LOCUS       NZ_CP064276            20714 bp    DNA     circular CON 17-APR-2024
DEFINITION  Priestia megaterium strain B1 plasmid pB1.1, complete sequence.
ACCESSION   NZ_CP064276
VERSION     NZ_CP064276.1
KEYWORDS    RefSeq.
SOURCE      Priestia megaterium (Bacillus megaterium)
  ORGANISM  Priestia megaterium
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 109731)
  AUTHORS   Wang,C., Jia,Z., Zhao,J., Wang,X., Liu,Z. and Ji,F.
  TITLE     Comparative Genome Analysis of key enzyme genes in ammonia nitrogen
            Metabolism Pathway of bacteria from different sources
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 109731)
  AUTHORS   Wang,C., Jia,Z., Zhao,J., Wang,X., Liu,Z. and Ji,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2020) Guangdong Key Laboratory of Animal
            Conservation and Resource Utilization, Guangdong Public Laboratory
            of Wild Animal Conservation and Utilization, Institute of Zoology,
            Guangdong Academy of Science, Microbial Pathogenic Ecology Group,
            105 Xingang West Road, Guangzhou 510260, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. V1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 252.67x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022811885.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 10:12:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,571
            CDSs (total)                      :: 5,383
            Genes (coding)                    :: 5,338
            CDSs (with protein)               :: 5,338
            Genes (RNA)                       :: 188
            rRNAs                             :: 15, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 137
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 12 of 46
            Pseudo Genes (incomplete)         :: 26 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:16:45
            NOTE :: This is a single region extracted from a cross-origin
            section of a larger, circular record.
            Orig. start                       :: 96443
            Orig. end                         :: 7426
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP064276.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     proto_core      complement(10001..10714)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     gene            71..736
                     /locus_tag="IQ966_RS27025"
     CDS             71..736
                     /EC_number="3.1.21.-"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002040357.1"
                     /locus_tag="IQ966_RS27025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="restriction endonuclease"
                     /protein_id="WP_209369509.1"
                     /transl_table=11
                     /translation="MDKVFQGVFYLFLIMILVKLSQFAYRVLRERRYMKELAQSGMPYI
                     DKMDGYQFEIYLQALFQQLGYKPQVTKKSGDFGADLVMKGKEKIVIQAKRYGIKNRVSI
                     SAVQEVYGAKAYYKANQAWVVTNSYFTKQAKELAAACDVTLLDRADLQKFINEVNPVFK
                     AREIFENITPEPRKCPKCGQDLVVRDGNGTRFFGCSSFPSCRHTEKINKEVSKSRPSL"
     gene            778..1605
                     /locus_tag="IQ966_RS27030"
     CDS             778..1605
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IQ966_RS27030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_209369511.1"
                     /transl_table=11
                     /translation="MSSFTIYKTLCDVIDQAYPLESYPDNKFKMFFIDIKVKEMKSIHG
                     RYYPDNRKIEIFNLSRPNGHIIATTLHEVAHHIDCCIRKKSDHSKPFYEIFHQLLVTAI
                     GMGVISKQDILTARDNTDKKWLEKYFGDIEEWDISTLDYKQDSYFIKVYQSFSIKDKLK
                     NRGYKYSSLDQAWVKEMSVSEAEEEKQVVSQWIEEKNIKIEQANTIKIESYYYLCVSNC
                     YDHKAYLKENGFMWNGYGVKKAWVKKIPCQSLKLEEAKLLKLPNIKVSVAAKK"
     misc_feature    complement(780..791)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.59"
                     /tool="antismash"
     gene            1811..2371
                     /locus_tag="IQ966_RS27035"
     CDS             1811..2371
                     /EC_number="2.-.-.-"
                     /GO_function="GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /GO_function="GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013058316.1"
                     /locus_tag="IQ966_RS27035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="L,D-transpeptidase"
                     /protein_id="WP_209369513.1"
                     /transl_table=11
                     /translation="MKNIFMKSMTVLTLLMVALCGVNQGKTEAASKPANQDLIIINKYY
                     NKLAYFHDGKLEMVESVATGKTWDKTPVGFFKVVNKIKNRPYYTGHIPGGDPRNPLGKR
                     WLGLNANGTYGDTYGIHGNNNESSIGKYVSQGCVRMHNADIEKLYDKVQVGTPVAITYS
                     YKSFVDLTGVYGYKFKGYKLKNN"
     gene            2512..2712
                     /locus_tag="IQ966_RS27040"
     CDS             2512..2712
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IQ966_RS27040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_209369515.1"
                     /transl_table=11
                     /translation="MNKLEKKAIRKLKKEFEKLSQENQDQAAFYIDRQDDKHYVWDFEI
                     ENRRFEWIYVYETGVIVKSSK"
     misc_feature    2892..2907
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 19.96"
                     /tool="antismash"
     misc_feature    2892..2908
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.43"
                     /tool="antismash"
     gene            2967..3212
                     /locus_tag="IQ966_RS27045"
     CDS             2967..3212
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002130397.1"
                     /locus_tag="IQ966_RS27045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DNA-binding protein"
                     /protein_id="WP_154974434.1"
                     /transl_table=11
                     /translation="MKEIEKYMTPIEAAYVWGIRRDSLKNKFSPSKLNQEKEEELNQMI
                     EEGLIKFFLHPDRERKEWIISREAMIKWFGEPNSKN"
     gene            3233..3511
                     /locus_tag="IQ966_RS27050"
     CDS             3233..3511
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IQ966_RS27050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_209369517.1"
                     /transl_table=11
                     /translation="MIGSVFLSGMTDVVQNFLILVIVIGAALALIPVIGYLGTWAFNGK
                     QMKKEWSLFNEEEKKQEQLNVLKYVMPFIIVAAVIFVFISSTIFMGN"
     gene            complement(3918..4217)
                     /locus_tag="IQ966_RS27055"
     CDS             complement(3918..4217)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014460615.1"
                     /locus_tag="IQ966_RS27055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_098999908.1"
                     /transl_table=11
                     /translation="MEKFFKNALKVSIASLVISITLYVVIIGLFLLSVVTANIFGTVIR
                     IIVPILTLVTILGFIYSFFIKGKQKIPFLTLHFVSLCALSFGIYLAVSFQGFAP"
     gene            4400..5011
                     /locus_tag="IQ966_RS27060"
     CDS             4400..5011
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014460613.1"
                     /locus_tag="IQ966_RS27060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_209369519.1"
                     /transl_table=11
                     /translation="MDIEVGFLSKSFISDIPSWGGLFPFGIDGDAPYIVEYEGHDVDIE
                     TFINNWMKELNRFPIYVLFTYISYDAEEIGDVFSSANIEYVKLKVDRETFIKAKIKDYT
                     QFKVVMPFLYSQGSMNCFAAWSLSEDLFSFGKRDMKTLFGRKKVQMPIVTLRKDCTVFW
                     IFYDGASVVSLSNDDLFSSTNSIISSFPQNIKGAEVEYED"
     gene            5158..5739
                     /locus_tag="IQ966_RS27065"
     CDS             5158..5739
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1058: ArsR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013060163.1"
                     /locus_tag="IQ966_RS27065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_098113790.1"
                     /transl_table=11
                     /translation="MQLDKLVNYHKAVGDPTRIRIISLLKKGPLHGQAIAYKLGLQPPT
                     ITHHISKLRDVGLVYQRRQGNIIYFYLDSERLEYNSKAILNIGEESMENQDSKINNQDK
                     LSIVKNFINSDGTLKQIPSQRKKKNVILAYLIRDLHQGKTYKERDLNEYIKNYHEDYAT
                     IRRELIMQHFMYRQNGEYELNPKEMWPVTF"
     gene            5832..7058
                     /locus_tag="IQ966_RS27070"
     CDS             5832..7058
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013060162.1"
                     /locus_tag="IQ966_RS27070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_098433885.1"
                     /transl_table=11
                     /translation="MIPITKEAKVQTGIWKGKFIYLWVASIFSSLSISMYLTIEQWYVV
                     NYLNLKSSLGIILMATTIPRVLLMVIGGVVSDKYRRSKVIFFSLLTRCFMLLLMALFYT
                     NNLLSLSVLFIFALLFGASDAFFWSARDSIVPGIVHKEQLVRANSVIQTTNQLSVMFGP
                     VLGATLLSFFSYSTIFLVIAFTLLISIILLQFIKEKKTLRPKKTSILRDLAEGINYVKD
                     SSFLLTVMVTFIIVNLFFIGPLMLSIPILAEDRFKGNAFYLSILQSSFASGMLLGAIIM
                     GWLNIKRNRGKLAISFIFLEGLLLMIFSREIDLWLAAAILLAIGCCVSSINIPIVSLIQ
                     EKTPSTIVGRVMSVNTMVSMGLIPVSYGLVSTLLSINVDIKFILLCAGIVIVLFSVLLL
                     LKASDLKNT"
     gene            7218..7796
                     /locus_tag="IQ966_RS27075"
     CDS             7218..7796
                     /EC_number="4.2.1.-"
                     /GO_function="GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009673318.1"
                     /locus_tag="IQ966_RS27075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DJ-1/PfpI family protein"
                     /protein_id="WP_209369522.1"
                     /transl_table=11
                     /translation="MKVGFILFDQLTALDFIGFYDGITRLKTLGFKKDLTWDLCALNNE
                     ISDDRGITFKINKVSPDLSEYDLIFVPGGMGTRSLQYDTEFINWLKTAKDVPYKVSVCT
                     GSLLLGAAGFLTNIKATTHPNGFDLLKQYTDKVREERIVRDSNVITGGGVATSVDLGLY
                     ICELIDGAETATKIQKQMDYPYYKTGIMN"
     gene            8209..8673
                     /locus_tag="IQ966_RS27080"
     CDS             8209..8673
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003185275.1"
                     /locus_tag="IQ966_RS27080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_182031417.1"
                     /transl_table=11
                     /translation="MEALQKQAFQAIECLIMTREKKVQSRKVLANKLTQSVNDTNKKWT
                     ITQLHIVSLINKDKYVNNAFLSKELNISKAAISKAINTLLQYGILTAHKNANNNRETFY
                     SLTEEGEKLATIHDKMHEIAKERYEELLNKFSDVELKIIIKFLNEWTKQI"
     misc_feature    complement(8223..8236)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: strong, score: 23.52"
                     /tool="antismash"
     gene            complement(8744..9967)
                     /locus_tag="IQ966_RS27085"
     CDS             complement(8744..9967)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010332029.1"
                     /locus_tag="IQ966_RS27085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome P450, cyclodipeptide
                     synthase-associated"
                     /protein_id="WP_154974526.1"
                     /sec_met_domain="p450 (E-value: 1.7e-40, bitscore: 131.9,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTSFKTFSILSEQFHQDPYRYFSELRDFDPIHYEQNIDSYFISR
                     YKDVRYVLQNTEIFTTKSLAKRAEPVMRGPVLAQMKGKEHTAKRKIVVKSFIGDSLKRL
                     IPLIKENAEVLLSPHLSKGRIDLVNDFGKTFAVYVTMDMLGLNKKDHQLIADWHSGVAN
                     FITTIQLSSEERDHSLWCSEQLANYLLPIIEERRQNPKSDLISILCQSEYEGVEMSDTN
                     ILALILNVLLAATEPADKTLALLMYYLLNNPLQMQDVMEDRSLLSRAIAETLRFKPPVQ
                     LIPRQLSQDAKIADKLLPKGSTVFCMIGAANRDPEAFDNPDTFNIHRPDLDVKTAFTGI
                     ARHVAFGSGIHNCVGAAFAGTEIEVVANIVLDQLKNMKLEEGFQYKESGLYTRGPVSLP
                     LTFDPIKE"
     misc_feature    complement(9985..10000)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 24.77"
                     /tool="antismash"
     gene            complement(10001..10714)
                     /locus_tag="IQ966_RS27090"
     CDS             complement(10001..10714)
                     /GO_function="GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010328738.1"
                     /locus_tag="IQ966_RS27090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tRNA-dependent cyclodipeptide synthase"
                     /protein_id="WP_209369524.1"
                     /sec_met_domain="CDPS (E-value: 1.9e-73, bitscore: 239.5,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQILLEKIAKPLFAIEPLSQNCQRIFERRNHILIGISPFNSRFS
                     EEYIYRLIEWSIKEFKDVSVLLAGNEAANLLEALGTSRVKAERKVRKEVNRNRRFAERA
                     LKAHGGNPEAIYTFSDFLTQPVYCDMRTKIEESFLHQPNFQNVCLEMSHAAIVGRASGT
                     NMDISVITSEMLHNAVQYVLAELPFFIGAPNILGIQETVLAYHKPWKLGEQIKDEQFSI
                     RMQQNQGYITVDEIH"
     misc_feature    complement(10674..10694)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 28.67"
                     /tool="antismash"
     gene            10983..11468
                     /locus_tag="IQ966_RS27095"
     CDS             10983..11468
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032352.1"
                     /locus_tag="IQ966_RS27095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_154974523.1"
                     /transl_table=11
                     /translation="MSLLSKQLIYENYLQLLHLNEQKADSVSQTFFDYLDEKELNEIGT
                     LPSNMSSIHVIECIGNNGPINNIGIATRMNLSKANITKITRTLTKDELIKRFQLADNKK
                     EVYFKLTAKGKQVFELHEKLHSKRKRQFYDLIDDFSDDKQKIILEFLETMINKLRQE"
     gene            11542..12756
                     /locus_tag="IQ966_RS27100"
     CDS             11542..12756
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082482.1"
                     /locus_tag="IQ966_RS27100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_209369526.1"
                     /transl_table=11
                     /translation="MGHTNTKPIFILYVVCISAFFASLNQNIYSPIIPLIKDSFDVSTT
                     LVNLTVSLFIFITAIMQIVYGTILDFKGSRFILLPSLMLTVLASIGCAVTSNFTLFLVF
                     RSLQALGTAAIPLIAAATITDFFDGEERGSAMGTYQTLLSIAPAVAPILGGFIGDRYNY
                     DGVFWSLVGISILLLIINMLFFPKDQKKHKASKRNGLHFYLVMAKDKMGSVLLSLSFLN
                     FLIYFSILVYLPVLLTDHYHLNLKLVGLLYLPMTLSLIAGSLLFKYIQKKISLINIFTF
                     TNFLIVFSVIGFGLTHTVSLLLMSLSLFIYGVGIGILTPSYATILTHQFEQNRASAMGV
                     FNFVRYTGMSIGPILSGTLLEFLRSETMFIMFGVMFGIISIFGYLLVDNPKNTGENRTT
                     NKPSF"
     misc_feature    13000..13014
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 22.73"
                     /tool="antismash"
     region          1..20714
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="CDPS"
                     /region_number="1"
                     /rules="CDPS"
                     /tool="antismash"
     cand_cluster    1..20714
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="CDPS"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20714
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[10000:10714](-)"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     gene            13392..15317
                     /gene="thrS"
                     /locus_tag="IQ966_RS26545"
     CDS             13392..15317
                     /EC_number="6.1.1.3"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="thrS"
                     /gene_functions="other (smcogs) SMCOG1162: threonyl-tRNA
                     synthetase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014460609.1"
                     /locus_tag="IQ966_RS26545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="threonine--tRNA ligase"
                     /protein_id="WP_209369529.1"
                     /transl_table=11
                     /translation="MSKDLIQIRFLDGNEKEFSKGITLHAIAESISSSLKKKAIAGKVN
                     GELYDLHRAINQNAEIEILTVDSDEGLEIMRHTTAHILAQAVKRVHKNVKLGVGPVIED
                     GFYYDMDLFTTIASNELLQLEHEMKKIINENHPIERTEVTRSEAERIFSEINDELKLEL
                     LRDIPMNETVTIYKQGEFFDLCRGPHLPSTGMVKAFKLTKVSGAYWRGNSDNQMLQRVY
                     GVAFRSQKQLNEYLMFLEEAAKRDHRKLGNELELFMFSDEAPGMPFYLAKGQMVKNQLE
                     QFLRSVQEKHNYEEVRTPFMMNQQLWEKSGHWDHYKDNMYFSEVDNAKFALKPMNCPGH
                     MLIFKNKLHSYRDLPIRMGEFGQVHRHEFSGALNGLLRVRTFCQDDAHIFVTPEQIESE
                     IKSVIELVDYIYKTFGFEYSIELSTRPEDSMGEDSLWELAENSLENVLRKLNFDYKLNE
                     GDGAFYGPKIDFHIKDALKRSHQCATIQLDFQLPEKFDLNYINEKNEKQRPVVIHRAIF
                     GSLDRFFGILIEHFGGVFPTWLAPVQVKVIPVSLKVHQEYAQEIKEALGKKGIRVELDN
                     REQKLGYKIREAQVAKVPYILVLGDKEMESNSVNVRKHGEEKSETFNLDSFIEKLQDEI
                     VEKKLR"
     gene            15530..16519
                     /locus_tag="IQ966_RS26550"
     CDS             15530..16519
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) fabH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013059995.1"
                     /locus_tag="IQ966_RS26550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="WP_209369531.1"
                     /sec_met_domain="fabH (E-value: 2.3e-98, bitscore: 322.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSTARITAIGTYVPEKRLTNDDLERIVDTNDEWIVKRTGMKERR
                     IADENEYSSDLAIKAIENLVEQYQKELKDVGCIVVSTTTPDYAFPSVGAQIQDYFDIPT
                     TGALDFNATCAGFTYGLHMANAFITSGLHDKVLVVTAETLSKVTDYTDRTTCVLFGDGA
                     AAVLVENDAERPSFIATHIGTNGKGGKHVYRTNLSTTMHGQPLSSSGKMVQNGREVYKW
                     ASRTIPLGMAELLEQANLQKSEVNWFVPHSANLRMVESICERSEFPVEKTLTSVEYMGN
                     TSSVSIPLALQIGINEGKLKDGDVLLLYGFGGGLTHAGHIIKWDLGKE"
     gene            complement(16758..17243)
                     /gene="coaD"
                     /locus_tag="IQ966_RS26555"
     CDS             complement(16758..17243)
                     /EC_number="2.7.7.3"
                     /GO_function="GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="coaD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) CTP_transf_like"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942871.1"
                     /locus_tag="IQ966_RS26555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="WP_047752015.1"
                     /sec_met_domain="CTP_transf_like (E-value: 1.8e-21,
                     bitscore: 69.7, seeds: 65, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAIYPGSFDPITNGHIDIINRASEFFDEILVAGLVNSKKQYLFS
                     TEERRQLIEQSFKDNKKVKVDTFEGLLVDYMKEHNYKAIIKGLRTVTDYGYEAQMALTN
                     KNLYKEAETFFIHSEEKYSFLSSSMVKEIYSNGGRLSSYIPEHVEKALLLKVPCYQG"
     gene            complement(17453..18094)
                     /locus_tag="IQ966_RS26560"
     CDS             complement(17453..18094)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF044761.1"
                     /locus_tag="IQ966_RS26560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_168761487.1"
                     /transl_table=11
                     /translation="MKRIVKKPEERRIEIITVAKGLFSSNGYENTTVNDIIKKVGVAKG
                     TFYHYFKSKDEIADAVIQDSIDSSVQLFKQIINNPDLKAIEKFISIIQFFSQETSNHFS
                     DGLMTHLHHKDNVLLHQKMKVGMIKEYAPIISSVVKQGVEEGVFHTNFPDEITEFLLVG
                     LHFMLDPSLFSLTHEQLIAKLDAIDEIYEKLLGAPKGSFPSIKQYFEKLY"
     gene            18205..20553
                     /gene="helD"
                     /locus_tag="IQ966_RS26565"
     CDS             18205..20553
                     /codon_start=1
                     /gene="helD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007087203.1"
                     /locus_tag="IQ966_RS26565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="WP_209369533.1"
                     /transl_table=11
                     /translation="MTNGNQERKYENERIRIVLREIDKKVVKLDYDSGGLKKDVIHLKK
                     TFWDDVKINLDNPEDAGETLSSIRQQAEMLSERERTHRQLDKQRKTLSRLKDSPYFARV
                     DFLENGEETTNNVYLGVASLMNEKEEKFLIYDWRAPISSIYYDYSPGPAQYETVEGMIQ
                     GEMQLKRQFITKDGQIKTMFDTGVTIGDELLKEILGNNASINMKSIVATIQKEQNQIIR
                     NVKSKYLVVQGVAGSGKTSAALQRVAYLLYRYQDEITAENIMLFSPNSLFNRYIASVLP
                     DLGEDNMQQLTFQEYVEKRLGKQFDVEHPFTQMEYMLTCQEHKMYSARRKGIRYKASND
                     FKSLIDSYVVVLSSEDYLIFKNISFRGEMLIGAEQIKSYFYSLDASISIPNRMQLVKEW
                     LLKELVKKEKLERTKDWVIEESDLLDLEEYTKVYSELLKEGRFSENTFDDFEQEQKKIA
                     KKIVKKHFKPIRTAVKKLKFINVAALYSQLFKQKDNIYQKLNDTFPEGWEEICSYTLKK
                     LHRKELTYEDAAPYLYLQDKLEGKRINTRIRHLFIDEAQDYSPFQFFVLKQLFPYCRMT
                     ILGDFNQAIYAHTLNAPTLLSNELYVKENFEKVVLTKSYRSTRQIVEFTKGIIEEGETI
                     EAFNRNGQKPTFTKVEDLDTLQSRITKKIRQLQSANHETIAVICKTGKESWEAYDRLSE
                     QINIQLMEHNTSSYRKGILILPAYLAKGIEFDAVIIYDASDVTYGREFERKLFYTACTR
                     AMHELHLFSVGEMSPFMNKIPANSYEITE"
ORIGIN
        1 aatggaaaaa gcattcaacc tgtggcatca cgtctccgga ataaacaaac tgaacaggag
       61 ctttcaatga atggataaag tgtttcaagg tgtattttac ctctttctta tcatgatttt
      121 agtgaaactt tctcaatttg cttaccgtgt ccttcgtgaa cggaggtata tgaaagagtt
      181 agcccaatcg ggtatgccct atattgataa aatggatgga tatcagtttg aaatttattt
      241 gcaggccctt ttccaacagt taggatataa acctcaagta accaaaaagt ctggtgactt
      301 tggggctgat ttagtgatga aaggcaaaga aaaaattgtc attcaagcta aacgatatgg
      361 aattaaaaat cgggtcagta tttcagctgt acaagaagtg tatggggcaa aagcgtatta
      421 taaagcgaat caggcgtggg ttgtgaccaa tagttatttt acgaaacaag cgaaagaact
      481 tgcagctgca tgtgatgtga ctttattgga tcgggcggat cttcaaaagt ttattaatga
      541 ggtaaacccc gttttcaagg cacgggaaat ctttgaaaat atcacaccag aacctcgtaa
      601 atgtccaaag tgtgggcaag atctagtcgt aagagatggg aatgggactc gcttttttgg
      661 ctgttcctct tttccttctt gtcgtcatac ggaaaaaatt aataaagaag taagtaaaag
      721 cagaccttca ttatagtaag gtctgttttt ttctttctct aaaaaggagc cgatgttatg
      781 agttcattta ccatttataa aacgttatgt gatgtaatcg atcaagctta tcccttggaa
      841 agttatcctg ataataaatt taaaatgttc tttatcgata tcaaagtaaa agaaatgaag
      901 tccattcatg gacgatatta tccagataac cgaaaaattg aaatctttaa tctttcaaga
      961 cctaatgggc acattattgc gacgactcta catgaagtcg ctcatcatat tgattgctgc
     1021 attcgcaaaa aaagtgacca ctcaaagcct ttttatgaga tttttcatca gttacttgta
     1081 acagctatag gaatgggggt tatatcaaaa caggatatct taacagcacg tgataataca
     1141 gataaaaaat ggctagaaaa gtattttggt gacatcgagg aatgggatat ttcaacttta
     1201 gattataagc aagattctta ctttatcaaa gtttatcagt ccttttctat taaagataaa
     1261 ctgaaaaatc ggggctataa atatagctcc ttagatcaag cttgggtgaa ggaaatgagt
     1321 gtttctgaag ctgaagaaga aaaacaggtt gtatctcaat ggattgaaga aaagaatatt
     1381 aaaattgagc aggccaacac gatcaaaatc gagtcttatt attatttgtg tgtgtccaat
     1441 tgttacgatc ataaagctta cttaaaagaa aacggcttta tgtggaatgg ttatggagtg
     1501 aaaaaggctt gggtcaaaaa gattccttgc caatccttaa aattggaaga agcaaaactt
     1561 cttaagctgc ctaatattaa agtatctgta gcagcaaaga aatgacgctt tttctaggtg
     1621 ctgaaatttg catcgaatga gtagttatac gcaaaacaaa agttaatttc gtcagagagc
     1681 gtttctctgc atgtcaaaat cgtcgattaa ctaggttttt atttataaat aattgtgacg
     1741 aaacagcact ttctaattag aaggtgctgt tttcgtttct tataaaaata taaaaaagag
     1801 gagagttttt atgaagaaca tttttatgaa atcaatgacc gttcttacat tattaatggt
     1861 cgcattatgt ggagtaaatc aggggaaaac agaagcagca agtaagccag cgaatcaaga
     1921 tttaatcatt atcaacaaat actacaataa gcttgcgtat ttccatgatg ggaaattaga
     1981 aatggtagag tcagttgcaa cgggtaaaac atgggataaa acacctgttg gcttttttaa
     2041 agtggtaaac aagattaaaa accgccctta ctatacagga catattcctg gtggcgatcc
     2101 tcgcaatccg ttaggaaaac gttggttagg actaaatgca aacggtacgt atggtgatac
     2161 atacggcatt cacggcaata acaatgaaag tagcattgga aaatatgtga gtcaaggttg
     2221 cgtgagaatg cataatgcag acatcgaaaa gctttatgat aaagtccaag taggcacacc
     2281 ggttgccatt acgtattcct ataaaagctt cgttgatctt acaggcgtat acggttataa
     2341 attcaaaggc tataagctga aaaacaatta atatctgagc agcattccat gtttttttat
     2401 catggagtgc tgcctttttt cattgataaa atgtttattt tcccctactt tacccttgta
     2461 agggtaaagt aggaagtgta taatatcctt ataagggttg gaggtgataa catgaataag
     2521 cttgaaaaaa aggctataag aaaattaaag aaagagttcg aaaagctatc tcaagaaaat
     2581 caggatcaag cagcgtttta catcgatagg caagatgata agcattacgt ttgggacttt
     2641 gaaattgaga ataggcgttt tgaatggatt tacgtctacg agacaggggt catagtgaag
     2701 tctagtaagt gaattatcct tgaatctaac tatggaggag atgttaatat gaagatatct
     2761 actaaagaat ggttaacatt aagtgcagaa attcgtgaaa agttgattag acttgcattc
     2821 ttagagaatc agaacaaaat aaataccctt cgctaacttg ggacattagc aaagggcaat
     2881 gtgaaacgtt aataccgcaa gagggaatat agcccattcc cttttgctct tcttttttag
     2941 aataacataa aaaggagaac attacagtga aagaaataga aaaatacatg acaccaattg
     3001 aggcagctta tgtttggggg attagacgag atagcttaaa aaataaattt agtccttcaa
     3061 aactaaacca agaaaaagaa gaagagttaa atcaaatgat tgaagaagga ttgattaaat
     3121 tttttctcca tccagataga gaacgaaaag aatggatcat cagtagagaa gccatgataa
     3181 agtggtttgg agaacctaat agtaagaatt aatctttaaa ggagatgagt gaatgatagg
     3241 aagcgtcttt ttaagtggta tgactgacgt tgttcaaaac tttttaattt tagtcatagt
     3301 aattggggct gcgctcgctt tgatacctgt tattggctat ctaggaacat gggctttcaa
     3361 tggaaagcag atgaaaaaag aatggtcttt attcaatgaa gaagaaaaga aacaggaaca
     3421 attaaatgtg cttaaatatg tgatgccgtt tatcatagtt gcagctgtga ttttcgtttt
     3481 tatctcgtcc actattttta tggggaatta attttttaaa aatataacta caaataaaaa
     3541 accttagcag aactaaggtt tttttattta agtaaattca ttaatttctt ttattccttt
     3601 aagtaatctt taaagtactt tatttaaaat acttttatca tttacttatg ttgtagaaaa
     3661 ataaccaatt tgtcgtcact aactgtaaat agtaaaacat taataataaa tgaagtaatt
     3721 taatatttac ttaacaaata ataaaacgta aacaaggtta tttactctaa atatagtgaa
     3781 agacaacatt ttttaaaagg tatatgtata tgaatctata aatctggcca gactaagagt
     3841 gtattccccc tattccgata aatttgaaaa ccctttgcct atatgagcaa agggttcttt
     3901 ttttatgtaa atatttatca aggcgcaaac ccttgaaaac tgactgcaag atagatacca
     3961 aacgaaagag cacataagga aacaaaatgt aatgttaaaa aagggatttt ctgtttacct
     4021 ttgataaaga aggaataaat aaacccgagt attgtaacta aagttaaaat aggcacaata
     4081 atcctaatca cagtaccaaa aatatttgct gtaacaactg ataaaagaaa caatccaatt
     4141 ataactacat ataaagttat cgaaatgact aaagaggcta ttgatacctt taaagcattt
     4201 ttaaagaatt tctccatcta atttcctcct atttttagca acgcatattc attataatat
     4261 acaggttaaa aggaaataag taccttttct tgaagtgcat tatatttcta gttaccataa
     4321 cgttaattat aggaacttat ttacatatcg attataatta tacttaaaat acactttaag
     4381 tattgaagga gaatatataa tggatattga agtagggttt ctttccaaaa gcttcataag
     4441 tgatattccc tcatggggtg gtttgtttcc cttcggaata gatggcgatg caccttacat
     4501 tgtagagtat gaaggtcatg atgtagacat agaaaccttt attaataact ggatgaaaga
     4561 gttaaacagg tttccgatat atgtactttt tacatatata agctacgatg cagaagaaat
     4621 aggtgatgta ttttcatctg ctaatattga atatgtaaaa ctaaaagtag acagagagac
     4681 ttttattaaa gctaaaatta aagattatac acaatttaaa gtcgttatgc cttttttata
     4741 tagccaagga agtatgaatt gctttgctgc ttggtctctt tctgaagatt tatttagctt
     4801 tggaaaacgg gatatgaaaa ccttatttgg gagaaaaaag gttcaaatgc ctattgttac
     4861 attacgaaaa gattgcacag tgttttggat tttttacgat ggtgcatctg ttgtttccct
     4921 atctaatgac gatttatttt cttcaactaa ttcaattatt agctcctttc cccaaaatat
     4981 taaaggggct gaggtggaat atgaagattg attcttatat cttataagct ttacaaatct
     5041 aattaccata atccatatta taggaaatta tcaatctggt actttttgac acagacctcc
     5101 aattcgatta ttatctaatt agataataat cgaattggag gaggaaggta ttaaattatg
     5161 caactagata aattagtcaa ttaccataag gcagttggag accccacaag aattagaatt
     5221 atttccttat taaaaaaagg ccctcttcat gggcaagcaa tcgcttataa acttgggtta
     5281 caaccgccaa ccattactca ccatatttca aaattgagag atgtagggtt agtttatcaa
     5341 agaagacaag ggaacattat ttatttttat ctcgatagtg aaaggctaga atataattca
     5401 aaagctatct taaatattgg ggaggagtct atggagaacc aagatagtaa aatcaataat
     5461 caagataagt tatctattgt taaaaatttt ataaatagcg acgggacatt gaaacaaatt
     5521 ccgagtcaaa gaaagaaaaa gaacgttata ttagcgtatt taatacgtga tttacatcaa
     5581 ggaaaaacgt ataaagaaag ggatcttaat gaatatataa aaaactatca tgaagattat
     5641 gcgactattc gtagagaatt aattatgcaa cacttcatgt ataggcaaaa tggagaatat
     5701 gaattaaatc caaaagagat gtggcctgtt accttttaaa atgaacgttt cttcgttcat
     5761 ttttcaatca atctaattcg atatctatct aattagatta ctgttatttt taataagaat
     5821 gaggtgtaaa tgtgattcca ataactaaag aggctaaagt acaaacaggt atttggaaag
     5881 gaaagtttat ttatctatgg gtagcaagta tattttcaag cctttctatt tcaatgtatt
     5941 taactattga gcagtggtat gtagtcaatt acctaaactt gaagagctct ttaggaatta
     6001 ttttaatggc gactacgatt cctagagtat tattaatggt cataggaggc gttgtatcag
     6061 ataaatacag acgatctaaa gttatattct tttcattact aactaggtgt tttatgctac
     6121 ttctaatggc tttattctat acaaataatt tattatctct ttccgtttta tttatatttg
     6181 cattattatt cggggcatct gatgcctttt tttggtcagc tagagattca attgttccag
     6241 ggattgttca taaagagcaa ctagtcagag cgaattcagt aatacaaaca accaatcagc
     6301 tttctgtcat gtttggccct gtcttaggtg ctactctcct ttcatttttc tcatattcaa
     6361 ccatctttct ggtaattgcc tttacacttt tgataagcat aatcttgtta caattcatca
     6421 aagaaaagaa gacattaagg cccaagaaaa catctatctt aagggattta gctgaaggaa
     6481 ttaattatgt taaagattcc tcgtttcttc tgacagtcat ggtgacattt attattgtca
     6541 acttattctt tattggacct ctcatgttaa gcatccctat ccttgcagag gatcgtttta
     6601 aaggaaatgc tttttactta agtattttac aaagttcgtt tgctagtggc atgttgttag
     6661 gagctataat catgggctgg ttgaacataa aaaggaatag aggaaaactt gcaatttctt
     6721 tcatattctt ggaagggtta ttacttatga tatttagccg agaaattgat ctctggcttg
     6781 ctgctgctat tttgttggct attggatgtt gtgtatcgag tattaatatt cccatagtta
     6841 gtttaataca ggagaaaaca ccctcaacca tagtaggtcg agttatgagt gtaaatacaa
     6901 tggtttccat gggacttatt ccagtttctt atggattagt ttccactctt cttagcatta
     6961 atgtcgatat taaatttatc ctcttatgtg ctggtatcgt gattgtatta tttagtgtac
     7021 tacttctttt aaaggcttca gatcttaaaa atacatgagg catctacaat aaaaccaggc
     7081 cttatttagt caccataatc cagattctag gaacagatat atctggttat atgacaaact
     7141 atcaagaata agagggtatt agaaaaatgt ggagaattaa attacattat taattatgtt
     7201 aaagaaggtg taatttaatg aaagttggtt ttattctttt tgatcaatta acagctttag
     7261 actttattgg gttttacgat ggcattacac ggttaaaaac actaggattc aaaaaagatt
     7321 taacgtggga tctttgtgca ttaaataatg aaataagcga tgaccgtgga attacattta
     7381 aaataaataa agtttcacca gacctatcag agtatgattt aatctttgtg ccaggtggaa
     7441 tgggtacccg atctttgcaa tatgatactg aattcattaa ctggctaaaa acagcaaaag
     7501 atgttcctta taaagtttct gtgtgtactg gatcacttct actaggagca gcaggcttcc
     7561 taacaaatat aaaagctact actcacccaa atggatttga tttgttaaaa caatatacag
     7621 acaaagtaag agaagaacga attgttagag attccaatgt tattactggt ggtggagtag
     7681 ctacttcagt agatctagga ctctatatat gtgaattaat tgatggcgca gaaactgcaa
     7741 cgaaaattca gaaacaaatg gactaccctt attataaaac tggtattatg aattaaaatc
     7801 aacaccagaa gccttcaaga tgaaagcttt tggtgttgat taacgtccgc ctttcgctat
     7861 gtatagcata ttcattttta gttaacataa tcactctttc cggaagacat accaaaaacg
     7921 agtccttggt aaacaagggc tcgtttgctt tttcatttta tcggttatgc agccttttat
     7981 acatcgttga tatgacggag ttctctttat aggtaattga ggagatgatg tataaaaagg
     8041 ggactattta tacatcattt ttttataaat aaagatagta taagctacca agaagttata
     8101 atatagaaaa agactaaact aaatgaactt gacaacaaaa ttgttaacca gtaaactatt
     8161 ttatatagtt aaccagttaa caattatatt aaagaaggtg ggtttcgtat ggaagctctt
     8221 cagaaacaag cgtttcaagc tattgagtgt ttaattatga ctagagaaaa aaaggtccaa
     8281 agtaggaaag tgttagctaa caaactaact caaagtgtaa atgatacaaa taaaaaatgg
     8341 acaattactc aattacatat tgtttcatta ataaataaag ataagtatgt aaacaatgct
     8401 tttctttcta aagaacttaa tatctcaaaa gctgcaatat ctaaggctat taatactctt
     8461 ttgcagtatg gcatccttac ggctcacaaa aatgctaata ataataggga aaccttttac
     8521 tctttaacag aggaagggga aaagttagcc actattcatg ataaaatgca tgaaatagca
     8581 aaggaaaggt atgaagaatt acttaataag ttttcagacg tagaattaaa gataattata
     8641 aaatttttaa atgagtggac caaacaaata tagaaatagg cttacgacat gcctaatgag
     8701 cacgccgtaa gtccacttat ctaattgtct gaaagttaac aacttactct ttaataggat
     8761 caaacgtcaa tggaagagaa acaggtccac gtgtataaag accactttct ttgtactgga
     8821 aaccttcttc taatttcata ttttttagtt gatctaaaac aatgttagct actacttcaa
     8881 tttctgttcc tgcaaatgct gctcctacac aattatgtat gccagaaccg aaagccacat
     8941 gtcgagcaat acctgtaaac gcagttttaa cgtcaaggtc tggacggtga atgttaaaag
     9001 tatcaggatt atcaaatgct tcaggatctc gattggcagc tccaatcata cagaatactg
     9061 ttgaaccttt aggtagtaat ttatcggcaa ttttcgcatc ttgcgataat tgccttggta
     9121 taagttgtac tggtggttta aaccttagag tttcggcaat tgctcttgat aataatgaac
     9181 gatcttccat cacatcttgc atttgtaacg gattatttaa taaataatac atcaataaag
     9241 ccaatgtctt gtctgcaggt tcggtagctg ctaatagtac attaaggata agtgccaaaa
     9301 tgttcgtgtc ggacatctca actccttcat actcagactg acataatatt gaaatgagat
     9361 ctgattttgg gttttgtcga cgctcttcta taatgggtaa aaggtagttt gctagttgtt
     9421 cactgcacca taatgaatga tcacgttctt cagatgacaa ctgtatagta gtgataaagt
     9481 tagcaactcc actatgccaa tctgcgataa gttgatgatc ttttttgtta agtcctagca
     9541 tatccatcgt gacatataca gcaaaagttt ttccaaaatc gttaactaag tcaattctac
     9601 ctttacttaa gtgtggtgat aataacactt ctgcgttttc tttaattaaa ggaatgagtc
     9661 tcttaagtga atcacctata aaactcttta ctacaatttt acgttttgct gtatgttcct
     9721 ttcctttcat ttgtgcaagg acaggtccac gcattacagg ttccgcacgt ttagcaagag
     9781 atttagtcgt aaagatttct gtattctgta atacatacct cacgtcttta tatcggctaa
     9841 taaaatagct atcaatattt tgctcataat ggataggatc gaaatcgcga agttctgaaa
     9901 aatatcggta tggatcttga tgaaactgtt ctgataagat actaaaggtt ttaaatgatg
     9961 tattcaagac ggttcctcct aaaagtttaa aggtgtaaat ttaatgaatt tcatcgacag
    10021 ttatatatcc ttgattttgt tgcattctaa ttgaaaattg ctcatctttt atttgttcac
    10081 ctagtttcca aggtttatgg taagcaagta cagtttcttg aatacccaaa atattaggtg
    10141 ctccaataaa gaacggcagt tcagccaaaa catattgcac agcattatgt aacatttctg
    10201 aagtaataac tgaaatatcc atatttgttc cagatgctct tcctacaatt gctgcatgtg
    10261 acatttctaa acaaacattt tgaaagtttg gttgatgtaa aaaactttct tcaatcttag
    10321 tgcgcatatc acaatatacc ggttgagtaa gaaagtctga aaatgtataa atagcttcag
    10381 ggttaccacc atgagcttta agagctcttt cggcaaatcg acgatttctg tttacttctt
    10441 ttctcacttt acgctcagct ttcaccctag aagttcctaa agcttctaaa aggtttgcgg
    10501 cctcatttcc agctaagaga acagagacat ccttaaactc tttaatgctc cactcaatta
    10561 gtcgataaat atattcttct gaaaatctac tgttaaaggg gctaattccg attaatatgt
    10621 ggtttcttct ttcaaaaatt ctctggcaat tttgtgataa gggttcaata gcaaataaag
    10681 gcttagctat tttttctaaa agaatttgtg acatagctat ttcaactcct aggattaaac
    10741 gattaatgtt cactagtaaa caaaaactat tttaaatcca aatatttgta aaagtcaacg
    10801 ttttttcttt attttgtaaa tttataagcg tttttcctgc aaatatagta ggttttaatt
    10861 actatgaaat ctgattttat tgtatagaaa agagatttta cattcattta atagcttatt
    10921 agtagtataa tgtttactgg tgaacattaa ttatttatac attagaaagg taaggaacta
    10981 atatgtctct attatctaaa cagttaatat acgaaaatta tttgcaatta ttacacctga
    11041 acgaacaaaa agctgattca gtttcccaaa cattttttga ttatcttgat gaaaaagaac
    11101 tgaacgaaat aggaacatta cccagtaaca tgagcagcat ccacgtgatt gaatgcattg
    11161 gaaataatgg acctataaat aacataggga ttgcgactag gatgaattta tctaaagcaa
    11221 atatcacgaa aattacccga acattaacga aagatgagtt aattaaaaga ttccaattag
    11281 cagacaataa aaaagaggtc tactttaagt taacagcaaa aggaaaacaa gtttttgaac
    11341 ttcacgaaaa actacatagc aaaaggaaac gacaatttta tgatttaatt gatgatttct
    11401 ctgatgacaa acaaaaaatt atattagagt tccttgaaac aatgattaat aaattaagac
    11461 aagaatagac aaattagttg ctcttagaat gtcaagcata cttttatatg gaaaaactta
    11521 tttacaaggt aggagattta tatgggacat acaaatacaa aacctatttt tatcttatac
    11581 gtcgtttgta taagtgcatt ttttgcatct ttaaatcaaa atatttactc gcctattatt
    11641 ccgttaatta aggattcatt tgacgtgagc acaacattag ttaatttaac tgtttcattg
    11701 ttcatcttta ttacagctat tatgcaaata gtctacggga ctattcttga ttttaaagga
    11761 agtcgtttta ttcttctccc tagtcttatg cttaccgtat tggctagtat tggttgtgct
    11821 gttacaagta actttacgtt gtttctcgtt tttcgatctt tgcaagccct cggaacagca
    11881 gctattccat taattgcagc tgctacaata acggattttt ttgatgggga agaacgaggt
    11941 agtgctatgg gaacttatca aaccttgtta tctattgctc ctgcagtagc tcctatttta
    12001 ggaggattta taggagaccg atacaattat gatggtgttt tctggtcatt agtgggtata
    12061 tctattcttt tacttattat caatatgttg ttttttccta aagaccagaa aaagcacaag
    12121 gcaagcaaga ggaacggtct tcatttttac cttgtaatgg cgaaggataa gatgggaagt
    12181 gtattgttgt cattaagctt cttgaacttt ttgatttact tttctatcct ggtttatctc
    12241 cccgttttat taacagacca ctatcatcta aacctaaaac ttgttggctt gctttatctt
    12301 cctatgacgc taagccttat agccggaagt ctgttattca aatacattca gaagaagatt
    12361 tctcttataa atatatttac ctttactaat tttttaatcg tatttagtgt tataggattt
    12421 ggactcacac atactgtttc tttactactt atgtcgctaa gcctattcat ttatggggtg
    12481 ggaattggaa tcttaacacc ttcctatgca accatattaa cccatcaatt tgaacaaaat
    12541 cgagctagtg ctatgggggt atttaacttt gttagatata ctggtatgtc tataggaccc
    12601 attttgtcag gtacattact agaatttttg agaagtgaaa ccatgtttat aatgttcggt
    12661 gtcatgtttg gcattataag tatctttggt tatttactag tggacaaccc caaaaacaca
    12721 ggtgaaaata gaactaccaa taaaccatct ttttaaacaa aaacatccaa ttaataacat
    12781 atttagaatt aagtattgac atttacaaat aatataaatt ataattctat ttattggaaa
    12841 tgaacatgtt gagcgttgaa aaggacacga attattttta tatttcttta cagagaatag
    12901 ggcctttggc tgtgagccct taagaaagag aaataattta ccaccttgga gctattgtgg
    12961 ggaaatatgt agatagagta acctcaatcg ggaaatccgt tatctttttg agaatataac
    13021 acttttagtg ttttataaaa ttagggtggt accacgaccg cactcgtccc tgttagaggg
    13081 atgagtgcgg ttttttatgt tcaaaaataa atagataagc aacgaaaagg acatgaatta
    13141 tttttatact tctttgcaga gaatagggtc tttggctgtg agcccttaag aaagagaaat
    13201 aatttaccac cttggagctt tattagtgaa ctagtagtaa ttaataacgt ttgcgacgtt
    13261 acagcaccag agccatcaat atatttttat attgatggga atttgggtgg taccacgtgt
    13321 gaaaacatac tcgtccctta cttagggacg agtatgtttt ttttgacttt atttaaatag
    13381 gagaggttat catgagtaaa gatttaattc aaattcgctt tttagatgga aatgagaaag
    13441 aattttcaaa aggtattaca ttacatgcta ttgcagaatc cattagttct agcctaaaga
    13501 agaaagctat tgcgggtaaa gtaaatggag aattgtatga tttgcatcga gccattaatc
    13561 aaaatgcaga aatcgaaatt ttaacagtag attctgacga aggtctagag attatgaggc
    13621 atacaacagc tcacatactt gctcaggcag taaaaagagt acataagaat gtgaagttag
    13681 gggtaggacc agttattgaa gatggatttt attacgatat ggatcttttt acaacgattg
    13741 cttctaatga gttattacaa ctcgaacatg aaatgaagaa aattattaat gaaaatcatc
    13801 ctattgaacg tactgaagtg acacgtagtg aagctgaaag aatatttagc gagataaacg
    13861 acgagttgaa actggagtta ttacgtgata ttcctatgaa tgaaacagtt acgatttata
    13921 aacaagggga atttttcgat ttatgtcgag gcccacatct tccttctact ggaatggtta
    13981 aggcgtttaa attaacaaaa gtatcaggag catactggag aggtaacagc gacaaccaaa
    14041 tgttacaacg tgtatatggc gtagcttttc gttctcaaaa acagttaaat gagtatctga
    14101 tgtttctaga agaagcagcg aaaagagacc atagaaaatt gggaaatgaa ctagaattat
    14161 ttatgttttc tgatgaagca cctggaatgc ctttttatct agctaaagga caaatggtta
    14221 aaaatcagct ggagcaattc ctacgatccg ttcaagaaaa gcataattac gaagaagtac
    14281 gtactccttt tatgatgaat caacaattat gggaaaagtc tggtcattgg gatcattata
    14341 aggataatat gtatttttca gaagtggata acgctaaatt tgcattaaag cccatgaact
    14401 gtcctggtca tatgcttatc tttaaaaata agctccattc ctatagagat ttaccaattc
    14461 gtatggggga atttggtcaa gttcaccgtc atgaattcag tggggctcta aatggattac
    14521 taagagtacg tacgttttgt caagatgatg ctcatatctt tgttactcct gaacaaattg
    14581 aaagtgaaat taaatcggtc attgaattag tggattatat ctacaagaca tttggttttg
    14641 aatatagtat tgaactttca actcgaccag aagactccat gggagaggat agcttatggg
    14701 aacttgctga aaactcctta gaaaacgtat taagaaagtt gaactttgat tataaattga
    14761 acgaaggcga cggagcattt tatggtccta aaatcgattt tcatattaaa gatgccttaa
    14821 aaagaagtca tcaatgtgca acgattcaat tagacttcca gctaccagaa aaatttgatt
    14881 tgaattatat taacgaaaag aacgaaaaac agcgtccagt tgtcattcat cgtgctattt
    14941 ttggatccct tgaccgtttc tttggcattt taattgagca ctttggaggc gtcttcccaa
    15001 catggttggc acctgtacaa gtaaaggtta ttccagtttc cctaaaggtt catcaggaat
    15061 atgcccaaga gattaaagaa gctttaggaa agaaagggat tcgagtagaa ttagataatc
    15121 gagaacaaaa gctcggatat aagattcgag aagctcaagt ggcgaaagtt ccttatattt
    15181 tggtattagg agataaggaa atggaaagca actccgtaaa cgtacgaaaa cacggtgaag
    15241 aaaagtctga aacgttcaat ttagatagct ttattgaaaa acttcaggat gaaattgttg
    15301 aaaagaaact tagataatta aacaaaaatg gttccttaat atttaataag ggaccttttt
    15361 gtatttctag tttactcttt cctataatga atcagcacag gtttttatat tttattatta
    15421 ggattttgta tactcatttt acaatttttt catataaaat cattgacttt attagtacct
    15481 agtactaata tcaatttata tatcttggta attaagaagg gtgaatacaa tgaaatcaac
    15541 tgcacgtatt acagcaatag gtacatatgt accagaaaaa agacttacca atgacgattt
    15601 agaacgaatt gtagatacga atgatgaatg gattgtgaaa cgaactggca tgaaggaaag
    15661 aagaattgcc gatgaaaacg aatattcttc tgacctagcc attaaagcaa ttgaaaactt
    15721 agtagagcaa tatcagaaag agttaaagga tgtaggatgt atcgtagtct caactacaac
    15781 acccgactat gcatttccaa gcgtgggagc ccaaatacaa gattactttg atattcctac
    15841 aactggggcg ctagacttta acgctacctg tgctggcttt acatacggat tacacatggc
    15901 aaacgccttt attacatctg gacttcatga taaagtctta gtcgtaactg cagaaacatt
    15961 atcaaaggtt acagattata cagatcgcac tacctgtgtt ctatttgggg atggagctgc
    16021 agctgttttg gtcgaaaatg atgcagagcg cccaagtttt attgctacgc atattggtac
    16081 aaatggaaag ggaggtaaac atgtataccg caccaattta tctactacca tgcatggaca
    16141 acctttgagt tcgagtggca agatggtgca aaacggaagg gaagtatata agtgggcatc
    16201 tcgaacaatt cctttaggta tggctgaatt gctagaacaa gcaaatctac aaaagagtga
    16261 agtaaattgg ttcgttccac acagtgccaa tttacgaatg gttgagtcta tatgtgaaag
    16321 atcagagttt ccagttgaga agacattaac tagtgtcgag tacatgggaa acacttcttc
    16381 cgtttccatt cctctagcac tacaaatagg gataaacgaa ggaaaattaa aagatggaga
    16441 cgttctctta ctctacggat ttggaggagg ccttacccac gcaggtcata ttattaaatg
    16501 ggatttaggt aaggagtaaa caattaattt atatcatttc aacaagagtt ttatagctaa
    16561 tgagaagtta gtaagtttgt atacacaatg ttagttaacc cttaaataac ctaaacaaag
    16621 atgaccggca tgacaataca aaaagatcct acatcaaaat aatgtaggat cttttcattt
    16681 acttttataa atagagtatg tttataaaag tatagaaaag cacccttact ccaagataac
    16741 atgtgtgtat acattattta tccctggtaa catggaactt tcaataaaag tgctttttca
    16801 acatgctctg gaatataact gcttaacctt cccccattag aatagatttc ctttaccata
    16861 cttgaactaa gaaaagaata tttttcttct gagtgaataa agaatgtttc agcttcttta
    16921 tatagatttt tatttgttag cgccatctgg gcttcatatc cataatcagt cacggttctc
    16981 aagcctttga taatcgcttt ataattgtgt tctttcatat aatctacaag taatccttca
    17041 aatgtatcaa ctttgacttt tttattatct ttaaatgatt gttcaattag ctgtcttctc
    17101 tcttcggtag aaaataaata ttgtttctta gaattcacca aaccagcgac taaaatttca
    17161 tcaaaaaatt cactagctct atttataata tcaatatgcc catttgtaat tggatcaaag
    17221 cttcctggat atatagcctt catttaatca cctcacttct tactactaac tattccgaaa
    17281 agcatttggt aatcttttca ttttgttagc aataataatt actttaacat cttgtaatag
    17341 atttgaatat cttgtacgta aaagtatgac tttttgaaca tcactaacaa aggaagactt
    17401 ccatttcgaa ttctttctat tttatatcgt tatcttaaat gttttaacaa agtcaataca
    17461 acttttcgaa atattgtttt attgaaggaa agcttccttt tggagcacct aatagttttt
    17521 cgtaaatctc gtcaatcgca tcaagtttgg caatgagttg ttcgtgagtc aatgagaaaa
    17581 ggctgggatc aagcatgaaa tggagtccga ctagtaagaa ctccgtaatt tcatctggaa
    17641 aattcgtgtg aaatacacct tcttccactc cttgcttcac aacagatgaa ataattggtg
    17701 catactcttt aatcatgcca actttcattt tctgatgtag aaggacgtta tctttgtgat
    17761 gcaaatgagt cattaatcca tcactgaaat gatttgatgt ttcctgtgaa aaaaattgaa
    17821 tgatgcttat aaacttttct attgccttta aatcagggtt attaataatt tgtttaaata
    17881 actggacact agaatcaata gagtcttgaa ttactgcgtc tgcaatttca tcttttgact
    17941 tgaaataatg ataaaaagta cctttcgcaa ctccaacttt tttaataata tcgttaaccg
    18001 tagtattctc atatccatta gatgaaaata aaccttttgc tacggtaata atctcgattc
    18061 ttctttcttc tggttttttg acgattcttt tcatggaatt tcccccttga gacatattat
    18121 tgtcttttaa agatacatca ttgaccgtcg gtcggtcaat agtgtatcat ttatttactt
    18181 aacatctgta gggagcgtga aaagatgaca aatggaaacc aagagcgaaa gtatgaaaat
    18241 gagcgcattc gtattgtttt aagagaaatt gataaaaaag tggttaaatt ggattatgat
    18301 tctggtggat taaaaaaaga tgtcattcat ttaaagaaaa ccttttggga tgatgtgaaa
    18361 attaatctag ataatcctga agatgctggt gaaaccttat caagcattag acagcaggca
    18421 gagatgctgt ccgagaggga aagaacgcac cgtcaactag ataagcagag gaagacctta
    18481 tctcgattaa aagattctcc atatttcgcc cgagttgatt ttcttgaaaa tggggaggag
    18541 acaacaaata acgtatacct cggagttgcg agtttaatga atgaaaaaga ggaaaaattt
    18601 cttatttatg attggagagc tcctatttcc agtatttatt atgattactc tccgggacca
    18661 gcccaatacg aaacagtaga agggatgatt caaggagaaa tgcagttaaa gcgtcagttt
    18721 atcacgaaag acggccaaat caaaacgatg tttgatacgg gtgtaaccat tggagatgaa
    18781 ctattgaaag aaatacttgg caataacgcg agtatcaata tgaaaagcat tgttgctacc
    18841 attcagaaag agcaaaatca gatcattcgt aacgtgaaaa gtaagtattt agttgtacaa
    18901 ggggttgctg gtagtgggaa aacctctgca gctcttcaaa gagttgcgta tttgttgtat
    18961 cgctaccaag atgagatcac ggccgaaaat attatgcttt tttcacctaa ttctttattc
    19021 aatcgctaca ttgcttctgt cctccctgat ttaggagagg acaatatgca gcagcttaca
    19081 ttccaagaat atgtagagaa gcgtcttgga aaacaatttg atgttgaaca tccgtttacg
    19141 caaatggaat atatgcttac atgtcaggaa cataaaatgt attctgccag aagaaaagga
    19201 atacggtata aagcgagtaa cgattttaaa tctttgattg actcctatgt tgttgttctc
    19261 tcaagtgaag actaccttat ctttaaaaat atttctttcc ggggagaaat gttgattgga
    19321 gctgaacaaa ttaaaagcta tttttattca ctagacgcat ctatctctat tccaaatcgt
    19381 atgcagttag taaaggaatg gcttctaaaa gagttagtaa agaaagaaaa attggagaga
    19441 accaaagatt gggtaataga agagagtgat ctgctagatc ttgaagagta tacaaaagtt
    19501 tatagtgagc tcctaaagga aggaagattt tctgaaaata cgttcgacga ttttgaacag
    19561 gagcaaaaga aaatagctaa aaagattgtc aaaaaacact ttaaaccaat acgaacagct
    19621 gtaaagaaac taaaatttat taatgtggca gcgctttaca gccaattgtt taagcagaaa
    19681 gataacatct accaaaagct taatgataca tttcctgaag gttgggaaga aatttgttca
    19741 tatacgttaa aaaagctaca tagaaaagaa ctgacgtatg aagatgcagc tccttatttg
    19801 tatctccaag ataaacttga ggggaaaaga ataaacacaa gaatacgtca tttatttatt
    19861 gatgaagcac aggattattc accctttcag ttttttgtgc ttaagcagct ttttccttat
    19921 tgccgaatga cgattctagg tgattttaat caggcgattt atgcccatac tctaaatgca
    19981 ccaaccttac tatcaaacga attgtatgta aaagaaaatt ttgaaaaagt ggtgttaacg
    20041 aaaagctacc gttcaactcg tcaaatcgtt gaatttacaa agggaattat agaagaagga
    20101 gaaactattg aagcctttaa cagaaatggt cagaagccta cttttacgaa ggtagaggac
    20161 ctggacaccc tccaatctcg aataacaaag aagattaggc aactgcagtc agcaaaccat
    20221 gaaacaattg ctgtgatatg taaaacagga aaagaaagct gggaagccta tgaccgattg
    20281 agtgagcaaa taaatatcca gctgatggaa cataatacga gttcttatcg aaaaggtatc
    20341 ttaattttac ctgcttattt ggcaaaggga atcgaatttg atgctgtgat aatatacgat
    20401 gcttctgatg taacatatgg aagagaattt gaaaggaagc ttttttatac tgcatgtacg
    20461 agggccatgc acgagcttca tcttttctcc gtaggggaga tgagtccgtt tatgaataag
    20521 attcctgcaa attcatatga gattacggag taagtataac ttcaatgagg aaaaatggcc
    20581 ttccaccact ttaattcctg acagggttcg cggaagcttt tttctgtggc attaaatata
    20641 aaagccgatg attccttagc gtagggaaaa tcggctttat tcatatcgaa atttgcttag
    20701 cgtatgtttt tcgc
//
