LOCUS       NZ_CP095376            11362 bp    DNA     circular CON 06-NOV-2024
DEFINITION  Amycolatopsis sp. FBCC-B4732 chromosome, complete genome.
ACCESSION   NZ_CP095376
VERSION     NZ_CP095376.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis sp. FBCC-B4732
  ORGANISM  Amycolatopsis sp. FBCC-B4732
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 10190959)
  AUTHORS   Choi,A.
  TITLE     Complete Genome Sequence of Amycolatopsis pretoriensis strain
            FBCC-B4732
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10190959)
  AUTHORS   Choi,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2022) Bioresources Collection & Research Team,
            Nakdonggang National Institute of Biological Resources, Donam 2-gil,
            Sangju-si, Gyeongsangbuk-do 37242, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 75.1x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023008405.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 10:39:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,364
            CDSs (total)                      :: 9,298
            Genes (coding)                    :: 9,177
            CDSs (with protein)               :: 9,177
            Genes (RNA)                       :: 66
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 50 of 121
            Pseudo Genes (incomplete)         :: 81 of 121
            Pseudo Genes (internal stop)      :: 14 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:14:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 629201
            Orig. end                         :: 640563
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP095376.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from NNIBR.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11362
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11362
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11362
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6362](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6362)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(13..29)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.28"
                     /tool="antismash"
     misc_feature    132..146
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.57"
                     /tool="antismash"
     misc_feature    complement(134..145)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: strong, score: 20.32"
                     /tool="antismash"
     gene            complement(187..780)
                     /locus_tag="MUY14_RS02520"
                     /old_locus_tag="MUY14_02520"
     CDS             complement(187..780)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513297.1"
                     /locus_tag="MUY14_RS02520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_02520"
                     /product="dihydrofolate reductase family protein"
                     /protein_id="WP_247020378.1"
                     /transl_table=11
                     /translation="MARVVVHAVVSLDGYIAYPDDSVGPLFDWYSNGDVTIRANDEWSF
                     NVSQASADYVQPFWDAIKVTVIGRHLFDTFDGWGGKPASADELVVVTHRPLPDEWLARF
                     PDAPFHTADSIENGINLAKKLAGDGLVCVAAGDVAGQAFSAGLVDEVALDVAPVVFGEG
                     KRYFGSHAGTVQLEDPDVVIQGDRVLHLHYNVKR"
     gene            complement(868..1335)
                     /locus_tag="MUY14_RS02525"
                     /old_locus_tag="MUY14_02525"
     CDS             complement(868..1335)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670349.1"
                     /locus_tag="MUY14_RS02525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_02525"
                     /product="VOC family protein"
                     /protein_id="WP_247020380.1"
                     /transl_table=11
                     /translation="MSGAAGGGVRRFEHVGVIVDDLEAVTAFFADLGFEVAAPMRIEDE
                     WVERILGLEGVRLEMVMVNAPDGSGKLELTKFHHPVDPATDSADAQAPAVNRLGFRHIA
                     YIVENLDSTIERIRAKGLDTIGEIVNYENIFRMCYVRGPEGLIVELAEESN"
     gene            complement(1382..1999)
                     /locus_tag="MUY14_RS02530"
                     /old_locus_tag="MUY14_02530"
     CDS             complement(1382..1999)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /locus_tag="MUY14_RS02530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_02530"
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="WP_247020382.1"
                     /transl_table=11
                     /translation="MDLPTLLERAFRTTADVVAAIEPAAWNGPSPCQRWSVRQVGNHML
                     GMVGLATQAATGPALDPADSQPDRLEHLAHTERLGADPAATVRALGDRCVTAFSEPGIL
                     DRVTPGKGGADTPGSVHALMCLNEVLTHGWDLASGGGIAYAPDAEVVAAAQKFAAVLVG
                     PEARALGMFGPPVEVGADADLLTAHLGHVGRRSPWPGAQAAA"
     gene            complement(2154..2735)
                     /locus_tag="MUY14_RS02535"
                     /old_locus_tag="MUY14_02535"
     CDS             complement(2154..2735)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MUY14_RS02535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MUY14_02535"
                     /product="hypothetical protein"
                     /protein_id="WP_247020384.1"
                     /transl_table=11
                     /translation="MIGPTPEQLLLLGVLPQWTVAARIPPQRAKVRTTHRASGQVAQPD
                     RAEFQSRQPCYPGGEPGRRVSRATERSHRAMKPVNPTRRTDPAHPLEDTIVADHGSTPR
                     HRRRRENGAARAIFAEARRAGLVQRHLRKLHHLTFRAAAEHSGTAPPVAGGTALSSSDP
                     TTGGLAAPCGDTLRTANDPVPDQAAANRAA"
     tRNA            complement(2782..2858)
                     /anticodon=(pos:complement(632023..632025),aa:Arg,seq:tct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="MUY14_RS02540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="MUY14_02540"
                     /product="tRNA-Arg"
     gene            complement(2782..2858)
                     /locus_tag="MUY14_RS02540"
                     /old_locus_tag="MUY14_02540"
     misc_feature    complement(2827..2845)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.51"
                     /tool="antismash"
     misc_feature    complement(2989..3001)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 20.33"
                     /tool="antismash"
     gene            complement(3227..3610)
                     /locus_tag="MUY14_RS02545"
                     /old_locus_tag="MUY14_02545"
     CDS             complement(3227..3610)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645549.1"
                     /locus_tag="MUY14_RS02545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_02545"
                     /product="peptidase inhibitor family I36 protein"
                     /protein_id="WP_247020386.1"
                     /transl_table=11
                     /translation="MSSSRLRSRLPQLLVLAVLGLLTSTGVAQAASPPPDPACQKGEFC
                     LWSADAYGGESQRFDLRTANPGECIPLPEGFAGSSFANLMSRDVTVYQDEECSTEGDFI
                     TYPGGGTFVPNAPFLVRAVQIWE"
     gene            3827..5029
                     /locus_tag="MUY14_RS02550"
                     /old_locus_tag="MUY14_02550"
     CDS             3827..5029
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783737.1"
                     /locus_tag="MUY14_RS02550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_02550"
                     /product="MFS transporter"
                     /protein_id="WP_247020388.1"
                     /transl_table=11
                     /translation="MRDRVAVFTVFALNGLAIGSWATRTPALAAQVHASPGVFGLALLG
                     ASVGLLSAASVAGRVVERLGARAVVAGTTVLAAVALVLIGYAPGVPLLAGALFVVGASV
                     GMLDVAMNVAAVAVERRARRPVMPVFHAGFSVGALAGSLAAGLAAGHGWSPARHLTTAA
                     VVAVVVLLVVVRAVPGSRPEHAAAVTGRRAPIRRPVLWLLAAVALCSAVAEGAAADWSA
                     LLMTAERGVGQGAAALAFAGFQLVMALARLAGPWAQRRFGPTRCLVAGAALATAGFLAT
                     ATVPAAVVAYAGFALAGAGLAASFPLALSLAGDAGKRGDGAGGEREIGFVSAVAYAGFL
                     AGPPIIGGIAQAVGFGAAFGFVALVAALILPAAVAARRSRRREAAGQPAPEVTRGASDP
                     G"
     gene            complement(5001..6362)
                     /locus_tag="MUY14_RS02555"
                     /old_locus_tag="MUY14_02555"
     CDS             complement(5001..6362)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03604"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03604.1"
                     /locus_tag="MUY14_RS02555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_02555"
                     /product="YcaO-like family protein"
                     /protein_id="WP_247020390.1"
                     /sec_met_domain="TIGR03604 (E-value: 1e-66, bitscore:
                     224.4, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="YcaO (E-value: 3.5e-59, bitscore: 200.3,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTALHEGLPGRSGERRSAPGSGPIVTRLRSFAGLAGTPAALHLAV
                     ADIRDVRAELPWQGDLQGFGTSWDDPAHARVSARGEAVERYCATRPPRDVVHGSHDDLV
                     ARGLTALSPGRLALYSPRQYATPGFPFTPFLPSSPVHWVRATSLGGGDVHVPAFLVHTG
                     WPHVPKASEEPLHAFPAIGGTAAGETLEAAIRSGLQEIVEHDASAVWWLNGAPQPTLAP
                     TPRLASLAGADEVRFWSIANAFGVPVLAAAVRSPEGWLTVGTATRPDPEQAALKALAEA
                     YTLQITCRTLDDRDALSAIIAGYGGRPGPLRPWRAERDYADHYGPADLVELLCHQQFHL
                     DPRAGAAALAWATGGPPLDWAAIPRPADDSLDALLACVAAAGHEVLYVDLTTPEAAADG
                     VRVVRVLVPGTVGTAPAAYPPLGLRRVQDLGVRLGWHPEPLDEEQLNRDPMPHG"
     gene            complement(6362..6508)
                     /locus_tag="MUY14_RS02560"
                     /old_locus_tag="MUY14_02560"
     CDS             complement(6362..6508)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MUY14_RS02560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MUY14_02560"
                     /product="hypothetical protein"
                     /protein_id="WP_158104371.1"
                     /transl_table=11
                     /translation="MKQEQFSVETAPEALVVNTCVAASLVVNTCVAASLTVNTCVAAYL
                     PER"
     gene            complement(6505..7725)
                     /locus_tag="MUY14_RS02565"
                     /old_locus_tag="MUY14_02565"
     CDS             complement(6505..7725)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1202: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.5"
                     /locus_tag="MUY14_RS02565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_02565"
                     /product="MFS transporter"
                     /protein_id="WP_247020392.1"
                     /transl_table=11
                     /translation="MFWWIWISLLVNWAGAFTRPVLALSLTAGHGYPVSYAGFVIALIG
                     LGGVLGTVAGGVLADRLGRRPTLVLAHVVTAVAMVALGAASGRWQLEAAAFAVGVGSVA
                     ARPAMNAALADVVPPADRPRAFSLNYWAVNVGVAVSAALTGVMVGFGYPVVFIADAAAT
                     ALCALVVLLKVPETRPPFTPRARVARPPLRFDLRFAVFLLATLAIGSVYEQSATTLPVV
                     MVGAGLTAGAFGLVQALNGLLIAFLQLPLTRWARRHRRRPLLVASALLIGWGFGLNFFA
                     EGLLGYAVIALVWTVGEMLQMPVGSSVAADRAPSTQRGRYAALYTGAWSAAALLGPAGG
                     GWLLDRGGPAAVWLACAALGALAAAGFAVGTKLPREVAASDARNGEHSPDPATGTGGGP
                     SMERVAP"
     misc_feature    7703..7716
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.41"
                     /tool="antismash"
     gene            8151..9314
                     /locus_tag="MUY14_RS02570"
                     /old_locus_tag="MUY14_02570"
     CDS             8151..9314
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014467529.1"
                     /locus_tag="MUY14_RS02570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_02570"
                     /product="sensor histidine kinase"
                     /protein_id="WP_396126833.1"
                     /transl_table=11
                     /translation="MLLGGAGALAGGSGRWGALSLVQGAVPRLVLVPVVFRLVLFLQAL
                     WAAGGRTAPVLWLAVAFFLVPNLLEVAWVFRRATGIRPVLVADTAYTLVTSLAAAAFAP
                     GLLGLTWPNIMGTVMVWTLLRGAPAGAAAIAGAVLLRYGTAAVSGTSAPATWILPALLA
                     AAATALAIVLLVAGSMRFAHRLGEQRGRAAERERHRRDVHDTVLQVMESLALPAPDDAL
                     DPVGRLDQVRRTARAHALRLRLSLDGDAPAEPGGLEHRLGALAVEMTADGLRVDLVVLA
                     KPSDVPEAVVNALHDAAREALRNTLKHAKTARAVVCLEEREGVITVSVRDHGTGFDPGA
                     RRAGFGIDNSIHARLAEVGGAARIDSAPGQGTRVVLTAPLELRFPVG"
     gene            complement(9288..9926)
                     /locus_tag="MUY14_RS02575"
                     /old_locus_tag="MUY14_02575"
     CDS             complement(9288..9926)
                     /EC_number="5.4.2.-"
                     /GO_function="GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1101: phosphoglycerate mutase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228601.1"
                     /locus_tag="MUY14_RS02575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MUY14_02575"
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="WP_247020396.1"
                     /transl_table=11
                     /translation="MKMILLRHAESLGNVDELAYTRIPDHALPLTEKGEREALAAGPEI
                     ARLLDGERPAVYVSPYLRTRETLRLLDIEASCDRLLQEPRLREQDWGNLQDPADQEVQK
                     ARRNEFGHFFYRLPFGESGADVDDRVAAFLSDLRLRDERHPETVLIVSHGLTLRLLCRR
                     LFGWSIELFESLSNPGTCEYRVLEEQDGKWTLDRPFAQWRDSPDGETQL"
ORIGIN
        1 accagtcagc catgcttgcc aagctacaag aaccgccggt cggcgggcgt atccgaacgt
       61 gcgggaaggc ccgcgcgcgc gactgcgccg aggcacgcag atcgccgaga gcggccgtgc
      121 tcccggcgat ctggtaaacg ttttccctga cggccggacg cgttcaccgg ccgggcgccg
      181 gaccggtcag cgcttgacgt tgtagtgcag gtgcagcacg cggtcgccct ggatgacgac
      241 gtccgggtct tcgagctgga cggtgccggc gtggctgccg aagtaccgct tgccctcgcc
      301 gaagacgacc ggcgcgacgt ccagcgcgac ctcgtcgacg agcccggccg agaacgcctg
      361 cccggcgacg tcaccggcgg ccacgcagac cagcccgtca ccggcgagct tcttggccag
      421 gttgatgccg ttctcgatcg agtcggccgt gtggaagggc gcgtccggga accgggccag
      481 ccactcgtcc ggcagcggcc ggtgggtgac gacgacgagc tcgtcggcgg acgcgggctt
      541 tccgccccag ccgtcgaacg tgtcgaacag gtgccggccg atcaccgtga ccttgatggc
      601 gtcccagaag ggctgcacgt agtccgcgga cgcctggctg acgttgaacg accactcgtc
      661 gttggcgcgg atcgtgacgt cgccgttcga gtaccagtcg aacagcggcc cgaccgagtc
      721 gtccgggtag gcgatgtagc cgtccagcga gaccacggcg tgtacgacaa ctcgggccat
      781 tgcatccgct ccttgatttc atcggcggcg caggacattc ctgcgcccgt tggcgatgct
      841 atcggcgagg ccgcggcgcg catctcccta attgctctcc tcggcgagtt cgacgatgag
      901 accttcggga ccgcggacgt agcacatccg gaagatgttt tcgtaattga cgatctcgcc
      961 gatggtgtcc agtccttttg cccgaattcg ctcgatcgtg ctgtccagat tctcgacgat
     1021 gtacgcgatg tgccggaacc ccaaccggtt caccgccggt gcctgcgcgt ccgctgaatc
     1081 cgtcgccggg tcgaccgggt ggtggaactt ggtcagctcc agttttccgc tcccgtccgg
     1141 cgcgttgacc atcaccattt ccagccgcac gccctcgagt ccgaggatcc gctcgaccca
     1201 ctcgtcttcg atccgcatcg gcgcggcgac ttcgaagccg aggtcggcga agaaagccgt
     1261 caccgcttcg aggtcgtcga cgatcacacc gacgtgctcg aaccgccgca cgccaccccc
     1321 ggccgcaccg ctcatggtga attcccctcg ctcgtcggac gattcaggtt cgtcgcgaaa
     1381 cctacgcggc cgcctgcgcc cccggccacg gcgaccggcg ccccacgtga cccaggtgcg
     1441 cggtcagcag gtcggcgtcc gccccgactt ccacgggcgg cccgaacatc ccgagcgccc
     1501 gcgcttcggg cccgacgagc acggcagcga acttctgtgc cgcggcgacg acctcggcgt
     1561 cgggcgcgta ggcgatcccg ccgccgctcg ccaggtccca gccgtgggtg agcacctcgt
     1621 tgaggcacat caaggcgtgc accgaaccgg gcgtgtccgc gccgcccttg cccggggtga
     1681 cccggtcgag gatccccggc tcggaaaagg cggtgacgca tcggtccccg agcgcgcgaa
     1741 cggtggccgc ggggtccgcg ccgagccgtt cggtgtgcgc caggtgctcc agccggtccg
     1801 gctgcgagtc cgcggggtcc agcgccggcc cggtcgccgc ctgcgtcgcc aacccgacca
     1861 tgccgagcat gtggttgccg acctggcgga ccgaccaccg ttggcacggg ctcggcccgt
     1921 tccaggccgc cggttcgatg gcggccacca cgtccgccgt cgtccggaaa gcgcgctcga
     1981 gcagtgtggg caggtccatg tccgtcctct cgtcgccggc tttcctccaa tctggaacat
     2041 gatcaagcaa acgatcatct cgctgcgtgc gaggttcgcc tttgcggtgg tcagcggctc
     2101 ggaggcaagt gcttggcgta ccggggaacg ggccgggtgc ccacaagtga cggtcaggca
     2161 gcccggttgg cagcggcctg gtccggcacc ggatcgttcg ccgtccgaag ggtgtctccg
     2221 cacggtgccg cgagcccgcc cgtcgtcgga tccgaactgg acagtgcggt gccgcccgcg
     2281 accggtggtg cggtgccgga atgctccgcg gccgcccgga aggtcaagtg gtgcagcttc
     2341 ctcaggtgcc gctgcaccag accggcccgc cgcgcttcgg cgaagatggc ccgtgcggcg
     2401 ccgttctcgc ggcgacggcg gtggcgcggg gtggatccgt ggtccgccac gatcgtgtcc
     2461 tccagtgggt gtgccggatc ggtccggcgt gtcgggttga cgggtttcat tgctcggtgt
     2521 gaccgttcgg tcgcgcgcga gacacgacgg ccgggttcgc cgcctgggta acaaggttgt
     2581 ctcgactgga actcggcccg gtcaggttgg gccacctggc cgctcgcacg gtgggtcgtc
     2641 cggaccttcg cgcgctgcgg gggaatgcgg gccgccaccg tccactgtgg gagcacaccg
     2701 agcaggagaa gctgttccgg tgtggggccg atcacgtggc cagggcacgt cgtgcgcagc
     2761 cgcgcacgac gtcttccctg gtggcgcccc cggcaggact cgaacctgcg acctagagat
     2821 tagaaggctc ttgctctatc cagctgagct acgagggccc gcgccgacgg ctgcagccga
     2881 tcggtaccgg tcgcccggca ccggcagctt agtcgtcgcc atcctctcga tcgttcgaca
     2941 ccggcgaatc gtttcaggtc tcgttcgacg taattcggga gtccggtggc gacaccgtca
     3001 cggcccgcga tccgacccgg tcgggggagg tgcgcggtcc ggcggtgatc ggaaggcgct
     3061 gtccgccggg tgagctgccg ccggggcccg tgggcgcgcc acgccgcaga ggcgcgccca
     3121 cggggtgggg ccgctgtcgg gggagcggcc ccggtcgggc ccggccgccg ccgcgagccg
     3181 gctccccacc ctgggagcgg cggcgggcac ggacccgggt cgggggtcat tcccagatct
     3241 ggaccgcgcg gacgaggaac ggcgcgttcg gcacgaacgt gccgccaccc gggtaggtga
     3301 tgaaatcgcc ctcggtcgag cattcctcgt cctggtacac cgtgacgtca cggctcatca
     3361 gattcgcgaa cgaggatccc gcgaatcctt cgggcagcgg aatgcattcc ccgggattcg
     3421 cggtgcgcag gtcgaaccgc tgggactcgc cgccgtaggc gtctgccgac cagaggcaga
     3481 attcgccctt ctgacaggcg ggatccggcg gcggcgaagc ggcctgggcc actccggtgc
     3541 tggtcagcaa ccccagcacg gccaggacca gcaactgcgg cagccgggaa cggagacggc
     3601 tcgaagacat gtggattccc ctctggagtt cgcggcccgg tggccgcggt gaacgtgtga
     3661 ttccagattg gcgcgccggg gaagggttgt cagcccgcga tttccattct gtggacaact
     3721 cggggaaccg gccgcggttg tccacaaggc cgcgaacggg gcttgcgaac gggccgttcc
     3781 ggtgttacgc gcgccgtgtc aagctgaggc cgcggaaggg ggagccatgc gggatcgggt
     3841 cgcggtgttc accgtgttcg ccctcaacgg cctggcgatc ggctcgtggg cgacgcgcac
     3901 gcccgcgctg gccgcccagg tgcacgcctc gcccggcgtg ttcgggctcg cgctgctcgg
     3961 cgccagcgtc ggcctgctct cggccgcctc ggtggccgga agggtcgtcg aacgcctcgg
     4021 ggccagggcg gtcgtggccg ggaccacggt cctggcggcc gtcgccctgg tgctgatcgg
     4081 ctacgcgccg ggcgtcccgc tgctggccgg cgcgctgttc gtggtcgggg cgagcgtcgg
     4141 gatgctcgac gtcgcgatga acgtcgccgc ggtggccgtc gaacgccggg cccggcggcc
     4201 ggtcatgccg gtcttccacg ccgggttcag cgtgggcgcg ctcgccggtt cgctggccgc
     4261 ggggctcgcc gcgggacacg gctggtcacc ggcgcgccac ctgaccaccg cggccgtcgt
     4321 cgcggtcgtg gtgctgctgg tggtggtccg cgcggtcccg ggcagccgtc cggagcacgc
     4381 cgccgcggtc accggccgcc gggcgccgat ccgccgtccg gtgctgtggc tgctcgccgc
     4441 ggtggcgctg tgctcggcgg tcgccgaggg cgcggcggcg gactggtcgg cgctgctgat
     4501 gaccgccgaa cgcggcgtgg gccagggggc ggcggcgctg gcgttcgccg ggttccagct
     4561 ggtcatggca ctggcacggc tggcgggacc gtgggcgcaa cggcggttcg ggccgacccg
     4621 ctgcctggtc gcgggcgcgg cgctggcgac ggccggcttc ctcgcgacgg ccacggtgcc
     4681 ggccgctgtc gtcgcctacg ccggcttcgc gctggccggt gcggggctcg cggcttcgtt
     4741 ccccctcgcg ctgagcctcg ccggtgacgc ggggaaacgc ggtgacgggg ccggtgggga
     4801 gcgcgagatc gggttcgtgt cggccgtcgc ctacgccggg ttcctcgccg ggccgccgat
     4861 catcgggggg atcgcgcagg cggtcgggtt cggggccgcg ttcgggttcg tcgcgctggt
     4921 cgcggcgctg atcctgcccg cggcggtggc ggcccggcgg tcgcggcgcc gggaggcagc
     4981 cgggcagccc gcgccggagg tcacccgtgg ggcatcggat cccggttgag ctgctcctcg
     5041 tccagcggct ccgggtgcca ccccagccgc acccccaggt cctggacgcg ccgcaggccc
     5101 agtggcgggt aggcggccgg cgccgttccc accgtgcccg gcaccaggac gcgcaccacc
     5161 cgcacgccgt ccgccgccgc ttccggcgtt gtcaggtcga cgtacaggac ctcgtgcccc
     5221 gccgccgcga cgcacgccag cagggcgtcg agcgagtcgt ccgccggccg tgggatcgcc
     5281 gcccagtcga ggggcggccc gcccgtcgcc caggccagtg ccgccgcgcc ggctcgcggg
     5341 tcgaggtgga actgctggtg gcacagcagc tccacgaggt ccgccgggcc gtagtggtcg
     5401 gcgtagtccc gctccgcccg ccacgggcgc aacgggcccg ggcggccgcc gtagcccgcg
     5461 atgatcgccg agagcgcgtc gcggtcgtcg agcgtgcggc acgtgatctg cagtgtgtac
     5521 gcctccgcca gcgccttcag ggccgcctgc tcggggtcgg gccgcgtcgc cgtgccgacc
     5581 gtcagccagc cctcgggcga ccggaccgcc gcggcgagca ccggcacgcc gaacgcgttc
     5641 gcgatcgacc agaaccggac ctcgtcggca ccggccagtg aggccaggcg cggggtcggg
     5701 gccagggtgg gctggggcgc gccgttgagc caccagaccg ccgaggcgtc gtgttcgacg
     5761 atttcctgca agccggaccg gatcgcggct tcgagggtct cgcccgccgc cgtgccgccg
     5821 atcgccggga acgcgtggag gggctcctcg gacgccttcg ggacgtgggg ccagccggtg
     5881 tggaccagga aggcgggcac gtggacgtcg ccgccgccga gcgaggtggc gcggacccag
     5941 tgcacgggcg aagaggggag gaacggcgtg aacgggaacc cgggggtggc gtactggcgc
     6001 ggcgaataca gcgcgagccg gccgggggac agcgcggtca gcccgcgcgc gaccaggtcg
     6061 tcgtggctgc cgtggacgac gtcgcggggc ggccgggtcg cgcagtaccg ctccaccgcc
     6121 tcgccccggg cggacacgcg tgcgtgcgcc gggtcgtccc acgatgtgcc gaagccctgc
     6181 aggtcgccct gccacggcag ttcggcccgg acgtcgcgga tgtcggccac cgcgaggtgc
     6241 agggccgccg gagtgcctgc caggccggcg aaggaccgca accgggtgac gatcgggccg
     6301 gagcccggcg cggaacggcg ttcaccgctc cggccgggca gcccttcgtg cagagcggtc
     6361 atcagcgctc cgggaggtac gccgcgacgc acgtgttgac cgtgaggctc gccgccacgc
     6421 acgtgttcac gacgaggctc gccgcgacgc aggtgttgac gaccagggct tccggggcgg
     6481 tctcgacgga gaactgctcc tgcttcatgg cgctaccctt tccatcgacg gcccaccacc
     6541 ggttccggtg gcgggatccg gggagtgctc cccgttccgc gcgtccgagg cggccacctc
     6601 gcgcggaagc ttggtaccga ccgcgaaccc cgcggcggcc agcgcgccga gcgcggcgca
     6661 ggccagccac acggccgcgg ggccaccgcg gtccagcagc cagccgccgc cggccggccc
     6721 gagcagggcc gcggccgacc acgcgccggt gtagagggcc gcgtaccggc cgcgctgcgt
     6781 gctgggtgcc cggtcggccg ccacggagct gccgacgggc atctgcagca tttccccgac
     6841 cgtccagacc agggcgatga cggcgtagcc gagcagcccc tcggcgaaga agttgagccc
     6901 gaagccccag ccgatcagca gcgcgctcgc caccagcagc ggtcgtcgcc ggtgccggcg
     6961 cgcccaccgg gtcagcggga gctgcaggaa cgcgatgagc agcccgttca aggcctgtac
     7021 gaggccgaac gcgcccgcgg tgagcccggc gccgaccatg acgaccggca gcgtcgtcgc
     7081 gctctgttcg tagacggagc cgatcgcgag ggtggcgagc aggaagacgg cgaaccgcag
     7141 gtcgaaccgc aagggcggcc gggcgacgcg ggcccgtggg gtgaacggcg gccgggtttc
     7201 gggcaccttc agcagcacga cgagcgcgca caacgcggtc gcggcggcgt cggcgatgaa
     7261 cacgacgggg tagccgaagc ccaccatcac gccggtgagc gcggcggaga cggcgacgcc
     7321 gacgttgacc gcccagtagt tgagcgagaa ggcgcgcggc cggtcggcgg gcggcacgac
     7381 gtcggcgagc gcggcgttca tcgccggccg ggccgccacg gaaccgacgc cgacggcgaa
     7441 ggccgccgct tcgagctgcc agcgcccgct cgccgcgccg agcgcgacca tcgccaccgc
     7501 ggtgacgacg tgcgccagca cgagggtcgg ccgccggccc agccggtcgg cgaggacgcc
     7561 gccggccacg gtgccgagca cgccacccag cccgatcagg gcgatgacga acccggcgta
     7621 ggacaccggg tacccgtgcc cggccgtcag cgacagcgcg agcacgggcc gggtgaaggc
     7681 gccggcccag ttgaccagga ggctgatcca gatccaccag aacaccggcg ggagggtccg
     7741 ggggctccga cgggacgtca tcgtgtgcgt cacccgtcgg cccccttccc cgttccactg
     7801 gtttctcgtc ccgagccggg tgccgtggcc gtctggatca cagcgacgcg ggcgttcccg
     7861 gggctcctcg caggagagcc agttgtgcgg ttctcccatc gagccgggtg ccttccgctg
     7921 ggtctcagct gggaggcgca cccccggact catgggtaaa tcatggctcc cggcatggag
     7981 atcgctcgtc tcctcgattg cccgccgtct gggccgaacc cgttgacagt gccgaaccgg
     8041 tgggggcaag ctgaccctgt cggtaaactg agatgaatgc ggcgacgctt ccatttcagg
     8101 tcggctgatc agggtagggg ccttgatccg gggaggacgt cgtgagggtt gtgctgctcg
     8161 gcggagcggg ggcgctcgcc ggcgggagcg gtcggtgggg tgcgctttcg ctggtgcagg
     8221 gcgccgtacc gaggctcgtg ctggtgccgg tcgtgttccg gctggtgctg ttcctccagg
     8281 cgctgtgggc cgcgggcggg cgcacggcgc cggtgctctg gctcgccgtg gcgttcttcc
     8341 tcgtgccgaa cctcctcgag gtcgcctggg tcttccgccg cgccaccggc atccgcccgg
     8401 tgctggtcgc cgacaccgcg tacacgctcg tcaccagcct ggccgcggcg gcgttcgcgc
     8461 ccggcctgct ggggctgacc tggccgaaca tcatgggcac ggtcatggtc tggaccctgc
     8521 tgcgcggcgc gcccgccggc gcggcggcca tcgccggagc cgtgctcctg cgctacggca
     8581 cggccgcggt ctcgggcacg tccgcgccgg cgacgtggat cctgccggcc ctgctcgcgg
     8641 ccgcggcgac ggcgctggcg atcgtgctgc tggtcgccgg ctcgatgcgg ttcgcgcacc
     8701 ggctgggcga gcagcgcggc cgagccgccg aacgcgagcg gcaccgccgg gacgtccacg
     8761 acaccgtgct gcaggtgatg gagtccctcg ccctgcccgc ccccgacgac gcgctcgacc
     8821 ccgtcggcag gctcgaccag gtccggcgca ccgcccgggc gcacgcgctg cgcctgcggc
     8881 tgagcctcga cggcgacgcg cccgccgagc cgggtgggct cgaacaccgc ctcggcgcgt
     8941 tggcggtcga gatgacagcg gacggcctgc gcgtcgacct cgtcgtgctc gcgaagcctt
     9001 cggacgtacc cgaagccgtc gtcaacgcat tgcacgacgc ggcccgcgaa gcgctgcgaa
     9061 acacccttaa gcacgcgaag accgccagag cggtggtctg cctcgaagag cgcgaaggcg
     9121 tcatcacggt gtccgtgcgc gaccacggga ccggcttcga ccccggcgcc cgccgcgccg
     9181 ggttcggcat cgacaactcc atccacgcgc ggctggccga ggtcggcggt gccgcgcgca
     9241 tcgattcggc gccggggcag ggcacccgcg tcgtgctgac ggccccgcta gagctgcgtt
     9301 tccccgtcgg gtgagtcccg ccactgggcg aacgggcggt ccagtgtcca cttgccgtcc
     9361 tgctcctcga ggacgcggta ctcgcaggtc cccgggttgg acagcgactc gaacagctcg
     9421 atgctccagc cgaagaggcg gcggcacagc aaccgcagcg tgagtccgtg ggagacgatc
     9481 aggaccgtct ccgggtgacg ttcgtcgcgc aggcgcaggt ccgaaaggaa cgccgcgacg
     9541 cggtcgtcga cgtccgcgcc cgactcgccg aacgggagcc ggtagaagaa gtgcccgaac
     9601 tcgttgcgcc gcgccttctg gacctcctgg tcggccgggt cctgcaggtt gccccagtcc
     9661 tgctcgcgca gccgcggctc ctgcagcagc cgatcgcacg acgcctcgat gtccagcagc
     9721 cgcaacgttt cccgcgtccg caggtacggg ctcacgtaga cggcgggccg ctcgccgtcg
     9781 agcagccggg cgatctccgg gccggccgcg agcgcttccc gctcgccctt ctccgtcagc
     9841 ggcagcgcgt ggtcggggat ccgcgtgtag gcgagctcgt cgacgttgcc gagcgactcg
     9901 gcgtgccgca gcaggatcat cttcacggct cccatcctgc ccgtcacacg ggcctgaccg
     9961 gggctgggca taccctcagg ggcgtgtccg agcagtccgt cacgaaatcc gacgtcccca
    10021 cgcagcggcg ggccagcgtg ctgcccctgc tctcggtcgg cgtgctgctg gccgccgtcg
    10081 tcgcggtcgg gctgatcgcc ctcaccggcg gggcgggcta cgtcatcgcc ggcctccccg
    10141 atccgggcct ggtcacgcag tacggcgtca ccgtcgtgcg cgtgctgtcc gaagccgcgt
    10201 cggtgatctg cgtcgggtcg ctgttgctgg ccgcgttcct ggtgccgccg cagaagtcgg
    10261 gcacgctcgg ccccgagggc tacggcgcac tgcgcgccgc cgggatcgcc gcctgggtgt
    10321 ggttcgccgc cgccctgctg tcggtcgcct tcaccgccgc ggacaccgcc gggaagccgt
    10381 tcggcgacat cctcgacccg cagacgctgc tcgacctcgt cggcgccatc gagcagccga
    10441 aggcctggct gtggacggcg ctgatcgcgg tgctcgtcgc gctcggctgc cggctcgcgc
    10501 tcacctgggg ctggacggcg gtgctgttcg ggttcgccgt cgtcggcctg gtcccggtcg
    10561 cggtcaccgg ccactccgcc agcggcggct cgcacgacgt cgccaccaac agcctgctgt
    10621 tccacctggt cgccgcggcg ctgtgggtcg gcgggctgat cgcggtgctg gcgctgggtt
    10681 accggcgcgg gagccacctg agcctggccg cgcagcggtt ctcgcggctg gcgctggtgt
    10741 gctgggtcgt gatgctgctc tccggcgtgg tcaacgcgct ggtccggatc ggcctgaacg
    10801 acctcttcac caccgactac ggcctgctgg tcgtggccaa gacggtcgcg ctgctgctgc
    10861 tcggggtgtt cggccaccag cagcgccaga agggcgtcgc cgacctcgtg gacggcaagg
    10921 gcggcgggca gctgctgcgg ctggccgcgg tcgagatcgt gatcatgttc gtcacgatcg
    10981 ggatcgcgtc ggggctcgcg cggacgccgc caccggcgga cgcggtcacc cagccgtcca
    11041 ccaccgaact gctgatcggc tacaacctcg acggcccgcc gacgctgtgg cggctgctct
    11101 tcgacacccg cttcgacctc gtctacggcg tcctcgcgct cgtcatgggc gggctgtacc
    11161 tggcgggggt ccggcggttg ctgcgccgcg gcgacccgtg gccggtcggc cgcacggtcg
    11221 cctggatcag cggctgcgtg gtactgctga tcgcgacgtc gtccggcatc ggccggtacg
    11281 cgcccgcgat gttcagcgtc cacatgggca accacatgct gctgtcgatg gtggcgccgg
    11341 tgctgttcgt gctcggcggg cc
//
