LOCUS       NZ_CP095376            10858 bp    DNA     circular CON 06-NOV-2024
DEFINITION  Amycolatopsis sp. FBCC-B4732 chromosome, complete genome.
ACCESSION   NZ_CP095376
VERSION     NZ_CP095376.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis sp. FBCC-B4732
  ORGANISM  Amycolatopsis sp. FBCC-B4732
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 10190959)
  AUTHORS   Choi,A.
  TITLE     Complete Genome Sequence of Amycolatopsis pretoriensis strain
            FBCC-B4732
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10190959)
  AUTHORS   Choi,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2022) Bioresources Collection & Research Team,
            Nakdonggang National Institute of Biological Resources, Donam 2-gil,
            Sangju-si, Gyeongsangbuk-do 37242, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 75.1x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023008405.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 10:39:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,364
            CDSs (total)                      :: 9,298
            Genes (coding)                    :: 9,177
            CDSs (with protein)               :: 9,177
            Genes (RNA)                       :: 66
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 50 of 121
            Pseudo Genes (incomplete)         :: 81 of 121
            Pseudo Genes (internal stop)      :: 14 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:14:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1126645
            Orig. end                         :: 1137503
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095376.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from NNIBR.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10858
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="7"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10858
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10858
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5858](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5858
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(36..659)
                     /locus_tag="MUY14_RS04445"
                     /old_locus_tag="MUY14_04445"
     CDS             complement(36..659)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682052.1"
                     /locus_tag="MUY14_RS04445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_04445"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_247021071.1"
                     /transl_table=11
                     /translation="MSWLADTRTSYDTVAESYAEFVAGALEEQPYLKAALTLFAAEVDG
                     PAVDVGCGPGHFTAYLASLGVDASGVDLSPAMVGLARRAHPELAFEVGSMTDLALPDAS
                     VAGVLASWSLIHVPDDDVPVALGHFRRVLRAGGLLVIGYHVGAGTRLKTEGYGGHPMRV
                     HIHLRQPWWLARRVRAAGFTVEAEWSLNPENKVSQAILFARASA"
     gene            complement(656..1198)
                     /locus_tag="MUY14_RS04450"
                     /old_locus_tag="MUY14_04450"
     CDS             complement(656..1198)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228115.1"
                     /locus_tag="MUY14_RS04450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_04450"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_247021073.1"
                     /transl_table=11
                     /translation="MTVGERPLRADARRNREALVAAAREVFGAKGVDAPLDEVARRAEV
                     AIGTLYNRFPTRADLVEAAFLPTLEEAEAITEEALRCADPWDGFVLLLERCVLMQVADR
                     GFTEVCSRAFDPASGIEKAKHANASRMNRIIERAQEAGALRTDFRGPDLAIVFAAATAT
                     PDWRRALGMVLDGLRAR"
     gene            1366..1596
                     /locus_tag="MUY14_RS04455"
                     /old_locus_tag="MUY14_04455"
     CDS             1366..1596
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003084005.1"
                     /locus_tag="MUY14_RS04455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_04455"
                     /product="hypothetical protein"
                     /protein_id="WP_247021075.1"
                     /transl_table=11
                     /translation="MHSDLSPAGRTGVLLVGTRGQRGPGEVLIRIRGGLETFLAWSDTE
                     LPKGTTVLVVETRGRRTVDVVAWDASDPASQ"
     gene            1613..2761
                     /locus_tag="MUY14_RS04460"
                     /old_locus_tag="MUY14_04460"
     CDS             1613..2761
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646016.1"
                     /locus_tag="MUY14_RS04460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_04460"
                     /product="flotillin family protein"
                     /protein_id="WP_247021076.1"
                     /transl_table=11
                     /translation="MFGYRVPAPNEAMLISGGKHSQGTSPFRVVTGHGAFVMPVFRKVR
                     FLTLSMCEAEVTEVCVTKQAIALTVRAVIAFKVGNDTESIVNAGQRFLSDQDQMSVLTG
                     RIFAGHLRSIIGSMTVEEIITERQKLATEVLDGSAVEMAKIGLSVDALQIQSIDDMKLG
                     YIAAMAAPHNAAIQRDAQIAQAVANQAAAEAEQKSQRTQAEYARQTSIVQAQYRAEVDK
                     AQAEAAQAGPLAQARAQQEVIDARTELAQREAELRQQQLVAEVIKPAGAEAERIRILAL
                     AEAEKMRVQAEAAASNNRVALDRMLIDQLPQIVKEAGRGLSGANVNILNGADGLGEMAA
                     GLVGQGLSILDSVKRGLSPSPSTPAAPEENGQVPKPAELTQS"
     gene            complement(2793..3824)
                     /locus_tag="MUY14_RS04465"
                     /old_locus_tag="MUY14_04465"
     CDS             complement(2793..3824)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019808441.1"
                     /locus_tag="MUY14_RS04465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_04465"
                     /product="TRM11 family methyltransferase"
                     /protein_id="WP_247021081.1"
                     /transl_table=11
                     /translation="MPEYAMLVYPSANRVYAASSTALLRAELAVFGAALAAELSAIDEV
                     ELGGVGYVRFTSSSPLAGRDLALLSNLSSLYALFELGDGVLRPVTVTPLAKADSDLLTI
                     QKYAGKTNELFTKLLVNVTLMATADPFSAKAKYLLDPLCGRGTTLNQAMMYGLHATGLD
                     VDGKDFEAYEAFIKTWLRNKRVKHTAESGQLRRNKVRLGRRLDIEYALSKEEYKAGDTR
                     RLVYLNADTLTTDEALRANSFDVIVTDAPYGVQHGSHREAGLQRSPRDLLAAAVPVWTR
                     VLRPGGALGISWNTTVLPREELVEVLRKAGLDVREGGPWEEFAHRVDQAILRDLVVAVK
                     PAS"
     misc_feature    3840..3853
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.75"
                     /tool="antismash"
     gene            3850..4839
                     /locus_tag="MUY14_RS04470"
                     /old_locus_tag="MUY14_04470"
     CDS             3850..4839
                     /EC_number="3.1.3.7"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /GO_function="GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228112.1"
                     /locus_tag="MUY14_RS04470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_04470"
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="WP_247021083.1"
                     /transl_table=11
                     /translation="MTPTSAQDIRDAVALLAAATDVTILGHVRPDADALGSALALGRAL
                     VQRGVKVRVSFGEPDEAPETLRWLDEDGLLTHPDDLPASEPLLICVDTPTPKRLGRLAG
                     RVEAAGAVLVVDHHATNTYYGTCHVVDESAEASAILVFRILDALGAALDEPIARGIYAG
                     IVTDTSGFRRASPETHRMAARLLEAGVDAEAVVRRIVDERPFAWLPMLSSVLNEARLEP
                     EAAQGKGFVHAVVRLDAAATVRGEEVESVIDVIRSVREAAVAAVLKQDPTNPAQWQISL
                     RSTGIDVSAVAAEFGGGGHRQAAGCTIPGTAEEVLAGLRAALERAPLL"
     gene            5001..5858
                     /locus_tag="MUY14_RS04475"
                     /old_locus_tag="MUY14_04475"
     CDS             5001..5858
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MUY14_RS04475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MUY14_04475"
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_247021084.1"
                     /sec_met_domain="AfsA (E-value: 5.2e-06, bitscore: 25.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEAKPLTGPVRKTGPGWTAGLTVPREHPFFFDHPLDHVPGSLTI
                     CGLLDLAESVTGPAGGAERLLLDLRFPAMGTLSGPVELGLAPAPGVPGQWSLLAQQADA
                     DICAGTLRRVAGLGRPATAPQPGDARNSLCPQDLVHRVDEDNIVLGVPADAGDEVTAPA
                     VPPRPGHYLDHGGPAFSARSLIESSRQFFTVLEHWAAGWSYGTTVLWTAFHADLPASPP
                     SPAYSFRWVREPVKRSKLDVSFDLLDAEGVVAGRFDYVAITVSAAAYERFRAARGRLAG
                     EAAA"
     gene            5855..6499
                     /locus_tag="MUY14_RS04480"
                     /old_locus_tag="MUY14_04480"
     CDS             5855..6499
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016107.5"
                     /locus_tag="MUY14_RS04480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_04480"
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="WP_247021085.1"
                     /transl_table=11
                     /translation="MIGLVRVNQVPRLLYFVIVGGLGAGLVLRLLSAADPRSAVAAGFV
                     LVHLVWIVAESRITFTPNGTSPPDPTVLPYGFARTVVVVAGALGPLPWAGGPLWWTPAP
                     AALFLGGIALRLRAIGELGRFYTHRVLRHDGHEVVTTGPYRAVRHPAYTGMLAASAGYT
                     AYLFTVPGAVALVVLAGALVWRIRVEERMLFDVPGYPAFAAGRARLVPGVW"
     gene            6502..9111
                     /locus_tag="MUY14_RS04485"
                     /old_locus_tag="MUY14_04485"
     CDS             6502..9111
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PPDK_N"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013491.5"
                     /locus_tag="MUY14_RS04485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_04485"
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="WP_247021086.1"
                     /sec_met_domain="PPDK_N (E-value: 5e-46, bitscore: 156.5,
                     seeds: 105, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLLHDGPRLTDPGVVGNKFARQAELRAAGFEVPEFFCVPVEAFD
                     AALARVPDGEPAARRRALLGHGVPEPLAGALLAAFDRLAGPDGVVAVRACVVGDGEDSA
                     ADPFAGMSDSFLNVGRAALLARVAECWASAFTDRAVAYRRLRGADPGAARVAVGVQRMV
                     RAERSFVAFSRDPRTGAAQHLIAAAYGLGEGVVQELADVDHFVHDPAAGRVSRTVVPKH
                     RMVVPDPARSGAPLLAGVPVGLVAAPVLTDAEVREIGVLAERVAEHFGAPQDIEGALTA
                     DGTIELVQARPIVFTEPEPDPGPASDVIWTNHNLTESFPGVTCALTYSQAQEFYEVCFR
                     GFYRRIGVPARLRHRHAGDLARMIGLLDGRVYYQLDAWYRLHAMMPGWGLLRPTWQRTL
                     GLSGDELDEAPPLPRDGRLRTVRALPRAVASLLGHPLAVRRFLTWWDDLAATTGPLDGL
                     PPAELVRRYRRIWAEFAGQWPVTLLNGYFLLGCLTLTQRLLSRWTTGDPALLVGLLPGG
                     PENRSLLALRSALRLATRFRHDPRLTAALAAGTDREVHDGIVAGRFGDDVAEAFAEHLR
                     RYGDRTVHDLKLEVRTPRQDPWRLIGVLRPLVASGETAEGNRTAERNARRDGVRRLRRA
                     CSSPVRRIALGLCVRVLRRFVKAREDTRLCRSQLYGLSRDILYRLGDVLAADGRLADAG
                     DVVHLRVEEVLGAVDGTGGTAGLADLAARRRAELEAWQGKPAPPGYLSPDGPGRDAARS
                     GPPPGNVLRGLASSRGVVRARAAVVLDPTAPVEDCRDRILVARETDPGWLYLMTAAKGI
                     VVERGSLLSHTAITGRLLGIPTVVAVPGATTAIADGGWIELDGAAGTVRLLRTEEAG"
     gene            9108..10298
                     /locus_tag="MUY14_RS04490"
                     /old_locus_tag="MUY14_04490"
     CDS             9108..10298
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012296.5"
                     /locus_tag="MUY14_RS04490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_04490"
                     /product="cytochrome P450"
                     /protein_id="WP_247021087.1"
                     /sec_met_domain="p450 (E-value: 5.4e-17, bitscore: 60.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAPEFPFTEGPGLEPPAEYGEILGAGALCPVTLAGGRHAVLVTR
                     HADVRTVLGDPRFSREDHGALFDREAGSLALLLTDPPVHTRRRRTVAAGFTARQAEAAR
                     PGLAALAAELVAGLRARGTEAELVEEFAVPFTLAVISDLLGVPAADRGRFRPWVNAMMS
                     RGHASPEEVAEGHRAMHEYFTALVDEIWAGDTRDGIIADLAPVPGRAGELSREEAIVMS
                     AGLLVAGYESTSNQLASCVYLLLTERARWESLLGEPAALDRAIEEMLRWTSFTTGGGTP
                     HVATEDVRLTDRLVRRGEVVVPLTDAANRDAEVFADPDRLDLTREPNPHVAFGAGRHRC
                     LGAELARAELQTGLAVLLRELPGLELAVPEGALRWRREMQVSGVWELPVRWAGDAR"
     misc_feature    10455..10470
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.14"
                     /tool="antismash"
ORIGIN
        1 tcctgccgga cgtcctggat ttcgtgggac agcggctacg ctgacgcgcg ggcgaacaga
       61 atcgcttgcg acaccttgtt ttccgggttc agcgaccact cggcctcgac cgtgaacccg
      121 gccgcgcgca cccgcctcgc cagccaccac ggctgccgga ggtggatgtg gacgcgcatc
      181 gggtgcccgc cgtagccctc ggtcttcagc cgggtcccgg cgccgacgtg gtacccgatc
      241 accagcagcc cgcccgcgcg cagcacgcgc cggaagtgcc ccagcgccac cgggacgtcg
      301 tcgtccggga cgtggatcag cgaccaggac gcgagcacgc ccgccaccga cgcgtcgggc
      361 agcgccaggt ccgtcatcga gcccacttcg aacgccagct ccgggtgcgc ccgccgggcc
      421 agcccgacca tcgccgggga gaggtcgacg ccggacgcgt cgacgccgag cgaggccagg
      481 tacgccgtga agtgccccgg cccgcagccg acgtccaccg ccggcccgtc gacctccgcg
      541 gcgaacagcg tcagggcggc cttgaggtac ggctgctctt cgagggcgcc ggccacgaac
      601 tcggcgtagc tctcggcgac ggtgtcgtag gaggtgcggg tgtccgcgag ccagctcacc
      661 gcgcgcgcag cccgtcgagg accatgccga gcgcccgccg ccaatccggg gtggcggtcg
      721 ccgcggcgaa gacgatggcg aggtcgggcc cgcggaagtc cgtgcgcagc gccccggcct
      781 cctgcgcccg ttcgatgatc cggttcatcc gggaagcgtt ggcgtgcttg gctttctcga
      841 tccccgaggc cgggtcgaac gcccgcgagc acacctcggt gaacccgcgg tcggcgacct
      901 gcatgagcac gcagcgttcg agcaggagca cgaagccgtc ccagggatcg gcgcaccgga
      961 gcgcttcttc ggtgatggct tcggcttctt cgagcgtcgg gaggaaggcg gcctcgacga
     1021 ggtcggcccg ggtggggaac cggttgtaga gggtcccgat ggcgacctcg gcccgcctcg
     1081 cgacttcgtc gagcggggcg tcgacgccct tggccccgaa gacttcccgc gcggcggcga
     1141 ccagggcctc ccggttgcgc cgcgcgtccg cgcgcagggg acgctcgccg acggtcacgc
     1201 tccggagtat agccaagccg gacgtcagta cacgcccgag cgggctgccc ggttgcctcc
     1261 tgatcggaac ctcacagtgc ccaccgccgg tggcggccag gagcacaatg gggttcagcg
     1321 ggggcgcaac ccgcaccgag gcggtcagcg agggaggtcg gcgccatgca ctccgacctc
     1381 tccccggcgg gccggaccgg agtcctcctc gtcggcaccc gcgggcagcg gggcccaggg
     1441 gaggtgctga tcaggatcag gggcggcctg gaaaccttcc ttgcctggtc cgacacggag
     1501 ctgccgaagg gcacgaccgt cctggtcgtg gaaacccggg gacggcgcac ggtcgacgtc
     1561 gtggcgtggg acgcctcgga tcctgcttct cagtgatcgc ggaaggacag ccgtgttcgg
     1621 ttatcgcgtt ccggccccca acgaggcgat gctcatctcc gggggcaagc acagccaggg
     1681 cacctcgccg ttccgggtcg tcaccggcca cggcgcgttc gtcatgccgg tcttccggaa
     1741 agtccggttc ctcacgcttt ccatgtgcga ggccgaagtg accgaagtgt gcgtcacgaa
     1801 acaggccatc gcgctgacgg tccgggcggt gatcgcgttc aaggtcggca acgacaccga
     1861 gagcatcgtc aacgccgggc agcgcttcct ctccgaccag gaccagatgt ccgtgctgac
     1921 cggccggatt ttcgccgggc acctgcggtc catcatcggg tcgatgacgg tcgaggagat
     1981 catcaccgag cggcagaagc tcgcgaccga ggtgctggac ggctcggcgg tcgagatggc
     2041 gaagatcggg ctcagcgtcg acgcgctgca gatccagtcg atcgacgaca tgaagctcgg
     2101 ctacatcgcc gcgatggccg ccccgcacaa cgccgccatc cagcgggacg cccagatcgc
     2161 gcaggcggtg gcgaaccagg ccgcggcgga ggcggagcag aagtcccagc ggacgcaggc
     2221 ggagtacgcg cggcagacgt cgatcgtgca ggcgcagtac cgcgccgagg tcgacaaggc
     2281 gcaagcggag gcggcgcagg ccggcccgct cgcgcaggcg cgggcgcagc aggaggtcat
     2341 cgacgcgcgc accgagctgg cgcagcgcga ggcggagctg cgccagcagc agctggtggc
     2401 cgaggtcatc aagcccgccg gcgccgaagc cgagcggatc cgcatcctgg cgctggccga
     2461 ggcggagaag atgcgcgtac aggcggaggc ggcggcgtcg aacaaccggg tcgcgctcga
     2521 ccggatgctc atcgaccagc tgccgcagat cgtcaaggaa gcgggccgcg ggctgtccgg
     2581 cgcgaacgtc aacatcctca acggcgcgga cggcctgggc gagatggccg cgggactcgt
     2641 cggccaggga ctgtccatct tggactcggt gaagcgcggc ctgagcccgt caccgtcgac
     2701 gccggcggct cccgaggaga acggtcaggt gccgaagccg gccgagctca cgcagtcctg
     2761 aggcgctttg tcgactcagg atgcttttcg gctcaggagg cgggtttcac cgcgacgacc
     2821 aggtcgcgca ggatggcctg gtcgacccgg tgggcgaact cctcccacgg gccgccctcc
     2881 cggacgtcga ggccggcctt ccgcagcacc tcgaccagct cttcgcgcgg cagcaccgtg
     2941 gtgttccacg agatccccag cgcgccgccc gggcgcagca cccgggtcca caccgggacc
     3001 gcggccgcca ggaggtcgcg cgggcttcgc tgcaggcccg cttcgcggtg gctcccgtgc
     3061 tgcacgccgt acggcgcgtc ggtcacgatc acgtcgaacg agttcgcccg cagcgcctcg
     3121 tcggtggtca gcgtgtccgc gttcaggtac acgagccgcc gggtgtcgcc ggccttgtac
     3181 tcctccttgg acagcgcgta ctcgatgtcg agccgccggc cgaggcggac cttgttccgg
     3241 cgcagctggc ccgactccgc ggtgtgcttc acgcgcttgt tccgcagcca cgtcttgatg
     3301 aacgcctcgt acgcctcgaa gtccttgccg tcgacgtcca ggccggtggc gtgcaggccg
     3361 tacatcatcg cctggttcag cgtcgtgccg cggccgcaca gcgggtccag caggtacttc
     3421 gccttcgcgg aaaacgggtc ggccgtcgcc atcagcgtca cgttcaccag cagcttcgtg
     3481 aacagctcgt tggtcttgcc cgcgtacttc tggatcgtca gcaggtccga gtccgccttc
     3541 gccagcggtg tcacggtgac tgggcgcagg accccgtcgc ccagctcgaa cagcgcgtac
     3601 aacgaggaca ggttggacag cagcgcgagg tcgcgcccgg ccagcgggga cgaactcgtg
     3661 aagcgcacgt accccacgcc gccgagttcg acctcgtcga tcgcggacag ttcggcggcc
     3721 aaggccgcgc cgaagaccgc cagctcggcg cgcaacaggg cggtggagga ggcggcgtag
     3781 acccggttgg ccgacgggta gaccagcatc gcgtactcag gcatcccgca gagcgtagcg
     3841 tgacgcaccg tgacccctac ttcggcgcag gacatccggg acgcggtggc gctgctcgcc
     3901 gcggccaccg acgtgacgat cctcggccac gtccgccccg acgcggacgc gctgggcagc
     3961 gcgctcgccc tcggccgggc cctcgtgcag cggggcgtca aggtgcgcgt ctccttcggc
     4021 gagcccgacg aggcgcccga gacgctgcgg tggctcgacg aagacggcct gctgacccac
     4081 ccggacgacc tccccgcctc cgaaccgctg ctgatctgcg tcgacacgcc gacgccgaag
     4141 cggctgggcc ggctcgccgg gcgcgtcgaa gccgccggcg ccgtgctggt ggtcgaccac
     4201 cacgccacga acacctacta cggcacctgc cacgtcgtcg acgagtcggc cgaggccagc
     4261 gcgatcctgg tgttccgcat cctggacgca ctgggcgccg cgctggacga gccgatcgcg
     4321 cgcgggatct acgccgggat cgtcaccgac accagcgggt tccgccgggc gagcccggag
     4381 acgcaccgga tggcggcgcg gctgctggaa gcgggcgtcg acgccgaagc ggtcgtgcgg
     4441 cggatcgtcg acgagcgccc gttcgcgtgg ctgccgatgc tgtcgtccgt gctgaacgag
     4501 gcccggctcg aaccggaagc cgctcagggc aagggtttcg tgcacgcggt ggtgcggctc
     4561 gacgcggccg ccaccgtgcg cggggaagag gtcgagtcgg tcatcgacgt catccgctcg
     4621 gtacgcgaag ccgccgtcgc cgccgtgctc aaacaggacc cgaccaaccc ggcgcagtgg
     4681 cagatctcgt tgcgctccac cgggatcgac gtctcggcag tcgccgcgga gttcggcggc
     4741 ggcgggcacc gccaggccgc gggctgcacg atcccgggca ccgccgagga ggtcctcgcc
     4801 ggactgcgcg ccgcgctgga gcgggcgccg ctgctctagc cgcacgcccg gcggacggtt
     4861 tcgtgcggaa cgcactgccc gtccgggaat tcggcacttc gttgcgcgct cgccgcaatc
     4921 ggggagcaag ccccgccgcg atcacgcgcg atgcttcgtt gccagccggt cttgtccggc
     4981 tgggaacgaa ggaggcgcgc atgagcgaag cgaagccgct gaccgggccg gtgcggaaaa
     5041 cgggtccagg gtggaccgcg ggactgaccg tgccgcggga acaccccttc ttcttcgacc
     5101 acccgctgga ccacgtgccc ggctcgctga ccatctgcgg cctgctcgac ctggcggaat
     5161 cggtgaccgg gccggcgggc ggggccgagc ggctgctgct cgacctgcgc ttcccggcga
     5221 tggggacgct gtccggcccg gtcgaactcg gcctggcccc ggcaccgggt gtgcccggcc
     5281 agtggtcgct gctggcccag caggccgacg cggacatctg cgccggcacc ctgcgccggg
     5341 tcgcgggcct cggccggccg gccaccgcac cgcaaccggg ggatgcgcgg aactcgctgt
     5401 gcccccagga cctcgtccac cgcgtcgacg aggacaacat cgtgctcggc gtcccggccg
     5461 acgccgggga cgaggtcacc gcgcccgccg tgccgccccg gccggggcac tacctcgacc
     5521 acggtggtcc cgctttctcg gcgcgcagcc tgatcgagtc gagccggcag ttcttcaccg
     5581 tgctggagca ctgggcggcg ggctggtcct acggcaccac ggtgctgtgg acggctttcc
     5641 acgccgacct gccggcgtcg ccgccgagcc cggcgtactc cttccgctgg gtccgcgagc
     5701 cggtgaagcg cagcaagctg gacgtctcgt tcgacctgct cgacgccgag ggcgtggtgg
     5761 ccggccggtt cgactacgtc gccatcaccg tcagcgccgc cgcgtacgag cggttccgcg
     5821 ccgcccgcgg ccgcctggcc ggggaggccg ccgcgtgatc ggcctcgtgc gggtgaacca
     5881 ggttccccgg ctcctgtact tcgtcatcgt cggcggcctc ggcgcgggcc tcgtgctgcg
     5941 cctgctctcg gccgccgacc cgcggtccgc ggtggccgcc gggttcgtgc tggtccacct
     6001 ggtctggatc gtcgcggaat cccggatcac cttcacccct aacggcactt cgccgcccga
     6061 cccgaccgtg ctgccctacg gtttcgcgcg cacggtggtc gtcgtcgccg gcgcgctcgg
     6121 cccgctgccg tgggccgggg gcccgctgtg gtggacgccg gcgcccgccg cgttgttcct
     6181 gggcggaatc gcgttgcggc tgcgggcgat cggcgagctc ggccggttct acacccaccg
     6241 cgtgctgcgc cacgacggcc acgaagtcgt caccaccggg ccgtaccgcg cggtccgcca
     6301 cccggcctac accgggatgc tggcggcgag cgccgggtac accgcctacc tgttcaccgt
     6361 gcccggcgcc gtcgcgctcg tggtgctcgc cggggcgctg gtgtggcgga tccgcgtcga
     6421 ggagcggatg ctcttcgacg tcccggggta tcccgcgttc gccgccggcc gcgcccggct
     6481 ggtcccgggg gtgtggtgac cgtggacctc ctccacgacg gcccgcggct gaccgacccc
     6541 ggcgtcgtcg ggaataagtt cgcccgccag gccgagctcc gcgccgcggg gttcgaagtc
     6601 cccgagttct tctgcgttcc ggtggaggcg ttcgacgccg ccctcgcccg ggtgccggac
     6661 ggcgagcccg ccgcccggcg gcgggcgctg ctcggccacg gcgtaccgga gccgctggcc
     6721 ggcgcgctgc tggcggcgtt cgaccggctc gccggcccgg acggcgtggt cgccgtgcgc
     6781 gcctgcgtcg tcggcgacgg ggaagacagc gcggccgacc cgttcgccgg gatgagcgac
     6841 agcttcctca acgtcggccg cgcggcgctg ctcgcccggg tcgccgagtg ctgggcgtcc
     6901 gcgttcaccg accgggcggt ggcctaccgg cggctgcgcg gcgcggaccc gggcgcggcg
     6961 cgggtcgcgg tcggcgtgca gcgcatggtg cgggcggaac gctcgttcgt cgcgttcagc
     7021 cgcgatccgc gcaccggggc cgcgcagcac ctgatcgcgg ccgcgtacgg gctgggcgaa
     7081 ggcgtcgtcc aggaactcgc cgacgtcgac cacttcgtgc acgaccccgc ggccggtcgc
     7141 gtgtcgcgca cggtcgtccc gaagcaccgc atggtcgtgc ccgatcccgc tcgctcgggt
     7201 gcgccgctgc tcgccggcgt cccggtcggg ctcgtcgcgg cgccggtgct gaccgacgcc
     7261 gaggtgcgcg agatcggcgt gctcgccgag cgggtggccg agcacttcgg tgccccgcag
     7321 gacatcgagg gcgcgttgac ggcggacggg acgatcgaac tggtccaggc ccgcccgatc
     7381 gtcttcaccg agccggagcc ggaccccgga ccggcgtccg acgtcatctg gaccaaccac
     7441 aacctgaccg agagcttccc gggcgtcacc tgcgcgctca cctactcgca ggcccaggag
     7501 ttctacgaag tctgcttccg cgggttctac cgccggatcg gcgtgcccgc ccggttgcgg
     7561 caccggcacg ccggcgacct cgcgcggatg atcgggctgc tcgacgggcg cgtctactac
     7621 cagctcgacg cctggtaccg gctgcacgcg atgatgcccg gctgggggct gctgcgcccg
     7681 acctggcagc ggacgctggg cctgtccggc gacgagctcg acgaggcgcc gccgctgccg
     7741 cgcgacggcc ggctgcggac ggtgcgtgcg ctgccccgcg cggtcgcgag cctgctcggg
     7801 cacccgctcg ccgtgcgccg gttcctgacg tggtgggacg acctcgccgc gaccaccggg
     7861 ccgctcgacg gcctcccgcc ggcggaactg gtccgccgct accgccggat ctgggcggag
     7921 ttcgccgggc agtggccggt caccctgctc aacggctact tcctgctcgg ctgcctgacg
     7981 ctgacgcagc ggctgctgtc ccgctggacc accggcgacc cggcgctgct cgtggggctg
     8041 ctgcccggcg gcccggagaa ccgctcgctg ctggccctgc gctcggcgct gcggctggcc
     8101 acgcggttcc ggcacgaccc gcggctgacg gcggcgctgg ccgccgggac cgaccgggag
     8161 gtgcacgacg ggatcgtcgc cggccgcttc ggggacgacg tcgccgaggc gttcgccgag
     8221 cacctgcgcc ggtacggcga ccggaccgtg cacgacctga agctcgaggt ccgcacgccg
     8281 cgccaggacc cgtggcggct gatcggcgtg ctgcggccgc tggtggccag cggcgagacc
     8341 gccgaaggca accgcaccgc ggagcggaac gcccgccgtg acggcgtccg gcggctgcgc
     8401 cgggcctgct cgagcccggt gcgccggatc gcgctcgggc tctgcgtccg ggtgctgcgg
     8461 cggttcgtca aggcgcggga ggacacccgg ttgtgccgca gccagctcta cggcctcagc
     8521 cgggacatcc tctaccggct gggtgacgtc ctggccgccg acggccggct cgccgacgcc
     8581 ggcgacgtcg tgcacctgcg ggtcgaagag gtcctcggtg ccgtcgacgg caccggcggc
     8641 acggccgggc tcgccgacct ggccgcccgg cgccgggcgg aactcgaagc ctggcaaggg
     8701 aaacccgcgc cgccgggcta cctctcaccg gacggacccg gccgcgacgc cgcccgcagc
     8761 ggcccgccgc cgggtaacgt cctgcgggga ctggcctcca gccgcggcgt cgtccgggcc
     8821 cgggcggcgg tcgtgctcga cccgaccgcc ccggtcgagg actgccggga tcgcatcctc
     8881 gtcgcccgcg agaccgatcc cggctggctg tacctgatga ccgcggcgaa gggcatcgtc
     8941 gtcgaacgcg gcagcctgct gtcgcacacc gccatcaccg ggcggctgct ggggatcccg
     9001 accgtggtgg ccgtgcccgg cgccaccacc gccatcgccg acggcggctg gatcgaactg
     9061 gacggcgcgg ccggcacggt ccggctgctg cgcaccgagg aggcgggatg agcgcgcccg
     9121 agttcccgtt caccgaagga cccgggctcg aaccgcccgc ggagtacggg gaaatcctcg
     9181 gtgccggcgc gctgtgtccg gtgacgctgg cgggcggccg gcacgccgtg ctcgtcacgc
     9241 ggcacgccga cgtgcggacc gtgctcggcg acccgcggtt cagccgcgag gaccacgggg
     9301 ccctgttcga ccgggaggcc gggtcgctcg cgttgctgct gaccgatccg ccggtgcaca
     9361 cccggcggcg ccgcacggtc gcggccggct tcacggcgcg gcaggccgag gccgcgcgcc
     9421 cgggactggc cgcactggcg gccgagctgg tcgccgggct gcgggcgcgg ggcaccgaag
     9481 cggaactcgt cgaggagttc gcggtgccgt tcacccttgc ggtgatcagc gacctgctcg
     9541 gggtgccggc cgcggaccgg ggccggttcc ggccctgggt gaacgcgatg atgtcgcgcg
     9601 gccacgcctc gcccgaagag gtcgccgagg ggcaccgcgc gatgcacgag tacttcaccg
     9661 cgctggtcga cgaaatctgg gcgggggaca cccgcgacgg catcatcgcc gacctcgccc
     9721 cggtgcccgg ccgggcgggg gagctctcgc gcgaggaagc gatcgtgatg agcgccggcc
     9781 tgctcgtcgc gggctacgag tcgacgagca accagctcgc gtcctgcgtc tacctgctgc
     9841 tcaccgaacg cgcccgctgg gaatccctgc tgggcgaacc cgcggcgctc gaccgggcga
     9901 tcgaggagat gctgcgctgg acgtcgttca ccaccggcgg cggcacgccg cacgtggcca
     9961 ccgaggacgt gcggctgacc gaccggctgg tgcgccgcgg cgaggtcgtg gtgccgctga
    10021 ccgacgcggc gaaccgcgac gccgaggtct tcgccgaccc ggaccggctg gacctgacca
    10081 gggagcccaa cccgcacgtg gccttcggcg ccgggcggca ccggtgcctc ggcgccgaac
    10141 tggcgcgggc cgagttgcag accggcctgg cggtcctgct gcgcgaactg cccggactcg
    10201 aactcgcggt gcccgaaggg gcgctgcgct ggcggaggga gatgcaggtg agcggtgtgt
    10261 gggagcttcc ggtgcgctgg gcgggtgacg cgcgatgagc gagcccgccg tccccgacgt
    10321 gtacttcacc gaccggcaga tcgcggcggt ccggcacgtg gtcgagcacc gcctcggcgg
    10381 cgagatcgaa ctggcctacc tggccgggag cctcgccgtc ggcctcggcc acgggacgtc
    10441 cgatgtggac gtctacgccg tcggcgacca cctgcccggc caggaccacg gctacgagca
    10501 cgacggcgtc gtggtgcacg tgaccaaact ggacggtgcg ctggtgcgcc ggctggccgg
    10561 gctcggcagc cggttcaccg ccaccggcgc cgaccgggag cagttcctgc tcgagttcaa
    10621 ggtgctcaac gcgctggtcc ggctgaccac cgggcaccgg gtcaaggtgg ccccggcctg
    10681 gcaggacgcg ctcgcgccgc tgcgccggga cgtcgtgcgg cggatcctcg tcgcccggca
    10741 cgccaacatc ttcgcggcct tcgccgagga cgtctggggc gcgttgctca gcggcgaccg
    10801 ctacaccgcg gcgacggcgt ccgcgatcgc gctggagaac gcggcggaag cgctgctg
//
