LOCUS       NZ_CP064275            20819 bp    DNA     circular CON 17-APR-2024
DEFINITION  Priestia megaterium strain B1 chromosome, complete genome.
ACCESSION   NZ_CP064275
VERSION     NZ_CP064275.1
KEYWORDS    RefSeq.
SOURCE      Priestia megaterium (Bacillus megaterium)
  ORGANISM  Priestia megaterium
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 5078588)
  AUTHORS   Wang,C., Jia,Z., Zhao,J., Wang,X., Liu,Z. and Ji,F.
  TITLE     Comparative Genome Analysis of key enzyme genes in ammonia nitrogen
            Metabolism Pathway of bacteria from different sources
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5078588)
  AUTHORS   Wang,C., Jia,Z., Zhao,J., Wang,X., Liu,Z. and Ji,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2020) Guangdong Key Laboratory of Animal
            Conservation and Resource Utilization, Guangdong Public Laboratory
            of Wild Animal Conservation and Utilization, Institute of Zoology,
            Guangdong Academy of Science, Microbial Pathogenic Ecology Group,
            105 Xingang West Road, Guangzhou 510260, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. V1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 252.67x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022811885.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 10:12:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,571
            CDSs (total)                      :: 5,383
            Genes (coding)                    :: 5,338
            CDSs (with protein)               :: 5,338
            Genes (RNA)                       :: 188
            rRNAs                             :: 15, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 137
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 12 of 46
            Pseudo Genes (incomplete)         :: 26 of 46
            Pseudo Genes (internal stop)      :: 11 of 46
            Pseudo Genes (multiple problems)  :: 4 of 46
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:16:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 397996
            Orig. end                         :: 418815
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP064275.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20819
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="1"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..20819
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20819
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10819](+)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10819
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    20..33
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 22.43"
                     /tool="antismash"
     misc_feature    436..450
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.95"
                     /tool="antismash"
     misc_feature    complement(952..970)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 27.39"
                     /tool="antismash"
     misc_feature    1029..1041
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 20.68"
                     /tool="antismash"
     gene            1101..1574
                     /locus_tag="IQ966_RS02365"
     CDS             1101..1574
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013058548.1"
                     /locus_tag="IQ966_RS02365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YtxH domain-containing protein"
                     /protein_id="WP_050689925.1"
                     /transl_table=11
                     /translation="MTKENVKETVGKETKGGSKELLVGTLVGSVVGVTTALLVAPKSGK
                     ELRASIQDGANQALAKTGQVKETVYAKGQDLKQRTARLSQKVSEQSSQVVNKVKSLRTA
                     KEEPLAETAAAEELETTEIVQDPVLEAEAVQEELNTITQEVSDLEQSVDETRR"
     gene            complement(1618..1956)
                     /locus_tag="IQ966_RS02370"
     CDS             complement(1618..1956)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016765165.1"
                     /locus_tag="IQ966_RS02370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_013058549.1"
                     /transl_table=11
                     /translation="MVGICSWKCAVSGISIASVYSKQPSWQRECYLVTPGKVYYESCYQ
                     GYGEFAGMDIFCLMRESGAEKEDSEGVAAFKPKIVLAKYYSGQRYEELPESEPCPYGGY
                     FFEGWKEG"
     gene            complement(2247..2507)
                     /locus_tag="IQ966_RS02375"
     CDS             complement(2247..2507)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013058550.1"
                     /locus_tag="IQ966_RS02375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_016765166.1"
                     /transl_table=11
                     /translation="MTTTTNLTLGYKMYTIDFSRPTQSNEALLDFFKDVQHRQGNNAES
                     FSKTLMEIEEIKTKYVDHEIVLLAKSLEQQISRSNAEKLEV"
     misc_feature    2590..2603
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.52"
                     /tool="antismash"
     misc_feature    2591..2604
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.7"
                     /tool="antismash"
     gene            complement(2644..3252)
                     /locus_tag="IQ966_RS02380"
     CDS             complement(2644..3252)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014458705.1"
                     /locus_tag="IQ966_RS02380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_014458705.1"
                     /transl_table=11
                     /translation="MDVTFLTNVIRHILERDNFVIDFEESDEQNIHFWHRDEPFRLFIS
                     EQNENYLVWTVFENEKEQWSGKTFLQEVTNTCKDCSEPIAHWIYKDGGKEGYCDRCLQT
                     FGNAYFTNADDIKQNLHLIDGSICFITDETGSDWWIAYEGFKRVIARLEENPDQKVEMY
                     KPEYPVFKWEIHFLEENNSASFKFKDDFASGIVPKGRIL"
     gene            3426..3791
                     /locus_tag="IQ966_RS02385"
     CDS             3426..3791
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013084538.1"
                     /locus_tag="IQ966_RS02385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF3221 domain-containing protein"
                     /protein_id="WP_014458704.1"
                     /transl_table=11
                     /translation="MKFTVQSTALFLLFFICLSGCSDNAEQSKKPAGYLVQGRILEVKQ
                     NGILIANVIPRDTALDYTTKEILGSSDVQPIYVKIPDNQTYKKGQLVKAWLKKDKSVQY
                     SVPAKGEAVEVNILKQP"
     gene            complement(3803..4735)
                     /locus_tag="IQ966_RS02390"
     CDS             complement(3803..4735)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013058553.1"
                     /locus_tag="IQ966_RS02390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="patatin-like phospholipase family protein"
                     /protein_id="WP_014458703.1"
                     /transl_table=11
                     /translation="MKADAVFEGGGVRGIAFTGAIEAMEESNVEWQRLAGTSAGALIAA
                     LLASGYKSGEIRRELQNMDYAKFRGKTIINRIPLIGNLIELMVHLGFYKNDYLEKWVYG
                     LLREKGIETFADLPADKLKIIASDISNGQMLVLPDDLPRYGMQPSDMKISQAVMMSASI
                     PFFFRPVIWKSKGFKRSYIVDGALLSNFPIWLFDTEHPRFPTFGFRFVKEEIDTAAIIP
                     TPVHLFKSMFTTMLQAHDLRYLNAETKERTVQIPTGVITATDFALKEEEIEFLYESGYI
                     SAKRFLEKWDFEQHKMNRTERPLTRRKLP"
     misc_feature    complement(4705..4714)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 16.22"
                     /tool="antismash"
     gene            complement(4863..6773)
                     /locus_tag="IQ966_RS02395"
     CDS             complement(4863..6773)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1243: ABC
                     transporter, permease component"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013058554.1"
                     /locus_tag="IQ966_RS02395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter permease"
                     /protein_id="WP_243549835.1"
                     /transl_table=11
                     /translation="MTVFDLVKKNMRKNISRYSLYFFSMIFSIIVYYVFATLQYDQSIS
                     KVIGEEMRLQGIFNASNYVLLTFIVVFIWYTNSFFIRQRKRELGLYHLVGIEKRTIGKM
                     IFYENMLLGIFSLVVGILLGTVFSRLFVLFLLKLMGLSLSITLTFSLQAVLKTAVVFLI
                     VTIFISFQGYFIMYRYTLLDLFQAESKSERVNKMSSKRSIVVGGLGILLVGYGYYLSSG
                     NIVTPKFFIIVAVVLSSVIFGTFLLFKATIEWLMGLYREHASGYYSFANMLSVSTMMYR
                     IKANARVLTLITILSATTLVAVGVTYSSYYNTKADARSMQPYDYTLHSKNAAQDFIDVL
                     RELDIQSNQYVYHIVKHSADLSDFRFITSGYYGDGERISFLDEEEVRKNGVSVPELSAN
                     ETVIFDSMAKGKVFKPSTGKTAAVPLKESEQLALKVIKSEPSLILDSESAGYQFVVNHD
                     TFIKLSKSGQKQAVYVFNTGDGNKQSELTKNYHQIAKKAGESRTSYYDVFQTGLMSKGL
                     FIFIGGFLGLVFLMATGSIIYFKQISEAEQERKRFEVLRKLGFSVSQMMSAVRKQQLFT
                     FGLPLVIGILHSLFALKVIMNLTGESLIVPVLIADTVYGVIYFIFYWLTMNYYRMVIKR
                     L"
     gene            complement(6760..7527)
                     /locus_tag="IQ966_RS02400"
     CDS             complement(6760..7527)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013058555.1"
                     /locus_tag="IQ966_RS02400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_115654716.1"
                     /transl_table=11
                     /translation="MSIVLEGKNVTKTFGSRGNVYEALQGIDFTIEEGEFVGIMGPSGA
                     GKTTLMNILSTIDRPTDGEVWIQGENVALMKDKKLSAFRRNHLGFIFQDYNLLDTLTVK
                     ENILLPLTVTKMSKQEMEAKLKATATRLGIYELADKYPYEISGGQKQRVAAARAMITNP
                     AVIFADEPTGALDSKAASSLLEKMSQLNQEDRATIIMVTHDPLASSYCSRVLFIKDGQI
                     YTELFKGEQTRQAFFKRIMNIQGALGGEGYDRI"
     gene            complement(7524..7880)
                     /locus_tag="IQ966_RS02405"
     CDS             complement(7524..7880)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013084542.1"
                     /locus_tag="IQ966_RS02405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF1093 domain-containing protein"
                     /protein_id="WP_014458700.1"
                     /transl_table=11
                     /translation="MKRKWISLLALILIVGACIGGVKIYQSAATEHQYGMIPKEAKKVD
                     DYGRVQYTISVINEKGQTTFQTFTTIKQLKEGTVIDLFVRGNEVRKYDILTKDELPQRV
                     KSVWPIKERKEEEK"
     misc_feature    complement(7908..7924)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 24.57"
                     /tool="antismash"
     gene            complement(7968..9002)
                     /locus_tag="IQ966_RS02410"
     CDS             complement(7968..9002)
                     /EC_number="2.7.13.3"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016765172.1"
                     /locus_tag="IQ966_RS02410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sensor histidine kinase"
                     /protein_id="WP_243549837.1"
                     /transl_table=11
                     /translation="MWKSFLREKLSWILFVLLWLSVIDALLLLEYDIEVSSTSFWYLNL
                     LTLCMLILFLLWRGVKERRFIKRLSLMLQNDAYDLQEMHSYVRTKADEDMLSLLQRIDR
                     VYRRQTHALHVQQREQQEYITTWVHEIKTPLTAMKLLINARRTELYKWPWLKDIEDEWM
                     KIDGLVDQNLYAARLRHLEKDYQVHAVSLSELVFPNVAYFSKWCIEKRLHIDEKNLDTV
                     VHTDPKWMQFVVRQVLSNAIKYSPLEGKLSFFSEETPKYVKLFIVDQGEGIPPQDLPRV
                     FEKGFTGNVGRKSKGATGLGLYFSKKITDALGHRIEVHSLLHEGTTVILTFYKPNSFDN
                     LSKM"
     gene            complement(9004..9675)
                     /locus_tag="IQ966_RS02415"
     CDS             complement(9004..9675)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014458698.1"
                     /locus_tag="IQ966_RS02415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_208732973.1"
                     /transl_table=11
                     /translation="MKILIVEDDSKIYELVKEKFEQWSFEVVGVEDFQKVMEVFIAESP
                     ELVILDVNLPVYDGFYWCQQIRTVSKTPIVFLSSREHPMDIVMAMNVGADDYIQKPFNL
                     DVLIAKVQALLRRTYSYGDTISDVIEWNGAVLDLKKGSLHYNDREVHLTKNEFFILRLL
                     LEERGKIVSREELMRRLWEDEKFVSDNTLSVNITRIRTKLEEIGLNDQIVTKKGQGYLV
                     N"
     misc_feature    complement(9765..9779)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 24.37"
                     /tool="antismash"
     gene            10001..10819
                     /locus_tag="IQ966_RS02420"
     CDS             10001..10819
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_phytoene_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014458697.1"
                     /locus_tag="IQ966_RS02420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="WP_014458697.1"
                     /sec_met_domain="PT_phytoene_like (E-value: 4.1e-26,
                     bitscore: 90.1, seeds: 61, tool: rule-based-clusters)"
                     /sec_met_domain="phytoene_synt (E-value: 4.7e-08, bitscore:
                     30.8, seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVPNKLRDNAMVMLKETSRTFFIPISHLPAELQDAVGSAYLCMR
                     AIDEIEDHPELEAGVKSRLLYAISDLLKKSFNEDEYMALVGPYQADLPDVTLQLGNWIA
                     LCPTGVADQVLDATSIMAKGMADWVEKDWHIQNEADLDDYTFYVAGLVGVMLNDIWKWY
                     DGTETDKELAIAFGRGLQSVNILRNTSEDSERGVSFFPNNWSREDMFAYARRNLSLGKK
                     YLEDVKSLPILHFCKIPLALADGTLNALMKGKEKMTRDDVNKTVSEVVDM"
     gene            complement(10855..12804)
                     /locus_tag="IQ966_RS02425"
     CDS             complement(10855..12804)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016765174.1"
                     /locus_tag="IQ966_RS02425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FUSC family protein"
                     /protein_id="WP_208732972.1"
                     /transl_table=11
                     /translation="MNDDKKSPTYNHTEIHHKIEASPKAPGIKAAFRFNHASSPWVRGI
                     SSGLSSSLPILIGILFGHLQYGLIASIGGFTYLYTKDEPYAQRAVKLFLALLGITLCFT
                     LGSLFAEVEWIKTILFGVIGAVSVYIFGALKIPGPSAMFFVLSFAVSTSIPAPNLQAVF
                     LHAGLVFLGGALSWCISMSGWLINPRGPETAAVAKAYRQIASFVGSLGTESFHAEQHKT
                     VVELRAAETAMKQAKINRKKSSFSHRLFLLNQKAEGLFIFVMAAANDFNPAVSKKAQAV
                     IETFASEMATLKKHKKIRDVREEIEELSIKEELRTLLLDVNDVLQMKELKETEYIQFSQ
                     GSVKARLKAAFHKRSYVLKNALRYGIIMIIAAAVAYTTDIPRSYWIPLTCAGVMLGATV
                     RATVQRGIQRSVGTILGILIGTGILMLEPTGIVIALIVFVLQSTIEIVIVKNYALGTLF
                     ITPNALLIAESGHPDKAISYFTSARVIDIVIGSVIGVVGTVLVFKRASSLRLPYLLGDV
                     IRKEGQFLEMLSTEQDEERIKQERNKLQGELVNLRALYDNVIGEYSKTAVKLEGLWPAV
                     VATQQLGYMLLASYEGYELSETEKRELQETFAQLAAAAEQKRAPKLSSVPTLKRYPIIQ
                     GEITALYESLQVEI"
     gene            complement(12961..13221)
                     /locus_tag="IQ966_RS02430"
     CDS             complement(12961..13221)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016764952.1"
                     /locus_tag="IQ966_RS02430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="WP_013058561.1"
                     /transl_table=11
                     /translation="MEYDKQVKNRLKRIEGQLKGVLNMMEQGKDCRDIVTQLSAARSAI
                     DRTMGVIVSTNLEQCVRESIEKGENTENLVKEAVELLVKSR"
     gene            13623..14039
                     /locus_tag="IQ966_RS02435"
     CDS             13623..14039
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390452.1"
                     /locus_tag="IQ966_RS02435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NucA/NucB deoxyribonuclease domain-containing
                     protein"
                     /protein_id="WP_181170505.1"
                     /transl_table=11
                     /translation="MASLFIIIACVLGFIGHAAFLAPEKTEAEANYDATLYFPLDRYPE
                     TGDHIRDAIAAGHSSVCTIDRDGAEANREESLKGYPTKTGYDRDEWPMAMCEEGGAGAD
                     IRYISPSDNRGAGSWVGNQLEQYPDGTRVQFIVQ"
     gene            14075..14482
                     /gene="comJ"
                     /locus_tag="IQ966_RS02440"
     CDS             14075..14482
                     /codon_start=1
                     /gene="comJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016765176.1"
                     /locus_tag="IQ966_RS02440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="competence protein ComJ"
                     /protein_id="WP_116739299.1"
                     /transl_table=11
                     /translation="MKTKWKEQELTMSYHQFTLFQKGQPKPHHDWSDEDIQKGYVADDK
                     AISFEAVSNTKAIIEVWVNETPDASKAEKTTRLPFHVESDGIEIKSVLSETLSYDIPKG
                     YYYVTCLTIPLQEKTNSGLYLVKYLLHFEGQ"
     gene            complement(14714..15304)
                     /locus_tag="IQ966_RS02445"
     CDS             complement(14714..15304)
                     /EC_number="3.4.-.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014458692.1"
                     /locus_tag="IQ966_RS02445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_140451079.1"
                     /sec_met_domain="Abi (E-value: 6.7e-14, bitscore: 50.3,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFQIGSNYFIEWDLSKKMKIIVNRVLLLFIIGGLLLSVLLSKQRV
                     FLFASLPDWQKRVVMPQHSIQLTYFLMFGLIGSATIFVPLLFAEDISYTKSFVIYGIVF
                     SIINAILEEVLWRGIMLSHLKSSLSTFYAVFITSIGFGLLHISIGIPVVISLLFSFGGF
                     FYAFVVLKTKSIYPAVVFHFIINLGMVFNGWIF"
     gene            complement(15693..18011)
                     /gene="helD"
                     /locus_tag="IQ966_RS02450"
     CDS             complement(15693..18011)
                     /codon_start=1
                     /gene="helD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014458691.1"
                     /locus_tag="IQ966_RS02450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="WP_208732970.1"
                     /transl_table=11
                     /translation="MKDKNLTEEQQRVDWVVEEISQKAEKIGKSVGNVKGEIVDLRSTF
                     WDDVTVNMDEPDDVIETFTSIKQQAELLAEREITHRQAYDQLKTLKKLTQSPYFGRFDF
                     IENGEKEAEAIYVGVGSLMDQKDEEFLIYDWRAPISSLYYDYSPGKAQYETPGEAIKGE
                     MKLKRQFIIENSVIKSMFDTGITIRDELLQEVLGHNASSQMKSIVATIQKEQNQIIRNE
                     KNKVLIVQGVAGSGKTSAALQRVAYLLYHHRKQLTAENMMLFSPNPLFNSYVSTVLPEL
                     GEDNMKQTTFQQYADGRLGNEFEVEDLFVQIEYLLTEKDAATKKQKMQEIRYKASREFK
                     KQVDAYIEELSTKDIIFKNIKFRGEIVLSARRLLSYFYSLDTGISIPNRMQLVAEWALK
                     LINQLEKLERKKDWVQEEIQLLEKEDYVEAYQHLQKKEGYTESSFDDFDREQAYLAKAV
                     VAKKMKFIKQAIKQLKFIDIRRIYMQLFERNHLFSHVEGWEDTAKNTVQRVMNMKLSYE
                     DVTPYLYIQDQIEGRKANPVIRYLFIDEAQDYSPFQLAFLKELFPHARMTLLGDLNQAI
                     YAHALNAPTILSSELYEEKEAETLTLTRSYRSTRQIVEFSRDMVANGHLIDPFNRNGAK
                     PTVTAAGDVKELHEAIINKINELKQRNYKTIAIIAKTAEESRQAFQALPDSMSARLLTK
                     ETSSFEKGMLVLPAYLAKGIEFDAVIIYNASSECYQDEYERNLFYTACTRAMHELHLFS
                     LGEISPFISNKETYEYHGK"
     gene            complement(18255..18380)
                     /locus_tag="IQ966_RS27775"
     CDS             complement(18255..18380)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IQ966_RS27775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_014458690.1"
                     /transl_table=11
                     /translation="MPFIFKEAVVGLFTAAAAGLLAFQGVEVVTAIIHMLTFWKQ"
     gene            complement(18521..19036)
                     /locus_tag="IQ966_RS02455"
     CDS             complement(18521..19036)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: ECH (6-132). E-value: 5.8e-18. Score:
                     57.0. Matches aSDomain: nrpspksdomains_IQ966_RS02455_ECH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023: enoyl-CoA hydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016765179.1"
                     /locus_tag="IQ966_RS02455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="WP_115654720.1"
                     /transl_table=11
                     /translation="MKRFIKVEKRNHVAVIRMNQSMLNRLNSEVMYELQETMNELQHDS
                     SVGSIVFMGEYYKMNGDHRTTSFLREETGRLYQPIHAVAARAIFQRISCSAKQTIALLT
                     NFTFGGGYELALACDVRIAEEPVQFKLTNGMTVSAQEACNMGKVQHVVQKGKGLEYSLA
                     LNALEKEA"
     aSDomain        complement(18641..19018)
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IQ966_RS02455_ECH.1"
                     /evalue="5.80E-18"
                     /label="IQ966_RS02455_ECH.1"
                     /locus_tag="IQ966_RS02455"
                     /protein_end="132"
                     /protein_start="6"
                     /score="57.0"
                     /tool="antismash"
                     /translation="VEKRNHVAVIRMNQSMLNRLNSEVMYELQETMNELQHDSSVGSIV
                     FMGEYYKMNGDHRTTSFLREETGRLYQPIHAVAARAIFQRISCSAKQTIALLTNFTFGG
                     GYELALACDVRIAEEPVQFKLT"
     gene            19279..19647
                     /locus_tag="IQ966_RS02460"
     CDS             19279..19647
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013084552.1"
                     /locus_tag="IQ966_RS02460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_014458688.1"
                     /transl_table=11
                     /translation="MKYDLTKWEPHGYSCPVEAAVDVVGGKWKSVILYQLSKERRRFNE
                     LRRLIPGVTQRMLTLQLRELERDGIIHREVYKQVPPKVEYYLTPFGETLIPIIELMFQW
                     GYNHTDKIAEARIQAEKE"
ORIGIN
        1 cccaccgcgt aaatcacatt ataaatatta tgaaaatcgt ggccatccgc tttgattcct
       61 tgcggtgaaa agacaaacac gtcttttccg tccagtttaa agtaatccgg gcactcccac
      121 atatagccaa agtcgggaag cgatgtttta acttcgcctt taaatgacca ctgaagcgca
      181 tcgcttgatt catacacaat taatgcgccc gtttcgtttt cacgctgagc gcctagcagc
      241 atataatatg tatcattttc tttccatact ttcggatcgc gcacatgccc tgtataccca
      301 tcaggaacgc ctcgtacagc tggattatgc gtgtattttt ctacacttcc atccctgttt
      361 agcatcgcga tacactgatt agcagaacgt gagccgtctt cgtattttac attccccgta
      421 taaaacagca gcccttgacc atctttttca atcgcgccgc ctgagtatgc gccgtgcgat
      481 tcatattctt ccgttggcgt tagggcaacc ggaaggcgtt cccacgttac taaatccgta
      541 gatttcacat gagcccagtg cttcattcca tgcatcgcac cgtacggata ccactgataa
      601 aacacgtgat attcaccgtt taattgaatc aatccgtttg gatcattcat cagcccgtac
      661 tgtggatgaa tatgataaat cggctgccag ctgtctttga ttgctttgtc ttttaatgct
      721 tctaactctc cagattttgc ttctaaaatc gttctgtact tcgattcagc catttcttca
      781 atccctccag catttaaaaa aagagaacgt attccccttg ccctctatga tataaaattt
      841 caagtgaaaa tgctaccaaa gaacgaatgt tgtcgttttc ttgtgtcgga ttttatagat
      901 aagttttttt ctgtttttat agcagaaaag aaggtttttc tgtcaggtgg ttgaatacta
      961 tatacaatca aatttcacca gcctgtacga gcgtccgacg gcacttgcag atttttttag
     1021 cgaatcattt gcgttttgca ggaagtattc aacaaaatgg taaacccttc atattacata
     1081 ttataaaagg aggaaatgtc atgacaaaag aaaacgtaaa agaaacagtg ggtaaagaaa
     1141 caaaaggtgg aagcaaagag ttattagtcg gcacgttagt aggtagcgta gtgggtgtaa
     1201 caacagcttt actagtagcg ccaaagtcag gaaaagagct gcgtgcttct attcaagacg
     1261 gagcgaatca agcacttgct aaaacaggtc aagtgaaaga aacggtatat gcaaaaggcc
     1321 aagatctcaa acagcgcacg gctcgtcttt cacaaaaagt ctccgaacag tcttcgcaag
     1381 ttgtcaacaa agtgaaatct ctgcgcactg ctaaagagga gccgcttgca gaaacggctg
     1441 cagcagaaga gcttgaaacg acagaaatcg ttcaagatcc tgttcttgaa gctgaagcgg
     1501 tgcaagaaga attaaataca attacgcaag aagtaagtga cttagaacaa tctgtcgatg
     1561 aaacaagacg ttaataacaa gctgtagaga tatctctaca gcttttatgt atattcgcta
     1621 cccttccttc cacccttcaa aaaaatagcc tccgtacgga cacggctctg attccggcag
     1681 ttcttcataa cgctgtccgc tgtaatactt cgccagcaca atcttaggct taaaagccgc
     1741 taccccttca ctgtcttctt tctccgctcc gctttctctc atcaaacaaa aaatatccat
     1801 tcccgcaaac tctccgtacc cttggtagca cgactcataa taaactttac caggtgtcac
     1861 caaataacac tcccgctgcc acgacggctg cttactgtag acactcgcaa tacttattcc
     1921 actcactgca cacttccagc tgcaaattcc aaccatataa cgcctccaac gacttgatag
     1981 tctattcatc cacaactttt gataaaatca aacgtcctga gacacgtaca gaactttatc
     2041 ctcgaccaaa aacctatact accagattcc ctaccctctt gaattctccc caaaaggttc
     2101 acctatgcgg aagttggcag cccgcaaggg aaggcaactt ccgcataggt ctagcactgt
     2161 atccttcgct gcactttagc caaggataca gtgctacatc gataaaaata aaaaaggtct
     2221 gataaatcga ccatttctgg tgattatcag acctcaagtt tttctgcatt tgagcggctt
     2281 atttgctgtt caagcgattt agccagcagt acaatttcat gatctacata ttttgttttg
     2341 atttcttcga tttccattaa tgttttagaa aagctttctg cgttatttcc ctgtctgtgc
     2401 tgcacatctt taaagaaatc aagcagtgcc tcgttagact gcgtcggacg gctaaagtca
     2461 atcgtgtaca ttttgtaccc cagtgttaaa tttgttgtag ttgtcataat tatttccccc
     2521 attcttccta tagtgatata tcttctactc tttacccttt aattccttct ttttaaccta
     2581 aattaattaa ttttttcaaa aaaacttccc attataagaa aaaacaccct tataacaagg
     2641 atgttagagt attcttccct tcggtacaat tccagatgca aagtcatctt tgaacttaaa
     2701 gctagcagaa ttgttttcct ctagaaaatg tatttcccat ttaaagacgg gatattcagg
     2761 cttgtacatt tcaactttct gatcggggtt ttcttctagt cttgcaatta ctcgtttgaa
     2821 tccttcatat gcaatccacc agtcgctccc ggtttcatcg gtaataaaac aaatggaacc
     2881 gtcaattaaa tgaaggtttt gcttgatatc atcagcattg gtaaaatacg catttccaaa
     2941 cgtttgaaga caacgatcgc aatacccttc tttgccaccg tctttataaa tccagtgcgc
     3001 gattggctca ctgcagtctt tacacgtatt cgtcacttct tgaagaaatg tcttgccgct
     3061 ccactgttct ttttcatttt caaacacggt ccatactaaa tagttctcat tttgttctga
     3121 aataaaaagg cgaaacggct cgtctcgatg ccaaaaatga atattttgtt cgtctgattc
     3181 ttcaaaatct atgacaaaat tatctcgttc taatatatgt cgaatcacat tggttaaaaa
     3241 ggtaacatcc atacaaggac ctcccgtact aagcatagct tctagtaata taacgtttat
     3301 gagttaaaac atatcacgcg cgctgaaata acgtcaatga ttacctctta ctttctccga
     3361 aaactggaaa agaaatggtc aaacgcgcta taatggtagt catgttgaaa cggaaaggag
     3421 attttatgaa gtttaccgtt caatcaactg cactttttct tctctttttt atttgtttaa
     3481 gcggctgttc ggataacgcc gagcagtcaa aaaagccggc cggctatttg gttcaagggc
     3541 gcattttaga agtgaaacag aatggaattc ttattgcaaa cgtcattccg cgagataccg
     3601 cgcttgacta tacaacaaaa gaaatacttg gttctagcga tgtacagcct atctatgtaa
     3661 aaattccgga taatcaaaca tacaaaaaag gacagctcgt taaagcatgg ctcaaaaaag
     3721 acaaaagcgt tcaatattcc gttccggcaa aaggcgaggc tgtggaagtc aacattttaa
     3781 aacagccgta gaaaagctcc tgttacggga gctttcttcg cgtgagcgga cgttcggttc
     3841 gattcatttt atgctgttca aaatcccact tttctaaaaa gcgctttgct gatatataac
     3901 cagattcata taaaaactcg atttcttctt cctttaaggc aaaatccgtt gccgtaatca
     3961 cacccgttgg aatttgcacc gttcgttctt ttgtctccgc gttcaaatac cgcaagtcgt
     4021 gagcttgcag catcgttgta aacatacttt taaataaatg gaccggcgtc ggaataatag
     4081 cagctgtatc aatttcttct tttacaaatc gaaaaccaaa cgtcgggaaa cgcggatgct
     4141 ccgtatcaaa cagccaaatc ggaaaattgc ttaaaagcgc tccatcgaca atgtatgacc
     4201 tcttgaaacc tttggatttc caaatgacag gacgaaagaa aaaaggaatg gacgcgctca
     4261 tcatcacagc ttgtgaaatc ttcatatcgc tcggctgcat tccgtagcga ggcaagtcgt
     4321 caggcagcac aagcatctgt ccgttggaaa tgtctgaggc aataattttc agcttatctg
     4381 ccggcaaatc cgcaaacgtt tcgattcctt tttcacgcag cagaccgtac acccactttt
     4441 ccaaataatc atttttataa aaacctaaat gaaccatcag ttctattaag ttcccaatca
     4501 ggggaattcg gttaatgatc gtttttccgc gaaacttagc atagtccatg ttttgaagct
     4561 ccctgcgaat ttcgccactt ttatagccgc ttgccaaaag agccgcaatc agtgctccgg
     4621 ccgacgtacc tgctagacgc tgccattcta cattgctttc ttccattgct tcaatcgcac
     4681 ccgtgaatgc aattcctcga acgcctcccc cttcaaacac agcatctgct ttcacatttt
     4741 tcccctccta tacaactctc tagtttttat ataccctttt ttatattcag attttcgcta
     4801 ctccatacct tttattttcc aaacaaaaaa agactgtttc atttatcgaa acagtctttt
     4861 ttttacagtc gctttattac cattcggtag taattcattg tcagccagta gaaaatgaaa
     4921 taaataaccc cgtatactgt atccgctatt aaaacaggga ctattagact ttcaccagtt
     4981 agattcataa taaccttcag cgcaaataga ctatgcaata tgccaattac taaaggcaaa
     5041 ccaaacgtaa acagctgctg ttttcgaacg gcgctcatca tttgagacac tgaaaaccca
     5101 agcttacgca gtacttcaaa tcttttgcgc tcttgttcgg cttctgagat ttgtttaaag
     5161 taaataatac tgcccgttgc catcaaaaag acgagcccta aaaatcctcc aataaaaata
     5221 aacaatcctt tactcattaa gcctgtctga aacacatcat agtatgaagt acggctttcc
     5281 ccagcttttt ttgcgatctg atgatagttc ttagtaagtt cactttgttt gtttccatca
     5341 cctgtattaa atacgtacac cgcttgcttt tgtccagact ttgaaagttt gataaacgtg
     5401 tcgtgattga ctacaaactg atagccggca ctctcagaat caagaataag cgaaggttct
     5461 gatttaatga cttttaaagc aagctgttct gattccttca gcggcacagc tgcggtttta
     5521 cctgtactag gtttaaacac tttccctttt gccatcgaat caaaaataac ggtttcattt
     5581 gcgcttaact caggaaccga aacgccattt ttccgcactt cttcttcatc taaaaagctg
     5641 attcgctcac catctccgta ataaccggat gtaataaatc gaaagtctga taaatcagcg
     5701 gagtgcttga cgatatgata cacatactga tttgattgga tatctaactc acgcagtaca
     5761 tctataaagt cttgcgctgc gtttttgctg tgcagcgtat agtcataagg ctgcatgctt
     5821 ctggcgtctg cttttgtgtt atagtacgag gaatacgtca caccaactgc tacaagtgta
     5881 gtagccgaca agatcgtaat taatgttaac actcgtgcat ttgctttaat tcgatacatc
     5941 atcgtagaaa cgcttagcat attggcaaac gaataatacc cgcttgcatg ttctcggtaa
     6001 agtcccatca gccactcaat tgtggcttta aaaagtaaaa atgtaccgaa aataaccgag
     6061 gataggacaa ctgctacaat tataaaaaac ttaggcgtca caatgttccc tgacgaaagg
     6121 tagtacccgt agccaacgag taagataccc agtcctccaa cgacaattga tcgctttgaa
     6181 ctcattttat ttacgcgttc acttttgctt tctgcttgaa acagatctaa tagcgtatag
     6241 cggtacataa taaaatagcc ttgaaacgat ataaaaatgg taacaattaa aaacactacg
     6301 gctgttttta aaaccgcttg aagagaaaat gtgagcgtaa tagaaagcga cagtcccata
     6361 agctttagta aaaacagcac aaacagcctt gaaaacaccg tacctaatag aataccgacc
     6421 actaaagaaa aaatcccaag cagcatattc tcatagaaaa tcattttccc aatcgtacgc
     6481 ttttcaatac cgactaaatg atacagccca agctctctct tacgctgtct gataaaaaat
     6541 gagtttgtgt accaaataaa gacaacaata aaggtaagca gaacataatt tgaagcatta
     6601 aaaataccct gaagcctcat ctcttctcca ataacttttg aaatggattg atcatactga
     6661 agcgtagcaa acacatagta cacaataata ctaaagatca ttgaaaagaa ataaaggctg
     6721 taccggctaa tatttttacg catattcttt ttaacaagat caaatacggt catagccttc
     6781 accgcctaat gcgccttgga tgttcataat tcgtttaaaa aatgcttgtc tcgtttgttc
     6841 tcctttaaac agctccgtat aaatttgtcc atctttaata aacagtacgc ggctgcagta
     6901 gcttgaagcc agcggatcat gcgtcaccat aataatcgtg gcacggtctt cttggtttaa
     6961 ttgcgacatc ttttcaagca ggcttgaagc agcttttgaa tcaagagctc cggtaggttc
     7021 atcggcaaaa ataacggctg ggttcgtaat cattgctctc gctgctgcaa cgcgctgttt
     7081 ttgtccgcca gaaatctcat acgggtactt atctgccagc tcatagatgc ctagtcgcgt
     7141 tgcggtagct ttcagcttcg cttccatctc ttgcttggac atttttgtaa ccgtaagcgg
     7201 cagaagaata ttttctttta ccgttaacgt atccagtaaa ttataatctt gaaaaataaa
     7261 tcctaaatga ttgcgcctga aagcagacag cttcttatct ttcatgagcg ctacgttttc
     7321 tccttgaatc catacctcgc catcggtggg acggtcaatc gttgaaagaa tgttcatcag
     7381 cgtcgttttt ccagctccag acggtcccat aataccgaca aactctcctt cttcgattgt
     7441 aaaatcaatt ccttgtagcg cttcgtatac gtttcctctt gatccaaacg ttttggttac
     7501 atttttccct tcaagaacta tactcacttt tcttcctcct ttcgttcttt tataggccat
     7561 acgcttttga cccgctgcgg cagctcatct tttgttaaaa tgtcatattt ccgaacttcg
     7621 ttacctcgaa caaaaaggtc aataaccgtt ccttctttta actgtttaat cgttgtaaag
     7681 gtttgaaacg ttgtttgtcc tttttcattt atcactgaaa tggtatattg aactcgtcca
     7741 tagtcatcca ctttttttgc ttcttttggt atcatcccgt attgatgctc cgttgcggcg
     7801 ctttgataaa tctttactcc tcctatacat gctcctacaa taagaatcaa agctaagagg
     7861 ctaatccact ttcgtttcat atgcgttccc tccttgtgta tagctctatc ttactttgta
     7921 acaaggaaag attcgtttga agcagatgac aatctcagct tgcaacctta catttttgaa
     7981 aggttatcaa aagaattcgg tttgtagaaa gttaaaatca ccgtagttcc ttcgtgtaaa
     8041 agtgaatgta cctctattcg atggccaaga gcatctgtga ttttttttga aaaatacagc
     8101 cctagccccg tagctccttt cgattttctt ccgacatttc ctgtaaatcc tttttcaaat
     8161 acgcgcggaa ggtcttgtgg tggaatgccc tccccttggt caacgataaa taacttcaca
     8221 tacttcggcg tttcttcact aaaaaaagat agctttcctt ctagagggct atatttaatt
     8281 gcattggata acacttgcct cacaacaaac tgcatccact ttgggtcagt gtgcactacc
     8341 gtatctagat tcttttcgtc aatatgcagt cttttctcaa tgcaccattt cgagaaatac
     8401 gcaacgtttg gaaaaaccag ttcgcttagc gagactgcat gtacctggta atctttttct
     8461 aagtgccgca ggcgtgcggc atataaattc tgatctacta acccgtcaat tttcatccat
     8521 tcatcttcaa tatccttcag ccaaggccat ttataaagct ctgtacgacg agcgttaata
     8581 agcagcttca tagcagtaag cggcgttttg atttcatgaa cccacgtcgt tatatattcc
     8641 tgctgttctc gctgctgtac gtgaagagcg tgagtttgtc tgcgatagac acgatctata
     8701 cgctgcagca gactcagcat gtcttcatct gctttcgttc gaacatatga atgcatttct
     8761 tgtaaatcgt acgcgtcatt ctgcagcatt aaacttaatc tttttataaa tcttctttct
     8821 tttactcctc tccataaaag aaacagaatt aacatacaca gcgttaaaag atttaagtac
     8881 caaaacgaag tagacgatac ctcaatatca tactcgagaa gcagcagagc atcgattaca
     8941 ctcagccata gaagaacgaa tagaatccag cttagctttt ctcttaaaaa acttttccac
     9001 atcttaattc accagatatc cttgaccttt tttggtcaca atttgatcat tcagcccgat
     9061 ttcttccagc ttggtacgaa tgcgtgtgat gttaactgaa agcgtattgt cacttacaaa
     9121 tttttcatct tcccataatc ttcgcataag ctcttctcgc gatacaatct tgcctcgctc
     9181 ttccaaaaga agccgtaaaa taaaaaattc atttttcgtt aaatgaactt ctcgatcgtt
     9241 ataatgcaaa cttccttttt ttaagtctag caccgcgccg ttccactcga taacgtctga
     9301 aatcgtgtct ccatacgaat acgttctgcg cagaagtgcc tgcactttag caataagaac
     9361 atccaagtta aacggctttt ggatatagtc atccgcccca acattcattg ccataacaat
     9421 gtccattgga tgttcacgag acgaaagaaa tacgatagga gtttttgaaa ctgtacgtat
     9481 ttgctgacac cagtaaaacc cgtcatatac aggtaaattc acgtctaaaa tgaccaattc
     9541 tgggctttct gcaataaata cttccattac cttttgaaaa tcttctactc caaccacttc
     9601 aaatgaccac tgttcgaatt tttctttcac taattcatat attttgctat cgtcttctac
     9661 aattaaaatt ttcatacctt cacctctgta ttctcctatg tattatgtat acccgactgt
     9721 tgagggtttt cttaaaagct catctaaccc gttagcatga cggcaatttt cttaaaaata
     9781 atctttttat gtctgtaaaa cgaagaatga acgagtatta tcgtagtgta aacttgtgca
     9841 tgatacaagt atctctgttt aaatactaca aaataatgac aatatcgtta ataagtaatc
     9901 tttaaactcc attaaatgct cattttttgt tactatagta tataagatga aaacttaaac
     9961 taatttgaca tgtggatatg taaaaaggag ctagttatca atgagtgtac ccaacaaatt
    10021 acgcgacaac gcaatggtga tgttaaaaga gacaagccga acatttttta ttccaattag
    10081 ccacttgccg gcagaattgc aagatgcagt aggatctgct tacttatgta tgagggcaat
    10141 tgatgaaatt gaagatcacc ctgagctaga agccggtgta aaaagccgct tgctttacgc
    10201 cattagtgac ctgttaaaaa agtcttttaa cgaagacgag tacatggcat tagtaggtcc
    10261 ttatcaagct gatttacctg acgtaacgct tcagctcggc aattggattg cgctttgtcc
    10321 gactggagtt gcagatcaag tattagatgc aacttctatt atggccaaag ggatggccga
    10381 ttgggtagaa aaagactggc atattcaaaa tgaagcggat ttagacgact atacgttcta
    10441 cgtagctggt ctagtaggcg tgatgcttaa cgacatttgg aaatggtatg acggcacgga
    10501 gacagacaaa gaattagcca ttgcatttgg acgcggcttg cagtctgtaa atattttgcg
    10561 caatacctcg gaagattcag agcgtggagt cagcttcttc cctaacaact ggagcagaga
    10621 agatatgttt gcctatgctc gccgcaatct gtcactagga aagaaatatt tagaagacgt
    10681 caaatcgctt ccgattcttc atttctgcaa aattccgtta gcgcttgccg atggaacgtt
    10741 aaacgcctta atgaaaggaa aagaaaaaat gacgcgtgac gatgtaaata aaacagtaag
    10801 tgaagttgtg gatatgtaaa agcaaaagca cggagcccat tggttccgtg ctttttatat
    10861 ttctacctgc agactttcat ataaagcagt tatttcccct tgaataatgg ggtagcgttt
    10921 cagggtcgga acacttgaaa gctttggcgc tcgcttttgc tctgctgctg cggctagctg
    10981 agcaaatgtt tcctgaagct ctcgcttttc tgtttctgac agctcatatc cttcatacga
    11041 cgcaagaagc atataaccta actgctgcgt agcgacaact gccggccaca gtccttcaag
    11101 cttgacggcc gttttagaat attctccaat cacattatca taaagcgccc gaagattaac
    11161 aagttctcct tgaagcttat ttctttcttg tttaattctt tcttcatctt gttcggtgct
    11221 tagcatttct aaaaattgtc cttcttttcg aatgacatct ccaagcaaat acggcagacg
    11281 aagcgaagaa gctcgtttaa aaactaatac cgtaccgacc actccaataa cagaaccaat
    11341 aacaatatca atcactcgag cggacgtaaa gtagctaatc gctttgtccg gatgaccgct
    11401 ttcagcaatt aaaagagcat tcggtgtgat aaaaagcgta ccgagcgcgt agtttttaac
    11461 aatcacaatt tcaatcgttg actgaagcac aaagacaatg agcgcaatga cgattcccgt
    11521 tggttcaagc atgagaatcc cggttccaat taaaatacct aaaatggttc cgacagagcg
    11581 ttgaatacct cgctgaaccg ttgcacgaac ggttgcccca agcatcactc ctgcacacgt
    11641 gagaggaatc cagtaagacc ttggaatgtc tgtcgtgtag gcaacagctg cggcgataat
    11701 cataataatt ccgtagcgca aagcgttttt aagtacatac gaacgtttgt gaaaagcagc
    11761 ttttaaccgt gcttttacgc tgccttgtga aaattgaata tactccgttt ctttcaattc
    11821 cttcatttga agaacgtcgt ttacatctag taataatgtt cgcagctctt ctttgatgga
    11881 cagctcttca atttcttctc gcacgtctcg aattttttta tgttttttta acgtggccat
    11941 ttcggatgcg aacgtttcaa ttaccgcttg tgcttttttc gaaacagccg gattaaaatc
    12001 attcgcagcg gccatcacaa aaataaacag cccttcagct ttttggttta ataaaaacag
    12061 ccggtgagaa aacgatgatt tttttcgatt aattttcgct tgcttcatcg ctgtttccgc
    12121 tgcccgaagc tctacaaccg ttttgtgctg ctcagcgtga aacgattccg tgccaagtga
    12181 tccaacaaat gaagctatct gcctataagc tttcgccaca gcggcagttt caggcccgcg
    12241 tggattaata agccagccgc tcatgctaat acaccaagat agtgctcctc ctaaaaaaac
    12301 gaggcctgca tgcaaaaaaa ctgcctgtaa attcggagct ggaatgcttg tactcactgc
    12361 aaatgaaaga acaaaaaaca tggctgaagg tcccggaatt tttaaagctc cgaagatata
    12421 aaccgatacg gctccaatca caccaaataa aatagttttt atccactcca cctcagcaaa
    12481 taacgagcct agcgtaaagc acagcgtaat gccaagcaaa gctaaaaata acttaacggc
    12541 acgctgcgcg taaggctcat cttttgtata caaatatgta aacccgccaa tgcttgcaat
    12601 gaggccgtac tgcaaatgac caaacaaaat gcctattaaa atcggcaaac ttgaagacaa
    12661 gcctgagctg attcctctta cccacggaga agaagcgtgg ttaaagcgaa aagcagcttt
    12721 gattcccggc gcttttggag aagcctcaat tttatgatga atttccgtat gattatatgt
    12781 tggtgacttt ttgtcatcat tcaccttgac ctcctccttt ctctccatat atctttcctg
    12841 ttcttaaaac gagataaacc taaaaaaccg ctgttcctat ccgtattgtg tttaggaaca
    12901 gcgcttttta tccttttaag cagcgtacag aaaaaacaaa aagcagatga aacgaatgtc
    12961 tcatctgctt tttactaaca actctactgc ttcttttact aaattttccg tattctcgcc
    13021 tttttcaatg ctttctctta cgcattgttc aaggtttgtg ctgacaataa cacccatcgt
    13081 gcgatcaatt gcgcttctgg ctgctgagag ctgtgtcaca atgtcacggc agtcttttcc
    13141 ttgttccatc atatttaaga cgccttttaa ctgaccttca atacgtttca aacgattttt
    13201 aacttgctta tcatattcca tcatactact acctccgctc atgcatgatt gcgtttgttg
    13261 cttttaagct tacacaagaa gtaaatgtac acacaacttt atacctatta gggtatgatg
    13321 tcaagtaaag gaagttgact ctttttttca cctaggaccc atagacatac aattagacac
    13381 ttcacactcc atttaatctg aatcgtaagt aaggtacaca ccgcaaagta taactcctag
    13441 aatattctga ggccttttga atcttacaga taagacaaaa tgatcatttt tacataattg
    13501 ttaatacttg tttacaccgt atttattgtc ctgtaatcta aacttagcaa tctgagagta
    13561 tagttaagct gtaaaaacga ctacactgga ggtttgagat gagtacatac gggaaaaaat
    13621 taatggcaag tctattcatt atcattgcat gcgttttagg gtttatagga catgcggcgt
    13681 ttttagctcc tgaaaaaacg gaagccgaag caaactatga tgcaacgctt tattttccac
    13741 tggaccgata tcctgaaacc ggcgatcata ttcgcgacgc aattgctgct ggtcactctt
    13801 ccgtttgcac aattgatcgc gatggtgctg aagcaaaccg agaagaatcg ctaaaaggat
    13861 acccaacaaa aacaggctat gaccgtgatg aatggccaat ggctatgtgc gaagaaggcg
    13921 gagcaggagc tgatattcgc tatatttcac cttctgataa ccgcggagcc ggctcatggg
    13981 ttggaaatca gttagaacag tacccagatg gtacacgcgt acaatttatc gtgcaataat
    14041 agctgaacaa gctttacttt gaagaggtga aatcatgaaa acaaaatgga aagaacaaga
    14101 gctaacaatg tcatatcatc agtttacgct atttcaaaaa ggacagccaa agccccatca
    14161 cgactggagt gatgaagata ttcaaaaagg atatgtcgca gacgataaag ctatttcatt
    14221 tgaagccgtg tcgaatacaa aagccattat tgaagtgtgg gtaaacgaaa ctccagacgc
    14281 atcaaaggct gaaaagacca ctcgccttcc ttttcacgtc gaaagcgatg ggattgaaat
    14341 caaaagcgtc ctatctgaaa ctttaagcta tgacattcca aaaggatatt attacgtcac
    14401 atgcctgacc attcctcttc aagaaaaaac aaactcaggc ctttatttag tgaagtattt
    14461 gctgcatttt gaaggtcaat aaaaagaagg cagcttactg taagcagttc aggtaacata
    14521 atcgtaatta cacaggaagt aactcataaa aaaatgagac aaacatattt taagtaaaga
    14581 agatccggac gatgaattgt tcggattttt ttattttgat agtggaacaa gcagtccagc
    14641 tcatgtactc tatttgtttg tctttggagt ggattgattc catcatgtct caatctcttc
    14701 tttgtgaaag atgctaaaat atccatccgt taaagaccat acctaaatta ataataaaat
    14761 gaaaaactac ggctggatat atgcttttcg tctttaaaac gacaaatgcg tagaaaaatc
    14821 ccccaaaaga aaaaagcaaa cttattacca caggaattcc tattgatatg tgcaaaagac
    14881 cgaatccaat actcgtaata aaaacagcgt aaaaagtcga tagactgctt tttaaatgag
    14941 ataacataat tcctctccaa agcacctctt ctaaaattgc atttataatt gagaatacta
    15001 taccgtatat gacgaatgat tttgtatagc ttatatcttc agcgaatagt aaaggtacaa
    15061 aaatcgtagc cgaaccaatc agaccaaaca ttaaaaagta cgttagctga attgaatgct
    15121 gtggcatcac tacacgtttc tgccagtctg gcaatgatgc aaaaagaaat actctttgct
    15181 tggacaacaa aactgaaagc aatagtccac ctataataaa cagaagtaac actctgttta
    15241 caataatttt catcttttta gatagatccc actcaatgaa ataattgctt cctatttgaa
    15301 acatacatgt ccctactaaa aagaatagaa acagagaaac cgttgaacga tatttaaagg
    15361 ttaaagcata gataaaaagc aagcctatga taggcaacag tcccgcagca aataacttat
    15421 aagaaaaaag aataataccc gagaaaaata aacttattat aaagatgagt aatacattta
    15481 ttcgtaaata ttggatataa aactcctcct ttcactgtcc ctctgattcg ctttatggta
    15541 gttggacttt aaccaaacaa attttattca agcaatgtaa ataaactgaa tccatccata
    15601 aaaataaaaa atcgagggta aaacctttgc gatttcaccc tcgatttttc gtactactat
    15661 tccagacact caagacactg accgttctct gtttatttac cgtggtattc ataagtttct
    15721 ttatttgata taaacgggct aatttcacca agtgagaaca ggtggagctc atgcatcgct
    15781 cttgtgcatg ctgtataaaa taaattccgc tcatattcat cttggtaaca ttcactcgac
    15841 gcattataaa tgatgacagc atcaaattca attccttttg ctaagtaagc tggcaggaca
    15901 agcatgcctt tttcaaatga acttgtttct tttgtcagca gtcttgcaga catcgaatca
    15961 ggcaaagctt ggaatgcttg acgactttcc tcagctgttt tagcaataat agcaatcgtt
    16021 ttatagttgc gctgcttcaa ttcattaatt ttgtttatga ttgcctcatg cagctctttg
    16081 acgtcacctg cagccgtcac ggtcggcttc gctccgtttc gattaaacgg atcgattaaa
    16141 tggccgtttg cgaccatatc ccttgaaaac tcgacaattt gacgtgttga gcgatagctt
    16201 cttgtaagag tcagcgtttc tgcttctttc tcttcataca gctcgcttga aagaatggtc
    16261 ggtgcgttca aggcgtgcgc atagatcgct tgattcaagt ctccaagcag cgtcattctg
    16321 gcatgcggaa acagctcttt taaaaatgct agctgaaacg gtgaataatc ttgtgcttca
    16381 tcaataaaca aatagcgaat gacagggtta gctttgcgcc cttcaatttg atcttggata
    16441 tataaataag gcgttacgtc ttcataagac agcttcatgt tcatgactct ttgtaccgtg
    16501 tttttagcag tgtcttccca cccttctacg tgtgaaaaaa gatgatttct ttcaaacagc
    16561 tgcatataga tacggcgaat gtcgataaat ttaagctgct tgatggcctg cttaataaat
    16621 ttcattttct tagctacaac ggctttggct aaatacgctt gttcccgatc gaaatcatca
    16681 aatgagcttt ccgtatagcc ttcctttttc tgcaggtgct gataggcctc tacataatct
    16741 tctttttcta aaagctgaat ttcttcttga acccaatctt ttttacgctc aagtttttca
    16801 agctgattaa tgagtttaag cgcccactcg gctacaagct gcattctgtt agggatggaa
    16861 attcccgtat caagcgagta gaaataagac agcagacgcc gagcagataa cacgatttcg
    16921 ccgcgaaact taatgttttt aaaaatgatg tctttagtag acagctcttc gatatacgca
    16981 tctacctgct ttttaaattc acggcttgct ttgtagcgga tttcctgcat tttttgtttt
    17041 tttgttgcgg catctttctc cgttagcaaa tattcgattt gaacgaacaa atcctccact
    17101 tcaaactcgt ttcctaaccg tccatcagca tattgctgaa acgttgtttg tttcatgttg
    17161 tcttctccaa gctccggaag aacggttgaa acgtagctgt taaaaagcgg atttggtgaa
    17221 aacagcatca tgttttctgc agtcagctgc tttcggtgat gatagagaag ataagccaca
    17281 cgctgaagag ctgccgacgt tttaccactc cctgctaccc cttgaacaat cagcacttta
    17341 tttttttcat ttcgaataat ttggttttgt tctttttgaa tggtcgctac aatacttttc
    17401 atttgactgc ttgcattgtg cccgagcact tcttgaagca gctcgtctcg aatggtaatt
    17461 cctgtatcaa acatactttt aattacgcta ttttcaataa taaactgacg ctttagcttc
    17521 atctcgcctt taatcgcttc acctggcgtt tcgtactgcg cctttcctgg tgaataatca
    17581 taataaagac tcgaaatcgg cgcacgccaa tcataaatta aaaactcttc atctttttga
    17641 tccattaagg agccgacgcc tacataaata gcttccgctt ctttttcacc attttctata
    17701 aaatcaaaac gcccaaaata aggggattgc gttagttttt ttaatgtttt taattgatca
    17761 tacgcttgtc tatgggtaat ttcacgttca gctaaaagtt ctgcctgctg cttaatgctc
    17821 gtaaatgtct caattacgtc atctggctca tccatattaa ccgtaacatc gtcccaaaac
    17881 gtgcttcgca aatcgacaat ttcacctttt acgtttccta cgcttttgcc tatcttttca
    17941 gctttttggc tgatttcttc tactacccaa tcaactcgct gctgttcttc tgttaaattt
    18001 ttatctttca cttatccaca ccccttacac acgttacccg ataacaagac ttgttaccgg
    18061 gtaaaagcga tcattgttta ttttttcaga gtctacatac ttccctacta agttcgtaaa
    18121 tccctgctat gtaaccatat tatgtcttaa tttccaccgt tagtatactg aaaaataatg
    18181 agctccacaa caaaaagggc tgagaaactc tcaagcccct tatctttata agttgttcct
    18241 ctacttatta gctattactg tttccaaaaa gttagcatat gaatgatggc tgtcaccact
    18301 tctactcctt gaaacgccag taaacctgct gctgccgctg taaacaatcc gactaccgct
    18361 tctttaaaaa tgaatggcat tgataccagc tcctttgctt atttaaaata agtagatttg
    18421 ttatcattct actccttttt tcagaatttt caataccatt ttgtcaatga aataaaataa
    18481 aaacgctttc actttatagg aaataaagtg aaagcgcttt ttaagcttcc ttttctaatg
    18541 cattgagcgc aagtgaatat tctaatcctt tccctttttg cacaacgtgc tgcacttttc
    18601 ccatgttgca tgcttcttgc gcactaacag tcataccgtt tgttaacttg aactgcacag
    18661 gttcttccgc aattcgtacg tcgcatgcta aagctaactc ataccctccg ccaaacgtaa
    18721 agtttgtaag aagcgcaatc gtttgtttgg cactgcatga aatacgctga aaaatcgctc
    18781 gagctgctac tgcatgaatc ggctgataaa gccttcctgt ttcttctcgt aggaaagagg
    18841 ttgttctatg atctccgttc attttatagt actctcccat gaatacaata gatccaaccg
    18901 acgaatcgtg ctgcaactca ttcatcgttt cttgcagctc atacatcact tcgctgttca
    18961 aacgattaag catcgactga ttcattcgta taaccgccac atgatttctt ttttctactt
    19021 tgataaatct tttcatcgtt cctccgccct ttcccctgtc gtcctttata aataaagcgc
    19081 tttcaaaata tttcgctttt taatagaaaa gttattttca tagtttcatc atacaacaaa
    19141 ctcataatta tttccattac ttttttaatt ttccgtcaat aatcccatga atcatttcca
    19201 tgtttttatc aaataagacg tgctatagta taaacagcac aaatgcagct gaactcactg
    19261 ataaaaggag taatcactat gaaatacgac ttaacaaaat gggagcctca cggctattct
    19321 tgccctgtgg aagcagccgt tgatgtagtc ggaggcaaat ggaaaagcgt cattttatat
    19381 caattatcta aggaacgccg gcgctttaat gaactgcgca gactcattcc tggcgtaacg
    19441 cagcgaatgc tcacgctgca gcttcgcgaa ctcgagcgcg acggaattat ccaccgcgaa
    19501 gtctataaac aagttccgcc taaagtagaa tattatttaa cgccttttgg agaaactctc
    19561 atccctatta tcgagctgat gtttcaatgg ggatataatc ataccgataa aattgctgaa
    19621 gcgagaattc aggcagaaaa agagtaaaaa gcacgcaaag cgtgcttttt ttatacatgt
    19681 tttcgaatca ataattcttt taacccatac acaaatgtgc tttcaatcgg ctttaactca
    19741 aatgccgggc tccagtccat atgcggatag cggtcaatca gttcagccag cataactttt
    19801 gcttctattc gagcaagcgg tgctcctaaa caaaaatgaa tccctcttcc gaacgccata
    19861 tgtatatttg gctttcggtc gattttaaat acatccgggt cttcaaagta ctgggcgtct
    19921 cggtgcgcgg atcccatcca tggaacgacg tgttcgcccg cttttagttt cttccctgca
    19981 agaaccacat cttcttttac gattcggtga agagcctgaa cgggtgaacg atagcggaga
    20041 acttcttcaa tcgcagaagg caaaagttct ttttctcgtt tgagtcgttc aaaagaagct
    20101 ttatcttcca ttaaacaata aatcgtattt gaaattaagt tcgtggttgt ttcgttgcct
    20161 gcaatcagca gcaaaatgga aaacccgacg atttcttcat ccgttagctg cttcccttct
    20221 tcttttgcct gaagaagcac ggagataata tcatcccctg gatgcgtgcg tttttcttca
    20281 atgattttat aaaaataagt ttcgagctca tcatttgctt tcattttatc ttgctgcaat
    20341 ttttcgagcg tttcgcgttc attattcgac ggacccgcta cgataatatc cgaccaatct
    20401 ttaaactgac gctgatcttc tatcggcacg cctaaaatat cggcaattac cataaccgga
    20461 agcggatagg aaagatgctc gactatatca atatcttcaa gatgctcaac ttcttgtaat
    20521 aattcgtgtg taatacgcgc aattttaggc tcccatgctt tcattgcttt aggcgtaaaa
    20581 gctttattaa caagcgctct catttcagca tgcttaggcg gatcaatatt aattaggctc
    20641 gttcctagag cagtttgtct ttggttttgt ggaggtcttc gatcagaaga aaaaatattc
    20701 ttttgttcta gcacttcttt gaccccgtca tagtgaaaaa catcccatac ctgcctttct
    20761 tcgtcccatt gcacaggcgc gctgtttctc atctcttcat accatgaaaa aggattaaa
//
