCNKECPK

18

16.7%

NZ_ADVG01000001.1:

Lanthipeptides: allorf_1941027_1941140_lanthipeptide

14

TPLCSRFTCQCTQG

27

P C + CQ QG

13

DPGCYEYMCQSQQG

26

16.7%

NZ_NGMO01000003.1and 3 others:

NZ_NGMO01000003.1 Lanthipeptides: allorf_391705_391890_lanthipeptideNZ_NGMO01000003.1 Lanthipeptides: allorf_392307_392492_lanthipeptideNZ_NGMO01000003.1 Lanthipeptides: allorf_391705_391890_lanthipeptide

Lanthipeptides: allorf_392307_392492_lanthipeptide

24

CTQGACTAGCPK

35

CT G C + CP+

3

CTPGTCNSNCPQ

14

MIBiG 4.0 matches for MZC64_RS28180

11.1%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

29

CTAGC

33

CT GC

7

CTPGC

11

11.1%

BGC0001155.5 (AGY49587.1):

Thiopeptide: GE2270

21

TCQCTQGAC

29

+C C G C

1

SCNCVCGFC

9

8.3%

BGC0000563.4 (AEA03263.1):

Lanthipeptide: venezuelin

21

TCQCT

25

TC+C

2

TCECV

6

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 20.03 out of 23.43

Confidence: medium

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 20.34 out of 23.43

Confidence: medium

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 23.59 out of 27.04

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.31 out of 23.79

Confidence: medium

Additional weak hits (1):

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.29 out of 18.57

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

MZC64_RS28180 - Class I

Cleavage pHMM score: 2.70

RODEO score: 26

Monoisotopic mass: 3652.8 Da

Molecular weight: 3655.3 Da

Number of bridges: 6

Alternative weights: (assuming N unmodified Ser/Thr residues); 3673.3 Da N = 1; 3691.3 Da N = 2; 3709.4 Da N = 3; 3727.4 Da N = 4

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 158 - 248; Score: 42.3

TFBS definitions

Regulator	Description
BldD	Development and antibiotic global regulator
DasR	N-acetylglucosamine dependent repressor
IolR	Regulator of the inositol catabolism

NZ_JALMDK010000007.1 - Region 4 - T1PKS

Download region GenBank file

Download region SVG

Location: 393,564 - 539,781 nt. (total: 146,218 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

MZC64_RS28325: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

MZC64_RS28345: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

MZC64_RS28480: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase aminotransferase glycosyltransferase methyltransferase

MZC64_RS28320: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

MZC64_RS28335: methyltransferase

smcogs: SMCOG1248: methyltransferase

MZC64_RS28340: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

MZC64_RS28445: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

MZC64_RS28450: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

MZC64_RS28455: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

MZC64_RS28470: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

MZC64_RS48850: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

Hydrolases: esterase hydrolase

MZC64_RS28285: thioesterase

smcogs: SMCOG1004: thioesterase

MZC64_RS28310: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

MZC64_RS28430: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

MZC64_RS28485: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Lyases: dehydratase

MZC64_RS28295: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthase

MZC64_RS28350: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

MZC64_RS28355: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

MZC64_RS28460: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

MZC64_RS28465: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

MZC64_RS28475: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

MZC64_RS28500: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.74	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.74	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.74	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001164.3	0.66	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.65	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000067.5	0.65	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000041.3	0.62	PKS	oronofacic acid	Pseudomonas syringae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.74	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.74	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.74	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001164.3	0.66	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.65	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000067.5	0.65	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000041.3	0.62	PKS	oronofacic acid	Pseudomonas syringae

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.20 out of 20.97

Confidence: medium

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 24.53 out of 29.51

Confidence: medium

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 25.09 out of 30.12

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.23 out of 19.52

Confidence: medium

Additional weak hits (13):

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.29 out of 25.75

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.89 out of 21.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.77 out of 27.78

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.12 out of 36.54

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.37 out of 29.65

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.14 out of 32.93

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.40 out of 21.84

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.81 out of 21.84

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.58 out of 39.62

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 21.61 out of 32.38

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.45 out of 32.93

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.96 out of 26.06

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.15 out of 24.78

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 4, location 393563 - 539781:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (ccmal - ohmal - Me-ohmal - ccmal - ohmal) + (ccmal - ccmal - ccmal) + (ccmal - Me-redmal) + (Me-ccmal - ohmal - mal - Me-ohmal) + (Me-ccmal) + (ccmal - Me-ccmal - ccmal - ccmal) + (Me-redmal)
Non-colinear ordering:: MZC64_RS28350 + MZC64_RS28355 + MZC64_RS28460 + MZC64_RS28465 + MZC64_RS28470 + MZC64_RS28475 + MZC64_RS28500

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MZC64_RS28350: mal - mal - mmal - mal - mal

PKS_AT (553..848): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 202.8
inactive: 70.6
Methoxymalonyl-CoA: 68.1
Methylmalonyl-CoA: 55.1
Isobutyryl-CoA: 33.0
Ethylmalonyl-CoA: 27.4
Propionyl-CoA: 23.7
Acetyl-CoA: 23.5
Benzoyl-CoA: 23.4
trans-1,2-CPDA: 19.1
fatty_acid: 10.0
CHC-CoA: 8.3
2-Methylbutyryl-CoA: 7.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2268..2559): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 206.3
inactive: 110.7
Methoxymalonyl-CoA: 81.4
Methylmalonyl-CoA: 69.9
Isobutyryl-CoA: 40.5
Propionyl-CoA: 35.5
Ethylmalonyl-CoA: 29.3
Benzoyl-CoA: 23.5
Acetyl-CoA: 23.5
3-Methylbutyryl-CoA: 20.4
trans-1,2-CPDA: 18.0
fatty_acid: 13.4
CHC-CoA: 13.3
2-Methylbutyryl-CoA: 9.6

PKS_AT (3694..3988): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 196.9
Ethylmalonyl-CoA: 185.1
Methoxymalonyl-CoA: 152.7
2-Methylbutyryl-CoA: 128.4
trans-1,2-CPDA: 109.6
Propionyl-CoA: 95.0
Malonyl-CoA: 77.4
CHC-CoA: 68.8
Isobutyryl-CoA: 63.7
Benzoyl-CoA: 57.0
Acetyl-CoA: 34.6
3-Methylbutyryl-CoA: 31.7
fatty_acid: 26.0
inactive: 12.0

PKS_AT (5207..5501): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 214.6
inactive: 114.2
Methoxymalonyl-CoA: 58.4
Methylmalonyl-CoA: 41.8
Propionyl-CoA: 21.3
Isobutyryl-CoA: 18.3
Acetyl-CoA: 15.7
Benzoyl-CoA: 13.1
Ethylmalonyl-CoA: 8.5
fatty_acid: 7.9
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (6620..6910): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 66.7%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 208.1
inactive: 88.1
Methoxymalonyl-CoA: 72.5
Methylmalonyl-CoA: 67.2
Propionyl-CoA: 48.5
Isobutyryl-CoA: 27.0
Acetyl-CoA: 26.4
Benzoyl-CoA: 23.5
2-Methylbutyryl-CoA: 21.8
Ethylmalonyl-CoA: 20.2
fatty_acid: 17.8
CHC-CoA: 16.1
trans-1,2-CPDA: 9.8
3-Methylbutyryl-CoA: 0.0

MZC64_RS28355: mal - mal - mal

PKS_AT (545..838): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 201.0
inactive: 92.3
Methoxymalonyl-CoA: 62.2
Methylmalonyl-CoA: 56.9
Acetyl-CoA: 26.8
Isobutyryl-CoA: 26.6
Ethylmalonyl-CoA: 23.5
Propionyl-CoA: 22.5
Benzoyl-CoA: 16.7
2-Methylbutyryl-CoA: 14.4
fatty_acid: 12.7
CHC-CoA: 11.4
trans-1,2-CPDA: 8.7
3-Methylbutyryl-CoA: 3.4

PKS_AT (2205..2503): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 204.9
inactive: 93.9
Methylmalonyl-CoA: 64.4
Methoxymalonyl-CoA: 63.8
Isobutyryl-CoA: 40.6
Acetyl-CoA: 36.0
Benzoyl-CoA: 30.6
Ethylmalonyl-CoA: 27.5
Propionyl-CoA: 21.0
trans-1,2-CPDA: 20.2
fatty_acid: 14.0
2-Methylbutyryl-CoA: 10.6
CHC-CoA: 9.1
3-Methylbutyryl-CoA: 0.0

PKS_AT (3816..4118): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 202.6
inactive: 87.1
Methoxymalonyl-CoA: 65.3
Methylmalonyl-CoA: 52.9
Isobutyryl-CoA: 27.7
Acetyl-CoA: 21.3
Propionyl-CoA: 19.7
Benzoyl-CoA: 19.7
Ethylmalonyl-CoA: 15.4
fatty_acid: 13.1
trans-1,2-CPDA: 9.6
3-Methylbutyryl-CoA: 6.1
2-Methylbutyryl-CoA: 5.5
CHC-CoA: 3.5

MZC64_RS28455: mmal - mmal

PKS_AT (589..864): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 70.8%; Malonyl-CoA: 66.7%; Methoxymalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 112.1
Methoxymalonyl-CoA: 92.4
Ethylmalonyl-CoA: 85.1
Malonyl-CoA: 64.4
Propionyl-CoA: 57.2
2-Methylbutyryl-CoA: 56.2
Isobutyryl-CoA: 53.3
trans-1,2-CPDA: 41.2
3-Methylbutyryl-CoA: 32.0
Benzoyl-CoA: 29.6
CHC-CoA: 22.5
inactive: 18.9
Acetyl-CoA: 17.9
fatty_acid: 14.8

PKS_AT (995..1286): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 211.4
Ethylmalonyl-CoA: 211.1
Methoxymalonyl-CoA: 179.3
2-Methylbutyryl-CoA: 149.0
trans-1,2-CPDA: 114.4
Propionyl-CoA: 108.4
CHC-CoA: 103.1
Malonyl-CoA: 99.7
Isobutyryl-CoA: 88.3
Benzoyl-CoA: 58.5
Acetyl-CoA: 51.8
3-Methylbutyryl-CoA: 42.0
fatty_acid: 36.1
inactive: 23.5

MZC64_RS28460: mal - mmal

PKS_AT (574..873): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 62.5%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 171.4
inactive: 74.9
Methoxymalonyl-CoA: 49.9
Methylmalonyl-CoA: 44.4
Isobutyryl-CoA: 25.8
Propionyl-CoA: 19.9
3-Methylbutyryl-CoA: 10.6
2-Methylbutyryl-CoA: 9.0
Ethylmalonyl-CoA: 8.1
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0

PKS_AT (2272..2564): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 200.1
Ethylmalonyl-CoA: 180.8
Methoxymalonyl-CoA: 166.0
2-Methylbutyryl-CoA: 119.2
Propionyl-CoA: 103.6
trans-1,2-CPDA: 94.6
Malonyl-CoA: 91.0
Isobutyryl-CoA: 86.3
CHC-CoA: 74.3
Benzoyl-CoA: 53.6
fatty_acid: 44.4
Acetyl-CoA: 43.2
3-Methylbutyryl-CoA: 40.6
inactive: 22.2

MZC64_RS28465: mmal - mal - mal - mmal

PKS_AT (553..845): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 193.3
Ethylmalonyl-CoA: 174.1
Methoxymalonyl-CoA: 158.1
2-Methylbutyryl-CoA: 107.0
Propionyl-CoA: 105.7
trans-1,2-CPDA: 91.0
Isobutyryl-CoA: 90.6
Malonyl-CoA: 79.6
CHC-CoA: 77.2
Benzoyl-CoA: 51.0
Acetyl-CoA: 40.0
fatty_acid: 36.3
3-Methylbutyryl-CoA: 33.6
inactive: 28.6

PKS_AT (2138..2442): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.2
inactive: 81.0
Methoxymalonyl-CoA: 76.2
Methylmalonyl-CoA: 64.8
Isobutyryl-CoA: 55.3
Acetyl-CoA: 38.6
Ethylmalonyl-CoA: 35.1
Propionyl-CoA: 30.3
Benzoyl-CoA: 25.4
fatty_acid: 17.6
CHC-CoA: 17.1
2-Methylbutyryl-CoA: 15.4
3-Methylbutyryl-CoA: 10.8
trans-1,2-CPDA: 9.2

PKS_AT (3492..3773): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 70.8%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 212.4
inactive: 111.5
Methoxymalonyl-CoA: 77.2
Methylmalonyl-CoA: 68.3
Isobutyryl-CoA: 43.8
Propionyl-CoA: 37.6
Acetyl-CoA: 34.1
Ethylmalonyl-CoA: 29.3
Benzoyl-CoA: 25.4
fatty_acid: 20.4
trans-1,2-CPDA: 20.3
2-Methylbutyryl-CoA: 19.5
CHC-CoA: 13.6
3-Methylbutyryl-CoA: 8.3

PKS_AT (4484..4777): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 198.8
Ethylmalonyl-CoA: 187.3
Methoxymalonyl-CoA: 160.0
2-Methylbutyryl-CoA: 106.7
Malonyl-CoA: 100.6
trans-1,2-CPDA: 92.4
Propionyl-CoA: 86.9
CHC-CoA: 77.3
Isobutyryl-CoA: 57.0
Benzoyl-CoA: 43.0
Acetyl-CoA: 36.9
fatty_acid: 31.8
inactive: 30.9
3-Methylbutyryl-CoA: 21.8

MZC64_RS28470: mmal

PKS_AT (552..845): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 202.0
Ethylmalonyl-CoA: 179.1
Methoxymalonyl-CoA: 164.2
2-Methylbutyryl-CoA: 129.6
Malonyl-CoA: 116.1
Propionyl-CoA: 110.0
trans-1,2-CPDA: 90.3
CHC-CoA: 77.6
Isobutyryl-CoA: 76.3
Benzoyl-CoA: 52.6
Acetyl-CoA: 40.1
inactive: 39.3
3-Methylbutyryl-CoA: 32.7
fatty_acid: 30.8

MZC64_RS28475: mal - mmal - mal - mal

PKS_AT (543..820): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 62.5%; Ethylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 191.3
inactive: 92.8
Methoxymalonyl-CoA: 69.8
Methylmalonyl-CoA: 63.0
Acetyl-CoA: 38.6
Propionyl-CoA: 36.8
Ethylmalonyl-CoA: 32.3
Isobutyryl-CoA: 30.2
CHC-CoA: 18.4
2-Methylbutyryl-CoA: 16.4
fatty_acid: 15.4
Benzoyl-CoA: 14.5
trans-1,2-CPDA: 10.5
3-Methylbutyryl-CoA: 0.0

PKS_AT (2232..2530): mmal

ATSignature: Methoxymalonyl-CoA, Methylmalonyl-CoA, Ethylmalonyl-CoA

Top 3 matches:: Methoxymalonyl-CoA: 79.2%; Methylmalonyl-CoA: 79.2%; Ethylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 171.0
Ethylmalonyl-CoA: 156.4
Methoxymalonyl-CoA: 136.4
2-Methylbutyryl-CoA: 128.2
trans-1,2-CPDA: 102.2
Propionyl-CoA: 88.5
CHC-CoA: 84.0
Malonyl-CoA: 81.1
Isobutyryl-CoA: 74.0
Benzoyl-CoA: 51.4
3-Methylbutyryl-CoA: 48.2
Acetyl-CoA: 45.9
inactive: 30.3
fatty_acid: 14.2

PKS_AT (3886..4188): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 66.7%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 199.5
inactive: 93.7
Methoxymalonyl-CoA: 72.7
Methylmalonyl-CoA: 53.0
Isobutyryl-CoA: 31.9
Propionyl-CoA: 23.6
Ethylmalonyl-CoA: 21.7
3-Methylbutyryl-CoA: 19.1
Benzoyl-CoA: 15.5
CHC-CoA: 15.4
Acetyl-CoA: 14.6
fatty_acid: 12.4
trans-1,2-CPDA: 8.7
2-Methylbutyryl-CoA: -1.3

PKS_AT (5570..5870): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 180.8
inactive: 80.4
Methoxymalonyl-CoA: 50.5
Methylmalonyl-CoA: 40.9
Propionyl-CoA: 31.1
Ethylmalonyl-CoA: 18.7
2-Methylbutyryl-CoA: 15.6
Isobutyryl-CoA: 11.3
Benzoyl-CoA: 11.2
fatty_acid: 10.6
CHC-CoA: 9.3
trans-1,2-CPDA: 8.3
Acetyl-CoA: 7.3
3-Methylbutyryl-CoA: 0.0

MZC64_RS28500: mmal

PKS_AT (555..851): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 190.3
Ethylmalonyl-CoA: 167.1
Methoxymalonyl-CoA: 155.5
2-Methylbutyryl-CoA: 139.2
Propionyl-CoA: 108.9
trans-1,2-CPDA: 103.1
CHC-CoA: 87.6
Malonyl-CoA: 83.6
Isobutyryl-CoA: 59.1
Benzoyl-CoA: 39.9
fatty_acid: 34.7
Acetyl-CoA: 31.2
3-Methylbutyryl-CoA: 27.0
inactive: 22.2

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
CcpA	Catabolite control protein A
DosR	Hypoxia stress response regulator
OsdR	Development and stress management regulator

NZ_JALMDK010000008.1 - Region 1 - lassopeptide

Download region GenBank file

Download region SVG

Location: 26,026 - 48,829 nt. (total: 22,804 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lasso peptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

MZC64_RS28660: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: glycosyltransferase

MZC64_RS28650: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

Hydrolases: hydrolase

MZC64_RS28670: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

MZC64_RS28695: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001539.4	0.68	ribosomal	cattlecin	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0001493.5	0.65	ribosomal	achromosin	Streptomyces achromogenes subsp. achromogenes
BGC0001551.4	0.63	ribosomal	citrulassin E	Streptomyces glaucescens
BGC0001548.4	0.63	ribosomal	citrulassin A	Streptomyces albulus
BGC0002053.3	0.61	ribosomal	ulleungdin	Streptomyces sp.
BGC0000307.4	0.60	NRPS	AbT1	Aureobasidium pullulans
BGC0001507.3	0.60	ribosomal	anantin C	Streptomyces olindensis
BGC0002174.2	0.60	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0001240.4	0.60	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001261.3	0.60	NRPS	AM-toxin	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001539.4	0.68	ribosomal	cattlecin	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0001493.5	0.65	ribosomal	achromosin	Streptomyces achromogenes subsp. achromogenes
BGC0001551.4	0.63	ribosomal	citrulassin E	Streptomyces glaucescens
BGC0001548.4	0.63	ribosomal	citrulassin A	Streptomyces albulus
BGC0002053.3	0.61	ribosomal	ulleungdin	Streptomyces sp.
BGC0000307.4	0.60	NRPS	AbT1	Aureobasidium pullulans
BGC0001507.3	0.60	ribosomal	anantin C	Streptomyces olindensis
BGC0002174.2	0.60	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0001240.4	0.60	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001261.3	0.60	NRPS	AM-toxin	Alternaria alternata

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lasso peptide predictions

Lassopeptides - No core peptides found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (6):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.47 out of 26.33

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.19 out of 28.19

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.91 out of 35.63

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.38 out of 27.18

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.34 out of 28.19

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.28 out of 27.18

Confidence: weak

Lasso peptides

RREFinder

TFBS Finder

Core peptide predictions

No core peptides found.

RRE predictions

: RRE type: Stand_Alone_Lasso_RRE; Location: 3 - 84; Score: 77.2; RRE type: Lasso_Fused_RRE; Location: 0 - 57; Score: 26.3

TFBS definitions

Regulator	Description

NZ_JALMDK010000008.1 - Region 2 - lanthipeptide-class-v

Download region GenBank file

Download region SVG

Location: 303,862 - 351,358 nt. (total: 47,497 nt) Show pHMM detection rules used

lanthipeptide-class-v: (APH and HopA1)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

MZC64_RS29995: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

MZC64_RS30020: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

MZC64_RS30120: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: aminotransferase methyltransferase sulfhydrylase

MZC64_RS29935: O-succinylhomoserine sulfhydrylase

smcogs: SMCOG1168: O-succinylhomoserine sulfhydrylase

MZC64_RS29940: aminotransferase

smcogs: SMCOG1019: aminotransferase

MZC64_RS30045: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

Hydrolases: hydrolase

MZC64_RS30010: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Ligases: ligase

MZC64_RS29980: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002099.3	0.56	ribosomal	biarylitide YFH	Planomonospora sp. ID107089
BGC0002084.4	0.56	ribosomal	biarylitide YYH	Planomonospora sp. ID82291
BGC0001310.5	0.51	PKS	naringenin	Streptomyces clavuligerus ATCC 27064
BGC0002127.3	0.47	PKS	flaviolin, 1,3,6,8-tetrahydroxynaphthalene	Streptomyces coelicolor A3(2)
BGC0001224.5	0.47	other (other)	reductasporine	uncultured bacterium
BGC0002303.3	0.46	ribosomal	pristinin A3	Streptomyces pristinaespiralis
BGC0002405.2	0.46	saccharide	mid-chain acyl sugars	Solanum lycopersicum
BGC0001485.4	0.46	other (other)	chuangxinmycin	Actinoplanes tsinanensis
BGC0000265.5	0.45	PKS	rhodomycin	Streptomyces purpurascens
BGC0001986.3	0.45	other (other)	purincyclamide	Streptomyces chrestomyceticus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002099.3	0.56	ribosomal	biarylitide YFH	Planomonospora sp. ID107089
BGC0002084.4	0.56	ribosomal	biarylitide YYH	Planomonospora sp. ID82291
BGC0001310.5	0.51	PKS	naringenin	Streptomyces clavuligerus ATCC 27064
BGC0002127.3	0.47	PKS	flaviolin, 1,3,6,8-tetrahydroxynaphthalene	Streptomyces coelicolor A3(2)
BGC0001224.5	0.47	other (other)	reductasporine	uncultured bacterium
BGC0002303.3	0.46	ribosomal	pristinin A3	Streptomyces pristinaespiralis
BGC0002405.2	0.46	saccharide	mid-chain acyl sugars	Solanum lycopersicum
BGC0001485.4	0.46	other (other)	chuangxinmycin	Actinoplanes tsinanensis
BGC0000265.5	0.45	PKS	rhodomycin	Streptomyces purpurascens
BGC0001986.3	0.45	other (other)	purincyclamide	Streptomyces chrestomyceticus

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 18.15 out of 18.46

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.34 out of 23.79

Confidence: medium

Additional weak hits (15):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.56 out of 26.85

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.12 out of 21.84

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.90 out of 20.97

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.94 out of 32.74

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.29 out of 26.06

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.63 out of 25.75

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.32 out of 33.14

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.66 out of 29.65

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.98 out of 29.25

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.60 out of 30.98

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.19 out of 33.14

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.16 out of 29.65

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.44 out of 26.06

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.83 out of 24.78

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.76 out of 20.01

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

No core peptides found.

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
RpoN	Regulator of nitrogen assimilation and virulence

NZ_JALMDK010000009.1 - Region 1 - lassopeptide

Download region GenBank file

Download region SVG

Location: 308,283 - 330,858 nt. (total: 22,576 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lasso peptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase peptidase

MZC64_RS32175: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

MZC64_RS32185: methionine aminopeptidase

smcogs: SMCOG1172: methionine aminopeptidase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002006.2	0.60	ribosomal	albusnodin	Streptomyces albus
BGC0001506.5	0.44	ribosomal	anantin B1, anantin B2	Streptomyces sp. NRRL S-146
BGC0001548.4	0.43	ribosomal	citrulassin A	Streptomyces albulus
BGC0002053.3	0.43	ribosomal	ulleungdin	Streptomyces sp.
BGC0000528.5	0.42	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002074.2	0.42	ribosomal	huascopeptin	Streptomyces huasconensis
BGC0001507.3	0.41	ribosomal	anantin C	Streptomyces olindensis
BGC0002316.2	0.41	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0001552.3	0.41	ribosomal	citrulassin F	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001550.3	0.40	ribosomal	citrulassin D	Streptomyces katrae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002006.2	0.60	ribosomal	albusnodin	Streptomyces albus
BGC0001506.5	0.44	ribosomal	anantin B1, anantin B2	Streptomyces sp. NRRL S-146
BGC0001548.4	0.43	ribosomal	citrulassin A	Streptomyces albulus
BGC0002053.3	0.43	ribosomal	ulleungdin	Streptomyces sp.
BGC0000528.5	0.42	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002074.2	0.42	ribosomal	huascopeptin	Streptomyces huasconensis
BGC0001507.3	0.41	ribosomal	anantin C	Streptomyces olindensis
BGC0002316.2	0.41	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0001552.3	0.41	ribosomal	citrulassin F	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001550.3	0.40	ribosomal	citrulassin D	Streptomyces katrae

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lasso peptide predictions

Lassopeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.80 out of 20.47

Confidence: medium

Additional weak hits (15):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.53 out of 27.78

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.23 out of 27.55

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.49 out of 30.97

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.57 out of 26.33

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.47 out of 26.33

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.79 out of 26.85

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 19.52 out of 33.14

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.63 out of 23.43

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 19.59 out of 33.73

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.49 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.37 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.44 out of 28.19

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.26 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.80 out of 28.19

Confidence: weak

Lasso peptides

TFBS Finder

Core peptide predictions

No core peptides found.

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems