LOCUS       NZ_CP064275            17417 bp    DNA     circular CON 17-APR-2024
DEFINITION  Priestia megaterium strain B1 chromosome, complete genome.
ACCESSION   NZ_CP064275
VERSION     NZ_CP064275.1
KEYWORDS    RefSeq.
SOURCE      Priestia megaterium (Bacillus megaterium)
  ORGANISM  Priestia megaterium
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 5078588)
  AUTHORS   Wang,C., Jia,Z., Zhao,J., Wang,X., Liu,Z. and Ji,F.
  TITLE     Comparative Genome Analysis of key enzyme genes in ammonia nitrogen
            Metabolism Pathway of bacteria from different sources
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5078588)
  AUTHORS   Wang,C., Jia,Z., Zhao,J., Wang,X., Liu,Z. and Ji,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2020) Guangdong Key Laboratory of Animal
            Conservation and Resource Utilization, Guangdong Public Laboratory
            of Wild Animal Conservation and Utilization, Institute of Zoology,
            Guangdong Academy of Science, Microbial Pathogenic Ecology Group,
            105 Xingang West Road, Guangzhou 510260, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. V1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 252.67x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022811885.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 10:12:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,571
            CDSs (total)                      :: 5,383
            Genes (coding)                    :: 5,338
            CDSs (with protein)               :: 5,338
            Genes (RNA)                       :: 188
            rRNAs                             :: 15, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 137
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 12 of 46
            Pseudo Genes (incomplete)         :: 26 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:16:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2573851
            Orig. end                         :: 2591268
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP064275.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..17417
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="phosphonate"
                     /region_number="5"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     cand_cluster    1..17417
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..17417
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:12417]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..12417
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     gene            complement(40..714)
                     /locus_tag="IQ966_RS13660"
     CDS             complement(40..714)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081817.1"
                     /locus_tag="IQ966_RS13660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_226556132.1"
                     /transl_table=11
                     /translation="MTLKFIHGNYLKYNKGTGFISCHVDALHAEPPAADLFTDKIVENL
                     VKKTGCAGIVSTVSRTTADLNRNLNETNYEGIQEYRRTIQEIVKSLKIVDSNNCLLTPY
                     LHLSFHGMKDTHYGPYAIEVGTLYGQSCSKEVKDWFQQALTKKAEDSFPKIHVIFDNKF
                     VGNESIAFHRQGDNKNYTGYSHNFNTFQIELSRTLRSKHRTTIINIFSEIIDDFQQTFV
                     AT"
     gene            960..3581
                     /locus_tag="IQ966_RS13665"
     CDS             960..3581
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081818.1"
                     /locus_tag="IQ966_RS13665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YheC/YheD family protein"
                     /protein_id="WP_243548784.1"
                     /transl_table=11
                     /translation="MDPMREELGILSDKEMTLQTLNLNNIPSVELVDPKTCSYPVIGRK
                     YGHYSGRDIVIVNTKDQAIYEGYDYFTKIYAIDKEYCLEVEGLSVKKVQVVTSEHVVFN
                     EIPIRTQAFGWKLEQINSMDVPEMLTNVAIRALYVTGAKSGFVKMGVLENGECIVTDIN
                     SSESEWIENPLKPSLPFSMGADVEFMLSCDGELLPASTFFSVEGPVGCDERQIEQDSGE
                     YALVEVRPEKANSSTELFENIQKLIEKASAQVPYENVHFRAGSMPFSGYQCGGHIHFGI
                     PLSLSLLRALDHYLAIPVALIEESKTAKLRRKTNHGGLGRYREKPYGFEYLTLSSWIID
                     PRITLSTLALAQLVATHHHELKSEFLFHPLTQRAYYQGNKIFLKRMWKDIKANLMKTSS
                     YSYYQNELSFLFEMIEKEIPCDESNDIRRNWNAKISKEIYDRGHIIQIPKKLRLKYGLQ
                     EGQSTIISAGKAISTATVHSYPFSFRHPNMVQLSKSLRDKLSLPKDWCPKLSASEGIIT
                     LGPIIGILANRPFERQTTYFHHLCRLANEKRMLVYVFEPEDIDWEKKLVKGTTINGEGL
                     FPFPAVIYDRYFIDGRKNILIDEVRAKLQAIYKIPFVNSSNLFQLTGDKWATYELLMKE
                     YEEFLPESRLVQSPKDIAEMLDRYGEVYLKPLGGALSKGVMRIVRRPTGIFWFDLNKKE
                     LHQFSNMEELFALLSPLMKNNPYLVQEGIRRKQHKDKNLEIRVYMQKNEKQIWLRTGMV
                     ARLTGEDVLTEDSETNMRLSKILNSLYPDPTDRRLIINQLAKISKNIVATVEEKVGPFG
                     ELAVDLCIDQYGSIKLLEINAKPDSLFSQIRAYKLRTLAGIRLLNYASSLAGYEEEKED
                     LT"
     gene            3578..4987
                     /gene="murF"
                     /locus_tag="IQ966_RS13670"
     CDS             3578..4987
                     /EC_number="6.3.2.10"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="murF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055491.1"
                     /locus_tag="IQ966_RS13670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="WP_014461510.1"
                     /transl_table=11
                     /translation="MKHFTLNEILKQTGGDLCYGSGNPVIKHAINYAKKDIEDHTLIFH
                     LDREPIRGKYWKENHSIVVITDHPELCTNLEGDIILVKTAQLEEAYWKFIEYYRGLFDL
                     PVIGVTGTCGKTTTKEMIRQILIEDYKVKATWMSMNSMSVNLRYLTGIDDTTEVAVFEM
                     PVAYPGYLKVACRYFQPQIRILLNIGVHHLADCETPEEYMKAKAEIVEGLDPNSGILIL
                     NADDENIKRVLDVKHLQNVVYIGKNNDSQFQAKDIKYANGGMTFTLKHKNKEYDAFIPG
                     YGEHNVYNALAAIAAVSYVGVDISVAIQRLSLFEQVEEHLEFKRGMNECTVINDTWNSS
                     PLSMATALGVLSDMSKEKQSIALLGYMPQLGEGPYAIKQYEDMGKKAAESKIDILVVVG
                     DEAKAIGTGALKYGMDPNRVYFCKTGDEVYNVLAPYLNENTIILLKVTHRVMTKPTFKE
                     LRNKLILDNEE"
     gene            5001..6518
                     /gene="aepX"
                     /locus_tag="IQ966_RS13675"
     CDS             5001..6518
                     /EC_number="5.4.2.9"
                     /GO_function="GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA]"
                     /GO_process="GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="aepX"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) phosphonates-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231: carboxyvinyl-carboxyphosphonate"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055492.1"
                     /locus_tag="IQ966_RS13675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="WP_014461509.1"
                     /sec_met_domain="phosphonates-like (E-value: 1.9e-137,
                     bitscore: 455.0, seeds: 13, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 4.7e-126, bitscore:
                     418.0, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 7.2e-63, bitscore:
                     210.5, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKTKQLRELIESKQLEFIMEAHSALSAKIVEEAGFKGIWASGLS
                     LSAALGVRDNNEASWTQVLDILEFMSDATSVPILLDGDTGYGNFNNARRLVKKLEQRDI
                     PGVCIEDKLFPKTNSFIKGEAQPLADINEFCGKIKAMKDSQNDEDFVVVSRVEAFIAGW
                     GLKEALKRAEAYRQAGTDAVLIHSKRSDMTEIEAFMKEWGNRLPVIIVPTKYYSVPTDK
                     FSDLGISLSIWANHNLRSSITAMQKTSAQIYYDKSLINVEKNVSPLEEVFRLQKAEELD
                     MAEKLYLSSANHSINAMILVKKSANDVLITEQMNQLKKVGVTNSIKVDSNKAMEDKESP
                     NQGELYDLYAARDKLGTATLISDSNIVYKTHVFEELIHESGDITIVAGADYDLKGDQTS
                     YVTAEFPYSKKLYSKTVDLIQMSSSPNSKNIHGEFIGLWKVSGKGTEVVKKVLEELAQR
                     EDFAQLTFSDFFNYINLIHPVAVKYTMGSWIDTETYIKLQKDGGKND"
     gene            6511..7641
                     /gene="aepY"
                     /locus_tag="IQ966_RS13680"
     CDS             6511..7641
                     /EC_number="4.1.1.82"
                     /GO_function="GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="aepY"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081821.1"
                     /locus_tag="IQ966_RS13680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="WP_226556131.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 4.4e-09, bitscore:
                     34.4, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.6e-13, bitscore:
                     48.9, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISTSSFGNQLKKLGFQFYSGVPCSYLKPLINYAINECEYVAAAN
                     EGEAVAIAAGASIAGKKAVVLMQNSGLANAVSPLTSLIHPFKIPVLGFVSFRGEPGFPD
                     EPQHELMGEITPALLNTMKIKWEYLSFDFTEVKNQLRRANQAIENNESYFFIVKKGTFE
                     SVKLSEKSMKNVTPKMKSEQITTDELPSRYEALQVVNSLKDNDTIQLAATGKTGRELYE
                     IEDAKNNFYMVGSMGCVSSIGLGLALSRNEKNIITIDGDGALLMRMGNLATNGYYCPEN
                     LLHILLDNQTHDSTGGQHTVSHNINFVDIASSCGYQNSIHVHNLNELEMYIKQWKNDKK
                     LTFLYLKIAKGSKKNLGRPEMKPYEVRERLQVFLDD"
     gene            7634..9475
                     /locus_tag="IQ966_RS13685"
     CDS             7634..9475
                     /EC_number="2.6.1.37"
                     /GO_function="GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_5 (266-541). E-value: 3.5e-30.
                     Score: 97.0. Matches aSDomain:
                     nrpspksdomains_IQ966_RS13685_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055494.1"
                     /locus_tag="IQ966_RS13685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="2-aminoethylphosphonate aminotransferase"
                     /protein_id="WP_014461507.1"
                     /sec_met_domain="NTP_transf_3 (E-value: 1.4e-10, bitscore:
                     40.0, seeds: 297, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_5 (E-value: 1.9e-24, bitscore:
                     84.6, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKTAVILAAGMGSRIRKRAGNRPKGFLMIDEKSIIEHSISKLIE
                     AGVKTIFIGTGYMKEEYEKLMVRYPQIKCVYNSRYETTGSLFTLYQFKSYIKEDFLLLE
                     SDVIYEKSALKTLINHSRSDVILASKLNDAGDEVYIEADENNNLKNMSKQKEELNSIYA
                     ELVGLTKLSYATFQRLCDEANTLFQLNQAIDYEYGLVKISEKANVYVHKLDNLAWCEVD
                     DEDHWARATTIVYPIIKAREGIPHAVKRNILLNPGPATTTDTVKYAQIVEDICPREKEF
                     GETMEFISAELTKFVGNSDKYTTVLFGGSGTAAVESILSSVIDNGTVVIINNGPYGERM
                     CKIAEIYGLDYAEYKSSAERAIDMNDLEVFIKKISANVSYLALVHCETSTGLLNDIEQI
                     GKLCAVENIEMIVDAMSSYAAVPIDMQKMNISYLAASANKNLQGMAGVSFVIANKERLE
                     KTKKMKPRNLYLHLYDQYRYFQMNKQMRFTPPVQTMYALKQAIIETQWEGIERRYERYS
                     KSWTTLTDGITRLGLTYLVPETHHSKIITSILEPSYQNYNFDIMHDFFYKQGFTIYPGK
                     IDKLETFRIANIGNITYRDIERFLHLLEQYLNTLKGE"
     aSDomain        8432..9256
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IQ966_RS13685_Aminotran_5.1"
                     /evalue="3.50E-30"
                     /label="IQ966_RS13685_Aminotran_5.1"
                     /locus_tag="IQ966_RS13685"
                     /protein_end="541"
                     /protein_start="266"
                     /score="97.0"
                     /tool="antismash"
                     /translation="YAQIVEDICPREKEFGETMEFISAELTKFVGNSDKYTTVLFGGSG
                     TAAVESILSSVIDNGTVVIINNGPYGERMCKIAEIYGLDYAEYKSSAERAIDMNDLEVF
                     IKKISANVSYLALVHCETSTGLLNDIEQIGKLCAVENIEMIVDAMSSYAAVPIDMQKMN
                     ISYLAASANKNLQGMAGVSFVIANKERLEKTKKMKPRNLYLHLYDQYRYFQMNKQMRFT
                     PPVQTMYALKQAIIETQWEGIERRYERYSKSWTTLTDGITRLGLTYLVPETHH"
     gene            9481..9903
                     /locus_tag="IQ966_RS13690"
     CDS             9481..9903
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461506.1"
                     /locus_tag="IQ966_RS13690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NUDIX hydrolase"
                     /protein_id="WP_116742051.1"
                     /transl_table=11
                     /translation="MNQKLAVAVMVVNQDHHVLLVKNHRRGWEFPGGFVEAGESITDAG
                     IREVKEESGIVIEVTNFLGVEQDVKRSTTVILLKGKAISGEISVSSETQDVGYFTFEEA
                     KGIIKLDNFKDRMTRCLNETEIPFFYVLKDTDSFYV"
     gene            complement(10011..10724)
                     /gene="budA"
                     /locus_tag="IQ966_RS13695"
     CDS             complement(10011..10724)
                     /EC_number="4.1.1.5"
                     /GO_function="GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006113 - fermentation [Evidence IEA]"
                     /GO_process="GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="budA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215002.1"
                     /locus_tag="IQ966_RS13695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acetolactate decarboxylase"
                     /protein_id="WP_182421369.1"
                     /transl_table=11
                     /translation="MSNEEKLTIYQTSTMIALLDGVFDGVVTYKDLAKKGDFGIGTFNQ
                     LDGEMIAFDGEFYHIADGKAAPISEDAKTPFAIMTTFYEDISYRVEKEMNREQLETLMN
                     KLIPSPNLFYAVRIDGKFKEVKTRTVAKQEKPYPSMVDAAADQPTFTSSDVNGTLAGFY
                     TPEYSSGIGVPGYHLHFIDEYKKEGGHVLDVSVQDVTIQICKKTKLNLNLSETSEFLNA
                     NLEDHDVEEEIATTE"
     gene            complement(10741..12417)
                     /gene="alsS"
                     /locus_tag="IQ966_RS13700"
     CDS             complement(10741..12417)
                     /EC_number="2.2.1.6"
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0003984 - acetolactate synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="alsS"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461504.1"
                     /locus_tag="IQ966_RS13700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acetolactate synthase AlsS"
                     /protein_id="WP_243548785.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 4.2e-51, bitscore:
                     171.2, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.9e-43, bitscore:
                     145.6, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNADTTETKKGADLLVESLASQNVPYIFGIPGAKIDGVFDVLKE
                     KGPEVIVCRHEQNAAFMAAAMGRLTGTPGVCLVTSGPGAANLATGLVTATSEGDPVVAL
                     AGAVPRADRLKKTHQSMDNEGLFKSIAKFSVEVEDAANVPEVITDAFREAVSSPSGASF
                     VSLPQDVMDSETSASTLSVLKTPTIGAANLDAVAQAIHTIRIAKLPVLLLGMRASKSKA
                     VESIRQLIEEHDLPVVETFQGAGAVLRELETHFFGRVGLFRNQPGDVLLKHADVVLTIG
                     YDPIEYPPSLWNSESQRSIIHLDDVQAQADHHYQPEIELVGDIPSTIDILTDKLPHLIR
                     SKESASFLKTLKSKLEQPDDQYEFSNDTHLHPLQLIEILQNFVEDDTTITCDVGSHYIW
                     MARYFRSYEPQHLLFSNGMQTLGVALPWAISAALVRPHKKVLSMSGDGGFLFSAMELET
                     AVRLNLPIVHIIWNDQTYDMVGFQQQIKYGRKSAVEFGSVDFVTLAESFGAKGFRVNSP
                     EEFSDTLASAFREQCPVIIDVPVDYKDNEKLNTQLLPDQIN"
     misc_feature    complement(12517..12531)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.86"
                     /tool="antismash"
     gene            complement(12647..12811)
                     /locus_tag="IQ966_RS13705"
     CDS             complement(12647..12811)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461503.1"
                     /locus_tag="IQ966_RS13705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="WP_014461503.1"
                     /transl_table=11
                     /translation="MKQPQLEKEIRALQSDIYQLAKKTSSYSHGEILKLSQKLDQKIVS
                     YQKLFNHTK"
     gene            complement(13674..13871)
                     /locus_tag="IQ966_RS13710"
     CDS             complement(13674..13871)
                     /GO_component="GO:0005575 - cellular_component [Evidence
                     IEA]"
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /GO_process="GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055498.1"
                     /locus_tag="IQ966_RS13710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YwbE family protein"
                     /protein_id="WP_014461501.1"
                     /transl_table=11
                     /translation="MNGQNRKDIAPGTKVDIVLKADQRSGKLTHGTVKDILTNSSFHPH
                     GIKVRLTDGQVGRIKEIYPN"
     gene            complement(13871..14050)
                     /locus_tag="IQ966_RS13715"
     CDS             complement(13871..14050)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002015431.1"
                     /locus_tag="IQ966_RS13715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_014461500.1"
                     /transl_table=11
                     /translation="MTEQLKKKISLQDAIKQQLANKKNQSSNKAKNGSTNQATKKLTSQ
                     QTKKTNNQRKRTGV"
     misc_feature    14112..14127
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.77"
                     /tool="antismash"
     gene            complement(14134..14502)
                     /locus_tag="IQ966_RS13720"
     CDS             complement(14134..14502)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461499.1"
                     /locus_tag="IQ966_RS13720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_014461499.1"
                     /transl_table=11
                     /translation="MKKYNIPVEAALEVIGGKWKVVILCHLIKGTKRPGELKRIMPRVT
                     QKVLTQQLRELEADGIIVRKIYNEMPPRVEYSLTDYGWSLKSILDSLCSWGEHHIEKTY
                     PDKEAVLMQPVEEVTVSN"
     gene            14832..16031
                     /locus_tag="IQ966_RS13725"
     CDS             14832..16031
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1184: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461498.1"
                     /locus_tag="IQ966_RS13725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_014461498.1"
                     /transl_table=11
                     /translation="MNTLSQTQQSKKSGALPLLALAISAFGIGTTEFVPVGLLSSLADD
                     LNISITLAGLLISGYAMGVAFGAPVLTALTSKMSRKSLLMLLMVIFIVGNSVAALSTSF
                     GLLLIARIITAFSHGIFFSIGATIAADLVPEDKRASAIALMFTGLTVATVTGVPLGTFI
                     GQAFGWRATFWGVALLGVIAIIASALLVPKNLKEAPPAKFSDQLKILKNGPLLLSFAIT
                     ALGYGGTFVAYTYLAPILEKLTGYAPSAVSILLLVYGVAVAIGNTIGGKAANKNPLKAL
                     FWMFLIQAVILVILSFTASFKVAGTITIFAMGLLAFMNVPGLQVLVVNLAEKYVPSAVN
                     VASALNIAAFNVGIAIGSFVGGLIVDSIGLVHTPWIGGVMVLGAVLLTAWSRSIEAKAK
                     "
     gene            16089..17045
                     /locus_tag="IQ966_RS13730"
     CDS             16089..17045
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081827.1"
                     /locus_tag="IQ966_RS13730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LLM class oxidoreductase"
                     /protein_id="WP_208732719.1"
                     /transl_table=11
                     /translation="MTTFQDHKAYRRMYKENELTLGLHMPLENYQYATPTMEKQVELTQ
                     LAEQYGFTSLWFRDVLLQDPSFGDPATGQIYDMLIYLTYLASQTKEIALSTSAIVLPLR
                     HPLRVAKEMATIDQLFPERLIMGVSSGDRQADFRGLGIPHEERGKRFVEAFHYLEEALN
                     KEFPSIQSALGSIQGANVVPKPTKRIPTMITGYSQQNMEWFAQYGDGWMYYPRHPHFQA
                     EAITQWRKLVDSYHPGVFKPFTQPMHLDLAEDPNEMPTPIRLGFRAGRNTLIELLSIYK
                     EIGVNHLFFALFDSKRPAEEVIHELGEEVLPHFPGHM"
ORIGIN
        1 tatataaaag aaatagtaga cggaaaagat tcctaaaaac taagtagcga cgaatgtttg
       61 ttgaaagtca tcaataatct cggaaaatat atttattatg gtagttctgt gtttgctgcg
      121 cagagttcgg gataattcaa tttgaaacgt attaaaatta tgactgtaac ctgtatagtt
      181 cttattgtca ccttgtctgt gaaaggcaat tgattcgttt ccaacaaatt tattatcaaa
      241 tattacatgg attttaggga acgaatcttc agctttttta gtcagggcct gttgaaacca
      301 atctttaact tcctttgagc aggattgtcc atataaagtc cccacttcaa ttgcataagg
      361 cccgtaatgt gtatccttca ttccatgaaa agataaatgc aggtagggtg tcagaaggca
      421 attattacta tctactatct ttaaagattt aacaatttct tggattgttc ttctatattc
      481 ttgtattccc tcatagtttg tttcatttag atttctattt aaatctgctg tggttctaga
      541 aaccgtacta acgattcctg cacatcctgt tttcttgact aaattctcta caattttatc
      601 cgtaaataaa tcagcagcgg gtggttctgc atgaagtgca tccacatgac agcttataaa
      661 gccagttcct ttattgtatt ttaagtagtt tccatgaatg aattttaatg tcatttacta
      721 tttctccttt tatatcgttt gaaatctatc ctttctataa taaatgcagt aaaaataaaa
      781 tcggtgaatt attacagaag ttaaatacat acataaaaag ttttcggagg tggtggaaat
      841 aaatagatat atgtacagga gtattttgta tagttacata ctatatgagt agctgattgt
      901 tgttataggg cattaaagga gcaaaatacg atagaaagtt ctaactagga ggctttaaaa
      961 tggatccaat gagagaagag ttagggatat tatctgataa agaaatgacg ttgcaaactc
     1021 ttaatctgaa taatataccg tctgttgaat tagttgaccc aaaaacctgt tcatatccag
     1081 tgataggtag gaaatatggt cactatagcg gaagagatat tgttatcgtc aatacaaagg
     1141 accaagcaat atatgaagga tatgattatt ttacgaaaat atatgcaata gacaaagaat
     1201 attgtctaga agtagaagga ctgagcgtga aaaaagtaca agtagtaacc agtgaacatg
     1261 ttgtatttaa tgaaataccc attcgaacgc aagccttcgg ttggaaatta gagcagataa
     1321 acagcatgga tgtaccggaa atgctgacga acgtagccat tcgagcgctc tacgtaacgg
     1381 gagctaaaag cggctttgtc aaaatggggg tattagaaaa tggagagtgt atcgtcaccg
     1441 atattaactc atcagagagt gaatggatag aaaatccatt aaagcctagt cttcctttta
     1501 gcatgggtgc tgatgttgag tttatgttaa gttgtgatgg agagcttcta ccagcttcta
     1561 cttttttttc tgttgaaggt ccggtaggct gtgatgaaag acaaatagag caagatagcg
     1621 gtgagtatgc attagtagaa gttaggcctg aaaaagcgaa ttcatcaaca gagctatttg
     1681 agaatatcca aaaattaata gaaaaagcat ctgcacaggt accatatgaa aatgttcatt
     1741 ttcgagcagg cagcatgccg ttttcagggt atcaatgtgg aggacatatt cattttggca
     1801 ttccgctttc tttatcctta ttaagagctc ttgaccacta ccttgctatt cccgtagcgc
     1861 tgatagaaga atctaagacc gctaaactca gacgaaaaac gaaccatgga ggtcttggac
     1921 gctatcgtga gaaaccgtat ggatttgagt atttaacttt aagctcttgg attatcgacc
     1981 ctagaattac tctctccact ttagctcttg ctcagctagt tgctacccat catcatgaat
     2041 taaaaagcga atttttattt catccactta cgcagcgtgc ctactatcaa gggaataaaa
     2101 tctttcttaa acgaatgtgg aaagatatca aggccaatct tatgaaaaca tccagctatt
     2161 catactatca aaatgaacta tcttttctat ttgaaatgat cgaaaaagaa attccttgtg
     2221 atgaatcaaa tgatattcgc aggaattgga atgcaaagat ttctaaagaa atctatgacc
     2281 gcggacatat tatccaaatc cccaaaaagc tccgtttaaa atacggcctc caagaggggc
     2341 agtctacgat tattagtgca ggaaaggcaa tatcaacggc aactgtgcat tcttatcctt
     2401 tttcatttcg ccatccaaat atggttcaac tctctaaatc tctccgtgat aaactatctc
     2461 tcccaaaaga ttggtgcccc aagctatcag cttcagaagg cattataact ttaggtccta
     2521 tcattggcat tcttgctaac cgtccttttg aaagacaaac aacttatttc caccatctat
     2581 gtcgcttagc aaatgaaaag cgaatgcttg tatatgtgtt tgaacccgaa gatattgatt
     2641 gggagaaaaa attggttaaa ggaacaacca ttaacggtga agggctcttt ccttttcctg
     2701 ctgttattta tgaccgttat ttcatagatg gcagaaaaaa tattttaata gatgaagtaa
     2761 gagcaaaact tcaggcgatt tataaaattc cttttgttaa ttcttcaaac ttgtttcagc
     2821 ttacgggaga taagtgggca acatatgaac ttcttatgaa agaatatgaa gaatttcttc
     2881 cagagtcacg tctcgtacaa agtcctaaag acattgcaga aatgcttgat aggtacggag
     2941 aagtttattt aaaaccgctt ggcggggcct taagtaaagg agtcatgcgc atcgttcgcc
     3001 gaccaacagg catcttttgg tttgatctta ataaaaaaga gctacatcaa ttcagtaata
     3061 tggaagaatt atttgctttg ctctcccctt tgatgaaaaa caatccttat cttgttcaag
     3121 aaggaattcg cagaaaacag cataaagata aaaatttaga aatacgggtt tatatgcaaa
     3181 aaaatgaaaa gcaaatttgg cttcgaacgg gcatggttgc gcgtctcacc ggtgaagatg
     3241 tgctaacgga agattcagaa acgaatatgc gtctcagtaa aatattgaat agtttatatc
     3301 ccgatccaac agacaggagg ctcattataa accaattagc taaaatatct aaaaatattg
     3361 tggcaacagt agaagagaaa gtgggtcctt ttggagagct agcggtagat ttatgtatag
     3421 atcagtacgg ttccataaag ttgcttgaaa taaatgcaaa accagatagt ctgttctcac
     3481 aaattagagc ttataagctg cgtactttgg cggggattcg tctgcttaat tatgcgtctt
     3541 cactggctgg ttacgaagaa gaaaaggagg atttaacatg aagcacttta cattaaatga
     3601 aatccttaaa caaacggggg gagacctttg ttatggatca ggaaatcctg ttattaaaca
     3661 tgctataaac tacgctaaaa aggatataga ggatcataca ctcatctttc atttagatag
     3721 agaaccgatt agaggaaagt attggaaaga aaatcattca attgtcgtta taacagatca
     3781 ccctgagctg tgcacgaact tagagggaga tattattctt gttaaaacag ctcaattaga
     3841 agaggcttat tggaaattta tcgaatacta ccgagggctt tttgatcttc ctgttattgg
     3901 agttacagga acttgtggaa aaacgaccac gaaagagatg atccgacaaa tattaattga
     3961 agactataaa gtgaaagcta cgtggatgag tatgaattct atgtctgtaa atctacgtta
     4021 tttaacaggt attgatgata cgacagaagt agctgttttt gaaatgccgg tcgcttatcc
     4081 aggttattta aaagttgcgt gccgctactt tcagccacaa atacgaattc tattaaatat
     4141 aggcgtgcac catcttgctg attgtgaaac acctgaagaa tatatgaaag caaaagccga
     4201 aattgtagaa ggcttagatc caaatagcgg aattttaatt ttaaacgcag atgacgaaaa
     4261 tattaagaga gtgcttgatg taaagcatct tcaaaatgtt gtatatatag gtaagaataa
     4321 tgattcccaa tttcaagcaa aagatattaa atatgctaat gggggaatga cttttacttt
     4381 aaagcacaaa aacaaagaat acgatgcttt tatccctgga tatggtgaac ataatgtgta
     4441 taatgcactg gctgccattg ctgcagtatc ttatgtgggc gttgatattt ccgttgccat
     4501 acaaagacta agcttatttg aacaagtaga agaacatttg gaatttaaaa gaggaatgaa
     4561 tgaatgtacg gtaattaacg acacatggaa ctcttcacct ttatcgatgg ctacagctct
     4621 tggagttctc agtgatatgt caaaagagaa acaatctatc gctttattag gctacatgcc
     4681 tcagctagga gaaggacctt atgcaattaa acaatatgaa gatatgggaa aaaaagcagc
     4741 ggaatccaaa attgacattc tagttgtagt gggagatgaa gcaaaagcta ttggaacagg
     4801 agctttaaaa tatggaatgg accctaatag agtctatttt tgtaaaactg gtgatgaagt
     4861 atacaatgtg ctagctccat atttaaatga aaatacaata attttattaa aagtaacaca
     4921 tcgtgttatg acaaaaccta cttttaaaga attaagaaat aaacttattc tagacaacga
     4981 agagtaaaag gaggatagac atgaaaaaga ccaaacagct aagagagttg atagaaagca
     5041 aacagctaga gtttattatg gaggctcaca gtgcgctatc tgcaaagatt gtagaagaag
     5101 cagggttcaa agggatatgg gccagcggcc tgtctttatc cgcagcactg ggtgtaagag
     5161 ataataatga ggcttcgtgg acacaggtat tagatatctt agagtttatg agtgatgcaa
     5221 cgtccgttcc cattcttctg gatggtgata caggctatgg gaattttaat aatgcaagaa
     5281 gattagttaa gaagttggag cagcgagaca ttccaggcgt atgtattgaa gataaattgt
     5341 ttccaaaaac aaactcattt attaaaggtg aagcacagcc gctcgcagat attaatgaat
     5401 tttgcggcaa aattaaagca atgaaagata gccaaaatga cgaagatttt gtagtggtat
     5461 cgcgtgtaga agcatttatt gcagggtggg ggctgaaaga agctttaaaa cgtgcagaag
     5521 cttatcgcca agcgggaacc gatgccgttt tgattcatag taaaaggtca gatatgacag
     5581 agattgaagc ttttatgaaa gagtggggaa acaggcttcc ggttattatt gttccaacca
     5641 aatattattc ggttccaact gataaattta gtgatttagg aattagctta agcatttggg
     5701 ccaaccataa tttaagatct tctattacag ctatgcagaa aacgtccgct cagatttatt
     5761 acgataaaag tttgattaac gtagaaaaaa atgtctctcc gcttgaggaa gttttccgtc
     5821 ttcaaaaagc tgaagagctc gatatggcag agaaacttta tttaagctct gccaatcata
     5881 gtattaacgc tatgattctg gtaaaaaaat cagcaaatga cgttttaata actgagcaaa
     5941 tgaatcagtt aaaaaaagtt ggcgtgacaa atagtataaa agtagacagt aacaaagcta
     6001 tggaagacaa ggagtctcct aatcaaggag aattatatga tctttatgct gcaagagata
     6061 agctaggaac ggctacttta atcagtgata gtaacatcgt gtataaaact catgtttttg
     6121 aggagttaat tcatgaaagt ggcgatatca ctatcgtagc aggagctgat tatgatttaa
     6181 aaggggatca aacatcttat gtaacggctg aatttcccta ttccaaaaag ctttattcca
     6241 aaacagtaga cttaattcaa atgtcatcca gtcccaattc taaaaacatt catggtgaat
     6301 tcattggtct ttggaaagtt tcgggaaaag gcactgaggt tgtaaaaaaa gttttggaag
     6361 aactggcaca aagagaagat tttgctcagc tcacgttctc tgattttttt aattatatta
     6421 atttaattca tccggttgct gttaaatata cgatgggatc atggatagat accgaaacgt
     6481 atataaagct tcaaaaagat ggtgggaaaa atgattagta catcatcatt tgggaatcaa
     6541 ttaaaaaaac taggttttca attttatagt ggggttcctt gttcctatct taaacctcta
     6601 attaattacg ctatcaacga atgtgaatac gttgcagcag caaatgaagg agaagcagta
     6661 gcaattgctg ctggcgcatc aattgctggc aagaaagcgg ttgttttaat gcagaactcc
     6721 ggattagcaa atgctgtttc ccctttaacc tctcttattc atccgtttaa aattcctgtc
     6781 cttggatttg tcagttttcg cggagaacct ggatttccgg atgaaccgca gcatgaatta
     6841 atgggagaaa ttactcctgc tctgctaaat acaatgaaaa taaaatggga atatttatct
     6901 tttgatttta ccgaagttaa aaaccaatta cggcgggcta atcaagctat tgaaaataat
     6961 gaaagctact tctttattgt gaaaaaagga acgtttgagt cagttaagct ttcagaaaaa
     7021 tcaatgaaaa acgttactcc taaaatgaaa agtgaacaga taacaaccga cgagttacct
     7081 tcaaggtatg aggcactaca agtagttaat tcactaaagg acaatgatac cattcaattg
     7141 gccgctacag gaaaaacggg aagagagctt tacgaaatag aagacgcaaa aaataatttt
     7201 tatatggttg gatctatggg gtgcgttagt tccattggtt tgggcttagc tttgagcaga
     7261 aatgaaaaga acatcattac gattgatgga gacggagctt tgcttatgcg catgggaaat
     7321 ctagcaacca acggatacta ttgtccggaa aatttattac acattctttt agacaatcaa
     7381 acccatgatt ctacaggggg acaacatacg gtctctcata acattaattt tgtagatatt
     7441 gcttcttcct gtgggtatca aaattccatt catgttcata acttaaatga gttagagatg
     7501 tatatcaaac agtggaaaaa tgataaaaaa ttaacatttt tatatcttaa aatagcgaag
     7561 ggatctaaaa agaacctggg gcgtcctgaa atgaagcctt atgaagtcag agaaagactg
     7621 caggtgtttt tagatgatta agacagctgt tattctagct gcggggatgg ggagcaggat
     7681 tcgtaaaaga gctggaaacc gccctaaagg ttttttgatg attgatgaaa aatcgattat
     7741 tgaacattcc atttcaaagt taatagaagc aggagttaag acgattttta ttggaacagg
     7801 ttatatgaaa gaagaatatg aaaagctaat ggttagatac cctcaaataa aatgtgttta
     7861 taattctaga tacgaaacaa cgggaagtct gtttacgctg tatcaattta aaagttatat
     7921 taaagaagat tttttactat tggaatcaga tgtaatttat gaaaagagtg cgttgaaaac
     7981 attaataaat cattcgagat cagatgttat actagcgagt aaattgaacg atgctggcga
     8041 tgaagtgtat atcgaagctg atgaaaataa taaccttaag aatatgtcaa aacaaaaaga
     8101 agagttgaat agtatttatg cagaactagt tggtttaacc aaactctctt atgctacatt
     8161 tcagagactt tgtgatgaag caaatacgct gtttcagcta aatcaagcga ttgattatga
     8221 atacgggctt gttaaaattt ccgaaaaagc aaatgtgtat gttcataaac tggataattt
     8281 ggcatggtgt gaagtggatg atgaagatca ctgggcaaga gcaactacta ttgtttatcc
     8341 gattattaaa gctcgtgaag gtattccgca cgccgttaaa agaaatatcc tcctgaatcc
     8401 tggaccggca actacaacag acaccgtaaa atatgctcaa atagtggaag acatttgccc
     8461 gagagaaaaa gaatttggag agacgatgga gtttatctct gctgaattaa caaagtttgt
     8521 tgggaattca gataaatata ctacggtact attcggcgga tctggaacgg cggccgtgga
     8581 atcgatttta agttcagtaa ttgataacgg tacggttgtt attattaata atggtccata
     8641 cggtgaaaga atgtgtaaaa tcgctgaaat ttatgggctg gattatgcag aatataaaag
     8701 ctccgctgag cgggctattg atatgaatga cttagaagtt tttataaaaa agatttcggc
     8761 gaacgtttct tatctggcgc ttgttcattg tgaaacgtca acagggcttc ttaatgatat
     8821 tgagcaaatt gggaaacttt gcgcagtgga aaatatcgag atgattgtag atgcaatgag
     8881 ttcctatgca gctgttccaa ttgacatgca aaaaatgaat ataagctatc tggccgcaag
     8941 tgcaaataaa aaccttcaag gaatggcagg cgtgtcgttt gttatagcaa ataaagaaag
     9001 gttagaaaaa acgaagaaaa tgaagcctcg caacctttat cttcatttat atgatcagta
     9061 tcgctatttc caaatgaata aacaaatgcg ttttactcct cctgttcaga ctatgtatgc
     9121 cttaaagcag gcaattatag aaactcagtg ggaaggaatc gagagacgat atgaacgcta
     9181 ttcaaaatca tggactactt taacggatgg aattactcgg cttgggttga cctatttggt
     9241 tcctgaaaca catcattcta aaattattac ctctatcctc gaaccttcct atcaaaatta
     9301 taattttgat atcatgcatg atttttttta taaacaagga tttactattt atcccggtaa
     9361 aatcgataaa ttagaaacgt ttcgaatagc caatattggt aatatcacgt atagagatat
     9421 agaaagattt cttcaccttc ttgaacaata tcttaacact ttaaaaggag agtgaaaaaa
     9481 gtgaatcaaa aattagctgt agcagttatg gtggtaaatc aagatcatca cgtgcttcta
     9541 gtcaaaaacc atagacgggg atgggaattt cccggtggtt ttgtcgaagc aggtgaatcg
     9601 ataacagatg cgggtatacg agaagttaaa gaagaatctg gaattgttat tgaagtgaca
     9661 aactttctcg gagtagaaca agatgttaaa cgatctacta ctgttatttt attaaaggga
     9721 aaagcgatta gcggggaaat ttcagtttct agtgaaacgc aggatgtagg atattttacg
     9781 ttcgaagaag caaaaggcat cattaaatta gataacttta aggatcgaat gactagatgt
     9841 ctaaatgaga ctgaaatacc ttttttctat gttttaaaag acacggattc tttttacgta
     9901 taggcggaag gtttctgtgt tgtgatagat atgcggggtg aactcgagtg aaaaaactct
     9961 catcgtcatg cgtaacgatg agagtttttt tacagtaaaa gtatcgttgc ttattcagtc
    10021 gtcgcaattt cttcttctac atcatggtct tctaaattag catttaaaaa ttcacttgtt
    10081 tccgataaat tgagatttaa tttcgttttt ttgcagattt gaattgtaac atcctgaacg
    10141 gagacgtcta aaacatgtcc accttctttt ttatattcat caatgaaatg caggtgatag
    10201 ccgggaacac cgatgcctga ggaatattct ggcgtgtaaa aacctgctag tgttccattt
    10261 acatcagaag atgtaaatgt aggctgatca gccgctgcat ctaccataga aggatagggc
    10321 ttctcttgct ttgcaaccgt gcgggttttt acttctttga acttcccgtc aatgcgaact
    10381 gcatagaata aattagggct tggaataagc ttgttcatta aggtttctag ttgttcacgg
    10441 ttcatttcct tttctacacg gtaagagata tcttcataaa aggttgtcat aatggcaaat
    10501 ggtgtttttg catcttcgct aataggggcg gctttcccat cagcaatatg ataaaattct
    10561 ccgtcaaagg caatcatttc accgtccaat tgattaaacg taccgatgcc gaaatcaccc
    10621 tttttagcta agtccttgta agtgacaaca ccgtcaaata ctccgtctaa taaagcgatc
    10681 atagtggaag tttggtaaat cgttaatttt tcttcattgc tcattcttta ctcagtcctt
    10741 tcagttaatt tgatctggca gtagctgtgt atttaatttt tcattatctt tataatctac
    10801 aggcacatcg atgataacgg ggcattgttc acgaaaagcg cttgctagag tgtccgaaaa
    10861 ttcttctggt gagttaactc taaatccttt ggcaccaaaa ctttcagcaa gcgtaacaaa
    10921 gtctacactg ccgaactcta ccgcagattt tcggccatat ttaatctgct gttgaaagcc
    10981 aaccatatcg taggtttgat cgttccagat aatgtgaaca attggtaagt ttaaccgaac
    11041 ggctgtctcg agctccatag ctgaaaataa aaaaccgccg tctccagaca tggacagaac
    11101 ttttttatgg gggcgaacga gtgcagctga aatagcccaa ggcaaagcaa cacctagcgt
    11161 ctgcatgccg ttgctgaata gtaaatgctg aggttcatag gaacgaaaat aacgagccat
    11221 ccatatgtaa tgcgaaccaa cgtcacatgt gatggtagtg tcatcttcaa caaagttctg
    11281 caaaatttca attaattgta ggggatgaag gtgagtgtca ttagaaaact cgtactgatc
    11341 gtcaggttgt tcaagtttgc tttttaatgt ttttaaaaaa gaagcacttt cttttgaacg
    11401 tattaaatgt gggagtttat ccgttaatat gtcaattgtg cttggaatat cccctactaa
    11461 ctcaatttcc ggctgataat ggtggtcagc ttgcgcttgt acatcatcta aatgaataat
    11521 gcttctttga ctttctgagt tccaaagaga aggagggtat tcaattgggt catatccgat
    11581 tgttaagacg acgtcagcat gcttcaataa gacatctcca ggctgattgc gaaaaagacc
    11641 tactcgtcca aaaaagtgag tttcaagctc tcgtaaaaca gctcccgcac cttggaacgt
    11701 ttccaccaca ggaaggtcat gttcttctat aagttgacgg attgattcca ctgctttaga
    11761 tttacttgct ctcataccta aaagtaaaac aggtagtttt gctatgcgaa tagtgtgtat
    11821 agcttgggca acagcgtcta aattagcagc gccaatcgta ggagttttta gaacggatag
    11881 agtggaagct gatgtttcgc tatccattac atcctgcggc aagctgacaa aagatgctcc
    11941 tgaaggagaa gaaacagctt cgcgaaaggc gtctgttatc acttctggaa cgtttgcagc
    12001 atcttctacc tcaacgctga atttggcgat agatttaaat agtccttcat tatccattga
    12061 ttgatgcgtc ttttttaatc gatctgctct tgggacggct cctgctaggg ctaccactgg
    12121 atcaccttca gaagtagcag taacaaggcc agtggctaga tttgcggcac ctggacctga
    12181 tgtgacaagg caaacgcccg gcgtacctgt cagtcttccc atagcagctg ccataaacgc
    12241 agcattttgt tcatggcggc aaacaattac ttctggtcct ttctctttca aaacatcaaa
    12301 tacgccatca attttggctc ctggaatgcc aaaaatataa ggcacattct gacttgccaa
    12361 actttctact agtaaatcgg cgcctttttt tgtttcagta gtatcggcat tactcatcgt
    12421 gcattctctc ctttatgttt tttaaataag ttttaaggtc tactgattga atacccaatt
    12481 tgtatgacaa ctaaccaaag gaaaggagaa ggttttaatt ttctaaataa aaaacttccc
    12541 tattaattag ggaagcagag atgtacctat tgcgaataaa acatgtctgt tttatttgta
    12601 ggtttatggt acttgaggga tagcaagtga aagtcctacg cggctcttat ttggtatggt
    12661 taaaaagctt ttggtaacta acaatttttt gatctagttt ttgacttaat tttaaaatct
    12721 caccgtgaga atagctgctt gttttcttag ctaattggta aatatctgat tgtaaggctc
    12781 gaatttcttt ttctaactga ggttgcttca ttgaaatcct ctccaagtca gaatacttct
    12841 atcttctatc ttttatctaa tagtatgaaa ttccttcata tttgtaaaat gttttttgtt
    12901 ttgaaaaata tgacataggt gaagagctac tgacaagctt tgtcgaacac tttttttacc
    12961 tgttttcaac aagtatgata tactgaggct aggaaaaatg ataatctgga gcgcacgttc
    13021 agttattctt aacaagcgaa tgaattctta gctttcacaa gtgtaacttg taaggtgaag
    13081 aaattcatca cataatctta ttagatctga accttgttag cccggatgag gatttgtcga
    13141 aaacgattca ctagaaaaaa ccttcgagac cgccaactcg gaggtttttt tatttttgtg
    13201 tatggaaatt taaaaaagac atagactaat aaagaaaaag gtcccgcacc cataatcggc
    13261 cgtagctgac tacaccttgt ccatttctgc aaggaagctg tctttttact atagggaagt
    13321 gccgccaaac acgattccct aaagacaatc agcacacgag ccttatggat gaagaacctt
    13381 ttagaggata cttatcggtg attacttatt ttactactgg aaaaatagca aaataagagc
    13441 gactatggct cctaaaagct ctacgtaagc cgttaggagc tgaacattct cggcatctag
    13501 gaaaaatgac ttgaggattc gtctcagact atccaccacc tttttcttcc ttgcgtgaat
    13561 tcccaaggga aagaaagggg acgacagtgc ttctttattt taacaaaagt aggaaataat
    13621 aagtagattt atttaaagtg taaattaaaa aagcatgtag aatacatgct tttttaattt
    13681 gggtaaattt cttttatacg tccgacttgt ccatctgtta aacggacttt gatgccgtgt
    13741 ggatgaaagc ttgaattggt cagaatgtct ttgaccgttc cgtgtgtaag ttttccagaa
    13801 cgctgatctg cttttaaaac aatgtcgact ttagttccag gagctatgtc ttttctattt
    13861 tgaccgttca ttatacacct gttctctttc gctgattatt cgtttttttg gtttgttgac
    13921 tcgtcagctt tttggttgct tgattggtgc tgccgttctt agctttattt gaagattgat
    13981 ttttcttatt agcaagctgc tgtttgatag catcttgaag actaattttc tttttaagtt
    14041 gttctgtcat aagggttcct cctgaatacg tacatcgttt aaaaaacaac gaaagtaagg
    14101 gaatgaaaaa gctgactatc tagtcagctt tttttaatta gaaacagtga cttcttctac
    14161 aggctgcata agtacagctt ctttatcagg gtacgttttt tcaatatgat gttctcccca
    14221 agagcataat gaatcaagaa tgctttttaa agaccatccg taatctgtta aagaatattc
    14281 tactcgaggc ggcatttcat tgtatatctt tctaacaatg atgccgtctg cttcaagttc
    14341 gcgaagctgc tgcgttaata ctttttgtgt aacgcgcggc ataattcttt ttaattctcc
    14401 agggcgcttt gtccctttaa ttaagtggca aagaataacg actttccact tgcctccaat
    14461 gacttctagt gccgcttcca ctggaatatt atattttttc acgttaacaa ctctccttgt
    14521 tctagagata acggttaaat gcatggtttc gcattttttc tgatgtttat cataccacta
    14581 tagcgcagaa ttttctaggt gtaagcattt ttttagaata cccaatagtt catgacttta
    14641 gtatttacgc ttattgtaac ctccaggtac ctatatcact tgaaagtgcg tactttttct
    14701 ttggttttac atcgctcata ataacaattg ttgatggcga gttagcgaat atatgaaagg
    14761 tacctaagct aatcagccgt aaaagaagct attataaaga agttattata atgaacagga
    14821 ggcgttaaga agtgaacaca ctttcacaaa cgcaacaaag taaaaaaagc ggtgctttgc
    14881 cgctattagc gctagctatt agcgcctttg gaatcgggac tacagagttt gttccagtag
    14941 gtttattatc atcgcttgca gatgatttaa atatttccat cacactagca gggttattaa
    15001 tttcaggata cgcaatgggg gttgcatttg gagctcccgt attaacagct ttaacaagta
    15061 aaatgagcag aaaatcactg ttgatgctat tgatggttat ttttatcgta ggaaactcag
    15121 tagcagcttt atcaacaagc tttggtctgc tgcttatagc acgaattatt actgcatttt
    15181 cacacgggat tttcttttct atcggtgcga ctatcgctgc tgatttggtg ccggaagata
    15241 agcgggcaag tgccattgct ttaatgttta caggtttgac agttgcaact gttacaggtg
    15301 ttccgcttgg tacatttatt ggacaagctt tcggttggag agcaacattt tggggagtag
    15361 ctttacttgg cgttattgct attattgcaa gtgctctttt agtacctaaa aacttaaaag
    15421 aagcgccgcc ggcaaagttt agtgatcaat tgaaaatttt aaaaaacggg ccgcttttac
    15481 tatcgtttgc tattactgct ttagggtatg ggggaacgtt tgtagcttat acgtatttag
    15541 cgcctattct tgaaaagcta acaggatacg caccaagcgc agttagtatc ttgctactgg
    15601 tatatggtgt agcggtagct atcggaaata cgatcggagg aaaagctgct aataaaaatc
    15661 cattaaaagc attgttttgg atgtttttaa ttcaagcagt tatcttagtt attttatcgt
    15721 ttaccgcgtc gtttaaagta gcgggaacaa tcacgatttt tgccatgggc ttattggcat
    15781 ttatgaacgt acctggactg caagtgctgg tcgtgaactt agctgaaaag tatgtccctt
    15841 cagcagtaaa tgtagcctct gcgcttaata ttgctgcatt taacgttggg attgccattg
    15901 gttcttttgt aggcggccta atcgttgatt ccattggtct tgttcatact ccctggattg
    15961 gaggagttat ggttttagga gcggtcttat taacggcatg gagccgttca atagaagcaa
    16021 aagcaaaata aaaaaagaga tcatcgcttt ttgcgatgat ctcttagtta taaaaggaga
    16081 ggaagcaaat gactacattt caagatcaca aggcatatcg tcgtatgtac aaggaaaatg
    16141 agttaacatt aggtttgcat atgccgcttg aaaattatca gtatgcgacg cctacgatgg
    16201 agaaacaagt agagctcacg cagcttgcag aacaatatgg gtttacaagc ttatggttcc
    16261 gtgatgtcct tcttcaagat ccatcatttg gtgatccagc cacgggtcaa atttatgata
    16321 tgcttattta tttgacttat cttgctagtc aaacaaaaga aattgctctc agtacatctg
    16381 ctattgtgct gcctttacgc catccgcttc gtgtagctaa agaaatggca accattgatc
    16441 agctttttcc tgagcgtttg attatgggcg tatcttctgg agaccgtcaa gcagattttc
    16501 gagggttagg tattcctcat gaagaaagag gaaagcgatt tgtagaggct tttcattatt
    16561 tagaagaagc gttaaataaa gaatttccat ctatccagtc tgctttggga tccattcaag
    16621 gagcaaacgt tgttcctaag ccaacgaaac gtattccaac gatgattacg ggatacagtc
    16681 agcaaaatat ggagtggttt gcacagtatg gagacggctg gatgtattat cctcgtcatc
    16741 cgcattttca ggcagaagct attacacaat ggaggaaact ggttgacagc tatcatccag
    16801 gcgtttttaa accttttaca cagcctatgc atctagattt ggcagaagat ccaaatgaaa
    16861 tgccaacgcc aattcgttta ggatttcgag caggtcgtaa tacattaatt gagcttcttt
    16921 ctatttataa agaaatagga gttaatcacc tcttttttgc tctttttgat agtaagcgtc
    16981 cggctgaaga agtcatacat gagcttggtg aagaagtgct tccgcatttt ccaggtcata
    17041 tgtagcaaaa agaggctggg acaaaagcag tttagttaaa ggaaaatccg aacgagttag
    17101 attcttaatg gagaatctaa ctcgttcgga ttttttattg gtaggatgaa cgtagatttc
    17161 attgatttcg ttatgtctca atctcttttt atatatttca agttatagga tatataagaa
    17221 atatgtatta gagttattga tcctatgttc tttataataa aagtatccat tatctccttg
    17281 gatgatacaa agtcgaaagg agaaacacga tgagtgttgc atttttattt ccaggacaag
    17341 gcgcacaata tgctgatatg cttcatgata taaaaaataa atggggatta gctagtacgt
    17401 ttgaagaagc aagtgag
//
