LOCUS       NZ_CP095376            10393 bp    DNA     circular CON 06-NOV-2024
DEFINITION  Amycolatopsis sp. FBCC-B4732 chromosome, complete genome.
ACCESSION   NZ_CP095376
VERSION     NZ_CP095376.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis sp. FBCC-B4732
  ORGANISM  Amycolatopsis sp. FBCC-B4732
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 10190959)
  AUTHORS   Choi,A.
  TITLE     Complete Genome Sequence of Amycolatopsis pretoriensis strain
            FBCC-B4732
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10190959)
  AUTHORS   Choi,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2022) Bioresources Collection & Research Team,
            Nakdonggang National Institute of Biological Resources, Donam 2-gil,
            Sangju-si, Gyeongsangbuk-do 37242, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 75.1x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023008405.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 10:39:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,364
            CDSs (total)                      :: 9,298
            Genes (coding)                    :: 9,177
            CDSs (with protein)               :: 9,177
            Genes (RNA)                       :: 66
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 50 of 121
            Pseudo Genes (incomplete)         :: 81 of 121
            Pseudo Genes (internal stop)      :: 14 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:14:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8979348
            Orig. end                         :: 8989741
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095376.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from NNIBR.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10393
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="37"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10393
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10393
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5393](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5393
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(1709..1724)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.29"
                     /tool="antismash"
     misc_feature    1892..1907
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.87"
                     /tool="antismash"
     gene            3209..3718
                     /gene="ectA"
                     /locus_tag="MUY14_RS40720"
                     /old_locus_tag="MUY14_40720"
     CDS             3209..3718
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020641771.1"
                     /locus_tag="MUY14_RS40720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_40720"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_247017731.1"
                     /transl_table=11
                     /translation="MSVTHLIESPTKADGAKLWRIARDSAKLDLNSPYAYMLWCRDFAE
                     SSVVAREDGEAVGFVIAYRRPDEPEAALVWQVAVDASQRGKGLAGALLDALYTSLVADG
                     VRYLETTITPDNEASIRLFASFAKRWNAAVETSVLFAGEDFPEAGHLAEELYRIGPLTP
                     RKDPGE"
     gene            3734..4987
                     /gene="ectB"
                     /locus_tag="MUY14_RS40725"
                     /old_locus_tag="MUY14_40725"
     CDS             3734..4987
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-354). E-value: 2.3e-71.
                     Score: 232.5. Matches aSDomain:
                     nrpspksdomains_MUY14_RS40725_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230319.1"
                     /locus_tag="MUY14_RS40725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_40725"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_247017733.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.2e-71, bitscore:
                     240.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIFEELESEVRSYSRGWPVVFDRAQGSHLYDENGKAYLDFFAGA
                     GALNYGHNNPRLKQALIDYIQRDGVTHALDMFTVAKRDFLQTFRDKILDPRNMNYKVVF
                     PGPGGANAVEAALKLARKVTGKESVINFTNAFHGMTLGALSVTGNSMKRGAAGIPLVHA
                     TPMPYDAYFDGAMPDFLYFEKLLEDSGSGLNEPAAVIVEGVQGEGGINAARLEWLKGLD
                     DLCKRHNILLILDDVQMGCGRTGPFFSFEDAGISPDIVCLSKSIGGYGIPMALTLIKPE
                     LDVWEPGEHNGTFRGISPAFVTATEAINAYWSDDELEKSTKAKGERIAAAFSGIVEAYP
                     DANLVAKGRGLARGLGFENGDLAGRVCAEAFARGLLMETSGPDGEVMKLLPPLTLTDDE
                     LTQGLSIIDESIKAVLKK"
     aSDomain        3797..4795
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MUY14_RS40725_Aminotran_3.1"
                     /evalue="2.30E-71"
                     /label="MUY14_RS40725_Aminotran_3.1"
                     /locus_tag="MUY14_RS40725"
                     /protein_end="354"
                     /protein_start="21"
                     /score="232.5"
                     /tool="antismash"
                     /translation="FDRAQGSHLYDENGKAYLDFFAGAGALNYGHNNPRLKQALIDYIQ
                     RDGVTHALDMFTVAKRDFLQTFRDKILDPRNMNYKVVFPGPGGANAVEAALKLARKVTG
                     KESVINFTNAFHGMTLGALSVTGNSMKRGAAGIPLVHATPMPYDAYFDGAMPDFLYFEK
                     LLEDSGSGLNEPAAVIVEGVQGEGGINAARLEWLKGLDDLCKRHNILLILDDVQMGCGR
                     TGPFFSFEDAGISPDIVCLSKSIGGYGIPMALTLIKPELDVWEPGEHNGTFRGISPAFV
                     TATEAINAYWSDDELEKSTKAKGERIAAAFSGIVEAYPDANLVAKGRGLARG"
     gene            5001..5393
                     /locus_tag="MUY14_RS40730"
                     /old_locus_tag="MUY14_40730"
     CDS             5001..5393
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071625.1"
                     /locus_tag="MUY14_RS40730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_40730"
                     /product="ectoine synthase"
                     /protein_id="WP_003071625.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.3e-54, bitscore:
                     181.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLVRTLDEITDTDADIKTENWRSKRIILAKEGVGFSVHETTLYAG
                     TVNDFWYANHIEAVFITSGEGEIEDLATGKVYELKPGTLYLLNDHDKHQVRPKTEIKCV
                     CVFNPPVTGREVHDENGVYPLITEDA"
     gene            5425..6327
                     /gene="thpD"
                     /locus_tag="MUY14_RS40735"
                     /old_locus_tag="MUY14_40735"
     CDS             5425..6327
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631927.1"
                     /locus_tag="MUY14_RS40735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_40735"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_247017735.1"
                     /sec_met_domain="PhyH (E-value: 2.5e-34, bitscore: 118.5,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLMDTRVDDTYPTRITGTPAHLPRAHPTVWGTEADGPIDAATLA
                     NHETNGYTVVEDMLSVGEVQAYWQELVRLSSDAELARDERVITEAKTGEVRSIFDVHEI
                     SALIAELVRDPRVLDRARQLLGSEVYIHQSRVNYMPGFKGTGFYWHSDFETWHAEDGMP
                     SPRAVSCSIALTDNYPFNGGLMIMPGSHRTFVQCAGETPEANYKASLKDQRVGVPNEDD
                     ITRMAAEHGIDQFTGQAGSALWFDSNIMHGSGNNITPYPRSNIFLVFNSVENALQEPFA
                     ASAPRPAFIAGRDSTPISR"
     gene            6759..9536
                     /locus_tag="MUY14_RS40740"
                     /old_locus_tag="MUY14_40740"
     CDS             6759..9536
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020641768.1"
                     /locus_tag="MUY14_RS40740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_40740"
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_247017737.1"
                     /transl_table=11
                     /translation="MAPIRFRLLGPVQLLHGDQPVPIGGPGVRGLLALLALKPGKVVGL
                     DDIIDALWGHDPPATVRTIIHGNVSHLRRILRELDGPSILTTPPGYRLDVDPDRIDVHR
                     AHALLDRASVATPEVAAGLLAEALALWQGPALGGVPDSLRAPELEDLRRAVHGARVDAD
                     LELGRHAELIVELSPIVRAHPLAERTAGQLMRALYHAGRRGDALELYRTVSRATLGTMG
                     VEPGTELRWLHTRVLNDDLPALSRESADSRWALSRESADAAKPISQLPAAVPSLAGRGE
                     ELAWLDGLVSRAEAGETTIAVVTGTAGVGKSTLVVWWAHRAARKFPDGVLFASLRGFDP
                     HHPPLEPAELLTQFLLGLGVETAKIPELLHERVALYRSLIAGRRMLVLLDDARTAEQVR
                     PLLPPSARTMTVVTSRSRLDGLAVSNAARQRVLGTLAPEDAVQLIEELAGPASLNHALA
                     RLCGYLPLALRIAGARLSASAQRTAEELVDELGNERTRLAALQVEGADDGVRAAFDVSF
                     RGLPGEIAETLLQLGTVPGVLVGPHVMAAVAQIPVPEARRRLRALAAHNLIAETARDVF
                     VPHDLVWLYLRELVEQELGEKEREEALGWTVRYYQAAADRARRRLGPVADPLDFTGVLA
                     DDAMPPLTGFAEAHDWFAAEWPNLLAVLDAAAAAGRHDDVWRLARLGHSYRVACPLLDE
                     WTRMADLGVAAAEAAGDVAGQCWLRLARSEIALVFELPGFGLADAERAAELATGLADEW
                     LTTSADLHLGRALSRLGEHERAIERLGKAVAEAGDVTLRGQALSSCAQAEKRAGLLAEA
                     IAHQLAGLRIDRELGDDDRVVVSLDKLADLSLRVGDLEAAERYVWEAIDLAISREFVAR
                     EGSLRLTLGRVLRAGGDLDGAREQLALSVRLCRFGQKQAAPRAAGDRCYGPIRP"
     misc_feature    8340..8342
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(9633..9648)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.63"
                     /tool="antismash"
     misc_feature    9696..9714
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.76"
                     /tool="antismash"
ORIGIN
        1 gtgaccatgg tcgggggctc cagcctcgcg atctggatgg gcggggtcgc caccgagacc
       61 tcgcagctgc tccgggaatc gcgcagggac gccgaacccg ggcggtaccg caagctgctc
      121 gacgtcctgc gcgcgaccgt ttcactcgac gtcctgaccg ggaccagcgc cggcggggtc
      181 aacgccgcct gcctgggcct cgcggaagct ttccgttcca cgcccggggt tctgcgcgac
      241 acctggatca ccaccgcctc gctggagaac ctgatccggg acgcgcgcga gccggcgccg
      301 cggtcggtgc tcgacgggga ccgcgtgctg ctcggtgacc tcgaagcggt gctgcgcgag
      361 atcaccgcga acggcgtgcc gcccgccgag gagccggacg tcaccgtgct gctcaccggc
      421 acgatgatcg acggggagac cacccggttc gacgacgcgc tcgggaacct ggtacgggac
      481 accgaacacc ggatgctctt ccggttctgc ggtccactgt ggacgaacgg ggtggaggcg
      541 ccgctcgcgc tggcggcgcg ctccaccgcg tccttccccg gcgcgttcga actctcgcgg
      601 ctgccgatcg gcaccgcggg caccgaccgg ctgcaccccg acatgacgcc ctacaccgag
      661 ctgacccgct cgcactggct gaccgacggc ggggtgctgc tgaacaagcc gttgcggccg
      721 gcgctgcgcg agatcttcga acgcccgtcg cagacggacg tccggcggct gctgctctac
      781 gtcgtgccga ccggcgaacg ggacgccgac gccgtcgcgt gcgacccggc gaacccgccg
      841 ctgctgtcga acgcgctggc caaggtcgtc aacaccgtga tgagccagtc gatcagcgcc
      901 gagctggacg acctgacccg gcacaacgac gccgtgctgc gcgccaggga cacccgggtt
      961 tcgctggccg cgctggggtt gcgcggcggg cccggctgcc tggtcgacgc gcggatcgcc
     1021 accgcctacc tcgagcggcg gaccgccgaa gacgccgccg agctggtccg ggtcgccgcg
     1081 cggcggtacg cgctgtccga agcggacacc cccgaccgcg actgggcgtc cgggatgtcg
     1141 cggcagctgc gcgcgatcgc cgtcgccggg ctgagcagcg gcgttccggc cgggccgccg
     1201 gccgagcgcc tcgcggtcgc cgatctggtc gggtaccgga cgaccgcgct cgacgacgcc
     1261 gtcgcgaccg ggttgcagct ggtcaacgcc gggttccggc tccagccgga cgccgcgcgc
     1321 gcgaaagagc tcaacgacgc tcgcgcccag ctgcacgccg ctcgcgcgac cgccgggcgc
     1381 ggggtgcggc tcgggcagtg ggtggccgcg cacaccgggc ccggcgcgag cagcaccctc
     1441 gaggtgtgga tccgcgagct ggccgaggag tgggccgggc tgggccgctc ggccgccgtc
     1501 gccgaggcct ggccgaaggt ggtcgcgggc ttgcgcgcgg ccgcgccgac gctgcgggag
     1561 ctgaccgccg ccggggacga cgccggcacg gtgaccaccc tgctggactg gctcgagctg
     1621 cggcccgacg tggccgacga cgtggtccag gcccggctgg tggcactgca cgtggccacg
     1681 cgcgggctgc tcgcgcaggc gccgtcggtc gaccagcggg tcgacctggt gcaggtcagc
     1741 gccgattctc ggacgttact ggacatgacg cgcagccggt ccggggacaa gctgaccggg
     1801 atgcaggcga gctgcttcgg cgcgttctac aaggcgtcgt ggcgggccag cgactggatg
     1861 tggggccgcg tcgacggtgc cggctggctc gtccaaagcc tgctcgaccc gggccgcctc
     1921 gagacgctgc gggacgtcct cgggcgggag cggttccgcg acgagctcgt cgccgcgttc
     1981 ggcccggact ggcgcccgcc cggcgagcag gaccgctgca cgccggccga agcggcgcag
     2041 ctgcgcgacc agctgaccgc cgagctggcg ttcctcggcc tcgacgccga cctgaagccg
     2101 gccgagttcg gcgccgaacg gccggtcagc ctgccggtca cggcgatggt gctggcccgg
     2161 ccgcggcagg ccgagatcgc ggcggaggag ctgccggtcg tcacgctcac ctcgcgctgc
     2221 gacgccgacg accgccggga catcggcgac gcactggcga aggagcgggc cccgctcgac
     2281 gtcggcggcg cgcaggcgca gttccaggcc tgccgcgtct cggcggagcg gctcgccggc
     2341 gaacagggca ccgcgcggct cacccgcacg ctggtcgcgc tcggcgcggc gacggtgaac
     2401 gcggggacgg tcgcgttccg cctgcccggt ggctggccgc gcacggtcgc gggcatcctg
     2461 cgcacggtgg ccaggagcac ggcgaaggtc tcgcagggcg cgtcccggct cggcaccgcg
     2521 ggctcgctcg ccgccggcgt cctcgcgctg ctggccggcc tggtgctcgg caacgacggc
     2581 ggcgcggtgc tgcagtggat cggcctcccg gtgctggccg gcgcggtcgt ctacctggcg
     2641 acggcgctgc tcacgatggg ccacctcgcg cggaagctgt tcgccctgct cggggtgctg
     2701 gtggtggcgg cgctgctgct ggccgcgttc ctgcccccgc tggcccagcc gttcttcggc
     2761 tggctcggca gcgtcgtcgc gggctggcgg cgcggggagg gcgcggtgtg gtggctggtg
     2821 gtgtcgggtc tgctggtcct gcccgcggtg gtgatgccgc tgagcgggat cgtgcgacgg
     2881 ctgccgcacc ggcgggcggc gcggcagctg ccggtggccg cgtcgagcgg gcggcacagc
     2941 gggggcgacc ggcgccgccg cctgaccgtt cgcggtcgcg cggacacctg atccggcccg
     3001 tcgccttgcg atcacccggc tttgggttca tggtgcgact aggtcgccgg cacgcaccgg
     3061 cggcccaggc gcgcgtgtgc cccgggaccg tccacttcaa acatgtttgt gggactcctc
     3121 accgggtacc ccgcgtcgtc gcaggtcata cgggggtcgt gagctgcgtc gctggcgagg
     3181 cgaaaattct ctgttacgtt tcctgagtat gtccgtaacg cacttgatcg aatccccgac
     3241 caaggcggac ggcgcgaagc tgtggcgaat cgcgcgcgat tccgccaagc tcgatctcaa
     3301 ctcgccgtac gcatacatgc tgtggtgccg cgatttcgcc gagtcctcgg tggtcgcgcg
     3361 tgaagacggc gaggcggtcg gattcgtcat cgcctaccgc aggccggacg agcccgaggc
     3421 cgcgctcgtc tggcaggtcg cggtcgacgc gtcccagcgc ggaaagggcc tggccggggc
     3481 cctgctcgac gcgctctaca cgagtctcgt cgcggacggc gtgcgttacc tcgagaccac
     3541 catcaccccg gacaacgagg cgtccatccg gctcttcgcg tcgttcgcga agcggtggaa
     3601 cgccgcggtg gaaaccagtg tgctgttcgc cggggaggat ttccccgaag ccgggcacct
     3661 ggccgaagag ctttaccgca tcggtccgct cacgccacgc aaagatccgg gcgagtagga
     3721 gaaaagaaac gtcatgagca tcttcgaaga gctcgaatcc gaagtacgca gctacagccg
     3781 cggctggccg gtcgtgttcg accgcgcgca gggcagccac ctctacgacg agaacggcaa
     3841 ggcctacctg gacttcttcg ccggggccgg cgcgctgaac tacgggcaca acaacccgcg
     3901 gctcaagcag gcgctgatcg actacatcca gcgcgacggc gtcacccacg cgctggacat
     3961 gttcaccgtg gccaagcggg acttcctgca gaccttccgc gacaagatcc tcgacccgcg
     4021 gaacatgaac tacaaggtcg tgttcccggg cccgggtggc gcgaacgccg tcgaggccgc
     4081 gctgaagctg gcgcgcaagg tgaccggcaa ggaatcggtc atcaacttca ccaacgcctt
     4141 ccacggcatg acgctgggcg cgttgtcggt caccggcaac tcgatgaagc gcggtgccgc
     4201 gggcatcccg ctggtgcacg ccaccccgat gccctacgac gcctacttcg acggcgcgat
     4261 gccggacttc ctgtacttcg agaagctcct cgaagactcc ggcagcgggc tcaacgagcc
     4321 ggccgcggtg atcgtcgagg gcgtgcaggg cgagggcggc atcaacgccg cgcgcctgga
     4381 gtggctgaag ggtctcgacg acctctgcaa gcgccacaac atcctgctga tcctcgacga
     4441 cgtccagatg ggctgcggcc gcaccgggcc gttcttcagc ttcgaggacg ccgggatctc
     4501 cccggacatc gtctgcctgt cgaagtccat cggcggctac ggcatcccga tggcgctgac
     4561 gctgatcaag ccggagctcg acgtctggga gccgggcgag cacaacggca ccttccgcgg
     4621 catcagcccc gcgttcgtca ccgcgaccga ggcgatcaac gcctactgga gcgacgacga
     4681 gctcgagaag tccaccaagg cgaagggtga gcgcatcgcc gccgcgttct cgggcatcgt
     4741 cgaggcgtac ccggacgcga acctggtcgc caagggccgc ggcctcgcgc gcggcctggg
     4801 gttcgagaac ggagacctcg ccggccgcgt ctgcgcggaa gcgtttgcgc gcggcctgct
     4861 gatggaaacc tccggtcccg acggcgaagt catgaagctc ctgccgccgc tgaccctgac
     4921 cgacgacgaa ctgacccagg gcctgtcgat catcgacgag tccatcaagg ccgttctcaa
     4981 gaagtagagg agccacgccc gtgctcgttc gcacgctcga cgaaatcacc gacaccgacg
     5041 ccgacatcaa gaccgagaac tggcgcagca agcggatcat cctcgccaag gagggtgtcg
     5101 gcttctcggt gcacgagacc acgctgtacg cgggaacggt gaacgacttc tggtacgcca
     5161 accacatcga ggcggtgttc atcacctccg gtgaaggcga gatcgaagac ctggccaccg
     5221 gcaaggtgta cgagctcaag ccgggaacgc tgtacctgct caacgaccac gacaagcacc
     5281 aggtccggcc gaagaccgag atcaagtgcg tctgcgtgtt caaccccccg gtgaccggcc
     5341 gcgaggtgca cgacgaaaac ggtgtgtacc cgctgatcac cgaggacgcg taaaacaccg
     5401 gagaggaaac tggaggcgaa gcccgtgacg ctgatggaca cccgggtcga cgacacctac
     5461 ccgacccgga tcaccggtac tccggcgcac ctgccgcgcg cgcaccccac ggtgtggggt
     5521 accgaagccg acggcccgat cgacgccgcc acgctggcga accacgagac caacggctac
     5581 accgtggtcg aggacatgct ctccgtcggg gaggtccagg cgtactggca ggagctggtc
     5641 cggctgtctt ccgacgcgga gctcgccagg gacgagcgcg tgatcaccga agcgaagacc
     5701 ggtgaagtcc ggtcgatctt cgacgtccac gagatctccg ccctgatcgc cgaactggtg
     5761 cgcgacccgc gcgtgctgga ccgggcccgg cagctgctcg gctccgaggt gtacatccac
     5821 cagagccgcg tcaactacat gccggggttc aagggcacgg gcttctactg gcactcggac
     5881 ttcgaaacct ggcacgcgga ggacggcatg ccgtccccgc gcgcggtcag ctgctccatc
     5941 gcgctgaccg acaactaccc gttcaacggc ggcctgatga tcatgccggg ctcgcaccgg
     6001 acgttcgtcc agtgcgccgg cgagacgccg gaggcgaact acaaggcctc gctcaaggac
     6061 cagcgggtgg gcgtgccgaa cgaggacgac atcacccgga tggcggccga gcatggcatc
     6121 gaccagttca ccggccaggc gggctcggcc ctgtggttcg actcgaacat catgcacggc
     6181 tccgggaaca acatcacccc gtacccgcgc tcgaacatct tcctggtgtt caacagcgtg
     6241 gagaacgcgt tgcaggagcc gttcgcggcg agcgccccga ggccggcctt catcgccggc
     6301 cgcgactcga cgccgatctc gcggtagcag tgtcgtaccg gggcttcgcc acccggatcc
     6361 cccgaaaagc gtggggcccg ccggtgaccg gcccggtgcg ctttgttacc gtgtcgccac
     6421 cgcgcgggcg tgtggtccgc gcaacagacg tcttcgacgg cgaacggcgg cgggtgcttc
     6481 gctcggggtg agcacacccg cccgatcgcc ggcgaagacg acgggcggac cgcgccccct
     6541 ggcggcccgg ccggaggttt cacacgaccc cgtaccaacg gatcgggacc gttggtacgg
     6601 ggtcgttcca cgtccggggg atgtcgcagg acgtggacac ccgcgctgcg gaatccgcag
     6661 aaccgccaaa actcctggta ccggacctgc tcgacgacct gccaccacgg taggttgatt
     6721 tccgcggcaa ggcggaaccc ggggaggtgg gcgcgagcat ggccccgatc cggttccggc
     6781 tgctcgggcc cgtgcagctc ctccacggcg accagcccgt cccgatcggc ggccccggtg
     6841 tccgcggcct gctcgcgctg ctggccctca agcccgggaa ggtcgtcggg ctcgacgaca
     6901 tcatcgacgc gctctggggc cacgacccgc ccgcgacggt gcgcacgatc atccacggca
     6961 acgtctcgca cctgcggcgg atcctgcggg agctcgacgg ccccagcatc ctcaccaccc
     7021 cgccgggcta ccggctcgac gtcgaccccg accggatcga cgtgcaccgc gcgcacgcgc
     7081 tgctggaccg ggcttcggtg gccacccccg aggtcgcggc cgggctgctg gccgaggcgc
     7141 tcgcgctgtg gcaggggccc gcgctcggcg gcgtgcccga ctcgctgcgg gcccccgagc
     7201 tggaggacct ccgccgcgcc gtgcacggcg cccgcgtcga cgccgacctc gaactcggcc
     7261 gccacgccga gctgatcgtc gagctcagcc ccatcgtgcg ggcccacccg ctggccgagc
     7321 ggacggcggg gcagctgatg cgggcgctct accacgccgg ccgccgcggg gacgcgctcg
     7381 agctgtaccg gaccgtctcg cgcgccaccc tcggcaccat gggcgtcgag cccggcaccg
     7441 agctgcgctg gctgcacacg cgggtgctca acgacgacct ccccgcactt tcacgtgaaa
     7501 gtgcggactc caggtgggca ctttcacgtg aaagtgcgga cgccgcgaag ccgatttcgc
     7561 agctgcccgc cgccgttccg agcctggccg ggcgcgggga agagctggcc tggctcgacg
     7621 gcctggtctc ccgcgccgag gcgggcgaga ccacgatcgc cgtggtcacc ggcaccgccg
     7681 gcgtcggcaa gagcacgctc gtcgtgtggt gggcgcaccg cgccgcgcgg aagttcccgg
     7741 acggggtcct gttcgcgtcg ctgcgcggct tcgacccgca ccacccgccg ctggagcccg
     7801 ccgagctgct cacccagttc ctgctcggcc tcggcgtcga aaccgcgaag atccccgagc
     7861 tgctccacga acgcgtcgcg ctgtaccggt cgctgatcgc cgggcggcgg atgctggtgc
     7921 tgctcgacga cgcccgcacc gccgagcagg tgcgcccgct gctgccgccg agcgcgcgga
     7981 cgatgacggt ggtgaccagc cgttcgcgcc tcgacgggct cgccgtgtcg aacgcggccc
     8041 ggcagcgcgt gctcggcacg ctcgcgcccg aggacgccgt gcagctgatc gaggagctcg
     8101 ccgggccggc gagtctcaac cacgccctcg cgcggctctg cggctacctg ccgctcgcgc
     8161 tgcggatcgc gggcgcgcgg ctttcggcga gcgcgcagcg gaccgccgag gagctggtcg
     8221 acgagctcgg caacgagcgg acgcggctgg cggccctgca ggtcgagggc gccgacgacg
     8281 gcgtgcgcgc cgcgttcgac gtctccttcc gcggcctgcc cggcgagatc gccgagacgt
     8341 tactgcagct gggaacggtg cccggggtgc tggtcgggcc gcacgtgatg gccgcggtcg
     8401 cgcagatccc ggtgcccgaa gcccggcggc ggctgcgcgc gctggccgcg cacaacctca
     8461 tcgccgagac cgcgcgggac gtcttcgtcc cgcacgacct cgtctggctc tacctgcggg
     8521 agctcgtgga gcaggagctg ggcgagaagg agcgcgaaga agcgctcggc tggacggtcc
     8581 gctactacca ggccgccgcc gaccgggcgc ggcgccggct ggggccggtc gcggacccgc
     8641 tcgacttcac cggggtgctc gccgacgacg cgatgccccc gctgaccggc ttcgccgagg
     8701 cgcacgactg gttcgcggcg gagtggccga acctcctcgc ggtcctcgac gccgcggccg
     8761 ccgcgggccg ccacgacgac gtctggcggc tggcgcggct cgggcacagc taccgcgtcg
     8821 cctgcccgct gctggacgag tggacgcgga tggccgacct cggcgtcgcc gccgccgaag
     8881 ccgcgggcga cgtcgccggg cagtgctggc tgcggctcgc gcggtccgaa atcgcgttgg
     8941 tcttcgagct gccggggttc ggcctagcgg acgccgagcg ggcggcggag ctggccaccg
     9001 gcctcgccga cgagtggctg acgacgtcgg ccgacctgca cctcggccgc gcgctcagcc
     9061 ggctcggcga gcacgagcgg gccatcgagc ggctcggcaa ggccgtcgcg gaggccggcg
     9121 acgtcacgct gcgcgggcag gcgctcagca gctgcgcgca ggcggagaag cgggcgggcc
     9181 tgctcgccga ggccatcgcc caccagctcg ccgggctgcg catcgaccgc gaactcggcg
     9241 acgacgaccg ggtcgtcgtc tcgctcgaca agctggccga cctcagcctg cgggtgggcg
     9301 acctcgaggc ggccgagcgg tacgtctggg aggcgatcga cctcgcgatc agccgcgagt
     9361 tcgtcgcgcg cgagggttcg ctgcggctga cgctgggccg cgtgctgcgc gccggtggcg
     9421 acctcgacgg cgcgcgcgaa caactcgcgc tgtcggtgcg cctttgccga ttcggccaga
     9481 agcaggccgc cccacgagcg gcaggcgacc ggtgctatgg cccgattcgg ccgtgactgt
     9541 ggccggatcg gcaaacaacg gcactgtcga gcgggtgcac ccgaagctcg acggcgaggt
     9601 gcgcaccgaa ctcgccgaac tgcagtgaag attcgcccgc gctttcgaac tgcgacaact
     9661 cggtgtcggc tcggcgcatt catggcgatg attcggttgc gaacgggtca cggccgggtt
     9721 cgcaccgtga atccttagtg gattcttagt gggcgcgggc ggcgtccgcg taggttcggc
     9781 ggtgccggct gagccctagg ggaggggcag gctgagacgc cggcgccggg ccccgcgcgg
     9841 gaaaccgcgg cggggccggg agccgccagg agcggctcgt ggcgacccgg ccggccgagg
     9901 tgggggtagg ccggccggtc gccaggcagg ggtcagccca cgttggcggt gcacagcgcg
     9961 atggtcgtcc cgctgttttc cgcgaccttc ttgccgtcga ccagaatggc gcacttgatg
    10021 tcgttgctcg cgctcgaatt gctggtgtcc gcggtgagcg tcaacaagta cgaaccattg
    10081 ttgtaggaag cctcgccctg ccacgcgtcg gtgctcgccg gcgcgttttc ggtgcgctgg
    10141 tcgctgaggc tgccgtagcg gacggtcgcg ccgcccgccg aggtgacctc gaagaccacc
    10201 gcgtgcttgt cgcccgcgac cgcgggcagg tgcagcccgg tctcgccgcc gccgaggaag
    10261 gtcgagtaga gcagcacgcc cgccgtcgac agcagtgccg cgaccgaaac gacggtggcg
    10321 gtgatcgcga ccccgcggtc gcccgtggtg cccttcttga cctggaccag cccggcgatc
    10381 gcgaggatca gcc
//
