LOCUS       NZ_JALGRP010000022     10891 bp    DNA     linear   CON 13-SEP-2024
DEFINITION  Microbacterium sp. VKM Ac-2923 n22, whole genome shotgun sequence.
ACCESSION   NZ_JALGRP010000022
VERSION     NZ_JALGRP010000022.1
KEYWORDS    WGS; RefSeq.
SOURCE      Microbacterium sp. VKM Ac-2923
  ORGANISM  Microbacterium sp. VKM Ac-2923
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 19448)
  AUTHORS   Tarlachkov,S.V., Ospennikov,Y.V., Demidov,A.N., Dorofeeva,L.V.,
            Starodumova,I.P., Prisyazhnaya,N.V., Nadler,S.A., Subbotin,S.A. and
            Evtushenko,L.I.
  TITLE     Draft Genomes of 9 actinobacteria of the family Microbacteriaceae
            associated with insect- and nematode-damaged plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19448)
  AUTHORS   Tarlachkov,S.V., Ospennikov,Y.V., Demidov,A.N., Dorofeeva,L.V.,
            Starodumova,I.P., Prisyazhnaya,N.V., Nadler,S.A., Subbotin,S.A. and
            Evtushenko,L.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2022) All-Russian Collection of Microorganisms,
            G.K. Skryabin Institute of Biochemistry and Physiology of
            Microorganisms, PSCBR RAS, pr. Nauki, 5, Pushchino, Moscow Region
            142290, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-MAR-2022
            Assembly Method                   :: SPAdes v. 3.15.4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 536x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022815605.1-RS_2024_09_13
            Annotation Date                   :: 09/13/2024 06:06:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,615
            CDSs (total)                      :: 3,562
            Genes (coding)                    :: 3,546
            CDSs (with protein)               :: 3,546
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:09:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 10891
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALGRP010000022.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10891
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10891
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10891
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[3677:5891](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(3678..5891)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    2..16
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.13"
                     /tool="antismash"
     gene            complement(1143..1559)
                     /locus_tag="MT347_RS17780"
                     /old_locus_tag="MT347_17775"
     CDS             complement(1143..1559)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173573.1"
                     /locus_tag="MT347_RS17780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MT347_17775"
                     /product="amino acid transporter"
                     /protein_id="WP_243502677.1"
                     /transl_table=11
                     /translation="MTDKPTTRKDLMKPVQLLGLAFVAALFAGFVTLMSMGFFQSRPAD
                     QIQNALIVALVAAGATFIVVLVSVALMLLAVDPAKVTATVDRPLLLPTDAPDAATPGAS
                     TTDPAAGPAAAAASEQPVRPSAETPDDDAATPKA"
     gene            complement(1556..2188)
                     /locus_tag="MT347_RS17785"
                     /old_locus_tag="MT347_17780"
     CDS             complement(1556..2188)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584738.1"
                     /locus_tag="MT347_RS17785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MT347_17780"
                     /product="acyl-CoA synthetase"
                     /protein_id="WP_243502678.1"
                     /transl_table=11
                     /translation="MTTSSPARAFDIRHLQLSRAAFAALAAVMVTFSSDHSAALGLGVF
                     SGFALATGLVFGLSAWLVFPRGERMIPVLLASVSILAGMVSSVAGWRTTGVFFGVVIAW
                     AFVSGLIELLGALRDRKAGRTAHTRDGVLIGILGLVLAVVLLLTPMQYALDYDITGAGS
                     FTLTGITIAVGLFGGYAAVIAVFLAIAGFSPRRPEPVAAVSPEEAAS"
     gene            complement(2492..3685)
                     /locus_tag="MT347_RS17790"
                     /old_locus_tag="MT347_17785"
     CDS             complement(2492..3685)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MT347_RS17790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MT347_17785"
                     /product="hypothetical protein"
                     /protein_id="WP_243502679.1"
                     /transl_table=11
                     /translation="MRELVARPVRLRHPAPFPSAFQHLQVALPAAAGVDAHAVVVDLAE
                     GIDDELVDAAVRRHWWSADHGQLVPRRATARELVAEGGPVLRVRALGWVDPAERALPGF
                     PLADDDDDAPRLWVRARRDDASGAAEGWMPYTLAIARPADARPDEPLAHPPLLGGFGAG
                     VDPVAASDAAWRSIVIDDALWCWWTGLARAASVDPSPEVRRLWADADLGLTLRRLDRGV
                     LAPVTLAVVDDGVALTIGGGVGLDAAAQRAAIAQALWQLVIARALDDPAVSLSAPGVVG
                     HRPDRRYAPEGVVDRRRLLDPLAHVQLSLDPGVRADVRRRLDAVDDDAPTVAAEPVETW
                     TVALAPDAGVVRMFSPAAVPVPWGAFRLDPALVARAAARTGRAPTPAAERQTPYPGW"
     gene            complement(3678..5891)
                     /locus_tag="MT347_RS17795"
                     /old_locus_tag="MT347_17790"
     CDS             complement(3678..5891)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03604"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03604.1"
                     /locus_tag="MT347_RS17795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MT347_17790"
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="WP_243502680.1"
                     /sec_met_domain="YcaO (E-value: 1.3e-61, bitscore: 199.1,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03604 (E-value: 4.5e-77, bitscore:
                     249.4, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPPIPLVTDARPARTGVEVRPLDERRSLVTVAEQHHIVDVPAAVV
                     QVLLWAWHEGRDDLIGAEFAAAAEGLRPLLAAAGPAVAPVASVPSSRPAAGPAVRAVLA
                     GDDDVVAAARARLRGDDDVQSAPVAAPRTVATAAFSTSRLLVVALRGGREREFVDLDRI
                     CHDLRVPWLAVELSRGRLWVGPLVTPGVGASYEDAAARRTASARNAEVHRALRTPAVGG
                     DQGPDAADLPALLDAMWELAATAADAVARGGLDAAPGDVVHELWLDDGVVRRAAHPVLP
                     LPHRRTRHRAHDVDDLVDERTGVITRIRDVRHHERVPAGLVTRQADVADIRAVTSWANN
                     VLCQGSAFDDAASAHAAAVGESVERYCGNILDTLPVRHGSFAQLRRSGVPALDPRRLVL
                     YSERQYAAPGFPFVPLDSDLPVHWVPGRSALTGGEVWVPASMVYVNWYSADVAAAPPTN
                     FCAFAGIAAGPSEDFAVTSAIEEVVERHATMVWWLNAQPLPRVEGVPAPPLVAGMRASF
                     VHLDNEFGVPVAAAVLHDDDARLVNVGFSARRDFGSAASKALTEAYTLQEGSRDLLHAD
                     GLHWRVMTEGELNGRAFKPWRADRRYLDDFRPDMHDCDDLMVQQQVYLDPRAGDRMRPL
                     LEPTATRPLDALPRLAERSRATMVAALERADVEPIVVDITTPDVAAAGLSVVRVIAPGT
                     IGNAPAAFPFLGHGRVQRVAVDLGWRAEALDESQINLFPLPHA"
     gene            complement(6303..8144)
                     /locus_tag="MT347_RS17800"
                     /old_locus_tag="MT347_17795"
     CDS             complement(6303..8144)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012869.5"
                     /locus_tag="MT347_RS17800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MT347_17795"
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="WP_243502681.1"
                     /transl_table=11
                     /translation="MSALRPLGMLLPHLRRRRGLFLGVVAVVIVGMLAAVAVSVLSVSA
                     ASSLVTAGDPARAAAIALVAAVVASVVVAGLAAWSEQWFAHVLAYRVIDALRLDVHRTI
                     ARLAPVGLARRRTGDTVAAAMGDVEALEWFAAHTVAQVTAGVLACAVIDTTAAVLFGPV
                     WLLLVPAQVALVLIPLSFVRRAGRQGEELRAALARVSARVLAARSAARDVVLLGRVDAE
                     ARAVADETGAVQRARRGLALRAGVEQACTDAMTVAVLLTTLLLVGSAVAGGADAAIAPT
                     LVVLAAASLGPAAIAAGALARVGETAQAARRVDTVLRAPDVRPVDPVMREGAHDPGPVD
                     PSPHDGDPTPLGAGADPRAGARHPGSIDARALTAAYPGVGSAALVDADVRVAPGEHIAV
                     VGPSGAGKTTLALVLTRLLASRGTLRIDDVDTAAEAGADTRSRVVLVPQQPHVFRASVR
                     DNLLAPDADDPTLWDALERARLAAHVRRLPDGLDTLLAERGATWSGGERQRLGLARALL
                     REPSVLVLDEPTASLDTATEAEFLAALSLARAGRTTIAITHRPTLMRAMDRVLFVDGGR
                     VADTGPHDELRARSPRYRAVVDGASQSPSAHSDPERR"
     gene            complement(8141..9979)
                     /locus_tag="MT347_RS17805"
                     /old_locus_tag="MT347_17800"
     CDS             complement(8141..9979)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012869.5"
                     /locus_tag="MT347_RS17805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MT347_17800"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_243502682.1"
                     /transl_table=11
                     /translation="MVHLRLLRLWRGHRSALVLLVAIGVAISLTWATQALLLSQLFAAL
                     LRGSALVDAALLPVLGALAAVLLVRPALALVRAAVAHRAMDAVKRDLRARSFAALIERS
                     ATRPGHGRSGGDQATVVDGVENLDAYLSSYVPQLAVTGIVFAGVGTALVLVDPLIGVVA
                     VVAAAVLPLAPRLWDRVLAKRGGDHWEAYQELHAEFVDSMHGMTTLVAFGQERRREREL
                     AAASDTLLRRTLRQLSVSLIESGLSQFALLAVPALVLVLVAVRGGDLGAFAVFAAVLLS
                     IELVRPLRELSAAWHAGYLGTFSGPPVLDLVAADRGRRDAPAVDRAAVALPLVAEGLRA
                     RHPGAKTDAVTEATFRIDAGLTAIVGPTGAGKSSIAAVLAGVLPAEAGEITFGGERILG
                     RVSGVGDSGAPGSPARDARMDAAAAAEQLRARVALVAQDPALFTGDVRSEILLGVGVGA
                     GASRAVDVSAIAARAGIGTGETALDLDTALGDGGGVLSGGQRQRVAIARGDAQQRRVLV
                     LDEATSALDPASEARLVSALRAAHPDDAIVAVTHRLAVAEAADRVIVVVDGRVVETDHP
                     RVLRARGGAYARLLAAEGDPTAEAEAVVPAAHGAPA"
     misc_feature    9993..10008
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 19.77"
                     /tool="antismash"
     misc_feature    9993..10011
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 25.11"
                     /tool="antismash"
     misc_feature    complement(9995..10008)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.78"
                     /tool="antismash"
     misc_feature    complement(9995..10009)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.52"
                     /tool="antismash"
     misc_feature    9996..10009
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 16.44"
                     /tool="antismash"
     misc_feature    complement(9996..10011)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 25.68"
                     /tool="antismash"
     misc_feature    9999..10012
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.1"
                     /tool="antismash"
     misc_feature    9999..10014
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.79"
                     /tool="antismash"
     misc_feature    complement(10237..10251)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
ORIGIN
        1 cttcacactc cgcaaaacgc tgaacacagc cacgcccctc aatcagggtg ttgcactgac
       61 ccaatgagac cgccctctcg cagactcctc cgctcacccg atgccctcca ctcgtcaccg
      121 ccgcgccgcg gctggaaacc gcgcacgggt ggatgaggcg tagggcggtg gcggtccggt
      181 gcgaggccgg gccggggcgg tgccggtccg gtccggtgcg gtccggtgcg gagcggagga
      241 gatacggcga cgagaggatc agctccgctc gcagactcct ccactcacgc gatgccctcc
      301 actcgtcacc gtgccgcgcc gcagccgaaa accgcggccg ggcggtccgg tgcggtgcgg
      361 aaccgggccg gtgcggtccg gtgcggtgcg gaaccgggcc ggtgcggtgc ggtgcggtcc
      421 ggtgcggagc ggaggagata cggcgacggg aggatcagcc ccgcccgcag actcctccgc
      481 tcacccgata ccctccactc gtcaccgcgc cgcgccgcag ccgaaaaccg cggccgggcg
      541 gtccggtgcg gtgcggaacc gggccggtgc ggtgcggtgc ggtccggtgc ggagcggagg
      601 agatacggcg acgggaggat cagctccgcc cgcagactcc tccgctcacc cgatgccctc
      661 cactcgccac cgcaccgtgg ctgaaaaccg cgcacagcct ccactcgcca ccgcgccgcg
      721 gctgaaaacc gcgcacaggt ggatgcagcg tagggcggtg gccgtccggt accgggccgg
      781 tgcggtccgg ggcggtgcgg aggagatacg gcgacgggag gatcagctcc gctcgcagac
      841 tcctccgctc acccgatgcc ctccactcgc caccgcgccg cgccgcgccg cggctgaacc
      901 gcgcacgggc ggatgcaggc gcagcgcgca acgcgcgcgc gtggccggcg gcggaccgca
      961 gcgaagggcg agcgcaacca ggcgcagcac ggggccgcgc gcgggcacgc gtgccgtgag
     1021 aaccggtggg gagcggagga gatgcggcga ccggaggatc gattcccccc gcgtagtcct
     1081 ccgtacaccc cacgccctcc gcccgtcacc gcaggagggc aggcgcaacc gggcagcgcg
     1141 cgtcaggcct tcggggtggc cgcgtcgtcg tcgggcgtct cggcagacgg ccgcacgggc
     1201 tgctccgacg ccgcggcggc cgcaggccct gcagcggggt cggtcgtgga ggcgccgggc
     1261 gtcgcggcat ccggagcgtc cgtcggcagg agcagcggac gatcgacggt cgcggtgacc
     1321 ttggccgggt cgacggcgag cagcatgagc gcgacgctca ccaggacgac gatgaacgtg
     1381 gcaccggcgg cgacgagcgc gacgatgaga gcgttctgga tctggtcggc ggggcgcgac
     1441 tggaagaacc ccatcgacat gagcgtgacg aaaccggcga acagggcggc gacgaaagcc
     1501 agaccgagca gctggacggg cttcatcagg tctttgcgcg tggtgggctt gtcggtcatg
     1561 acgcggcctc ctcaggggat acggccgcga cgggctcggg gcgacgcggc gagaagccgg
     1621 cgatcgcgag gaacacggcg atgaccgccg cgtagccgcc gaagagtccc acggcgatgg
     1681 tgatgccggt cagcgtgaac gagcccgccc cggtgatgtc gtagtcgagc gcgtactgca
     1741 tcggtgtgag cagcagcacg accgccagca cgagacccag gatgccgatg agcacgccgt
     1801 ctcgcgtatg ggcggtgcgg ccggccttgc ggtcgcgcag cgcgcccagc agttcgatga
     1861 ggcccgagac gaaagcccag gcgatgacga cgccgaagaa cacgcccgtc gtgcgccacc
     1921 cggcgacgct ggacaccatc ccggcgagga tcgagacgga tgccagcagg accgggatca
     1981 tgcgctcccc gcgcgggaac accagccacg ccgagaggcc gaagaccagc ccggtggcga
     2041 gcgcgaaacc gctgaagacg ccgaggccga gggcggcgga gtgatccgag gagaacgtca
     2101 ccatgacggc ggcgagcgcg gcgaaagcgg cgcgcgaaag ctgaaggtgg cgaatgtcga
     2161 aggcacgcgc aggggaggaa gtggtcacgg agtgtcccgg aaaggcggag tggatggcat
     2221 ccagtctatt cctcggcacc tgggcggccc ccgcggaacc tcgacggcga aggagcgagc
     2281 tcgtgagggg cgccaccgac ccgcggcggc tccggcgcgc gggcgccggg cgcggacggc
     2341 gagtacggcg gtgagcatca ccacggcgag caccaccacg gcgagcgccg cggccttcag
     2401 cgcgacctgg acgcccgtgg cgaagggtgc ggcggagccg atctcctcgg catccggcgc
     2461 gtcggtgacg tcctgtggga tgcggcggag ctcaccaccc ggggtaggga gtctgccgct
     2521 cggcggcggg ggtcggcgcg cgaccggtac gcgccgcggc ccgggcgacc agtgcggggt
     2581 cgagccggaa ggcgccccag ggaacgggga ccgccgcggg cgagaacatc cgcacgacac
     2641 cggcgtcggg ggcgagggcg accgtccacg tctcgacggg ctccgccgcc acggtgggcg
     2701 catcgtcgtc gaccgcgtcg agacgccgcc gcacgtcggc tcgcaccccc gggtcgaggc
     2761 tcagctgcac gtgggcgagc gggtcgagga ggcggcggcg atcgacgacc ccctcggggg
     2821 cgtagcgccg gtcgggacga tgccccacga ccccgggcgc cgacagcgac accgccggat
     2881 cgtcgagcgc gcgggcgatc acgagctgcc agagcgcctg ggcgatcgcg gcacgctggg
     2941 ccgcggcatc cagaccgaca ccgcccccga tcgtcagcgc gacaccgtcg tcgacgacgg
     3001 cgagcgtgac cggggccaga acgccgcggt cgaggcggcg cagggtcaga ccgaggtcgg
     3061 cgtcggccca cagtcgccgc acctcgggcg acgggtcgac ggatgccgcg cgcgcgaggc
     3121 ccgtccacca gcaccacagc gcgtcgtcga tcacgatcga gcgccaggcc gcgtcggacg
     3181 ccgcgaccgg gtcgactccg gcgccgaagc cacccaggag cgggggatgc gcgagcggtt
     3241 cgtcgggacg cgcatcggcg gggcgggcga tggcgagggt gtacggcatc catccctcgg
     3301 ccgctcccga tgcgtcgtcg cgccgagcgc gcacccagag gcgcggggcg tcgtcatcat
     3361 cgtcggcgag ggggaagccg gggagggcgc gctccgcggg gtcgacccag ccgagcgctc
     3421 ggacccgcag caccgggccg ccctcggcga cgagctcccg cgccgtcgcc cgacgcggga
     3481 cgagctggcc gtgatcggcg gaccaccagt gccggcgcac cgcggcgtcg acgagctcat
     3541 cgtcgatccc ctcggcgagg tcgacgacca ccgcgtgcgc gtcgacgccc gccgccgcgg
     3601 gcagggcgac ctgcagatgc tggaacgccg atgggaaggg agccggatgc cgcaaccgca
     3661 cggggcgggc gacgagctca cgcatgcggc agggggaaga ggttgatctg cgactcgtcg
     3721 agcgcctccg cgcgccagcc gaggtcgacg gcgacgcgct gcacccgccc gtgcccgagg
     3781 aacgggaacg ccgcgggggc gttgccgatc gtgcccggcg cgatcacgcg gacgacgctc
     3841 aaccccgcgg cggcgacgtc gggggtggtg atgtcgacga cgatcggctc gacgtcggcc
     3901 cgttcgagcg cggcgaccat cgtggcgcgc gagcgctcgg ccagccgcgg aagtgcgtcg
     3961 agcggacggg tcgccgtcgg ctcgagcagg gggcgcatgc gatcaccggc gcgggggtcg
     4021 aggtacacct gctgctgcac catcaggtcg tcgcagtcgt gcatgtcggg gcggaagtcg
     4081 tcgagatagc gacgatcggc ccgccacggc ttgaaggccc ggccgttgag ttcaccctcg
     4141 gtcatcacgc gccagtgcag cccgtcggcg tgcagcaggt cgcgcgagcc ctcctgcagc
     4201 gtgtacgcct cggtcagggc cttggatgcc gcggagccga agtcgcgacg cgccgagaag
     4261 cccacgttca ccaggcgcgc atcgtcgtcg tgcagcacgg cggccgccac cgggacgccg
     4321 aactcgttgt cgaggtgcac gaaggatgca cgcatgcccg cgacgagcgg aggagcgggc
     4381 accccctcga cgcgcggcag cggctgggcg ttcagccacc acaccatggt cgcgtggcgc
     4441 tcgacgacct cttcgatcgc gctggtgacc gcgaagtcct cgctcgggcc ggccgcgatg
     4501 ccggcgaagg cgcagaagtt ggtcgggggc gcggcggcca cgtcggccga gtaccagttg
     4561 acgtagacca tcgatgccgg cacccacacc tcgccgcccg tcagcgcgga acgccccggc
     4621 acccagtgca cgggcaggtc cgagtcgaga gggacgaagg ggaagccggg ggccgcgtac
     4681 tgccgctcgc tgtagagcac gagccgacgc ggatccaggg cgggaacccc cgagcggcgc
     4741 agctgggcga acgacccgtg ccgcaccggc agggtgtcga ggatgttgcc gcagtagcgc
     4801 tccaccgact cgcccacggc ggcggcgtgc gccgaggcgg catcgtcgaa cgccgagccc
     4861 tggcacagca cgttgttggc ccacgaggtc accgcgcgga tgtcggcgac gtcggcctgt
     4921 cgcgtgacca ggccggcggg gacgcgctca tgatggcgca cgtcgcggat gcgcgtgatc
     4981 accccggtgc gctcgtcgac gagatcgtcg acgtcgtgcg cgcgatggcg cgttcgccgg
     5041 tgcggcagcg gcagcacggg gtgcgcggcg cggcgcacga cgccgtcgtc gagccacagc
     5101 tcgtgcacca cgtcgccggg ggccgcgtcg agcccgcccc gcgccacggc gtcggcggcg
     5161 gtcgcggcca gctcccacat cgcatcgagc agggcgggga ggtcggcggc atccggtccc
     5221 tggtcgccgc ccacggccgg agtccgaagc gcgcggtgca cttcggcgtt gcgggcggat
     5281 gccgtgcggc gcgccgccgc gtcttcgtac gacgctccga ccccgggcgt gaccagcggg
     5341 ccgacccaca gccgcccgcg cgacagctcg acggcgagcc agggcacgcg caggtcgtgg
     5401 cagatgcggt cgaggtcgac gaactcgcgc tcacggcccc cgcgcagggc gacgaccagc
     5461 aggcgggagg tcgagaaagc cgcggtcgcg acggtgcggg gagccgcgac cggggcggac
     5521 tgcacgtcgt cgtcgccgcg caggcgcgcg cgggcggcgg ccacgacgtc gtcgtcgccc
     5581 gccagcacgg cgcggacggc cgggccggcg gcggggcgcg acgagggcac ggatgccacg
     5641 ggcgcgaccg cggggccggc ggcggcgagc aggggtcgga gtccctcggc ggcggccgcg
     5701 aactcggccc cgatgaggtc gtcgcggccc tcgtgccacg cccagagcag cacctgcacg
     5761 accgcggcgg gcacgtcgac gatgtggtgc tgctcggcga cggtgacgag gctccgtcgc
     5821 tcgtcgagag ggcgcacctc gacgccggtg cgcgcggggc gggcgtcggt gacgagcgga
     5881 atggggggca tgggcggtct cctcgggtgg gggtactggg gggtccgggc ccttcgacgg
     5941 gctcggggac cttcgttgca attcagaaga acctgcgttg cggctcacgg acctcagctg
     6001 cgactccgga cccgcgctgc ggaacggagc ggtcgctgag cccgtcgagg cgcccgcgtt
     6061 cgaccccggc gggtgggtct tggacccttc gacacgctca gggggccgcg gtgcggcgag
     6121 gtaacggcat ccggaggccg gaagagacgc gcgggcccgg ccggagccgg acccgcgcgc
     6181 gggcgctcag gcccaggcga acgacgtggc cacgtggccg tggtggttcg agttcggagc
     6241 gagcaccgac tcggcggcgc gctgctcctc gactgcgacg atcgtgatct gcgacatgtg
     6301 tgtcacctcc tctcggggtc ggaatgggcg gatgggctct gggaggcgcc gtcgacgaca
     6361 gcgcgatagc gcggcgaccg cgcgcgcagc tcgtcgtgcg gtccggtgtc ggccacgcgc
     6421 ccgccgtcga cgaacagcac gcggtccatc gcccgcatga gggtcgggcg gtgggtgatc
     6481 gcgatggtcg tccgaccggc gcgcgcgaga gagagcgcgg cgaggaactc ggcctcggtc
     6541 gcggtatcga ggctcgcggt gggctcgtcg agcacgagca cagacggctc gcgcagcagc
     6601 gcgcgggcga gccccagccg ctggcgttcg ccgcccgacc aggtggctcc gcgctcggcg
     6661 agcagagtgt cgagcccgtc gggcaggcga cgcacgtgcg cggcgaggcg cgcacgctcg
     6721 agggcgtccc agagggtcgg gtcgtcggca tccggggcca gcagattgtc gcgcacgctc
     6781 gcacggaaca cgtgcggctg ctgcggcacg agcacgaccc gactgcgggt gtcggctccg
     6841 gcctcggcgg cggtgtcgac gtcgtcgatg cgcagggtgc cgcggctggc gagcagccga
     6901 gtcaggacga gggcgagggt ggtcttgccg gcgccgctgg gaccgaccac ggcgatgtgc
     6961 tcgccgggcg cgacgcgcac gtcggcgtcg acgagggccg cggatccgac gccgggatag
     7021 gcggcggtga gggcgcgggc gtcgatcgat cccggatgcc gggccccggc gcgggggtcg
     7081 gcgccggccc cgagaggggt cgggtcgccg tcgtgcggag acgggtcgac gggccccggg
     7141 tcgtgagcac cctcgcgcat gaccgggtcg accggccgca cgtccggagc ccgcagcacg
     7201 gtgtcgacgc gccgagccgc ctgggccgtc tcgcccacgc gggcgagcgc gcccgcggcg
     7261 atggccgcgg ggccgaggga cgccgcggcg aggaccacca gggtcggggc gatcgcggca
     7321 tccgctcccc cggcgacggc ggagccgacc agcagcaggg tcgtcagcag cacggcaacg
     7381 gtcatcgcgt cggtgcacgc ctgctcgacg cccgcgcgaa gcgccaggcc gcggcgtgcg
     7441 cgctgcaccg cccccgtctc gtcggccacc gcgcgggctt cggcgtcgac ccgaccgagc
     7501 aggacgacgt cccgggcggc ggagcgggcc gcgagcaccc gcgccgacac ccgcgccagc
     7561 gccgcgcgca gctcctcgcc ctgccgaccg gcgcgacgga cgaacgacag cggaatcagc
     7621 accagtgcga cctgcgcggg aacgagcagc agccacaccg gcccgaacag cacggcggcg
     7681 gtcgtgtcga tcaccgcgca cgcgagcacc ccggccgtca cctgggcgac ggtgtgggcg
     7741 gcgaaccact cgagagcctc gacgtcgccc atcgccgccg ccaccgtgtc gccggtgcgg
     7801 cggcgggcga ggccgaccgg ggccagccgg gcgatcgtgc ggtgcacgtc gaggcgcagc
     7861 gcatcgatca cgcggtaggc caacacgtgc gcgaaccact gctcgctcca ggccgcgaga
     7921 ccggcgacca cgacgctcgc gaccaccgcg gccacgaggg cgatggctgc ggcgcgcgcg
     7981 gggtctccgg cggtcacgag cgacgacgcg gccgacaccg acagcaccga cacggcgacc
     8041 gccgcgagca tgccgacgat cacgacggcg acgacgccga ggaagagtcc gcggcgacgc
     8101 cgcaggtgcg gcagcagcat cccgaggggg cggagcgcgc tcacgcgggg gctccgtgcg
     8161 cagcggggac gacggcttcg gcctcggcgg tcgggtcgcc ctccgcggcg agcagccgcg
     8221 cgtacgcccc gccgcgcgcc cgcagcacgc gcgggtggtc ggtctcgacc acgcggccgt
     8281 ccaccacgac gatcacccgg tcggcggcct cggcgaccgc gaggcggtgc gtcacagcga
     8341 cgatcgcgtc gtcggggtgt gccgcccgca gcgccgagac cagacgcgcc tcggaggccg
     8401 ggtcgagggc cgaggtcgcc tcgtcgagca cgagcaccct gcgctgctgc gcgtcgccgc
     8461 gcgcgatggc cacgcgctga cgctgcccgc ccgagagcac tccgccgccg tcgccgagcg
     8521 cggtgtcgag gtcgagcgcc gtctcaccgg tgccgatacc cgcacgggcg gcgatcgccg
     8581 acacgtcgac cgcgcgcgaa gccccggccc cgaccccgac cccgagcagg atctccgacc
     8641 ggacatcgcc ggtgaacaag gccgggtcct gcgcgacgag ggcgacccgc gcgcgcaact
     8701 gctcggcagc ggcggccgcg tccatgcggg catcccgcgc cgggctcccc ggcgcaccgg
     8761 agtcgccgac gcccgacacg cgaccgagaa tccgctcgcc gccgaaggtg atctcccccg
     8821 cctcggccgg cagcacgccc gcgaggacgg ccgcgatcga ggacttgccc gccccggtgg
     8881 ggccgacgat ggcggtcagc ccggcgtcga tccggaaggt cgcctcggtc acggcatccg
     8941 tcttcgcccc cggatgccgt gcccgaagcc cctctgcgac cagcggcagc gccaccgcgg
     9001 cgcggtcgac cgcgggcgca tcgcgccgtc cgcggtcggc tgcgacgagg tcgagcacgg
     9061 gcgggcccga gaaggtgccg aggtagcccg cgtgccaggc cgccgagagt tcccgcagcg
     9121 gccggacgag ctcgatcgac agcagcaccg ccgcgaacac cgcgaaggca cccagatctc
     9181 cgccgcgcac cgcgacgagc accagcacga gagccggcac cgcgagcagg gcgaactgcg
     9241 acaggcccga ttcgatgagc gagacgctca gctgacgcag cgtccgccgc agcagggtgt
     9301 cggacgccgc ggcgagctcg cgctcgcgtc gccgctcctg cccgaaggcg acgagcgtgg
     9361 tcatgccgtg catcgagtcg acgaactccg cgtgcagctc ctgatacgcc tcccagtggt
     9421 cgccgccgcg cttcgcgagc acgcgatccc agagccgggg cgccagcggc agaacagccg
     9481 cggccaccac ggcgacgacg ccgatgaggg ggtcgacgag caccagcgcg gtgccgacgc
     9541 cggcgaagac gatccccgtc accgcgagct gagggacgta ggacgacagg tacgcgtcga
     9601 ggttctcgac tccgtccacc acggtggcct ggtcgccgcc gctgcggccg tggccgggcc
     9661 gggtcgcgga gcgctcgatg agcgcggcga aggagcgggc cctcaggtcg cgtttgacgg
     9721 catccatcgc ccggtgcgcc actgccgctc gcacgagtgc cagcgccggg cgcacgagca
     9781 gcacggccgc gagcgccccc agcaccggca gaagcgccgc gtcgacgagc gcggacccgc
     9841 gcagcagagc cgcgaacagc tgcgacaaca gcagcgcctg agtggcccag gtgagcgaga
     9901 tcgcgacgcc gatcgcgacg agcagcacga gagcgctgcg gtggccgcgc cacagtcgga
     9961 ggaggcggag gtgcaccatg tcagcgcacc ttcttgagaa tggttatcat tacggcatga
    10021 ccgtagaagc ttccgccgac gacgtcaagt cggcgccgcg aacgccccgc cgcaccgcgc
    10081 gcgccgcggt gacgatcgcg ctgctcgtgg ctgcggtgcc gctggtggtc gtgctcggta
    10141 tggcggtcgg gcaggtcgtg ctcgcacccg gcgacgtcgt cgacgccctc ggacgggggc
    10201 tgaccggcca gcccgcgacg acggaggccg cccgcctggc cgacaccgtc gtctggcagc
    10261 tgcgcgcccc ccgggcgctc ctgggcttct gcgtcggagc gatcctggcc ctgagtggcg
    10321 cggccctgca ggccgtggtg cgcaacgacc tggcggatcc gtatctgctc ggaatcacct
    10381 ccggcgcgtc cctcggggcg gccgcggtca tcgcgctggg ggtcggagcc ctcgccggca
    10441 cgatcggcac gtcgggcggg gcgttcctcg gcgccctgct cgcgctggcc atcgtgctcg
    10501 tgctcgtcgg cccccgccgc cggctgggat cggggcggct cgtgctggcc ggcctcgccg
    10561 tcggctacgc gctgtcggcc gtcaccaacc tcatcgtggt gctctccgac aatcgggacg
    10621 cgatccgcgc catcaccttc tggatgctgg gatcgctcgg ccaggccgcg tgggacgacc
    10681 ttcccctcgt cgccgccgca ctcgtcgcgg ccctgggcgt gctcgtgatg tgggggcgac
    10741 gattggatgc catcgggctc ggcgacgacg tcgcccgcag cctcggaagc gaccccgatc
    10801 ggctgcgccg ccacgtcgcg atcgtcacgg ccgtggccat cgccgccgcc gtcgccgtca
    10861 gcggatcgat cggcttcgtc ggtctcgtcg t
//
