LOCUS       NZ_CP064275            28751 bp    DNA     circular CON 17-APR-2024
DEFINITION  Priestia megaterium strain B1 chromosome, complete genome.
ACCESSION   NZ_CP064275
VERSION     NZ_CP064275.1
KEYWORDS    RefSeq.
SOURCE      Priestia megaterium (Bacillus megaterium)
  ORGANISM  Priestia megaterium
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 5078588)
  AUTHORS   Wang,C., Jia,Z., Zhao,J., Wang,X., Liu,Z. and Ji,F.
  TITLE     Comparative Genome Analysis of key enzyme genes in ammonia nitrogen
            Metabolism Pathway of bacteria from different sources
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5078588)
  AUTHORS   Wang,C., Jia,Z., Zhao,J., Wang,X., Liu,Z. and Ji,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2020) Guangdong Key Laboratory of Animal
            Conservation and Resource Utilization, Guangdong Public Laboratory
            of Wild Animal Conservation and Utilization, Institute of Zoology,
            Guangdong Academy of Science, Microbial Pathogenic Ecology Group,
            105 Xingang West Road, Guangzhou 510260, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. V1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 252.67x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022811885.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 10:12:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,571
            CDSs (total)                      :: 5,383
            Genes (coding)                    :: 5,338
            CDSs (with protein)               :: 5,338
            Genes (RNA)                       :: 188
            rRNAs                             :: 15, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 137
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 12 of 46
            Pseudo Genes (incomplete)         :: 26 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:16:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3952154
            Orig. end                         :: 3980905
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP064275.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..28751
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="8"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..28751
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..28751
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:18751](+)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..18751
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     gene            247..957
                     /locus_tag="IQ966_RS27970"
     CDS             247..957
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /locus_tag="IQ966_RS27970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycosyltransferase"
                     /protein_id="WP_341715527.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 3.5e-33,
                     bitscore: 112.9, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDPKFDIRYFRKLNNIITVSEECGNILKSRFPSERNKVRIMYNIV
                     SPKLINKMAKQVKMGVYDKKCNEIVILSIGRLHYQKGFELAIKACRKLVDKGFNIKWNI
                     IGDGEERENLTKLIKINNLENHVILLGLKSNPYPYIKQADIYAQTSRFEGKSIAIDEAK
                     ILQKPIIVTNFSTARDQITDGVNGLIVDMNSIAISEGIEQLIKDFELKKTIISNLSKEE
                     LGTEEEINKLYEIL"
     gene            971..2156
                     /locus_tag="IQ966_RS20480"
                     /pseudo=""
     CDS             971..2156
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016132435.1"
                     /locus_tag="IQ966_RS20480"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="glycosyltransferase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKKNLLFVMNNLSCGGAEKALISLLETIDYSKFNVDLFLFKHEGL
                     FFRKYLNK"
     gene            2146..3354
                     /locus_tag="IQ966_RS20485"
     CDS             2146..3354
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002191261.1"
                     /locus_tag="IQ966_RS20485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="WP_243549234.1"
                     /transl_table=11
                     /translation="MQDRKGHRNDYSNKCFLLITLIVIVILIDIIPIFKDWLGRIHIGR
                     YDNKNIWNKLITEKAVSWLNDTPKIKVTDNTRLIVLDMLKGNYTKSAIQHWQEASILLG
                     LSEYLKSIEDHKAENEIVKFLNKKFDSAGQWIKKPVHVDGAILAYAVMKLDFIETDTYR
                     KALDYTWEMIKEHIGEEGTVEYRKSMPSYRYVDTIGFICPFLIAYGTKYNKGECIELAV
                     KQIKEYERYGMLEIYSIPCHAYKVDNKIPLGLYGWGRGLGWYAIGLIDSWAELPTDSRH
                     KSILEESVKKFAKAAMSFQQDNGSWNWTVTRNESRADSSTTATLGWFLINASKIDGISK
                     ECLDSTTKAINYLMSVTRRTGEVDFSQGDTKDIGVYAMLFNILPFTQGFCIRMINAISI
                     TKK"
     gene            3791..5349
                     /locus_tag="IQ966_RS20490"
                     /pseudo=""
     CDS             3791..5349
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002160849.1"
                     /locus_tag="IQ966_RS20490"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /sec_met_domain="Polysacc_synt (E-value: 9.1e-11, bitscore:
                     40.0, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTKHSIINIATGLGNQIIITSLSFISRTVFITYLGVEYLGISGL
                     FTSILAMLALAEAGVGSSIVYSLYKPVAENDQEKINVLMNLYKKAYMVIALIVLLVGLS
                     IMPFLELFTKDTSVENIHLIYLIFLINTVLPYLYLHKNNFLSVCQKTYMITGIYSISSI
                     ASVCLKIGILYYTKNFILYLVADCFITIVTSAVLAIIVNKMYPFLKNKVTRKLDSETRS
                     NIIKNIKAIVLQNIGTYLVFGTDNIIISTFVSVTAVGLYSNYNMLIEICRTFVNQVFSN
                     IYHSVGNLIAQETKEKVYSIYKVTMLLSFWLYSTLGILLLIMLEPFIAIWIGSKFLMSK
                     GVVIILIIIFYERGMRNSISTVKTTSGIFHEDRFAPLFQAAVNLVISIIVVQHIGIAGV
                     FIGTLISALVVPFWVTPYLVYKKVFNKPIIMYFVRYFYFTIIATGTYFTTNLVWSLIEP
                     DTFVTLVLKGIICLITINTIYILVFTKPRSSNIYLI"
     gene            complement(5649..9623)
                     /locus_tag="IQ966_RS20495"
     CDS             complement(5649..9623)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /locus_tag="IQ966_RS20495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="WP_208732357.1"
                     /transl_table=11
                     /translation="MAYKIQISSSLKKDLPILGIGEFGLCTDTAELFIGSDSGNIQIAN
                     KSEIGLLSNLTTIDKENLVNAINEIERKISDNHGATSLDELSDVDISTLSPKNGDVLKY
                     EAGIWKPATNLSGTISSSPYVIELNRWNIKNDGTSSKTTTQGINDAIIWAKSQGVNHVI
                     LPSGFYKLKLKPWVWQDAASLSFTCINMVNDLHFEMADGCILELEGNSSPGYSIFQVKD
                     VKNVKISGGKIIGDRKTHYYEIGVKFVRGGINPDGSLNDNLNFIRSEIIDRFKDPGLLN
                     NFRLWKIEGIDVSKGYSFYQYKDTVSNNTLVGFRNNGQFAPSSPTGRGWLLQPNIGANN
                     KMVFTIDITNTPLTDNQIAAITAKVDNERYTHEWGQGIEIAGSNHVEIKNTEICDCTGD
                     AVSTGWLQYRTNSSDYTQNEMGHHIWVHGCDIHHCRRQGITLASANDVYIYDNKIHHIG
                     KADDNVTSDFRNGIPPMFAIDIESMVGESNIPYKQPYYNKDGLELNFRLYIFNNHIHDN
                     ERGHFVNADGNHVTVENNIFEGWNVGGVSSYPTNMYIKYLNNTFINTEFIVSGDNYVNG
                     LVGQQSNLRLMDVRGSVIQDIRLNNGIFYGNANYGYFGTPTVDVSTSSFIINNHGMGNT
                     AKICFEQWVGKVPSSISVDKIYYVVNRTTNSFQVSETEDGPPVTFYDSGEWGFNVSRFN
                     YGRCYINNVVIERDWNSGGTTPGFAVTAAGSIIQNVTVKNHSVSINGNGSNIYVGRPLT
                     VDGLTIIDGSANIKDCNVLNSQFIRNRNKSISPDLKFGDSAFPETKVNIKNSLFRNIDA
                     TLINSTVDNCSFFNVKLSKTDNNTQTILNHCYLENSIVDGYWLELKKGYLITRCTLLNT
                     AMTVNKNPGTTLLLENIDITNSTADLNAPRVTVNPNGGIYNTDKTVTLLANEDAIIYYT
                     LDGTLPTTSSIQYSTPLKLSNPTTLKVIAVDAAGNQSKVKSVQFNIDKTPPTNVTNLTA
                     APFNDKVDLSWTASTSNDVLNYEVYNGDTLIRSINSTTYTVNGLLAASQYSFTVKTVDK
                     AGNKSSGISIIVTTSQDTTPPEEISNLLASSITSDSIGLTWTRSLSSDVASYEIYQDSV
                     LLNGNVTTTNYSITGLKANIQYKLTVKSKDINGNVSDGISVTVETSAVSANYVTNGLIL
                     YKDNPSNNLIIENPDSYFMVNDTFTVSFTAKVPKFGDLISRYELGGSQNKILLERNGNN
                     AFGFSIWGNTANTTVTSPQYADESLYYHIDVIRTGTALLMYINNVKVSEAGIANNVYTK
                     PSSLPLIIGDKAVPVLFKNVAYYNRILTPEELALNFNALK"
     gene            10001..11941
                     /locus_tag="IQ966_RS20500"
     CDS             10001..11941
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GATase_7"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378582.1"
                     /locus_tag="IQ966_RS20500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="asparagine synthase-related protein"
                     /protein_id="WP_208732356.1"
                     /sec_met_domain="GATase_7 (E-value: 5.8e-21, bitscore:
                     72.9, seeds: 307, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 7.9e-22, bitscore:
                     76.6, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAIAGIYHSNNEPVPVEHVNRVMQSLQKYPANDIQISHKKNIFL
                     GCHAQWITPESVGEQQPYCDYERQLAITADVIIDNRDELFEKLQVKYIERKAMTDSQLI
                     LLAYYKWGEESPKYLVGDFAFVIWDERKQILFGARDFSGSRTLYFYHDTNRFAFCTTIE
                     PLLSLPYVEKKLNEQWLAEFLAISGMVDVVDASLTPYKNIAQMPPAHSITVMEETVKVK
                     QYCTLTSVQTLKLKSNEEYIEAFQEVFQEAVTSRLRTYRQVGAQLSGGLDSGSIVSFAA
                     KSLSGTRKQLYTFSYVPPNDFKDFTPKHLMPNETPFIQSTVQHIGGIKDHYLDFEGRDS
                     YSEIDDFLNTMEMPYKFFENSFWLKGMFEKASEENVGVLLNGGRGNLSISWGSAIDYYA
                     LLLKKLKWIRLASELHHYSHKAGGSRFRRIPVIARVAFPFIERLFPLGDSYEFPMLINP
                     EFAKRTGVFHKLQNYGIDETGWFSATNIYEQRKRHFEDIFHWNASNTLATKLSLRYPLW
                     KRDPTNDLRVIRFCLSLPEEQYVQKGMDRALIRRATEKLLPDTVRLNQRIRGVQGADWV
                     HRMMPHWSEFTDELHQLSKDQTILEFLDGKGIKEAMSNIHEGVHAKHAINPYYKIMMRS
                     LIVHRFIKKFN"
     gene            11956..12111
                     /locus_tag="IQ966_RS20505"
     CDS             11956..12111
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002160858.1"
                     /locus_tag="IQ966_RS20505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="paeninodin family lasso peptide"
                     /protein_id="WP_108674682.1"
                     /transl_table=11
                     /translation="MKKEWQIPVLETLDITMTMASTVYGPYTDEAYVPGKDVDKDNPGT
                     WKRFTS"
     CDS_motif       11956..12051
                     /prepeptide="leader"
                     /note="peptide class: lassopeptide"
                     /note="predicted sequence:
                     MKKEWQIPVLETLDITMTMASTVYGPYTDEAY"
                     /locus_tag="IQ966_RS20505_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       12052..12111
                     /prepeptide="core"
                     /locus_tag="IQ966_RS20505_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="-12.10"
                     /molecular_weight="2129.3"
                     /monoisotopic_mass="2128.1"
                     /core_sequence="VPGKDVDKDNPGTWKRFTS"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MKKEWQIPVLETLDITMTMASTVYGPYTDEAY"
                     /leader_location="[11955:12051](+)"
     gene            12579..13511
                     /locus_tag="IQ966_RS20510"
     CDS             12579..13511
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378579.1"
                     /locus_tag="IQ966_RS20510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aldolase"
                     /protein_id="WP_048021074.1"
                     /transl_table=11
                     /translation="MIDTTKKVVYEAFGLKLLSEIPLPELPEISISGGSVEIIVKKGDL
                     TTLWSEKNKSGAYFIVKENLCMFKVPGLAIYLIQDGKEITVSPTEGFNEDLSRLYILGT
                     CMGALLIQRKIFPLHGSAIAIDGKAYAVVGESGAGKSTLASAFLKRGYRLLSDDVIPVT
                     FTEENIPIVTPAYPQQKLWQESLTQLGMESTCYRPIFDRETKFTIPVESQFATEAYPLA
                     GIFELIKNDSNEIKIQSIEKLERLHMLFNHTYRNFFIELSGLMKWHFDASAKIVNKVDL
                     YRLHRPISGFTAYDLTDLILTTIKRRENK"
     gene            13508..13813
                     /locus_tag="IQ966_RS20515"
     CDS             13508..13813
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thurincin_rSAM_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378578.1"
                     /locus_tag="IQ966_RS20515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_048021072.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 6.4e-27,
                     bitscore: 91.7, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 3.8e-16, bitscore: 57.4,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="Thurincin_rSAM_RRE (E-value: 9.1e-07,
                     bitscore: 27.1, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKNQDISMNQLVSQETGHIVSDMDGEKVMLSISNGKYYNLGEIG
                     GEIWEKIKEPISVSELISTLVTQYNVEQSDCEEQVIAFLSGLIKEGLIQVAENNRS"
     aSDomain        13550..13792
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_IQ966_RS20515_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="3.50E-29"
                     /identifier="RREFam002.1"
                     /label="IQ966_RS20515"
                     /locus_tag="IQ966_RS20515"
                     /protein_end="95"
                     /protein_start="14"
                     /score="91.7"
                     /tool="antismash"
                     /translation="QETGHIVSDMDGEKVMLSISNGKYYNLGEIGGEIWEKIKEPISVS
                     ELISTLVTQYNVEQSDCEEQVIAFLSGLIKEGLIQV"
     aSDomain        13601..13780
                     /aSDomain="Thurincin_rSAM_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing rSAM protein in a thurincin
                     sactipeptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_IQ966_RS20515_Thurincin_rSAM_RRE.1"
                     /evalue="4.90E-09"
                     /identifier="RREFam019.1"
                     /label="IQ966_RS20515"
                     /locus_tag="IQ966_RS20515"
                     /protein_end="91"
                     /protein_start="31"
                     /score="27.1"
                     /tool="antismash"
                     /translation="SISNGKYYNLGEIGGEIWEKIKEPISVSELISTLVTQYNVEQSDC
                     EEQVIAFLSGLIKEG"
     gene            13846..14328
                     /locus_tag="IQ966_RS20520"
     CDS             13846..14328
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378577.1"
                     /locus_tag="IQ966_RS20520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_048021070.1"
                     /sec_met_domain="PF13471 (E-value: 9.3e-24, bitscore: 81.9,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKILRKITVFLKLNMRAKLLLIEAFIYLGWARFLKTLPFSKISPS
                     LGQQMKETAFTTSSSDDKVLKDIAQSLHIMSRYTFWESQCLVKAIAGMKMLEKRKIEST
                     LYLGTAKDENGKLIAHAWLRSGPFFISGAEGKAKFTVIAKFAKEISVKKREGENYG"
     gene            14321..15514
                     /locus_tag="IQ966_RS20525"
     CDS             14321..15514
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_5"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002160862.1"
                     /locus_tag="IQ966_RS20525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nucleotidyltransferase family protein"
                     /protein_id="WP_108674683.1"
                     /sec_met_domain="NTP_transf_5 (E-value: 8.5e-58, bitscore:
                     194.0, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDNILNLDRMPKELKLILELLKEGNEQYLEKYSEGLCRDIDWNLF
                     IKLTLHHRVYPAINFKIKMLDADVVPTYVVQFLNRQYKRNTFQMLRLSAEMEKVSLLFS
                     KHQIQTLFLKGPILAKCLYGDISLRTSSDLDFLIPIQDLAKAEDLLVSLGYEKDDYIHT
                     VLNDWKWRHHHVTYIHPHTEIKLEIHWRLHPGPGKEPCFDELWKRKEKSSITSYPVYLL
                     GTEDLFQFLVAHGARHGWSRLRWLIDIQQIVKRKIDWVKLYTLFTTYHNPYVGGQAIIL
                     ASQLLNVRVEKKMKPLLISKRSQKLAQEAVFYLETMINLHTDSVPEYVSKYHSRHLFSL
                     MSAQQKLFFTASLLHPYPEDAEMLPLPKKLHFLYFPLRPVLWAWRKTKKDELRKGRT"
     gene            15514..17307
                     /locus_tag="IQ966_RS20530"
     CDS             15514..17307
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020061976.1"
                     /locus_tag="IQ966_RS20530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_048021066.1"
                     /transl_table=11
                     /translation="MKQLVYFLKQIHSFAGKRLYINLLATVFMSLLEGIGILLLIPMIS
                     LSGIVDFDTGGPSILRVFSFLQNTPVSMTLLLILSIYVLIVTGQTILQRQITIQNRVIQ
                     LGFLRHMQMETYSTLLHANWEFFIKKRKSDLINIVLTEIARTSGGTTSFLQFIASLIFT
                     CIQIGLAFWLSPKITLFVLLCGVVIIFLNRKFLKRSLTLGNRNFELGKSYLAGITDQIN
                     GIKDIKSNTLEESRMNWYRSITEEMKNEQVEYTKLNTTSQSYYKISSAVLVAAFIYAAV
                     KLFHAEASELMLIILIFSRLWPKVAGIQASMEQISTTLPAFKAVKALQDECKNAREFEK
                     EANQSVEPVQFKKDIQCRNVYFRYNQGASYALQHINLIIPSNQMTAFVGRSGAGKSTLI
                     DLLMGLNQPEKGQVLIDGIPLSKDNLLSLRRAISYVPQDPFLFNASIRENLLLVEPNAS
                     EQRMWEALEFSSAAEFVRRLPQGLDSLIGDRGIKLSGGERQRLVLARAILRNPSILVLD
                     EATSALDTENEAKIQEALERLKGKMTIIVIAHRLSTIRNADQVIVLEQGKIIQQGGFTQ
                     LAEEKSNTFSQLLGKQMEAAH"
     gene            17387..17590
                     /locus_tag="IQ966_RS20535"
     CDS             17387..17590
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IQ966_RS20535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_048021064.1"
                     /transl_table=11
                     /translation="MQEKTYSAPEVLNHQLIRFETAQSWNPGKGNLDHPGKGNNGSNWP
                     STNPNPTPGGAGAPGKGKGKNK"
     gene            17700..18485
                     /locus_tag="IQ966_RS20540"
     CDS             17700..18485
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383222.1"
                     /locus_tag="IQ966_RS20540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_048021062.1"
                     /transl_table=11
                     /translation="MKTLHTKIGDHYIDIKTEFDDVWKIIKQYFLCVSIAKNQPDMTVT
                     ITNGYGVSFQDYEVAIEEKLNTITFKRADYLIEVDSEYKLANVAIHDELALKHALMNLY
                     SSFIVHHNWGILLHSSCVINSGKAHIFTGHSGAGKSTAAKLSMPRELLSDEATILRITD
                     ESVTVFNSPFRSELKAKGSEKNTPLESIQLLHQSISNQRIEVRRIEALMKLIDKVFYWS
                     YKPEETKTIMGLLQLLVKQVPVYELHFQKNNTFWELMSS"
     gene            18482..18751
                     /locus_tag="IQ966_RS20545"
     CDS             18482..18751
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PqqD_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /locus_tag="IQ966_RS20545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PqqD family protein"
                     /protein_id="WP_115653802.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 5.4e-16,
                     bitscore: 56.7, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PqqD_RRE (E-value: 5.2e-07, bitscore:
                     27.6, seeds: 9, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 1.2e-15, bitscore: 55.8,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSYIQKGGYEVMEVDSEWVILNSHAYTVTTLNEVGGYCWKVIEN
                     KKTLAEIIKAVAAYYNTEAEMVEGDIQHFLNKLIDCGLVEKYAS"
     aSDomain        18500..18739
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_IQ966_RS20545_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="2.90E-18"
                     /identifier="RREFam002.1"
                     /label="IQ966_RS20545"
                     /locus_tag="IQ966_RS20545"
                     /protein_end="86"
                     /protein_start="6"
                     /score="56.7"
                     /tool="antismash"
                     /translation="KGGYEVMEVDSEWVILNSHAYTVTTLNEVGGYCWKVIENKKTLAE
                     IIKAVAAYYNTEAEMVEGDIQHFLNKLIDCGLVEK"
     aSDomain        18524..18736
                     /aSDomain="PqqD_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing protein in a pyrroloquinoline
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_IQ966_RS20545_PqqD_RRE.1"
                     /evalue="2.80E-09"
                     /identifier="RREFam006.1"
                     /label="IQ966_RS20545"
                     /locus_tag="IQ966_RS20545"
                     /protein_end="85"
                     /protein_start="14"
                     /score="27.6"
                     /tool="antismash"
                     /translation="VDSEWVILNSHAYTVTTLNEVGGYCWKVIENKKTLAEIIKAVAAY
                     YNTEAEMVEGDIQHFLNKLIDCGLVE"
     gene            18741..19199
                     /locus_tag="IQ966_RS20550"
     CDS             18741..19199
                     /EC_number="3.4.21.89"
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /locus_tag="IQ966_RS20550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="signal peptidase I"
                     /protein_id="WP_048021487.1"
                     /transl_table=11
                     /translation="MQANNKQGHLLKALIDRNGLLELPAEGTSMYPLIKQGDICQFTSI
                     KHSPLKRGDIVLFESPANKLIAHRLLNCSYVQEEEYYIFKGDTNYGVDDLVTREKIIGK
                     LVVIQKKSVAIQEKNVIAVLWGKLILAFPLLSVLLRKYINYQEWKKAD"
     gene            19215..21026
                     /locus_tag="IQ966_RS20555"
     CDS             19215..21026
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="resistance (resist) ABC_efflux"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383225.1"
                     /locus_tag="IQ966_RS20555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_208732355.1"
                     /transl_table=11
                     /translation="MISLKKIKQFDIREYISIKETKSMVQWLWPYVMKHKGAYIGLFIL
                     LFVEITLTLVSAWFIGNITDAAVHGNFERLKWLVAIGVGLSVTSITANFIDTYLETIAV
                     NAVKRDLNLDLYKHILLLRERDVSNLHSGELLSHFTNDIHNVEGVIGRGLINLVRFPII
                     SIAAFIYLVQINWKLLLSIVLVSPIAIAGGAIFGILLRNNSRIMNELISNIIKKLNDTF
                     HGFTVIRSFTLEKLFYRDYRQKNEQLYSLEMREAKLRGWFYAGSEAIGAIAFLISLIIG
                     AFFVTDKIITIGALLTFINLVNQLISPLTGLAGQWAGYQRSVSALERITKLLNYPTQAK
                     ALPHYTSATLVQKDIQVRNLTFSYDESAIVFKDFNLYIPAGKAVALVGPSGAGKSTLFN
                     LIQHFYEPHFGTISIDGRSIDELTISELRSSIAYVPQETFLFSGTIKDNLMIARPLATE
                     EEMINAAVHANIHDHIMTLRNGYETEVGERGAKLSGGQRQRLAIARAILKDAPILLLDE
                     ATSALDSETEHLVKEALARLMINRTTIMIAHRLSTIQHADLIVVMDQGEVVQKGTHEEL
                     IRQSGMYRRLNNAQEISQQNPIMLKAK"
     gene            21045..22148
                     /locus_tag="IQ966_RS20560"
     CDS             21045..22148
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_5"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383226.1"
                     /locus_tag="IQ966_RS20560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nucleotidyltransferase family protein"
                     /protein_id="WP_080968661.1"
                     /sec_met_domain="NTP_transf_5 (E-value: 2.8e-38, bitscore:
                     130.1, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDKLITHLYDSNANLPSDDEFYYEVIKEIELYFISPQVYSLLKE
                     KGELGNTPLFFQTRLKQKYQETLYQNLFIKNQTDHIIRKFEEEQIEVIPLKGVYFAEKY
                     FGHIGARGTSDIDILVKRKDINKAIEVVKSMDFKIEEEFIPSHFHVSFSREIPGSAIPL
                     TVEIHWDILKEKTANFNIQEFWGEAVRLEGFQFVNALSHYHTFYMICLHGWRHNLDSPK
                     HFLDIIQMIDVVKDKISYETLFKDAKRHQTYKRMIRTLSIVYSQYSILEQIKELSSIEN
                     QFKGFASSTLEQEKKGIKKYFTFMDYQFFSYDSVWHSLSEMRSWLLPNRFEVTSQLKET
                     SKQHSYFYSLLMLYKQRCNSVVKVLWS"
     gene            22236..23162
                     /locus_tag="IQ966_RS20565"
     CDS             22236..23162
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /locus_tag="IQ966_RS20565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="metallophosphoesterase"
                     /protein_id="WP_230683513.1"
                     /transl_table=11
                     /translation="MKGKVIAASFFLIVVLIGYTVWDNQRITVAKQDIIIKHLPKELEG
                     FSIVQITDLHEKEFGENQERLINTINSIHYDAIVFTGDMMKNNSENAVPFYTLIEGIEN
                     KQHALFVHGNTDPKSYRTSSSNLEKGKFVKDMESKGVRLLDSTYTVKRGNAHIQFVNFD
                     WSLKTENKSKTSGSHLSNNKGYPEKETSDLVIGLNHFPVVDSHIDALLRDPSVTFRYYD
                     LIMAGHYHGGQFRIPFFGALFVPEAWYEKSGLFPPQDRVKGLWEYKHIKQYVSAGLGSN
                     GPIPFLNFRFFDSPEINVLTFKSRKTE"
     gene            complement(23265..24389)
                     /locus_tag="IQ966_RS20570"
     CDS             complement(23265..24389)
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010676616.1"
                     /locus_tag="IQ966_RS20570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_048021058.1"
                     /transl_table=11
                     /translation="MNYQTDVCIIGAGPGGALLAYLLAKQNISTILIERSDKLGKEFRG
                     EHLNEDGEMILKKHHIFEEIESLGLLRMSRVEYWKDGHVFKTIESELGHAGIHVPQQHL
                     LQSINRQASLYPSYKLMLGTKVTELMQNEKGQYTGIKAEQKGRGEIIVESKIIIGADGR
                     YSTVRKLAHIKNNIHNPGYDLLWAKIPAPEKWKPTIRFTVVNNQQMALFTQTGGFIQIG
                     WNIKKGSYTSLKKQSVQPFLDQLILAFPQLKESIEQNLHSWKNFILLSVYSSHCDTWVK
                     DGLAIIGDAAHTMTPTGAYGMNAAIMDADVLSQILPKLLSHGSLEAGQLKKFENARRKI
                     VEDLQDQQAMTEASFIENFSAILKIQEERLPLFQ"
     misc_feature    24666..24684
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 22.77"
                     /tool="antismash"
     gene            24721..25116
                     /locus_tag="IQ966_RS20575"
     CDS             24721..25116
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014460246.1"
                     /locus_tag="IQ966_RS20575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_014460246.1"
                     /transl_table=11
                     /translation="MTKVEHYIQTLGNSADLLTKKQTSIYFEKLSNTFPFLTIMQINWR
                     KVLIKKSARHIEEIKKWLQEMNIKEHQVVLFWKRATKAVSVDLAQALTFFQQTADLTEE
                     AFIYCPSVDYVIEYFKDGKMMIGLAAR"
     gene            25338..26114
                     /locus_tag="IQ966_RS20580"
     CDS             25338..26114
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013083025.1"
                     /locus_tag="IQ966_RS20580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_279400973.1"
                     /transl_table=11
                     /translation="MSNKKAVTNQTKKEYPKPYGYTSDIAVFTIVTEEKEPYKPPKMNL
                     KLMLIQRAMLNAEGEKNSEAGKWALPGGFVHPDESAFEAAKRELEEETGVNDIHLKHYG
                     VYDEPDRDDRGWVITNAHYAIVPEDSLIKRKANDDAARVELFKIDEIFSLPLAFDHEII
                     IRDAIKEIKKDLLQTTIAQKFLPSQFTYSELQAVLLTVTDDAAIKSDQAFARKIRMLPF
                     IEEVDGKTTTRTSKKPTKLYRFVEMNVMKPIYTARY"
     gene            26187..26735
                     /locus_tag="IQ966_RS20585"
     CDS             26187..26735
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027: isochorismatase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013056905.1"
                     /locus_tag="IQ966_RS20585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="WP_014460244.1"
                     /transl_table=11
                     /translation="MKKALINIDYTNDFVAENGALTCGEPGRHIETSINDITRQFIEQD
                     EYVVFAIDFHKENDSLHPESALFPPHNIEGSAGRKLYGKLNDLYNVYQSKANVEWMNKT
                     RYSAFAGTELEIKLRERQITDVYLAGVCTDICVLHTAVDAYNKGFAIYIYEKAVASFNQ
                     KGHEWALEHFKSCLGAKII"
     gene            26759..28228
                     /locus_tag="IQ966_RS20590"
     CDS             26759..28228
                     /EC_number="6.3.4.21"
                     /GO_function="GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014460243.1"
                     /locus_tag="IQ966_RS20590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="WP_014460243.1"
                     /transl_table=11
                     /translation="MNMFTDDSVMLHTDLYQINMGETYWEDNIHQKKAVFEVFFRKLPF
                     GNGYAVFAGLERIIEYIKAFKFSESDLTYLHEELGFKEDYLAYLKDLTFTGTIRCMREG
                     ELVFHNEPILRVEAPLIEAQLVETAILNIVNYQTLIATKAARIKHVVGDQVAMEFGTRR
                     AQEMDAAIWGTRAAYIGGFGATSNVRAGKKFGIPVAGTHAHAMVQAYKDEYIAFHKYAR
                     RHKNCVFLVDTYDTLKSGVPNAIKVAKELGDKINFTAIRLDSGDLTYLSKKARQMLDEA
                     GFTETKIVASNDLDEDTIMHLKSQGAQIDMWGIGTKLITAYDQPALGAVYKLVSIEGEN
                     GEMMDTIKISANPEKVSTPGLKRVYRIINNLNGKSEGDYITMEEEDPQSEERLKMFHPV
                     HTFISKFVTNFEAKELHHDIFVNGKLVYETEELQEIQQYVKDNLELLWDEYKRTRNPEE
                     YPVDLSEKCWENKTRQIAYVKQKVAEALNKK"
ORIGIN
        1 tactaaaagg taaaatatct ttactattta ataggatcat gtttgcatta ataaataaaa
       61 ataataaaaa catttctcaa aaagaacaat atggctggaa gtatatttct gaatctttag
      121 aaaaaattga taaaaatatg atgtagctat aggtttttag agaaaacatc tacctacttt
      181 tgtatagata aggtagacgc tttaaaaaaa ataggttgga ttcatattga ttatgacaaa
      241 ttaggtatgg atccaaaatt tgatatacga tattttagaa agctcaataa tattattacc
      301 gtctcagaag aatgtggtaa tattctgaaa agtcgtttcc ctagtgaacg caataaagta
      361 agaatcatgt acaatatcgt ttcaccaaaa ttgataaata agatggctaa acaagttaaa
      421 atgggtgttt atgacaaaaa atgtaatgag attgtcattt tgtctatagg aaggctacac
      481 tatcaaaaag gatttgagtt ggctattaaa gcatgtagaa aattagtaga taaaggtttt
      541 aatataaaat ggaacattat aggtgatggc gaagagagag agaatcttac aaagttaatt
      601 aaaataaata atctggaaaa tcatgttata ttgttaggat taaaatctaa cccatatccc
      661 tacataaaac aagccgatat atatgcacaa acatcaaggt ttgaaggtaa atctatagct
      721 atagatgaag ctaagatcct tcaaaaacct attattgtta ctaatttcag cacagctaga
      781 gaccaaataa cagacggtgt caatggacta atagtagata tgaattcaat agctatttct
      841 gaaggaattg aacaacttat aaaagatttt gaactcaaaa aaaccataat aagtaattta
      901 tctaaagaag aattaggtac agaagaagaa ataaataagt tgtatgaaat attatagagg
      961 gtgactatta atgaaaaaaa atctattatt tgtaatgaat aatttaagct gtgggggtgc
     1021 cgaaaaggct cttatttctt tattagaaac catagattac tctaagttta atgtggactt
     1081 atttttattt aaacatgaag gtttattttt tcgaaaatac ctaaacaagt aaatttatta
     1141 aaagagcctt atgaatataa atattatgac atgcctataa aagagcaata ataagttgta
     1201 ttaagaaagg cagacttaac atagctttat caaggttaaa agctggtttc atatttaaaa
     1261 ctgaacaaaa cagagttagg tgtgaacaaa gagtctggaa atatatatgt aattctttga
     1321 aaagtataaa taagaagtat gatgttgcaa ttggttacct ggaaaaaacc cccatttact
     1381 tttgtgtaga caaagtaaat gcaaaaaaga aaataggatt tattcataat gattacgaca
     1441 aacttggaat ggaccctgaa attgatcaaa aatatttcga taaactgggc aacattgtta
     1501 cagtatctga agaatgtgct agtgttttaa aacaacgatt tcctgtatac aaagacaaag
     1561 ttgaagtgat gtataatatt gtttcaccga aagtaataaa taaaatgtca tcgaaccatt
     1621 tgaacataaa ataccgtgga gtaaatttag tttcattagg caggttacat caacaaaaag
     1681 ggtttgagct ggcgatagaa gcatgtaaga atttaattga tagcggttat aaaataaagt
     1741 ggcatgtaat tggagaaggg gaggaaagaa ctaagctaga aaagctaatt aaagccaata
     1801 ggcttgaaga gaactttata ctgttaggtc ttaaagaaaa cccatatcca tacataaaaa
     1861 atgcagatat ttatgttcat ccttccagat ttgaaggtaa atctatagct attgatgaag
     1921 caaagatact taataaacca attgtagtaa ctaactttac tacagttaaa gatcaaatta
     1981 aagatcagga aactggatta attgtagata tgacatcatc atcaattgct gagggaatca
     2041 aaagattgct tgatgatgat catttaagag gaaaattaac aaacaatttg tcaaaagaaa
     2101 agctgggaac tgagtctgaa atagaaaagt tatataaatt gtttaatgca ggataggaaa
     2161 ggacatagaa atgattattc aaacaagtgt tttttattaa ttactctaat agtaatagtt
     2221 atactaatag atattattcc tatatttaaa gactggttgg ggcggattca catagggaga
     2281 tatgacaata aaaacatctg gaataaatta attactgaga aagcagttag ttggttaaat
     2341 gataccccaa agataaaggt aaccgataat acacgcttaa ttgtattaga tatgttaaaa
     2401 ggaaattata ctaaaagtgc tatacagcac tggcaagagg catcaatatt attaggttta
     2461 tcagaatatt taaaaagtat tgaagatcac aaagctgaaa atgagatagt aaagttttta
     2521 aacaaaaagt ttgatagtgc aggtcaatgg ataaagaagc ctgtacatgt agatggggca
     2581 atattggctt atgctgtcat gaaattggac tttatagaaa ctgatacgta cagaaaagca
     2641 ttggattata catgggaaat gataaaagag catataggcg aagaaggcac agttgaatat
     2701 aggaaatcta tgccgagcta cagatatgta gatacgatag gtttcatatg tcctttttta
     2761 attgcttatg gaacaaaata taataaaggt gaatgcatag aattagcagt aaaacaaatc
     2821 aaagagtacg aacgatatgg catgttagaa atatactcta tcccgtgtca cgcttataag
     2881 gtagataata aaataccctt aggattatac ggttggggaa gaggtcttgg ctggtatgct
     2941 atcggtctta tagattcatg ggccgaactg ccaaccgata gtagacataa atcgatacta
     3001 gaagaaagtg tgaaaaaatt cgctaaagct gctatgagct ttcagcagga taacggaagc
     3061 tggaactgga ctgtcactag aaatgaatct agagcggatt cttctaccac cgccacctta
     3121 ggctggtttt taattaatgc ttcaaaaatt gatgggattt ccaaagaatg tctagacagc
     3181 actaccaaag ccataaatta tctgatgagc gttacaagaa gaactggaga agttgatttt
     3241 tctcaaggag atacaaaaga tataggggtg tatgcaatgt tattcaatat actgccattc
     3301 acgcaaggat tttgcataag aatgataaat gctatatcta ttacaaagaa gtaaaatgcg
     3361 aatagaaaat cttattgaaa caggaatgaa acttggtgat aattggttaa ttaagacggg
     3421 aataatattt gtttactctc atatacaatg tccttccagc acctcaagtg tatttagcgg
     3481 tgctagagct ttaattatat cagaagtaac taataataaa aattcaatag ttattacatg
     3541 gaatttatag gatcatagta acgaattctt taacaggaaa cgtaacctag aaaaatccta
     3601 aaaatttaat gatactaaag ctaatatata agaaagtaat tatactataa aagaaaatgt
     3661 atcacgtatc agtcagaata aaatgcatga acagctaaaa agatgaaata gtttttatta
     3721 aataggttgt taaatataca ggagagtata aattttatat ttcttataca tgtgacctaa
     3781 aggagagcaa atgagaacta aacattcaat tattaacata gcaacgggtt tagggaatca
     3841 aattattatt acatcattaa gctttatttc aagaacggta tttataacat atttaggggt
     3901 cgaatactta ggaatcagtg ggctctttac tagtatatta gcaatgttag cattagctga
     3961 agcgggtgta ggctcaagta ttgtttatag cctttataaa ccagtagcag aaaatgatca
     4021 ggaaaaaata aatgttctaa tgaacttata taagaaagca tatatggtga ttgccttaat
     4081 tgtattactg gtgggcttat cgatcatgcc atttcttgaa ctttttacta aggatactag
     4141 tgtagaaaac attcatttaa tctatctaat ctttttaatt aatactgttt taccatactt
     4201 gtacttacac aaaaacaact ttttaagcgt atgtcaaaaa acatatatga taacaggaat
     4261 ctactctata tcttcgattg catctgtttg ccttaaaata ggtatattat attatactaa
     4321 aaactttatt ttatacttag ttgcagattg ttttataaca atagttacat ctgctgtttt
     4381 agccattatt gtaaataaaa tgtatccatt tttgaaaaat aaagtaacca gaaaattaga
     4441 cagcgaaact agaagcaaca ttattaaaaa tataaaggct attgtcttgc aaaatattgg
     4501 aacctatctt gtgtttggta ctgacaatat tattatatct acatttgtaa gtgtcacagc
     4561 agttgggttg tattctaatt ataatatgct aatagaaatt tgtaggactt ttgtaaatca
     4621 agtttttagt aatatttatc atagcgttgg aaatttaata gcacaagaaa ctaaagagaa
     4681 ggtttatagc atttataaag taactatgtt attgagcttc tggctatatt caactctggg
     4741 tattttgcta ctaatcatgt tagaaccgtt cattgctatt tggataggat ccaaattttt
     4801 aatgagtaaa ggtgtagtaa taatactaat tattattttt tatgaaagag gtatgagaaa
     4861 ttcaatttct acagtgaaaa caacttcagg aatttttcat gaagacaggt ttgccccttt
     4921 atttcaggca gctgttaatc tagttatttc aattattgtg gttcagcaca taggcattgc
     4981 aggtgttttt atcggaactc ttattagtgc cctagtcgtg cctttttggg taacacctta
     5041 tttagtctat aaaaaagtat ttaataaacc cataataatg tatttcgtaa gatattttta
     5101 ctttacaatt atagctacag gaacctattt tacgacaaat ttagtgtgga gtcttataga
     5161 acccgacact ttcgtaacat tagttttaaa gggaattatc tgtttaataa ctatcaatac
     5221 gatatatata ttagttttta caaaaccaag gagttcaaat atttatttaa tataattaaa
     5281 aacataatgg gaacgatatt agaacgattt aaaataaata aaaagttact tataaaagtt
     5341 aaatgttagt tttagcggta ataataattt catcattata gtatgtaata aataaaaaca
     5401 ataagataaa agagtactca ttattgcaaa tgaaaaatga ggaatatatt aaaatcaaga
     5461 aaagtaataa aacttcaaat ttagattgta tctaaataat ggtttgtaga gaaagtggca
     5521 aaattaaaag aaaagagtaa agagtaaaga aataattgca gaaatataga agactagaat
     5581 gatttaatta ttaaaggtat tctagtcttt ttgaattatt attttaggaa attaaaaatg
     5641 taatcatatt acttaagcgc attaaagtta agggctaatt cttcaggagt taatattcta
     5701 ttataatatg ctacattttt aaataaaact ggtactgctt tatcgcctat aatgagcggg
     5761 agggaggaag gtttagtata aacgttatta gctattcctg cttccgagac tttaacatta
     5821 ttgatataca ttaacaatgc tgtgccggtt cttattacat caatgtgata atacaaagat
     5881 tcatctgcat actgaggaga ggtaacggtt gtgttagcgg tatttcccca tatagaaaac
     5941 ccaaaagcat tatttccatt gcgttctaac aatattttat tttgagatcc acccaattca
     6001 tatctgctaa ttaagtcacc aaatttaggt acttttgcag taaaggaaac ggtaaatgta
     6061 tcattcacca taaaatagct gtccggattt tcaataatta aattgttgga aggattatct
     6121 ttatataaaa taaggccatt tgttacgtag ttagctgata cggctgaagt ctctactgta
     6181 acactaatac catcagaaac atttccgttt atatcttttg attttacagt taacttatat
     6241 tgtatatttg cttttaatcc agttatactg taattcgtag ttgtaacatt tccatttaat
     6301 aagacagagt cctggtatat ttcgtaactt gcaacatcag aggataagct tcgagtccaa
     6361 gttaatccaa tcgagtctga agtgatactg cttgctaata agtttgaaat ctcttctggc
     6421 ggtgtggtat cttgtgaagt agtaactatg atagaaatac cagatgattt atttcctgct
     6481 ttgtctaccg ttttaacagt aaatgaatat tgacttgcag ctagaagacc attaacagta
     6541 tacgtagtac tgttaatgga ccttataagt gtatccccgt tatatacttc ataatttaat
     6601 acatcgttag aagtactagc tgtccaggat aaatctactt tatcattaaa cggtgcagca
     6661 gttaaatttg taacattggt tggaggagtt ttatctatat taaactgaac tgatttaact
     6721 ttactttgat taccagcagc atctactgca ataactttta gggtagtggg atttgaaagt
     6781 ttaaggggcg tagaatattg aatactagat gtagttggaa gcgttccgtc taaggtatag
     6841 taaataattg catcttcatt tgctaataat gttaccgttt tatctgtatt atatattccc
     6901 ccattagggt taacagttac cctaggtgca ttaaggtcag ctgtggaatt agtgatatca
     6961 atgttttcta ataacaaggt tgttccagga tttttattta cagtcatagc tgtattaaga
     7021 agggtacatc ttgtaattaa atatcctttt tttagttcta gccagtaccc atcaactata
     7081 gaattttcta aatagcaatg gttcaaaatt gtttgggtgt tgttatcagt tttactgagc
     7141 tttacgttaa aaaatgaaca attatctaca gtagaattta ttaaagttgc atcgatattt
     7201 ctaaataggc tgtttttaat attaactttt gtttctggga aagctgaatc accaaatttt
     7261 aagtcaggac taattgactt gtttctattt ctgatgaatt gactatttaa tacattacaa
     7321 tctttaatat tcgcactacc gtctataata gttaaaccgt caactgttaa cggcctgcct
     7381 acataaatat tagatccgtt tccattaata cttacactat gatttttaac tgtgacattc
     7441 tgaataatgc tacctgctgc agttactgca aagccgggtg ttgttccacc actattccag
     7501 tctctttcaa taacaacatt attaatatag caccttccat aattaaatcg actaacatta
     7561 aatccccact cgccggaatc ataaaaggtg acaggagggc catcttcagt ttcagatact
     7621 tgaaaagaat tagtagtacg atttacaaca tagtatattt tgtcgacact aatactgcta
     7681 ggaacctttc ctacccattg ttcaaaacaa attttagccg tatttcccat accgtgatta
     7741 ttgataataa aagaactcgt tgaaacatca actgtaggcg taccaaaata accatagttg
     7801 gcatttccat aaaaaatgcc attatttaaa cgtatgtctt gaataacaga acctctaacg
     7861 tccattaatc taagattaga ctgttgacca actaagccat ttacataatt gtcaccagat
     7921 acaataaatt ctgtattaat aaaagtatta tttaaatact ttatatacat gtttgtagga
     7981 taagaactaa ctcctccaac attccaacct tcgaaaatat tattttctac ggttacatgg
     8041 ttaccatctg cgttcacaaa gtggcctctt tcattatcgt gaatatgatt gttaaaaatg
     8101 tataacctaa agttcagctc tagaccatct ttattatagt aaggctgttt atatggtatg
     8161 ttgctttcac ctaccataga ctcaatatct attgcaaaca taggaggaat tccattgcga
     8221 aagtctgatg taacattatc atctgcttta cctatatgat gaatcttatt atcataaatg
     8281 tatacatcgt ttgctgaagc aagagtaata ccttgtcttc tacagtggtg tatatcacag
     8341 ccatgaaccc aaatgtggtg tcccatttcg ttctgagtat agtcagatga attagttcgg
     8401 tattgtaacc aacctgttga aacagcatct ccagtacaat cacatatctc tgtgttttta
     8461 atttctacat gatttgaacc tgctatttcg attccttgtc cccattcatg agtatatctt
     8521 tcattatcaa ctttcgcagt aatagccgca atttgattat ccgttaatgg ggtattagta
     8581 atatcaatag tgaaaaccat tttgttgttt gctccaatat taggctgtaa gagccatcct
     8641 ctgcctgttg gtgatgatgg agcaaattgt ccattatttc taaaaccaac taatgtgtta
     8701 ttagaaactg tgtctttgta ctgataaaag ctatagcctt tagatacatc tatcccttcg
     8761 attttccata gcctaaaatt gtttaaaagt cccggatcct taaatctatc aattatttca
     8821 cttcttatga aattaagatt atcgtttaat gatccatcag ggttgattcc accccgaaca
     8881 aatttcacac caatttcgta ataatgtgtt tttcggtctc cgattatctt accgccagaa
     8941 atctttacgt ttttgacatc tttaacttga aaaatactgt agcctggaga actgttacct
     9001 tctaattcta gtatgcaacc atctgccatt tcaaagtgaa ggtcatttac catattaata
     9061 catgtgaagg ataatgaagc agcatcttgc catacccatg gttttagctt tagtttataa
     9121 aaaccactag gtaatattac atggttaacg ccttgagact tagcccaaat aattgcatca
     9181 ttgataccct gggtagtagt tttcgaagaa gttccatcgt ttttaatatt ccacctattg
     9241 agttcaatta cataaggaga tgaggatatt gtacctgata aatttgttgc tggtttccaa
     9301 atgccggctt catatttgag aacatcccca tttttaggtg ataatgtaga tatatctacg
     9361 tcactcaact catctaaact agtggctccg tgattgtcac taatttttct ttctatttca
     9421 ttaattgcat taactaaatt ttctttatcg atagttgtta aatttgataa aagcccaatt
     9481 tcacttttat ttgctatttg gatattacca gaatcagaac cgataaataa ttcggctgta
     9541 tctgtgcata atccaaattc cccaatacct aaaattggta aatccttctt taaactactg
     9601 ctgatttgta tcttatatgc caatgcaaat ccccccgtta attgttatag ctttaataaa
     9661 aagagatgct catctttatt ttttccttat aatattaaat tcgaaatttc ctcttattgc
     9721 ttggactaaa cggtaattat tgtgaaatta tggtgaattc atgtgttaat taaaatatca
     9781 tttgaaattt tcttctgagt cattgacatc tgatgtaaat aagatataat ttacctatat
     9841 tgttctttat aaagaacaat ataggttgga tagagaaatt gatttggaat ttataaagtt
     9901 aaattaaaac gatttaagat ataaaagtaa attattttaa atcttagttc tttaaaaaga
     9961 ataagttcta tcatgataca tatttgaaag ggagaaaatc atgagtgcaa ttgctggtat
    10021 ttatcattct aataatgaac ctgtacctgt cgagcatgtg aatagagtta tgcaatcttt
    10081 acaaaaatat cctgctaatg atattcaaat atcgcataag aaaaatatct ttcttggttg
    10141 ccatgcacaa tggattacac cggaatcagt gggagagcag cagccttatt gtgattatga
    10201 aaggcagcta gcaattacag ctgatgtcat tatcgacaat cgagatgaat tattcgagaa
    10261 actgcaggtt aaatacattg aaagaaaagc gatgacggat agtcaattaa tcttacttgc
    10321 ctattacaag tggggagagg agtccccaaa gtatttagta ggtgattttg cttttgtaat
    10381 ttgggatgag agaaagcaaa tactctttgg tgcgagagat ttttcaggaa gtagaaccct
    10441 ttacttttat catgatacaa atagatttgc tttctgcact actatagaac cattgttatc
    10501 ccttccctat gtagaaaaga aattaaatga acagtggctt gcggaatttt tggcaatttc
    10561 tggaatggtt gatgtagtag acgcatcact aaccccttat aaaaatatag cacagatgcc
    10621 ccctgcacac agtattaccg taatggaaga aacagtaaag gttaagcaat attgtacatt
    10681 aacttccgtc caaacattaa aattgaaatc taatgaagag tatatagaag cgtttcaaga
    10741 agtgtttcaa gaggcagtta cttcacgttt acgcacttat cgtcaagttg gagctcagtt
    10801 gagtggaggt ttagactcag gttcaattgt tagttttgct gcaaaaagtc ttagtgggac
    10861 acgtaagcaa ctatatacat ttagctatgt tcctcctaat gattttaaag attttacacc
    10921 taaacactta atgccaaacg aaacgccctt cattcaatca actgtacagc atataggtgg
    10981 tatcaaagat cattatttag attttgaagg aagagattcc tattcggaaa ttgatgattt
    11041 tctcaacact atggaaatgc cttataaatt ttttgaaaat tctttctggt taaaagggat
    11101 gtttgagaag gctagtgagg aaaatgtagg agtgctgcta aatggcggta gggggaattt
    11161 aagcatatcc tggggttcag ctattgatta ctatgctctt cttttaaaaa aattaaaatg
    11221 gattcgatta gctagcgaac ttcaccacta tagccacaaa gctggaggaa gtagatttcg
    11281 aagaatccca gttattgcaa gagtagcgtt tccttttatt gagcgacttt ttccactagg
    11341 agattcatat gagtttccga tgctgattaa cccagaattt gcaaaaagaa caggtgtttt
    11401 tcataagctg caaaactacg gtatagatga aacaggatgg ttttcagcaa caaatatata
    11461 tgaacagaga aaaaggcatt ttgaagatat ttttcattgg aatgcaagta atacacttgc
    11521 aaccaaatta tctttgcgtt acccattatg gaagcgggat cccaccaatg atcttagagt
    11581 gattcgtttt tgcttgtctt tgcctgagga acagtatgtt caaaaaggaa tggatcgagc
    11641 tctgattcga agagcaacag aaaagctttt acctgataca gtgaggttaa atcaacgaat
    11701 tcgtggtgtt caaggtgcgg attgggtcca tcgtatgatg ccgcactgga gtgaatttac
    11761 ggatgagctg caccaactta gtaaagatca aacaattctt gaatttttag atgggaaagg
    11821 aataaaagaa gcgatgtcta atattcatga aggcgtgcat gcaaagcatg caattaatcc
    11881 ttattataag ataatgatgc gtagtttaat cgtacatcgc tttataaaaa aatttaattg
    11941 agaggaggtg agatgatgaa gaaagagtgg caaatacctg tattggaaac gttagatatt
    12001 acaatgacaa tggcttctac agtctatggt ccttatacgg atgaagcata tgtaccaggt
    12061 aaagatgttg ataaagataa ccctggtaca tggaaacgtt ttacgagttg agacaaataa
    12121 aatataaaat ttgatcttat attaacctct cttgtttcat ttttctcgaa tttttgaaag
    12181 ccccttatac ataatagtgt atttggggtt tttattttaa aagttagagt gaggtgctaa
    12241 attctgattt tcatatccta atgaaataaa taaaataggc agttaagtaa gttattgttt
    12301 agaatattta ttactcaaga tataggtagt aattttaagt gattgacacc atcaagaaaa
    12361 ggtgataact ttattttaga tattgttctt tataaagaat aactttgttt taactaagcg
    12421 attttttcta acatattcac gtaaaagtat ttttttatct ttttgttctt tataatgaac
    12481 ttaagtgtgt gattttatta ggtaatgact atgttgttga aaaagtacag gagggtatct
    12541 attatgaaaa aattatttgc aaacaaaggt gagtctcaat gatagatact accaaaaagg
    12601 ttgtgtatga ggcatttgga ttaaaattac tcagtgaaat tcctttacct gaactacctg
    12661 aaataagtat ctcgggagga tcagtagaaa tcatagttaa gaagggggat ttaaccacac
    12721 tatggtcgga aaaaaataaa tcaggcgcgt attttatcgt gaaagaaaac ctgtgtatgt
    12781 ttaaagttcc tggtttagct atttatctaa ttcaagatgg taaagagatt acggtttctc
    12841 cgacagaggg ctttaatgaa gacttgagtc ggctatatat tctaggaaca tgcatgggag
    12901 cattattaat acaaagaaaa atcttcccgt tacatggaag tgctatagct attgatggaa
    12961 aggcctatgc agtagtaggt gaatcgggtg caggaaaatc aacgcttgcc tctgcttttc
    13021 taaagagagg atatcggctt ctaagtgatg atgtcatacc cgtaacgttt actgaagaga
    13081 atattcctat tgtaacgcca gcgtatccac aacaaaaatt atggcaggaa agcttgactc
    13141 aacttggaat ggaatcaact tgttaccgtc caatctttga ccgggaaacc aagtttacta
    13201 ttccagtaga atctcagttt gccaccgaag cttatcctct tgctggtata tttgaattaa
    13261 ttaaaaacga cagtaatgaa attaaaattc agtctattga aaaattagaa aggcttcata
    13321 tgcttttcaa ccatacatac cgtaattttt tcattgaact ctctggttta atgaaatggc
    13381 actttgatgc atcagcaaaa attgttaata aagttgacct ttaccgattg catcgaccaa
    13441 tctctggttt tacggcttat gatttaacag acttaatttt aactacaata aaaaggagag
    13501 aaaacaaatg attaaaaatc aagacatttc gatgaatcaa ttagttagtc aagaaacagg
    13561 ccatatcgtc agtgacatgg atggagaaaa agtgatgtta agtatcagca atggtaagta
    13621 ctataattta ggtgaaatag gtggagaaat atgggagaaa attaaagaac ccatttcagt
    13681 aagcgaatta atttctacat tagtaactca atataatgtc gaacaatccg attgcgaaga
    13741 acaagtcatt gcttttttaa gtgggctgat aaaagaaggt ttaattcaag tagcagagaa
    13801 taatcgctct tagtttcaat gtttttttat ccgtgggagg gaaaaatgaa aattttgcga
    13861 aaaataacgg tttttttaaa attgaacatg agagcaaaac tgttgttgat agaggcattt
    13921 atttatttag gatgggcccg ttttcttaaa acgttacctt tttctaaaat atctccttcc
    13981 ttaggacagc aaatgaagga aacagcgttc actactagtt catccgatga taaagttcta
    14041 aaggatatag cccaatctct tcatattatg agccgttaca ctttttggga aagtcaatgt
    14101 ctcgttaaag caattgcagg aatgaagatg ttagaaaaac ggaagattga aagtacactt
    14161 tatttaggta ctgctaaaga tgaaaacgga aaattaattg cacatgcatg gttgcgcagc
    14221 ggtccatttt ttatttccgg tgcagaaggt aaagcgaaat ttaccgttat agctaagttt
    14281 gcaaaggaaa tcagtgtgaa aaagcgtgaa ggagaaaatt atggataaca tactcaactt
    14341 agaccgcatg cctaaggaat taaaactaat tcttgaatta ttgaaggaag ggaatgaaca
    14401 ataccttgaa aaatatagcg aaggattatg tcgagatatt gactggaacc tttttatcaa
    14461 actgacgctg catcatcgtg tttatccagc gataaatttc aaaataaaaa tgttggatgc
    14521 ggatgtagtc ccaacctatg tagttcaatt tttaaataga caatataaaa gaaatacatt
    14581 tcaaatgctt cgtttaagtg cagaaatgga aaaagttagc ctgctctttt ctaaacatca
    14641 aatacagact ctatttttaa aaggtcccat tttggctaaa tgtttgtatg gagatatctc
    14701 tcttcgtact tccagtgatt tagatttcct tattccaatt caagatcttg cgaaagcaga
    14761 agacctttta gtaagtcttg gatatgaaaa ggatgattat attcacactg tattaaatga
    14821 ctggaagtgg aggcatcatc atgtcacata tattcatcct catacagaaa taaaactaga
    14881 aattcattgg agattacacc caggtcctgg aaaggaacca tgttttgatg aactatggaa
    14941 gcgaaaagag aaaagttcaa ttaccagtta tcctgtgtac ttattaggca cagaagacct
    15001 atttcaattt cttgtggctc acggagcgcg tcatggctgg tctcgccttc gctggctgat
    15061 tgatattcag caaatagtga aaagaaaaat tgattgggta aaactttata ctttatttac
    15121 gacttatcat aatccttatg tagggggaca ggcaattatt ttggcctcac agttgttaaa
    15181 cgtgagggta gaaaaaaaaa tgaaaccttt attaataagc aaacgatccc aaaaactagc
    15241 tcaagaggca gtcttttatc ttgaaactat gataaattta catactgatt ccgtgcctga
    15301 atatgtatcc aaatatcata gtcgccattt attctcatta atgtctgctc aacaaaaact
    15361 tttctttacc gcaagtttat tgcatcctta ccctgaagat gcagagatgt tacctttgcc
    15421 taaaaaactt catttcttat attttccttt gcgtcctgtt ttatgggctt ggaggaagac
    15481 taaaaaagat gagttacgta aggggagaac ataatgaaac agcttgtata cttcttgaag
    15541 caaatacatt cgtttgctgg caaaaggctc tatattaacc tcttagcaac ggtgtttatg
    15601 agtttacttg aaggtatcgg gattttatta ttaattccga tgattagctt aagtggtatc
    15661 gtggattttg atacaggagg accatcaatt ttaagggtat tttcattttt acaaaatacc
    15721 cctgtatcta tgaccttgct tctaattttg agtatttatg tgttaatagt tactggtcaa
    15781 actatattac aacgacagat aacgattcaa aatagagtca ttcagcttgg ttttcttaga
    15841 catatgcaaa tggagactta tagcacctta cttcatgcga actgggagtt ctttataaaa
    15901 aaaagaaagt cagatcttat taatatagta cttacagaaa ttgctcgtac tagtggagga
    15961 acaacctcgt ttttgcagtt tatagcttct cttatattta cgtgcataca aattgggctg
    16021 gcattttggc tttcacctaa aataactctt tttgtattgc tttgcggtgt agtgattatc
    16081 tttttaaacc gtaaatttct caaacgatcg ctgacattgg gcaatagaaa ctttgagctg
    16141 ggaaaaagct atcttgcagg gataaccgat cagattaacg gaattaaaga tattaaaagt
    16201 aatactttag aagaatcgag aatgaactgg taccgatcaa ttacagaaga aatgaaaaat
    16261 gagcaggttg aatatactaa gctaaataca acgtcacaat cttattataa aatctcatct
    16321 gctgtattag ttgcagcctt tatatatgct gcagtgaagt tgtttcatgc tgaggcaagt
    16381 gagttaatgc ttattattct aattttttca aggttatggc caaaggttgc cggtattcaa
    16441 gcctctatgg aacagatttc gacaactcta ccagcattta aagctgtgaa agccttgcaa
    16501 gatgaatgca aaaatgcaag agaatttgaa aaagaagcta atcaaagtgt cgaaccagtt
    16561 caatttaaaa aagacattca gtgcagaaac gtttattttc gctataacca aggtgcatct
    16621 tacgctttgc agcatattaa tttaatcatt ccatctaatc aaatgacagc ttttgtaggt
    16681 cggtccggtg ctgggaaaag cacgctgata gatttgttga tggggcttaa tcagcctgaa
    16741 aaaggtcagg ttttaattga tgggattccg ttatcaaaag ataatttatt atcgttaaga
    16801 cgagctatta gctatgttcc acaagatcct tttttgttta atgcgagtat aagagaaaat
    16861 ttactgttag tagaacctaa cgcaagtgaa cagcgaatgt gggaagcgct tgagttttca
    16921 tcggctgcag aatttgtaag gaggcttcca caaggccttg attcattaat cggagaccga
    16981 ggaattaagc tttcaggagg agaaaggcag cggcttgtgc tagcccgcgc tattttacgc
    17041 aatccatcta ttttagtctt agacgaagca acgagcgcgc ttgacacgga aaatgaagca
    17101 aaaattcaag aagcattgga acgattaaaa ggaaaaatga cgattattgt tattgctcat
    17161 cgtctatcta caattcgcaa tgctgatcag gttatcgtgc tggagcaagg taaaattatt
    17221 caacaaggtg gttttactca acttgccgaa gaaaagagta atacatttag tcaattacta
    17281 ggaaaacaaa tggaagctgc tcattagaac tttactaaac agcaatgggt taaatttttt
    17341 gaatcaatcg ttcttaataa agaattaatt gaaaggaggt gatcatatgc aagagaagac
    17401 ttatagcgct ccagaagtgt taaatcatca gctaatccgt tttgaaaccg ctcaaagctg
    17461 gaatcctgga aaaggaaact tagatcatcc tggaaaagga aacaatggtt ctaactggcc
    17521 gtctacaaat cccaatccta ctccaggtgg tgcaggtgcc ccaggaaaag gaaaagggaa
    17581 aaataaataa aatatttttt cagctatttt atttacttaa tacagcaata tataaagcag
    17641 aaagtcaatg gtgttccatt gactgctgtt ttaagagaaa ttgattgagg agacgaataa
    17701 tgaagacgct gcatacaaaa ataggggatc actatattga tattaaaaca gaatttgatg
    17761 acgtatggaa aataatcaaa caatattttt tatgtgttag tatagcgaaa aatcagccgg
    17821 acatgacggt cacaatcaca aatggctatg gtgtttcgtt tcaagattat gaagtcgcta
    17881 tagaagaaaa actcaataca attactttta aaagagcaga ctatttaatt gaagtagatt
    17941 cagagtataa actggctaac gtagctatcc acgatgaatt ggctttaaaa catgctctta
    18001 tgaacttata cagttcgttc attgttcatc ataactgggg gattcttctc cattcatcgt
    18061 gcgttattaa cagcggcaag gcgcatattt ttacagggca ctcaggagcc ggaaagtcga
    18121 ctgcagcaaa gttatcaatg ccaagggaac ttctttctga tgaagctaca attttaagaa
    18181 ttacagacga gagtgtaaca gtttttaact caccgtttag aagtgagtta aaagccaagg
    18241 gaagtgaaaa aaacacacct ttagaaagta ttcagctact gcatcagtca atatctaatc
    18301 aacggataga ggtaaggaga atagaagcgt tgatgaagct gatagataaa gtgttttact
    18361 ggtcttataa acctgaagaa acgaaaacaa tcatgggcct gttacaattg ttagttaaac
    18421 aagtgcctgt gtatgagctt cattttcaaa aaaacaatac attttgggag ttgatgtcat
    18481 cgtgacaagt tatattcaaa agggcggtta tgaagtaatg gaagtagact cagagtgggt
    18541 gattttaaat tcgcatgcct atacagtgac tactttaaac gaagtaggag gctactgttg
    18601 gaaggtaata gaaaacaaaa aaacgcttgc ggaaattatt aaagctgttg cagcttatta
    18661 caatacagaa gctgaaatgg ttgaaggaga tatacagcat tttttaaata agttaattga
    18721 ttgcggcctt gttgaaaagt atgcaagcta ataataagca ggggcatcta ttaaaagctt
    18781 tgattgatcg gaatggattg cttgaacttc ctgctgaagg aacaagtatg tatcctctta
    18841 ttaaacaagg agatatttgc cagtttactt caataaagca ttctccgcta aaaagaggag
    18901 acattgtttt atttgaaagt ccagctaata aactcattgc tcatcgcctt ttaaactgtt
    18961 cctacgttca agaagaagaa tattatattt ttaaaggtga tacgaactac ggggtggatg
    19021 atctggttac ccgagaaaag attatcggaa aacttgttgt tattcaaaaa aaatcagtgg
    19081 cgattcaaga aaaaaatgta attgctgttt tatggggaaa gctcattctt gcttttcctc
    19141 tcctgtcagt gctgttacga aagtatataa actatcaaga atggaaaaaa gcagattgaa
    19201 aggaggatgt cgttatgatt agtttaaaaa agataaagca attcgacatc cgcgaatata
    19261 tttctatcaa agagacaaaa agtatggttc agtggctctg gccatacgta atgaagcata
    19321 aaggtgctta tattggactg tttattttgt tatttgttga aattaccctt actttagtat
    19381 ctgcatggtt tatcggaaac attaccgatg ctgctgttca tggtaatttt gaaaggctta
    19441 agtggctagt agctatcggt gtagggttgt cagtaacaag tattacagct aattttattg
    19501 atacatatct tgaaacaatt gcggtcaatg ccgtcaagcg agacttgaat ttggatttat
    19561 ataaacatat tttactttta cgagaacgag acgtttcaaa tttacattct ggtgaattgt
    19621 tgtctcattt cactaacgat atacataatg ttgaaggtgt aattgggcga ggtttaatta
    19681 atttagttag atttccgatt atttctatcg ctgcctttat ttatttagtt caaattaatt
    19741 ggaaattatt gttatcaatt gtgctcgtat ctcctattgc gattgctggc ggagctatat
    19801 ttgggatttt acttcgaaat aatagccgta tcatgaatga attaattagc aatattatta
    19861 aaaaactgaa tgatacgttt cacggattta ctgtaattcg atcttttaca ttggaaaaac
    19921 tgttttatag agattataga caaaagaacg agcagcttta ttcattggaa atgagagagg
    19981 caaagttaag aggctggttt tacgctggaa gcgaagcaat tggagccatt gcgtttctta
    20041 ttagcttaat tataggtgct ttttttgtaa cagacaagat tattacgatt ggtgcattat
    20101 taacgtttat taaccttgtg aaccagttga tttctccctt gacaggacta gctggacagt
    20161 gggctggcta tcagcggtct gtgtcagcgt tagaaagaat tactaaacta cttaattatc
    20221 caactcaagc gaaggcgctg cctcactata cttccgcaac gcttgttcaa aaggatatac
    20281 aggttagaaa tcttacattt agctatgatg agagcgcgat tgtattcaag gatttcaact
    20341 tatacattcc tgctggtaaa gcagtagcgc tagttggtcc aagtggtgcc ggaaaatcaa
    20401 cgctttttaa tttgattcaa catttttatg aacctcattt tggcactatt tcaatcgatg
    20461 gacgttcaat tgatgagtta accatttcag aactaagaag ttcaattgca tacgttcctc
    20521 aagagacatt tctttttagc ggtacgataa aagataatct aatgatagcg agacctttgg
    20581 ctacggaaga agaaatgatt aatgccgctg ttcatgcaaa tattcatgat catattatga
    20641 cgctgcgtaa cggctatgaa acagaagtgg gggaacgagg agctaagctg tcaggaggtc
    20701 aaagacagcg attagccatc gcaagagcca ttttaaaaga tgcgcctatt cttctattag
    20761 acgaagcaac ttcagcacta gacagtgaaa cagaacattt ggtaaaagaa gcattggccc
    20821 gtttaatgat aaatcgtaca actatcatga ttgcccatcg attgtctact attcaacatg
    20881 cagacctcat tgtggtaatg gatcaaggag aagttgttca aaagggcacc cacgaagagt
    20941 taatcagaca aagtggaatg taccgcagat taaataacgc acaagaaatc agtcaacaga
    21001 atccaatcat gctgaaagct aaataatagg aaggaagaag tgctttgctt gataaactta
    21061 taacacacct ttacgattcg aacgctaatc taccaagtga tgatgaattt tattatgaag
    21121 taataaaaga gattgaattg tatttcattt ccccacaagt ttactcctta ttgaaagaaa
    21181 aaggagagct aggaaatact cctttatttt ttcaaacaag actcaagcaa aaatatcaag
    21241 aaacactata tcaaaattta tttattaaaa atcaaacgga tcatattatt cgaaaatttg
    21301 aagaagaaca gatagaagtg atacctctta aaggagtcta ctttgctgaa aaatattttg
    21361 gtcacatagg tgctagaggg acttcagata tcgatatttt agttaaaaga aaagacatca
    21421 ataaagcaat agaagtagta aaatctatgg attttaaaat tgaagaagaa tttattcctt
    21481 ctcactttca cgttagtttt agtagagaaa ttcctggatc ggctataccg ctaacagttg
    21541 aaattcattg ggatatatta aaagaaaaaa cggctaattt taacatccaa gagttttggg
    21601 gtgaagcagt aaggttagaa ggttttcagt ttgtaaatgc tttatcccac tatcatacgt
    21661 tttatatgat atgtcttcac ggttggaggc acaacttaga ttcgcctaag cattttttag
    21721 atattattca aatgattgat gttgtaaagg ataagataag ctatgaaact ctctttaagg
    21781 atgcaaagcg tcatcaaaca tataagagga tgattcgaac attgtccatc gtgtatagcc
    21841 agtacagcat attggaacaa ataaaagaat taagttctat tgaaaatcaa tttaagggtt
    21901 ttgcgtcttc aacattggaa caagaaaaga aaggaataaa aaaatatttt acctttatgg
    21961 actatcaatt ttttagctat gattctgttt ggcatagttt aagtgaaatg cgcagctggt
    22021 tattaccgaa taggtttgaa gtaacttctc agttaaaaga gacgtcaaaa caacattcgt
    22081 atttttatag tttacttatg ctatataaac agagatgtaa tagtgtggta aaagttctat
    22141 ggtcttaaga ttcgcattta tggagatttt gactcacgag gaagcctgtg ctagttttta
    22201 atcttaatgc cttggagggg atgctagtga gaaagatgaa aggaaaagtg atagcagcct
    22261 ctttcttttt aattgttgta ttaattggat atacagtttg ggataatcaa cgaattactg
    22321 tagctaagca agacatcata attaagcact tacctaaaga gcttgaaggc ttttctattg
    22381 ttcaaattac agatttgcat gaaaaagaat ttggagaaaa tcaagaaaga ctcataaata
    22441 ctattaattc gattcattat gatgctattg tttttacagg tgacatgatg aaaaataata
    22501 gtgaaaatgc ggtgccattt tatacgttaa tagaaggtat tgaaaataaa caacatgctt
    22561 tatttgtaca tggaaataca gaccccaaaa gctaccgtac atcttcaagt aatcttgaaa
    22621 aaggtaaatt tgttaaagat atggaaagta aaggtgtgag gctgcttgat tccacgtata
    22681 cagtaaagag aggaaacgcc catattcagt ttgttaactt tgattggtcg ttaaaaacag
    22741 agaataagtc aaaaaccagc ggaagtcact tatcaaacaa taagggctat ccagaaaaag
    22801 aaacctcaga ccttgttatt gggttgaatc actttccagt agttgattct cacatcgatg
    22861 ctcttcttag agatcccagc gttacattta ggtattatga tttaattatg gccggccatt
    22921 atcatggagg gcagtttcgt attccttttt tcggtgcact ttttgttccg gaagcttggt
    22981 atgaaaagag tggtctgttt cctcctcaag accgagttaa aggcctgtgg gaatacaagc
    23041 acattaagca atacgttagt gcaggtttag gaagcaatgg ccccatccca tttttgaatt
    23101 ttcggttctt tgactcgcct gaaatcaacg tactaacatt taaaagtagg aaaactgaat
    23161 gagaaaaata aaagtgttgg gctgaatttg aacagtctgc ttataaatta tgaggtttat
    23221 taacaataaa gctacgctcg acaaggaggt agcttcttgt tagtttattg aaaaagagga
    23281 agcctttctt cttgaatctt taagatagct gaaaaatttt ctataaaaga agcttcggtc
    23341 atcgcttgtt gatcctgcaa atcttctact atttttcttc gtgcattttc aaattttttt
    23401 aattgtccag cttctaagga accgtgagaa agtaatttag gtaaaatctg agaaagaaca
    23461 tcagcatcca ttatagcggc attcatacca tatgcgcccg ttggagtcat ggtatgggcg
    23521 gcgtcgccaa tgatagctaa tccgtctttt acccaagtat cacagtgaga gctataaaca
    23581 gacaataaaa tgaaattttt ccatgaatga aggttctgct caatactttc ttttaattga
    23641 ggaaatgcca ggattagctg atcaagaaaa ggctgtacag actgcttctt tagggaagta
    23701 taggaccctt ttttaatatt ccagcctatt tgaataaagc cgcccgtctg cgtaaacagt
    23761 gccatttgct gattgttcac taccgtaaag cgaatagtag gtttccattt ctcaggtgca
    23821 ggaattttcg cccaaagcaa atcgtatcca ggattatgaa tattgttttt tatgtgcgca
    23881 agtttgcgaa cagttgaata tcgaccatct gctccaataa tgattttact ttcaactatt
    23941 atttcacccc ggcctttttg ctcagctttt attccagtat actgtccttt ttcattctgc
    24001 atgagttccg ttacttttgt gcccagcata agtttgtagg agggatagag gcttgcttgc
    24061 ctatttatag actgaagaag atgctgctgt ggaacatgaa ttcctgcatg tccaagttca
    24121 gattctatcg ttttgaatac atgtccatcc ttccagtatt cgaccctaga cattcgaagg
    24181 aggcctaagc tttcaatttc ttcaaatata tgatgtttct taagaatcat ttctccatct
    24241 tcatttaaat gctcgcctcg aaattctttt ccaagtttat cagatctctc tatcagtata
    24301 gttgatatat tttgtttagc cagtagatag gccagtagag caccgcctgg tccggctcct
    24361 attatacaca catctgtttg atagttcatt ttcttcacct tatttgttat atgtaattaa
    24421 gttactttta gtaagtatat taacttttat aaatgttaat gtcaactttt ttatagcttt
    24481 tcttctatgg aattcgctct ttggtcaaaa cacaaataag cttttacata atcaaagtca
    24541 aagattaagt tttatggtaa actaaaatac acaaattatt ttgaataatt tgtataaaaa
    24601 ggtgaaattt caaaattgtt gagaaaaagc acggttggaa agctgcagga tttttagcaa
    24661 gaagagtaga gaacgtctct gaaaattcta gcaaactcca atttcgactg gaggatgatt
    24721 atgacaaaag tagaacatta tattcaaaca ttgggtaaca gtgcagattt gctaacgaaa
    24781 aagcagacaa gcatttactt cgaaaaactt tcaaacacct ttcctttttt aacaatcatg
    24841 caaattaatt ggagaaaagt attaattaaa aagtcggccc ggcatattga agaaatcaaa
    24901 aaatggcttc aagagatgaa tataaaagaa catcaggttg ttttattttg gaaacgagca
    24961 actaaagccg taagcgtcga tttagctcag gctttaacgt tttttcaaca aacagctgat
    25021 cttacagaag aagcgtttat ttactgtccg tctgttgact atgtcattga atattttaaa
    25081 gacggtaaaa tgatgattgg tttagcagca cgctaattaa aagggaaaaa agctgaggga
    25141 agaggcgaat gggaacttct tcactcggct ttttatgcat ataaactcta aaaaatacga
    25201 aacataacgt tatttacaac ataaattacc ttctctttat aattagtggg aaaagtattg
    25261 acattttcca taaaacaaat ataattaaca ttaatcatat tgttaattgt aaaaagagtg
    25321 atcaggagaa atgctgaatg tcgaacaaaa aagcagtgac gaatcaaacg aaaaaagaat
    25381 accctaaacc ctatggttat acgtcagata ttgctgtatt tacgattgtg acggaagaaa
    25441 aagaaccata caaaccacct aaaatgaatt tgaaattaat gcttatccag cgagcaatgc
    25501 taaacgcaga gggagaaaag aacagcgaag caggaaagtg ggcgcttccc ggcggatttg
    25561 tccatcctga tgaatcagct tttgaagcgg ctaaaagaga gctcgaagaa gaaacgggcg
    25621 tgaacgatat tcatttaaaa cactatggtg tctatgatga gcccgacaga gatgataggg
    25681 gatgggtcat cacaaacgca cattatgcaa ttgttccaga agatagtcta ataaaacgaa
    25741 aagcgaatga tgatgctgct cgagttgaat tatttaaaat agatgaaatc ttttcattgc
    25801 ctttagcttt tgatcatgaa atcatcattc gagatgcaat caaggaaatt aagaaagatt
    25861 tattgcaaac gaccattgct cagaagtttc tgccttctca gtttacatac tctgaactgc
    25921 aggctgttct gcttaccgta acggatgatg cggcaattaa aagtgatcag gcgtttgcac
    25981 gaaaaataag aatgctgcca tttattgaag aagtagacgg aaaaacaacg acgagaacgt
    26041 ctaaaaagcc aacaaagctc taccggtttg tcgagatgaa tgtgatgaag ccaatctata
    26101 cagcacgcta ttaaaagaaa aaactttcaa ttcaaataat taacaatatg ttaactgtta
    26161 atttttactt atgaggggga aacataatga aaaaagcact gattaacatc gattatacaa
    26221 atgattttgt agctgaaaac ggagctttga catgcggaga accagggcga cacattgaaa
    26281 catccataaa cgatattacg cgtcaattta ttgagcaaga cgagtatgtc gtgtttgcca
    26341 ttgattttca taaagaaaat gattccttgc atccagaatc agcgctgttt ccgcctcata
    26401 atattgaagg ttctgcagga agaaagctat acggaaagtt aaatgattta tataacgtgt
    26461 atcaatcaaa ggcaaatgta gaatggatga ataaaacgag atacagcgca tttgctggaa
    26521 cggagctgga gattaaactg cgagaaagac aaattaccga tgtctactta gcaggtgtat
    26581 gtacggatat ctgtgtactt catacagcag tagatgccta taataaagga tttgctattt
    26641 atatttatga aaaagctgta gcgagcttta atcaaaaagg ccatgagtgg gcgcttgaac
    26701 actttaaatc ttgcctcgga gcaaaaatta tttaaggaca taaaagtgga ggaaatcaat
    26761 gaatatgttt acagatgaca gcgtaatgct acacaccgat ttatatcaaa ttaatatggg
    26821 agaaacgtat tgggaagata acattcatca aaaaaaggct gtttttgaag ttttcttccg
    26881 caaacttcct tttgggaatg ggtatgctgt ttttgctgga ttagaaagaa ttattgaata
    26941 tattaaagca tttaaattta gcgaatcaga tttaacctac ttgcacgaag aattaggttt
    27001 taaagaggat tatttagctt acttaaaaga ccttacattt acgggaacaa ttcgatgtat
    27061 gcgtgaaggt gagcttgtgt ttcataatga accaatttta cgtgtggagg cacccttaat
    27121 tgaagctcag ctcgtagaaa cagcgatttt aaatattgtg aattaccaaa cgctgattgc
    27181 gacgaaagca gctcgaatca aacatgtagt aggagatcag gtagcaatgg agtttggaac
    27241 acgacgcgct caagaaatgg atgctgctat ttggggaaca agagcagcct atattggcgg
    27301 atttggtgct acttctaacg tgcgagcagg aaagaaattt ggaattccag ttgcaggaac
    27361 gcatgcccat gccatggtgc aagcttataa ggacgaatat attgcatttc ataaatacgc
    27421 tcgtcgtcac aaaaactgcg tgtttttagt tgatacgtac gatacgttaa aatcaggagt
    27481 tcccaatgcg attaaagtag caaaagaatt aggtgataaa attaatttta cggcgattcg
    27541 cttagacagc ggcgatttaa cttatttgtc taaaaaagct cgtcaaatgt tggatgaagc
    27601 agggtttaca gaaacaaaaa ttgttgcttc caacgattta gatgaagata cgattatgca
    27661 tttaaagtcg caaggtgcac agatagacat gtggggaatt ggtacgaagc tcattactgc
    27721 atatgatcag ccggcgcttg gagcagtgta taaactcgtt tctattgaag gagaaaatgg
    27781 cgagatgatg gatacgatta aaattagcgc gaatcctgaa aaagtgagta caccagggtt
    27841 aaagcgcgta tatcgtatta tcaataattt gaatggaaaa tctgaagggg attatatcac
    27901 gatggaagaa gaagatcctc aaagcgaaga acgtttaaaa atgttccatc ctgttcatac
    27961 gtttatcagt aaatttgtca caaattttga ggcaaaagaa ctccatcatg atatctttgt
    28021 aaacggaaag cttgtgtatg aaacagaaga acttcaggaa attcagcagt acgtaaaaga
    28081 taatttagag ctgctttggg atgaatataa gcgcacgcgc aatccagaag aatatccagt
    28141 agatttaagc gaaaagtgtt gggaaaataa aacgcgtcaa attgcgtacg taaaacaaaa
    28201 agtagcggaa gccctaaata aaaaatagta gggagctgga gacatgtcag atacgcttgc
    28261 ttattttcaa cagctaaacc ctgataagaa aatagataag cttttagaaa aaggagcgaa
    28321 aattgggatt tatggttctt cttttgatcc ggttaccaac gttcatcttt ggacggcatc
    28381 aacggttgcg catcgaaaga agttagatgc tattatcttc ttgccttcat cgcacaaacg
    28441 gacagataaa aagctccaaa cgtccgatga gcaccgtgta aatatggtta gcttagcgat
    28501 taaagacaac ccgaaatttt tgctcgatac gtatgagcta gacgtattgc ccggctatca
    28561 ttacacatac tacactatgg agcactttaa aaaattgctc ccacacgctg acttattttt
    28621 tattatggga gccgatttac tgcaagatat tggtgagggc aagtggaaga aagcagatga
    28681 acttataagt gaaaaccagt ttatcatcat ggcaagagag ggaatagaca tgttgaaagc
    28741 tattagtcat t
//
