LOCUS       NZ_CP095550            11731 bp    DNA     circular CON 11-MAY-2024
DEFINITION  Metabacillus endolithicus strain KCTC 33579 chromosome.
ACCESSION   NZ_CP095550
VERSION     NZ_CP095550.1
KEYWORDS    RefSeq.
SOURCE      Metabacillus endolithicus
  ORGANISM  Metabacillus endolithicus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Metabacillus.
REFERENCE   1  (bases 1 to 5052055)
  AUTHORS   Lee,K. and Son,H.
  TITLE     Metabacillus endolithicus type strain genome analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5052055)
  AUTHORS   Lee,K. and Son,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2022) Bio Health Science, Changwon National
            University, Changwondaero, Changwon, Gyeongnam 51140, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Miniasm v. 0.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 69.5x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023078335.1-RS_2024_05_11
            Annotation Date                   :: 05/11/2024 05:30:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,322
            Genes (coding)                    :: 4,799
            CDSs (with protein)               :: 4,799
            Genes (RNA)                       :: 199
            rRNAs                             :: 16, 15, 15 (5S, 16S, 23S)
            complete rRNAs                    :: 16, 15, 15 (5S, 16S, 23S)
            tRNAs                             :: 146
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 524
            CDSs (without protein)            :: 523
            Pseudo Genes (ambiguous residues) :: 0 of 524
            Pseudo Genes (frameshifted)       :: 474 of 524
            Pseudo Genes (incomplete)         :: 58 of 524
            Pseudo Genes (internal stop)      :: 17 of 524
            Pseudo Genes (multiple problems)  :: 23 of 524
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:11:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 292285
            Orig. end                         :: 304016
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095550.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11731
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11731
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11731
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6731](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6731)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    246..260
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.79"
                     /tool="antismash"
     misc_feature    complement(1221..1230)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 16.22"
                     /tool="antismash"
     gene            complement(1301..2866)
                     /locus_tag="MVE64_RS01570"
                     /old_locus_tag="MVE64_01570"
     CDS             complement(1301..2866)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00881"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252868.1"
                     /locus_tag="MVE64_RS01570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MVE64_01570"
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="WP_247343111.1"
                     /sec_met_domain="PF00881 (E-value: 2.5e-06, bitscore: 25.9,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MILDEFLHNLHFDIDKASIPNWEVDWEDGPLPYKLYYDLPEIPLS
                     LDIPLTFEDQKTPVKPDLRKIGHFLWDVYGITQFSQTVTPINSTERKLDAMHSYRRFAP
                     SGGALYPNELYVYLKIDDIPDGVYHFDVSHHRLVLLREGNFDDYLTRSLGYRCDLTTCL
                     GTVFISTMFWKNFFKYNNFSYRLQGLDAGVLMGQLLEVAKRFGFASGVYYQYLDRAINH
                     LLGLKEDEESVYSAIPLTIEPTMWSGNRYGENVSASDLLREIPPIQVKHYVRTKKMKEF
                     PMLIKMNEAAMIHSTPFHWVKKKERRKDDGKEIPLPLVNRLSYDFLSTCRKRFSPDMDF
                     NLTKISAKQLAYLLYEATESFSYRNDLEAEHLEKLRVSIFVCLYNIEGIPNGAYYYNNT
                     NHSLYLVNAGDHRLQLQSGLSLDNVNLLQVPITLHIVGDKDYNKNSLGYRGYRIQQMEA
                     GILVQKLLLAASLLDMGGHPLLGFDVNLTDHIYNLSPSGKTSLIQIPIGPYRQRPWLMG
                     RLCT"
     gene            complement(2884..4831)
                     /locus_tag="MVE64_RS01575"
                     /old_locus_tag="MVE64_01575"
                     /pseudo=""
     CDS             complement(2884..4831)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013877478.1"
                     /locus_tag="MVE64_RS01575"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="MVE64_01575"
                     /product="TOMM precursor leader peptide-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MGADLLVVGEGVLADLFCTELSSDFLIVRKTHINSSISETIDLAL
                     VLQDHWTPALHQNAENVFRESQIPWLRGFISFGEGIIGPLVQPGIPGCSQCADLRRVLT
                     GTHRQEMWSMQKELESSGKTQQGAWASRTGLLQMVQLLKVEVERWIQGGKTFSEKRLGF
                     INLKTLRSSWHSFLPVPQCTVCCAIPDDSPELNFALLFRKVRKLARKVTELVH"
     gene            complement(5001..6731)
                     /locus_tag="MVE64_RS01580"
                     /old_locus_tag="MVE64_01580"
     CDS             complement(5001..6731)
                     /GO_component="GO:0005575 - cellular_component [Evidence
                     IEA]"
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /GO_process="GO:0030152 - bacteriocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03693"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Heterocycloanthracin_C_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thiopeptide_F_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03693.1"
                     /locus_tag="MVE64_RS01580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MVE64_01580"
                     /product="putative thiazole-containing bacteriocin
                     maturation protein"
                     /protein_id="WP_247343113.1"
                     /sec_met_domain="TIGR03693 (E-value: 1.1e-224, bitscore:
                     745.5, seeds: 2, tool: rule-based-clusters)"
                     /sec_met_domain="Heterocycloanthracin_C_RRE (E-value:
                     9.5e-50, bitscore: 164.8, seeds: 5, tool:
                     rule-based-clusters)"
                     /sec_met_domain="Thiopeptide_F_RRE (E-value: 5.7e-13,
                     bitscore: 46.9, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNLTPSMRLKVKRDTFFLPEANSGVYFRNNVSSFRMEGSMIDKW
                     VEKLIPMFNGEHTLESLTNGLTGPYRDRVFAIAETLYQNGFVKDVSRDQPYSLSEQVVK
                     MYASQIEFIDSFVESAAFRFQVYREAKVLAVGSGDMFVSLVSSLLDSGLPKFQTFITDR
                     SKTNIQRLQELVDHARALDSEVAVEEILQGSWKKIVEPFDYILYVSQEGNIEELNLLHT
                     ICREENKIFIPALCLQQVGLAGPFVFPNSEYCWKSAWSRLHKSELYKEIHISNCSTTAR
                     AMLANLIVFELFKNMTGVKEREQNNQIFLLDLETMEGNWHTFFPHPVMTGNTIARLIDN
                     EDARIQQDLNTQEAETLILFFNKLTSKSTGIFHTWDEGDLLQLPLAQCRVQPIDPLSDG
                     PAELLQSTICSGLTHEEARREAGLAGIEAYVNQLSTVFDLPTQVYIGAGETLTESVCRG
                     LQRYLVQEMKRNQANATNTFSRVKLNSVEDERCRYYLQSLSTIQGEPNIGVGEEVSGFP
                     VVWIGTKNGWYGSVDLNITLALRKALQQALFNQQNQEDPLTPQAYSYHQQYLTKNHLNN
                     "
     aSDomain        complement(6435..6731)
                     /aSDomain="Heterocycloanthracin_C_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing C protein in a
                     heterocycloanthracin LAP cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_MVE64_RS01580_Heterocycloanthracin_C_
                     RRE.1"
                     /evalue="5.30E-52"
                     /identifier="RREFam011.1"
                     /label="MVE64_RS01580"
                     /locus_tag="MVE64_RS01580"
                     /protein_end="99"
                     /protein_start="0"
                     /score="164.8"
                     /tool="antismash"
                     /translation="MTNLTPSMRLKVKRDTFFLPEANSGVYFRNNVSSFRMEGSMIDKW
                     VEKLIPMFNGEHTLESLTNGLTGPYRDRVFAIAETLYQNGFVKDVSRDQPYSLS"
     aSDomain        complement(6465..6713)
                     /aSDomain="Thiopeptide_F_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing F protein in a thiopeptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_MVE64_RS01580_Thiopeptide_F_RRE.1"
                     /evalue="3.20E-15"
                     /identifier="RREFam004.1"
                     /label="MVE64_RS01580"
                     /locus_tag="MVE64_RS01580"
                     /protein_end="89"
                     /protein_start="6"
                     /score="46.9"
                     /tool="antismash"
                     /translation="SMRLKVKRDTFFLPEANSGVYFRNNVSSFRMEGSMIDKWVEKLIP
                     MFNGEHTLESLTNGLTGPYRDRVFAIAETLYQNGFVKD"
     misc_feature    6861..6874
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.75"
                     /tool="antismash"
     gene            complement(6881..7207)
                     /locus_tag="MVE64_RS01585"
                     /old_locus_tag="MVE64_01585"
     CDS             complement(6881..7207)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071368.1"
                     /locus_tag="MVE64_RS01585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MVE64_01585"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_247343115.1"
                     /transl_table=11
                     /translation="MNNFQQDLQMLNLGDYQATQAVPYDQNQYYHDDERFFFGLGNIFR
                     CFSCFSCYGCSSCFSCYNCGGGYCGGGGRCGGGGYCGGGGRCGGGGYCGGGGRCGGGGR
                     CGGR"
     gene            7475..7675
                     /locus_tag="MVE64_RS01590"
                     /old_locus_tag="MVE64_01590"
     CDS             7475..7675
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MVE64_RS01590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MVE64_01590"
                     /product="hypothetical protein"
                     /protein_id="WP_247343117.1"
                     /transl_table=11
                     /translation="MSSFEKSIQERLKIPKQTINGKEVILKEGDLCLLDTNVIHSIDQA
                     GENDIVINIMVRTSYFDSNFT"
     gene            7787..8173
                     /locus_tag="MVE64_RS01595"
                     /old_locus_tag="MVE64_01595"
     CDS             7787..8173
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /locus_tag="MVE64_RS01595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MVE64_01595"
                     /product="AraC family transcriptional regulator"
                     /protein_id="WP_247343120.1"
                     /transl_table=11
                     /translation="MLLLFTDLLRIYHDSPSKIDQPTFTKGVISEILDYMEKNYETIIL
                     EKTANYFHFHPNHLTRLLKQNLNKTFIELSHQLKNACTMLENTDFTVDQIAHKTGYAKV
                     TFFYKTFKKFHGVTPAKYQKKTND"
     gene            complement(8248..9945)
                     /locus_tag="MVE64_RS01600"
                     /old_locus_tag="MVE64_01600"
     CDS             complement(8248..9945)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1061: methyl-accepting
                     chemotaxis protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /locus_tag="MVE64_RS01600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MVE64_01600"
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="WP_247343123.1"
                     /transl_table=11
                     /translation="MKDLFRFKSIKTKLLISFLFITLLVLGFGAYLISSMKQMENHTRE
                     ISSQDIPLMASEFSLLGILTQQQSELRGYLLTGDEKYKQIYFALKDPSLAIQKDLLNRT
                     NETAIRDVSTQTEDIYKIIEEKYFSAIESGDIDKAESILQNQIEPVLTKSVEQMTALAL
                     AQGDQSKENGIQALKQAETSTYIAIISGVILLAFIISFSIIMPKQLVKTINQLKTRMDL
                     IANGDLSLEPLATKSRDEIGGLITASNLVNENLKNMLRKISDVSDTLSVQSGSLTNSSN
                     EVKETSNQVSLTMQELATGSETQAGISTDLSSSMVTFLNEIKEANSSGESVYATSQNVI
                     TQTIEGSELMNASISQMGKIDTIVKEAVQKVTGLDKQSQEISKLVGVIKEIADQTNLLA
                     LNAAIEAARAGEQGKGFAVVADEVRKLAEQVSISVTDITEIVGSIQNETQSVTKSLQGG
                     YQEVELGKNQIISTGDTFKQIDHSLAQVVSGIKEISQSLNQLTDNSDMINKSIEEIAAV
                     SEESAAGIEETAASVEQTSHSMEQVATSAGNLNLLAKDLKDLISQFKL"
     misc_feature    complement(10143..10157)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 23.18"
                     /tool="antismash"
     gene            10463..11395
                     /locus_tag="MVE64_RS01605"
                     /old_locus_tag="MVE64_01605"
     CDS             10463..11395
                     /NRPS_PKS="Domain: Abhydrolase_1 (33-180). E-value:
                     4.5e-13. Score: 41.3. Matches aSDomain:
                     nrpspksdomains_MVE64_RS01605_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017552067.1"
                     /locus_tag="MVE64_RS01605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MVE64_01605"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_247343126.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 3.3e-07, bitscore:
                     29.4, seeds: 455, tool: rule-based-clusters)"
                     /sec_met_domain="PF00561 (E-value: 8.1e-08, bitscore: 30.4,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAFEELSFQSFNQKDHIKAWIYTPIRKPRGIVQLVHGFGEHSRRY
                     LHMILKLNEAGFVVAADDHVAHGKTALDSGNWSDWGDKGYMTMAEDEHILRTIVQEKYP
                     DLPFFMFGHSMGSMIARGYAATYGEGMAGLLLCGTSGVFPKAAEMVPLLKKLIDEGKGE
                     EINPDLLGEFLGWMTERIENPSTPNDWIAGDPDVVADHANDPFNNFISPPNIRSLYYFG
                     MMMETIVGTQWAEKVPVSIPVYNIAGDLDPVGLYGEGVYAVSNWLGETGHRVKTKIYSG
                     YRHEIHNYRDIRDEVEDGIIDFMNTIIGE"
     aSDomain        10562..11002
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MVE64_RS01605_Abhydrolase_1.1"
                     /evalue="4.50E-13"
                     /label="MVE64_RS01605_Abhydrolase_1.1"
                     /locus_tag="MVE64_RS01605"
                     /protein_end="180"
                     /protein_start="33"
                     /score="41.3"
                     /tool="antismash"
                     /translation="LVHGFGEHSRRYLHMILKLNEAGFVVAADDHVAHGKTALDSGNWS
                     DWGDKGYMTMAEDEHILRTIVQEKYPDLPFFMFGHSMGSMIARGYAATYGEGMAGLLLC
                     GTSGVFPKAAEMVPLLKKLIDEGKGEEINPDLLGEFLGWMTER"
     gene            complement(<11474..11582)
                     /locus_tag="MVE64_RS01610"
                     /old_locus_tag="MVE64_01610"
                     /pseudo=""
     CDS             complement(<11474..11582)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002111302.1"
                     /locus_tag="MVE64_RS01610"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="MVE64_01610"
                     /product="VOC family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MVQLRIARQQINSRKLLPFMKKV"
ORIGIN
        1 gacgaaatga gatgtgtttt gcttcatgag ctggttcatt gtaaacgtaa agatctactc
       61 attaattatt tcctgggtct gtcgagaatc gtttattggt ttaatcctct cgtttggaat
      121 tttttacata acatgaaaac agagatggag attacttgcg attatgctgt tttgaaaaag
      181 ctggatgagg aatctcaact taaatacgga gaggttattt taaaatttgc ttcacaagca
      241 aaacaatcat tgccgttgat tgctgcttca gaaattagta gctcgtataa acaagtaaaa
      301 cagcgtattg tgacaattgt aaatttcaaa gtagaatcaa aacaacagaa aataaaaggt
      361 tcgctaattt ttattgctgt tcttgctatc attttaagca gtattccttc aatatctgta
      421 ttagccttta acaaagatca acattctttt tcagaacaaa atgtggtgta caaagattat
      481 cagactcttt ttcatgatct tacaggaagt gctgtagtat atgacaccaa aatggatcag
      541 tacatgattt ataatgaaga tgaaagtaca acaagatatg ctcccaattc tacttataaa
      601 atattcagtg cattgtttgc attagaaaac gagattatta aaaaagaaca ttcgaaaatc
      661 gcctgggatg gaactctaca caaatatgat aactggaatc aagaccaaga tttgtttaac
      721 gcaatgaaaa attcgacaac ttggtatttt caacatcttg atcagcagct tggtaaaaag
      781 aaattgcaaa cttactacga acaaattggc tatggaagca aagatatatc aaatgatata
      841 gctaattatt ggatggatgg tcacttaaaa atctcccctg tagagcagat tgatatgtta
      901 aaaagatttt ataaaaatga actctctttt gatcaatcga atatacaaac tgttaaagac
      961 tcgttacttt tagaagattt taatggtctt cgtttatctg gtaaaacggg aaccggagat
     1021 gtaaacggag aaagtgttga tggctggttt ataggatatg tagagacagc ggataataca
     1081 tttttctttg ccgttcatat aaaaggagaa aaaaatgcgg gaggaagcga tgcagtgaag
     1141 atcgcccttt ccattttgga gaaggaagga atttatagat ctattctctt ggaatcttca
     1201 taacttttct ataaaaaatg ctcccccttc aggttagcaa taataaaaac gttccttacc
     1261 cttttgtaag gaacgttact tcaatctgat ctaaattctt ttacgtacaa agtcttccca
     1321 tcagccaagg tcgttgtcga taaggaccga ttggaatttg aatcaagctt gtttttccag
     1381 atggactcaa gttgtaaatg tgatctgtta gattcacatc gaaaccaaga agcggatgtc
     1441 ctcccatatc tagaagtgaa gctgctaata acaatttttg gactagtatc cccgcttcca
     1501 tttgttgaat tcgatatcct ctgtaaccaa gtgaattttt attataatcc ttatcaccaa
     1561 caatatgtaa ggtgatagga acctgtagta aattaacatt atctaaagac aaccctgatt
     1621 gcagctgtag ccgatgatct ccagcattca ccaagtataa tgaatgatta gtgttgttat
     1681 agtaataagc tccattaggg ataccttcaa tattatacaa acaaacaaaa atagagacac
     1741 gtaatttttc tagatgttct gcctctaaat cgtttcgata agagaaagat tctgttgctt
     1801 catatagaag gtaagcaagt tgttttgcac ttatttttgt caagttaaaa tccatgtcag
     1861 gtgaaaaacg ctttcggcag gtagacaaga agtcatacga aagacgatta acgaggggca
     1921 aaggtatctc cttaccatca tcttttcttc tttccttttt cttgacccag tgaaaaggtg
     1981 tagaatggat catagcagct tcattcattt ttattaacat gggaaattct ttcattttct
     2041 ttgtacgaac gtaatgttta acctgaattg gtggtatttc tcgtaacaag tccgaagctg
     2101 atacattttc tccataacgg tttccagacc acatcgttgg ttctattgtt agtgggatgg
     2161 ctgaatatac gctttcttca tcttccttaa gaccaagtag atgattaatg gcacgatcaa
     2221 gatattgata gtacacacct gatgcaaatc caaaacgttt tgctacttct aataactgcc
     2281 ccattagcac acctgcatct aacccttgta gccggtaaga aaaattattg tacttgaaaa
     2341 agtttttcca aaacatcgtt gagataaaaa cagttcctaa gcaagtagta agatcacatc
     2401 ggtatccaag tgaccgggta agataatcat caaaatttcc ttcacgcagt aaaactaaac
     2461 ggtgatgtga gacgtcaaaa tggtaaacac catccggtat atcgtcaatt tttaaatata
     2521 cgtataattc gtttggatat aacgctccac ctgaaggagc aaacctccta taggaatgca
     2581 tcgcatccag ctttctctcg gtagaattta tcggagtaac agtttgacta aattgagtaa
     2641 ttccataaac atcccaaaga aagtgaccga ttttccgcag atctggtttt acaggggttt
     2701 tctgatcttc gaaagttagc ggtatatcca atgaaagtgg aatttcgggt aagtcatagt
     2761 aaagcttgta agggagtgga ccatcctccc aatccacttc ccagtttgga atgcttgctt
     2821 tatcaatgtc aaaatgtaag ttatgcagaa actcatcaag aatcaccctt ccagcctccc
     2881 ttcttatgga aacgggtgtg gaagtggatt aagcttttca cgtgtcagag gttctgttgt
     2941 ataccccagt tccataggaa cacgtaatat cctctctaac cctgatacac gctttagatg
     3001 gtgtccaaat gtcattggaa ccatcccagg aattaatact ttaacacaat gtaaaccatt
     3061 tcgttttact tcgggtgttg tttgatcaat aacaataaca tccaggttta cgttctttaa
     3121 tgcttgaaga atatctcgaa gatcatcggt aaggtcagcg tgacttgact tccacttgaa
     3181 ttcttcgtta aacgttttac ttttttgttt attatgaagt aaaaaatgta gtcgctcttc
     3241 agcttgtggt aagccataca acatgccatg gtcatccatt tgttgaacga gggaagaatc
     3301 gtgtaacata cgcacataat tctctttatt ttcctcaaat ttctcatcaa gattcagcat
     3361 cataccagcc agctcatgaa cagcggtttt cacagcacgc accggatcca aatggcccct
     3421 gctgcacaga ttagatttaa ccctttttgc tttctgtttt ttgcaagtgc aataatgctt
     3481 gggattccgt gttccattgt tgagttatat atatgaaggt catatcctcc gaccgctctc
     3541 attctgtcaa tcattaagac tagttcttca tcttcagaag agtacggatc aagacgaggt
     3601 aagggtaatt gtgcatacca ggtcataaga aacgaatcac gctcgaccac ttccataatg
     3661 ccgtaaaaaa tagcttcttc taaactacct cccaatgcac atccgttgga agtttcataa
     3721 acaaatccct gtgatccgca tcccaaacta taataagcaa gtaactcagg cacgagaatc
     3781 ggtcgttcct gtaacaacga atagccccat acccatttca ttggacggtc agggtcaaat
     3841 ttttcaaagg gaaagcctga gccttgatac tgatcttctt catgtacacc aactaatttc
     3901 gggtttaagg cttgattttt caaattttca tagttatcaa atactactgt ccgtttccca
     3961 cggggctcaa gcccacagta tcgttccaat ccttctaaaa tcgcagttaa ttcacttgca
     4021 gcatacgaat ttgtacgacc tgccgttccc tcatccccct caaacaaagg caaattcaca
     4081 cttacatctg caaaaggagg tacaagatta tacattttat tattgagcaa acctgttcga
     4141 gcatcaagat agtccttgtc taacacgctt tttagatcat ctaatgaacg agttcggtaa
     4201 ctttccttgc taactttcgg acttttctga agagcaatgc gaaatttaac tcaggagaat
     4261 cgtcaggtat ggcacagcat actgtacatt gaggaacagg caagaaggag tgccatgaac
     4321 ttctcaatgt ttttaggttg ataaaaccaa gtcgcttctc tgaaaaggtt tttccccctt
     4381 gtatccacct ctcgacctct actttaagga gctgaaccat ttgtaacaac cctgtccgtg
     4441 aagcccacgc tccctgttgt gtttttccag aactctctaa ttctttttgc atgctccaca
     4501 tctcttgacg gtgagttcct gttaagactc gtcgtagatc agcacattgc gaacaacctg
     4561 gaattcccgg ctgtaccaat gggcctataa tgccttctcc aaatgatata aagcctcgaa
     4621 gccacgggat ctggctttct cgaaacacgt tttctgcgtt ttgatgaaga gcaggggtcc
     4681 aatgatcttg caggaccaaa gccaaatcaa tggtttcaga aatgcttgag ttaatatgtg
     4741 ttttacgaac aattagaaaa tctgaggaaa gctccgtaca aaaaagatct gctaacacgc
     4801 cctcaccaac aaccaacaaa tctgcaccca tcttcattcc tcctctcgaa ccacaattcc
     4861 tacgatccct gctaatcctt ctttaaagac tggctctgct tcaatttcat aaattttaaa
     4921 ttgttttttg ttccgactca atacttcctt agcagaatgc aatagaacag aatgattttg
     4981 attatgacca tcaggaatat ttagttgttg aggtgatttt tcgtcaagta ctgctgatga
     5041 taagaataag cctgtggtgt taaaggatct tcttggtttt gttggttaaa aagggcctgt
     5101 tgcaatgctt ttcttaatgc cagggttata ttgagatcta cacttccata ccatccattt
     5161 ttcgtaccaa tccatacgac aggaaatcca gatacttcct cacctactcc gatattgggt
     5221 tcaccctgaa tggttgataa agattgtaaa taatacctac accgctcatc ttcaacacta
     5281 ttcaacttta ctctagaaaa agtatttgta gcgtttgctt gatttctttt catctcctga
     5341 actaaataac gttgtaatcc tcgacaaaca ctttctgtca atgtttctcc tgctccaata
     5401 tatacttgtg ttggcagatc gaacacggtc gataattgat taacatatgc ttcaattcca
     5461 gcaagacctg cttcccttct agcctcctca tgtgttagtc ctgagcaaat cgtactttgc
     5521 aataactcag caggtccatc agacaatgga tcgattggtt gaactcggca ctgagctagc
     5581 ggaagctgaa gcaaatcacc ttcatcccaa gtatgaaaaa tccctgttga tttcgaggtt
     5641 agtttattga aaaaaagaat taatgtctct gcttcttgag tgttcaagtc ttgttgaatt
     5701 cgtgcatcct cattatctat aagtctagct attgtattac cagtcataac aggatgagga
     5761 aaaaatgtat gccagtttcc ttccatcgtt tctaaatcta gtaaaaagat ttggttattc
     5821 tgttctcgtt cctttactcc ggtcatgttt ttaaacaatt caaagacaat cagattagct
     5881 agcatcgctc tagctgtagt ggaacagttt gaaatgtgta tttctttgta gagttccgat
     5941 ttatgaagcc tgctccaagc ggatttccaa caatactctg aatttggaaa aacgaaaggc
     6001 cctgccaaac caacctgttg taaacataaa gccggaatga atatcttgtt ttcctctctg
     6061 caaatggtgt gaagaagatt tagctcctca atattcccct cttgtgacac gtacaagata
     6121 taatcaaatg gctccacaat tttcttccaa gaaccttgca agatctcctc tactgccacc
     6181 tccgaatcta gtgctcgggc atggtcaacg agctcctgca gccgttgaat attcgtttta
     6241 ctcctgtcag taatgaatgt ttgaaactta ggtaacccgg aatctagtaa tgatgaaacc
     6301 aatgaaacaa acatatctcc tgagccaact gccaacactt ttgcctcccg atatacttga
     6361 aatcggaacg cagcagattc tacaaaacta tcgataaact caatttgtga tgcatacatt
     6421 ttcacaacct gttctgacaa tgaatacggc tggtccctgc ttacatcttt tacaaatcca
     6481 ttttgataca atgtttccgc gattgcgaac actcgatctc gatacggccc tgttaacccg
     6541 tttgttaaac tctctaatgt atgttcacca ttgaacatcg gtattagttt ttcaacccat
     6601 ttatcgatca tacttccttc catacgaaac gaacttacat tattccgaaa atatactcca
     6661 ctattagctt cagggagaaa aaacgtgtcc cttttcacct tcagacgcat agaaggggtc
     6721 aaattggtca taacgatcct ccttagccgt agctaccaat atctttccat ttcattacta
     6781 acatcttatg tactgtaaat tgtcccttat gttatttttc aaagagaaag cctctacatc
     6841 aatatgtaaa ggcctgtcgt attttttaaa agaatacctt ttatctgcct ccgcagcgac
     6901 ctccgccacc acaacggcct ccaccaccgc aataacctcc gccgccacaa cggcctccac
     6961 caccgcaata gcctccgccg ccacaacggc ctccaccacc acaatagcct ccgccacaat
     7021 tatagcagct gaaacagcta gaacagccat agcaactaaa acagctaaaa cagcgaaaaa
     7081 tatttcctag tccaaagaaa aatctttcat catcatgata atattggttc tggtcataag
     7141 gaacggcctg agttgcttgg taatctccaa gatttaacat ctgaagatcc tgttgaaaat
     7201 tattcattca gtaaaacctc ctcaaaatag ttgcctaaaa aaagtgacaa gaaacaaaca
     7261 cacaaagata agctaaactt ctgggaaaaa catacttctt caacaaatta tgtaaattgg
     7321 gtggctgttg ctactggtga aaaagcctaa attgagtttt tatgaaagct tggggaatgc
     7381 ataaaatttc cttataatat agagtaaata gaaaacgatg aaaggggaaa ttctaattga
     7441 atacctcctc acttcttgaa aaacaattaa ggatatgagc agctttgaaa aaagtattca
     7501 ggaacgccta aaaatcccaa aacaaacgat taatggaaaa gaagtgattt taaaagaagg
     7561 agatttgtgt ttacttgata cgaacgtgat tcattctatt gatcaagctg gagagaatga
     7621 tatcgtgatt aatattatgg ttaggacaag ttattttgat agcaatttta cttagaagat
     7681 tgtcaggaaa tgatttactc acggactttt ttgttcatac aatttacaag cattattaga
     7741 ttacacggat ccgcaattat gttcaaaaga agtaattaat tcctatatgc ttctactttt
     7801 tacggatttg ctaagaattt atcatgattc tcctagtaaa attgatcaac caacgtttac
     7861 aaaaggtgtt atatcggaaa tcttggatta tatggaaaaa aactatgaaa ctatcatttt
     7921 agagaaaaca gccaattact ttcattttca tccaaaccat ttaacaaggc tacttaaaca
     7981 gaatcttaat aaaacgttta ttgaactatc acatcaatta aaaaatgcgt gcaccatgct
     8041 tgaaaacaca gattttactg tagatcagat tgctcataaa acgggttatg caaaagttac
     8101 ctttttttat aaaaccttca aaaaatttca tggtgttaca cctgctaaat atcaaaagaa
     8161 aaccaacgac tgaattattg ataagtcgtt ggttttcttc ttcaaaaata tgtttatttt
     8221 tttcttaatc ctcactaata tctatcatta aagtttaaat tgtgaaatta aatctttaag
     8281 gtcttttgca agaaggttta agttacctgc actagtagct acctgttcca ttgaatgact
     8341 tgtttgctcg acagaagccg ctgtttcttc gattcctgca gcagattcct ctgaaacggc
     8401 tgctatctct tcaattgatt tatttatcat gtcactgtta tctgttaatt gatttaggct
     8461 ttgtgatatt tcctttatac ctgaaacaac ttgggccaaa gaatgatcaa tttgcttaaa
     8521 tgtgtcacct gtactaataa tttgattttt cccaagctct acttcttgat atcctccttg
     8581 taaagacttt gtgacgcttt gcgtttcatt ttgaatacta ccaacaatct cggtaatatc
     8641 tgttaccgaa atcgatactt gctcagctaa cttccgaacc tcatcagcaa caacagcaaa
     8701 tccttttcct tgttcacctg cacgcgctgc ttcaattgcc gcatttaatg ccaataaatt
     8761 tgtttgatca gctatttctt taataactcc tacaagctta gaaatttcct gtgattgttt
     8821 gtctaatcct gtgacttttt gtacggcttc tttcacgatt gtatcaattt tgcccatctg
     8881 tgaaatcgaa gcattcatca gttcactgcc ctctattgtt tgcgtgatga cattctgaga
     8941 agtagcataa acagactccc cgctactatt tgcttcctta atttcattta aaaatgtcac
     9001 catagaagat gaaagatccg tgctgattcc agcttgtgtt tctgaacctg ttgcaagttc
     9061 ctgcattgtt aatgaaactt gattactcgt ttcctttact tcattagagc tgtttgttaa
     9121 agaaccactc tgaacagaga gagtatcgga cacatcactt atttttctta acatattttt
     9181 caaattttca tttacaagat tagatgctgt aattaatcct ccgatttcat cccttgattt
     9241 tgttgcaagt ggttctaggc ttaaatctcc attagcgatt aagtccatac gtgtttttaa
     9301 ttgattgatt gtttttacaa gctgtttagg cataatgata gaaaaggaaa taatgaaggc
     9361 taagagaata acacctgaaa taattgctat atatgttgag gtttcagctt gcttcaaagc
     9421 ctgaattcca ttttctttac tctggtctcc ttgagctaaa gctaacgcag tcatttgttc
     9481 aacactcttt gttaaaacag gctctatctg attttgaaga atagattctg ccttatcaat
     9541 gtctccgctt tcaatagcag agaagtattt ttcttcgatt attttataaa tatcctccgt
     9601 ttgcgtggaa acatccctaa tagctgtttc attcgttcga tttaataaat ccttttgaat
     9661 cgctagactt gggtccttta aagcaaaata tatttgttta tatttttcgt caccagtaag
     9721 taaataacct cgtagttcac tttgttgttg agtaagtatt cctaacaagc taaactctga
     9781 cgccatcaaa ggtatatctt gtgaagaaat ttctctcgta tgattttcca tttgtttcat
     9841 tgaactaatt aagtaagcac caaatcccaa aactagtaat gtaataaaaa gaaaagaaat
     9901 taataatttg gtttttatgc ttttaaaccg aaataagtct ttcatcacca aaccccattc
     9961 caaatatatt tgtctattct taacatcggt ttctctatgt ctttttccat aggaatgtat
    10021 tcctatattg agaacctgtt tggctagttc attttcggtt aaaatcaaat aggaaccaca
    10081 aacgatttac tcataagaaa tgtcgtggtt tattttagtt ttatatatct agactttagg
    10141 aatgaacatg atttacaccc aaggtaccgc cattcaaact ctccgtatgg tacggtaaac
    10201 tgcaaattac tggaatcttc atcatagatc agctatgtat atcccttaaa ttatgtgact
    10261 ttagaaaata gtaaaacatg gaaattacta ccttcctttt aattgacagc gttatcattc
    10321 cacaattata cttatatata agttaataac tgtgaagaga acacggagac acactagata
    10381 ccgaactaag caattattta gtgtgtcttc tttttgtttt cgtagaaaaa tctactatta
    10441 taccaaaact aggaggttca ttatggcatt tgaagaacta agtttccaat cgtttaatca
    10501 aaaagatcat attaaggcat ggatttacac ccctattcgc aaaccaagag gaattgttca
    10561 gcttgtccat ggttttggag agcattcgcg tcgatacttg cacatgatct tgaaattaaa
    10621 tgaagctgga ttcgtcgtag ccgcagatga tcatgttgct catggaaaaa cagcccttga
    10681 ttcaggtaac tggagtgatt ggggcgataa agggtatatg acaatggcag aagacgaaca
    10741 tatacttcga accattgtcc aggagaagta tcctgattta ccttttttca tgtttggaca
    10801 tagtatgggt tctatgattg caaggggtta tgccgctact tatggagaag gaatggcggg
    10861 actcctttta tgcggaactt ctggtgtttt tccaaaagca gctgaaatgg tacctctttt
    10921 gaagaagctt atagatgaag gaaaaggtga agaaattaat cctgatttgc ttggagaatt
    10981 tttaggttgg atgactgagc gaattgaaaa tccttctaca ccaaatgact ggattgccgg
    11041 tgaccctgac gtggttgctg accacgcgaa tgatccattc aataacttta tatcaccacc
    11101 aaatatccgt tcattgtatt attttggtat gatgatggaa actattgttg ggacacagtg
    11161 ggcagaaaaa gttcctgtct ctattccagt ttacaatatt gccggagatc tagatccggt
    11221 tggcctctat ggtgagggtg tttatgctgt atctaattgg ttaggagaaa ctggacatag
    11281 ggtcaaaact aagatttatt ctggctatag acatgaaatc cataattaca gggatattag
    11341 ggatgaagtt gaggatggaa taatagattt tatgaatact attataggag aataatcttt
    11401 tgttattgat atattttagg atgtgaggac agagcgatgt cttcatgaaa cgggacagtg
    11461 tacctgtccc tttatcatga ttttcaaatg aagccccact ctatttaaac ctaaaccttc
    11521 ttcataaaag gtaatagctt cctcgaattt atctgttgtc ttgcaattcg aagttggacg
    11581 attcttttac ttcctcacac attaagaaaa aagatgacca attgtttgtg gttcatgttc
    11641 atttcttctg ctccactcta gaagaatata acgagaaaaa accagtgttg tatggcttat
    11701 catgaagtta taagatcttc cttgaaattc t
//
