LOCUS       NZ_CP095376            10837 bp    DNA     circular CON 06-NOV-2024
DEFINITION  Amycolatopsis sp. FBCC-B4732 chromosome, complete genome.
ACCESSION   NZ_CP095376
VERSION     NZ_CP095376.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis sp. FBCC-B4732
  ORGANISM  Amycolatopsis sp. FBCC-B4732
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 10190959)
  AUTHORS   Choi,A.
  TITLE     Complete Genome Sequence of Amycolatopsis pretoriensis strain
            FBCC-B4732
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10190959)
  AUTHORS   Choi,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2022) Bioresources Collection & Research Team,
            Nakdonggang National Institute of Biological Resources, Donam 2-gil,
            Sangju-si, Gyeongsangbuk-do 37242, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 75.1x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023008405.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 10:39:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,364
            CDSs (total)                      :: 9,298
            Genes (coding)                    :: 9,177
            CDSs (with protein)               :: 9,177
            Genes (RNA)                       :: 66
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 50 of 121
            Pseudo Genes (incomplete)         :: 81 of 121
            Pseudo Genes (internal stop)      :: 14 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:14:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2733272
            Orig. end                         :: 2744109
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095376.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from NNIBR.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10837
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="16"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10837
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10837
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5837](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5837
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    104..119
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.48"
                     /tool="antismash"
     misc_feature    complement(305..319)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.76"
                     /tool="antismash"
     gene            749..1744
                     /gene="mgrA"
                     /locus_tag="MUY14_RS11650"
                     /old_locus_tag="MUY14_11650"
     CDS             749..1744
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="mgrA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227010.1"
                     /locus_tag="MUY14_RS11650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_11650"
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="WP_247022987.1"
                     /transl_table=11
                     /translation="MSPFAAAGNRYADMPYRRAGRSGLKLPAVSLGLWHNFGDDKPLDV
                     QRAVLRRAFDLGVTHFDLANNYGPPPGAAEANFGRHYAADFRPYRDEILVSSKAGYLMW
                     DGPYGEWGSRKNLLASLDQSLARTGLDHFDVFYSHRPDPETPIEETMGALDTAVRSGKA
                     LYAGISNYSPEQTEAALAALRDLGTPLLIHQPSYSILNRWVEDGLLDTLDEHGTGSIAY
                     SPLSQGLLTDRYLDGVPADSRAAGASPFLTRDRLTEETLDKIRALNDVAKRRGQTLAQL
                     AIAWVLRRGRVTSALIGASSVAQLENTVAATANLEFTDDELAEIDRIAAR"
     gene            complement(1746..2894)
                     /locus_tag="MUY14_RS11655"
                     /old_locus_tag="MUY14_11655"
     CDS             complement(1746..2894)
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_function="GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015802316.1"
                     /locus_tag="MUY14_RS11655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_11655"
                     /product="cellulose-binding domain-containing protein"
                     /protein_id="WP_247022988.1"
                     /transl_table=11
                     /translation="MPSRFAGALAALCLAAAGTTAVLAAGPAAAAPACSVAYRVNQWQT
                     GYTADLTVTNGATALSSWALTWHYPGGQTVTSAWNATVRQSGTAVTAESMPYNAALPAG
                     GAVSFGLQGTGSGADPTDFALNGVACGDPAPPITTTATTPITLPTTTTSAPPPAGCADA
                     ALCDDFEQQTGGTPGGRWTVGAANCQGTGTVTVDASVAHSGSRSVKVTGQGGYCNHAFL
                     GTSVSGTGVLYGRFWVRHTTALPAGHVTFLAMRDSTDGKDLRAGGQNRALQWNRESDDA
                     TLPAQSPAGVAQSVPLPTGAWSCFEFRLDGAGGQLRTWLGSAEVPGLVVDGVPTADVDQ
                     QWLARSWHPAVTDLRLGWESYAGDADTLWFDDVAVGASRIGC"
     misc_feature    complement(2984..2998)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: medium, score: 26.66"
                     /tool="antismash"
     misc_feature    2985..2999
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: strong, score: 27.52"
                     /tool="antismash"
     misc_feature    complement(3273..3287)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: strong, score: 27.15"
                     /tool="antismash"
     misc_feature    3274..3288
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: strong, score: 28.62"
                     /tool="antismash"
     misc_feature    complement(3318..3332)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: strong, score: 20.64"
                     /tool="antismash"
     misc_feature    complement(3450..3459)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.29"
                     /tool="antismash"
     gene            3556..5004
                     /locus_tag="MUY14_RS11660"
                     /old_locus_tag="MUY14_11660"
     CDS             3556..5004
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868663.1"
                     /locus_tag="MUY14_RS11660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_11660"
                     /product="hypothetical protein"
                     /protein_id="WP_247022989.1"
                     /transl_table=11
                     /translation="MSRQVKPGSKPYRAQLRDRLAALGFPETAIPARVAENLVVECGIP
                     PRTAWRLASELSLDVAAHRYNAATGDPRAGMRGTRIWEYEQWPERGVRPTIAALRVLAG
                     VYGTSWKSLLGLRDLEQLPAKDLAEYHAEEEPVAVVPVSAPPARAEVRGEARPDEEIGA
                     SALDDAILLTQTNVDDVQLDDLWSDLDFLGGAYTRTAPDAVLGQLAVIDERTSALLKGR
                     QRPKQTQDLLLVGAKCSAMMAWIAGDLGRYRLSRQLNSAAWLYTQYADDFLARRWVRTS
                     QARVAFWSGNGIESARLAADGLSYHVGPRLTDAPLILAEARGWSSVQAEPQVLDAIARW
                     TSIEDPDLGAGGEDRFFNITKDRRHYMAGTSLLSVGQASAALREFTTAREAYEKLSLEH
                     RWEAMDPMIRIDTGRAHLRLGDLDGAAAQVEQVLAADVGSQPDMVHSMLKVLATELTEP
                     RWRAAVTARNLAEAVLDAHASARP"
     gene            5001..5837
                     /locus_tag="MUY14_RS11665"
                     /old_locus_tag="MUY14_11665"
     CDS             5001..5837
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227007.1"
                     /locus_tag="MUY14_RS11665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_11665"
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_247022990.1"
                     /sec_met_domain="AfsA (E-value: 1.3e-14, bitscore: 53.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGDPTRAEVAPDAPARPGTGHEDDWALEGHTRRDVVVVADRFAR
                     FARGERVLTVSGLLALLAADDTDPEPASCHWVVHLGQGIDATDHDLLEAAREKSGVTVT
                     FADPRAFRRVPERSVLVHKDRSENVLLAGVHSPSAGHCQAELRIHRDNELILDHHTGEH
                     VQGIVIVEAMRQICIAQFETAIRPGLATPAYAGVWKRIDLSFQDFLFPLPATVESLIEE
                     SDLGRESNLKFRATTAVRQQGRTVATANIEYSMIAQGRIDALERRKADQATRAYLG"
     gene            5873..6676
                     /locus_tag="MUY14_RS11670"
                     /old_locus_tag="MUY14_11670"
     CDS             5873..6676
                     /GO_function="GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638539.1"
                     /locus_tag="MUY14_RS11670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_11670"
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="WP_247022991.1"
                     /transl_table=11
                     /translation="MSEEPDRSASFAQGLKLIQQLGGADRPAVLDLFESIGEAEFGEQC
                     VGFIYGDVYHRPGLELPERQLTTVAALTALGYAGSQLQFHAKAALNVGCTRRQLVEAVI
                     HVSSFAGFPATLNALTALKAAFEGLPEDEPAAEPAEVPWAGIEDRYERGLAAMKAVDGE
                     AGEKVAVALADIAPDLAGYIIEFTFGELYTRPGLSLRHREIVTIAACVALGTALPQLKV
                     HIHGLLNVGGTEKEVVETVLHLAFYCGFPAALNAIGAVREVFAQR"
     gene            6673..7374
                     /locus_tag="MUY14_RS11675"
                     /old_locus_tag="MUY14_11675"
     CDS             6673..7374
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227005.1"
                     /locus_tag="MUY14_RS11675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_11675"
                     /product="HAD family hydrolase"
                     /protein_id="WP_247022992.1"
                     /transl_table=11
                     /translation="MSGFGKLRAVALDCDGVLIDDTYLAMIARFVTAHGGVYDAAAERD
                     VIGLRDIVVAEKITALCGLDQPPEETLKQLWAARQEYLAEHPIRVAEGARDCLEALSKL
                     PVRLVCYGGRTREHTFDRYLGEFVDLLDPAVPYVSINEHRPGVEHITRTVLGAGFDEIV
                     FVDDVSRVAEDARAHGAGFVGFPSSPAHARQRDFMAGHGVRHFAASLAELTPELLEIVD
                     AELATSTHWLR"
     gene            7401..8402
                     /locus_tag="MUY14_RS11680"
                     /old_locus_tag="MUY14_11680"
     CDS             7401..8402
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_function="GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (18-330). E-value: 1.6e-25.
                     Score: 81.8. Matches aSDomain:
                     nrpspksdomains_MUY14_RS11680_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003084464.1"
                     /locus_tag="MUY14_RS11680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_11680"
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="WP_247022993.1"
                     /sec_met_domain="ADH_N (E-value: 1.8e-08, bitscore: 33.3,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 2.5e-22, bitscore:
                     78.4, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYFSLPRPGAGLVSGERDVPEPGPGQVLVRLRGWAVNARDLMIV
                     KGFYPKPVKPDVVPLSDGAGEVVAVGEGVRAWATGDRVAATYFPDWLSGPGTPEKTADD
                     LAGTLDGVLAEYAVFAEDALVGVPAHLDYAEAATLPSAGVTAWRAVVEEGGLVPGQTVL
                     TLGSGGLSTFALQFAALGGAHVVSTSGSDEKLERLRELGAAETLNHATTPEWGAAVAQL
                     TGGGVDHVVDVGGGGTIGQSMLAARYGGHVSVAGVLTHEGGADPILVLVKQLTLRGLTN
                     ASRETFQAMNRAIEHTGLRPVIDRRFAFDEVEEALKHLESGSHVGKVVLESA"
     aSDomain        7455..8390
                     /ASF="ER configuration inconclusive"
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MUY14_RS11680_PKS_ER.1"
                     /evalue="1.60E-25"
                     /label="MUY14_RS11680_PKS_ER.1"
                     /locus_tag="MUY14_RS11680"
                     /protein_end="330"
                     /protein_start="18"
                     /score="81.8"
                     /tool="antismash"
                     /translation="DVPEPGPGQVLVRLRGWAVNARDLMIVKGFYPKPVKPDVVPLSDG
                     AGEVVAVGEGVRAWATGDRVAATYFPDWLSGPGTPEKTADDLAGTLDGVLAEYAVFAED
                     ALVGVPAHLDYAEAATLPSAGVTAWRAVVEEGGLVPGQTVLTLGSGGLSTFALQFAALG
                     GAHVVSTSGSDEKLERLRELGAAETLNHATTPEWGAAVAQLTGGGVDHVVDVGGGGTIG
                     QSMLAARYGGHVSVAGVLTHEGGADPILVLVKQLTLRGLTNASRETFQAMNRAIEHTGL
                     RPVIDRRFAFDEVEEALKHLESGSHVGKVVL"
     CDS_motif       8343..8390
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MUY14_RS11680_0001"
                     /evalue="1.00E-04"
                     /label="PKSI-ER_m9"
                     /locus_tag="MUY14_RS11680"
                     /protein_end="330"
                     /protein_start="314"
                     /score="15.3"
                     /tool="antismash"
                     /translation="ALKHLESGSHVGKVVL"
     misc_feature    complement(9214..9227)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 21.45"
                     /tool="antismash"
     misc_feature    10123..10136
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: weak, score: 17.99"
                     /tool="antismash"
     misc_feature    10317..10333
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.44"
                     /tool="antismash"
ORIGIN
        1 gactaccgtg gcgccaacgc gaacatgcac accgtcgagg cgttcctggc cgccgccgac
       61 gtcaccggcg accgggtctg ggccacccgc gcgctgtcca ttgtggacca cctggtgcac
      121 ggcgaagccc gcgcgcacgg ctggctgctg ccggagcact acgacccggc ctggcgggtg
      181 cgcctcgagt tcaaccggga cgagcccgcg cacccgttcc gcccgttcgg cgcgacgatc
      241 gggcacctct tcgagtgggc gcgcctggcc gtgcacctca agcgcgcgct cggcggcgac
      301 gccccggacc ggctggtccc ggacgcggcg gcgctgttcg agacggcgac ccggaccggc
      361 tgggccgtcg acgggcaccc cggcttcgtc tacaccacgg acttcgacgg cacgccggtc
      421 gtgcgcaccc ggctgcactg ggtcgtggcg gaggcgatcg ccgccgcgtg gacgctgcac
      481 caggaaaccg gcgagccggt ctacctccag cggttcgagg agtggtgcgc gcacgccgag
      541 gcctgcttcg ccgaccggga acgcgggtcg tggcaccacg aactcgaccc ggagaaccgc
      601 ccggccgcga cggtctgggc gggcaagccg gacgtctacc acgcctatca ggcgacgctg
      661 ctgcccacgc tcccgcccgc cgggtcgttc gccggcgcgc tcaccgcgcg tcgctagact
      721 ccccacgacc cgccgaccga ggagaaccat gtcgcctttc gccgccgccg ggaaccggta
      781 cgcggacatg ccgtaccggc gggccggacg cagcgggctg aagctgcccg ccgtgtcgct
      841 cggcctctgg cacaacttcg gggacgacaa gcccctcgac gtccagcgcg cggtgctgcg
      901 ccgggcgttc gacctcggcg tgacgcactt cgacctggcc aacaactacg ggccgccgcc
      961 cggcgcggcc gaggcgaact tcgggcggca ctacgcggcc gactttcgcc cgtaccgcga
     1021 cgagatcctg gtgtcgtcca aggcgggcta cctgatgtgg gacggcccgt acggcgaatg
     1081 gggctcgcgc aagaacctcc tcgcgagcct ggaccagagc ctcgcccgca ccggactgga
     1141 ccacttcgac gtcttctact cccaccgccc ggacccggag acgccgatcg aggagaccat
     1201 gggtgccctc gacaccgccg tccggtcggg caaggcgctg tacgcgggca tctcgaacta
     1261 ctcgcccgag cagaccgagg ccgcgctcgc cgcgcttcgc gacctcggca cgccgctgct
     1321 catccaccag ccgtcgtact cgatcctcaa ccggtgggtc gaggacggtc tcctcgacac
     1381 cctcgacgag cacggcaccg gctcgatcgc gtactcgccg ctgagccagg gcctgctgac
     1441 cgaccgctac ctcgacggcg tcccggccga ctcgcgggcg gccggcgcga gcccgttcct
     1501 cacccgcgac cgcctcaccg aggagacgct ggacaagatc cgcgcgctga acgacgtcgc
     1561 gaagcggcgc gggcagacgc tggcccagct cgccatcgcc tgggtgctgc gccgcgggcg
     1621 cgtgacgtcg gcgctgatcg gcgccagcag cgtcgcgcag ctggagaaca ccgtcgcggc
     1681 caccgcgaac ctcgagttca ccgacgacga gctggccgag atcgaccgga tcgccgcccg
     1741 gtagctcagc agccgatccg cgaggcgccc acggcgacgt cgtcgaacca cagcgtgtcg
     1801 gcgtcgccgg cgtagctctc ccagccgagc cgcaggtcgg tcacggccgg gtgccaggac
     1861 cgcgcgagcc actgctggtc gacgtcggcg gtcgggacgc cgtcgacgac gagcccgggc
     1921 acttcggccg aacccagcca cgtccgcagc tgcccgcccg cgccgtcgag ccggaactcg
     1981 aagcaggacc aggcgccggt gggcagcggg acgctctggg cgaccccggc cgggctctgc
     2041 gcgggcaggg tggcgtcgtc ggactcgcgg ttccactgca gcgcgcggtt ctgcccgccc
     2101 gctcgcaggt ccttgccgtc ggtgctgtcg cgcatcgcga ggaaggtgac gtgcccggcg
     2161 ggcagcgccg tcgtgtgccg gacccagaac cgcccgtaga gcacgccggt gccgctcaca
     2221 ctggtgccga ggaacgcgtg gttgcagtag ccgccctggc cggtgacctt caccgagcgg
     2281 ctgcccgagt gcgccaccga ggcgtcgacg gtcaccgtgc cggtgccctg gcagttcgcc
     2341 gcgccgacgg tccagcggcc gcccggggtg ccgccggtct gctgctcgaa gtcgtcgcag
     2401 agcgcggcgt ccgcgcagcc cgcgggcggg ggcgccgacg tcgtcgtcgt gggcaaggtg
     2461 atgggcgtcg tggcggtcgt cgtgatcggg ggagcggggt cgccgcacgc gacgccgttg
     2521 agcgcgaagt ccgtgggatc cgcgccgctc ccggtgccct gcaggccgaa cgacaccgca
     2581 ccgccggcgg gcagggcggc gttgtagggc atgctttccg cggtgacggc ggtcccggac
     2641 tggcggacgg tcgcgttcca cgccgaggtg acggtctggc caccggggta gtgccatgtg
     2701 agggcccacg aggagagcgc ggtcgcgccg ttggtcacgg tgaggtcggc ggtgtagccg
     2761 gtctgccact ggttcacccg gtacgcgacc gagcaggcgg gcgcggcggc ggccgggccg
     2821 gcggcgagga ccgcggtggt gcccgccgcg gcgaggcaga gggcggcgag ggcaccggcg
     2881 aacctgctgg gcatcgggat cctcccggga cgcggacagg ggcgccgtgg cctgcaccac
     2941 gacgcccctg ggtggtggcc gcgttctggc gaggcgacct ggcatgggag cgcttccagc
     3001 cgccgaaaaa gtaacccccg gatgtcgctc ttgtaaagcg ggacccggac gcgccgcgat
     3061 ccggtggtcg gttttccggc gccgccgggc ccgtgctcgg ccactgaacg gtactgaacg
     3121 gccgaacccg ttgtcgggca acgggtgtgc tgatcgcgct gcgctaccgc cccgcgctcc
     3181 gcgatgccgc cgttggggtg gaatccggca atccggttca agatcacgac gggtcctcgc
     3241 cggagccgga acggactcgg tgtcccgctt gggtgggagc gctctcattt tctccgggtg
     3301 gacggtcgag tcctctgtgg aacggatcgg cccgcgcggg cgtcgaagcg ccgtgcccga
     3361 aaccgcagac cgacgtccgg ccgcacctcc tccccgccgg gcccgcccgg tccgcccggt
     3421 gcggaaaccc ttgttgcggg ctcaacatct cgaaaatcac acggtcccgg cagcggtgac
     3481 gccctgggcc cccgctgtca cgctggggta ctgtcatccg tgtgcaccag tactccgaac
     3541 cgcactgggt gaacgatgag ccgacaggtc aagccaggga gtaagccgta ccgtgcacaa
     3601 ctgcgcgacc ggctggccgc tctgggcttc cccgaaacgg cgatcccggc ccgggtggcg
     3661 gagaacctgg tggtggaatg cgggatcccg ccgcgcaccg cgtggcggct ggcgtccgag
     3721 ctttcgctcg acgtggcggc gcaccgctac aacgccgcga ccggcgatcc ccgcgcgggc
     3781 atgcgcggga cgcggatctg ggagtacgag cagtggcccg aacgcggtgt ccggccgacg
     3841 atcgcggcac tgcgggtgct ggccggcgtc tacggcacca gctggaaatc cctgctgggc
     3901 ctgcgtgatc tcgagcagct gccggccaag gacctcgccg agtaccacgc ggaagaagaa
     3961 ccggtggcgg tcgtgcccgt ctccgcgccg cccgctcgcg cggaggtgcg cggcgaagcc
     4021 cggccggacg aggaaatcgg ggcaagcgca cttgatgacg cgattctgct gacccagacc
     4081 aacgtcgacg acgtccagct cgacgacctg tggtccgacc tggacttcct gggcggcgcc
     4141 tacacccgca ccgcgcccga cgcggtcctc ggccagctcg ccgtcatcga cgaacggacg
     4201 tcggcgctgc tgaagggcag gcagcggccg aagcagacgc aggacctgtt gctcgtcggg
     4261 gcgaagtgct cggcgatgat ggcgtggatc gcgggcgacc tcggccggta ccggctttcg
     4321 cgccagctga actccgccgc ttggctctac acgcagtacg ccgacgactt cctggcccgc
     4381 cgctgggtgc gcacttcgca ggcgcgggtg gcgttctggt ccggcaacgg catcgagtcc
     4441 gcgcggctgg ccgccgacgg gctcagctac cacgtcggcc cgcgtctcac ggacgcgccg
     4501 ctgatcctcg ccgaggcgcg cggctggtcg tcggtgcagg ccgagccgca ggtgctggac
     4561 gcgatcgcgc gctggaccag catcgaagac ccggacctcg gcgccggtgg cgaagaccgg
     4621 ttcttcaaca tcaccaagga ccggcgccac tacatggccg ggacctcgct gctttcggtc
     4681 ggccaggcgt cggccgcgct gcgcgagttc acgaccgccc gcgaggccta cgagaaactg
     4741 tccttggaac accgctggga ggccatggac ccgatgatcc ggatcgacac gggccgcgcg
     4801 cacctgaggc tcggcgacct cgacggcgcc gccgcgcagg tggagcaggt gctggccgcg
     4861 gacgtcggca gccagccgga catggtgcac tcgatgctga aggtgctcgc caccgagctg
     4921 accgagccgc gctggcgcgc ggcggtgacg gcgcggaacc tggccgaggc ggtcctggac
     4981 gcccacgctt cggcgcggcc gtgaccggcg accccacccg ggcggaggtg gcgccggacg
     5041 ctcccgcgcg ccctgggacc gggcacgagg acgactgggc gctcgaaggc cacacccgcc
     5101 gggacgtggt cgtcgtcgcc gatcgcttcg cgcggttcgc ccgcggggag cgggtcctca
     5161 ccgtgtccgg cctgctggcc ctgctcgccg ccgacgacac cgaccccgaa cccgcctcct
     5221 gccactgggt cgtccacctc ggccagggca tcgacgccac cgaccacgac ctgctcgaag
     5281 cggcccgcga gaagagcggg gtcaccgtga ccttcgcgga cccgcgggcc ttccggcgcg
     5341 tccccgaacg ctcggtcctc gtgcacaagg accgctcgga gaacgtcctg ctggcgggcg
     5401 tgcacagccc gtcggccggg cactgccagg ccgagctgcg gatccaccgg gacaacgagc
     5461 tgatcctcga ccaccacacc ggcgagcacg tgcagggcat cgtgatcgtc gaggcgatgc
     5521 ggcagatctg catcgcccag ttcgagaccg cgatccggcc aggcctggcc acgcccgcgt
     5581 acgcgggggt ctggaagcgg atcgacctgt cgttccagga cttcctgttc ccgctgccgg
     5641 ccacggtcga gtcgctcatc gaggagtccg acctcggccg ggagagcaac ctgaagttcc
     5701 gcgccaccac ggcggtgcgc cagcaggggc gcacggtggc caccgcgaac atcgagtact
     5761 cgatgatcgc gcaggggcgc atcgacgcac tggaacgccg caaggccgac caggcgaccc
     5821 gcgcctacct cggctgagcc gaaacccact ccggaaggga aaccacacca cgatgtccga
     5881 agagccggac cgttcggcgt ccttcgcgca gggactcaaa ctcatccagc agctcggcgg
     5941 ggccgaccgc ccggccgtgc tggacctgtt cgagagcatc ggcgaggccg agttcggcga
     6001 gcagtgcgtc gggttcatct acggcgacgt ctaccaccgg cccgggctcg agctcccgga
     6061 gcggcagctg acgaccgtcg ccgcgctcac cgcgctcggc tacgcgggct cgcagctgca
     6121 gttccacgcc aaggccgcgc tcaacgtcgg ctgcacccgc cgccagctgg tggaggcggt
     6181 catccacgtc agctcgttcg ccgggttccc cgccacgctc aacgcgctca cggcgctcaa
     6241 ggcggcgttc gagggcctgc ccgaggacga gccggcggcc gagcccgccg aagtgccgtg
     6301 ggcgggcatc gaagaccgct acgagcgcgg gctggccgcg atgaaggcgg tcgacggcga
     6361 ggcgggggag aaggtcgccg tcgcactggc cgacatcgcg cccgacctgg ccggctacat
     6421 catcgagttc accttcggcg agctctacac ccggccgggg ctgagcctgc gccaccgcga
     6481 gatcgtcacg atcgccgcgt gcgtcgcgct cggcacggcg ctgccgcagc tgaaggtgca
     6541 catccacggg ctgctcaacg tcggcggcac cgagaaggag gtcgtcgaga ccgtcctgca
     6601 cctggcgttc tactgcgggt tcccggccgc gctcaacgcg atcggagcgg tgcgggaagt
     6661 gttcgcgcag cgatgagcgg gttcgggaag ctgcgcgccg tcgcgctcga ctgcgacggc
     6721 gtgctcatcg acgacaccta cctggccatg atcgcccggt tcgtcaccgc gcacggcggg
     6781 gtctacgacg ccgcggccga gcgggacgtg atcgggctgc gcgacatcgt cgtcgccgag
     6841 aagatcaccg cgctctgcgg cctcgaccag ccgcccgaag agacgctcaa gcagctgtgg
     6901 gccgcgcggc aggagtacct cgccgagcac ccgatccgcg tcgccgaagg ggcgcgggac
     6961 tgcctggaag cgctctcgaa actgcccgtg cggctggtct gctacggcgg ccggacccgc
     7021 gagcacacct tcgaccggta cctcggcgag ttcgtggacc tgctcgaccc ggcggtgccg
     7081 tacgtgagca tcaacgagca ccgccccggc gtcgagcaca tcacccggac cgtgctcggc
     7141 gccggcttcg acgagatcgt gttcgtcgac gacgtcagcc gggtggccga ggacgcccgc
     7201 gcgcacggcg ccgggttcgt cgggttcccg tcgagccccg cccacgcgcg ccagcgggac
     7261 ttcatggccg gccacggcgt ccgccacttc gcggcgtcgc tcgccgaatt gaccccggaa
     7321 ctcctggaga tcgtcgacgc ggagctcgcg acttcgacac actggcttcg ttgaggattc
     7381 cccgcaccct tggagccgcc gtgaagtact tttcgctgcc ccgtcccggc gccgggctcg
     7441 tctcgggcga gcgcgacgtt cccgaacccg gtcccgggca ggtcctggtc cggctgcgcg
     7501 ggtgggcggt caacgcccgc gacctgatga tcgtcaaggg tttctacccg aagccggtga
     7561 aaccggacgt cgtgccgctg tccgacggcg cgggtgaagt cgtcgcggtc ggcgagggcg
     7621 tgcgtgcctg ggccaccggc gaccgggtgg ccgcgaccta cttcccggac tggctgtccg
     7681 ggcccggcac gccggagaag accgccgacg acctggcggg caccctcgac ggcgtcctcg
     7741 ccgagtacgc cgtcttcgcc gaggacgcgc tcgtcggcgt cccggcgcac ctcgactacg
     7801 ccgaagccgc gacactgccc agcgcgggcg tcacggcgtg gcgcgcggtc gtcgaggaag
     7861 gcgggctcgt gccgggccag accgtgctca ccctgggcag cggcggcctg tccaccttcg
     7921 cgctccagtt cgccgcgctc gggggcgcgc acgtcgtgtc gacgtccggc tcggacgaga
     7981 agctcgagcg gctccgcgag ctgggcgccg ccgagacgct caaccacgcg acgacgccgg
     8041 agtggggtgc cgcggtggcc cagctgaccg gcggcggcgt cgaccacgtc gtcgacgtgg
     8101 gcggcggcgg cacgatcggg cagtcgatgc tcgccgcgcg ctacggcggg cacgtcagcg
     8161 tcgccggcgt gctgacccac gaaggcggcg ccgacccgat cctcgtgctg gtcaagcagc
     8221 tgaccctgcg cggtctgacc aacgcctccc gtgagacgtt ccaggcgatg aaccgggcga
     8281 tcgagcacac cgggctccgg ccggtgatcg accgccggtt cgccttcgac gaggtcgaag
     8341 aagccctcaa gcacctcgag tccggtagcc acgtcgggaa ggtcgtgctc gaaagcgcct
     8401 gagcggcccc gggccccggg taacttttgc cgtcccggct gggtatttgt gtaccccgga
     8461 tcgttctttt cgcaggtcag cgggggtgtg cccgggcaaa aaagtcgggt ttcggcagga
     8521 atggcggctg tcggcggcac cggggagagt ggtgttcacc aggacatcgc ccgagcaggc
     8581 tgcggctgct caggcggaac gtccgggact gggggcgcgg ggagggggtt tcaaccgcct
     8641 gggggtgggt tgaaaccccc ttcccgcccg cgcccccccc ggcgggctca ggaagtggtg
     8701 caggcccggc cgttgagcga gatcgccgtg ggcctggtgt tcgccgtgcc ggacgtgccg
     8761 gtgaacccga actccgcgct gccgcccggc gggatcgtct tgttccagcc cgcgtccacc
     8821 gccgacaccg ccgtgccggt ctgggtgacc tgcgcgttcc acgcctgcgt cacgcgctgg
     8881 ccgtccgcga acgtccacgc caccgtccag ccggccaccg cggccgtgcc ggtgttgccc
     8941 accttgaccg aggcctggaa cccgccctgc cactggttcg tgaccgtgta ggccgccgtg
     9001 cacgaacccg acggcaccgg ggtggtcgtc gtcgtgggcg gtgtggtcgg cgtcgtagtg
     9061 gtggacgtgg tggtggtcgt cggcggtggc tcggtgcccc cggagtcgcc cacgatcacg
     9121 ccgcgcccgt tcgtgccgac gtagacccgg ccgtacaccc gcgggtcgcc gctcagcgcc
     9181 gcgccgatgt tgccgtactg gtgccggtcg tcgttgatcc gcgtccacga tccgcccgcg
     9241 tcgtccgacc ggaagatccc gcgcacgccc ccgatcttcg cgatcgtgta taacgtcgca
     9301 tacgtccggc cggtggcggc cttgccgaac ccgatgttgt cggcctcggt gaccccgctc
     9361 agcttcgtga aggacgctcc ggagtcggtc gagtgccaga ggccgtacgc cgtgccggag
     9421 ccgccggcga gccagacgtc gccggtcacc ccgggcatcg ccttgaagtg cgcggagccg
     9481 gtgggcaggc cggtggccgc ggtcgcggtg aaggtcgcgc cgccgtcggt gctgacgtag
     9541 aacttcccgg cggcgaaccc gtagaaccgc ttcgggtcga cgcggtcggc ctcgacgacc
     9601 gcgccggccg ggatgcccgc ggacgccgtc cacgacgaac cgaagccgac cgagtagttc
     9661 accggggtgc cgtccgggct ccacacgaac cggctgccgt cggcggccgc ggcgaccgtg
     9721 ccgccgctcg cgccaccgga cggctccgcg ccctggaacc agttcttgcc gccgtcggtg
     9781 gagaaggcga tccggttgtc gttcggccgc gcgccgcggt cgagcgtgcc ggcgcggacg
     9841 atcgtcgccg gggtcagctc cgcgtagtcg aggctcgcgg tcgtggtgaa gaccggctgg
     9901 gtgtacatgg tggccgggac ggcgtcgagg ctgtcgtgcc ggaacccgcc gatgtcgccg
     9961 agcccggaga gcagggcagc gcccgacggt gggctgatca ggtcgagcac cgcggtctcc
    10021 tcgaggccgc cgacgaccgg tttgagcgtg aacttcccgc cgctgtccca cttcgtgagg
    10081 tcggtcgtcc cgtagatcgt cgcgccggtg ccgtagagga agtggttcga gtcgaagggg
    10141 tcgatctccg cggactcggt catccagccc agcttcggcg acggcaccgg gggagcggcc
    10201 gggacgccga aggtcagcca cggcaccgaa gagatgtcct gggtgaaccg ctgcgatcgg
    10261 tcggggtagc tcgtgaagtc ccagatccgc gtccaggtgg cgccgccgtc ggtgctgcgg
    10321 aagaagatca cgtccggcca ccacgacacc tgcgtggcga ccatgagcgt gttcgggtgc
    10381 tggcggtcga tcgtcagccc gctgtagccg aagtagtcgt cggtgctgtc ggacgggatc
    10441 gggctgatcc gcgtccaggt accggtcttc gtggcgtact tccagacgtc gcccttcgcg
    10501 ccgtcgtagg ggccgccggt gtcgctggtc gcgaggtaga ggtagccgcc gaccgggtcg
    10561 aggacgccct tgtgcgccag gtacccggtg ggctggccgg ccaggcgcgc ccaggtcgtg
    10621 ccgccgtcgg tgctgcggta gacggtgttc tgcttgtccg cgacgccgac gtagatcgtc
    10681 tgcgtggtgc tgccgcgggt gccggtcgac gggtcgaacg tcacccaggt gacgccttcg
    10741 ttgtcgctgg agtagcccga cgggtcgctc gggtcgggcg cgtaggtgcc ggggttcggg
    10801 aaggcggtca ccttcgccca ggtgacgccg gagtcgg
//
