LOCUS       NZ_CP095376            12307 bp    DNA     circular CON 06-NOV-2024
DEFINITION  Amycolatopsis sp. FBCC-B4732 chromosome, complete genome.
ACCESSION   NZ_CP095376
VERSION     NZ_CP095376.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis sp. FBCC-B4732
  ORGANISM  Amycolatopsis sp. FBCC-B4732
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 10190959)
  AUTHORS   Choi,A.
  TITLE     Complete Genome Sequence of Amycolatopsis pretoriensis strain
            FBCC-B4732
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10190959)
  AUTHORS   Choi,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2022) Bioresources Collection & Research Team,
            Nakdonggang National Institute of Biological Resources, Donam 2-gil,
            Sangju-si, Gyeongsangbuk-do 37242, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 75.1x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023008405.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 10:39:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,364
            CDSs (total)                      :: 9,298
            Genes (coding)                    :: 9,177
            CDSs (with protein)               :: 9,177
            Genes (RNA)                       :: 66
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 50 of 121
            Pseudo Genes (incomplete)         :: 81 of 121
            Pseudo Genes (internal stop)      :: 14 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:14:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3613768
            Orig. end                         :: 3626075
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095376.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from NNIBR.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12307
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="22"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12307
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12307
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7307](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7307
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    86..103
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 23.88"
                     /tool="antismash"
     misc_feature    complement(104..118)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
     misc_feature    complement(170..184)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.46"
                     /tool="antismash"
     gene            780..1928
                     /locus_tag="MUY14_RS15505"
                     /old_locus_tag="MUY14_15505"
     CDS             780..1928
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1189: L-carnitine dehydratase/bile acid-inducible"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005449727.1"
                     /locus_tag="MUY14_RS15505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_15505"
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="WP_247023710.1"
                     /transl_table=11
                     /translation="MSEQSGPLAGLRVVKVERPGTGDELRRWRLHDGDTSLLFRTLGRN
                     KRSVTVDLRRPEGQDIVRALAARSDVLLENFRPGTLERWGLGPGELRALNPRLVVVRVS
                     GYGQTGPYRDRPGFGGVAEAIGGLRALTGYPGQAPTRVGVSLADSVAGLYAVIGALMGL
                     LRREREPGARPGEVVDVALYEAVYSLMESLLPDFDAHGVVRGPAGSALPGVAPSNTYRC
                     ADGKYIVVSGNGDAIFRRLAHAIGRADLAADPRPADNAGRVRHTGLLDDAIGAWAATLT
                     HEAAGAALLAASVPSGPILTAADIAKDPHFEARGMHERHRVRVGAGREAEVTFPGIVPA
                     LAERPGRTRSLGPDLGEHTEAVLAELGITGDAVAGLREKGVI"
     gene            1930..2832
                     /locus_tag="MUY14_RS15510"
                     /old_locus_tag="MUY14_15510"
     CDS             1930..2832
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HMGL-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1271: 2-isopropylmalate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005464562.1"
                     /locus_tag="MUY14_RS15510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_15510"
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="WP_247023711.1"
                     /sec_met_domain="HMGL-like (E-value: 2e-39, bitscore:
                     134.8, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEVLITEVVLRDGLQDEPVTVPAHEKVRLARGLVDAGVRALEVG
                     SFVSARRVPQLADTGEVLRALLPDTPACLHTLVFTERGARQAIDAGAKSVRLVVSAGDG
                     HSTANAGVPAAAALDRLAGCAELLTGAGVALEASVATAFACPFDGDTEPDRTADVAAHL
                     GRLGARVVHLADTIGAARPSRLRAVVSAVPDELPDVPLGLHLHNTYGRAAANAWEGLSL
                     GIRRFDAALGGIGGCPFAPGASGNVGTDDLVDFFHGEGIATGIDVAKLAEVRDQVGLAV
                     GHRLGSALAAISAVPAPLR"
     misc_feature    complement(2838..2853)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 20.92"
                     /tool="antismash"
     misc_feature    2912..2927
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.15"
                     /tool="antismash"
     misc_feature    complement(2962..2979)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.23"
                     /tool="antismash"
     gene            3152..4582
                     /locus_tag="MUY14_RS15515"
                     /old_locus_tag="MUY14_15515"
     CDS             3152..4582
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020639110.1"
                     /locus_tag="MUY14_RS15515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_15515"
                     /product="DUF1996 domain-containing protein"
                     /protein_id="WP_247023712.1"
                     /transl_table=11
                     /translation="MPSSTQGRHRLSRRTKIATGGLALAIAVGGIVVATTTGNTGEASA
                     DEANPAFFVDILKVKPNQFDPRPVYGAATGTFTVDCGRNENQHFNPDNFIAQPGVRNGA
                     QHLHDYVGNLSTNADSTNKTLAKAGTTCRNGDKSAYFWPVVRIDTGEEDKNEPAKAPDG
                     DRAQAAQESKTVQVACPDVASKLPDIPDQAMDEVDSNLDQLDSQADEANKKVAAAQGQG
                     GENLVQNSIIGPLNDRRKAIIDRILTAIGRFAPKPGGIGGLPCGTKPGKDTGSPTPPPS
                     STAGGALPGQDDNNELPGNDGKILRPQKVQITFRGSPVGKVVAMPRFLRVLYGDAKVST
                     NGTANARASWTCSGFENRVTEKYPICPENSKVKRVHTFPSCWDGQNTDSANHRTHIVFP
                     DQNGRCAKGFKAVPQLQISLTYDIPHDIQVKKQYKVDAFPQEKHNPFSDHDDFANVMSQ
                     RIMNGLVNCVNRGRTCRA"
     misc_feature    complement(4689..4704)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.73"
                     /tool="antismash"
     gene            4793..4927
                     /locus_tag="MUY14_RS46890"
     CDS             4793..4927
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004561011.1"
                     /locus_tag="MUY14_RS46890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_255482016.1"
                     /transl_table=11
                     /translation="MISSFERPSAVVVHNRAVVHNRAVVHNRAVVHNRAVVHNRASAK"
     gene            5001..7307
                     /locus_tag="MUY14_RS15520"
                     /old_locus_tag="MUY14_15520"
     CDS             5001..7307
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03604"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226216.1"
                     /locus_tag="MUY14_RS15520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_15520"
                     /product="YcaO-like family protein"
                     /protein_id="WP_247023713.1"
                     /sec_met_domain="TIGR03604 (E-value: 5.3e-75, bitscore:
                     251.6, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="YcaO (E-value: 1.7e-55, bitscore: 188.2,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPSDDIRLQLTGVELHDLEPELCLLSTPNGGQYSITAPVGEFRAW
                     LSRCDGTRTRAELLAGMSPDHAEVLDVLEADGCLHPATGDDGARRLAATTVLVTGAAEL
                     TGPLAEVLGATGYGSVRPLAEAALLVNPAEVLRTAGDGPARSADPADPAEILGSAAYNA
                     VPPLVEAAFPLAPADTVLVAAYTHPAYRELSALDAFCTEHGVRFFPFRCERGQGIAGPA
                     VEPGFGPDFADVLARRRSAALDARVPDAFAAAEAPPGPRFRRTDARWLVSALAVQLERW
                     IAGEPAETTTGELELDPVRLTALPRPVLPVPDRPRPVRDQGLRPDLLVDDRTGIVTAVH
                     ELPAAPGMPARLKVCAVDVADMRRVVDWPNDRQAFGTSWHDFEPARESAIGEAVERYCG
                     SWLPPERELRHGSYDQLRRAGVPALDPRRLNLYSARQHRTPGFPFAPLTTDAECAWVEA
                     FSHTTKEPVWVPACLVSQAPEPGGARFTDPLIAGLAGGTSAEHAVTSGLEEVLERDTTM
                     VWWANAPRLRALPVPPEVRALVADTTETYEVTLIPLDNEFAVPVVAAAVFDRARRWLSI
                     GFATRPDALEAAKKALAEGFTLQHTCLALDDERELAAMRDDLPHLGNLKPYRADRRYLD
                     SYRADFGDVVDLLCQQQVYLDPRAAAHVAPWVRDLPERSWEGLPALGERTLKTYQDRVE
                     ARGFEVLSVDLTTRDVAAAGFHAAHTLVPGLVANFPAGLPYWGDGRVRRAAVDLGWRAH
                     PLPEARLNTFPLPHA"
     gene            7318..8538
                     /locus_tag="MUY14_RS15525"
                     /old_locus_tag="MUY14_15525"
     CDS             7318..8538
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1202: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226215.1"
                     /locus_tag="MUY14_RS15525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_15525"
                     /product="MFS transporter"
                     /protein_id="WP_247023714.1"
                     /transl_table=11
                     /translation="MAVTALLGLPRTRGRLPLVAAQGVDALGTGLFLPFAVVYFHAAKG
                     LPLTAVGAALSLAALLALPAGLCAGPLVDRFGPRRVVVAANLLRVLTFTGYVFAGSPPV
                     LVVLAALTFWGEGLFWPASGALVAQVADDGQRARWYAMERTLRNVGIGLGGLAGSVLVV
                     VGGYAAIVVLNAVSFLVAAVLVATWRGRAGEAPARTTAPRGGYRAVLADRAFRRVLVTV
                     FVFALCDLALTVLLSAYVLDALRLPAWQPGVLFALNTVLVVLAQTVVAGRVERFRRGRT
                     LQVAAVVWAGAFLLYAAVPLVTALPAFAVLTLATAVFTVAELLQAPTSSALTVALAPAH
                     LRGRYLGLEEVLWGAARVLAPVTFTALLAGGPQLPWLVLAGCCLLAVAVLTGLKAVPEP
                     VRETANA"
     gene            8531..9001
                     /locus_tag="MUY14_RS15530"
                     /old_locus_tag="MUY14_15530"
     CDS             8531..9001
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226214.1"
                     /locus_tag="MUY14_RS15530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_15530"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_247023715.1"
                     /transl_table=11
                     /translation="MPDWTAVTGLYHQVYREPPYGESEADVARFRETLAQHAELPGFAL
                     TAVHSGDVLDGFAYGVGREAGWWPSPAAAAPPPWLAGRPLFYVYELAVRPGRRGRGHGR
                     DLLDRLLRDRAEPCALLAAATAAPAHALYRRWGWEAVGVFRPGVSELLARRL"
     gene            complement(8961..10184)
                     /locus_tag="MUY14_RS15535"
                     /old_locus_tag="MUY14_15535"
     CDS             complement(8961..10184)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680275.1"
                     /locus_tag="MUY14_RS15535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_15535"
                     /product="MFS transporter"
                     /protein_id="WP_247023716.1"
                     /transl_table=11
                     /translation="MHRTFWRLWTAAGLSSLADGVLKVALPLVAVGYTREPALVAGLAF
                     AFSVPWLLFALPAGALVDRLDRRRAMLGANVLRGALVAVVAVLTAYGGGSIWALYVVAL
                     GAGTAETLYDTAAQSIVPQVVGRADLARANGRVYAVEQTANEFAGPPLAGVLVTAGVLA
                     AFVTPVVLWAVAVAALLFVRGSYRIPRGDRPATLRADIAEGLRFLARHKVLRTFSLMVG
                     TFNFATGATFAVLVLYAVGPGSAMGLSGPAFGLLTATTAAGGLAGSLLAETVERRLGRV
                     RALRVSWLAGGLTVGVLAVTADPYVVGAAFFAGGAGILVSNVVMVSLRQRITPDRLLGR
                     LTSSHRLVAWGTKSLGALAGGALAQAFGLHVVFVVMTVLAFSVVGGLFVVTEDALRAAE
                     PAVPRHPA"
     gene            10260..10790
                     /locus_tag="MUY14_RS15540"
                     /old_locus_tag="MUY14_15540"
     CDS             10260..10790
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647356.1"
                     /locus_tag="MUY14_RS15540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_15540"
                     /product="transcriptional regulator"
                     /protein_id="WP_247023717.1"
                     /transl_table=11
                     /translation="MNEPRRRRPATPEEAKALGHPLRLRIMRLCLVDELTNKQLADRLD
                     RDPGTVLHHVRQLVSAGLLHPAPVRTGPSGALEKPYRADNETWWLDGPLATADAETRFA
                     PIEIFQEELHAAGPDSVAVHEKFLLHLSPDEVEELDRRILAVLDEYVATDHERPDRPAI
                     GGIFVLHHLKPGT"
     gene            complement(10841..11599)
                     /locus_tag="MUY14_RS15545"
                     /old_locus_tag="MUY14_15545"
     CDS             complement(10841..11599)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226212.1"
                     /locus_tag="MUY14_RS15545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_15545"
                     /product="SH3 domain-containing protein"
                     /protein_id="WP_247023718.1"
                     /transl_table=11
                     /translation="MRIRIGKRVAGVAGIAAAIALAGATPALAATGTVHTDSGIALTVR
                     STPSTGGSAVGTIADGTAVTISCQTNGSTVDGKYGTSDIWDKVGDGYISDAYVYTGSDG
                     RVAPDCAGSTLSCTTAGTGNPKTCAQAVAYAKTRVHTNDLDSYEGWCDRINAQNYGWTA
                     SGSETAYIHWTQIPASFKHPGDTQVPAGGLAFFKSSGAGHTMISIGGGKFLSNDINGYG
                     SYTETTIAQIKSKWGQTYLGWSQPWFKVNH"
     misc_feature    complement(11585..11600)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.5"
                     /tool="antismash"
     misc_feature    11855..11873
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.98"
                     /tool="antismash"
ORIGIN
        1 cgccgcgccg acctcgccgt tcagcccgaa caccggggcg ctcaacgacg ccgcgcccag
       61 gtcacgttcc tcgaagctca ccgcgtaccc cgtcgagcgg atctggccga gctgctcggt
      121 cagcaggtcg atgtccacca ccgtgcgcgg ggtgaaccgg gccaggccgt ggccgatcgc
      181 gtccttgatc gccagtttgt cccaggccag gaagaccttg cccgccgaac ccgcgtgcag
      241 cggcatcacc atgccgacca cgaacagccg gaccaccggg tggcgggact cggccagcga
      301 aatcaccgtg cggtaggcgc cgtcgcggac gtacaggcac gccgtctcgc ccgtttcgtc
      361 gcgcagctgc tgcagcaccg ggcgggtcag ccggaccagg tccaggccga acgtgccggg
      421 cgccgcccag cggaccaggc cgaggccgac gcggtaggtg tcgtcgtagc ggtcgaggaa
      481 gccttcgcgc accaggttgt gcaccagccg ctggcacgtg ctcgccggca gcccggtgga
      541 ccgcgtgatc tgctgcagcg tcagctccgg gtcgtcgatc gtgaacgact cgaggatctg
      601 cttgaacttg cgcagcacca gcaccggcgt cgtcccgccg ccactgcgct cttcctctgc
      661 catcgccccg ccccgtgccc gtggtgtgcc gcacagtcta gcgacgctcg cccccgttga
      721 ctccacgaca cacgcccgta ttctgagttg acagctcaca tagtagtttg tggtcgggca
      781 tgagtgaaca atccgggccc ctcgcgggcc tccgggtggt caaggtcgag cgccccggca
      841 ccggcgacga gctccgccgc tggcggctgc acgacggcga cacctcgctg ctcttccgca
      901 ccctcggccg caacaagcgc tcggtgaccg tcgacctccg ccgtcccgaa ggccaggaca
      961 tcgtgcgggc gctggccgcg cgcagcgacg tcctgctcga gaacttccgg cccggcacgc
     1021 tcgaacgctg ggggctcgga cccggcgagc tccgcgcgct caacccgcgg ctggtcgtcg
     1081 tgcgcgtctc cggctacggc cagaccggcc cgtaccgcga ccggcccggc ttcggcggtg
     1141 tcgccgaagc gatcggcggg ctgcgcgcgc tcaccggcta ccccggccag gcgcccaccc
     1201 gcgtcggggt cagcctcgcc gactccgtcg ccggtctcta cgcggtgatc ggcgcgctca
     1261 tgggcttgct ccgccgtgaa cgcgagcccg gcgcccgccc cggcgaggtc gtcgacgtcg
     1321 cgctctacga agccgtctat tccctgatgg agtcgctgct cccggacttc gacgcgcacg
     1381 gcgtcgtgcg cggcccggcc ggctccgcgc tgcccggggt ggcgccgtcc aacacctacc
     1441 gctgtgccga cggcaagtac atcgtggtca gcggcaacgg cgacgcgatc ttccgccggc
     1501 tcgcgcacgc catcggccgc gcggacctcg cggccgaccc gcggccggcg gacaacgccg
     1561 ggcgcgtccg ccacaccggc ctgctcgacg acgcgatcgg cgcgtgggcg gccaccctca
     1621 cccacgaagc cgccggagcc gcgctgctgg ccgcttccgt gccgagcggc ccgatcctca
     1681 ccgccgcgga catcgcgaag gacccgcatt tcgaagcccg cggcatgcac gaacgccacc
     1741 gcgtgcgcgt cggcgcgggc cgcgaagccg aagtcacctt ccctggcatc gtccccgcgc
     1801 tggccgaacg gcccgggcgc acccgctcgc tcgggccgga cctgggcgaa cacaccgaag
     1861 ccgtgctcgc cgagctcggc atcaccggcg acgccgtcgc cggcctccgc gaaaagggag
     1921 tgatctgacg tgaccgaagt cctcatcacc gaggtcgtgc tgcgcgacgg gctgcaggac
     1981 gagcccgtca ccgtgccggc gcacgaaaag gtccggctcg cccgcggcct ggtcgacgcc
     2041 ggcgtccgcg cgctcgaggt cggctccttc gtctccgcgc gccgcgtacc gcagctggcc
     2101 gacaccggcg aggtgctccg cgcgctgctg ccggacaccc ccgcgtgcct gcacaccctc
     2161 gtgttcaccg aacgcggggc gcggcaggcg atcgacgccg gcgccaagtc ggtgcggctg
     2221 gtcgtctccg ccggcgacgg gcacagcacc gcgaacgccg gggtcccggc ggccgcggcg
     2281 ctcgaccggc tggccgggtg cgccgaactg ctcaccggcg cgggggtcgc gctcgaagcg
     2341 tcggtcgcga ccgcgttcgc ctgcccgttc gacggcgaca ccgagccgga ccggaccgcc
     2401 gacgtggccg cgcacctcgg ccggctcggc gcacgggtcg tccacctcgc cgacacgatc
     2461 ggcgccgccc ggccgagccg gctgcgcgcg gtcgtctcgg cggtgcccga cgaactgccg
     2521 gacgtcccgc tcggcctgca cctgcacaac acctacgggc gcgcggcggc caacgcgtgg
     2581 gaagggctct cgctcgggat ccgccggttc gacgccgcgc tgggcgggat cggcggctgc
     2641 ccgttcgccc cgggcgcgtc cggcaacgtc ggcacggacg acctcgtcga cttcttccac
     2701 ggcgaaggaa tcgcgaccgg aatcgacgtc gcgaaactcg ccgaggtgcg cgatcaggtc
     2761 ggcctcgcgg tcggccaccg gctcggctcg gcgctggccg ccatttccgc ggtgcccgcg
     2821 ccgctgcgct gatcccgggt tcgcgcaagg ctttccggcc gggcccagtc gccgggcctc
     2881 acctggggat acggacgggg gttcagttcg gttcaaactt ttttcggtgc ttgcccggtg
     2941 tcaaaggccc gtgtaacttc cataacggaa cgataaccag ccggttgacg gcgcggccac
     3001 gggcaggtga gcgcgcaccc actgatgtcg ggtcagtgga ccagccggaa agccgttcct
     3061 tgccgacgca ttcctgatgg gcacgagcct gggctcgtac ccgttcagtg tcgcgcgttc
     3121 ccgccagccc gacaccccgg aggatttccc catgcccagc agcacccaag gacggcaccg
     3181 cctgtcccgc agaaccaaga tcgcgaccgg cggcctcgcc ctcgcgatcg cggtcggcgg
     3241 catcgtcgtc gcgacgacca ccggaaacac cggcgaagcc agcgccgacg aagcgaaccc
     3301 cgcgttcttc gtcgacatcc tgaaggtgaa gccgaaccag ttcgacccgc ggccggtcta
     3361 cggcgccgcg acgggcacgt tcaccgtcga ctgcggccgc aacgagaacc agcacttcaa
     3421 cccggacaac ttcatcgcgc agcccggcgt ccgcaacggc gcccagcacc tgcacgacta
     3481 cgtcggcaac ctctccacca acgccgactc caccaacaag accctcgcga aggccggcac
     3541 cacctgccgc aacggcgaca agtccgccta cttctggccc gtcgtccgga tcgacaccgg
     3601 cgaagaggac aagaacgagc ccgcgaaggc gcccgacggc gaccgcgcgc aggccgccca
     3661 ggagtcgaag acggtccagg tcgcctgccc ggacgtcgcg agcaagctgc cggacatccc
     3721 cgaccaggcg atggacgaag tggacagcaa cctcgaccag ctcgactccc aggccgacga
     3781 ggcgaacaag aaggtcgcgg cggcgcaggg ccagggcggc gagaacctcg tccagaacag
     3841 catcatcggc ccgctcaacg accgccgcaa ggcgatcatc gaccggatcc tcaccgcgat
     3901 cggccggttc gcgccgaagc cgggcggcat cggcggcctg ccgtgcggga cgaagcccgg
     3961 caaggacacc ggatcgccga cgccgccgcc gagcagcacc gcgggcggtg cgctgcccgg
     4021 gcaggacgac aacaacgagc tgcccggcaa cgacggcaag atcctgcggc cgcagaaggt
     4081 ccagatcacc ttccgcggca gcccggtggg caaggtcgtg gccatgccgc ggttcctgcg
     4141 cgtgctctac ggcgacgcga aggtctcgac caacggcacc gccaacgccc gcgccagctg
     4201 gacgtgcagc gggttcgaga accgcgtcac cgagaagtac ccgatctgtc cggagaacag
     4261 caaggtcaag cgcgtgcaca cgttcccgag ctgctgggac gggcagaaca ccgacagcgc
     4321 gaaccaccgc acgcacatcg tgttcccgga ccagaacggc cggtgcgcca agggcttcaa
     4381 ggcggtgccg cagctgcaga tctcgctgac ctacgacatc ccgcacgaca tccaggtcaa
     4441 gaagcagtac aaagtggacg ctttcccgca ggagaagcac aacccgttct ccgaccacga
     4501 cgacttcgcg aacgtgatgt cacagcggat catgaacggg ctggtcaact gcgtgaaccg
     4561 cggccggacc tgccgggcgt gagctgagcc ggaccgaagg cccccaccga gcgccggtgg
     4621 gggccttcgt cgtgcacggg tcatgtccgg accgtgacga acgcatgggc gcgggcgtgc
     4681 ggatcgcagt ccagaagacg tcaggacgca cggcgccgcc cgaaactggt tcccgtcacc
     4741 ccgccccggt gggcccgccg ggggacatcc acttcacccc gcaggaggaa ccatgatctc
     4801 gagcttcgag cgtccctcgg ccgtcgtcgt ccacaaccgc gcggtcgtgc acaaccgggc
     4861 cgtcgtccac aaccgcgccg tggtccacaa ccgcgccgtc gtgcacaacc gcgcgtccgc
     4921 caagtagtcc cggcccagcc gtagacgtgc cgcggccctt ccccgggccg cggcacggcc
     4981 ccgccgacga ggggatcgcc atgccttccg acgacatccg cctgcagctg accggcgtcg
     5041 agctccacga cctggagccg gagctgtgcc tgctcagcac cccgaacggc ggccagtact
     5101 cgatcaccgc gccggtgggc gagttccgcg cgtggctgag ccgctgcgac ggcacccgga
     5161 cgcgcgccga gctgctcgcg gggatgagcc cggaccacgc ggaggtgctc gacgtcctcg
     5221 aagcggacgg ctgcctgcac ccggccaccg gcgacgacgg cgcccggcgc ctcgccgcga
     5281 cgacggtgct ggtcaccggg gcggccgagc tgacgggacc gctggcggag gttctcggag
     5341 cgaccggtta cgggtcggtg cggccgctgg ccgaagcggc gctcctggtg aacccggcgg
     5401 aggtcctccg cacggccggc gatggtccgg cgcggtccgc ggaccccgcg gacccggcgg
     5461 agatcctcgg ctcggccgcc tacaacgcgg ttccaccgct cgtcgaagcc gcgttccccc
     5521 tggccccggc cgacaccgtc ctcgtcgccg cctacaccca ccccgcctac cgggagctca
     5581 gcgcactcga cgcgttctgc accgagcacg gcgtgcggtt cttccccttc cgctgcgaac
     5641 gcggccaggg catcgccggt cccgcggtcg aaccgggctt cggccccgac ttcgccgacg
     5701 tgctcgcgcg gcgccgctcg gccgccctcg acgcccgggt ccccgacgcc ttcgccgccg
     5761 ccgaggcccc gcccgggccg cggttccggc gaaccgacgc ccgctggctg gtcagcgccc
     5821 tcgccgtcca gctcgaacgg tggatcgccg gcgagcccgc cgagacgacg accggcgagc
     5881 tcgagctcga ccccgtccgg ctgaccgcgc tcccccggcc cgttctcccc gtgcccgacc
     5941 ggccccggcc ggtccgggac cagggcctgc ggccggacct gctcgtcgac gaccggaccg
     6001 ggatcgtgac cgcggtgcac gaactgcccg ccgcgccggg gatgcccgcc cggctcaagg
     6061 tgtgcgcggt ggacgtcgcg gacatgcggc gggtcgtcga ctggcccaac gaccgccagg
     6121 cgttcgggac gtcgtggcac gacttcgagc cggcccgcga gtccgcgatc ggcgaggccg
     6181 tcgaacgcta ctgcggcagc tggctgcccc cggaacgcga actccgccac ggcagctacg
     6241 accagctgcg ccgcgccggc gtcccggcgc tggacccgcg ccggctcaac ctctactcgg
     6301 cccggcagca ccgcaccccc ggcttcccgt tcgcgccgct gaccaccgac gcggaatgcg
     6361 cctgggtgga agccttttcg cacacgacga aggagcccgt atgggtaccc gcgtgcctcg
     6421 tctcgcaggc acccgaaccc ggtggggcgc gcttcaccga cccgctgatc gccgggctgg
     6481 ccggtggcac gagcgccgag cacgccgtca cctccggcct cgaggaggtt ctcgaacggg
     6541 acaccacgat ggtgtggtgg gccaacgcac cgcggctgcg cgccctgccg gtgccgcccg
     6601 aggtgcgcgc gctcgtcgcc gacaccaccg aaacctacga agtcacgctg atcccgctgg
     6661 acaacgagtt cgccgtcccg gtcgtcgcgg cggcggtgtt cgaccgggcg cgccgctggc
     6721 tgtcgatcgg gttcgccacg cgccccgacg ccctggaagc ggccaagaag gcgctcgccg
     6781 aagggttcac gctgcagcac acctgcctcg cgctcgacga cgagcgcgag ctggccgcga
     6841 tgcgcgacga cctgccgcac ctgggcaacc tgaagccgta ccgggccgac cggcgctacc
     6901 tcgactccta ccgcgcggac ttcggcgacg tcgtggacct gctgtgccag caacaggtct
     6961 acctcgaccc gcgggcggcg gcccacgtcg caccgtgggt ccgcgacctg ccggagcgga
     7021 gctgggaagg cctgcccgcg ctcggcgaac ggacgctcaa gacctaccag gaccgcgtcg
     7081 aggcacgcgg gttcgaggtg ctcagcgtgg acctcaccac ccgtgacgtc gccgcggccg
     7141 ggttccacgc cgcgcacacg ctggtgccgg ggctggtggc caacttcccg gccgggctgc
     7201 cgtactgggg cgacggccgc gtgcgccgcg cggcggtcga cctcggctgg cgcgcgcacc
     7261 ccctgcccga agcgcggctg aacaccttcc cgctccccca cgcctgagga cgcaccgatg
     7321 gccgtcaccg cactgctcgg gctgccccgc acccggggcc gcctcccgct ggtcgccgcc
     7381 cagggcgtgg acgcgctcgg caccggcctg ttcctgccgt tcgccgtcgt ctacttccac
     7441 gccgccaagg gacttccgct gacggccgtc ggcgcggcgc tctcgctcgc cgcgttgctc
     7501 gccctgccgg ccgggctctg cgcgggcccg ctggtggacc gtttcggccc gcggcgggtg
     7561 gtcgtcgcgg ccaacctgct gcgggtgctc accttcaccg ggtacgtctt cgccgggtcg
     7621 ccgccggtcc tggtggtgct ggcggcgctg acgttctggg gcgaaggcct gttctggccc
     7681 gcgtccggtg cgctggtggc gcaggtggcc gacgacgggc agcgcgcccg ctggtacgcg
     7741 atggagcgca cgctgcgcaa cgtcgggatc ggcctcggcg ggcttgccgg cagcgtgctc
     7801 gtcgtggtgg gcggctacgc ggcgatcgtc gtgctcaacg ccgtgagctt cctcgtcgcc
     7861 gccgtgctcg tggcgacctg gcggggccgg gcgggcgaag ccccggcgcg gaccaccgcg
     7921 ccgcgcgggg gttaccgggc cgtgctcgcc gaccgcgcgt tccgccgcgt gctggtgacc
     7981 gtcttcgtct tcgcgttgtg cgacctggct ttgacggtgc tgctgagcgc gtacgtgctc
     8041 gacgcgctgc ggttgccggc gtggcagccc ggtgtgctgt tcgccctcaa cacggtgctg
     8101 gtggtgctcg cgcagacggt cgtggccggg cgggtggagc ggttccgccg ggggcggacg
     8161 ctgcaggtcg cggccgtggt gtgggccggg gcgttcctgc tctacgcggc cgtgccgctg
     8221 gtgacggcgc tgcccgcgtt cgccgtgctg acgctggcca ccgcggtttt cacggtggcc
     8281 gagctgctgc aggcgccgac cagcagcgcg ttgaccgtgg cgctcgcgcc cgcccacctg
     8341 cgcgggcgct acctcgggct cgaggaagtg ctctggggtg cggcgcgggt gctggccccg
     8401 gtgaccttca cggcgttgct ggcgggtggc ccgcagctgc cgtggctcgt gctcgccggg
     8461 tgctgcctgc tggcggtggc cgtcctgacc gggctgaagg cggtcccgga gccggtgcgg
     8521 gagacggcga atgcctgact ggaccgcggt caccggcctc taccaccagg tctaccgcga
     8581 acctccctat ggggaaagcg aagcggacgt ggcccggttc cgcgaaacgc tggcgcagca
     8641 cgcggagttg cccgggttcg cgctgacggc cgtccacagt ggagacgtgt tggacgggtt
     8701 cgcctacggc gtcggccgcg aggcgggctg gtggccgtcc ccggcggcgg ccgccccgcc
     8761 gccgtggctc gccgggcgtc cgctgttcta cgtgtacgag ctcgccgtcc ggccggggcg
     8821 gcgcgggcgg ggccacggcc gggacctgct cgaccggctg ctgcgcgacc gggccgagcc
     8881 gtgcgcgctc ctcgccgcgg cgacggcggc cccggcgcac gcgctgtacc ggcggtgggg
     8941 ctgggaggcg gtcggcgtgt tcaggccggg tgtctcggaa ctgctggctc ggcggctctg
     9001 agagcgtcct cggtcaccac gaaaagcccg cccaccaccg aaaacgccag cacggtcatc
     9061 acgacgaaca ccacgtgcag cccgaaggcc tgcgcgagcg cgccgccggc cagcgcgccg
     9121 agcgacttcg tgccccacgc gaccagccgg tggctgctgg tgaggcggcc gagcagccgg
     9181 tccggcgtga tccgctggcg cagcgacacc atgaccacgt tcgacaccag gatgcccgcg
     9241 ccgccggcga agaaggccgc accgacgacg tacgggtcgg cggtcacggc gagcacgccg
     9301 accgtcaggc cgccggcgag ccacgacacc cggagcgcgc ggacgcggcc gagccggcgt
     9361 tccaccgtct cggcgagcag cgacccggcc aggccgcccg cggcggtcgt cgccgtcagc
     9421 aggccgaagg ccggtccgga caggcccatc gccgagccgg ggccgaccgc gtagaggacc
     9481 aggaccgcga acgtggctcc ggtggcgaag ttgaacgtgc cgaccatgag ggagaaggtc
     9541 cgcagaacct tgtggcgggc gaggaaccgc aggccctcgg cgatgtccgc gcgcagggtg
     9601 gcgggtcggt cgccgcgcgg gatccggtac gagccccgca cgaacagcag cgcggcgacc
     9661 gccaccgccc acagcaccac cggcgtgacg aacgcggcca gcaccccggc ggtgaccagg
     9721 accccggcca gcggcggccc ggcgaactcg ttggcggtct gttcgaccgc gtagacccgg
     9781 ccgttggcgc gggcgaggtc ggcccgcccg acgacctgcg ggacgatcga ctgcgcggcg
     9841 gtgtcgtaga gcgtctcggc ggtgccggca cccagcgcga cgacgtagag cgcccagatc
     9901 gagcccccgc cgtaagcggt cagcaccgcg acgacggcga cgagcgcccc gcgcaggacg
     9961 ttggcgccga gcatcgcgcg gcggcggtcg agccggtcga cgagcgcgcc ggcgggcagc
    10021 gcgaagagca gccacgggac gctgaaggcg aacgcgagcc ccgcgaccag cgccggctcg
    10081 cgcgtgtacc cgacggcgac cagcggcagg gcgaccttga ggacgccgtc ggcgaggctc
    10141 gacaggcccg cggccgtcca cagccgccag aaggtccggt gcatgaatgg aggatcgcac
    10201 atcgatggac ttttctccat cgatccgagc tttccccatc gatcgactac gctgcgtcca
    10261 tgaacgaacc acgccgccgc cgtccggcga cgccggagga agccaaggcc ctcggccacc
    10321 cgctccggtt gcgcatcatg cggctgtgcc tggtcgacga gctgacgaac aagcaactgg
    10381 ccgaccgcct cgaccgtgac ccgggcacgg tcctgcacca cgtccgccag ctggtttcag
    10441 ccggcctgct gcacccggcg ccggtccgga cggggccgag cggagcgctg gagaagccct
    10501 accgcgcgga caacgagacc tggtggctcg acggtcccct ggccaccgcc gacgcggaga
    10561 cgcggttcgc gccgatcgag atcttccagg aggagctgca cgcggcgggc ccggattcgg
    10621 tggcggtgca cgagaagttc ctgctgcacc tgtcaccgga cgaggtggaa gagctggacc
    10681 gccggatcct ggcggtgctg gacgagtacg tggcgaccga ccacgagcgc ccggaccggc
    10741 cggcgatcgg cgggatcttc gtgctgcacc acctgaagcc cggaacctga accgccccct
    10801 gcccggggga ggtgggcagg gggcggtccg gttggtcagg tcagtggttc accttgaacc
    10861 acggctgcga ccagccgagg taggtctggc cccacttgct cttgatctgg gcgatcgtcg
    10921 tctcggtgta gctgccgtag ccgttgatgt cgttcgacag gaacttgccg ccgccgatgg
    10981 agatcatcgt gtggcccgcg ccgctgctct tgaagaacgc gaggccgccc gccgggacct
    11041 gggtgtcgcc cgggtgcttg aacgaggccg ggatctgggt ccagtggatg taggccgtct
    11101 ccgagcccga cgcggtccag ccgtagttct gcgcgttgat ccggtcgcac cagccctcgt
    11161 aggagtcgag gtcgttcgtg tgcacgcggg tcttggcgta ggccaccgcc tgcgcgcagg
    11221 tcttcgggtt cccggtgccc gcggtggtgc aggacagcgt cgagcccgcg cagtccgggg
    11281 ccacccggcc gtccgagccg gtgtagacgt aggcgtcgct gatgtagccg tcgccgacct
    11341 tgtcccagat gtccgacgtg ccatacttgc cgtcgacggt cgatccgttg gtctggcagg
    11401 agatggtgac ggcggtgccg tccgcgatcg tgccgaccgc gctgccgccg gtgctcggcg
    11461 tggaccgcac ggtcagcgcg atccccgagt ccgtgtgcac cgtgcctgtc gccgccaggg
    11521 ccggggtggc gccggccagt gcgatcgccg cggcgatgcc ggccacgccg gccacccgct
    11581 tcccgatccg aattctcatt tgtccgtccc tccgggattc gttgtgggga cagcgtggcg
    11641 gccgccggta caagggccgt acaaacgcac gcaccggcct ctggagatgc ggcaaacagg
    11701 acgaagcccc gccggagggg tgcccggcgg ggcttcgacg cgaactcagg ccaggtactc
    11761 ggccgccgcc cgcaggctca cgtaggagcc gctgaacgtg tcgatccgcg ggccgccgtc
    11821 gccggccggt cccggtgccc cggtctcgac gcggatgtag tccacagtgg acaacgcggc
    11881 cagcagctcc cgcacgcgcg ggtgccggtg tgcctcccgc gtgaccacga caacgctgcg
    11941 gaacccccgc gccgcttcga gaaccgcgtc ggcgtcgtcc ggggtcgcgg ccagtacccg
    12001 cgttcccggc accagctcgg ccaggtgcgc accgagtccc cacggcatcg gaccggccgc
    12061 gatggtcggc tcggtctgga cgtcgaccac cagcggcggt ccggccagcc gcacgtcgcc
    12121 ccgcacgcgc aacgccttgc gcgccgcttc cagccccagc cgccggtcga ccgggccgac
    12181 cgtcgccggc gcggggtcgg tgccgagcgc cgccgtccgc gaagccgctt cggaaagccg
    12241 ctccaggggc agctcccccg ccgccaccgc ggccacgatc gccgcgacgc aggcgtccag
    12301 gtgctcg
//
