LOCUS       NZ_JALMDK010000025     22300 bp    DNA     linear   CON 13-SEP-2024
DEFINITION  Crossiella sp. S99.2 NODE_25_length_62816_cov_144.261926, whole
            genome shotgun sequence.
ACCESSION   NZ_JALMDK010000025
VERSION     NZ_JALMDK010000025.1
KEYWORDS    WGS; RefSeq.
SOURCE      Crossiella sp. S99.2
  ORGANISM  Crossiella sp. S99.2
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Crossiella.
REFERENCE   1  (bases 1 to 62816)
  AUTHORS   Gonzalez-Pimentel,J.L.
  TITLE     From North to South: A Spanish map of bacteria producing bioactive
            compounds in show caves
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 62816)
  AUTHORS   Gonzalez-Pimentel,J.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-APR-2022) Agroquimica, Microbiologia Ambiental y
            Conservacion de Suelos, Instituto de Recursos Naturales y
            Agrobiologia, Av. Reina Mercedes 10, Sevilla 41012, Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: SPAdes v. 3.14.1
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023094165.1-RS_2024_09_13
            Annotation Date                   :: 09/13/2024 06:26:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,481
            CDSs (total)                      :: 9,422
            Genes (coding)                    :: 9,365
            CDSs (with protein)               :: 9,365
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 8 of 57
            Pseudo Genes (incomplete)         :: 50 of 57
            Pseudo Genes (internal stop)      :: 4 of 57
            Pseudo Genes (multiple problems)  :: 4 of 57
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:32:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 40516
            Orig. end                         :: 62816
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALMDK010000025.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..22300
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lassopeptide"
                     /region_number="1"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..22300
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22300
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[10000:12554](+)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12554
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     misc_feature    complement(1027..1042)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.77"
                     /tool="antismash"
     gene            1138..3078
                     /locus_tag="MZC64_RS45990"
                     /old_locus_tag="MZC64_46000"
     CDS             1138..3078
                     /EC_number="1.4.3.21"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF008559.0"
                     /locus_tag="MZC64_RS45990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MZC64_46000"
                     /product="primary-amine oxidase"
                     /protein_id="WP_247765967.1"
                     /transl_table=11
                     /translation="MTTAQPAAVPALHPADPLTAAEIDTVRDLITRTHPDLALRFSLIA
                     AVPPTMQDNAAFRRGQGQGTRWATVIANDHQGQTYLGEVCLSPPRISLWQVRPGLQPAI
                     TSDELMTLSEIVTSDARAVAALARHGIIDLELLQCDPWTAGNLPIKGVDPSRRIVRATL
                     YQRHFPQDNGYAHPLPLIVTVDLTQRNVLHLEDTGPAPIPADCANYLTEHLGPPRTDIR
                     PLTITQPEGPSFTVDGGVITWHNWRAHIALHPVDGLVLSDVSYQDGDRSRRVLHHLALA
                     EMVVPYGDPHPLFSWRNAFDVGEYHLGRQVNSLRLGCDCVGEIVYLDATIPDHDGRPEA
                     KPNAICLHEEDASIAWRHNNFRWEDRPVVRRARRLVLSSWFTVGNYEYCVEYSLHYDGR
                     IECTVKLGGIIQTRAALPGEPDGYGGRITPELMAPIHQHFFAFRLVPEIDGAEITVHEV
                     DAVAAPPGPDNPAGNAFRTQTTLIESEAMSGRVTDPARGRMWRVSSATARNAAGAPTAY
                     SLHLNPAPRLLAAPESSIAHRAAFATKDLWITQHRLSEWYPAGDFVNQSNGGGGIPDWI
                     RQQRSLTDTTLTLWPVLGTLHSPSTEDWPVMPTAQTGFALTPNGFFDRNPMLDLPASAG
                     TTPSHDRSACH"
     gene            3275..3529
                     /locus_tag="MZC64_RS45995"
                     /old_locus_tag="MZC64_46005"
     CDS             3275..3529
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MZC64_RS45995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MZC64_46005"
                     /product="hypothetical protein"
                     /protein_id="WP_247765966.1"
                     /transl_table=11
                     /translation="MVIDLVCSEHSTVDRYAVTVELATPEPTCIRSGADQEAVASRWAS
                     ITARDSTTGARFSGLVNIRDRHIASWRLHPPETASPGSD"
     gene            3706..>4509
                     /locus_tag="MZC64_RS46000"
                     /old_locus_tag="MZC64_46010"
                     /pseudo=""
     CDS             3706..>4509
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1292: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF016550.5"
                     /locus_tag="MZC64_RS46000"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="MZC64_46010"
                     /product="SAM-dependent methyltransferase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAAAIGTEIRRGTPGDQRWPYAPGVSRARLLNGCLGGALTTPADR
                     TLATALDAADPTWGAAAAITATFVEETLLAGARAGIRQVLQIGNGAPVPTTMPHEQFAA
                     HHRGSTNSIRYIYALEDPVEAALVCEELIASTREPAPVPMDARVVHVADSRLTTILADV
                     RACQALGDQEPLLMLLPLTGLNDAEFRELITHARTALPAGSVIAIAATGFGCTPTAKEI
                     LVAKNMGRIYRDAGLSFPLTTWAELEVALRDWRLLEPGIVAQTRWR"
     gene            5884..6372
                     /locus_tag="MZC64_RS46005"
                     /old_locus_tag="MZC64_46015"
     CDS             5884..6372
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MZC64_RS46005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MZC64_46015"
                     /product="hypothetical protein"
                     /protein_id="WP_247765964.1"
                     /transl_table=11
                     /translation="MAGSLPLPIQSHSQAPGNAEHTAVPDTEWCREQLDRSLLRYRRAL
                     RTGAPTCLLTALILLGFGTRIHREVHKTVGGLMAVTGLVVIAIWLGWWWLRWMVLSTDR
                     SMYRLSVAANEAVAWSGLEPRHLRQGLMYTRERGGVYQFYGPLPDHALHAESSSTQDG"
     gene            6466..8784
                     /locus_tag="MZC64_RS46010"
                     /old_locus_tag="MZC64_46020"
     CDS             6466..8784
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF024589.5"
                     /locus_tag="MZC64_RS46010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MZC64_46020"
                     /product="ATP-binding protein"
                     /protein_id="WP_247797801.1"
                     /transl_table=11
                     /translation="MTITTGPSAIQDAAPAVHGHAKREAVLRWARILDQAKPASVIPEP
                     RQRPSQFPTAPQPLLGRDRDLALLDELAALHRAQPTSATVVISGEVGIGKTALALTWGQ
                     RNQQQFPHGAIWLDLADYRHGTHAALDATAVLEALLRALTIPAWRIDAAHTRALKVELL
                     HNATAGQHLLIVVDNAEATDDLLGLLPSSTTCVVLITTAVGPPGLWGLREAKHHVLTPL
                     DDTAATELAAGILGASRIEAADQAWEQILTHCAGNPLALRLAAVTIATRRPSSLTWAAR
                     RLTGETGTCRATLGTARSTDVVASATALALQGAGPLLVRRVRRLAEYPGHELTPPVAAA
                     VLGCGTSTEGGEILQRLTDRGLLTMIHPNHYRIPRALARTIRHATPPLSAASQAAAVAA
                     GLRQLARTAVAAIDVLGGSGDSSLRTPVRDGSTAADLFDSPLQALAWLEVHRRDLQRAV
                     TITVQLRELRSIVWPLHYALSRLCALQGGAADWIRIYQKVLPLACESGMQRRQSEVRFG
                     LAMARLVAPRPYRAGSADAGLPASAHLDLASRLASITGSRFLSLPEQWNGFLPYLSSRW
                     ADPRVAEKLHCTAAAIFVRDHNARMAHVHNIGLAESCAATGRSAQALALLNTASQAFAL
                     MDDRAAQVRVLITMAAVHLGLGKAHNAADALARARMLLSPESSRAEHAAVEGSTAIVAD
                     TMGQHSRVRSARAALAQLLPASGSTPAITAVLETLEREGNRTAASARQNTGPVPHAVAA
                     RHDRPPGVSQQHHTSTVLA"
     gene            9067..9420
                     /locus_tag="MZC64_RS46015"
                     /old_locus_tag="MZC64_46025"
     CDS             9067..9420
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MZC64_RS46015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MZC64_46025"
                     /product="hypothetical protein"
                     /protein_id="WP_247765962.1"
                     /transl_table=11
                     /translation="MTTLRPTSLRWATLLGSLLAMIALTPLILYWTGVAAMGVASCSMT
                     TDPAHQGICSVPMQFLLLILPSAVWVLSFVVMLVCGIVAISHRRRLTWWIAGPALATGF
                     ALGVSVGLGLWLG"
     misc_feature    complement(9440..9455)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.52"
                     /tool="antismash"
     gene            9842..9988
                     /locus_tag="MZC64_RS46020"
                     /old_locus_tag="MZC64_46030"
     CDS             9842..9988
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF033521.1"
                     /locus_tag="MZC64_RS46020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MZC64_46030"
                     /product="lasso RiPP family leader peptide-containing
                     protein"
                     /protein_id="WP_247765961.1"
                     /transl_table=11
                     /translation="MEATTFPTDAIKEAPRYEPPEVIALGGVVELTQGSAFDDTMEKRS
                     YYF"
     gene            10001..11833
                     /locus_tag="MZC64_RS46025"
                     /old_locus_tag="MZC64_46035"
                     /pseudo=""
     CDS             10001..11833
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012936.5"
                     /locus_tag="MZC64_RS46025"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="MZC64_46035"
                     /product="albusnodin/ikarugamycin family macrolactam
                     cyclase"
                     /pseudo=""
                     /sec_met_domain="Asn_synthase (E-value: 9.2e-11, bitscore:
                     33.6, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWLAETVGRDALHVPPARACPDLTAVAADWIRLWHTRSGPPGGLR
                     KVDVPGCRLLVLGYCPADDRELTTIGEQVARGGDSALASVGGSSVVIAVRRDDAVIVGD
                     LAGQRTVFYTRTPNGRTVLGSHCSHLARTVSTGVDKGWLATRLILPRASDVWAEGSPWT
                     GVQALRPGWGLRLRRTGASSVHQVHTLPTPETEQGDAAVVLRTALRQAVELRVRTAQVS
                     SVDLSGGLDSSTVATLAAKTAPGRVPA"
     misc_feature    11528..11530
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            11866..12099
                     /locus_tag="MZC64_RS46030"
                     /old_locus_tag="MZC64_46040"
     CDS             11866..12099
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017239.5"
                     /locus_tag="MZC64_RS46030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MZC64_46040"
                     /product="PqqD family protein"
                     /protein_id="WP_247765960.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 1.6e-11,
                     bitscore: 35.9, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 6e-11, bitscore: 34.3,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIEGGTMIVLAERSGQYYQGSSTARAMWCALQTHAGDLSPAAQSV
                     ATEFDVPVSQVRADLETFVGRLLELSLLEVTA"
     aSDomain        11878..12090
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_MZC64_RS46030_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="9.00E-12"
                     /identifier="RREFam002.1"
                     /label="MZC64_RS46030"
                     /locus_tag="MZC64_RS46030"
                     /protein_end="75"
                     /protein_start="4"
                     /score="35.9"
                     /tool="antismash"
                     /translation="GTMIVLAERSGQYYQGSSTARAMWCALQTHAGDLSPAAQSVATEF
                     DVPVSQVRADLETFVGRLLELSLLEV"
     misc_feature    12064..12066
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            12102..12554
                     /locus_tag="MZC64_RS46035"
                     /old_locus_tag="MZC64_46045"
     CDS             12102..12554
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /locus_tag="MZC64_RS46035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MZC64_46045"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_247765997.1"
                     /sec_met_domain="PF13471 (E-value: 7.3e-22, bitscore: 69.3,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPMAMRQKRPPGLLATSIGACAGLAAWAVLRLIRFDRLTAAAEAL
                     ARTTQRAATAEETQRALDMVTAGARWIPGRVACLERSLAAFVILAVCRRGVSWCIGVRT
                     PPITAHAWLADDAGTPLGEPDSTTGCRPLRVISRLPGTTRGSWRTS"
     gene            12551..13552
                     /locus_tag="MZC64_RS46040"
                     /old_locus_tag="MZC64_46050"
     CDS             12551..13552
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PCMT"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013315.5"
                     /locus_tag="MZC64_RS46040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MZC64_46050"
                     /product="methyltransferase domain-containing protein"
                     /protein_id="WP_247765959.1"
                     /sec_met_domain="PCMT (E-value: 1.4e-28, bitscore: 91.7,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNGIDVDYQVWVHTSEGEVIPQSTAPATIERMLGLLAPAPGAAVL
                     EIGTGSGYTGAVLSHLVGDSGRVVSLDVDADLVIRARGLHASANHRNIEVHVHNGFQGW
                     APGAPYDAVIGWTTAPVLPRAWVEQAAPTAVLVAPVKIAEIAHANAVILCTLHQGAPVN
                     PALHPGSFIDMHHEVIEELGRPVRFVDSELRDDAGSAWWLSATALRDHAMRESATALLE
                     QIRLAEPVTGFLPQDRQTMESFAAFVLSTTTSPGSTGGPGLGRCIGVVHAQGAAFVTSH
                     GHLLAAGGTEAVEELGELRRQWQHRGRPRLQDLEVVLTEVAEGWAVRAQCGA"
     gene            complement(13760..13888)
                     /locus_tag="MZC64_RS48445"
     CDS             complement(13760..13888)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MZC64_RS48445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_282106995.1"
                     /transl_table=11
                     /translation="MRSGSGTLRAVAQALHAPREKDSVLEIIGARPRALLRVSAQD"
     gene            14979..16331
                     /locus_tag="MZC64_RS46045"
                     /old_locus_tag="MZC64_46055"
     CDS             14979..16331
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024949.5"
                     /locus_tag="MZC64_RS46045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MZC64_46055"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_247765958.1"
                     /transl_table=11
                     /translation="MGDRLALRQRREACGYSQEQLAGEVGVTPRTVGRWERGQSNPQPW
                     HRPRLAALLRLGIEQLGVLLTENEPGLTGRDLASGTHTALPETLSYGHTVIIRGQRETT
                     ECDDVNRRDLLRLFSFAGAGLAFPAAADAVDWDRLAYHSTHALRPDPETLDQYEKLNLR
                     LWREYSDAVSKMDMLAVVRHQLNELTSSLQNSPEGPSRRRLSAYTADLFQLAGEILFDA
                     NRYTDAAQCYSLATEAGKQAQDYDLWACALVRHSFIDLYDRHFGKALPLLDAAMRCAQR
                     GDPSLPTRHWVSVVRGQALAGLGQERACRQAFDIADGVHDIDGRHRTSGWLRFNGTRLP
                     EERGASLVELGIPALAEASLTEALRGPLSTRRRGTVLADLATVGFLSRDLDQMALSARH
                     ALEAAQQTGSGVIERKLLGLRERFIPLLGEKRISVLDNQITHLFPTGSTHR"
     misc_feature    15159..15161
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            16345..16713
                     /locus_tag="MZC64_RS46050"
                     /old_locus_tag="MZC64_46060"
     CDS             16345..16713
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010316234.1"
                     /locus_tag="MZC64_RS46050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MZC64_46060"
                     /product="hypothetical protein"
                     /protein_id="WP_247765957.1"
                     /transl_table=11
                     /translation="MQHEPGEFFRQTWIDAVNEHYPGEPKSSYVAPWPETREWEQRCAA
                     AVEGQLRDFLTRSDGHAAQLTQEQKGQYVTLCWLAQVYRHVDDPKPAYVAGWGDMQEWH
                     RRVNISIFLRFEQEFQAS"
     gene            17559..18800
                     /locus_tag="MZC64_RS46055"
                     /old_locus_tag="MZC64_46065"
     CDS             17559..18800
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1237: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /locus_tag="MZC64_RS46055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MZC64_46065"
                     /product="IS110 family transposase"
                     /protein_id="WP_247765956.1"
                     /transl_table=11
                     /translation="MPRCFAGIDWSEGLNDVAVVDDSGEIIASARIPETPEGITECLRL
                     LAKANPSSRHRRRDVPIAIETGTGLLVDALVSRGQTVYAINPAVVARYRGRLHPTRQKS
                     DRRDAALLANILRTDLIHHRPLPVNTPAATAIAVLVREYQSAARARQRHFNRLRSQLRE
                     YFPAAITAWQELPNGLLRPEARALLAIAPTPALAARLSRTRVRTTLAEAGRTRLLDDQA
                     DRLFDHFRIRQLRTHPVVEETMGIRTLAQLDALNHADHTATELAEHAGTAFEAHPHSAI
                     YRSFPGVGPLIALRLFAELGDDNTRFATARGLRAYAGAAPLTWASGSGSSVSYRRIANK
                     YLMATGHVWAFTSLTKSPGCRDHYDRRRAAGDAHAAALRNLFGRLITCLHHCLNHNELY
                     NEATAFATAVSEAA"
     gene            19224..21041
                     /locus_tag="MZC64_RS46060"
                     /old_locus_tag="MZC64_46070"
     CDS             19224..21041
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MZC64_RS46060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MZC64_46070"
                     /product="hypothetical protein"
                     /protein_id="WP_247765955.1"
                     /transl_table=11
                     /translation="MISRLEWHLAQQEDQVRDHGRWDQVQAVASGADPVLSGTPQHELH
                     ELYFSIMSLAARKIWASSGKSTYSIHPGLLAALRTSQSGELPRVIFERLQRHPNPLVIF
                     PQPIPATLPDGAAANVHGFLVHGRLAETRGGLCGSDDPQRRGIGVIVIAGVLDHHGVEV
                     SIDQTQLTLPLVTDHFTIDQIVDQVLTAFAPQPGIPLASERQRDWLILAVRLVLHVLLY
                     LCSEGADIAERSLPTRNSGQNRNTNSTKNRAPKPAKIYQVGATHGPQLLATAGYENLGR
                     GVFRAVAAAHEGELPRLLFHQAHQLDDLLTAALDDLSKMRQQLHITGLARDTLQARVLE
                     LEKQTAAQSADLKKAMATCERWQADAESYRAQAEAVGSPEQLAELRLALEDQAQQLAAA
                     HRQIDELGDQAATYQNERARRRHYSPAVVTGHQSEPAPDSAEELVPATWEALLSTARAL
                     PHLWFSPAIGDTIANLQPRPAWLAAAWNSVRALSHYAQAKHRARVIGDSMTTLRNFSAY
                     LHEHSNGTKISAAQVALSESDAVINNPRLSRVRIHPVPVEIDDTGTCLYLAHVRIGHSP
                     PRPRLHFRDETDNHDVICIAYIGPHLPIT"
     gene            21475..22092
                     /locus_tag="MZC64_RS46065"
                     /old_locus_tag="MZC64_46075"
     CDS             21475..22092
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MZC64_RS46065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MZC64_46075"
                     /product="hypothetical protein"
                     /protein_id="WP_247765954.1"
                     /transl_table=11
                     /translation="MTTTAPYTEQLSTLGAWACSVLLPQTPFDAVRTLLSDLTTYTGTP
                     LNAFLPGDGDLVTELLLVRDPNPTYGTSPDIATCEAFAQLLAAETGWPRVPQRVPSTGV
                     VVGLGLREGNAPDTPQHALAEAHALLAEHGDGWRCDSAHLVSARLVNGSVRWHDELGVI
                     VYADAVLVPVIERIAALFAQDRFVITDFSTGSTRVLTRRITA"
     misc_feature    complement(22122..22136)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.83"
                     /tool="antismash"
ORIGIN
        1 tgctgggcct gccggagttt ccctgggtga ttcctgcggc cgcggtggcc tttgtggtcc
       61 tggtctgcgg agtgagccgg atcgagatca acgcccgcct gctgggaggg ctcctggtcg
      121 tggagtgcct gctggtggcc ggattcgtct tcggcgccgc gctgaccccc gcacagccgg
      181 agtcgatgct gctcgccctg tctcccgatg cggtggccac cgggtccttc accgccgcgc
      241 tgtgcctgtg cgtggctgcc tacaccgggg tggaatcagc gggtctgtac gccgccgaat
      301 gccgtgagcc ctcccgcacg gtcgccaagg ccacctacat cgcgatcgcc gcgatcaccc
      361 tgggctacag cctcatcgcc tggtcgatga ccgcggccac cggcccagga caggtcgtga
      421 cccaggcaca gaaactgggg tccagtttcc tgttcaccgt cgggtcgcaa ctgctcggcc
      481 cttggttcgg acaggtaggg ccgctgatct atctctcctc gatgtttgcc gcgctgctgg
      541 ccttcgccac catggtcgcc cggtacctgt tcgccctggg gcgtgaacgc gtgctgtgga
      601 ccgcgctggg ccgccaacac agcgaaaccg gcgcgccggt ggtcggagcc ttcgtccagt
      661 tcgtcatctc cctgggcgtg gtgatcctgt tctgggcggc tgatctgcac ccggtacagc
      721 acctcgcggt ctggacatcc ggtctggggg cgctggggat cctgctgctg atggccatga
      781 cctcgatcgc ggtgatcagc tatttccggc accggaacac cacggtcagc cggtggtcac
      841 gcagcatcgc cccagccctg tccgctggtg tcctctccgc cctgacgctg ctggtactgc
      901 tcaacttcga catcatcttg ggcagcagca cccccttgtg gtggatccct cccgccgcag
      961 tcatcgccgc cgggctcggc ggcctcggat gggcaagctg gctccaacgc caccgtccgg
     1021 agatctatga ccagatcggc aacggcggcc gccaccacag cgaaggaccc accggcttcc
     1081 tgactcacta agttgccgca gcctacccag cacagagact tctgcgaagg acccccgatg
     1141 accaccgcac agcctgccgc ggtccccgcg ctgcatcccg ccgacccgct caccgcggcc
     1201 gagatcgaca cagtgcggga cctgatcacc agaacgcacc ctgatctcgc cctgcgcttc
     1261 tccctgattg cggctgtgcc accgaccatg caggacaacg ccgccttccg tcgtggacaa
     1321 ggccagggaa cccggtgggc cacggtcatc gccaacgacc accagggcca gacctacctc
     1381 ggagaggtgt gcctgtctcc accgcgcatc tccttgtggc aggtccggcc aggactgcag
     1441 ccggcgatca ccagcgacga actgatgacc ctgtcagaga tcgtgacctc cgatgcccgc
     1501 gccgtcgcgg cgttggcacg ccacggcatc atcgatctgg agttgctgca atgcgatccc
     1561 tggaccgcgg gcaatctccc gatcaaggga gtggacccat ctcggcggat cgtgcgggcc
     1621 accctctacc agcggcactt tccccaagac aacggctacg cccaccccct gcccctgatc
     1681 gtcaccgtcg atctgaccca gcgcaacgtc ctgcacctgg aggacaccgg ccccgcgccg
     1741 attcccgccg actgcgccaa ctacctcacc gaacacctcg ggccaccccg caccgacatc
     1801 cgccccctga ccatcaccca gccggaaggg ccgagcttca ccgtcgacgg cggtgtgatc
     1861 acctggcaca actggcgcgc ccacatcgcc ctgcacccgg tggacggcct ggtcctcagc
     1921 gatgtgtcct atcaggacgg agaccgctcc cgccgggtgc tgcaccacct ggcactggcc
     1981 gaaatggtcg tgccctacgg cgacccccat ccgctgttct cctggcgcaa cgccttcgac
     2041 gtcggtgagt accacctggg acggcaagtc aattcgctcc ggctgggctg tgactgcgtc
     2101 ggcgagatcg tctacctgga tgccaccatc cccgaccatg acggccggcc cgaagccaag
     2161 cccaacgcca tctgcctcca tgaggaggac gccagtatcg cctggcggca caacaacttc
     2221 cgctgggaag accgccccgt tgtccgccga gcccgtcgcc tggtgctctc ctcctggttc
     2281 accgtcggca actatgaata ctgcgtcgaa tactcgctgc actatgacgg ccggatcgag
     2341 tgcactgtca aactaggcgg gatcatccag acccgagcgg cactgcccgg tgaacccgac
     2401 ggctacggcg gccgcatcac cccggagctg atggcgccca ttcatcagca ttttttcgcc
     2461 ttccgtctcg ttccggagat cgacggcgca gagatcactg tccatgaggt agacgccgtg
     2521 gcagccccac cgggacctga caacccggca ggcaatgcct tccggaccca aacgaccctg
     2581 atcgagtccg aggccatgtc gggccgggtg acagatccgg cccgggggcg gatgtggcgc
     2641 gtcagcagcg cgacggcccg caacgcggcc ggcgcgccca ccgcctacag cctccacctc
     2701 aatccggccc ccagactgct cgcagcaccg gagagttcca tagcgcaccg tgccgccttc
     2761 gccaccaagg acctgtggat cacccagcac aggctcagtg agtggtatcc cgccggcgac
     2821 tttgtgaatc agagcaacgg cggtggcggc attcccgact ggatccgtca acaacgttca
     2881 ctgaccgaca ccacgctgac actgtggccg gtactgggga ccttgcactc accgtccact
     2941 gaggactggc cggtcatgcc gacagcccag acagggttcg ccctcacgcc caacggcttc
     3001 ttcgaccgca acccgatgct cgaccttccg gcctccgctg gcacaacccc cagccacgat
     3061 cgctcagcct gccactagca accagccacg atcctcaccg caggggggac actcccgccg
     3121 cagcacagcg ggtggtgcct actgtgcagt caccgcgccg aggatccgcc acgcaaccca
     3181 tgtccgcact gacaggaaat ctatgaacga ccccgatacc caccatgagg accccctcca
     3241 cagcccagga ccattgagcc aggccgaagc agccatggtc atcgacctgg tctgtagcga
     3301 acactccacc gtggaccgct acgccgtgac tgtggaactg gcaacgccag agccaacctg
     3361 catccgcagc ggagccgatc aggaagcggt cgcctcacgc tgggcctcga tcacggcacg
     3421 agacagcacc accggggcgc ggttctcagg cctggtgaac atccgcgaca ggcatatcgc
     3481 cagctggcgg ctgcacccgc cggagactgc gtcacctggc tcagactgac ctctgccgct
     3541 ggagctcagg cgggccgtca cagcagccaa gcacaacggc tcacgatcgc gcactgctgc
     3601 tgacctgacg ccaaacaatg tccccgatcg ttcaccggcg acaccacatc ggcgtccaca
     3661 taggacacca acgacactct cggaaccaga agggcacagt gaaccatggc cgcagccatc
     3721 ggcaccgaaa tacgacgcgg gacccctggc gaccaacggt ggccgtacgc ccctggcgtt
     3781 agtcgagcac gtctgctcaa cggctgcctc ggcggagcgc tcactacccc cgcagaccgc
     3841 acactcgcca cagcactcga tgccgcggac ccgacctggg gagcagccgc cgcgatcacg
     3901 gcgacgtttg tcgaggagac actcctcgcc ggtgccaggg cgggcatccg acaggtcctg
     3961 cagatcggca acggtgcgcc cgtacctacc acgatgccgc acgaacagtt cgccgcacac
     4021 caccgcggca gcacgaactc catcagatac atctacgcgc tggaggatcc ggtcgaggcc
     4081 gccctagtgt gcgaggaact cattgccagc accagagagc ccgctccagt ccccatggat
     4141 gcccgggtag tacacgtggc cgactctcgg ctcacgacga tcctggccga tgtgcgggcc
     4201 tgtcaggcat tgggtgacca ggaaccgctg ctgatgctct tgcccctgac cggcctgaac
     4261 gacgccgagt tccgagagct gatcacccac gcccgcacag ccctgccagc gggcagcgtg
     4321 atcgccatcg ccgccaccgg attcggctgc accccgacag cgaaggagat cctggtagcc
     4381 aagaacatgg gcaggatcta tcgcgacgct ggcctctcct tcccgctgac cacctgggca
     4441 gaactggagg tggcgctgcg ggactggcgt ctgctggaac cgggaatcgt ggcccagaca
     4501 cgctggcgcc accaaggctc actccctcca gcggccatac cggtcctcct ggccatcgcc
     4561 acagacccga gggcagacat ttccgcggtc tggcaacgcc cctctcggcc gaggcaggca
     4621 tcgctgcgcc tggtcaaccc gaaccggcgc agcgcaccgt gaccaccact gtctacagtg
     4681 gagtcaggaa acaacttcag atcgtgggcc tctgagacgg acatgaccac actccctcga
     4741 tcatccatgc ctgcccgagc ggaccgtcga cacagaatca ccgcgctgat gctccgcggt
     4801 gcgctcagcg caagcggcac gaccgcgatt caatcaccct cgggtaccgg agtgcaccag
     4861 cccaaggctc tgccgcacaa gcaccacgga ctgatctgca cccagtcacc cgatcctgac
     4921 aggttccctg gcatcaccac agggatcacg ccctccaaca cagaaaggcc cgaccggtga
     4981 tcgccgtcag cacccgagca cctcaactgc ccgtgcgccg cagtcgcccg tggacacatc
     5041 accagatgtc ccagacgccc gaagccaggg gaagtggaca ccggatggtg aatgaggccc
     5101 gggggcttcg cccacgcagg cgccaccggc ctggagccca tgaccatcgc taccgggtgc
     5161 gcgaggccag tcaccgcaga cgccaccgcc gggccacttc ccacctgtca caggtgcgtc
     5221 cgcatcagta ccgcggcggc accagagcgg aaggacctcc acttcacagg acgcacggga
     5281 agcacgcgtc ccggccgaac cccacagcgc tgcacccgat cagccagccc gcacgcagca
     5341 ccagcgatca ccgccgccca acttcccttc ccactgagcc actccagacc cagccgacag
     5401 actcatgacc acctccactc ctgcccaaca gaacaaccag ccgacgcgca cacgactccg
     5461 cccatcgcag tcaggacaca ccagtgcgat ctgtcagagg gcggcactgc ctacctgcgc
     5521 actgctcctc gcaagggcag ggcacactcg gcacgctgtg ccgacaagct ccgcatcgcc
     5581 gcggccagtg cccgcagaca tgtccgagga cgcggctcac tgggcactgc acctcgtcga
     5641 tgcctgcggc ctggcccgct ccgccacctg cttctggacc gtgccgggca acgtcgagtc
     5701 cgccgcaccc cacagatgcg acacatgcct ggtcccaggc gctgcggacc tggccgtctg
     5761 gggttgccgc cgcagtgact gcccgcagcc gccacaccgt agcgggtgca ccgcccgtgg
     5821 acacccgagc atgacacctc tgcctttccg acacccactc accaccacgc cggaagagga
     5881 gccatggccg gctcgttgcc actgcccatc cagtcccata gccaggcgcc cggaaatgcc
     5941 gagcacaccg cggtacctga tacggagtgg tgccgagaac agttggaccg ttccctcctc
     6001 cggtaccgcc gggcactccg gaccggggca ccgacctgcc ttctcacggc cctgatcctg
     6061 ctgggattcg gcacgaggat ccaccgcgag gtgcacaaga cggtgggcgg cctcatggct
     6121 gtgaccggtc tcgtcgtgat cgcgatctgg ctcgggtggt ggtggctgcg ctggatggtg
     6181 ctcagcaccg accgatcgat gtatcgactc agcgtcgcag ccaatgaagc cgtggcctgg
     6241 tccggcctgg aaccgcgaca ccttcgccaa ggcctgatgt acacccgaga acgcggtggc
     6301 gtgtatcagt tctacggccc actgcccgat cacgcgctcc acgccgagag cagcagcacc
     6361 caggacggat agcgcctgcc tgcctcggca accggcgcac accccgcaag cccggcgagt
     6421 gccggtactc ccactgttga tgtttctcct caccgttgga caccaatgac gatcaccacc
     6481 ggccccagtg cgattcagga tgcagcccca gcagtccacg gccacgcgaa gcgggaggct
     6541 gtcctgcgat gggcgcggat actggaccag gccaagccag cctcggtcat accggaacca
     6601 cggcagcgac catcacagtt cccgaccgcg ccgcaaccac tgctcggcag ggaccgcgac
     6661 ttggctctcc tggacgagct ggccgccctc caccgcgcgc aaccgacgtc tgcgaccgtg
     6721 gtgatctccg gtgaagtcgg gataggaaag acagccttgg ccctcacctg gggccagcgc
     6781 aaccagcagc agttcccaca cggggcaatc tggctcgatt tggccgacta ccgacacggc
     6841 acccacgctg ccttggatgc cactgccgtc ctggaggcac tgctgcgtgc actcaccatc
     6901 ccggcctggc gaatcgacgc tgcgcacacc agagcactca aggtcgagct gctccacaac
     6961 gccaccgccg gacaacatct actcatcgtg gtcgacaacg ccgaagccac cgacgacctg
     7021 ctcggactgc tgccgagctc gacaacctgc gtcgtcctga tcaccactgc tgtcggcccg
     7081 ccaggactgt ggggacttcg tgaggccaag caccacgtcc tgaccccgct ggatgacacc
     7141 gccgcgacgg aactggccgc cggcattctg ggcgcctcac gaatcgaggc cgcggaccaa
     7201 gcgtgggagc agatcctgac gcactgcgcg gggaacccgc tggcattgcg actagccgcc
     7261 gtcaccatcg ccacccgccg gccatccagc ctgacctggg ccgcacgccg cctcaccgga
     7321 gaaaccggga cctgccgtgc caccctcggc acggcccgga gtaccgatgt ggtggcatcg
     7381 gcgacagccc tggccctgca gggcgctggc ccactcttgg tccgccgggt gcgcagactg
     7441 gcggagtatc ccggtcacga gctcactcca ccggtagccg cagccgtcct cggctgcggc
     7501 accagtaccg aaggaggaga gatcctccaa cgactcaccg accgcggcct actgaccatg
     7561 attcatccga accactaccg gatacctcgc gccctggcac gcaccattcg ccacgccaca
     7621 cctccactca gcgcggcgtc ccaagccgca gcagtcgccg ccggccttcg tcaactggcc
     7681 cgcacagccg tcgccgcgat tgacgtccta ggtggatcgg gcgacagttc gctgcgaact
     7741 ccagtccgtg atggctccac cgcggccgac ctcttcgaca gccctttgca agccctggcg
     7801 tggctggagg ttcaccgcag ggacctccag cgagccgtca ccatcaccgt gcagctccgc
     7861 gagctccgat ccatcgtctg gccgttgcac tacgcactga gccggttgtg cgccctccag
     7921 ggcggagcgg ccgactggat ccggatctat cagaaagtcc tgcccctggc gtgcgaatca
     7981 ggcatgcagc gaaggcaaag cgaggttcgc ttcggcctag cgatggcacg cctcgtggcg
     8041 ccgcggccct atcgtgccgg atctgcggac gcaggcttgc cagcctctgc acacctggac
     8101 ctcgccagcc gtcttgcctc catcaccggg tcgagattcc tcagcttgcc cgagcagtgg
     8161 aacggttttc tgccctatct cagcagccgc tgggccgacc cacgagtagc cgagaaactt
     8221 cactgcaccg cagccgcgat cttcgtccgt gaccacaacg cgcggatggc acatgtgcac
     8281 aacatcggac tagccgaaag ctgcgctgcc acaggccgat ccgcgcaagc actggcactg
     8341 ctcaacaccg catcgcaggc attcgcgctc atggacgatc gcgccgcaca ggtgcgggtg
     8401 ctgatcacca tggcggctgt tcatctggga ctcggtaaag cccacaacgc tgcagatgcc
     8461 ctcgcccgcg cccgcatgct cctcagcccg gaatcctcca gggcagaaca cgctgcggtg
     8521 gaaggcagta cagccatcgt ggctgacacc atggggcagc acagccgagt ccgcagtgcg
     8581 cgtgcagcgc tggcacaact gctcccggcc agtggctcaa cgcccgcgat caccgctgtg
     8641 ctggaaaccc tggaacgaga aggaaaccgc acagcggcga gtgctaggca gaacacaggc
     8701 cctgtgccac acgcagtagc ggcacggcac gatcgacctc caggtgtctc tcaacaacac
     8761 cacacctcga ctgtcctggc ctgaccttga gttctatacc gctagccatg aggtggactt
     8821 acaccgtggc ggcgccttcc gcctgcatcg gccgccccgt acggctccgc acgccctgtt
     8881 gttgcgcggc cgcattcgcc acgaccgttg ccccggccgt ggggcctggc agatgtttgg
     8941 cgccactgcg gcgatgtccc agccttctca cggatctgct ctgccgtgcg tcggtggcct
     9001 cggcgacagg ggagcagtgc tggcacccca caaccaccat ctccgatcgc gtaccaggag
     9061 aacagtgtga cgacgctccg accaacctcg cttcgctggg ccaccctgct ggggtctctg
     9121 ctcgcgatga tcgccttgac cccgctgatc ctctactgga ccggtgtcgc tgcgatgggc
     9181 gtggccagct gctcgatgac caccgacccc gcgcaccagg ggatctgttc ggtccccatg
     9241 cagttcctcc ttctcatcct gccctccgcg gtctgggtgc tgtctttcgt ggtcatgctg
     9301 gtgtgcggga tcgtcgcgat cagccacagg cggcggctga catggtggat cgccggtcct
     9361 gcactggcca caggctttgc cctgggggtc agtgtcggcc tcggtctgtg gctgggctag
     9421 ccacgctgac ccgttctggt gacacagcca tgcgacaggc cgccgatgtt cgagcaggtc
     9481 tgatgttcca gcctcgacaa cggatgtggc tgcacaccaa gcgtccacga cctcagcgtc
     9541 gcggacaggg cgcacgcgaa ccaggtggcg cgctcacgcc ggcagcggga ctcgctggcc
     9601 agggtaggcg cggtactcat tgcggcactt cacacatccc attgcctgac tgcaccccat
     9661 catccccgga caccgcacag cggtcccaga tgagtccatg cttgagccag gcgcaaccac
     9721 acactcaccc caccggtcgt ctcgtacgtc tcggcacggc ggcgcacctt cgccggagtg
     9781 cttgcgcagc aggatgtttc gttgcacgat ggggatgggt gagtgcacgg aaaggatggc
     9841 catggaggcc actacgttcc cgaccgatgc aatcaaagaa gcgccgcgat atgagccgcc
     9901 ggaggtcatc gcgctcggcg gtgttgtcga gctgacccag ggcagtgcgt tcgacgacac
     9961 catggagaaa cggtcctact atttctaggc ggatcacgtg atgtggttgg cggaaaccgt
    10021 cggccgcgac gctctccacg tacctccagc acgcgcgtgc ccggatctga ctgccgtcgc
    10081 cgccgactgg attcggttgt ggcacaccag atccgggcca cccggcggcc tacggaaggt
    10141 tgacgttcca ggctgtcgcc tgctggtgct gggctactgc cccgcggatg atcgggagct
    10201 gaccaccatc ggcgagcagg tggctcgcgg cggtgattcg gcactagcct cggtcggcgg
    10261 gagcagcgtg gtgatcgccg tgcggcgcga tgatgcagtc atcgtgggag atctcgccgg
    10321 acaacgcacg gtcttctaca cccgcactcc caacgggcgg accgtactcg gatctcactg
    10381 cagtcaccta gccagaactg tcagcaccgg agtcgacaag ggatggctgg cgacccgctt
    10441 gatccttccc cgcgcctcgg acgtgtgggc cgagggctca ccgtggacag gagtgcaggc
    10501 actgcggcca ggctgggggc tgagactgcg ccgcaccgga gccagctccg tacaccaagt
    10561 gcacaccttg cccactccag agacagaaca gggggacgcc gcagtcgttc tcagaacagc
    10621 cctgcgtcag gcggtagaac tccgggtgcg cacagcccag gtcagttcag tcgacctgag
    10681 cggcggactt gattcctcca ccgtggcaac cctcgcggcc aagacagcac ctggccgagt
    10741 gcctgcgtag acgatgacca ttcccgacgt cgacgatgct caagcagccg cctcggtcgt
    10801 ggctcaggtt cccggcctga cacaccgaca acgtcccatg ccaccgcatg tcctgcccta
    10861 ttccgaactt gaccagctac ccccgctgga cgagcccggg gccctggctg catccagtgc
    10921 ctgcgaacgg tggtggcatt cccacatcgc cagcctcgga ccagacgtac acctcggggg
    10981 cgatggtggg gacgcggtga tgaccgcccc cctgctctat ctcgccgacc tggcccacct
    11041 acgcgcggcc gggcaactct ggcggcatgc ggtgggctgg gcacagttgc gaaaccaagc
    11101 accccacctg ctgatccgca ccgcacggtc ggcgcggcag gcgaattacc gaagcgaact
    11161 tggtgcgctg gccgcgcagc tccgcgccgg cagcctcccg gcggccaggc cgatggcggc
    11221 actctcgctg ttgccgcaca ccgcggtcgc ggcctggatg agcagacagg ggcgagagct
    11281 cgtggctgag cgactgcacc agcacgccgc agagcacgcg gagccggtag ttcccggcag
    11341 cttcggcatc ggtgacacgg ctgcctggct caccctgacc gccttcggca ggcaacaacg
    11401 actcgatgcc gatctcgccg cgacccaggg aatcaaccac cagtcgccgt ttctggatga
    11461 tgcggtcatt cgggcctgct ggtcgatacc ggcctggcat cgcacgactc cacaccaggc
    11521 gaaaccgtta ctggtcaacg ccttcaccaa cctggtgccc gcatccgtgc tgcgccgcag
    11581 caccaaaggc gaccacacgg cttatgccta ccgcggtctg gctaccaacg cacccgagct
    11641 gacccgcctg ttcaccgact cctgcctcgc ccaggttggc ctgatcgagg actctgcggt
    11701 ccgcgcggtg atcagccggg cccgggcggg gcaggcaatc cccctcggtg ccttggacct
    11761 gctggtcacg accgagttgt ggctacgagg caacagcggc acacacgact cggcaggaag
    11821 ggacacgtca tgacggtgcg agcagcaccc ggtgtacacg ctgtcatgat cgagggggga
    11881 accatgatcg tgctggccga acgctccggc cagtactacc agggctcttc cacagcgcgt
    11941 gccatgtggt gtgccctgca gacacacgct ggcgacctgt ccccggcggc gcagtccgtg
    12001 gcgacggagt tcgatgtgcc ggtcagccag gtacgagccg acctcgaaac cttcgtcggc
    12061 cgattactcg aactctcgtt gctggaggtg accgcgtgag catgcccatg gcaatgcggc
    12121 agaaacggcc accagggttg ttggccacca gcatcggcgc gtgcgcgggc ctggcggcct
    12181 gggccgtgct tcgcctgatc cgattcgatc gtctcaccgc cgcggccgag gctctggcgc
    12241 ggaccaccca gcgcgccgcc acagccgagg aaactcagcg cgcgctcgac atggtcaccg
    12301 ccggtgcgcg ctggataccg ggacgggtcg cctgcctgga acgctcgctg gcggcgttcg
    12361 tgatcctggc ggtgtgtcgg cggggcgtga gctggtgcat cggagtacgc acaccaccga
    12421 tcactgcgca cgcctggctc gccgacgatg ccggcacacc gctgggcgaa cctgactcca
    12481 caacaggctg cagaccgctg cgggtcattt ctcgcctgcc aggaaccacc agaggaagct
    12541 ggagaacatc gtgaacggca tcgatgtcga ctaccaggtg tgggttcaca cctccgaggg
    12601 ggaagtcatc ccacaaagca cggcgccggc cacgatcgag cggatgctcg gactcctggc
    12661 ccccgcaccc ggggccgcgg tgctggagat cggcaccggc tcaggctata ccggcgccgt
    12721 gctgtcccac ctcgtcggtg acagcggccg ggtggtgtcg ctggatgtgg atgcggactt
    12781 ggtgatccgg gcccgcggcc tgcacgcctc ggccaaccac cgcaacatcg aggtgcatgt
    12841 ccacaatgga ttccaaggct gggcgccggg agccccctac gacgctgtga tcgggtggac
    12901 cacggctccc gtcctgcccc gagcgtgggt ggagcaagcc gctcccaccg ccgtcctggt
    12961 cgcaccagtc aagattgccg agatcgccca cgccaacgcg gtcattctgt gcaccctcca
    13021 ccagggtgcg ccggtcaacc ccgcactgca cccaggatcc ttcatcgaca tgcaccatga
    13081 ggtcatcgag gaactgggcc gtcctgtccg attcgtcgac agcgaactcc gcgatgacgc
    13141 cggatcggca tggtggctca gcgccaccgc actccgagac cacgcgatgc gagagtcggc
    13201 gacagcgttg ctggaacaga tccggcttgc cgagcccgtc acaggcttcc tcccgcagga
    13261 ccggcagacc atggagtctt tcgccgcctt tgttctgagc accactacga gtcccggcag
    13321 caccgggggc cccggactgg gccggtgcat cggggtcgtg cacgcccagg gtgctgcgtt
    13381 cgtcacctct catggccatc tgctcgccgc cggaggcacc gaggcagtcg aggaactggg
    13441 ggagctgcgc cggcagtggc agcaccgggg ccggccccgc ctccaggatc tagaggtggt
    13501 gctgaccgag gtcgccgagg gctgggcggt acgagcgcag tgtggtgcct aggccgccag
    13561 gggagcggcg gggtcagcga ccctgatggt cctgaccccg ccctcctgga ccgggcgcgg
    13621 tggggtgagt gcacacctgg tgtgtgaaca accgcagccc ggaggaaaag ggggcgcagg
    13681 tccgcgctcg gcctgcgccc gggaatgctc gacgggcgcg cacactgcgg cagggcctgg
    13741 ccgcggtcgc gcgtcgagct cagtcctggg cgctcacccg cagcagcgcg cgaggccggg
    13801 ccccgatgat ctccagcacg ctgtccttct cccgcggcgc atgcagtgcc tgcgcgaccg
    13861 cgcgcagagt tccggagccg ctccgcacca cagtcggttc acgacacggg acacaggggg
    13921 agaaggcggt gagaacgcgg actcccacta cacacagaga tcctccccac accgcgtcac
    13981 tgtccaggtg atgtccagtc ggatgcacca gcaacagacc ggtcacccga ccatgccagc
    14041 ccggcgtcgc ggcaacactg aaaccccatc cgctgatcat ggcgccgatg gtcaccccgt
    14101 ggacggccag ctcacctccg cccctcgggg cagcaggcaa ccctctgaac cgtggcctct
    14161 gcagtgatgt gtccgccatc gccctctcct tcacatcagc acacaccggg gcggttcagt
    14221 cggtgaacac agcggtgacg gcgcgaacga cgacacacgg ccacaccggg cgtccccgcc
    14281 aggacctcgg gcatccgcga caccggccgg acttgccggg ctggtggagg tcgctgagcc
    14341 agcgccaccc cgtgatcagg cggacgagcg cggcgtactc ctcccggccg atcctcccag
    14401 tctccagcag cccgatcagc acggcagtgc cacaggcctc ctccgcacac accacttcac
    14461 gcgcacgcgc cttctccggc tccgcagacc gcattcgggc ctggggcgca gcgacaccgc
    14521 cggcggtcat cgtgggctgc ccagcatggc catctcgggc acatccgcgc ggcccggagc
    14581 gcccagccac tgcgcgagaa cagacaacag atcaccgagc gtcgagatcg ctgccgccgt
    14641 cggccgaagc tgacccacag gggcgaccgg ctcaccgtcc ccctcgcggc ggcagtgtcg
    14701 ctgtgccaca ccggcacacc cgcactcgct gtcctcagcg accgccgcaa gaagaaattc
    14761 accgccgcgg cggtgagaaa gaaccgtcct ggacatcctc tggcctccat gggtgggtgc
    14821 cgcttggcct ctccacctgc actcaaccgg cccgaaccgc gcggcgacag agccttgatc
    14881 cggacatttc tcggacatcg acaagacatc gcagcctctg aacaggcaag atgcaagtac
    14941 tcgtgacacg cgcagtcaag cgggggagag acagtcagat gggcgacagg ctggccctga
    15001 gacaacgccg ggaggcatgc ggctacagcc aggaacagct cgccggcgaa gtcggtgtga
    15061 cacccaggac cgtcggccgc tgggaaagag ggcagagcaa tccccagccc tggcatcgac
    15121 cgcggctggc cgcgctacta cgcctgggca tcgagcagtt aggggtcctg ctcactgaga
    15181 acgaaccggg tctgacaggc cgagacctcg cgtcagggac acacacggcc ctgccagaga
    15241 ccctcagtta cggtcacacg gtcatcatca gggggcagcg cgagacaacg gagtgcgacg
    15301 acgtgaaccg ccgagacctt ctgcggctct tcagcttcgc cggtgcagga ctggcattcc
    15361 cagcagctgc cgatgccgtg gattgggacc gactcgccta ccattccacc cacgcgctcc
    15421 ggcctgaccc agagacactc gaccagtatg agaagctcaa ccttcgcttg tggcgagaat
    15481 acagcgatgc tgtctcgaaa atggacatgc tcgcagtggt tcgccaccag ctcaacgagc
    15541 tgaccagcag cctccagaac agcccagagg gaccatccag gcggcgcctg tccgcataca
    15601 ccgcggacct gtttcagctc gcaggtgaaa tcctcttcga cgccaacagg tacaccgatg
    15661 ccgcccagtg ctattcgttg gccactgagg ccggcaagca ggcgcaggac tacgatctct
    15721 gggcctgtgc cctggtccgg cattccttca tcgacctcta cgaccgccac ttcggcaagg
    15781 ccctgccact gcttgacgcg gcgatgaggt gcgcgcagcg cggagacccc agtctgccga
    15841 cacgccactg ggtcagcgtc gttcgcggcc aggccctggc cgggctcggc caggagagag
    15901 catgccgtca ggcgttcgac atcgctgacg gcgtccacga catcgatggg cggcaccgca
    15961 cctcaggatg gctgcgtttc aacggaacac gcctccctga ggagcgtgga gccagcttgg
    16021 ttgagctggg tatccctgcc ctggcagaag cgtcattgac cgaagccttg cgaggtccgc
    16081 tgtccacacg ccgacgaggc accgtgctcg ccgacctcgc tacggtcggc ttcctgagca
    16141 gagatctcga ccagatggcg ctgtcggctc ggcatgccct ggaagccgcg cagcagacag
    16201 gttcaggtgt catcgaacgc aaactgctcg ggctgcggga gcgctttatc cctctcctgg
    16261 gagagaagcg gatcagcgtg ctagacaatc agattaccca cctctttccc acaggcagta
    16321 ctcatcgtta gtgaggagcg tgacttgcag cacgaacctg gagaattttt ccggcagacc
    16381 tggattgacg cagtgaatga gcactatccg ggcgagccga aaagctccta tgtggctccc
    16441 tggcccgaga ctcgagagtg ggaacaacgg tgtgctgccg cggtggaagg gcagctccga
    16501 gattttctga ccagatccga cgggcatgcc gcccaactga cccaggaaca gaaaggccag
    16561 tatgtcacct tgtgctggct agcccaggtc taccggcacg ttgacgaccc gaaaccggcc
    16621 tatgtggccg gttggggaga catgcaggag tggcatcgtc gagtgaacat cagcatcttc
    16681 ctgcgattcg aacaagagtt tcaggcttcc tagaccggga ggacgaggca gccccacatc
    16741 ggcagccggg gcaccggagc agcagtcgcg tgctgtgcca cagacaccaa cgcgactgcc
    16801 gacggcaggt acagaacagc agtcaaagcc ccagtagcga ccgaggttcc cgactgccag
    16861 cactggtgac tcgtactcac accggatccg cccctcgggg catgaccgag cacgtcacgc
    16921 cccgaggagt cgggaccgac ccaacccgcc cgctagtgcc cctcccgcga gataggcgag
    16981 gtggtctgta ctccacctgg cactgcaacg gagtccgaca ctgagggagc gccacggcca
    17041 ctccgccaca gccgcagaca tccaccgccg caccgcgctt gtcctcgtgt catcgggatc
    17101 ctcgagcagc tcaggggagc gccagcgccc cgtctgcttc agcggccgcc gctggaagca
    17161 gacacctggc aacgacacgg gttgctcgcc ttccgtggct ctgtggcggc aggaaatcgg
    17221 tgcaaggtcg cagagcgttc cagaactggc ggcatcggcg ataccagggt cctgttcgat
    17281 cctgagattt cgtgataccg caacgtatct gcgcagactc atccgttatg gacagtgatg
    17341 cctgttctgc gccaacaaca ttgtgtaccg caccgcctcc gtgcgctgtt cgcggcgtgc
    17401 tggaaccctt ggcccgcacc gtgtaaggtc cacgatggag tgatcatcgc gacgatcaca
    17461 ccggcgggcc gggggagacc ccttgcgatc ccgagccacc gagcccttat ctgagaacgg
    17521 ttcccggccc gcactgccgc tgacaaccaa ggatccctgt gccacgctgc ttcgctggaa
    17581 ttgactggtc cgaagggctc aatgacgtcg ccgtcgtcga cgactccggc gagatcatcg
    17641 cctcggcgcg catccccgaa actcccgagg ggatcacgga atgcttgcgc ctactcgcca
    17701 aggccaaccc cagctcgcga cacaggcgcc gtgacgtccc catcgcgatc gagaccggga
    17761 cgggcctgct cgtcgacgcc ctcgtgagcc gtggccaaac tgtctatgcc atcaaccccg
    17821 ctgtcgtggc ccgctaccgc ggacgcctgc accccaccag acagaaaagc gaccgcagag
    17881 acgcggccct gctggccaac attttgcgca cggacctcat ccaccatcga ccgctaccag
    17941 tcaacacccc cgcggccaca gcgatcgcgg tcctggtccg cgagtaccag agcgcggcgc
    18001 gtgcacgtca acgtcacttc aaccggctcc gatcccaact gcgggagtac ttcccggccg
    18061 cgatcaccgc ctggcaggaa ctccccaacg gcctgctccg cccggaagcc cgtgcgctac
    18121 tggcgatcgc gcccactcct gccctggcgg cccggcttag ccggacccga gtgcgcacga
    18181 ccctggccga ggccggccgc acccgcctgc tcgacgatca ggccgatcgg ctgttcgacc
    18241 acttcagaat ccgccaactc cgcacccacc ccgtagtcga ggaaaccatg ggcatccgca
    18301 cactcgcaca gctggacgcc ctcaaccacg cagaccacac cgcaaccgaa ctcgctgaac
    18361 acgcaggcac cgccttcgag gcacaccccc actccgcgat ctaccgatca ttcccaggcg
    18421 tgggcccact catcgccctc cgcctcttcg ccgaactcgg agacgacaac acacgattcg
    18481 ccacagcccg aggacttcgc gcctacgccg gcgcggcacc actgacctgg gccagcggca
    18541 gcggcagctc ggtgtcctat cgcaggatcg ccaacaaata cctcatggcc accgggcacg
    18601 tctgggcgtt tacctccctc accaaatcgc cgggctgccg cgaccactac gaccgccgcc
    18661 gcgccgccgg cgatgcgcac gccgcggctc tgcgtaacct gttcggccgc ctcatcacct
    18721 gcctgcacca ctgcctcaac cacaacgagc tctacaacga ggccacggcg tttgccacag
    18781 ccgtttctga ggctgcctga cgcctgggct ggaggacagc cgggaacaca cctaggacca
    18841 catcccgggg cttgacccct gacgtgggag cggtgcccgg ctgtccccct gcccacaccc
    18901 caaggaacct ggcgactccg ccacctcgcc tcctctctga gccgtgggca ctgcctcgtt
    18961 gaccactgtg gacagtggtc cgcggtttca gcggtgaccg ccatcctcca tggcgtgccg
    19021 aaatccccaa cagcacaaca gacacacggg gcctggcccc gctctggaac cgggtcacga
    19081 ccatgcggcc tgcgacggtc acccgatcgt ggctgccagc gcggccgata gtgtcctgat
    19141 tgtcatagtg atcatgcggc gcgagaggat tccctggggt gcaggcgaaa tctgtgctcg
    19201 gtcttggtga gcaggctcca ctcttgatct cgcggctcga gtggcacctg gcccagcaag
    19261 aggatcaggt ccgtgaccat ggccggtggg accaggtgca agcggtggcc agcggagcgg
    19321 accccgtact ctcaggcacg ccgcagcatg agctgcacga actgtacttc agcatcatgt
    19381 cgctcgcggc acgcaagatc tgggcctcct caggcaagtc cacatactcg atccaccccg
    19441 ggttgctggc agcgcttcgg accagccaat ccggcgagct gcccagagtc atcttcgagc
    19501 ggctacagcg acaccccaac ccgctggtga tctttcccca gccgattccg gccacgctgc
    19561 ctgacggcgc ggcggctaac gtgcacgggt tcctggtgca cggacggttg gccgagacca
    19621 gaggcggctt gtgtggcagc gacgacccgc agcgccgggg aatcggagtg atcgtgatcg
    19681 ccggagtcct cgatcaccac ggcgtcgaag tcagcattga tcaaacccag ctcacgctcc
    19741 cactggtgac cgatcacttc accatcgacc agatcgtcga tcaggtactc accgccttcg
    19801 cgccccagcc ggggatcccg ctggcgtcgg aacggcagcg agactggctc atcctggcgg
    19861 tccggctggt tctgcacgtc ctgctgtact tgtgcagcga gggtgccgac atcgctgagc
    19921 ggtcgctgcc caccagaaac agcgggcaga ataggaacac gaacagcacc aagaaccgag
    19981 cacccaagcc cgcgaagatc taccaggtcg gcgcaaccca cggaccgcaa ctgctggcca
    20041 cggccggata cgaaaacctc ggccgaggag tgttccgtgc agtcgctgcc gcgcacgagg
    20101 gcgagctgcc ccgattgctg ttccaccagg cccaccaact cgacgacctg ctcaccgcgg
    20161 cactggatga cctgagcaag atgcgacagc agctccacat cacggggctg gcccgagaca
    20221 cgctgcaggc acgggttctc gagctggaga aacagacagc tgcacagtcc gcggacttga
    20281 agaaggccat ggcaacgtgc gaaaggtggc aggccgacgc agagagctac cgtgcccagg
    20341 cggaagctgt cggtagcccg gagcagcttg ccgagctacg gctggcactg gaggatcaag
    20401 cacagcaact cgccgccgct cataggcaga ttgacgagct tggcgaccaa gccgccactt
    20461 accagaacga acgtgctcgc cgccggcact acagtccggc cgtggtgact ggccaccaga
    20521 gcgagcctgc cccggactcc gcagaagagc tggtgcccgc aacgtgggag gccttgctaa
    20581 gcacggcccg cgcactgcca cacctgtggt tcagccccgc cattggtgac accatcgcca
    20641 acctgcagcc ccgtcccgcc tggctcgccg cagcatggaa ctctgtacgc gcgctgtcgc
    20701 actatgccca ggccaaacac cgcgcccgag taatcggcga ttcgatgacc accttgcgca
    20761 acttctcggc ctacctgcat gagcactcca acggaaccaa gatctccgcc gcgcaggtcg
    20821 cgctcagcga aagcgacgcc gtgatcaaca atccgcggct gagcagggtc cggatccacc
    20881 ctgtgccggt cgagatcgac gacaccggca cgtgcctgta tctggctcat gttcgtattg
    20941 gccattcccc gcccaggcca cgactgcatt tccgcgatga gactgacaat catgacgtca
    21001 tctgcattgc ctacatcgga cctcatttgc cgatcacgtg acggctgatc cacctgtttg
    21061 ccttgcaagg tctggcaatg cgcagcgctc tcactttcaa ttctacagct tttcggcacc
    21121 tgtgcagcat gaatgagact cggccgtccg gatgaaggcc aggtgctgcg ctcgtccgtt
    21181 gagctgaccc agccggcgcg aggacgttca cctcggtcac gtgcctgact gctcgcgtga
    21241 gcctctcacg gggcagacga cctcacctac caggtctggc acgatgcctt cgtcatgcac
    21301 cagtccggta ccaagagctt gagagcgcgc atgagcggag agcggggaac atctcccatg
    21361 gggtcgcggc tgggaagctc tgctgtgacc agccaactca ggggcgtaga tctactcgcc
    21421 gcgctcaatc tgcaccgacc cgaccaccag tactgctcga accgaagggc cgtcgtgacc
    21481 accaccgccc cctacaccga acagctctcc acgcttggtg cctgggcttg ctcggtcctg
    21541 ctgccgcaga cgccattcga tgcggtccgt accttgctga gcgacctgac cacctacacc
    21601 ggtacgccac tgaacgcctt cctgcccggc gacggtgacc tggtcaccga gttgctgctc
    21661 gtgcgggacc cgaacccgac ctacggcacc tctccggaca tcgccacctg cgaagcgttc
    21721 gcacagctcc tggcggcaga gaccggctgg ccacgcgtgc cgcaacgcgt gcccagcact
    21781 ggagttgtcg tcggcctcgg gctgcgggag ggcaacgcgc cggatacgcc gcagcacgcg
    21841 ctcgccgagg cccatgcgct actggccgag cacggtgacg gctggcggtg cgactccgcc
    21901 catctggtgt ccgctcgcct ggttaacgga tcggtccgct ggcatgacga gctcggtgtg
    21961 atcgtctacg ccgatgccgt gctggtgccg gtcatcgagc ggatcgccgc gctgtttgca
    22021 caggaccgct tcgtgatcac cgatttcagt actggttcca cccgcgtcct cacccggcga
    22081 atcactgcat agctgagacg tggtcatcgc gcagacgacc gtggccgacg gcaacgagac
    22141 atctcctcgt gagcctccgc gctgctggtc gagcccagca gcgccacgat cacctgcgga
    22201 gcagacaccg acgcccgcgg gcagctagtg tcctgcgcct gaaatccgct ggcaatatcg
    22261 tgcgagtgag tcgatgatct cttcggcggt cttctgccac
//
